New Brunswick Scientific reserves the right to change information in this document without notice. Updates to information
in this document reflect our commitment to continuing product development and improvement.
Trademarks
Eppendorf® is a registered trademark of Eppendorf AG, Germany.
BioCommand
®
is a registered trademark of New Brunswick Scientific Co., Inc., USA.
Innova® is a registered trademark of New Brunswick Scientific Co., Inc., USA.
HEF® is a registered trademark of New Brunswick Scientific, Co., Inc., USA.
New Brunswick™, and the New Brunswick Logo™ are trademarks of Eppendorf AG, Germany.
S.M.A.R.T. Plus™ is a pending trademark of New Brunswick Scientific Co., Inc., USA.
Trademarks are not marked in all cases with ™ or
®
in this manual.
New Brunswick Scientific has attempted to identify the ownership of all trademarks from public records. Any omissions or
errors are unintentional.
Carefully read this operating manual before using the device for the first time.
Also observe the operating manual enclosed with the accessories.
The operating manual should be considered as part of the product and stored in a location
that is easily accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our
website http://www.nbsc.com
1.2Danger symbols and danger levels
1.2.1Hazard symbols
Hazard pointBurns
Electric shockMaterial damage
.
1
Operating instructions
Crush
1.2.2Degrees of danger
The following degree levels are used in safety messages throughout this manual. Acquaint
yourself with each item and the potential risk if you disregard the safety message.
DANGERWill lead to severe injuries or death.
WARNIN GMay lead to severe injuries or death.
CAUTIONMay lead to light to moderate injuries.
NOTICEMay lead to material damage.
1.3Symbols used
ExampleMeaning
You are requested to perform an action.
1.
2.
Perform these actions in the sequence described.
• List.
References useful information.
5
1
Operating instructions
HEF® and G -86 °C Freezers — Operating manual
1.4Abbreviations used
AAmp
CFCChlorofluorocarbons
°CDegree Celsius
GGreen
HEFHigh Efficiency
HCFCHydrochlorofluorocarbon
HzHertz
kgKilogram
lbPound
mMeter
minMinute
mmMillimeter
N/ANot applicable
rpmRevolutions per Minute (min
ULTUltra-Low Temperature
VVolt
-1
)
6
HEF® and G -86 °C Freezers — Operating manual
2Safety
2Safety
2.1Intended use
New Brunswick HEF and G (Green) freezers are designed to provide precise, ultra-low
temperature environments for cold storage of scientific or medical materials. They are designed
to provide ultra-low temperature sample storage from -50 °C to -86 °C at 32 °C maximum
ambient operating temperature.
2.2Warnings for intended use
2.2.1Manual conventions used
Risk of material damage
This equipment must be operated as described in this manual.
NOTICE!
Please read the entire operating manual before attempting to use this equipment. If
operational guidelines are not followed, equipment damage may occur.
Risk of personal injury
Do not use this equiment in a hazardous atmosphere or with hazardous materials for which
CAUTION!
the equipment was not designed.
Please read the entire operating manual before attempting to use this equipment. If
operational guidelines are not followed, personal injury may occur.
Risk of personal injury
Crush Warning messages alert you to specific procedures or practices regarding heavy
CAUTION!
objects which, if not followed correctly, could result in serious personal injury.
Risk of personal injury
Flammable warning messages alert you to possible risks of of personal injury and equipment
WARNING!
damage: protect the system from sparks and flames.
2
Safety
2.2.2Health and safety at work act 1974
(FOR THE UNITED KINGDOM)
New Brunswick Scientific, as manufacturers and suppliers of laboratory equipment, are obliged
under the terms of the above Act to provide our users with instructions on the safe installation,
operation and maintenance of our equipment.
Our equipment is designed to acceptable standards and does not entail any hazard if used, as
advised in the attached instructions.
The following safety precautions should be observed by all personnel using this equipment:
1. Read and understand this manual. If in doubt, contact your local New Brunswick sales office.
2. Do not remove any covers. There are no operable controls other than those referred to in this
manual. There are voltages in excess of 41.5 volts AC behind the covers.
3. Use freezer gloves at all times when loading or unloading the equipment. The temperature of
operation is such that direct contact with the cold contents or inside the equipment can burn
unprotected skin.
4. Observe good housekeeping practices, at all times keeping the equipment and the adjacent
areas clean, dry and uncluttered.
5. Should any malfunctions occur or be suspected, immediately call a qualified service engineer
to investigate.
7
2
HEF® and G -86 °C Freezers — Operating manual
6. The hydrocarbon (Group A3) refrigerants used in these freezers are flammable and therefore
appropriate attention must be paid to avoid leaks and to keep the freezer away from sparks
and flames.
Any person involved with working on or entering the refrigeration circuit should hold a current and
valid certificate from an industry-accredited assessment authority which authorizes his/her
competence to handle refrigerants (including hydrocarbons) safely in accordance with local
regulations and legislation.
Inspect the boxes carefully for any damage that may have occurred during shipping. Report any
damage to the carrier and to your local New Brunswick Sales Order Department immediately.
3.2.2Packing list verification
Unpack your order, saving the packing materials for possible future use. Save the operating
manual for instruction and reference. Verify against your New Brunswick packing list that you
have received the correct materials, and that nothing is missing. If any part of your order was
damaged during shipping, is missing, or fails to operate, fill out the "Customer Feedback" form,
.
NOTICE!
available online at www.nbsc.com/CustomerFeedback.aspx
Risk of material damage
Vacuum insulation panels are used in the construction of Innova U725G and U410 HEF,
U570 HEF, and C660 HEF model freezers. Inspect the cabinet panels for punctures or other
damage that compromises the integrity of the product.
These panels are mounted in the cavity against the steel outer wall of the freezer. Any drilling
or puncture to the outer wall could release the vacuum from the panel, resulting in impaired
freezer performance.
Any unauthorized punctures or other damage deliberately made to the cabinet walls will
invalidate the warranty.
10
HEF® and G -86 °C Freezers — Operating manual
3.3Product versions
3.3.1Introduction
This manual provides the user with the necessary information for installation and operation of
New Brunswick’s energy saving HEF and G range of Ultra-Low Temperature freezers: Innova
U725G, and the HEF models. It also provides some preliminary user maintenance information.
All the above mentioned HEF and G freezers are totally free of CFCs (Chlorofluorocarbons) and
HCFCs (Hydrochlorofluorocarbons). They use HCs (Hydrocarbons) as refrigerants.
The use of Hydrocarbons as refrigerant is prohibited in the United States.
Hint!
The following HEF freezer models utilize HFCs (Hydrofluorocarbons) as refrigerants, and are
offered for the United States market.
Model (120 V, 60 Hz)Capacity
U410 HEF410 liters (14.5 cubic feet)
U570 HEF570 liters (20.1 cubic feet)
Product description
3.4Features
Model (208 - 230 V, 60 Hz)Capacity
C660 HEF660 liters (23.3 cubic feet)
The freezers are manufactured using high quality steel and electronics for long life. The HEF and
G-Series freezers have many features designed to provide ease of use and maintanence,
security and reliability to your ultra-low temperature storage needs. They are built to stringent
regulatory requirements for safety and environmental friendliness and disposability, and they are
CE and UL certified.
Features include:
• Designed to be exceptionally energy efficient and eco-friendly.
• A setpoint keypad and digital temperature readout are provided on a control panel, located on
the door of the upright freezers and on the console on the right side of chest freezers.
Controls for setting the freezer temperature and alarm warning setpoints are enabled by an
electronic lock. A unique code, which is selected by the user through the keypad, sets this
lock. The code can be changed at any time, provided the current setting of the lock is known.
On delivery, the lock code is "0000" (unlocked).
• Indicator lamps on the control panel provide warnings of power loss, system failure,
exceeding High/Low temperature setpoints, low battery voltage, and filter blockage. There is
also an LED to indicate when the freezer is under remote control via the RS-485 optional
computer interface.
• Heavy-duty commercially available compressors provide rapid temperature pull-down and
recovery after door opening.
11
3
HEF® and G -86 °C Freezers — Operating manual
• A heated port with ice-clearing plunger prevents vacuum formation enabling the outer door to
be easily opened at anytime.
• All interior panels and shelves are made of high grade corrosion-resistant stainless steel,
making them durable and easy to keep clean and sterilize.
• Heavy duty castors enable easy installation and relocation.
• Two access ports allow easy addition of sensors, or of back-up systems that can provide
cooling protection for your samples in the case of a power outage or other system failure.
• An alarm contact is provided for connection to an external monitoring device or system.
• The freezers can be fitted with an optional 7-day circular chart recorder to provide
independent temperature recording.
• An automatic reset restarts freezers at random 15 second intervals to protect the
microprocessor controller from damage by electrical spikes caused by multiple freezers
turning on at once.
• Lockable freezer handles on outer door provide added security against unauthorized users.
• Multiple accessories are offered, including CO
systems, external voltage stabilizer, inventory racking, and more.
3.5Warranty
New Brunswick Scientific equipment is protected by a comprehensive warranty. The warranty
covers faulty components and assembly, and our obligation under this warranty is limited to
repairing or replacing the instrument or part thereof, which shall prove to be defective after our
examination.
The warranty does not cover loss of time or materials, such as the loss of biological or
biochemical by-products caused by any work interruption resulting from equipment failure; it does
not extend to equipment that has been subject to misuse, neglect, accident or improper
installation or application; nor does it cover any machine that has been repaired or altered by
anyone other than an authorized factory-trained service representative, without prior written
Product description
approval from your local New Brunswick sales office or distributor.
Expendable items such as bearings and seals, lamps, probes, sensors, glass, filters, single-use
vessels, etc., are not covered.
The warranty begins on the date the equipment ships from New Brunswick Scientific or an
authorized distributor and extends through the period indicated in the chart below:
/LN2 back-up systems, remote monitoring
2
3.5.1Warranty registration
3.5.2Extended warranty option
12
InstrumentParts WarrantyLabor Warranty
FreezersULT Freezers5 years; vacuum
insulation panels: 12
years
1
Accessories
1
Chart recorders, CO2/LN2 back-up systems, etc.
To register your warranty, complete the online form at www.nbsc.com
Help? tab.
A variety of service plans are offered to help minimize downtime from unexpected malfunctions in
equipment operation. Speak to your New Brunswick sales representative for more information.
1 year1 year
2 years
under the How Can We
HEF® and G -86 °C Freezers — Operating manual
4Installation
4Installation
4.1Preparing installation
Risk of personal injury
DO NOT attempt to lift any freezer by hand. Preferred lifting for loading and unloading is by
WARNING!
NOTICE!
4.2Selecting the location
mechanical lifting equipment.
Risk of material damage
Maintenance, adjustment and repair work should be carried out only by QUALIFIED,
EXPERIENCED personnel who have been AUTHORIZED to undertake such work by New
Brunswick Scientific or its authorized agents.
Failure to use authorized service agents will invalidate the warranty.
All freezers are mounted on castors for ease of movement. U725-G model freezers have feet that
provide both a leveling feature and a locking feature to stop the freezer from rolling once it is in
place. HEF upright freezers and chest freezers have locking front castors; locking front castors
do not provide leveling adjustment, so the site chosen for the freezer must have a flat, level floor.
Position the freezer to allow disconnection to be made in respect to removal of the plug or
appliance coupler, also the free air entry through the intake grille in the front and free air exit from
the back. Provide a clearance of at least 150 mm (6 in) on all sides.
For efficient temperature control, the freezer should be placed in a shaded area, away from
sources of excessive heat. For maximum cooling capability, the product should be located in an
air-conditioned room.
4
Installation
4.2.1Occupancy rating (230 V, 50 Hz models only)
This equipment has a category A1 - B1 occupancy rating, with a refrigerant charge of less than
0.15 kg per sealed system.
Systems charged with less than 0.15 kg may be installed in any size room as long as adequate
ventilation is provided, in order to remove rejected heat from the freezer and to vent any sudden
loss of refrigerant in case of system failure.
We recommend allowing at least 18 m
refrigerant concentration from exceeding 20 % of the Lower Flammability Limit (LFL) in the event
of a sudden loss of refrigerant into the room.
Basements and cellars must have adequate ventilation for the removal of heat rejected from the
freezer(s).
3
(23.5 yd3) room volume for each freezer, to keep the air/
If your freezer has a water-cooled condenser, the following are water supply and drainage
requirements:
Minimum flow
requirements
Max Inlet pressure10 bars
Min Inlet pressure1 bar
Prisons
Theatres
Schools
Supermarkets
Hotels
Dwellings
offices
Shops
Restaurants
Laboratories
General manufacturing
3.8 liters/minute
< 0.15 kg refrigerant per
sealed system
< 0.15 kg refrigerant per
sealed system
Installation
Maximum supply
temperature
Minimum supply
temperature
Connection sizeInlet : 15 mm x ½” BSPT
Water qualityWater must be clean and free from particles that could cause
Drainage requirements
Typical Flow Rates At a setpoint of -85 °C, an ambient temperature of 25 °C, and a
1
Water consumption will increase as water temperature increases.
2
The condenser must never be allowed to freeze during operation. If, during normal cycling,
water temperature approaches 6.0 °C, this must be checked.
3
This installation requires checking the high stage discharge pressure and may require an
adjustment of the water regulating valve; both operations MUST be carried out by a qualified
engineer.
1
2
25 °C
7.0 °C
Outlet: 15 mm x ½” BSPT
blockage in the regulating valve or heat exchanger. A suitable
inline strainer must be placed in the inlet pipe if there is any doubt
about the cleanliness of the supply. (Minimum filter requirement is
60 mesh 0.25 mm aperture).
3
A recirculated cooler return line and a main supply line to the
waste drain are required.
water inlet temperature of 20 °C:
47 L/hr for the U725-G.
14
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