Metrohm 761SD User Manual
761 SD Compact IC
CH-9101 Herisau/Schweiz
E-Mail info@metrohm.com
Internet www.metrohm.com
8.761.1043
Instructions for Use
CH-9101 Herisau/Schweiz
E-Mail info@metrohm.com
Internet www.metrohm.com
761 SD Compact IC
761 SD Compact IC
POWER
8.761.1043 Instructions for Use
8.761.1043 02.2004 / chs
Teachware
Metrohm AG
Oberdorfstrasse 68
CH-9101 Herisau
teachware@metrohm.com
1. Edition 2004
These instructions are protected by copyright. All rights reserved.
Although all the information given in these instructions has been checked with great care, errors
cannot be entirely excluded. Should you notice any mistakes please inform the author at the
address given above.
Table of contents
Table of contents
1 Introduction ................................................................ 1
1.1 Instrument description ................................................................................1
1.2 Parts and controls .......................................................................................3
1.2.1 Front view ........................................................................................................... 3
1.2.2 Rear view ............................................................................................................ 4
1.2.3 Connection schematic ....................................................................................... 5
1.3 Information on the Instructions for Use .....................................................7
1.3.1 Organisation ....................................................................................................... 7
1.3.2 Notation and pictograms.................................................................................... 8
1.4 Safety notes .................................................................................................9
1.4.1 Electrical safety................................................................................................... 9
1.4.2 General precautionary rules ............................................................................... 9
2 Installation ............................................................... 10
2.1 Flow chart ..................................................................................................10
2.2 Setting up the instrument..........................................................................11
2.2.1 Packaging.........................................................................................................11
2.2.2 Check................................................................................................................ 11
2.2.3 Location ............................................................................................................ 11
2.3 Description of the connections.................................................................12
2.3.1 Connection of capillaries/tubing ...................................................................... 12
2.3.2 Connection between capillaries/tubing............................................................ 13
2.3.3 Filter unit PEEK ................................................................................................. 14
2.4 Connection of the detector block .............................................................15
2.5 Installation of the MPak cabinet and connection of the drain tubes ......16
2.5.1 Installing the MPak cabinet .............................................................................. 16
2.5.2 Drain tube for inner compartment.................................................................... 16
2.5.3 Drain tube for MPak cabinet............................................................................. 16
2.6 Installing the eluent path ..........................................................................17
2.6.1 High-pressure pump – Removing the transport security screws .................. 17
2.6.2 Connection MPak → high-pressure pump ...................................................... 17
2.6.3 Connection high-pressure pump → injection valve ........................................ 18
2.6.4 Connection injection valve → suppressor .......................................................19
2.6.5 Connection suppressor → detector................................................................. 19
2.6.6 Connection detector → suppressor................................................................. 19
2.6.7 Connection suppressor → waste..................................................................... 20
2.7 Installing the regenerant path...................................................................20
2.7.1 Fitting the pump tubing for regenerant ............................................................ 20
2.7.2 Connection regenerant-MPak → pump tubing → suppressor → waste ........ 21
2.8 Installing the sample path.........................................................................23
2.8.1 Fitting the pump tubing for sample.................................................................. 23
2.8.2 Connection sample vessel → pump tubing → injection valve → waste ........ 24
2.9 Connecting the 766 IC Sample Processor ...............................................26
2.9.1 Installing the 766 IC Sample Processor ........................................................... 26
2.9.2 Connecting the 766 IC Sample Processor....................................................... 26
2.10 Fitting the rear panel .................................................................................27
2.11 Mains connections ....................................................................................28
2.11.1 Setting the mains voltage ................................................................................. 28
2.11.2 Fuses ................................................................................................................29
2.11.3 Mains cable and mains connection .................................................................29
2.11.4 Switching the instruments on/off ...................................................................... 29
761 SD Compact IC / Instructions for Use 8.761.1043
I
Table of contents
2.12 Connection to the PC ................................................................................30
2.12.1 Connecting cable 6.2134.100 .......................................................................... 30
2.12.2 Software installation ......................................................................................... 30
2.12.3 Basic settings «IC Net»..................................................................................... 31
2.12.4 Basic settings «IC Cap» ................................................................................... 33
2.13 Deaerating the pump and rinsing the pulsation dampener ....................34
2.13.1 Deaerating the pump ....................................................................................... 34
2.13.2 Rinsing the pulsation dampener ...................................................................... 35
2.14 Rinsing before fitting the column .............................................................37
2.15 Precolumn and separating column ..........................................................39
2.15.1 Metrosep RP Guard ......................................................................................... 39
2.15.2 Metrosep A Supp 1 HS separating column ..................................................... 40
2.16 Attaching tubing to side panels ................................................................42
3 «IC Net» ..................................................................... 43
3.1 «IC Net» – User interface for the 761 SD Compact IC..............................43
3.1.1 Systems - Methods .......................................................................................... 43
3.1.2 Opening a system ............................................................................................ 43
3.1.3 Opening a method ........................................................................................... 44
3.1.4 Connect a system ............................................................................................ 44
3.1.5 Instrument icon................................................................................................. 45
3.1.6 System parameters for linked system ............................................................. 45
3.1.7 Hardware settings ............................................................................................ 50
3.2 Systems supplied ......................................................................................54
3.2.1 System "startup.smt" ........................................................................................ 54
3.2.2 System "manual.smt"........................................................................................ 55
3.2.3 System "auto.smt" ............................................................................................ 56
3.2.4 System "shutdown.smt".................................................................................... 61
4 «IC Cap» ....................................................................64
4.1 «IC Cap» introduction ................................................................................64
4.1.1 Login................................................................................................................. 64
4.1.2 User interface ................................................................................................... 64
4.2 «IC Cap» - Configuration ...........................................................................66
4.2.1 Predefined configurations ................................................................................ 66
4.2.2 Configuration "manual.cfg"............................................................................... 66
4.2.3 Configuration "auto.cfg" ................................................................................... 72
5 Operation .................................................................. 77
5.1 Operation with manual sample change....................................................77
5.1.1 Control with «IC Net» (manual operation) ........................................................ 77
5.1.2 Control with «IC Cap» (manual operation) ....................................................... 81
5.2 Operation with automated sample change ..............................................84
5.2.1 Control with «IC Net» (automated operation)................................................... 84
5.2.2 Control with «IC Cap» (automated operation) ................................................. 86
6 Notes – Maintenance – Faults .................................. 88
6.1 Practical notes on ion chromatography ...................................................88
6.1.1 Separating columns ......................................................................................... 88
6.1.2 High-pressure pump ........................................................................................ 88
6.1.3 Eluents.............................................................................................................. 89
6.1.4 Peristaltic pump................................................................................................ 89
6.1.5 Suppressor module.......................................................................................... 89
6.1.6 Connections ..................................................................................................... 90
761 SD Compact IC / Instructions for Use 8.761.1043
II
Table of contents
6.2 Maintenance and servicing .......................................................................91
6.2.1 General information .......................................................................................... 91
6.2.2 Passivation........................................................................................................ 91
6.2.3 Shutdown.......................................................................................................... 91
6.2.4 Changing separating columns......................................................................... 92
6.2.5 Maintenance work on the pump head ............................................................. 92
6.2.6 Regeneration of the suppressor module .........................................................98
6.2.7 Cleaning the suppressor ................................................................................ 100
6.2.8 Replacing the suppressor .............................................................................. 102
6.2.9 Replacing the pump tubing............................................................................ 103
6.3 Faults and malfunctions..........................................................................104
6.3.1 Error messages .............................................................................................. 104
6.3.2 Malfunctions and their rectification ................................................................ 104
6.4 Diagnostic tests / Validation / GLP.........................................................106
7 Appendix ................................................................. 107
7.1 Technical data .........................................................................................107
7.1.1 Conductivity measurement ............................................................................ 107
7.1.2 Conductivity detector ..................................................................................... 107
7.1.3 Injection valve ................................................................................................. 108
7.1.4 High-pressure pump ...................................................................................... 108
7.1.5 Peristaltic pump.............................................................................................. 109
7.1.6 Suppressor module........................................................................................ 109
7.1.7 Leak detector.................................................................................................. 109
7.1.8 RS232 interface .............................................................................................. 109
7.1.9 Remote interface ............................................................................................ 110
7.1.10 Mains connection ...........................................................................................110
7.1.11 Safety specifications....................................................................................... 111
7.1.12 Electromagnetic compatibility (EMC)............................................................. 111
7.1.13 Ambient temperature...................................................................................... 111
7.1.14 Housing .......................................................................................................... 111
7.2 Standard equipment................................................................................112
7.3 Optional accessories ..............................................................................118
7.3.1 6.5328.000 SD Spare Part Set ....................................................................... 118
7.3.2 Other optional accessories ............................................................................120
7.4 Warranty and conformity.........................................................................121
7.4.1 Warranty.......................................................................................................... 121
7.4.2 Declaration of Conformity............................................................................... 122
7.4.3 Quality Management Principles .....................................................................123
7.5 Index.........................................................................................................124
761 SD Compact IC / Instructions for Use 8.761.1043
III
Table of contents
List of figures
Figure 1: Front of the 761 SD Compact IC ............................................................... 3
Figure 2: Rear panel 761 SD Compact IC ................................................................ 4
Figure 3: Connection schematic for 761 SD Compact IC ........................................ 5
Figure 4: Connectors for capillaries........................................................................ 13
Figure 5: PEEK couplings....................................................................................... 13
Figure 6: Connecting the filter unit PEEK ............................................................... 14
Figure 7: Position of the detector block 40 ............................................................ 15
Figure 8: Pump tubing for regenerant path ............................................................ 21
Figure 9: Flow schematic regenerant flow.............................................................. 22
Figure 10: Pump tubing for sample path.................................................................. 24
Figure 11: Flow schematic sample stream............................................................... 25
Figure 12: Rear panel ............................................................................................... 27
Figure 13: Setting the mains voltage........................................................................ 29
Figure 14: Connection of precolumn and separating column.................................. 41
Figure 15: Components of the pump head.............................................................. 94
Figure 16: Replacement of the piston seal 76.......................................................... 94
Figure 17: Components of inlet valve 77 and outlet valve 78.................................. 97
Figure 18: Assembling the suppressor .................................................................. 101
761 SD Compact IC / Instructions for Use 8.761.1043
IV
1.1 Instrument description
1 Introduction
1.1 Instrument description
The 761 SD Compact IC is a version of the time-proven 761 Compact
IC which has been developed specifically for soft drink analysis. The
761 SD Compact IC is designed for determining phosphoric acid and
its intermediate products in soft drinks but also continues to offer all
functionalities of the original version.
The advantages of the 761 SD Compact IC over the conventional
methods of phosphoric acid analysis in soft drinks are its short analysis
times, its ease of operation and the option for automated sampling.
There is no need for time-consuming sample preparation and only
small quantities of chemicals are consumed (more environmentally
friendly).
The 761 SD Compact IC has a very compact housing accommodating
all equipment required for ion-chromatographic determination:
• Injection valve – this supplies the sample to the eluent stream by
switching over the flow paths.
• High-pressure pump – extremely low-pulsation dual piston pump
with a flow range of 0.2 to 2.5 mL/min and a maximum pressure
of 25 MPa (250 bar).
• Pulsation dampener – the pulsation dampener protects the sepa-
rating column reliably against damage even in the case of low-level
pressure fluctuations.
• Column chamber – perfect insulation of the housing not only cre-
ates thermally stable conditions for the separating column, but also
shields the system against electromagnetic interference.
• Column – Metrosep A Supp 1 HS anion column as IC separating
column; Metrosep RP Guard as protective precolumn.
• Suppressor – the Metrohm-Suppressor-Module (MSM) which is
already integrated features stable pressure characteristics, automatic regeneration, high performance and optimum reproducibility.
• Peristaltic pump – integrated, two-channel peristaltic pump with a
flow rate of 0.5 to 0.6 mL/min for regeneration of the suppressor
module and aspiration of the sample for manual operation.
• Detector – conductivity detector with excellent temperature stabil-
ity. The detector temperature fluctuates by less than 0.01°C and
can be matched optimally to ambient conditions.
761 SD Compact IC / Instructions for Use 8.761.1043
1
1 Introduction
All components coming into contact with eluent and sample are metalfree. Ready-to-use eluents in certified MPaks are supplied for soft drink
analysis.
The 761 SD Compact IC is operated by means of a PC connected to
the RS232 interface. The 761 SD Compact IC can be controlled by the
«IC Net» software or by the “master” «IC Cap» software.
The systems required for soft drink analysis (and the methods linked to
them) are supplied on the installation CD.
The following instrument versions are available:
• 2.761.0420 761 SD Compact IC
• 2.761.0520 761 SD Compact IC with IC Sample Processor 766
for automated sample change
Principle:
The eluent flows on the eluent path through the injection valve into the
separating column and then into the detector. The sample flows on the
sample path also through the injection valve and the red sample loop
visible at the centre of the injection valve. At a specific instant (<Inject>; controlled by the program), the flow paths in the injection valve
are switched and the sample loop is reintegrated in the eluent path (it is
now no longer a part of the sample path). The sample quantity which is
located in the sample loop at the switchover instant is then entrained
with the eluent stream, thus entering the separating column (where the
actual chromatographic separation of the components occurs) and, finally, chromatographically separated, into the conductivity detector.
2
761 SD Compact IC / Instructions for Use 8.761.1043
1.2 Parts and controls
1.2 Parts and controls
In this section you will find the numbers and designations of the parts
and controls of the 761 SD Compact IC. The numbering applies
throughout the instructions for use, i.e. bold numbers in the text (e.g.
4
) refer to the parts and controls illustrated here.
1.2.1 Front view
761 SD Compact IC
6
7
POWER
Figure 1: Front of the 761 SD Compact IC
1
2
3
4
5
Door to inner compartment
1
Feedthrough for aspirating tubing
2
Aspirating tubing
3
for sample
Feedthrough for capillaries
4
761 SD Compact IC / Instructions for Use 8.761.1043
Connection for drain tube
5
for discharging escaped fluid from the
inner compartment
Connection purge valve
6
Pilot lamp
7
This is on when the instrument is
switched on
3
1 Introduction
1.2.2 Rear view
8
10
11
17
18
19
Type 1.761.
WARNING - Fire Hazard -
For continued protection replace only
with the same type and rating of fuse
Analog Output
Remote
Detector Block
RS 232
Waste B
Made by Metrohm Herisau Switzerland
9
Waste A
11
12
Transport securit y screws
13
14
100-120V:
220-240V:
Fuse
1,0A(T)
0,5A(T)
15
16
Figure 2: Rear panel 761 SD Compact IC
Opening for outlet capillaries
8
for discharge of eluent, regeneration
solution and sample solution
Opening for inlet capillaries
9
for supply of eluent, regeneration solution and sample solution
Opening for detector cable
10
Knurled screw
11
for fastening the rear panel
Detachable rear panel
12
Access to the inner compartment
Transport security screws
13
to secure the pump head when the instrument is transported
Mains switch
14
to switch instrument on and off:
I = ON 0 = OFF
20
21
Mains connection plug
15
Mains connection, see section 2.11
Fuse holder
16
Changing the fuses, see section 2.11.2
Serial number
17
Analogue output
18
output for analogue signal
Remote interface
19
Remote-I/O lines for connection of external devices
Connection for detector block
20
RS232 interface
21
PC connection
4
761 SD Compact IC / Instructions for Use 8.761.1043
1.2 Parts and controls
1.2.3 Connection schematic
23
24
55
27
28
26
56
29
30
25
31
32 33
34
35 37
36
43
54
38
56
39
40
41
42
48
45
47
44
50
63
64
3
50
61
65
48
56
46
55
49
22
Figure 3: Connection schematic for 761 SD Compact IC
3 Aspirating tubing
for sample
MPak cabinet
22
for suspending eluent and regenerant bag
Tubing connection to MPak
23
6.1837.000
Pump head 6.2824.100
24
Connection capillary
25
Connection between pump head and
purge valve, permanently mounted
Purge valve
26
23
Connection capillary
27
PEEK capillary 6.1831.010 for
deaerating,
length L = 15 cm
Connection capillary
28
PEEK capillary 6.1831.010,
length L = 13 cm
Connection capillary
29
PEEK capillary 6.1831.010,
length L = 13 cm
Pulsation dampener 6.2620.150
30
Inlet capillary for injector
31
PEEK capillary 6.1831.010,
length L = 24 cm
761 SD Compact IC / Instructions for Use 8.761.1043
5
1 Introduction
Sample loop 1.5 µL (6.1825.240)
32
PEEK sample loop
Column connection capillary
33
PEEK capillary 6.1831.010,
length L = 30 cm
Metrosep RP Guard 6.1011.020
34
Precolumn for protecting the separating column
Connection capillary
35
PEEK connection capillary between
precolumn and separating column
Metrosep A Supp 1 HS separating
36
column (6.1005.350)
IC separating column
Suppressor inlet capillary for elu-
37
ent ("Eluent")
PTFE capillary, permanently mounted
on suppressor, labelled "Eluent"
Suppressor outlet capillary for
38
eluent ("Detector")
PTFE capillary permanently mounted
on suppressor, labelled "Detector"
Inlet capillary to detector block
39
PEEK capillary, permanently
mounted
Detector block 1.732.0420
40
Outlet capillary from detector
41
block
PEEK capillary, permanently
mounted
Suppressor inlet capillary for elu-
42
ent ("H2O")
PTFE capillary, permanently mounted
on suppressor, labelled "H2O"
Suppressor module
43
Suppressor outlet capillary for
44
eluent ("Waste")
PTFE capillary, permanently mounted
on suppressor, leading to the waste,
labelled "Waste"
On instrument version 2.761.0520 with automated sample change
with the 766 IC Sample Processor, the aspirating tubing
by the PEEK capillary tubing
ple Processor) installed on the 766 IC Sample Processor. See Section
2.9. for installation of the 766 IC Sample Processor.
Suppressor inlet capillary for re-
45
generant
PTFE capillary, permanently mounted
on suppressor, labelled "H2SO4"
Suppressor outlet capillary for
46
regenerant ("Waste")
PTFE capillary, permanently mounted
on suppressor, leading into the
waste, labelled "Waste"
Connection capillary
47
PTFE capillary 6.1803.030, connection between pump tubing and injection valve
Connection capillary
48
PTFE capillary 6.1803.030, connection between injection valve and
waste container
Pump drive of the peristaltic
49
pump
Drive of the two-channel-peristaltic
pump for pumping sample and regenerant
50 Pressure screw 6.2744.010
54 PEEK coupling 6.2744.040
55 PEEK coupling 4.455.4500
Coupling for "tubing connection to
MPak"
56 Filter unit PEEK 6.2821.120
61 Pump tubing 6.1826.110 for H2SO
63 PEEK coupling 6.2744.030
64 Pump tubing 6.1826.110 for sam-
ple
65 PEEK coupling 6.2744.160
with tubing security device
3
is replaced
18
(see Instructions for Use 766 IC Sam-
4
6
761 SD Compact IC / Instructions for Use 8.761.1043
1.3 Information on the Instructions for Use
1.3 Information on the Instructions for Use
Please read through these Instructions for Use carefully before you put
the 761 SD IC Compact IC into operation. The Instructions for Use
contain information and warnings to which the user must pay attention
in order to assure safe operation of the instrument.
1.3.1 Organisation
These Instructions for Use 8.761.1043 for the 761 SD Compact IC
provide a comprehensive overview of installation, startup procedure,
operation, fault rectification and technical specifications of this instrument. The Instructions for Use are organised as follows:
Section 1 Introduction
General description of instrument, parts and controls and safety notes
Section 2 Installation
Installation and connection of the instrument, of the accessories and of the software
Section 3 «IC Net»
Explanatory information on the user interface of the «IC
Net» control software
Section 4 «IC Cap
Explanatory information on the user interface
of the «IC Cap» control software
Section 5 Operation
Description of operation with manual and
automatic sample change, with «IC Net» and
«IC Cap» in each case
Section 6 Notes – Maintenance – Faults
Notes on ion chromatography, maintenance, fault
rectification, diagnostic tests and validation
Section 7 Appendix
Technical data, standard equipment, options, warranty, declarations of conformity and index
To find the required information on the instruments, you will find it an
advantage to use either the Table of contents or the Index at the
back.
761 SD Compact IC / Instructions for Use 8.761.1043
7
1 Introduction
1.3.2 Notation and pictograms
The following notations and pictograms (symbols) are used in these Instructions for Use:
Fill Menu item, parameter or entry
value
in the software
SYSTEM STATE Program window
in the software
<OK> Button
in the software
20 Part or control of 761 SD
18 Part or control of 766
Hazard
This symbol draws attention to a
possible danger to life or of injury if
the associated directions are not
followed correctly. .
Warning
This symbol draws attention to
possible damage to instruments or
instrument parts if the associated
directions are not followed correctly.
Caution
This symbol marks important information. First read the associated
directions before you continue.
Comment
This symbol marks additional information and tips.
8
761 SD Compact IC / Instructions for Use 8.761.1043
1.4 Safety notes
1.4 Safety notes
1.4.1 Electrical safety
While electrical safety in the handling of the 761 SD Compact IC is assured in the context of the specifications IEC/EN 61010-1 (prot. class 1,
degree of protection IP20), the following points should be noted:
• Mains connection
Setting of the mains voltage, checking the mains fuse and the
mains connection must be effected in accordance with the instruc-
tions in section 2.11.
• Opening the 761 SD Compact IC
If the 761 SD Compact IC is connected to the power supply, the instrument must not be opened nor must parts be removed from it, otherwise there is a danger of coming into contact with components
which are live. Hence, always disconnect the instrument from all voltage sources before you open it and ensure that the mains cable is
15
disconnected from mains connection
!
• Protection against static charges
Electronic components are sensitive to static charging and can be
destroyed by discharges. Before you touch any of the components
inside the 761 SD Compact IC, you should earth yourself and any
tools you are using by touching an earthed object (e.g. housing of the
instrument or a radiator) to eliminate any static charges which exist.
1.4.2 General precautionary rules
• Handling of solvents
Check all lines of the IC system periodically for possible leaks. Follow
the relevant instructions regarding the handling of flammable and/or
toxic solvents and their disposal.
• Periodic exchange of pump tubing
Pump tubing is consumable material and must be replaced from time
to time (see Section 6.2.9). Please take suitable measures to ensure
that any leakage on pump tubing or connections in unattended continuous operation does not cause damage (position the instrument at
the bottom and provide a receptacle for catching escaping fluid).
761 SD Compact IC / Instructions for Use 8.761.1043
9
2 Installation
2 Installation
2.1 Flow chart
The following flow chart provides an overview of all installation work.
You will find more detailed information in the relevant section.
Setting up sect. 2.2
Installing detektor block sect. 2.4
Installing drain tubes
Installing eluent flow path sect. 2.6
Installing regenerant flow path sect. 2.7
Installing sample flow path
766 IC Sample
Processor
No
Replace rear panel
Mains connection
sect. 2.5
sect. 2.8
Yes
sect. 2.10
sect. 2.11
Connection to 766 IC Sample Processor
sect
. 2.9
Connecting PC sect
Installing software
Deaerating/Rinsing sect
Install guard and column sect. 2.15
10
761 SD Compact IC / Instructions for Use 8.761.1043
. 2.12
sect. 2.12.2
. 2.13 / 2.14
2.2 Setting up the instrument
2.2 Setting up the instrument
2.2.1 Packaging
The 761 SD Compact IC is supplied together with the separately
packed accessories in special packagings containing shock-absorbing
foam linings designed to provide excellent protection. The instrument
itself is packed in an evacuated polyethylene bag to prevent the ingress
of dust. Please store all these special packagings as only they assure
transport of the instrument free from damage.
2.2.2 Check
After receipt, immediately check whether the shipment is complete and
has arrived without damage (compare with delivery note and list of
accessories in section 7.2). In the case of transport damage, see
instructions in section 7.4.1 "Warranty".
2.2.3 Location
Position the instrument in the laboratory at a location convenient for operation, free from vibrations and protected against a corrosive atmosphere and contamination by chemicals.
To avoid disturbing temperature influences on the insulated column
compartment, the instrument must be protected against direct
sunlight.
761 SD Compact IC / Instructions for Use 8.761.1043
11
2 Installation
2.3 Description of the connections
2.3.1 Connection of capillaries/tubing
The connections for eluent, sample and regenerant consist of:
• PEEK capillaries 6.1831.010 (inner diameter = 0.25 mm)
• PTFE microcapillaries 6.1803.030 (inner diameter = 0.5 mm)
• PTFE tubing connections to the MPaks 6.1837.000 (inner di-
ameter = 1.5 mm)
The PEEK capillaries and PTFE microcapillaries can be connected either with PEEK pressure screws 50 6.2744.010 (long) or PEEK
pressure screws 51 6.2744.070 (short, for connection to the IC
pump).
The "PTFE tubing connection to the MPak" 23 can be connected to the
PEEK pressure screw 52 4.422.4510 (wide).
The connectors must be fitted to the capillaries as follows in this case:
Capillaries provided with new connectors must feature a flawless, flat
cut edge. In order to ensure this, it is best to use the tubing cutter
6.2621.080.
1 Fit pressure screw
Slide the corresponding pressure screw (50, 51 or 52), as
shown in Figure 4 onto the capillary 53 (23 for MPak connection).
2 Insert the capillary into the connection
Slide the end of the capillary fully into the corresponding
connector (in order to avoid dead volume).
3 Tighten the pressure screw
Firmly tighten the pressure screw (50, 51 or 52) by hand (do not
use tools).
12
761 SD Compact IC / Instructions for Use 8.761.1043
2.3 Description of the connections
50
23 Tubing connection to MPak
6.1837.000
Pressure screw 6.2744.010
50
53
51
Pressure screw 6.2744.070
51
for IC pump
Pressure screw 4.422.4510
52
23
52
for tubing connection 23 to MPak
Capillary
53
PEEK capillary 6.1831.010 or PTFE-
Figure 4: Connectors for ca-
microcapillary 6.1803.030
pillaries
2.3.2 Connection between capillaries/tubing
Capillaries and tubing can be connected together using PEEK couplings. If PEEK capillaries 6.1831.010 and/or PTFE microcapillaries
6.1803.030 are to be connected together, use the PEEK coupling
6.2744.040. If the "tubing connection to the MPak" is to be connected,
use the PEEK coupling 4.422.4500.
50
53
51
52
23
Figure 5: PEEK couplings
23 Tubing connection to MPak
6.1837.000
50 Pressure screw 6.2744.010
51 Pressure screw 6.2744.070
for IC pump
52 Pressure screw 4.422.4510
for tubing connection 23 to MPak
54
55
53 Capillary
PEEK capillary 6.1831.010 or PTFEmicrocapillary 6.1803.030
PEEK coupling 6.2744.040
54
PEEK coupling 4.455.4500
55
coupling for "tubing connection to the
MPak"
50
53
51
761 SD Compact IC / Instructions for Use 8.761.1043
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2 Installation
Capillaries may also be connected together via PEEK inline filters, see
Section 2.3.3.
2.3.3 Filter unit PEEK
Three PEEK filter units 6.2821.120 should be fitted in the 761 SD
Compact IC.
The first one should be fitted between IC pump head 24 and pulsation
dampener 30. It serves to avoid contamination by abrasive particles of
the piston seals.
The two other filter units are installed upstream of the suppressor module. One upstream of the inlet capillary 42 (labelled "H2O") in the return
path from the detector to the suppressor in the eluent path (see Section
2.6.6). The other one upstream of the inlet capillary 45 (labelled
"H2SO4"), between peristaltic pump and suppressor module in the regenerant path (see Section 2.7.2). They serve to protect the suppressor
module against foreign particles and bacterial growth.
For the connection of the filter unit, please note the flow direction arrow printed on the housing.
53
50
Figure 6: Connecting the filter unit PEEK
50 Pressure screw 6.2744.010
53 Capillary
PEEK capillary 6.1831.010 or PTFEmicrocapillary 6.1803.030
Filter unit PEEK 6.2821.120
56
56
50
53
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761 SD Compact IC / Instructions for Use 8.761.1043
2.4 Connection of the detector block
w
2.4 Connection of the detector block
The metal-free detector block 1.732.0420 which must be fitted in the
instrument and connected belongs to the scope of delivery of the 761
SD Compact IC. Proceed as follows:
1 Note the cell constant
• The cell constant c = XX.X /cm, measured at the works, is
printed on the rear side of the detector block. Note this value;
it must subsequently be entered in the software in order to
ensure that an exact display of the conductivity is obtained
(see Section 2.12.3).
2 Install detector block
• Unscrew the two upper knurled screws 11 from the upper
rear panel 12 of the 761 SD Compact IC, slacken the t
lower knurled screws 11 a little and remove the rear panel
(see Figure 2).
• Position the detector block 40 from the rear onto the space
provided in the 761 SD Compact IC and push it fully to the
front (see Figure 7).
o
39
41
3 Connect detector block
• Pull the grey connecting cable permanently attached to the
detector block 40 out through the opening (where the upper
rear panel 12 previously was) on the rear of the 761 SD
Compact IC and plug it in to connection 20 "Detector Block"
of the 761 SD Compact IC.
Since other connections need to be routed through the rear openings
of the 761 SD Compact IC later on, you should not fit the rear panel
back on until the end of the installation procedure (see Section 2.10).
40
39 Inlet capillary to detector block
PEEK capillary, permanently attached
40 Detector block 1.732.0420
41 Outlet capillary from detector block
PEEK capillary, permanently attached
12
40
Figure 7: Position of the detector block
761 SD Compact IC / Instructions for Use 8.761.1043
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2 Installation
39
The inlet and outlet capillaries (
block must be fitted in the eluent path as described in Sections 2.6.5
and 2.6.6.
and 41) to and from the detector
2.5 Installation of the MPak cabinet and connection of the drain tubes
2.5.1 Installing the MPak cabinet
Install the MPak cabinet as shown on the drawing accompanying the
package. Position it next to the 761 SD Compact IC and suspend the
eluent and regenerant MPaks from the crossbars. Since eluent and regenerant are supplied from the rear to the 761 SD Compact IC, the outlets of the MPaks should point to the rear.
2.5.2 Drain tube for inner compartment
The 761 SD Compact IC features a connector (5) for escaped fluids on
the front panel. A drain tube can be fitted to the connector. Proceed as
follows:
1 Connect drain tube
• Plug the silicone tube 6.1816.020 onto the nipple.
2 Route the drain tube into an outlet
• Route the other end of the drain tube into an outlet and se-
cure it at this point.
2.5.3 Drain tube for MPak cabinet
The basin of the MPak cabinet 22 features a connection for fluids escaping from the MPaks to which a drain tube can be fitted. Proceed as
follows:
1 Connect drain tube
• Plug the silicone tubing 6.1816.020 onto the nipple.
2 Route the drain tube into an outlet
• Route the other end of the drain tube into an outlet and se-
cure it there.
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761 SD Compact IC / Instructions for Use 8.761.1043
2.6 Installing the eluent path
2.6 Installing the eluent path
2.6.1 High-pressure pump – Removing the transport security screws
The pump head is locked with three transport security screws 13 in order to prevent damage to the pump drive during transport (see Figure
2). These transport security screws must be removed before placing
the high-pressure pump into operation. Also remove the red sticker attached to the pump head 24.
The three transport security screws must be refitted every time before
a major transport operation of the pump in order to avoid damage to
the pump head.
2.6.2 Connection MPak → high-pressure pump
The eluent MPak must be connected to the pump head 24 via the tubing connection 23 6.1837.000.
First connect the end at the pump head and then, not before, connect
the end at the MPak output. The eluent will start to drain out if you
connect the end at the MPak output first.
Procedure when connecting the tubing connection:
1 Connect the tubing connection using the PEEK
coupling to the pump head
• Connect the tubing connection 23 6.1837.000 using the
PEEK coupling 55 (already pre-fitted to the tubing) to the
capillary inlet of the pump head 24.
23
2 Connect the tubing connection to the eluent MPak
outlet
• Connect the other end of the tubing connection 23 to the elu-
52
ent MPak outlet (pierce the connection and turn it through 90°
clockwise).
55
51 53
761 SD Compact IC / Instructions for Use 8.761.1043
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2 Installation
2.6.3 Connection high-pressure pump → injection valve
In order to protect the column material against hammering effects owing to injection, the supplied pulsation dampener 6.2620.150 must
be fitted between high-pressure pump and injection valve of the 761 SD
Compact IC.
Connection between pump head and injection valve (see Figure 3):
Pump head 24 → connection capillary 25 → purge valve 26 → connection capillary 28 → inline filter 56 → connection capillary 29 →
pulsation dampener 30 → connection capillary 31 → injection valve
When delivered, a PEEK coupling 54 is fitted in place of the pulsation
dampener 30 between connection capillary 29 and 31. The PEEK coupling should be removed and the pulsation dampener should be fitted
at the same position. Proceed as follows:
1 Position the pulsation dampener
Place the pulsation dampener 30 6.2620.150 in the inner compartment of the 761 SD Compact IC on the bottom.
2 Remove the PEEK coupling
Remove the PEEK coupling 54 located between connection
capillary 29 and 31.
3 Connect the pulsation dampener
Connect the connection capillary 29 and connection capillary 31
to the pulsation dampener.
29
31
56
50
50
30
The pulsation dampener is filled with isopropanol and must be rinsed
with eluent before connection of a separating column (see Section
2.13.2).
The pulsation dampener 6.2620.150 can be operated in both directions.
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761 SD Compact IC / Instructions for Use 8.761.1043
2.6 Installing the eluent path
2.6.4 Connection injection valve → suppressor
The precolumn and the separating column are fitted between injection
valve and suppressor in the operational system (see Figure 3). How-
ever, since the system should first be rinsed without columns (so as not
to damage them), the columns may be fitted only later (see Section
2.15). A PEEK coupling 54 is fitted as a temporary replacement.
Proceed as follows:
1 Fit the PEEK coupling 54
Connect the column connection capillary 33 to the "suppressor
inlet capillary for eluent ("Eluent")" 37 via the PEEK coupling 54
(see Section 2.3.2).
Temporary connection schematic (c.f. Figure 3):
33
2.6.5 Connection suppressor → detector
From the suppressor, the eluent stream is routed on to the detector
(see Figure 3). The connection is made using a PEEK coupling. Proceed as follows:
1 Fit the PEEK coupling 54
Connect the "suppressor outlet capillary for eluent ("Detector")"
38 via the PEEK coupling 54 (see Section 2.3.2) to the "inlet ca-
pillary to detector block" 39.
37
54
2.6.6 Connection detector → suppressor
Downstream of the detector, the eluent flow is routed back into the
suppressor. It rinses the suppressor there after its regeneration phase.
A filter unit PEEK is fitted between detector and suppressor. Proceed as
follows:
1 Fit the filter unit PEEK
Connect the "outlet capillary from detector block" 41 via the filter
unit PEEK 54 (see Section 2.3.3) to the "suppressor inlet capillary for eluent ("H2O")" 42.
761 SD Compact IC / Instructions for Use 8.761.1043
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2 Installation
2.6.7 Connection suppressor → waste
Route the "suppressor outlet capillary for eluent ("Waste")" 44 at the rear
out of the 761 SD Compact IC into a waste container.
2.7 Installing the regenerant path
The regenerant (100 mmol/L H2SO4) is aspirated with the peristaltic
pump from the regenerant MPak and forced into the suppressor via the
filter unit PEEK. From the suppressor, it is then routed into a waste container (see Figure 9).
2.7.1 Fitting the pump tubing for regenerant
One channel of the two-channel peristaltic pump is used to pump the
regenerant. Fit a pump tubing 61 6.1826.110 as follows:
1 Disengage the tubing cartridges
• Detach and disengage the two tubing cartridges 57 above
the pump drive 49 by pressing in the snap-action lever 59 on
the retaining bracket.
• Press the pressure lever 58 down fully.
2 Fit the couplings 60
• Fit one coupling 60 6.2744.110 onto each end of the pump
tubing 61 6.1826.110. Moistening the tip of the coupling
lightly will make it easier to slip it on.
3 Fit the pump tubing
• Insert the fitted pump tubing 61 as shown in Figure 8 into the
tubing cartridge. The left-hand stopper 62 should latch into
the corresponding fixture on the left-hand side of the tubing
cartridge.
4 Reengage the tubing cartridge
• Reengage the tubing cartridge with pump tubing 61 in the re-
taining bracket. Engage it at the left first and then press the
right-hand side down until the snap-action lever 59 engages.
Ensure that the pump tubing is not kinked when doing this.
• Leave the other tubing cartridge outside. It is fitted with the
pump tubing for the sample path (see Section 2.8.1).
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761 SD Compact IC / Instructions for Use 8.761.1043
2.7 Installing the regenerant path
Tubing cartridge
57
Pressure lever
58
Snap-action lever
59
Coupling 6.2744.110
60
57
58
60
61
62
62
59
Figure 8: Pump tubing for regenerant path
Pump tubing 6.1826.110 for H
61
Stopper orange-yellow
62
60
2SO4
2.7.2 Connection regenerant-MPak → pump tubing → suppressor
→ waste
The connections to the pump tubing, suppressor and waste must be
connected first. Then, and not before, connect the end at the MPak
outlet. If the end at the MPak outlet is connected first, the regenerant
(H
Proceed as follows:
1 Connection Pump tubing - Suppressor
2 Connection Suppressor - Waste
) will begin to escape.
2SO4
• Screw the filter unit PEEK 56 6.2821.120 onto the coupling 60
6.2744.110 at the right-hand end (flow outlet) of the pump
tubing 61 fitted in the peristaltic pump. Note the flow direction
arrow on the filter unit.
• Firmly screw the "suppressor inlet capillary for regenerant
("H2SO4")" 45 onto the filter unit 56 with a pressure screw 50
6.2744.010 (see also Section 2.3.3).
• Route the "suppressor outlet capillary for regenerant
("Waste")" 46 at the rear out of the 761 SD Compact IC into a
waste container.
761 SD Compact IC / Instructions for Use 8.761.1043
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2 Installation
3 Connection MPak pump tubing
The regenerant MPak and pump tubing are connected using the
"tubing connection to MPak" 23 6.1837.000:
• First connect the end which is to be connected to the pump
tubing!
• Pressure screw 52 4.422.4510 and coupling 55 4.455.4500
are already pre-fitted on the "tubing connection to MPak" 23
6.1837.000. Screw this coupling 55 4.455.4500 onto the coupling 60 6.2744.110 plugged onto the left-hand end of the
pump tubing (flow inlet).
• Latch the other end of the "tubing connection to MPak" 23
6.1837.000 at the outlet of the regenerant MPak.
52
23
60
55
Figure 9: Flow schematic regenerant flow
23 Tubing connection to MPak
6.1837.000
45 Suppressor inlet capillary for re-
generant
PTFE capillary, permanently attached
to the suppressor, labelled "H2SO4"
46 Suppressor outlet capillary for re-
generant ("Waste")
PTFE capillary, permanently attached
to the suppressor; leads in to the
waste, labelled "Waste"
60
50
46
61
56
45
52 Pressure screw 4.422.4510
for "tubing connection to MPak" 23
55 PEEK coupling 4.455.4500
Coupling for "tubing connection to
MPak" 23
56 Filter unit PEEK 6.2821.120
60 Coupling 6.2744.110
61 Pump tubing 6.1826.110 for H2SO4
50 Pressure screw 6.2744.010
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761 SD Compact IC / Instructions for Use 8.761.1043
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