Eppendorf Multipette Xstream User Manual
nualRepeater ® (X)stream
E
Register your instrument!
www.eppendorf.com/myeppendorf
N)
manual
Multipette®/Repeater ® Xstream
Multipette®/Repeater ® stream
Operating manual
Copyright© 2014 Eppendorf AG, Hamburg. All rights reserved, including graphics and
images. No part of this publication may be reproduced without the prior permission of the
copyright owner.
Eppendorf
®
and the Eppendorf logo are registered trademarks of Eppendorf AG,
Hamburg, Germany.
Combitips®, Combitips advanced®, Combitips plus®, Biopur®, Multipette stream®,
Multipette Xstream
®
, PhysioCare Concept®, Repeater® are registered trademarks of
Eppendorf AG, Hamburg, Germany.
Repeater stream™ and Repeater Xstream™ are protected trademarks of Eppendorf AG,
Hamburg, Germany.
Registered trademarks and protected trademarks are not marked in all cases with
®
or ™
in this manual.
4986 900.115-05/092014
Table of contents
Multipette
®
/Repeater ® (X)stream
English (EN)
Table of contents
1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.3.1 Operating modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.3.2 Dispensing tasks and ranges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.4 Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.5 Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.6 Overview Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.6.1 Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.6.2 Volume ranges for Combitips advanced . . . . . . . . . . . . . . . . . . . . 14
2.7 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.1 Mains/power supply device assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.2 Inserting the Li-ion rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.3 Charge the rechargeable battery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.4 Assembling the Combitip advanced and Adapter advanced. . . . . . . . . . . . . 21
4.5 Inserting the Combitip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.6 Removing the Combitip. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.6.1 Finishing the dispensing operation and removing the Combitip . 23
4.6.2 Emptying and removing the Combitip. . . . . . . . . . . . . . . . . . . . . . 23
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.1 Principle of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.2 Prepare the Multipette stream/Repeater stream. . . . . . . . . . . . . . . . . . . . . . 25
5.3 Parameters of the Multipette stream/Repeater stream . . . . . . . . . . . . . . . . . 26
5.4 Dispensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.5 Automatic dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.6 Sequential dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.7 Aspirating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Table o f con tents
Multipette
4
®
/Repeater ® (X)stream
English (EN)
5.8 Pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.9 Titration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.10 Stopping or canceling pipetting operations . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.11 Adjusting the device settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
6 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
6.1 Error search. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
6.2 Pipette self-test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
6.3 Hardware reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
7 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.2 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
8 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
8.1 Errors with the Multipette (X)stream/Repeater (X)stream . . . . . . . . . . . . . . 42
9 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.1 Multipette stream/Repeater stream . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.3 Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
9.4 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
10.1 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
10.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
10.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
11 Patents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Operating instructions
Multipette
®
/Repeater ® (X)stream
English (EN)
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time.
Please also note the operating instructions for the accessories, if applicable.
The instructions for use for the Combitips advanced can be found on our webpage
www.eppendorf.com/cbt
.
This operating manual is part of the product. Please keep it in a place that is easily
accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. You will find the current version on
our webpage www.eppendorf.com/worldwide
.
If information in this manual refers to the Multipette stream/Repeater stream, it is also
valid for the Multipette Xstream/Repeater Xstream. If information is only valid for the
Multipette Xstream/Repeater Xstream, this is explicitly mentioned in the text.
This manual applies to devices with software version 03.06.00 and higher. For devices
with older software and hardware, use the operating manuals Multipette (X)stream/
Repeater (X)stream 4986 900.115-01.
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and
danger levels:
5
1.2.1 Danger symbols
Biohazard Explosion
Electric shock Toxic substances
Hazard point Material damage
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WAR NIN G Can lead to severe injuries or death.
CAUTION Can lead to light to moderate injuries.
ATTENTION May lead to material damage.
Operating instructions
1
Multipette
6
®
/Repeater ® (X)stream
English (EN)
1.3 Symbols used
Depiction Meaning
1.
Actions in the specified order
2.
Actions without a specified order
• List:
Text Display or software texts
1.4 Glossary
A
Adapter advanced
Connecting piece for the dispenser when using Combitips advanced 25 mL and 50 mL
B
Biopur
Eppendorf Biopur
meets the requirements for standard products, e.g. precision, accuracy, wetting behavior,
tightness. Eppendorf Biopur
absence of ATP, PCR inhibitors, human and bacterial DNA, pyrogen, DNase and RNase.
Consumables of the purity grade Biopur are controlled and certified by an external
laboratory.
Certificates are available for downloading from our internet page www.eppendorf.com
Step in the figures
Direction of movement
Additional information
®
is an Eppendorf AG purity grade for consumables. Eppendorf Biopur®
®
also meets the requirements with regard to sterility,
.
C
Combitip advanced
Consumables for all Eppendorf Multipettes and Repeaters. With the Combitip advanced,
the dispenser becomes an operational dispenser. Combitips advanced are intended for
single use. Combitips advanced consist of a piston and a cylinder and function according
to the positive displacement principle. They are offered for nine different volume ranges.
For Combitips advanced with a maximum volume of 25 mL or 50 mL, the Adapter
advanced is also required.
Operating instructions
Multipette
®
/Repeater ® (X)stream
English (EN)
F
Free jet dispensing
Dispensing the liquid without the Combitip touching the tube inner wall. If a drip forms
on the Combitip after free jet dispensing, this drip always belongs to the next dispensing
step. We recommend completing a dispensing series using only the free jet method or
wall dispensing method. Complete the reverse stroke in accordance with the dispensing
series using either the free jet or wall dispensing method. Errors listed in the technical
data were determined using the wall dispensing method.
I
Increment
Step size or resolution. For instance, when selecting the volume for a 10 mL Combitip, the
volume can be increased or reduced in 0.01 mL steps.
ISO 8655
The ISO 8655 standard defines limiting values for systematic errors [accuracy], random
errors [precision]) and the test methods for dispensing devices.
N
Nominal volume
The maximum dispensing volume of a Combitip in conjunction with the selected
dispensing device. The term "nominal volume" comes from the ISO 8655 standard.
7
P
PhysioCare Concept®
Products that carry the Eppendorf PhysioCare Concept logo feature optimized
ergonomics and make operation as simple as possible, even with complex dispensing
tasks. PhysioCare products can be easily serviced and cleaned. They are made of
extremely light materials and require minimal operating force.
Positive displacement principle
The liquid comes into direct contact with the Combitip piston during aspiration and
dispensing. Unlike with a pipette, the liquid and piston are not separated by an air
cushion. A little air bubble is visible at the piston during dispensing.
R
Rechargeable lithium-ion battery
Rechargeable lithium-ion batteries have a very high charging capacity and long service
life.
Reverse stroke
After aspiration, the piston is moved into a defined position. Liquid is dispensed during
this piston movement. The reverse stroke is not a dispensing step.
Operating instructions
Multipette
8
®
/Repeater ® (X)stream
English (EN)
V
Viscosity
Viscosity describes the viscidity of liquids and suspensions. The dynamic or absolute
viscosity is indicated in Pa·s or in mPa·s. In older literature, the unit P or cP is used
(1 mPa·s corresponds to 1 cP).
At room temperature, a 50 % glycerin solution has a viscosity of approx. 6 mPa·s. As the
glycerin concentration increases, viscosity increases considerably. Absolutely anhydrous
glycerin has a viscosity of approx. 1480 mPa · s at room temperature.
2 Product description
Asp
Seq
Ads
Ttr
Pip
Dis
+ / –
reset
select
1
2
3
4
5
6
7
8
9
10
2.1 Main illustration
Abb. 2-1:Front view
Product description
Multipette
®
/Repeater ® (X)stream
English (EN)
1 Connector socket
For connecting the charging plug of an Eppendorf
Multipette stream/Repeater stream power supply
device.
2 Hardware reset button
Triggering a hardware reset interrupts the power
supply (see p. 40).
3 Selection dial
For setting the operating mode.
Depending on the Multipette stream/Repeater
stream version, different operating modes are
available. The illustration shows the Xstream.
4 Display (illuminated)
Displays the parameter settings (volume, dispensing
steps, speeds) and provides feedback and
information on the operating steps.
5 Select rocker switch
For switching between the execution and editing
displays.
6Reset key
For stopping or canceling dispensing operations
(see p. 34).
7+/- rocker switch
For changing parameters. Higher speed level with
volume change when you press the +/- rocker switch
more firmly.
8Actuate key
For triggering dispensing operations. For saving
entries during editing and for returning to
dispensing.
9Ejector
Releases the inserted Combitip.
10 Combitip advanced
Dispenser tip
9
Fig. 2-1: Front view
Product description
Multipette
®
Xstream
1
2
3
4
Multipette
10
English (EN)
®
/Repeater ® (X)stream
Abb. 2-2:Side view
1 Viewing window for the infrared interface
For Service.
2 Battery compartment lid
For covering the battery compartment. The battery
compartment contains the serial number of the
Multipette stream/Repeater stream.
3 Charging contact and mounting
For the battery charging option and for fixing the
Multipette stream/Repeater stream in the charging
stand (additionally available).
4Finger rest
For ensuring a secure and comfortable grip.
Fig. 2-2: Side view
2.2 Delivery package
Quantity Description
1 Multipette stream
or Multipette Xstream
or Repeater stream
or Repeater Xstream
1 Combitips advanced 1.0 mL
1 Adapter advanced 25 mL
1 Adapter advanced 50 mL
1 Li-ion rechargeable battery
1 Power supply with power plug adapters
1 Operating Manual Multipette/Repeater (X)stream
1 Short instructions Multipette/Repeater (X)stream
Product description
Multipette
®
/Repeater ® (X)stream
English (EN)
2.3 Features
In combination with a Combitip, the Multipette stream/Repeater stream is a motor-driven
and electronically controlled manual dispenser which operates according to the positive
displacement principle.
2.3.1 Operating modes
The Multipette stream/Repeater stream and Multipette Xstream/Repeater Xstream have
the following operating modes:
Dis Ads Seq Asp Pip Ttr
Multipette stream/Repeater stream – – –
Multipette Xstream/Repeater Xstream
Dis Dispensing gradual dispensing of liquid in equal partial volumes.
Ads Automatic
dispensing
Seq Sequential
dispensing
automatic dispensing of liquid in equal partial volumes
and at fixed intervals.
dispensing liquid in up to 16 different volumes in one
sequence.
Asp Aspirating consecutive aspiration of liquid of the same volume
into a Combitip.
Pip Pipetting aspirating and dispensing liquid.
Ttr Titration gradual dispensing of reagent into a sample solution.
Multiple aspiration of the reagent into the Combitip is
possible.
11
2.3.2 Dispensing tasks and ranges
It is possible to dispense aqueous solutions, but also solutions with a higher viscosity. At a
higher viscosity, lower aspiration and dispensing speeds should be selected.
• If the lowest speed (1 bar) is used for aspiration and dispensing, it is possible to
dispense solutions with a viscosity of ≤ 300 mPa·s with ≤ 25 mL Combitips.
• If 50 mL Combitips are used, the viscosity must not exceed 100 mPa·s.
• With 0.5 mL Combitips and the highest speed setting, 5 L of water can be dispensed
in a free jet. Free jet dispensing of water is also possible with all larger Combitips. If
large volumes are dispensed in a free jet, the liquid may splash back!
For solutions with a higher vapor pressure it is recommended to saturate the
small air bubble at the piston of the Combitip with liquid vapor by aspirating and
dispensing the liquid several times.
Product description
Multipette
12
®
/Repeater ® (X)stream
English (EN)
2.4 Warranty
In the case of warranty claims, please contact your responsible Eppendorf contractual
partner. No warranty is provided for any damage due to misuse or if the device has been
opened by unauthorized persons. The rechargeable lithium-ion battery and all other wear
parts are excluded from the warranty.
2.5 Display
The display has 10 brightness levels. The display switches off during usage intervals. The
display switches on again automatically when a key is pressed. If the Multipette stream/
Repeater stream is not used for an extended period of time, the display is switched off
completely after approx. 10 minutes.
The Multipette stream/Repeater stream saves the most recent setting for each Combitip
size in all operating modes. If you are using a new Combitip in the corresponding size, the
most recent setting will be shown on the display.
Multipette
20
•
µL
100.0
2
3
4
1
6
5
Product description
®
/Repeater ® (X)stream
English (EN)
13
Abb. 2-3:Display
Fig. 2-3: Display
1Volume
Unit in µL or mL.
2 Direction of the next piston movement
Aspirate liquid
Dispense liquid
3Key lock
is only displayed if the +/- rocker switch is locked.
Can be stored or activated for the device parameters
(see p. 34).
4 Battery
Battery charging status. Possible displays:
Battery full
Battery exhausted
Battery severely discharged
Battery empty
If the battery is exhausted, complete the dispensing
operation and charge the battery. If the battery is
severely discharged, or Warning Battery Low!
appears on the display, stop the dispensing
operation immediately and charge the battery for at
least two hours.
5 Dispensing step
In the Dis, Ads and Seq modes, the number of
possible dispensing steps. In the Asp mode, the
number of performed Asp steps
6 Interpunct
Is displayed in the Dis, Ads and Asp modes before
aspiration. Indicator for the number of dispensing
steps. The pipetted volume must always be greater
than the product of the multiplication of the volume
(1) with the dispensing step (5).
2.6 Overview Combitips advanced
Combitips advanced are disposable devices for aspirating and dispensing liquids
according to the positive displacement principle. Combitips advanced are used in
Eppendorf dispensers (e.g., Multipettes/Repeaters) and are made up of a cylinder and a
piston. Combitips advanced in sizes 25 mL and 50 mL require an Adapter advanced.
Combitips advanced are available in various sizes, which are marked using a color code.
Product description
^
0.1 mL 0.2 mL 0.5 mL 1 mL 2.5 mL 5 mL 10 mL 25 mL 50 mL
Multipette
14
®
/Repeater ® (X)stream
English (EN)
2.6.1 Combitips advanced
2.6.2 Volume ranges for Combitips advanced
Combitips advanced Volume range Increment
0.1 mL
1.0 µL – 100 µL 0.1 µL
white
0.2 mL
2.0 µL – 200 µL 0.2 µL
light blue
0.5 mL
5.0 µL – 500 µL 0.5 µL
purple
1.0 mL
10 µL – 1000 µL 1.0 µL
yellow
2.5 mL
25 µL – 2500 µL 2.5 µL
green
5.0 mL
50 µL – 5000 µL 5.0 µL
blue
10 mL
0.1 mL – 10 mL 0.01 mL
orange
25 mL
red
50 mL
0.25 mL – 25 mL 0.025 mL
0.5 mL – 50 mL 0.05 mL
light gray
Product description
Multipette
®
/Repeater ® (X)stream
English (EN)
2.7 Materials
NOTICE! Aggressive substances may damage dispensers, Combitips and
accessories.
Check the chemical resistance when using organic solvents or aggressive
chemicals.
Please note the cleaning instructions.
The components of the Multipette stream/Repeater stream which can be accessed by the
user are made from the following materials:
Component Material
Housing parts Acrylonitrile/styrene/acrylic ester (ASA)
with polycarbonate (PC)
Select rocker switch, +/- rocker switch Silicone
Actuate key Polyetherimide (PEI)
Charging contacts Gold coating
Display, selection dial, window IR interface Polycarbonate (PC), coated and uncoated
Combitip advanced Material
Cylinder Polypropylene (PP)
Piston 0.1 mL and 0.2 mL Polyethylene (PE) with glass fiber (GF)
Piston 0.5 mL to 50 mL Polyethylene (PE)
Adapter advanced Polybutylene terephthalate (PBT)
15
Safety
Multipette
16
English (EN)
®
/Repeater ® (X)stream
3Safety
3.1 Intended use
The Multipette stream/Repeater stream is a lab device and in combination with a
Combitip advanced it is intended for dispensing aqueous solutions in the volume range of
1 µL – 50 mL. In vivo applications (applications in or on the human body) are not
permitted.
The !!! use DEV_MODEL !!!! may only be operated by trained specialists. All users must
have read the operating manual carefully and familiarized themselves with the device's
mode of operation.
The Multipette stream/Repeater stream is not suitable for the permanent
dispensing of fuming acids.
3.2 Warnings for intended use
DANGER! Risk of explosion.
Do not operate the device in areas where work is completed with explosive
substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any materials that could create an explosive
atmosphere.
WARNING! Damage to health from infectious liquids and pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.
Wear personal protective equipment.
Follow the instructions regarding hygiene, cleaning and decontamination.
Comprehensive regulations on handling germs or biological material in risk
group II or higher can be found in the "Laboratory Biosafety Manual" (source:
World Health Organization, Laboratory Biosafety Manual, in the currently
valid version).
WARNING! Damage to health due to toxic, radioactive or aggressive
chemicals.
Wear personal protective equipment.
Observe the national regulations for handling these substances.
Observe the material safety data sheets and manufacturer's application notes.
Multipette
CAUTION! Danger to persons from careless use
®
/Repeater ® (X)stream
Safety
English (EN)
Never point the opening of a Multipette stream/Repeater stream which is
equipped with a Combitip at yourself or other persons.
Only initiate dispensing if it is safe to do so.
With any dispensing task please ensure that you do not endanger yourself
and other persons.
Check the set speed before commencing dispensing operations. For the
dispensing operation select a speed which excludes the back-splashing of the
liquid.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by
Eppendorf may impair the safety, functioning and precision of the device.
Eppendorf cannot be held liable or accept any liability for damage resulting from
the use of incorrect or non-recommended accessories and spare parts, or from
the improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
NOTICE! Carry-over, contamination and incorrect dispensing results due to
the incorrect use of Combitips.
Combitips are intended for single use. Prolonged use can have a negative impact
on dispensing accuracy.
Only use Combitips once.
Do not use washed and/or autoclaved Combitips for dispensing.
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NOTICE! Damage to the device due to penetration of liquids.
Do not allow any liquids to penetrate the inside of the housing.
If liquid has entered the inside of the housing, the inner parts may only be
repaired by Eppendorf AG service partners. Contact your responsible sales
office before returning a product.
NOTICE! Terminating or interrupting dispensing due to incorrect handling.
If the Combitip is handled incorrectly, an error message may appear on the
display or a safety shutdown may be triggered.
Do not bend the Combitip.
During dispensing or aspirating, do not press the Combitip too strongly
against the bottom of the tube or against the tube wall.
Safety
Multipette
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English (EN)
®
/Repeater ® (X)stream
NOTICE! Material damage due to incorrect dispensing.
When using the larger Combitips and highly viscous solutions the Multipette
may heat up.
Only use highly viscous solutions for short dispensing series.
NOTICE! Incorrect dispensing results due to evaporation.
If dispensing is continued with a Combitip that has already been filled after a
waiting time, the dispensing volume in the next dispensing step may be slightly
reduced due to evaporation.
If a high degree of accuracy is required, omit this dispensing step.
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised.
Liability for any resulting property damage or personal injury is then transferred to the
operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
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