Evolve 3012
Integra Evolve 3012, Evolve 3016, Evolve 3013, Evolve 3015, Evolve 3018 Operating Instructions Manual
...
EVOLVE Manual Pipettes Operating instructions
900-00010-00_V00
Declaration of conformity
INTEGRA Biosciences Corp. –
Hudson, NH, USA
declares on its own responsibility that the devices
comply with:
Hudson, New Hampshire, USA - March 15, 2016
Description Models
EVOLVE Manual
Pipettes
3011, 3012, 3013, 3015, 3016, 3018, 3019, 3022, 3024, 3025,
3026, 3032, 3034, 3035, 3036
EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW
Restriction of Hazardous Substances 2011/65/EU
EU Regulations
Registration, Evaluation, Authorisation and Restriction of Chemicals
(REACH)
1907/2006
Piston-operated volumetric apparatus - Part 2: Piston pipettes ISO 8655-2
George Kalmakis
VP Operations
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Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................5
1.2 Intended use.....................................................................................5
1.3 Safety notes .....................................................................................6
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................7
2.2 Overview of the EVOLVE Manual Pipettes ......................................7
Chapter 3 Installation
3.1 Optional color coded plunger cap.....................................................8
3.2 Changing the Plunger Spring ...........................................................8
Chapter 4 Operation
4.1 Setting the volume..........................................................................10
4.2 Attaching and removing GripTip pipette tips .................................. 10
4.3 Pipetting .........................................................................................11
4.3.1 Aspirating and Dispensing ..................................................11
4.3.2 Reverse mode.....................................................................12
4.3.3 Proper pipetting techniques ................................................13
4.4 Troubleshooting..............................................................................14
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Chapter 5 Maintenance
5.1 Cleaning .........................................................................................15
5.2 Single channel lower end disassembly ..........................................15
5.2.1 Disassembly........................................................................15
5.2.2 Reassembly ........................................................................18
5.3 Sterilization.....................................................................................20
5.3.1 Autoclaving the disassembled components........................20
5.4 Servicing.........................................................................................21
5.4.1 Shipping to INTEGRA Biosciences.....................................21
5.4.2 Changing O-rings of tip fittings............................................21
5.4.3 Lubrication ..........................................................................22
5.5 Calibration ......................................................................................23
5.5.1 Materials .............................................................................23
5.5.2 Definitions ...........................................................................23
5.5.3 Test conditions and environment ........................................24
5.5.4 Pre-wetting of tips ...............................................................24
5.5.5 Leak test .............................................................................25
5.5.6 Obtaining the actual volume ...............................................26
5.5.7 Calculation of accuracy and precision ................................27
5.5.8 Adjusting EVOLVE Manual Pipettes...................................28
5.6 Equipment disposal ........................................................................29
Chapter 6 Technical Data
6.1 Environmental conditions ...............................................................30
6.2 Pipette specifications......................................................................30
6.3 Z Correction Factors.......................................................................32
Chapter 7 Accessories and Consumables
7.1 Accessories ....................................................................................33
7.2 Consumables .................................................................................33
Imprint................................................................................................... 36
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1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the EVOLVE Manual Pipettes. This chapter informs about the
symbols used in these operating instructions, the intended use of the EVOLVE Manual
Pipettes and the general safety instructions.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
W
ARNING
This safety symbol warns against hazards that could result in injury. It also
indicates hazards for machinery, materials and the environment.
It is essential that you follow the corresponding precautions.
C
AUTION
This symbol cautions against potential material damage. Follow the
instructions.
N
OTE
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
1.2 Intended use
EVOLVE Manual Pipettes are intended to be used in a laboratory for aspirating and
dispensing liquids in the volume range of 0.2–5000 µl using GripTip pipette tips.
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1.3 Safety notes
EVOLVE Manual Pipettes comply to the recognized safety regulations and is safe to
operate. EVOLVE Manual Pipettes can only be operated when in perfect condition and
while observing these operating instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the EVOLVE Manual Pipettes must have read
and understood these operating instructions, and particularly, the safety notes, or must
have been instructed by supervisors so that safe operation of the device is guaranteed.
Repairs may only be performed by INTEGRA Biosciences AG or by an authorized aftersales service member.
Parts may be replaced with original INTEGRA Biosciences parts only.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com
on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.
W
ARNING
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
CAUTION
Do not immerse the EVOLVE Manual Pipettes in liquid. The fluid can damage
internal parts. Avoid pipetting of liquids whose vapors could attack the
materials PA (polyamide), POM (polyoxymethylene), FPM (fluor-rubber),
NBR (nitrile-rubber), CR (chloroprene), silicone. Corrosive vapors could also
damage metallic parts inside the device.
N
OTE
Prolonged exposure of the EVOLVE Manual Pipettes to UV-light can cause
discoloration and/or yellowing of the pipette housing. However, this will not
affect the performance of the device in any way.
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2 Description of the device
2.1 Scope of delivery
• EVOLVE Manual Pipette
• Multipurpose Tool
• Replacement Plunger Cap
• Quick Start Guide
• Replacement O-rings (for 200 μl, 1000 μl and 5000 μl models)
2.2 Overview of the EVOLVE Manual Pipettes
1 Plunger Cap
2 Plunger, to pipette
3 Tip Ejector
4 Finger Hook
5 Adjustment Dials, to set volume
6 Volume Window
The color of the volume indicator label matches
GripTip box insert
7 Ejector Sleeve
8 Tip Fitting
1
2
4
6
3
5
7
8
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3 Installation
3.1 Optional color coded plunger cap
If desired, replace black plunger cap with optional color coded plunger cap.
3.2 Changing the Plunger Spring
The plunger spring installed inside the pipette is the light spring option. You can exchange
with a heavier plunger spring to achieve more control of the plunger movement.
1) Unscrew the Cylinder Assembly (a) and
separate the Piston Assembly (b, models 2–
200 µl) or Piston (model 1000 µl) from the upper
part of the pipette (c). It is held in place by a small
magnet.
N
OTE
5000 µl piston and cylinder assembly is
removed as one piece.
2) Set the pipette to the maximum setting. The
maximum setting is higher than the maximum
volume.
3) Put the two teeth of the Multipurpose Tool (d)
vertically into the slots of the black opening inside
at the bottom of the pipette‘s body (c). Rotate
counterclockwise until the plunger spring
assembly (e) is released.
a
b
c
d
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CAUTION
Validate or calibrate your pipette after reassembly.
4) To install the optional spring assembly, screw the
plunger spring assembly (e) clockwise into the
upper part of the pipette using the Multipurpose
Tool.
5) Re-attach the Piston assembly/Piston. Screw
clockwise to attach the Cylinder Assembly (a) to
the body of the pipette.
e
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4 Operation
4.1 Setting the volume
4.2 Attaching and removing GripTip pipette tips
CAUTION
T o ensure optimal performance of your EVOL VE Manual Pipettes always use
GripTips of correct size which have been designated for the use with the
EVOLVE Manual Pipettes, see “7.2
Consumables” on page 33.
The unique GripTip system of the EVOLVE Manual Pipettes reduces attachment and
ejection forces, ensures a perfect fitting to prevent the tips from falling off and to provide
a perfect seal. On a multichannel pipette all tips sit on the same height.
Attach the tips:
When loading tip(s), press the pipette into the appropriate GripTip(s) until you hear and
feel a click indicating that a seal has been achieved. Once you feel the click, stop applying
pressure. When loading GripTip pipette tips using a multichannel pipette, slowly rock the
pipette from one side to the other side to ensure the proper seal is achieved.
Discard your used tips:
If liquid is in the tips, empty them by pressing down the Plunger (2
) to the second stop
(see section 4.3
). Tips are easily removed by pressing the Tip Ejector (3).
1) To set the desired pipetting volume press down the Plunger (a)
and rotate slightly clockwise to unlock the volume dials.
2) Spin the Adjustment Dials (b) until the desired volume is
shown in the Volume Window.
3) Slightly rotate and slowly release the Plunger (a) to lock the
volume.
a
b
A rim inside the GripTips snaps over the multi-lobes and ensures
firm attachment of the tips.
A shoulder provides a positive stop to prevent over tightening of the
tips. Without hammering, the tip is either on or off but nothing in
between.
O-ring provides forgiving and robust seal surface for the pipette tip.
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4.3 Pipetting
The Plunger can be pushed down to two different stops:
For detailed recommendations for pipetting see also “4.3.3
Proper pipetting techniques”
on page 13.
4.3.1 Aspirating and Dispensing
To aspirate and dispense liquid perform the following steps:
1) Top position: Varies depending on volume setting.
2) First stop: Fixed calibrated position for aspirating and
dispensing.
3) Second Stop: To blowout the residual liquid.
Aspirate
1) With GripTip(s) attached, push down the Plunger (2
) to the first
stop.
2) Place GripTips in liquid. Hold the pipette vertically or in an angle
of max. 20° throughout the entire pipetting process and immerse
the GripTip(s) just below the surface of the liquid (2–3 mm).
3) Slowly return Plunger to the top position. For higher volumes
(>200 μl), follow the liquid level by moving the pipette down.
4) Remove the GripTip(s) from liquid.
Dispense
5) To dispense the liquid, push the Plunger (2
) down to the first
stop.
6) If there is any residual liquid in tip, a blowout
should be
performed by pressing the Plunger to the second stop.
7) Remove the GripTips from vessel and release Plunger.
1
2
3
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