Metrohm Inline Dialysis User Manual

IC equipment

IC equipment: Dialysis (6.5330.100)

Manual

8.110.8028EN / 2014-02-20

Metrohm AG
CH-9100 Herisau
Switzerland
Phone [phone]
Fax [phone]
[e-mail]
www.metrohm.com

IC equipment

IC equipment: Dialysis (6.5330.100)

8.110.8028EN / 2014-02-20

Manual

zst

Teachware
Metrohm AG
CH-9100 Herisau
[e-mail]

This documentation is protected by copyright. All rights reserved.

Although all the information given in this documentation has been
checked with great care, errors cannot be entirely excluded. Should you
notice any mistakes please send us your comments using the address
given above.

Documentation in additional languages can be found on

http://documents.metrohm.com.

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Table of contents

1 Introduction 1

1.1 Description of the IC equipment: Dialysis .......................... 1

1.2 About the documentation ................................................... 1

1.3 Symbols and conventions .................................................... 2

2 Overview 3

2.1 Parts of the IC equipment: Dialysis ..................................... 3

2.2 Parts of the dialysis cell ....................................................... 4

2.3 Dialysis cell connectors ........................................................ 4

2.4 Mode of operation for dialysis ............................................ 5

3 Installation 6

Table of contents

3.1 Preparing the dialysis cell .................................................... 6

3.2 Connecting the dialysis cell ............................................... 10

3.3 Inserting the dialysis cell .................................................... 14

3.4 Conditioning the dialysis system ....................................... 15

4 Operation and maintenance 16

4.1 Operation ............................................................................ 16

4.1.1 Optimizing the dialysis ........................................................... 16

4.1.2 Recommended procedure for the dialysis ............................... 18

4.2 Maintenance ....................................................................... 20

5 Technical specifications 21

5.1 Dialysis cell (6.2729.100) ................................................... 21

5.2 Dialysis membrane (6.2714.010) ....................................... 21

5.3 Dialysis membrane (6.2714.030) ....................................... 21

6 Accessories 22

Index 24

IC equipment: Dialysis

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Table of figures

Table of figures

Figure 1 IC equipment: Dialysis – Parts ............................................................ 3

Figure 2 Dialysis cell – Parts ............................................................................. 4

Figure 3 Dialysis cell – Connectors ................................................................... 4

Figure 4 Stopped-flow dialysis ......................................................................... 5

Figure 5 Area / transfer time diagram ............................................................ 17

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IC equipment: Dialysis

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1 Introduction

1.1 Description of the IC equipment: Dialysis

The IC equipment: Dialysis (6.5330.100) contains all accessories required
for inline dialysis of matrix-contaminated samples (e.g. emulsions, samples
containing fat and protein, body fluids or waste waters with high pollu­tion loads) directly before injection.

The main component of the IC equipment: Dialysis is the high-perfor­mance dialysis cell. The ions travel through the semipermeable membrane,
diffusing out of the flowing sample and into the stationary acceptor solu­tion, where they are preconcentrated. The ion-preconcentrated acceptor
solution is then subsequently injected directly into the IC system.

To operate the dialysis cell, you need an IC system with a peristaltic pump
and a cell holder as well as a Sample Processor with a peristaltic pump.

1 Introduction

1.2 About the documentation

This manual describes the correct assembly and maintenance of the IC
equipment: Dialysis as well as the installation of the capillary connections
to and from the dialysis cell. In addition, it describes how the dialysis
works and provides information on applying and optimizing the dialysis.

The installation of the peristaltic pump is not described in this manual. This
description can be found in the respective manuals for the ion chromato­graph or the Sample Processor.

CAUTION

Please read through this documentation carefully before putting the IC
equipment: Dialysis into operation. The documentation contains infor­mation and warnings which the user must follow in order to ensure
safe operation of the IC equipment: Dialysis.

IC equipment: Dialysis

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1.3 Symbols and conventions

1.3 Symbols and conventions

The following symbols and formatting may appear in this documentation:

Cross-reference to figure legend

The first number refers to the figure number, the sec­ond to the instrument part in the figure.

Instruction step

Carry out these steps in the sequence shown.

Method Dialog text, parameter in the software

File ▶ New Menu or menu item

[Next] Button or key

WARNING

This symbol draws attention to a possible life-threat­ening hazard or risk of injury.

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WARNING

This symbol draws attention to a possible hazard due
to electrical current.

WARNING

This symbol draws attention to a possible hazard due
to heat or hot instrument parts.

WARNING

This symbol draws attention to a possible biological
hazard.

CAUTION

This symbol draws attention to possible damage to
instruments or instrument parts.

NOTE

This symbol highlights additional information and
tips.

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IC equipment: Dialysis

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2 Overview

2.1 Parts of the IC equipment: Dialysis

2 Overview

IC equipment: Dialysis

Figure 1 IC equipment: Dialysis – Parts

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