Eppendorf New Brunswick -86 °C Freezers G User Manual

Operating manual
HEF® and G -86 C Freezers
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erating manua
September 16, 2011
New Brunswick -86 °C Freezers
HEF® and G
Operating manual
M1288-0058
Revision H
Copyright
Copyright© 2011 New Brunswick Scientific Co., Inc., USA. No part of this publication may be reproduced without the prior permission of the copyright owner.
New Brunswick Scientific reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.
Trademarks
Eppendorf® is a registered trademark of Eppendorf AG, Germany.
BioCommand
®
is a registered trademark of New Brunswick Scientific Co., Inc., USA.
Innova® is a registered trademark of New Brunswick Scientific Co., Inc., USA.
HEF® is a registered trademark of New Brunswick Scientific, Co., Inc., USA.
New Brunswick™, and the New Brunswick Logo™ are trademarks of Eppendorf AG, Germany.
S.M.A.R.T. Plus™ is a pending trademark of New Brunswick Scientific Co., Inc., USA.
Trademarks are not marked in all cases with ™ or
®
in this manual.
New Brunswick Scientific has attempted to identify the ownership of all trademarks from public records. Any omissions or errors are unintentional.
September 19, 2011 Revision H M1288-0058
HEF® and G -86 °C Freezers — Operating manual
Table of contents
1 Table of contents
1
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2.1 Manual conventions used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2.2 Health and safety at work act 1974. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.2.1 Inspection of boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.2.2 Packing list verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.3 Product versions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.4 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.5 Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.5.1 Warranty registration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.5.2 Extended warranty option . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Table of contents
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.2.1 Occupancy rating (230 V, 50 Hz models only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.2.2 Below ground installations (230 V, 50 Hz models only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.2.3 Installation categories (230 V, 50 Hz models only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.3 Requirements for water-cooled models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.4 Installing the shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.5 Lockable freezer handle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5 Operating controls and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1 Controls and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.1 Getting started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.1.1 Plug in . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.1.2 Turning the freezer On/Off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.1.3 Alarm/battery activation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.1.4 Testing the alarm monitoring socket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
6.1.5 Vacuum effect . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
6.2 Programming the freezer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
6.2.1 Setting operating temperature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
6.2.2 Setting high alarm setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.2.3 Setting low alarm setpoint. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.2.4 Checking temperature and alarm setpoint settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
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Table of contents
HEF® and G -86 °C Freezers — Operating manual
6.2.5 Setting the alarm delay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.2.6 Changing lock codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.2.7 Setting the temperature offset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.3 Battery backup switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.4 Alarm monitoring socket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
7.1.1 Painted surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
7.1.2 Panels and shelves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
7.1.3 Air intake grille and filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
7.1.4 Heated vent port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
7.1.5 Door or lid seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
7.2 Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
7.2.1 Lubrication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
7.2.2 Defrosting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
7.2.3 Removing the inner doors (upright freezers except U725-G) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
7.2.4 Replacing the inner door (upright freezers except U725-G) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.2.5 Removing the inner doors (U725-G). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
7.2.6 Replacing the inner door (U725-G). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
7.2.7 Electrical components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
8.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
8.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
8.3 After a mains/power failure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
8.4 Interior warming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
10 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10.1.1 A2 independent temperature monitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10.1.2 Auto-dialers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10.1.3 Temperature probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10.1.4 Validation packages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10.1.5 Padlock adapter kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10.1.6 CO
10.1.7 Inventory racking systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10.1.8 Chart recorder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10.1.9 New Brunswick BioCommand SFI datalogging software (RS-485 interface) . . . . . . . . . . . . . . . . . . 40
11 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
11.1 Shut down. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
11.2 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
11.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
and LN2 back-up systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
2
12 Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
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HEF® and G -86 °C Freezers — Operating manual

1 Operating instructions

1 Operating instructions

1.1 Using this manual

Carefully read this operating manual before using the device for the first time.
Also observe the operating manual enclosed with the accessories.
The operating manual should be considered as part of the product and stored in a location
that is easily accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our
website http://www.nbsc.com

1.2 Danger symbols and danger levels

1.2.1 Hazard symbols

Hazard point Burns
Electric shock Material damage
.
1
Operating instructions
Crush

1.2.2 Degrees of danger

The following degree levels are used in safety messages throughout this manual. Acquaint yourself with each item and the potential risk if you disregard the safety message.
DANGER Will lead to severe injuries or death.
WARNIN G May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.

1.3 Symbols used

Example Meaning
You are requested to perform an action.
1.
2.
Perform these actions in the sequence described.
List.
References useful information.
5
1
Operating instructions
HEF® and G -86 °C Freezers — Operating manual

1.4 Abbreviations used

A Amp
CFC Chlorofluorocarbons
°C Degree Celsius
G Green
HEF High Efficiency
HCFC Hydrochlorofluorocarbon
Hz Hertz
kg Kilogram
lb Pound
m Meter
min Minute
mm Millimeter
N/A Not applicable
rpm Revolutions per Minute (min
ULT Ultra-Low Temperature
V Volt
-1
)
6
HEF® and G -86 °C Freezers — Operating manual

2 Safety

2Safety

2.1 Intended use

New Brunswick HEF and G (Green) freezers are designed to provide precise, ultra-low temperature environments for cold storage of scientific or medical materials. They are designed to provide ultra-low temperature sample storage from -50 °C to -86 °C at 32 °C maximum ambient operating temperature.

2.2 Warnings for intended use

2.2.1 Manual conventions used

Risk of material damage
This equipment must be operated as described in this manual.
NOTICE!
Please read the entire operating manual before attempting to use this equipment. If
operational guidelines are not followed, equipment damage may occur.
Risk of personal injury
Do not use this equiment in a hazardous atmosphere or with hazardous materials for which
CAUTION!
the equipment was not designed.
Please read the entire operating manual before attempting to use this equipment. If
operational guidelines are not followed, personal injury may occur.
Risk of personal injury
Crush Warning messages alert you to specific procedures or practices regarding heavy
CAUTION!
objects which, if not followed correctly, could result in serious personal injury.
Risk of personal injury
Flammable warning messages alert you to possible risks of of personal injury and equipment
WARNING!
damage: protect the system from sparks and flames.
2
Safety

2.2.2 Health and safety at work act 1974

(FOR THE UNITED KINGDOM)
New Brunswick Scientific, as manufacturers and suppliers of laboratory equipment, are obliged under the terms of the above Act to provide our users with instructions on the safe installation, operation and maintenance of our equipment.
Our equipment is designed to acceptable standards and does not entail any hazard if used, as advised in the attached instructions.
The following safety precautions should be observed by all personnel using this equipment:
1. Read and understand this manual. If in doubt, contact your local New Brunswick sales office.
2. Do not remove any covers. There are no operable controls other than those referred to in this manual. There are voltages in excess of 41.5 volts AC behind the covers.
3. Use freezer gloves at all times when loading or unloading the equipment. The temperature of operation is such that direct contact with the cold contents or inside the equipment can burn unprotected skin.
4. Observe good housekeeping practices, at all times keeping the equipment and the adjacent areas clean, dry and uncluttered.
5. Should any malfunctions occur or be suspected, immediately call a qualified service engineer to investigate.
7
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HEF® and G -86 °C Freezers — Operating manual
6. The hydrocarbon (Group A3) refrigerants used in these freezers are flammable and therefore appropriate attention must be paid to avoid leaks and to keep the freezer away from sparks and flames.
Any person involved with working on or entering the refrigeration circuit should hold a current and valid certificate from an industry-accredited assessment authority which authorizes his/her competence to handle refrigerants (including hydrocarbons) safely in accordance with local regulations and legislation.
Safety
8
HEF® and G -86 °C Freezers — Operating manual

3 Product description

3 Product description

3.1 Main illustration

Abb. 1: Upright freezer - Side an d front views
12 3
3
Product description
4
5
76891011
Fig. 1: Upright freezer - Side and front views
1 Heated vent port 2 Door handle (lockable)
3 Control panel/display 4 Model label
5 Battery switch behind lockable panel 6 On/Off circuit breaker behind lockable panel
7 Transport castors 8 Chart recorder (optional)
9 Air filter grille 10 Quarter turn fastener
11 Specification plate
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HEF® and G -86 °C Freezers — Operating manual
3
Abb. 2: Chest freezer - Front view
23
1
4 5 6
7
8
9
Fig. 2: Chest freezer - Front view
1 Heated vent port (inside compressor housing) 2 Lockable handle
3 Control panel/display 4 Battery switch (alarm) behind lockable panel
5 On/Off circuit breaker behind lockable panel 6 Chart recorder (optional)
Product description
7 Quarter turn fasteners 8 Air filter grille
9 Braked castors (front)

3.2 Delivery package

3.2.1 Inspection of boxes

Inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage to the carrier and to your local New Brunswick Sales Order Department immediately.

3.2.2 Packing list verification

Unpack your order, saving the packing materials for possible future use. Save the operating manual for instruction and reference. Verify against your New Brunswick packing list that you have received the correct materials, and that nothing is missing. If any part of your order was damaged during shipping, is missing, or fails to operate, fill out the "Customer Feedback" form,
.
NOTICE!
available online at www.nbsc.com/CustomerFeedback.aspx
Risk of material damage
Vacuum insulation panels are used in the construction of Innova U725G and U410 HEF,
U570 HEF, and C660 HEF model freezers. Inspect the cabinet panels for punctures or other damage that compromises the integrity of the product.
These panels are mounted in the cavity against the steel outer wall of the freezer. Any drilling
or puncture to the outer wall could release the vacuum from the panel, resulting in impaired freezer performance.
Any unauthorized punctures or other damage deliberately made to the cabinet walls will
invalidate the warranty.
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HEF® and G -86 °C Freezers — Operating manual

3.3 Product versions

3.3.1 Introduction

This manual provides the user with the necessary information for installation and operation of New Brunswick’s energy saving HEF and G range of Ultra-Low Temperature freezers: Innova U725G, and the HEF models. It also provides some preliminary user maintenance information.
This manual covers the following freezer models:
Model (230 V, 50 Hz) Capacity
Innova U725-G Air-cooled 725 liters (25.6 cubic feet)
Innova U725-G Water-cooled 725 liters (25.6 cubic feet)
U410 HEF 410 liters (14.5 cubic feet)
U570 HEF 570 liters (20.1 cubic feet)
C660 HEF 660 liters (23.3 cubic feet)
®
3
All the above mentioned HEF and G freezers are totally free of CFCs (Chlorofluorocarbons) and HCFCs (Hydrochlorofluorocarbons). They use HCs (Hydrocarbons) as refrigerants.
The use of Hydrocarbons as refrigerant is prohibited in the United States.
Hint!
The following HEF freezer models utilize HFCs (Hydrofluorocarbons) as refrigerants, and are offered for the United States market.
Model (120 V, 60 Hz) Capacity
U410 HEF 410 liters (14.5 cubic feet)
U570 HEF 570 liters (20.1 cubic feet)
Product description

3.4 Features

Model (208 - 230 V, 60 Hz) Capacity
C660 HEF 660 liters (23.3 cubic feet)
The freezers are manufactured using high quality steel and electronics for long life. The HEF and G-Series freezers have many features designed to provide ease of use and maintanence, security and reliability to your ultra-low temperature storage needs. They are built to stringent regulatory requirements for safety and environmental friendliness and disposability, and they are CE and UL certified.
Features include:
Designed to be exceptionally energy efficient and eco-friendly.
A setpoint keypad and digital temperature readout are provided on a control panel, located on
the door of the upright freezers and on the console on the right side of chest freezers. Controls for setting the freezer temperature and alarm warning setpoints are enabled by an electronic lock. A unique code, which is selected by the user through the keypad, sets this lock. The code can be changed at any time, provided the current setting of the lock is known.
On delivery, the lock code is "0000" (unlocked).
Indicator lamps on the control panel provide warnings of power loss, system failure,
exceeding High/Low temperature setpoints, low battery voltage, and filter blockage. There is also an LED to indicate when the freezer is under remote control via the RS-485 optional computer interface.
Heavy-duty commercially available compressors provide rapid temperature pull-down and
recovery after door opening.
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HEF® and G -86 °C Freezers — Operating manual
A heated port with ice-clearing plunger prevents vacuum formation enabling the outer door to
be easily opened at anytime.
All interior panels and shelves are made of high grade corrosion-resistant stainless steel,
making them durable and easy to keep clean and sterilize.
Heavy duty castors enable easy installation and relocation.
Two access ports allow easy addition of sensors, or of back-up systems that can provide
cooling protection for your samples in the case of a power outage or other system failure.
An alarm contact is provided for connection to an external monitoring device or system.
The freezers can be fitted with an optional 7-day circular chart recorder to provide
independent temperature recording.
An automatic reset restarts freezers at random 15 second intervals to protect the
microprocessor controller from damage by electrical spikes caused by multiple freezers turning on at once.
Lockable freezer handles on outer door provide added security against unauthorized users.
Multiple accessories are offered, including CO
systems, external voltage stabilizer, inventory racking, and more.

3.5 Warranty

New Brunswick Scientific equipment is protected by a comprehensive warranty. The warranty covers faulty components and assembly, and our obligation under this warranty is limited to repairing or replacing the instrument or part thereof, which shall prove to be defective after our examination.
The warranty does not cover loss of time or materials, such as the loss of biological or biochemical by-products caused by any work interruption resulting from equipment failure; it does not extend to equipment that has been subject to misuse, neglect, accident or improper installation or application; nor does it cover any machine that has been repaired or altered by anyone other than an authorized factory-trained service representative, without prior written
Product description
approval from your local New Brunswick sales office or distributor.
Expendable items such as bearings and seals, lamps, probes, sensors, glass, filters, single-use vessels, etc., are not covered.
The warranty begins on the date the equipment ships from New Brunswick Scientific or an authorized distributor and extends through the period indicated in the chart below:
/LN2 back-up systems, remote monitoring
2

3.5.1 Warranty registration

3.5.2 Extended warranty option

12
Instrument Parts Warranty Labor Warranty
Freezers ULT Freezers 5 years; vacuum
insulation panels: 12 years
1
Accessories
1
Chart recorders, CO2/LN2 back-up systems, etc.
To register your warranty, complete the online form at www.nbsc.com Help? tab.
A variety of service plans are offered to help minimize downtime from unexpected malfunctions in equipment operation. Speak to your New Brunswick sales representative for more information.
1 year 1 year
2 years
under the How Can We
HEF® and G -86 °C Freezers — Operating manual

4 Installation

4 Installation

4.1 Preparing installation

Risk of personal injury
DO NOT attempt to lift any freezer by hand. Preferred lifting for loading and unloading is by
WARNING!
NOTICE!

4.2 Selecting the location

mechanical lifting equipment.
Risk of material damage
Maintenance, adjustment and repair work should be carried out only by QUALIFIED,
EXPERIENCED personnel who have been AUTHORIZED to undertake such work by New Brunswick Scientific or its authorized agents.
Failure to use authorized service agents will invalidate the warranty.
All freezers are mounted on castors for ease of movement. U725-G model freezers have feet that provide both a leveling feature and a locking feature to stop the freezer from rolling once it is in place. HEF upright freezers and chest freezers have locking front castors; locking front castors do not provide leveling adjustment, so the site chosen for the freezer must have a flat, level floor.
Position the freezer to allow disconnection to be made in respect to removal of the plug or appliance coupler, also the free air entry through the intake grille in the front and free air exit from the back. Provide a clearance of at least 150 mm (6 in) on all sides.
For efficient temperature control, the freezer should be placed in a shaded area, away from sources of excessive heat. For maximum cooling capability, the product should be located in an air-conditioned room.
4
Installation

4.2.1 Occupancy rating (230 V, 50 Hz models only)

This equipment has a category A1 - B1 occupancy rating, with a refrigerant charge of less than
0.15 kg per sealed system.
Systems charged with less than 0.15 kg may be installed in any size room as long as adequate ventilation is provided, in order to remove rejected heat from the freezer and to vent any sudden loss of refrigerant in case of system failure.

4.2.2 Below ground installations (230 V, 50 Hz models only)

We recommend allowing at least 18 m refrigerant concentration from exceeding 20 % of the Lower Flammability Limit (LFL) in the event of a sudden loss of refrigerant into the room.
Basements and cellars must have adequate ventilation for the removal of heat rejected from the freezer(s).
3
(23.5 yd3) room volume for each freezer, to keep the air/
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HEF® and G -86 °C Freezers — Operating manual

4.2.3 Installation categories (230 V, 50 Hz models only)

Category Examples Requirements
4
A1 (domestic/ public) Hospitals
B1 (commercial/ private) Business or professional

4.3 Requirements for water-cooled models

If your freezer has a water-cooled condenser, the following are water supply and drainage requirements:
Minimum flow requirements
Max Inlet pressure 10 bars
Min Inlet pressure 1 bar
Prisons
Theatres
Schools
Supermarkets
Hotels
Dwellings
offices
Shops
Restaurants
Laboratories
General manufacturing
3.8 liters/minute
< 0.15 kg refrigerant per sealed system
< 0.15 kg refrigerant per sealed system
Installation
Maximum supply
temperature
Minimum supply
temperature
Connection size Inlet : 15 mm x ½” BSPT
Water quality Water must be clean and free from particles that could cause
Drainage requirements
Typical Flow Rates At a setpoint of -85 °C, an ambient temperature of 25 °C, and a
1
Water consumption will increase as water temperature increases.
2
The condenser must never be allowed to freeze during operation. If, during normal cycling,
water temperature approaches 6.0 °C, this must be checked.
3
This installation requires checking the high stage discharge pressure and may require an adjustment of the water regulating valve; both operations MUST be carried out by a qualified engineer.
1
2
25 °C
7.0 °C
Outlet: 15 mm x ½” BSPT
blockage in the regulating valve or heat exchanger. A suitable inline strainer must be placed in the inlet pipe if there is any doubt about the cleanliness of the supply. (Minimum filter requirement is 60 mesh 0.25 mm aperture).
3
A recirculated cooler return line and a main supply line to the waste drain are required.
water inlet temperature of 20 °C:
47 L/hr for the U725-G.
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