Eppendorf New Brunswick -86 °C Freezers G User Manual

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Operating manual

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HEF® and G -86 C Freezers

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on p.Fig.Tab.p

erating manua

September 16, 2011

New Brunswick -86 °C Freezers

HEF® and G

Operating manual

M1288-0058

Revision H

New Brunswick Scientific reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.

Trademarks

Eppendorf® is a registered trademark of Eppendorf AG, Germany.

BioCommand

is a registered trademark of New Brunswick Scientific Co., Inc., USA.

Innova® is a registered trademark of New Brunswick Scientific Co., Inc., USA.

HEF® is a registered trademark of New Brunswick Scientific, Co., Inc., USA.

New Brunswick™, and the New Brunswick Logo™ are trademarks of Eppendorf AG, Germany.

S.M.A.R.T. Plus™ is a pending trademark of New Brunswick Scientific Co., Inc., USA.

Trademarks are not marked in all cases with ™ or

in this manual.

New Brunswick Scientific has attempted to identify the ownership of all trademarks from public records. Any omissions or errors are unintentional.

September 19, 2011 Revision H M1288-0058

HEF® and G -86 °C Freezers — Operating manual

Table of contents

1 Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2.1 Manual conventions used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2.2 Health and safety at work act 1974. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

3 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3.2.1 Inspection of boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3.2.2 Packing list verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3.3 Product versions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.4 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.5 Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.5.1 Warranty registration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.5.2 Extended warranty option . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Table of contents

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.2.1 Occupancy rating (230 V, 50 Hz models only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.2.2 Below ground installations (230 V, 50 Hz models only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.2.3 Installation categories (230 V, 50 Hz models only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.3 Requirements for water-cooled models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.4 Installing the shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

4.5 Lockable freezer handle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5 Operating controls and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.1 Controls and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6.1 Getting started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6.1.1 Plug in . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6.1.2 Turning the freezer On/Off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6.1.3 Alarm/battery activation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.1.4 Testing the alarm monitoring socket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

6.1.5 Vacuum effect . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

6.2 Programming the freezer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

6.2.1 Setting operating temperature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

6.2.2 Setting high alarm setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.2.3 Setting low alarm setpoint. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.2.4 Checking temperature and alarm setpoint settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Table of contents

HEF® and G -86 °C Freezers — Operating manual

6.2.5 Setting the alarm delay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.2.6 Changing lock codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.2.7 Setting the temperature offset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

6.3 Battery backup switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

6.4 Alarm monitoring socket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.1.1 Painted surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.1.2 Panels and shelves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.1.3 Air intake grille and filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.1.4 Heated vent port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.1.5 Door or lid seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.2 Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.2.1 Lubrication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.2.2 Defrosting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.2.3 Removing the inner doors (upright freezers except U725-G) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.2.4 Replacing the inner door (upright freezers except U725-G) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.2.5 Removing the inner doors (U725-G). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

7.2.6 Replacing the inner door (U725-G). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

7.2.7 Electrical components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

8 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

8.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

8.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

8.3 After a mains/power failure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.4 Interior warming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

10 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10.1.1 A2 independent temperature monitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10.1.2 Auto-dialers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10.1.3 Temperature probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10.1.4 Validation packages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10.1.5 Padlock adapter kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10.1.6 CO

10.1.7 Inventory racking systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10.1.8 Chart recorder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10.1.9 New Brunswick BioCommand SFI datalogging software (RS-485 interface) . . . . . . . . . . . . . . . . . . 40

11 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

11.1 Shut down. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

11.2 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

11.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

and LN2 back-up systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

12 Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

HEF® and G -86 °C Freezers — Operating manual

1 Operating instructions

1.1 Using this manual



Carefully read this operating manual before using the device for the first time.

 Also observe the operating manual enclosed with the accessories.

 The operating manual should be considered as part of the product and stored in a location

that is easily accessible.

 When passing the device on to third parties, be sure to include this operating manual.

 If this manual is lost, please request another one. The current version can be found on our

website http://www.nbsc.com

1.2 Danger symbols and danger levels

1.2.1 Hazard symbols

Hazard point Burns

Electric shock Material damage

Operating instructions

Crush

1.2.2 Degrees of danger

The following degree levels are used in safety messages throughout this manual. Acquaint yourself with each item and the potential risk if you disregard the safety message.

DANGER Will lead to severe injuries or death.

WARNIN G May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Symbols used

Example Meaning

 You are requested to perform an action.

Perform these actions in the sequence described.

• List.

References useful information.

Operating instructions

HEF® and G -86 °C Freezers — Operating manual

1.4 Abbreviations used

A Amp

CFC Chlorofluorocarbons

°C Degree Celsius

G Green

HEF High Efficiency

HCFC Hydrochlorofluorocarbon

Hz Hertz

kg Kilogram

lb Pound

m Meter

min Minute

mm Millimeter

N/A Not applicable

rpm Revolutions per Minute (min

ULT Ultra-Low Temperature

V Volt

-1

)

HEF® and G -86 °C Freezers — Operating manual

2 Safety

2Safety

2.1 Intended use

New Brunswick HEF and G (Green) freezers are designed to provide precise, ultra-low temperature environments for cold storage of scientific or medical materials. They are designed to provide ultra-low temperature sample storage from -50 °C to -86 °C at 32 °C maximum ambient operating temperature.

2.2 Warnings for intended use

2.2.1 Manual conventions used

Risk of material damage

 This equipment must be operated as described in this manual.

NOTICE!

 Please read the entire operating manual before attempting to use this equipment. If

operational guidelines are not followed, equipment damage may occur.

Risk of personal injury

 Do not use this equiment in a hazardous atmosphere or with hazardous materials for which

CAUTION!

the equipment was not designed.

 Please read the entire operating manual before attempting to use this equipment. If

operational guidelines are not followed, personal injury may occur.

Risk of personal injury

 Crush Warning messages alert you to specific procedures or practices regarding heavy

CAUTION!

objects which, if not followed correctly, could result in serious personal injury.

Risk of personal injury

 Flammable warning messages alert you to possible risks of of personal injury and equipment

WARNING!

damage: protect the system from sparks and flames.

Safety

2.2.2 Health and safety at work act 1974

(FOR THE UNITED KINGDOM)

New Brunswick Scientific, as manufacturers and suppliers of laboratory equipment, are obliged under the terms of the above Act to provide our users with instructions on the safe installation, operation and maintenance of our equipment.

Our equipment is designed to acceptable standards and does not entail any hazard if used, as advised in the attached instructions.

The following safety precautions should be observed by all personnel using this equipment:

1. Read and understand this manual. If in doubt, contact your local New Brunswick sales office.

2. Do not remove any covers. There are no operable controls other than those referred to in this manual. There are voltages in excess of 41.5 volts AC behind the covers.

3. Use freezer gloves at all times when loading or unloading the equipment. The temperature of operation is such that direct contact with the cold contents or inside the equipment can burn unprotected skin.

4. Observe good housekeeping practices, at all times keeping the equipment and the adjacent areas clean, dry and uncluttered.

5. Should any malfunctions occur or be suspected, immediately call a qualified service engineer to investigate.

HEF® and G -86 °C Freezers — Operating manual

6. The hydrocarbon (Group A3) refrigerants used in these freezers are flammable and therefore appropriate attention must be paid to avoid leaks and to keep the freezer away from sparks and flames.

Any person involved with working on or entering the refrigeration circuit should hold a current and valid certificate from an industry-accredited assessment authority which authorizes his/her competence to handle refrigerants (including hydrocarbons) safely in accordance with local regulations and legislation.

Safety

HEF® and G -86 °C Freezers — Operating manual

3 Product description

3.1 Main illustration

Abb. 1: Upright freezer - Side an d front views

12 3

Product description

76891011

Fig. 1: Upright freezer - Side and front views

1 Heated vent port 2 Door handle (lockable)

3 Control panel/display 4 Model label

5 Battery switch behind lockable panel 6 On/Off circuit breaker behind lockable panel

7 Transport castors 8 Chart recorder (optional)

9 Air filter grille 10 Quarter turn fastener

11 Specification plate

HEF® and G -86 °C Freezers — Operating manual

Abb. 2: Chest freezer - Front view

4 5 6

Fig. 2: Chest freezer - Front view

1 Heated vent port (inside compressor housing) 2 Lockable handle

3 Control panel/display 4 Battery switch (alarm) behind lockable panel

5 On/Off circuit breaker behind lockable panel 6 Chart recorder (optional)

Product description

7 Quarter turn fasteners 8 Air filter grille

9 Braked castors (front)

3.2 Delivery package

3.2.1 Inspection of boxes

Inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage to the carrier and to your local New Brunswick Sales Order Department immediately.

3.2.2 Packing list verification

Unpack your order, saving the packing materials for possible future use. Save the operating manual for instruction and reference. Verify against your New Brunswick packing list that you have received the correct materials, and that nothing is missing. If any part of your order was damaged during shipping, is missing, or fails to operate, fill out the "Customer Feedback" form,

NOTICE!

available online at www.nbsc.com/CustomerFeedback.aspx

Risk of material damage

 Vacuum insulation panels are used in the construction of Innova U725G and U410 HEF,

U570 HEF, and C660 HEF model freezers. Inspect the cabinet panels for punctures or other damage that compromises the integrity of the product.

 These panels are mounted in the cavity against the steel outer wall of the freezer. Any drilling

or puncture to the outer wall could release the vacuum from the panel, resulting in impaired freezer performance.

 Any unauthorized punctures or other damage deliberately made to the cabinet walls will

invalidate the warranty.

HEF® and G -86 °C Freezers — Operating manual

3.3 Product versions

3.3.1 Introduction

This manual provides the user with the necessary information for installation and operation of New Brunswick’s energy saving HEF and G range of Ultra-Low Temperature freezers: Innova U725G, and the HEF models. It also provides some preliminary user maintenance information.

This manual covers the following freezer models:

Model (230 V, 50 Hz) Capacity

Innova U725-G Air-cooled 725 liters (25.6 cubic feet)

Innova U725-G Water-cooled 725 liters (25.6 cubic feet)

U410 HEF 410 liters (14.5 cubic feet)

U570 HEF 570 liters (20.1 cubic feet)

C660 HEF 660 liters (23.3 cubic feet)

All the above mentioned HEF and G freezers are totally free of CFCs (Chlorofluorocarbons) and HCFCs (Hydrochlorofluorocarbons). They use HCs (Hydrocarbons) as refrigerants.

The use of Hydrocarbons as refrigerant is prohibited in the United States.

Hint!

The following HEF freezer models utilize HFCs (Hydrofluorocarbons) as refrigerants, and are offered for the United States market.

Model (120 V, 60 Hz) Capacity

U410 HEF 410 liters (14.5 cubic feet)

U570 HEF 570 liters (20.1 cubic feet)

Product description

3.4 Features

Model (208 - 230 V, 60 Hz) Capacity

C660 HEF 660 liters (23.3 cubic feet)

The freezers are manufactured using high quality steel and electronics for long life. The HEF and G-Series freezers have many features designed to provide ease of use and maintanence, security and reliability to your ultra-low temperature storage needs. They are built to stringent regulatory requirements for safety and environmental friendliness and disposability, and they are CE and UL certified.

Features include:

• Designed to be exceptionally energy efficient and eco-friendly.

• A setpoint keypad and digital temperature readout are provided on a control panel, located on

the door of the upright freezers and on the console on the right side of chest freezers. Controls for setting the freezer temperature and alarm warning setpoints are enabled by an electronic lock. A unique code, which is selected by the user through the keypad, sets this lock. The code can be changed at any time, provided the current setting of the lock is known.

On delivery, the lock code is "0000" (unlocked).

• Indicator lamps on the control panel provide warnings of power loss, system failure,

exceeding High/Low temperature setpoints, low battery voltage, and filter blockage. There is also an LED to indicate when the freezer is under remote control via the RS-485 optional computer interface.

• Heavy-duty commercially available compressors provide rapid temperature pull-down and

recovery after door opening.

HEF® and G -86 °C Freezers — Operating manual

• A heated port with ice-clearing plunger prevents vacuum formation enabling the outer door to

be easily opened at anytime.

• All interior panels and shelves are made of high grade corrosion-resistant stainless steel,

making them durable and easy to keep clean and sterilize.

• Heavy duty castors enable easy installation and relocation.

• Two access ports allow easy addition of sensors, or of back-up systems that can provide

cooling protection for your samples in the case of a power outage or other system failure.

• An alarm contact is provided for connection to an external monitoring device or system.

• The freezers can be fitted with an optional 7-day circular chart recorder to provide

independent temperature recording.

• An automatic reset restarts freezers at random 15 second intervals to protect the

microprocessor controller from damage by electrical spikes caused by multiple freezers turning on at once.

• Lockable freezer handles on outer door provide added security against unauthorized users.

• Multiple accessories are offered, including CO

systems, external voltage stabilizer, inventory racking, and more.

3.5 Warranty

New Brunswick Scientific equipment is protected by a comprehensive warranty. The warranty covers faulty components and assembly, and our obligation under this warranty is limited to repairing or replacing the instrument or part thereof, which shall prove to be defective after our examination.

The warranty does not cover loss of time or materials, such as the loss of biological or biochemical by-products caused by any work interruption resulting from equipment failure; it does not extend to equipment that has been subject to misuse, neglect, accident or improper installation or application; nor does it cover any machine that has been repaired or altered by anyone other than an authorized factory-trained service representative, without prior written

Product description

approval from your local New Brunswick sales office or distributor.

Expendable items such as bearings and seals, lamps, probes, sensors, glass, filters, single-use vessels, etc., are not covered.

The warranty begins on the date the equipment ships from New Brunswick Scientific or an authorized distributor and extends through the period indicated in the chart below:

/LN2 back-up systems, remote monitoring

3.5.1 Warranty registration

3.5.2 Extended warranty option

Instrument Parts Warranty Labor Warranty

Freezers ULT Freezers 5 years; vacuum

insulation panels: 12 years

Accessories

Chart recorders, CO2/LN2 back-up systems, etc.

To register your warranty, complete the online form at www.nbsc.com Help? tab.

A variety of service plans are offered to help minimize downtime from unexpected malfunctions in equipment operation. Speak to your New Brunswick sales representative for more information.

1 year 1 year

2 years

under the How Can We

HEF® and G -86 °C Freezers — Operating manual

4 Installation

4.1 Preparing installation

Risk of personal injury

 DO NOT attempt to lift any freezer by hand. Preferred lifting for loading and unloading is by

WARNING!

NOTICE!

4.2 Selecting the location

mechanical lifting equipment.

Risk of material damage

 Maintenance, adjustment and repair work should be carried out only by QUALIFIED,

EXPERIENCED personnel who have been AUTHORIZED to undertake such work by New Brunswick Scientific or its authorized agents.

 Failure to use authorized service agents will invalidate the warranty.

All freezers are mounted on castors for ease of movement. U725-G model freezers have feet that provide both a leveling feature and a locking feature to stop the freezer from rolling once it is in place. HEF upright freezers and chest freezers have locking front castors; locking front castors do not provide leveling adjustment, so the site chosen for the freezer must have a flat, level floor.

Position the freezer to allow disconnection to be made in respect to removal of the plug or appliance coupler, also the free air entry through the intake grille in the front and free air exit from the back. Provide a clearance of at least 150 mm (6 in) on all sides.

For efficient temperature control, the freezer should be placed in a shaded area, away from sources of excessive heat. For maximum cooling capability, the product should be located in an air-conditioned room.

Installation

4.2.1 Occupancy rating (230 V, 50 Hz models only)

This equipment has a category A1 - B1 occupancy rating, with a refrigerant charge of less than

0.15 kg per sealed system.

Systems charged with less than 0.15 kg may be installed in any size room as long as adequate ventilation is provided, in order to remove rejected heat from the freezer and to vent any sudden loss of refrigerant in case of system failure.

4.2.2 Below ground installations (230 V, 50 Hz models only)

We recommend allowing at least 18 m refrigerant concentration from exceeding 20 % of the Lower Flammability Limit (LFL) in the event of a sudden loss of refrigerant into the room.

Basements and cellars must have adequate ventilation for the removal of heat rejected from the freezer(s).

(23.5 yd3) room volume for each freezer, to keep the air/

HEF® and G -86 °C Freezers — Operating manual

4.2.3 Installation categories (230 V, 50 Hz models only)

Category Examples Requirements

A1 (domestic/ public) Hospitals

B1 (commercial/ private) Business or professional

4.3 Requirements for water-cooled models

If your freezer has a water-cooled condenser, the following are water supply and drainage requirements:

Minimum flow requirements

Max Inlet pressure 10 bars

Min Inlet pressure 1 bar

Prisons

Theatres

Schools

Supermarkets

Hotels

Dwellings

offices

Shops

Restaurants

Laboratories

General manufacturing

3.8 liters/minute

< 0.15 kg refrigerant per sealed system

Installation

Maximum supply

temperature

Minimum supply

temperature

Connection size Inlet : 15 mm x ½” BSPT

Water quality Water must be clean and free from particles that could cause

Drainage requirements

Typical Flow Rates At a setpoint of -85 °C, an ambient temperature of 25 °C, and a

Water consumption will increase as water temperature increases.

The condenser must never be allowed to freeze during operation. If, during normal cycling,

water temperature approaches 6.0 °C, this must be checked.

This installation requires checking the high stage discharge pressure and may require an adjustment of the water regulating valve; both operations MUST be carried out by a qualified engineer.

25 °C

7.0 °C

Outlet: 15 mm x ½” BSPT

blockage in the regulating valve or heat exchanger. A suitable inline strainer must be placed in the inlet pipe if there is any doubt about the cleanliness of the supply. (Minimum filter requirement is 60 mesh 0.25 mm aperture).

A recirculated cooler return line and a main supply line to the waste drain are required.

water inlet temperature of 20 °C:

47 L/hr for the U725-G.

HEF® and G -86 °C Freezers — Operating manual

4.4 Installing the shelves

Model U410 HEF and U570 HEF freezers are fitted with four adjustable shelves. Model U725 freezers are fitted with two adjustable shelves. These can be positioned in 12.7 mm (½ in) steps anywhere throughout the freezer.

To effectively utilize racks within the freezer, be sure to position them so that each shelf is aligned with the bottom of each inner door.

Perform the following steps to install the shelves:

1. Ensure that the freezer is turned off and unplugged.

2. Remove the protective plastic coating from the shelf.

3. Position the four shelf clips evenly within the freezer by squeezing the clip, then inserting it

into the shelf support within the freezer.

4. Place the shelf in the freezer, making sure all four shelf clips are supporting the weight of the

shelf.

To readjust the shelf or shelf clips, gently squeeze the shelf clip to release it from the side of the freezer, then reposition it as needed.

4.5 Lockable freezer handle

Freezers are supplied with lockable handles.

The C660 HEF handle is fitted with a quarter turn key lock.

The upright freezer handle is fitted with barrel locks (push in and turn key to lock, turn key to unlock; the barrel will only lock when a key is turned to the lock position). The barrel lock may be removed from the upright freezer handle if the lock feature is not required.

An optional padlock adaptor can provide extra security by allowing the addition of a customer-supplied padlock to secure the freezer handle.

Removing the upright freezer handle barrel lock

Perform the following steps to remove the barrel lock from the upright freezer handle, if the lock feature is not required:

Installation

1. Open the freezer door and place the freezer handle in closed position.

HEF® and G -86 °C Freezers — Operating manual

2. Remove the two screws from behind the lock barrel.

Installation

1 Freezer handle 2 Screw (1 of 2)

3 Lining plate 4 Freezer door wall

3. Remove the lining plate and lock barrel.

4. Insert the plastic blanking plug supplied.

5. Insert the lining plate and screw in the two screws.

It is important that the handle lock lining plate be installed at all times.

Hint!

Risk of material damage

 DO NOT SLAM THE DOOR WITH THE HANDLE IN THE CLOSED POSITION.

NOTICE!

6. Place freezer handle in open position and close freezer door.

The door handle has a cam action to pull the door closed and a reverse cam action to break the seal so the door can be opened. When closing the outer door, ensure that the cam is engaged for correct operation. The initial vacuum inside the cabinet may cause the door to appear closed, but when the vacuum releases, the door will open. Always ensure the handle is properly engaged. It is important that the heated vent port is kept clear. This will avoid putting undue stress on the handle mechanism.

HEF® and G -86 °C Freezers — Operating manual

5 Operating controls and function

5.1 Controls and function

Operating controls are located on a control panel mounted in the door of the upright freezers, and on the console on the right side of chest freezers.

Every New Brunswick freezer is equipped with S.M.A.R.T. Plus™ diagnostic software, to help identify the cause of a fault or setpoint variance.

This section describes the controls and function of the control panel, (see Fig. 3 on p. 17).

Abb. 3: Display panel & keypad

TEMPERATURE

Fig. 3: Display panel & keypad

REMOTE-CONTROL

TEMP-ALARM

FILTER-CLEAN BATTERY-LOW

POWER-FAIL FAULT

1315

23456

78 90EC

ALARM

SET

TEMP

LOW

ALARM

HIGH

ALARM

LOCK

CODE

CHANGE

TEST/ MUTE

10927543 6

Operating controls and function

Temperature °C Display

Item Name Function

1 TEMPERATURE °CThe digital display normally shows the current internal

temperature of the freezer. Temperature is displayed in 1°C increments.

High alarm/low alarm

Item Name Function

2 & 8 [HIGH/LOW]

TEMP-ALARM

The TEMP-ALARM illuminates and the audible alarm sounds if the freezer’s internal temperature is above/below the user-selected alarm setpoints. The LED illuminates as soon as the setpoint is passed. The high temperature audible alarm has a programmable delay (see Setting the alarm delay on p. 25). After the temperature returns to the normal range, the TEMP-ALARM switches off and the audible warning stops.

The audible alarm can be silenced by pressing the ALARM TEST/MUTE KEY. If the temperature

Hint!

has not returned to normal range after the programmed time period, the audible warning will sound again. This pattern will continue to repeat until the temperature returns to normal.

HEF® and G -86 °C Freezers — Operating manual

Operating controls and function

Temp-alarm light

Item Name Function

8 TEMP-ALARM Should a mains/power failure cause the temperature to surpass

the alarm setpoint, the TEMP-ALARM illuminates. (The audible alarm will already be sounding due to the mains/power failure). The TEMP-ALARM lamp will extinguish when the temperature returns to normal set range. Cancel the TEMP-ALARM by pressing the ALARM TEST/MUTE KEY.

Power fail light

Item Name Function

3 POWER-FAIL Illuminates if the mains/power supply fails, flashing at

approximately 10-second intervals, accompanied by an audible alarm. When mains/power is restored, the indicator goes off and the audible alarm stops. (The battery must be switched on and charged for this indicator to operate.)

Fault light

Item Name Function

4 FAU LT Illuminates if there is a system failure within the freezer.

Interfacing with the S.M.A.R.T. Plus™ diagnostics via the control panel, the fault can be determined (see Error messages on p. 34). System failure is accompanied by an audible alarm. Correction of the fault extinguishes the light and audible alarm.

Battery low light

Item Name Function

5 BATTERY-LOW With mains/power ON: illuminates if battery voltage is below 6

volts, flashes when voltage drops to 5 Volts. With mains/power OFF: if battery voltage drop below 5.5 Volts, this fault indicator will stop functioning.

Filter clean light

Item Name Function

6 FILTER-CLEAN Illuminates, accompanied by an audible alarm, to indicate a

blocked or dirty filter. Filter is located on the front at the bottom of all freezers. Remove by turning the two thumbscrews on the filter holder ¼ turn. Clean filter by washing in mildly soapy water, then air dry. If filter warning light does not go out after replacing the cleaned filter, contact your local New Brunswick service representative.

Remote control light

Item Name Function

12 REMOTE

CONTROL

Indicates when freezer is operating under remote computer control via the optional RS-485 interface port and New

Brunswick's BioCommand data logging software.

SFI software, or other laboratory

HEF® and G -86 °C Freezers — Operating manual

The keypad controls are locked when the freezer is under remote control.

Hint!

Set temp key

Operation in normal mode with LOCK lamp off.

Item Name Function

7 SET TEMP Displays current temperature setting. Used to change

temperature settings.

High alarm/low alarm keys

Operation in normal mode with LOCK lamp off.

Item Name Function

2 HIGH-ALARM Displays current high alarm temperature setting.

2 LOW-ALARM Displays current low alarm temperature setting.

Lock key

Operation in normal mode with LOCK lamp off.

Operating controls and function

Item Name Function

9 LOCK Locks and unlocks the control panel for programming

sequence.

Code change key

Operation in normal mode with LOCK lamp off.

Item Name Function

10 CODE CHANGE Used to change freezer lock codes. Inactive in normal mode.

Alarm test/mute key

Operation in normal mode with LOCK lamp off.

Item Name Function

11 ALARM TEST/

MUTE

Using the TEST/MUTE button does NOT cancel remote alarm monitor socket switching.

Hint!

Sounds the audible alarm. If the audible alarm is on due to a fault condition, press this key to silence the alarm. The lamp LED lights can also be tested by pressing this key. The lights should all illuminate and the display should read “8888”.

"E" key

Operation in normal mode with LOCK lamp off.

Item Name Function

13 E Used to enter data when programming.

HEF® and G -86 °C Freezers — Operating manual

Operating controls and function

"C" key

Operation in normal mode with LOCK lamp off.

Item Name Function

14 C Used to cancel data when programming.

Numerical keys

Operation in normal mode with LOCK lamp off.

Item Name Function

15 NUMERICAL

KEYS (1-0)

Used to input data when programming. Keys 8 and 9 are also used to program alarm delays (see Setting the alarm delay on p. 25).

HEF® and G -86 °C Freezers — Operating manual

6 Operation

6.1 Getting started

Risk of personal injury

 BEFORE connecting the freezer to the mains/electrical supply, make sure that the mains/

WARNING!

6.1.1 Plug in

power supply matches the requirements of the equipment. Check the specification plate (located on the side of the freezer) for the electrical requirements. The equipment should be connected to an earthed/grounded outlet socket.

Once you have verified that the mains/power supply matches the electrical requirements of the freezer, connect the product to the mains/power supply using the mains/power cord provided.

Risk of personal injury

 If the freezer’s voltage rating does not match your mains/electrical supply, or if the plug on the

WARNING!

mains/power cord does not fit the outlet, do not plug the freezer in.

 Contact your laboratory supervisor, safety officer, or qualified service or electrical engineer.

Risk of material damage

 Some freezers are supplied with more than one removable mains/power cord. Utilize the cord

NOTICE!

6.1.2 Turning the freezer On/Off

that matches your power receptacle. Check the voltage rating plate on the side of the freezer, to confirm that the freezer is compatible with your laboratory mains/power supply.

Operation

WARNING!

Hint!

Risk of personal injury

 The On/Off circuit breaker and battery switch are fitted with IP65 plastic covers, to prevent a

possible source of ignition. These covers must not be removed. If one of the covers needs to be replaced, the replacement must be performed by a qualified and authorized person. Failure to observe this safety warning will invalidate the warranty and could result in a dangerous situation in the event of a failure.

The ON/OFF circuit breaker is located within the lockable panel at the bottom right-hand corner of the upright freezer or to the left of the control panel on the chest model.

To remove the lockable panel and turn the circuit breaker and battery switch On/Off:

1. Insert and turn the key (provided) one quarter turn to the right.

The key can be removed to prevent access.

2. Remove the panel.

3. Set the ON/OFF circuit breaker and battery switch to the I (ON) position.

The temperature display illuminates immediately.

Operation

HEF® and G -86 °C Freezers — Operating manual

Abb. 4: Upright freezers switch location

Fig. 4: Upright freezers switch location

1 Battery switch 2 On/Off circuit breaker switch

Abb. 5: Chest freezers switch location

Fig. 5: Chest freezers switch location

1 Battery switch 2 On/Off circuit breaker switch

The compressors will not operate for approximately three minutes after connection of the mains/

Hint!

power supply, because there is an automatic delay device in the circuit. Temperature and alarm settings can be adjusted immediately.

6.1.3 Alarm/battery activation

The equipment is delivered with the battery deactivated. The Power Fail alarm is activated by the battery rocker switch within the lockable panel, which is located at the bottom right-hand corner. The switch is labelled I (ON) and O (OFF) (see Fig. 4 on p. 22) and (Fig. 5 on p. 22).

 To activate the alarm, place the battery switch in the I position.

Failure to turn on the battery switch may lead to a discharged battery, low battery alarm

Hint!

indication, and/or a disabled alarm system.

 After activating the alarm, test its operation by pressing the ALARM TEST/MUTE key on the

display.

The audible alarm should sound.

The ALARM TEST/MUTE key also tests the LED lamps. All of the LEDs should light up together when the button is pressed.

Pull down time to -86 °C will be dependent on the freezer size and model (see Specifications on

Hint!

p. 36). The alarm will sound every 30 minutes until the temperature setpoint is reached. Use the ALARM TEST/MUTE key to mute the alarm during this initial pull-down period.

HEF® and G -86 °C Freezers — Operating manual

If the freezer is turned off during the initial pull-down period, the alarm will activate 30 minutes after switching it back on.

The factory-set temperature is -80°C.

6.1.4 Testing the alarm monitoring socket

The freezer is fitted with a remote alarm socket for testing power-fail and low battery alarms, and for connection to an external building monitoring system or optional auto-dialer, (see Alarm monitoring socket on p. 26). To test the alarm monitoring socket.

 Tur n o f f (O) the ON/OFF circuit breaker.

This will test the POWER FAIL and ALARM output at the same time.

The battery must be switched on to test the POWER FAIL. The remote alarm facility provides voltage-free contacts rated at 1 amp, 24 volts maximum.

6.1.5 Vacuum effect

After closing the freezer door, a vacuum may be created. Before the door can be opened again, it may be necessary to wait two or three minutes for the vacuum to be released by the vent port. Do not try to force the door. During the release of the vacuum, a slight hissing may be heard. In order to minimize vacuum formation, the vent heater assembly has a spring-loaded plunger to clear ice from the inside of the vent.

Be careful not to place a rack directly against the vent, as this will inhibit the plunger’s ability to

Hint!

operate correctly (see Heated vent port on p. 29).

Operation

6.2 Programming the freezer

Set the freezer to any temperature within the range from -50 °C to -86 °C.

All temperature setpoints are automatically negative °C.

Hint!

6.2.1 Setting operating temperature

To set the operating temperature for the freezer:

1. Press the LOCK key.

The LOCK lamp will flash if a lock code (password protection) is required, (see Changing lock

Hint!

codes on p. 25).

The LOCK lamp will illuminate, indicating the system is unlocked and parameters can be changed.

2. Press the SET TEMP key.

Its indicator will flash and the display will indicate 0.

3. Using the numerical keys, enter a new temperature (from -50 °C to -86 °C).

The temperature selected will appear in the TEMPERATURE display.

4. When the correct temperature is displayed, press the E key to enter the data. To set the high

alarm setpoint skip to (see Setting high alarm setpoint on p. 24), step 2.

The SET TEMP lamp will go off.

5. Press the LOCK key to exit programming.

The LOCK lamp will go off and the freezer will return to normal mode.

Press the C key to clear the display during programming.

Hint!

Operation

HEF® and G -86 °C Freezers — Operating manual

6.2.2 Setting high alarm setpoint

The high alarm setpoint may not be warmer than -10 °C and may not be less than within +5° of the operating temperature. The default setting is +5° from the temperature setpoint.

1. Press the LOCK key.

The LOCK lamp will flash if a lock code (password protection) is required, (see Changing lock

Hint!

codes on p. 25).

The LOCK lamp will illuminate, indicating the system is unlocked and parameters can be changed.

2. Press the HIGH ALARM key.

Its indicator will flash and the display will indicate 0.

3. Using the numerical keys, enter a new alarm setpoint temperature.

The selected temperature will appear in the TEMPERATURE display.

4. When the correct temperature is displayed, press the E (Enter) key to enter the data. To set

the low alarm setpoint skip to (see Setting low alarm setpoint on p. 24), step 2.

The HIGH ALARM indicator will turn off.

5. Press the LOCK key to exit programming.

The LOCK lamp will go off and the freezer will return to normal mode.

Press the C key to clear the display during programming.

Hint!

6.2.3 Setting low alarm setpoint

The low alarm setpoint may not be colder than -91 °C and may not be more than within -5° of the operating temperature. The default setting is -5 °C from the temperature setpoint.

1. Press the LOCK key.

The LOCK lamp will flash if a lock code (password protection) is required, (see Changing lock

Hint!

codes on p. 25).

The LOCK lamp will illuminate, indicating the system is unlocked and parameters can be changed.

2. Press the LOW ALARM key.

Its indicator will flash and the display will indicate 0.

3. Using the numerical keys, enter a new alarm setpoint temperature.

The selected temperature will appear in the TEMPERATURE display.

4. When the correct temperature is displayed, press the E (Enter) key to enter the data.

The LOW ALARM indicator will turn off.

5. Press the LOCK key to exit programming.

The LOCK lamp will go off and the freezer will return to normal mode.

Press the C key to clear the display during programming.

Hint!

HEF® and G -86 °C Freezers — Operating manual

6.2.4 Checking temperature and alarm setpoint settings

To view the currently set operating temperature, high alarm setpoint, or low alarm setpoint for the freezer.

 Press the SET TEMP key, HIGH ALARM key, or the LOW ALARM key and read the display.

If you press the SET TEMP, HIGH ALARM or LOW ALARM key while the LOCK key lamp is

Hint!

flashing, the display will read ---- , which indicates that the freezer is locked.

6.2.5 Setting the alarm delay

The HIGH ALARM audible alarm and the REMOTE ALARM monitoring socket can be programmed to a time delay set between 0 and 40 minutes.

The default time delay is 30 minutes. If the time delay is set to 0 minutes, the system will program it as 15 seconds.

Press KEY 8 to display the High Temperature audible alarm delay, and press KEY 9 to display

Hint!

the Remote Alarm Socket switching delay.

To set the audible HIGH ALARM delay (KEY 8):

1. Press the LOCK key.

The LOCK lamp illuminates, indicating the system is unlocked and parameters can be changed.

2. Press keypad button 8.

pp flashes on the display.

3. Enter the desired value (e.g., press keypad buttons 1 and 0 to designate 10 minutes).

4. Press the E (Enter) key.

The LOCK lamp goes out.

To set the REMOTE ALARM socket time delay (KEY 9):

1. Press the LOCK key.

The LOCK lamp illuminate, indicating the system is unlocked and parameters can be changed.

2. Press keypad button 9.

pp flashes on the display.

3. Enter the desired value (e.g., press keypad button 5 to designate 5 minutes).

4. Press the E (Enter) key.

The LOCK lamp goes out.

If the number entered is valid, --- flashes on the display, the value has been stored, and the LOCK lamp goes out. (This is a one-shot operation.)

If the number entered is out of range, –EE– shows on the display and the operation will need to be repeated using a valid number.

Operation

6.2.6 Changing lock codes

If you enter a lock code when there is none, or if you replace an existing lock code with a new

Hint!

one, take note of the new code before you enter it.

If the code is forgotten, you will need to contact Customer Service to regain access to the programming mode of the freezer.

Operation

HEF® and G -86 °C Freezers — Operating manual

The freezer is delivered unlocked. To change the code, the freezer must be unlocked. If a lock code has already been set (indicated by the LOCK lamp flashing when the LOCK key is pressed), that same code must be entered to unlock the freezer. When the freezer is unlocked, the LOCK lamp is on (not flashing).

Once the freezer is unlocked, follow these steps to set a new lock code:

1. Press the CODE CHANGE key.

The lamp will flash and the display will go blank.

2. Using the numerical keys, enter the new four-digit number. Check it on the display.

3. Press the C key to cancel the entry if the display shows it to be incorrect, then enter the

correct number.

4. When the number is correct, record the new number somewhere secure. Then press the E

(Enter) key.

The CODE CHANGE indicator will turn off.

5. Press the LOCK key.

Its indicator lamp will turn off.

The freezer now has a new lock code. If at any time you wish to change this code, you will have to enter this code to unlock the system before you can enter a new code.

Setting the lock code to 0000 disables the lock completely. With the 0000 code, you would need only press the LOCK key to reprogram the freezer.

6.2.7 Setting the temperature offset

The temperature offset function enables to add a temperature offset to the factory defined temperature settings.

1. Press the LOCK Key.

2. Press the C key to access the offset function.

3. Press 0, 1, 2, 3, or 4 key to set the offset in degrees.

4. Press the ENTER key to confirm selection.

Set temperature offset to "0" for no offset.

Hint!

6.3 Battery backup switch

This is a rocker switch labeled I/O behind the locked front panel. In the O position, the battery is disconnected. This position should only be used while in transit, in storage, or to change the battery.

At all other times the switch should be kept in the I position for the battery to be charged, and for the alarm function to be available in the event of mains/power failure. (Failure to set the switch may result in impaired battery life, and the alarm will not trigger if the mains/power fails.)

With the battery switch on, during a mains/power failure, the internal freezer temperature will be displayed at ten-second intervals, and the audio alarm will also sound. The audible alarm may be muted by pressing the ALARM TEST/MUTE key on the control panel, but will sound again after 30 minutes if the fault has not been corrected. Pressing the same button again will mute the alarm for an additional 30 minutes; the pattern will continue to repeat until the initial problem is corrected.

6.4 Alarm monitoring socket

The freezers are provided with an alarm monitoring socket at the rear of the freezer and a matching plug for external monitoring. This plug can be connected either to a central monitoring system such as a building management system, or to a remote alarm via an auto-dialer.

HEF® and G -86 °C Freezers — Operating manual

123

Abb. 6: Upright freezers alarm monitoring socket

Fig. 6: Upright freezers alarm monitoring socket

1 RS-485 connector (optional) 2 Alarm monitoring socket

3 Mains/power socket

Abb. 7: Chest freezers alarm mo nitoring socket

Operation

Fig. 7: Chest freezers alarm monitoring socket

1 RS-485 connector (optional) 2 Alarm monitoring socket

3 Mains/power socket

NOTICE!

The configuration of the socket is shown in (Fig. 8 on p. 28) and (Fig. 9 on p. 28), as viewed from the rear of the freezer. Within the freezer, the socket is connected to voltage-free contacts rated at 24 volts, 1 amp. In normal operation, with the mains/power on, pin 1 is connected to pin 2 (N/ C), and in the alarm condition, with mains/power off, pin 1 is connected to pin 3.

The High Temperature Alarm output to the Remote Alarm Monitoring Socket can be programmed to a set time delay (see Setting the alarm delay on p. 25).

Risk of material damage

 Hazardous voltages must not be connected to the remote alarm socket. Max Rating 24 V 1 A.

Operation

HEF® and G -86 °C Freezers — Operating manual

Abb. 8: Remote alarm socket - upright f reezer

Fig. 8: Remote alarm socket - upright freezer

Abb. 9: Remote alarm socket - chest free zer

Fig. 9: Remote alarm socket - chest freezer

HEF® and G -86 °C Freezers — Operating manual

7 Maintenance

7.1 Cleaning

Risk of material damage

 Maintenance, adjustment and repair work should be carried out only by QUALIFIED,

NOTICE!

7.1.1 Painted surfaces

7.1.2 Panels and shelves

7.1.3 Air intake grille and filter

EXPERIENCED personnel who have been AUTHORIZED to undertake such work by New Brunswick Scientific or its authorized agents.

 Failure to use authorized service agents will invalidate the warranty.

All exterior paint work and inner doors should be cleaned using a solution of mild detergent in water. Do not use abrasive cleaners or solvents.

The interior panels and shelves are made of stainless steel. They may be cleaned and sterilized.

Maintenance

NOTICE!

7.1.4 Heated vent port

Hint!

Risk of material damage

 Serious damage to the freezer may result if the air intake is blocked. Check that there is no

obstruction of the airflow to the freezer. The air intake filter must also be cleaned regularly.

 Remove the filter from behind the grille by turning the thumbscrews ¼ turn and opening grille

downward. The filter should be washed in warm soapy water and left to air dry before replacing.

The air intake grille must be cleaned regularly to keep it free from dust and debris. Under normal conditions, clean the grille once every three months. If the area around the freezer is very dusty or dirty, clean the grille more often.

 Brush the grille with a soft brush and, if a vacuum cleaner is available, vacuum the dust from

the grille.

There is an electrically-heated vent port in the freezer which must not be allowed to become blocked or sealed off.

Over a period of a few weeks, depending on how often the freezer is being used, a small mushroom of ice will form around the end of the vent port. If the vent port is allowed to become blocked, a vacuum will be created when the door is closed. It will not be possible to open the door or lift the lid until the vacuum has leaked away through the seal, which can take up to two hours due to the high quality of the seals.

The vent port is located on the left-hand side of the freezers.

 If the door cannot be opened, clear the vent port by pressing the manual plunger on the

outside of the air vent.

7.1.5 Door or lid seal

Maintenance

HEF® and G -86 °C Freezers — Operating manual

123

1Plunger 2Cover

3 Freezer outer wall

Be sure to treat the door or lid seal with care. Avoid damaging this seal in any way. The freezer cannot operate properly with a defective seal.

 It is advisable to wipe both the seal and the surface against which it seals with a soft dry cloth

once a month.

7.2 Routine maintenance

7.2.1 Lubrication

Every 12 months the outer door hinges and the handle mechanism should be lightly lubricated using general-purpose oil or spray grease.

7.2.2 Defrosting

After an extended period of operation, defrosting may become necessary:

Risk of material damage

 Do not attempt to chip or scrape the ice with a sharp instrument. Allow the ice to melt

NOTICE!

naturally.

1. De-activate the alarm by switching the battery (alarm) switch (located behind the lockable

panel on the front of the freezer) to off (O).

2. Unplug the freezer from the mains/electrical supply.

3. Leave the innner and outer doors or lids open.

4. Allow the accumulated ice to melt.

5. Mop up the resulting water.

6. Dry and decontaminate the interior of the freezer.

7. When defrosting is complete, reconnect the freezer to the mains/electrical supply.

8. Turn the mains/power switch on (I) and re-activate the battery (alarm) switch.

7.2.3 Removing the inner doors (upright freezers except U725-G)

The inner doors of the freezer can be removed for defrosting and cleaning. To remove the inner doors of the upright freezers (except U725-G):

1. Fully open the outer door of the freezer.

2. Unscrew the door hinges.

HEF® and G -86 °C Freezers — Operating manual

3. Remove the inner door and set aside.

Abb. 10: Screw hinge inner door

Fig. 10: Screw hinge inner door

1 Inner door 2 Door hinge screw x4

Maintenance

Repeat procedure for each door.

7.2.4 Replacing the inner door (upright freezers except U725-G)

To reinstall the inner doors of the upright freezers (except U725-G):

1. Fully open the outer door of the freezer.

2. Reposition inner door in closed position.

3. Screw on door hinges.

4. If required, adjust by loosening the screws.

5. Close outer door.

7.2.5 Removing the inner doors (U725-G)

Maintenance

HEF® and G -86 °C Freezers — Operating manual

To remove the inner doors of the U725-G freezer:

1. Fully open the outer door of the Freezer.

2. Fully open the inner door.

3. Lift off inner door from hinges and set aside.

Abb. 11: Lift-off inner door

Fig. 11: Lift-off inner door

1 Inner door 2 Lift-off hinge

Repeat procedure for each door.

7.2.6 Replacing the inner door (U725-G)

To replace the inner door of the U725-G freezer:

1. Fully open the outer door of the freezer.

2. Fit door to hinge pins and close.

3. Check to ensure that inner door gasket is sealing against the freezer trim.

4. If required, adjust the latch retainer by loosening the screws and moving forward or

5. Close outer door.

backwards.

HEF® and G -86 °C Freezers — Operating manual

7.2.7 Electrical components

Risk of personal injury

 All electrical components that could cause possible ignition of refrigerant vapor during normal

WARNING!

operation have been enclosed in an IP65 enclosure.

 During routine maintenance, care must be taken to avoid any damage to the gaskets and

sealing grommets of these enclosures; also check the gaskets and sealing grommets routinely to ensure their integrity. Should any damage or deformity be detected, the gasket and/or sealing grommet must be replaced immediately.

 Failure to observe this safety warning will invalidate the warranty and could result in a

dangerous situation.

Lamps

Regularly check the indicator lamps:

 Press the ALARM TEST/MUTE key.

All of the indicator lamps should illuminate, and the display should read 8888.

Alarms

Regularly check the alarm:

 Press the ALARM TEST/MUTE key.

The TEMP indicator should illuminate and the audible alarm should sound.

Maintenance

NOTICE!

Hint!

Battery replacement

Risk of material damage

 There are no user controls behind any panels. The removal of any other part or panels from

the freezer by anyone other than a qualified, authorized Service Engineer may invalidate the warranty.

Risk of material damage

 Use only a replacement battery of the correct type and part number.

 The battery must be fitted so the terminals correspond to the polarity labels on the electrical

panel.

The YUASA–NP6 V 2.8 Ah battery is mounted on the electrical panel. This is located behind the right-hand base cover.

To replace the battery:

1. Switch off the mains/power switch and disconnect the mains/power supply.

2. Remove the side cover and the battery clamp securing the battery to the electrical panel.

3. Disconnect the battery terminals.

4. Install the new battery, fixing screws, and the side cover.

Be certain, when reconnecting the battery, to respect the correct polarity (red is + positive and black is – negative).

5. Reconnect the freezer to the mains/power supply and turn the mains/power switch on (I).

Fuses

Fuses must be replaced by a New Brunswick Scientific approved service engineer. Contact New Brunswick Service.

HEF® and G -86 °C Freezers — Operating manual

8 Troubleshooting

8.1 General errors

If you are experiencing a problem with your freezer, check the following troubleshooting guides before contacting your New Brunswick authorized Service technician.

8.2 Error messages

Troubleshooting

Symptom/ message

Door will not open

Cause Remedy

1. The door handle is locked.

2. The heated vent port is blocked.

1. Unlock the door handle.

2. Break up the ice in the vent port using the plunger, (see Heated vent port on p. 29).

If the door will not open:

 Call New Brunswick Service.

FILTER-CLEAN LED lights up

• Filter is contaminated.  Clean the filter, (see Air intake grille

and filter on p. 29).

If the LED remains lit:

 Call New Brunswick Service.

Your electronically-controlled New Brunswick freezer incorporates the unique Systems Monitoring And Reporting Technology (S.M.A.R.T. Plus™) self-diagnostic software to diagnose faults in its electronic systems, its probes and/or its refrigeration system.

This table interprets error codes that may appear in the control panel display:

Symptom/ message

E-01

E-02

E-03

E-04 Air-cooled condenser

Cause Remedy

• PT100 Probe 1 failure. This

probe, located inside the freezer cabinet, indicates cabinet temperature.

• Probe 2 failure. This probe

monitors the cascade condenser.

• This probe monitors the

air-cooled condenser.

temperature too high:

1. Filter may be blocked.



2. Ambient temperature may be too high.

If alarm continues to sound:

 Call New Brunswick Service department.



1. Clean filter according to the instructions (see Air intake grille and filter on p. 29).

2. Cool the room.



 Call New Brunswick Service department.

• Fan may have failed.

• Water supply not turned on,

insufficient flow, regulating valve not opening or defective (water-cooled version only).

A fan is required to cool the compressors on the water-cooled models.

Hint!

HEF® and G -86 °C Freezers — Operating manual

8.3 After a mains/power failure

If mains/power is interrupted, the POWER-FAIL indicator lamp (see Fig. 3 on p. 17), Item 3, will illuminate. In addition, the audible alarm will sound and the display will flash at approximately 10-second intervals.

When mains/power is restored, both alarm and light will automatically be cancelled.

If mains/power has been interrupted for only a short time, the internal temperature of the freezer will not have risen above the temperature setpoint (the user-set alarm threshhold), so normal operation will be resumed immediately.

If the interruption was long enough for the internal temperature to rise above the temperature setpoint, the TEMP-ALARM indicator will illuminate. If the internal temperature does not fall below the temperature setpoint within the programmed time after mains/power was restored, the audible alarm will sound again. The TEMP-ALARM indicator will extinguish when the internal temperature reaches the High Alarm temperature set point.

8.4 Interior warming

If the lid or door is left open long enough for the internal temperature to rise above the temperature setpoint, the same effects will be observed as described above regarding power failure.

To minimize the risk of this happening, the lid or door should only be opened when necessary, for a short period of time.

The upright freezers are fitted with internal doors which latch shut, minimizing temperature rise when the outer door is opened. Chest freezers are fitted with inner insulating lids to ensure efficient running of the freezer. The lids should remain fitted at all times when the freezer is running.

Troubleshooting

HEF® and G -86 °C Freezers — Operating manual

9 Technical data

9.0.1 Specifications

9.0.2 Specifications for U725-G Air-cooled and U725-G Water-cooled freezers

Part No. U9440-0005 U9440-0004

Internal Dimensions:

Height x Width x Depth

External Dimensions:

Height x Width x Depth

Capacity 725 Liters

Net Weight 315 kg

Lock Standard Standard

No. Compartments 33

Technical data

Interior Stainless steel grade 304L

Alarms High/Low temperature, power fail, battery low, filter clean, fault

Insulation Material Vacuum insulation panels and urethane foam

Remote alarm port Standard Standard

RS-485 interface Optional Optional

Refrigerants: High Stage Refrigerant: R290

‡Power Consumption:

• 230 V, 50 Hz electrical

Mains/Power Source and Current Rating:

230 V, 50 Hz 9.5 A 9.5 A

Pull Down Time: From +25 °C to -85 °C (freezer empty; 230 V, 50 Hz electrical supply)

Model No. U725-G Air-cooled U725-G Water-cooled

1365 x 865 x 615 mm

53.7 x 34.0 x 24.2 in

1950 x 1025 x 867 mm

76.8 x 40.4 x 34.1 in

25.6 cubic feet

693 lb

Low Stage Refrigerant: R170

635 Watts 596 Watts

supply

5.4 hours 4.6 hours

1365 x 865 x 615 mm

53.7 x 34.0 x 24.2 in

1950 x 1025 x 867 mm

76.8 x 40.4 x 34.1 in

725 Liters

25.6 cubic feet

317 kg

698 lb

Performance -50 °C to -86 °C at 32 °C maximum ambient operating temperature

Environmental Conditions All freezers use components tested to CE specifications listed below:

• Indoor use

• Altitude up to 2000 m

• Ambient temperature range 10 °C to 32 °C

• Maximum relative humidity 80 % for temperatures up to 31 °C, decreasing linearly to

50 % relative humidity at 40 °C

• Mains/power supply voltage fluctuations not to exceed ± 10 % of the nominal voltage

• Installation category II

• Pollution degree 2

‡ Freezer set to -80 °C, ambient 20 - 25 °C at rated mains/electrical supply

HEF® and G -86 °C Freezers — Operating manual

9.0.3 Specifications for U410 HEF and U570 HEF freezers

Model No. U410 HEF U570 HEF

Part No. U9260-0008 U9260-0007 U9270-0008 U9270-0007

Internal Dimensions:

Height x Width x Depth

External Dimensions:

Height x Width x Depth

Capacity 410 Liters

Net Weight 262 kg

Lock Standard Standard

No. Compartments 55

Interior Stainless steel grade 304L

Alarms High/Low temperature, power fail, battery low, filter clean, fault

Insulation Material Vacuum Insulation Panels and urethane foam

Remote alarm port Standard Standard

RS-485 interface Optional Optional

Refrigerants:

1265 x 550 x 575 mm

49.8 x 21.6 x 22.6 in

1915 x 800 x 852 mm

75.3 x 31.5 x 33.5 in

14.5 cubic feet

576 lb

1265 x 765 x 575 mm

49.8 x 30.1 x 22.6 in

1925 x 1025 x 852 mm

75.8 x 40.3 x 33.5 in

570 Liters

20.0 cubic feet

296 kg

651 lb

• 230 V, 50 Hz High Stage Refrigerant: R290

Low Stage Refrigerant: R170

• 120 V, 60 Hz High Stage Refrigerant: R404A

Low Stage Refrigerant: R508B

‡Power Consumption:

• 230 V, 50 Hz electrical

supply

• 120 V, 60 Hz electrical

supply

Mains/Power Source and Current Rating:

230 V, 50 Hz 5 A 6 A

120 V, 60 Hz 16.5 A 16.5 A

Pull Down Time: From +25 °C to -85 °C (freezer empty; 230 V, 50 Hz electrical supply)

Performance -50 °C to -86 °C at 32 °C maximum ambient operating temperature

Environmental Conditions All freezers use components tested to CE specifications listed below:

350 Watts 364 Watts

360 Watts 387 Watts

5.6 hours 5.5 hours

• Indoor use

• Altitude up to 2000 m

• Ambient temperature range 10 °C to 32 °C

• Maximum relative humidity 80 % for temperatures up to 31 °C, decreasing linearly to

50 % relative humidity at 40 °C

• Mains/power supply voltage fluctuations not to exceed ± 10 % of the nominal voltage

• Installation category II

• Pollution degree 2

Technical data

‡ Freezer set to -80 °C, ambient 20 - 25 °C at rated mains/electrical supply

HEF® and G -86 °C Freezers — Operating manual

9.0.4 Specifications for C660 HEF freezers

Model No. C660 HEF

Part No. U9250-0008 U9250-0009

Internal Dimensions:

Height x Width x Depth

External Dimensions:

Height x Width x Depth

Capacity 660 Liters

Net Weight 304 kg

Lock Standard

No. Compartments N/A

Interior Stainless steel grade 304L

Alarms High/Low temperature, power fail, battery low, filter clean, fault

Insulation Material Vacuum Insulation Panels and urethane foam

Remote alarm port Standard

RS-485 interface Optional

Refrigerants:

760 x 1470 x 590 mm

30 x 57.8 x 23.2 in

1075 x 2050 x 840 mm

42.3 x 80.7 x 33 in

23.3 cubic feet

669 lb

• 230 V, 50 Hz High Stage Refrigerant: R290

• 208 - 230 V, 60 Hz High Stage Refrigerant: R404A

‡Power Consumption:

• 230 V, 50 Hz electrical supply 475 Watts

Technical data

• 208 - 230 V, 60 Hz electrical supply 480 Watts

Mains/Power Source and Current Rating:

230 V, 50 Hz 6 A

208 - 230 V, 60 Hz 8 A

Pull Down Time: From +25 °C to -85 °C (freezer empty; 230 V, 50 Hz electrical supply)

Performance -50 °C to -86 °C at 32 °C maximum ambient operating temperature

Environmental Conditions All freezers are designed for:

Low Stage Refrigerant: R170

Low Stage Refrigerant: R508B

5.0 hours

• Indoor use

• Altitude up to 2000 m

• Ambient temperature range 10 °C to 32 °C

• Maximum relative humidity 80 % for temperatures up to 31 °C,

decreasing linearly to 50 % relative humidity at 40 °C

• Mains/power supply voltage fluctuations not to exceed ± 10 % of

the nominal voltage

• Installation category II

• Pollution degree 2

‡ Freezer set to -80 °C, ambient 20 - 25 °C at rated mains/electrical supply

HEF® and G -86 °C Freezers — Operating manual

10 Ordering Information

10.1 Accessories

A number of accessories are available for New Brunswick's range of ultra-low temperature freezers. Contact your local New Brunswick representative or distributor for details.

10.1.1 A2 independent temperature monitor

The A2 system is an independent temperature monitor with alarm, electronic chart recorder, and auto-dialer that communicates via the internet for remote monitoring from anywhere in the world. Ask your New Brunswick sales representative for availability.

10.1.2 Auto-dialers

Auto-dialers can call a set of preprogrammed telephone numbers in the event of an alarm condition and connect right into the freezer's remote alarm port.

10.1.3 Temperature probes

Additional Temperature Probes (such as the New Brunswick A2 monitoring system) can be installed upon request for an external alarm system or for validation.

10.1.4 Validation packages

Installation and operational qualifications are available.

10.1.5 Padlock adapter kits

Padlock adapter kits allow up to two user-supplied padlocks to be secured to outer door handle for extra security.

10.1.6 CO

10.1.7 Inventory racking systems

10.1.8 Chart recorder

and LN2 back-up systems

These systems are available to temporarily protect the contents of the freezer against the consequences of freezer failure or power failure. In an emergency, the system can inject either liquid carbon dioxide or liquid nitrogen from a storage bottle. Carbon dioxide back-up systems will maintain temperatures between -40 °C and -70 °C (subject to environmental conditions) for a period of up to 48 hours, during which time the freezer can be repaired. Liquid nitrogen back-up systems will maintain the freezer temperature at -86 °C.

and LN2 back up systems can be retrofitted by the user. Contact your local New Brunswick

distributor for options available. Instructions are included in the kit.

A very comprehensive set of anodized aluminium racks is available. These are designed to accommodate various sizes of boxes neatly, while giving maximum packing density in the freezer. Stainless-steel shelves and water proof boxes as well as custom racking are also available.

A chart recorder is available to provide a continuous record of the temperature inside the freezer over a period of seven days. The record is presented on a circular chart.

The following items are available for all freezer models:

Ordering Information

Order No. (International)

K0440-0355 Chart Recorder Kit 1

K0540-0025 Chart Recorder Paper 100

K0660-0051 Chart Recorder Pens 3

Description Quantity

HEF® and G -86 °C Freezers — Operating manual

10.1.9 New Brunswick BioCommand SFI datalogging software (RS-485 interface)

Ordering Information

New Brunswick Scientific's BioCommand® SFI Track and Trend software is available to trend and archive data from as many as 32 shakers, CO2 incubators, and/or freezers, simultaneously. This PC-based software works with any OPC-compliant equipment with an RS-232 or RS-485 port.

HEF® and G -86 °C Freezers — Operating manual

11 Transport, storage and disposal

11.1 Shut down

11.2 Transport

WARNING!

CAUTION!

11.3 Disposal



Switch the battery switch off before transporting or storing the equipment, (see Battery backup switch on p. 26).

Risk of personal injury

Lifting and transporting the freezer without suitable equipments can result in crushing and other injuries.

 Use a mechanical lifting equipment to load and unload the freezer.

Risk of material damage

Vibrations and impacts can cause the hermetically sealed compressors to slip out of their suspension system.

 Do not tilt the equipment.

 Avoid vibrations and impacts.

Perform the following steps if relocation is necessary:

1. Remove all shelves, racks, and boxes.

2. Carefully move the freezer.

In case the product is to be disposed of, the relevant legal regulations are to be observed.

Information on the disposal of electrical and electronic devices in the European Community:

Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, in the business-to-business sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. To document this, they have been marked with the following identification:

Transport, storage and disposal

Because disposal regulations may differ from one country to another within the EU, please contact your supplier if necessary.

In Germany, this is mandatory from March 23, 2006. From this date, the manufacturer has to offer a suitable method of return for all devices supplied after August 13, 2005. For all devices supplied before August 13, 2005, the last user is responsible for the correct disposal.

HEF® and G -86 °C Freezers — Operating manual

12 Certificates

DECLARATION OF CONFORMITY

New Brunswick Scientific declares that the Ultra Low Temperature Freezers products listed

conform to the European Union Directives and Standards identified in the declaration.

Certificates

Premium U410HEF Premium U570HEF Premium C660HEF Innova U725G Air Cooled Innova U725G Water Cooled

Marked Complies with EC directive 93/68/EEC

Electromagnetic Compatibility (EMC) EU Directive 2004/108/EC

Test Standard EMC EN61326-1 :2006

Low Voltage Directive (LVD) 2006/95/EC

Test Standard EN 61010-1 : First Edition

Test Standard EN 61010-1 : 2nd Edition (HEF Models)

UL 61010A-1 CSA C22.2 No. 1010.1 UL File E234047 (U.S. Voltage Models)

Conducted/Radiated Emissions FCC Part 15 Class B (U.S. Voltage Models)

Approved by _

M. King. Managing Director UK Operations

New Brunswick Scientific Co., Inc., 44 Talmadge Road, Edison, NJ 08818, USA New Brunswick Scientific (UK) Ltd, 17 Alban Park, St. Albans, AL4 0JJ, UK New Brunswick Scientific BV, Kerkenbos 1101, Nijmegen 6546 BC, NL

_________ on this 26th day of August, 2011

HEF® and G -86 °C Freezers — Operating manual

Index

Accessories .....................................................................39

Adjusting the shelf ...........................................................15

Air intake..........................................................................29

Alarm ...............................................................................26

Alarm activation ...............................................................22

Alarm test/mute key .........................................................26

Audible alarm.............................................................17, 26

Auto-dialers......................................................................39

Auto-reset ........................................................................12

Automatic reset................................................................12

Battery activation .............................................................22

Battery backup switch......................................................26

Battery replacement.........................................................33

C660 HEF Specifications.................................................38

Caution, explanation of ......................................................5

CFC ...................................................................................6

Chart recorder..................................................................39

Chest freezer, front view ....................................................9

Cleaning.....................................................................29, 30

Clearance ........................................................................13

CO2 back-up system........................................................39

Control panel ...................................................................17

Custom-made racks.........................................................39

Danger, explanation of.......................................................5

Defrosting ........................................................................30

Disposal ...........................................................................41

Door seal .........................................................................30

Door/lid stuck ...................................................................23

Electrical connection........................................................21

Electrical requirements ....................................................21

Error codes ......................................................................34

Extended warrenty option ................................................12

External monitoring..........................................................26

Factory-set temperature...................................................23

Fail, mains/power.............................................................35

Hazard symbols .................................................................5

HCFC................................................................................. 6

Ice in vent .................................................................. 23, 29

Indicator lights ................................................................. 11

Inner door, installing ........................................................31

Inner door, removing........................................................ 30

Inspection of boxes.......................................................... 10

Installing inner door ......................................................... 31

Intended use......................................................................7

Lid/door stuck ..................................................................23

LN2 back-up system ........................................................39

Lock key ..........................................................................23

Lockable panel ................................................................ 22

Mains/power failure.......................................................... 35

Mains/power supply......................................................... 21

Manual conventions...........................................................5

min.....................................................................................6

Monitoring.................................................................. 26, 40

Moving freezer................................................................. 41

Notice, explanation of ........................................................ 5

Packing list verification ....................................................10

Panel, removing...............................................................21

Part numbers................................................................... 39

Plunger ...................................................................... 23, 29

Power cord....................................................................... 21

Power fail ......................................................................... 23

Power failure....................................................................26

Power supply/mains......................................................... 21

Programming................................................................... 23

Racks............................................................................... 39

Recorder pens................................................................. 39

Recorder, chart ................................................................39

Registration, warranty......................................................12

Remote control .................................................................. 9

Remote monitoring ....................................................26, 40

Removing inner doors ..................................................... 30

Removing lockable panel ................................................ 21

Removing upright freezer lock barrel...............................15

Requirements, water supply ............................................ 14

Rise in temperature .........................................................35

rpm .................................................................................... 6

Index

HEF® and G -86 °C Freezers — Operating manual

RS-485 computer interface..............................................40

S.M.A.R.T. Plus..........................................................17, 34

Safety.................................................................................7

Set temp key ....................................................................23

Setpoint, temperature ......................................................35

Setting the operating temperature ...................................23

Shelf, adjusting ................................................................15

Specifications, C660 HEF................................................38

Specifications, U410 HEF................................................37

Specifications, U570 HEF................................................37

Specifications,U725-G .....................................................36

Symbols used ....................................................................5

Temp alarm......................................................................35

Temperature alarms.........................................................17

Temperature probes.........................................................39

Temperature setpoint.................................................23, 35

Testing alarm ...................................................................33

Testing alarm monitoring socket ......................................23

Testing indicator lamps ....................................................33

Trademarks........................................................................2

Troubleshooting ...............................................................34

Turning on the equipment ................................................21

U410 HEF Specifications.................................................37

U570 HEF Specifications.................................................37

U725-G Specifications .....................................................36

Unpacking boxes .............................................................10

Upright freezer, front view..................................................9

Upright freezer, side view ..................................................9

Using this manual ..............................................................5

Vacuum effect ..................................................................23

Validation packages.........................................................39

Vent............................................................................23, 29

Warming...........................................................................35

Warning, explanation of .....................................................5

Warranty ..........................................................................12

Warranty, extended ..........................................................12

Water cooled models .......................................................14

New Brunswick Scientiﬁc • www.nbsc.com • bioinfo@nbsc.com • For your local sales ofﬁce or distributor contact:

Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com

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Eppendorf North America, Inc. · Hauppauge, N.Y. USA · Tel: +1 516 334 7500 · +1 800 645 3050 · E-mail: info@eppendorf.com

Application Support Europe, International: Tel: +49 1803 666 789 · E-mail: support@eppendorf.com

North America: Tel: +1 800 645 3050 menu option 2 · E-mail: techserv@eppendorf.com

Asia Paciﬁc: Tel: +603 8023 6869 · E-mail: support_asiapaciﬁc@eppendorf.com

Eppendorf New Brunswick -86 °C Freezers G User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

1 Operating instructions

1.1 Using this manual

1.2 Danger symbols and danger levels

1.2.1 Hazard symbols

1.2.2 Degrees of danger

1.3 Symbols used

1.4 Abbreviations used

2 Safety

2.1 Intended use

2.2 Warnings for intended use

2.2.1 Manual conventions used

2.2.2 Health and safety at work act 1974

3 Product description

3.1 Main illustration

3.2 Delivery package

3.2.1 Inspection of boxes

3.2.2 Packing list verification

3.3 Product versions

3.3.1 Introduction

3.4 Features

3.5 Warranty

3.5.1 Warranty registration

3.5.2 Extended warranty option

4 Installation

4.1 Preparing installation

4.2 Selecting the location

4.2.1 Occupancy rating (230 V, 50 Hz models only)

4.2.2 Below ground installations (230 V, 50 Hz models only)

4.2.3 Installation categories (230 V, 50 Hz models only)

4.3 Requirements for water-cooled models

4.4 Installing the shelves

4.5 Lockable freezer handle

5 Operating controls and function

5.1 Controls and function

6 Operation

6.1 Getting started

6.1.1 Plug in

6.1.2 Turning the freezer On/Off

6.1.3 Alarm/battery activation

6.1.4 Testing the alarm monitoring socket

6.1.5 Vacuum effect

6.2 Programming the freezer

6.2.1 Setting operating temperature

6.2.2 Setting high alarm setpoint

6.2.3 Setting low alarm setpoint

6.2.4 Checking temperature and alarm setpoint settings

6.2.5 Setting the alarm delay

6.2.6 Changing lock codes

6.2.7 Setting the temperature offset

6.3 Battery backup switch

6.4 Alarm monitoring socket

7 Maintenance

7.1 Cleaning

7.1.1 Painted surfaces

7.1.2 Panels and shelves

7.1.3 Air intake grille and filter

7.1.4 Heated vent port

7.1.5 Door or lid seal

7.2 Routine maintenance

7.2.1 Lubrication

7.2.2 Defrosting

7.2.3 Removing the inner doors (upright freezers except U725-G)

7.2.4 Replacing the inner door (upright freezers except U725-G)

7.2.5 Removing the inner doors (U725-G)

7.2.6 Replacing the inner door (U725-G)

7.2.7 Electrical components

8 Troubleshooting

8.1 General errors

8.2 Error messages

8.3 After a mains/power failure

8.4 Interior warming

9 Technical data

9.0.1 Specifications

9.0.2 Specifications for U725-G Air-cooled and U725-G Water-cooled freezers

9.0.3 Specifications for U410 HEF and U570 HEF freezers

9.0.4 Specifications for C660 HEF freezers