nualRepeater ® (X)stream
E
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N)
manual
Multipette®/Repeater ® Xstream
Multipette®/Repeater ® stream
Operating manual
Copyright© 2014 Eppendorf AG, Hamburg. All rights reserved, including graphics and
images. No part of this publication may be reproduced without the prior permission of the
copyright owner.
Eppendorf
®
and the Eppendorf logo are registered trademarks of Eppendorf AG,
Hamburg, Germany.
Combitips®, Combitips advanced®, Combitips plus®, Biopur®, Multipette stream®,
Multipette Xstream
®
, PhysioCare Concept®, Repeater® are registered trademarks of
Eppendorf AG, Hamburg, Germany.
Repeater stream™ and Repeater Xstream™ are protected trademarks of Eppendorf AG,
Hamburg, Germany.
Registered trademarks and protected trademarks are not marked in all cases with
®
or ™
in this manual.
4986 900.115-05/092014
Table of contents
Multipette
®
/Repeater ® (X)stream
English (EN)
Table of contents
1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.3.1 Operating modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.3.2 Dispensing tasks and ranges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.4 Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.5 Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.6 Overview Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.6.1 Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.6.2 Volume ranges for Combitips advanced . . . . . . . . . . . . . . . . . . . . 14
2.7 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.1 Mains/power supply device assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.2 Inserting the Li-ion rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.3 Charge the rechargeable battery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.4 Assembling the Combitip advanced and Adapter advanced. . . . . . . . . . . . . 21
4.5 Inserting the Combitip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.6 Removing the Combitip. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.6.1 Finishing the dispensing operation and removing the Combitip . 23
4.6.2 Emptying and removing the Combitip. . . . . . . . . . . . . . . . . . . . . . 23
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.1 Principle of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.2 Prepare the Multipette stream/Repeater stream. . . . . . . . . . . . . . . . . . . . . . 25
5.3 Parameters of the Multipette stream/Repeater stream . . . . . . . . . . . . . . . . . 26
5.4 Dispensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.5 Automatic dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.6 Sequential dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.7 Aspirating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Table o f con tents
Multipette
4
®
/Repeater ® (X)stream
English (EN)
5.8 Pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.9 Titration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.10 Stopping or canceling pipetting operations . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.11 Adjusting the device settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
6 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
6.1 Error search. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
6.2 Pipette self-test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
6.3 Hardware reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
7 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.2 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
8 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
8.1 Errors with the Multipette (X)stream/Repeater (X)stream . . . . . . . . . . . . . . 42
9 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.1 Multipette stream/Repeater stream . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.3 Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
9.4 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
10.1 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
10.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
10.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
11 Patents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Operating instructions
Multipette
®
/Repeater ® (X)stream
English (EN)
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time.
Please also note the operating instructions for the accessories, if applicable.
The instructions for use for the Combitips advanced can be found on our webpage
www.eppendorf.com/cbt
.
This operating manual is part of the product. Please keep it in a place that is easily
accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. You will find the current version on
our webpage www.eppendorf.com/worldwide
.
If information in this manual refers to the Multipette stream/Repeater stream, it is also
valid for the Multipette Xstream/Repeater Xstream. If information is only valid for the
Multipette Xstream/Repeater Xstream, this is explicitly mentioned in the text.
This manual applies to devices with software version 03.06.00 and higher. For devices
with older software and hardware, use the operating manuals Multipette (X)stream/
Repeater (X)stream 4986 900.115-01.
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and
danger levels:
5
1.2.1 Danger symbols
Biohazard Explosion
Electric shock Toxic substances
Hazard point Material damage
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WAR NIN G Can lead to severe injuries or death.
CAUTION Can lead to light to moderate injuries.
ATTENTION May lead to material damage.
Operating instructions
1
Multipette
6
®
/Repeater ® (X)stream
English (EN)
1.3 Symbols used
Depiction Meaning
1.
Actions in the specified order
2.
Actions without a specified order
• List:
Text Display or software texts
1.4 Glossary
A
Adapter advanced
Connecting piece for the dispenser when using Combitips advanced 25 mL and 50 mL
B
Biopur
Eppendorf Biopur
meets the requirements for standard products, e.g. precision, accuracy, wetting behavior,
tightness. Eppendorf Biopur
absence of ATP, PCR inhibitors, human and bacterial DNA, pyrogen, DNase and RNase.
Consumables of the purity grade Biopur are controlled and certified by an external
laboratory.
Certificates are available for downloading from our internet page www.eppendorf.com
Step in the figures
Direction of movement
Additional information
®
is an Eppendorf AG purity grade for consumables. Eppendorf Biopur®
®
also meets the requirements with regard to sterility,
.
C
Combitip advanced
Consumables for all Eppendorf Multipettes and Repeaters. With the Combitip advanced,
the dispenser becomes an operational dispenser. Combitips advanced are intended for
single use. Combitips advanced consist of a piston and a cylinder and function according
to the positive displacement principle. They are offered for nine different volume ranges.
For Combitips advanced with a maximum volume of 25 mL or 50 mL, the Adapter
advanced is also required.
Operating instructions
Multipette
®
/Repeater ® (X)stream
English (EN)
F
Free jet dispensing
Dispensing the liquid without the Combitip touching the tube inner wall. If a drip forms
on the Combitip after free jet dispensing, this drip always belongs to the next dispensing
step. We recommend completing a dispensing series using only the free jet method or
wall dispensing method. Complete the reverse stroke in accordance with the dispensing
series using either the free jet or wall dispensing method. Errors listed in the technical
data were determined using the wall dispensing method.
I
Increment
Step size or resolution. For instance, when selecting the volume for a 10 mL Combitip, the
volume can be increased or reduced in 0.01 mL steps.
ISO 8655
The ISO 8655 standard defines limiting values for systematic errors [accuracy], random
errors [precision]) and the test methods for dispensing devices.
N
Nominal volume
The maximum dispensing volume of a Combitip in conjunction with the selected
dispensing device. The term "nominal volume" comes from the ISO 8655 standard.
7
P
PhysioCare Concept®
Products that carry the Eppendorf PhysioCare Concept logo feature optimized
ergonomics and make operation as simple as possible, even with complex dispensing
tasks. PhysioCare products can be easily serviced and cleaned. They are made of
extremely light materials and require minimal operating force.
Positive displacement principle
The liquid comes into direct contact with the Combitip piston during aspiration and
dispensing. Unlike with a pipette, the liquid and piston are not separated by an air
cushion. A little air bubble is visible at the piston during dispensing.
R
Rechargeable lithium-ion battery
Rechargeable lithium-ion batteries have a very high charging capacity and long service
life.
Reverse stroke
After aspiration, the piston is moved into a defined position. Liquid is dispensed during
this piston movement. The reverse stroke is not a dispensing step.
Operating instructions
Multipette
8
®
/Repeater ® (X)stream
English (EN)
V
Viscosity
Viscosity describes the viscidity of liquids and suspensions. The dynamic or absolute
viscosity is indicated in Pa·s or in mPa·s. In older literature, the unit P or cP is used
(1 mPa·s corresponds to 1 cP).
At room temperature, a 50 % glycerin solution has a viscosity of approx. 6 mPa·s. As the
glycerin concentration increases, viscosity increases considerably. Absolutely anhydrous
glycerin has a viscosity of approx. 1480 mPa · s at room temperature.
2 Product description
Asp
Seq
Ads
Ttr
Pip
Dis
+ / –
reset
select
1
2
3
4
5
6
7
8
9
10
2.1 Main illustration
Abb. 2-1:Front view
Product description
Multipette
®
/Repeater ® (X)stream
English (EN)
1 Connector socket
For connecting the charging plug of an Eppendorf
Multipette stream/Repeater stream power supply
device.
2 Hardware reset button
Triggering a hardware reset interrupts the power
supply (see p. 40).
3 Selection dial
For setting the operating mode.
Depending on the Multipette stream/Repeater
stream version, different operating modes are
available. The illustration shows the Xstream.
4 Display (illuminated)
Displays the parameter settings (volume, dispensing
steps, speeds) and provides feedback and
information on the operating steps.
5 Select rocker switch
For switching between the execution and editing
displays.
6Reset key
For stopping or canceling dispensing operations
(see p. 34).
7+/- rocker switch
For changing parameters. Higher speed level with
volume change when you press the +/- rocker switch
more firmly.
8Actuate key
For triggering dispensing operations. For saving
entries during editing and for returning to
dispensing.
9Ejector
Releases the inserted Combitip.
10 Combitip advanced
Dispenser tip
9
Fig. 2-1: Front view
Product description
Multipette
®
Xstream
1
2
3
4
Multipette
10
English (EN)
®
/Repeater ® (X)stream
Abb. 2-2:Side view
1 Viewing window for the infrared interface
For Service.
2 Battery compartment lid
For covering the battery compartment. The battery
compartment contains the serial number of the
Multipette stream/Repeater stream.
3 Charging contact and mounting
For the battery charging option and for fixing the
Multipette stream/Repeater stream in the charging
stand (additionally available).
4Finger rest
For ensuring a secure and comfortable grip.
Fig. 2-2: Side view
2.2 Delivery package
Quantity Description
1 Multipette stream
or Multipette Xstream
or Repeater stream
or Repeater Xstream
1 Combitips advanced 1.0 mL
1 Adapter advanced 25 mL
1 Adapter advanced 50 mL
1 Li-ion rechargeable battery
1 Power supply with power plug adapters
1 Operating Manual Multipette/Repeater (X)stream
1 Short instructions Multipette/Repeater (X)stream
Product description
Multipette
®
/Repeater ® (X)stream
English (EN)
2.3 Features
In combination with a Combitip, the Multipette stream/Repeater stream is a motor-driven
and electronically controlled manual dispenser which operates according to the positive
displacement principle.
2.3.1 Operating modes
The Multipette stream/Repeater stream and Multipette Xstream/Repeater Xstream have
the following operating modes:
Dis Ads Seq Asp Pip Ttr
Multipette stream/Repeater stream – – –
Multipette Xstream/Repeater Xstream
Dis Dispensing gradual dispensing of liquid in equal partial volumes.
Ads Automatic
dispensing
Seq Sequential
dispensing
automatic dispensing of liquid in equal partial volumes
and at fixed intervals.
dispensing liquid in up to 16 different volumes in one
sequence.
Asp Aspirating consecutive aspiration of liquid of the same volume
into a Combitip.
Pip Pipetting aspirating and dispensing liquid.
Ttr Titration gradual dispensing of reagent into a sample solution.
Multiple aspiration of the reagent into the Combitip is
possible.
11
2.3.2 Dispensing tasks and ranges
It is possible to dispense aqueous solutions, but also solutions with a higher viscosity. At a
higher viscosity, lower aspiration and dispensing speeds should be selected.
• If the lowest speed (1 bar) is used for aspiration and dispensing, it is possible to
dispense solutions with a viscosity of ≤ 300 mPa·s with ≤ 25 mL Combitips.
• If 50 mL Combitips are used, the viscosity must not exceed 100 mPa·s.
• With 0.5 mL Combitips and the highest speed setting, 5 L of water can be dispensed
in a free jet. Free jet dispensing of water is also possible with all larger Combitips. If
large volumes are dispensed in a free jet, the liquid may splash back!
For solutions with a higher vapor pressure it is recommended to saturate the
small air bubble at the piston of the Combitip with liquid vapor by aspirating and
dispensing the liquid several times.
Product description
Multipette
12
®
/Repeater ® (X)stream
English (EN)
2.4 Warranty
In the case of warranty claims, please contact your responsible Eppendorf contractual
partner. No warranty is provided for any damage due to misuse or if the device has been
opened by unauthorized persons. The rechargeable lithium-ion battery and all other wear
parts are excluded from the warranty.
2.5 Display
The display has 10 brightness levels. The display switches off during usage intervals. The
display switches on again automatically when a key is pressed. If the Multipette stream/
Repeater stream is not used for an extended period of time, the display is switched off
completely after approx. 10 minutes.
The Multipette stream/Repeater stream saves the most recent setting for each Combitip
size in all operating modes. If you are using a new Combitip in the corresponding size, the
most recent setting will be shown on the display.
Multipette
20
•
µL
100.0
2
3
4
1
6
5
Product description
®
/Repeater ® (X)stream
English (EN)
13
Abb. 2-3:Display
Fig. 2-3: Display
1Volume
Unit in µL or mL.
2 Direction of the next piston movement
Aspirate liquid
Dispense liquid
3Key lock
is only displayed if the +/- rocker switch is locked.
Can be stored or activated for the device parameters
(see p. 34).
4 Battery
Battery charging status. Possible displays:
Battery full
Battery exhausted
Battery severely discharged
Battery empty
If the battery is exhausted, complete the dispensing
operation and charge the battery. If the battery is
severely discharged, or Warning Battery Low!
appears on the display, stop the dispensing
operation immediately and charge the battery for at
least two hours.
5 Dispensing step
In the Dis, Ads and Seq modes, the number of
possible dispensing steps. In the Asp mode, the
number of performed Asp steps
6 Interpunct
Is displayed in the Dis, Ads and Asp modes before
aspiration. Indicator for the number of dispensing
steps. The pipetted volume must always be greater
than the product of the multiplication of the volume
(1) with the dispensing step (5).
2.6 Overview Combitips advanced
Combitips advanced are disposable devices for aspirating and dispensing liquids
according to the positive displacement principle. Combitips advanced are used in
Eppendorf dispensers (e.g., Multipettes/Repeaters) and are made up of a cylinder and a
piston. Combitips advanced in sizes 25 mL and 50 mL require an Adapter advanced.
Combitips advanced are available in various sizes, which are marked using a color code.
Product description
^
0.1 mL 0.2 mL 0.5 mL 1 mL 2.5 mL 5 mL 10 mL 25 mL 50 mL
Multipette
14
®
/Repeater ® (X)stream
English (EN)
2.6.1 Combitips advanced
2.6.2 Volume ranges for Combitips advanced
Combitips advanced Volume range Increment
0.1 mL
1.0 µL – 100 µL 0.1 µL
white
0.2 mL
2.0 µL – 200 µL 0.2 µL
light blue
0.5 mL
5.0 µL – 500 µL 0.5 µL
purple
1.0 mL
10 µL – 1000 µL 1.0 µL
yellow
2.5 mL
25 µL – 2500 µL 2.5 µL
green
5.0 mL
50 µL – 5000 µL 5.0 µL
blue
10 mL
0.1 mL – 10 mL 0.01 mL
orange
25 mL
red
50 mL
0.25 mL – 25 mL 0.025 mL
0.5 mL – 50 mL 0.05 mL
light gray
Product description
Multipette
®
/Repeater ® (X)stream
English (EN)
2.7 Materials
NOTICE! Aggressive substances may damage dispensers, Combitips and
accessories.
Check the chemical resistance when using organic solvents or aggressive
chemicals.
Please note the cleaning instructions.
The components of the Multipette stream/Repeater stream which can be accessed by the
user are made from the following materials:
Component Material
Housing parts Acrylonitrile/styrene/acrylic ester (ASA)
with polycarbonate (PC)
Select rocker switch, +/- rocker switch Silicone
Actuate key Polyetherimide (PEI)
Charging contacts Gold coating
Display, selection dial, window IR interface Polycarbonate (PC), coated and uncoated
Combitip advanced Material
Cylinder Polypropylene (PP)
Piston 0.1 mL and 0.2 mL Polyethylene (PE) with glass fiber (GF)
Piston 0.5 mL to 50 mL Polyethylene (PE)
Adapter advanced Polybutylene terephthalate (PBT)
15
Safety
Multipette
16
English (EN)
®
/Repeater ® (X)stream
3Safety
3.1 Intended use
The Multipette stream/Repeater stream is a lab device and in combination with a
Combitip advanced it is intended for dispensing aqueous solutions in the volume range of
1 µL – 50 mL. In vivo applications (applications in or on the human body) are not
permitted.
The !!! use DEV_MODEL !!!! may only be operated by trained specialists. All users must
have read the operating manual carefully and familiarized themselves with the device's
mode of operation.
The Multipette stream/Repeater stream is not suitable for the permanent
dispensing of fuming acids.
3.2 Warnings for intended use
DANGER! Risk of explosion.
Do not operate the device in areas where work is completed with explosive
substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any materials that could create an explosive
atmosphere.
WARNING! Damage to health from infectious liquids and pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.
Wear personal protective equipment.
Follow the instructions regarding hygiene, cleaning and decontamination.
Comprehensive regulations on handling germs or biological material in risk
group II or higher can be found in the "Laboratory Biosafety Manual" (source:
World Health Organization, Laboratory Biosafety Manual, in the currently
valid version).
WARNING! Damage to health due to toxic, radioactive or aggressive
chemicals.
Wear personal protective equipment.
Observe the national regulations for handling these substances.
Observe the material safety data sheets and manufacturer's application notes.
Multipette
CAUTION! Danger to persons from careless use
®
/Repeater ® (X)stream
Safety
English (EN)
Never point the opening of a Multipette stream/Repeater stream which is
equipped with a Combitip at yourself or other persons.
Only initiate dispensing if it is safe to do so.
With any dispensing task please ensure that you do not endanger yourself
and other persons.
Check the set speed before commencing dispensing operations. For the
dispensing operation select a speed which excludes the back-splashing of the
liquid.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by
Eppendorf may impair the safety, functioning and precision of the device.
Eppendorf cannot be held liable or accept any liability for damage resulting from
the use of incorrect or non-recommended accessories and spare parts, or from
the improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
NOTICE! Carry-over, contamination and incorrect dispensing results due to
the incorrect use of Combitips.
Combitips are intended for single use. Prolonged use can have a negative impact
on dispensing accuracy.
Only use Combitips once.
Do not use washed and/or autoclaved Combitips for dispensing.
17
NOTICE! Damage to the device due to penetration of liquids.
Do not allow any liquids to penetrate the inside of the housing.
If liquid has entered the inside of the housing, the inner parts may only be
repaired by Eppendorf AG service partners. Contact your responsible sales
office before returning a product.
NOTICE! Terminating or interrupting dispensing due to incorrect handling.
If the Combitip is handled incorrectly, an error message may appear on the
display or a safety shutdown may be triggered.
Do not bend the Combitip.
During dispensing or aspirating, do not press the Combitip too strongly
against the bottom of the tube or against the tube wall.
Safety
Multipette
18
English (EN)
®
/Repeater ® (X)stream
NOTICE! Material damage due to incorrect dispensing.
When using the larger Combitips and highly viscous solutions the Multipette
may heat up.
Only use highly viscous solutions for short dispensing series.
NOTICE! Incorrect dispensing results due to evaporation.
If dispensing is continued with a Combitip that has already been filled after a
waiting time, the dispensing volume in the next dispensing step may be slightly
reduced due to evaporation.
If a high degree of accuracy is required, omit this dispensing step.
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised.
Liability for any resulting property damage or personal injury is then transferred to the
operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
Multipette
®
/Repeater ® (X)stream
Installation
English (EN)
4Installation
4.1 Mains/power supply device assembly
3
1 Power plug adapters
a EU
b United Kingdom
c USA
d Australia
2Mains/power supply device
3 Charging plug
Insert the power plug adapter required for your mains/power supply into the opening
of the mains/power supply device. If there is any doubt as to which power plug adapter
should be used for the power supply unit, you should consult an electrician.
19
4.2 Inserting the Li-ion rechargeable battery
Abb. 4-1:Insert the Li-ion rechargeable battery
1 Connector socket
2Plug
3 Li-ion rechargeable battery
1. Take off the lid of the rechargeable
battery compartment.
2. Insert the Li-ion battery. Do not remove
the foam insert in the rechargeable
battery compartment.
3. Connect the plug of the Li-ion
Fig. 4-1: Insert the Li-ion rechargeable
battery
rechargeable battery to the connector
socket.
4. Replace the rechargeable battery
compartment lid.
Insert the rechargeable battery as
follows:
Installation
Multipette
20
®
/Repeater ® (X)stream
English (EN)
4.3 Charge the rechargeable battery
WARNING! Incorrect or damaged mains/power supply devices can lead to
severe personal injury or damage to the device.
Incorrect or damaged mains/power supply devices may cause electric shocks,
cause the device to overheat, catch fire, melt, short circuit or similar damage.
Only use the mains/power supply device which is included in the delivery for
charging the battery. You can recognize the correct mains/power supply
device from the Eppendorf logo and the device name on the power supply.
Do not use damaged mains/power supply devices.
The supplied Multipette stream/Repeater stream can only be charged with the
new power supply unit (order number 4986 603.005) included in this delivery. It
is technically not possible to charge this Multipette stream/Repeater stream with
a power supply unit from an older delivery. However, older Multipette stream/
Repeater stream models can be charged with the new power supply unit
included in this delivery. Every charging stand of the Multipette stream/Repeater
stream can be operated with a new power supply unit.
To identify the power supply, each unit is provided with a label with the names
"Multipette stream/Xstream", "Repeater stream/Xstream" and "Xplorer". The
power supply is designed for the range from 100 - 240 V.
4986 603.005
Multipette
Repeater
Xplorer
®
stream/Xstream
®
stream/Xstream
®
Recharging the rechargeable battery
1. Insert the assembled power supply (order number 4986 603.005) into a power outlet.
2. Check in the Multipette stream/Repeater stream whether a rechargeable battery has
been inserted and connected.
3. Insert the charging plug of the power supply into the connector socket of the
Multipette stream/Repeater stream.
The message Device connected to power supply is briefly displayed. The charging
process is indicated in the display by a flashing rechargeable battery symbol. A fully
discharged accumulator requires a charging time of at least two hours. The charging
process is complete when the accumulator symbol stops flashing.
The rechargeable battery only achieves its full capacity after several charging cycles.
Multipette
You can also use the additionally available charger stand 4880 or the charger
carousel 4880 (see Accessories on p. 45).
To charge the Multipette stream/Repeater stream using the charger
carousel 4880, you must order a suitable charger shell for the Multipette stream/
Repeater stream in addition.
®
/Repeater ® (X)stream
Installation
English (EN)
4.4 Assembling the Combitip advanced and Adapter advanced
Combitips advanced with a maximum volume of 0.1 mL – 10 mL can be used immediately.
Combitips advanced with a maximum volume of 25 mL and 50 mL can only be used with
the corresponding Adapter advanced. Adapter advanced and Combitips advanced have
the same color code. The maximum volume is also listed on the neck of the Adapter
advanced.
NOTICE! Sensor damage due to damaged or worn adapters.
Always put the adapter and Combitip together outside of the dispenser.
Do not used damaged or worn adapters.
Do not use adapters with damaged coding.
1. Place the adapter on the Combitip.
2. Tighten the adapter.
21
Installation
12
1
2
Multipette
22
®
/Repeater ® (X)stream
English (EN)
4.5 Inserting the Combitip
NOTICE! Device damage due to incorrect handling of the inserted Combitip
Insert the Combitip straight into the dispenser from below.
Do not rotate the inserted Combitip.
Never grip the dispenser using the Combitip.
The Combitip is easier to insert if the ejector is held pressed down during
insertion.
1. If the Combitip piston has been pushed
out, push it back into the cylinder of the
Combitip.
2. Use force to push the Combitip into the
opening on the lower side of the
dispenser until the Combitip engages
.
Multipette
2.Short = empty
1.Short = no step
Long = aspirate
Release
Combitip now
Release
Combitip now
®
/Repeater ® (X)stream
Installation
English (EN)
4.6 Removing the Combitip
CAUTION! Personal injury and damage to the Multipette stream/Repeater
stream and to the Combitip by operating the ejector at the wrong time.
If you eject the Combitip during dispensing, liquid may escape. If the ejector is
operated at the wrong time, only the cylinder of the Combitip is released. The
piston can only be released if it is in the basic position.
Only operate the ejector when the piston in the Combitip has reached its
lowest position.
If you accidentally operate the ejector during dispensing, and the cylinder is released
because of this, an error message appears (see Error search on p. 36).
After dispensing you can remove the Combitip. If any liquid remains in the Combitip, it
needs to be emptied first.
4.6.1 Finishing the dispensing operation and removing the Combitip
1. Finish all dispensing steps.
The following information is displayed:
2. Hold the Multipette stream/Repeater stream over a tube.
3. Press the actuate key twice to move the piston to the basic position and to dispense the
liquid remaining in the Combitip in a safe manner.
The following information is briefly displayed:
23
4. Press the ejector button and remove the Combitip.
4.6.2 Emptying and removing the Combitip
If you would like to terminate dispensing prematurely, you need to empty the remaining
liquid out of the Combitip before you can remove it.
1. Hold the Multipette stream/Repeater stream over a tube.
2. Press the reset key to move the piston to the basic position and to dispense the liquid
remaining in the Combitip in a safe manner.
The following information is briefly displayed:
3. Press the ejector button and remove the Combitip.
24
1234
1 2 3 4
1
2
next step
Discard
Operation
Multipette
®
/Repeater ® (X)stream
English (EN)
5Operation
5.1 Principle of operation
This chapter is provided for information purposes only and is not intended to provide
instructions. All operating modes have a comparable procedure. The following steps
occur in all operating modes except the aspiration (Asp) operating mode:
Parameter settings
1. The parameters that are available can vary depending on the operating mode. For each
Combitip size the most recently set parameters are saved.
• Dispensing volume (Pip)
• Dispensing volume per partial dispensing step (Dis, Ads, Seq)
• Aspiration volume (Asp)
• Number of dispensing steps (Dis, Ads, Seq, Asp)
• Aspiration speed (all operating modes)
• Dispensing speed (all operating modes)
• Interval time (Ads)
Aspirating liquid
2. You can stop aspiration by pressing the actuate or reset key. When you stop aspiration,
the reduced number of dispensing steps is not stored for the next dispensing
operation.
Reverse stroke
3. Positioning the piston of the Combitip. Liquid escapes. This step is a safety step and
not a dispensing step. The reverse stroke is indicated in the display as follows:
Multipette
3
4
Changed data
stored
®
/Repeater ® (X)stream
Operation
English (EN)
Dispensing steps
The liquid dispensing angle should always be as steep as possible. A dispensing
angle greater than 45° can result in an incorrect dispensing volume during the
final dispensing steps.
4. Dispense liquid. You can change some parameters during dispensing.
5. There are two possible variants for continuing execution:
• Renewed aspiration
Perform further dispensing process with the previously set parameters.
• Empty the Combitip completely
Dispense the residual liquid from the Combitip to finish dispensing and to release
the Combitip.
5.2 Prepare the Multipette stream/Repeater stream
This chapter describes general operating instructions for Multipette stream/Repeater
stream. The Multipette stream/Repeater stream and Multipette Xstream/
Repeater Xstream differ as regards the available operating modes (see p. 11). Setting the
parameters is identical for every operating mode.
Proceed as follows to set the operating mode and the parameters:
At high viscosity, select low aspiration and dispensing speeds. Solutions with a
viscosity up to 300 mPa·s must be dispensed using a Combitip with a maximum
size of 25 mL, set at the lowest speed. If a 50 mL Combitip is used, the viscosity
must not exceed 100 mPa·s.
1. Select a suitable Combitip and insert.
The size of the Combitip is recognized automatically by the Multipette stream/Repeater
stream and shown on the display for approx. 1 second.
2. If the Multipette stream/Repeater stream is in sleep mode, press any key to activate it.
3. Set the desired operating mode using the selection dial. The selected mode is shown
temporarily in the display.
The start display shows the parameters of the last operation with a Combitip of the
same size.
4. Setting the parameters . It is also possible to change some parameters in all operating
modes during operation. For this, press the select rocker switch during operation and
set the parameters.
• Change between the parameters using the select rocker switch.
• Change the parameters using the +/- rocker switch.
5. Press the actuate key to save your settings.
The display shows:
25
Operation
50
Set volume
•
mL
0.20
4
Set volume:
μL
50
50
Set volume
•
mL
0.20
50
Set steps:
Speed
Multipette
26
English (EN)
®
/Repeater ® (X)stream
If you eject the Combitip before pressing the actuate key, the changes will not be
saved.
CAUTION! Personal injury and damage to the Multipette stream/Repeater
stream and to the Combitip by operating the ejector at the wrong time.
If you eject the Combitip during dispensing, liquid may escape. If the ejector is
operated at the wrong time, only the cylinder of the Combitip is released. The
piston can only be released if it is in the basic position.
Only operate the ejector when the piston in the Combitip has reached its
lowest position.
6. You can find further details in the chapter on your selected operating mode.
Dispensing is performed with the actuate key. If you press the actuate key or the reset
key during liquid aspiration or dispensing operations, you will interrupt the dispensing
operation.
5.3 Parameters of the Multipette stream/Repeater stream
Display Parameter function Dis Ads Seq Asp Pip Ttr
Dispensing volume
Depends on the size of the
inserted Combitip.
Dispensing volume per
dispensing step
Depends on the size of the
inserted Combitip. Up to
16 different volumes can be
selected.
Aspiration volume
Depends on the size of the
inserted Combitip.
Number of dispensing steps
The number of dispensing steps
depends on the dispensing
volumes.
Aspiration speed
10 speeds are available. The
total of number of bars filled in
black represents the speed level
selected. The higher the speed,
the lower the electric power
consumption.
– ––
–––––
–––––
– –
Multipette
Speed
1.0 s
Set time:
1234
1
2
next step
Discard
®
/Repeater ® (X)stream
Operation
English (EN)
Display Parameter function Dis Ads Seq Asp Pip Ttr
Dispensing speed
Select the highest speed for
dispensing small volumes in free
jet mode. Select a low speed for
viscous solutions.
If there is a risk of liquid
splashing out of the destination
tube during dispensing, reduce
the dispensing speed.
Interval time
Distance of time between the
dispensing steps. You can
–––––
choose times between 0.1 - 10 s.
5.4 Dispensing
During dispensing the aspirated liquid is
dispensed step by step in partial volumes of
equal size. With the smallest dispensing
volume and the Combitip completely filled,
100 dispensing steps are possible.
27
1. Prepare the Multipette stream/Repeater stream.
2. Immerse the Combitip into the liquid to be aspirated.
3. Press the actuate key. Liquid is aspirated.
The display will then show:
4. Hold the Multipette stream/Repeater stream over a waste container or the supply
vessel and press the actuate key. This step is not a dispensing step.
The reverse stroke is performed.
5. Hold the Combitip over the first tube into which the liquid is to be dispensed.
6. Press the actuate key to dispense the liquid. Repeat the process for every additional
dispensing step. After each dosing step, the display will show the volume of the next
dispensing step and the number of the remaining dispensing steps.
After complete dispensing, the display shows:
28
2.Short = empty
1.Short = no step
Long = aspirate
1234 6
5
1 2
next step
Discard
Operation
Multipette
®
/Repeater ® (X)stream
English (EN)
7. There are two possible variants for continuing execution:
• Renewed aspiration
a Immerse the Combitip into the liquid to be dispensed.
b Press and hold the actuate key for 2 seconds to aspirate the liquid.
• Empty the Combitip fully and remove
a Hold the Multipette stream/Repeater stream over a tube to dispense the residual
liquid.
b Press the actuate key twice to empty the residual liquid.
c Remove the Combitip.
5.5 Automatic dispensing
The Ads mode is carried out automatically if
you press and hold the actuate key. For
longer dispensing series (filling plates) in
particular, this mode is more convenient
and quicker than the Dis mode. The
dispensing volume, the dispensing speed
and the interval time can be changed during
dispensing.
1. Prepare the Multipette stream/Repeater stream (see p. 25). When filling plates with
96 wells we recommend you set the interval time between 0.6 s and 2 s, depending on
the liquid.
2. Immerse the Combitip into the liquid to be aspirated.
3. Press the actuate key.
Liquid is aspirated.
The display will then show:
4. Hold the Multipette stream/Repeater stream over a waste container or the supply
vessel and press the actuate key. The reverse stroke is performed. This step is not a
dispensing step.
5. Hold the Combitip over the first tube into which the liquid is to be dispensed.
6. Press and hold the actuate key to aspirate the liquid automatically. Move the Multipette
stream/Repeater stream to the next tube within the interval time set previously. If the
actuate key is released, the next dispensing steps will not be carried out. You can
continue dispensing afterwards by pressing and holding the actuate key.
Multipette
2.Short = empty
1.Short = no step
Long = aspirate
1234 6
5
1234 6
5
1 2
®
/Repeater ® (X)stream
After complete dispensing, the display shows:
7. There are two possible variants for continuing execution:
• Renewed aspiration
a Immerse the Combitip into the liquid to be dispensed.
b Press and hold the actuate key for 2 seconds to aspirate the liquid.
• Completely empty the Combitip and remove
a Hold the Multipette stream/Repeater stream over a tube to dispense the residual
liquid.
b Press the actuate key twice to empty the residual liquid.
c Remove the Combitip.
5.6 Sequential dispensing
(only Multipette Xstream/Repeater Xstream)
Operation
29
English (EN)
This mode is particularly suitable for dilution series. Sequential dispensing is carried out
with two different liquids with the same volumes in two directions. Different dilutions can
be prepared for the same total volume. In the Seq mode, up to 16 different volumes can
be dispensed. You can change the speed during dispensing. The dispensing speed applies
to all dispensing volumes. The number of dispensing steps and the different dispensing
volumes cannot be changed during execution. If the total of all dispensing volumes
exceeds the nominal volume of the Combitip, "nnnnn" is displayed for the subsequent
dispensing steps. We recommend to delete these dispensing steps before starting
dispensing to avoid any operating errors. To do this, reduce the number of dispensing
steps in the Set steps parameter.
1. Prepare the Multipette stream/Repeater stream.
2. Immerse the Combitip into the liquid to be aspirated.
30
next step
Discard
2.Short = empty
1.Short = no step
Long = aspirate
1234
21
Operation
Multipette
®
/Repeater ® (X)stream
English (EN)
3. Press the actuate key to aspirate the liquid.
The display will then show:
4. Hold the Multipette stream/Repeater stream over a waste container or the supply
vessel and press the actuate key.
The reverse stroke is performed. This step is not a dispensing step.
5. Hold the Combitip over the first tube into which the liquid is to be dispensed.
6. Press the actuate key to dispense the liquid. Repeat the process for each additional
tube.
The display shows the dispensing volume and the number of the next dispensing step.
After complete dispensing, the display shows:
7. There are two possible variants for continuing execution:
• Renewed aspiration
a Immerse the Combitip into the liquid to be dispensed.
b Press and hold the actuate key for 2 seconds to aspirate the liquid.
• Completely empty the Combitip and remove
a Hold the Multipette stream/Repeater stream over a tube to dispense the residual
liquid.
b Press the actuate key twice to empty the residual liquid.
c Remove the Combitip.
5.7 Aspirating
(only Multipette Xstream/Repeater Xstream)
1. Prepare the Multipette stream/Repeater stream. To prevent the whirling up of sediment
or to observe a phase limit, select a low aspiration speed.
In the Asp mode you aspirate a defined
volume several times consecutively into the
Combitip, for instance, to take a specific
volume from the wells of a plate. The Asp
mode is performed approximately in
reverse to the Dis mode. The aspiration
volume, aspiration step and speed can be
changed during aspiration. After aspiration
of the partial volumes you dispense the total
volume into a tube.
Multipette
Press key for
special step
next step
Discard
Release
Combitip now
1 2
next step
Discard
®
/Repeater ® (X)stream
Operation
English (EN)
2. Press the actuate key.
The display shows:
3. Hold the Multipette stream/Repeater stream over a waste container or the supply
vessel and press the actuate key to trigger a "special step" without aspiration of liquid.
The reverse stroke is performed. This step is not a dispensing step.
4. Immerse the Combitip into the liquid to be aspirated. Press the actuate key to aspirate
the liquid. Repeat the process for every additional well.
The display shows the aspiration volume and the number of the next aspiration step.
5. Press the actuate key again.
The display then shows:
6. Hold the Combitip over the tube into which the liquid is to be dispensed.
7. Press the actuate key to dispense the liquid.
After dispensing, the following is shown temporarily in the display:
8. Remove the Combitip.
5.8 Pipetting
31
1. Prepare the Multipette stream/Repeater stream.
2. Immerse the Combitip into the liquid to be aspirated.
3. Press the actuate key to aspirate the liquid.
The display shows:
If you only wish to dispense a volume once,
use of the Pip mode is recommended. The
Pip mode can also be used to mix a solution
by repeated aspirating and dispensing. If a
solution must be dispensed at low speed in
the Dis mode, but quickly mixed before,
this can be accomplished by combining
these two modes.
32
2.Short = empty
1.Short = no step
Long = aspirate
1 2
Aspiration for
new task
Operation
Multipette
®
/Repeater ® (X)stream
English (EN)
4. Hold the Multipette stream/Repeater stream over a waste container or the supply
vessel and press the actuate key.
The reverse stroke is performed. This step is not a dispensing step.
5. Hold the Combitip over the target vessel. Press the actuate key to dispense the liquid.
After complete dispensing, the display shows:
6. There are two possible variants for continuing execution:
• Renewed aspiration
a Immerse the Combitip into the liquid to be dispensed.
b Press and hold the actuate key for 2 seconds to aspirate the liquid.
• Empty the Combitip completely and remove
a Hold the Multipette stream/Repeater stream over a tube to dispense the residual
liquid.
b Press the actuate key twice to empty the residual liquid.
c Remove the Combitip.
5.9 Titration
(only Multipette Xstream/Repeater Xstream)
In Ttr mode, liquid dispensing takes place
automatically with the actuate key held
down or gradually with the actuate key
pressed repeatedly. If the actuate key is
released, the piston movement stops and
the dispensed volume will be displayed.
When titration continues, the dispensing
speed is reduced automatically. The speed
is also reduced when the +/- rocker switch
is locked.
The speed can be changed during titration
by moving the select rocker switch and
changing the speed with the +/- rocker
switch.
1. Prepare the Multipette Xstream/Repeater Xstream.
2. Immerse the Combitip into the liquid to be aspirated.
3. Press the actuate key to aspirate the liquid into the Combitip until it is completely
filled. If less liquid is to be aspirated than the nominal volume of the Combitip, stop
aspiration with the actuate key.
For the first aspiration of liquid the display shows:
Multipette
next step
Discard
2.Short = empty
1.Short = no step
Long = aspirate
®
/Repeater ® (X)stream
Operation
English (EN)
The display will then show:
4. Hold the Multipette Xstream/Repeater Xstream over a waste container or the supply
vessel and press the actuate key.
The reverse stroke is performed. This step is not a dispensing step.
5. Hold the Combitip over the tube into which the liquid is to be titrated.
Start titration
6. Keep the actuate key pressed or press the key step by step to dispense the liquid.
If the actuate key is released, the piston movement stops and the display shows the
total of previously titrated volumes.
The speed decreases with each dispensing step. In this way you can reach the final
point of titration.
7. If the titration is completed before the Combitip is empty hold the Multipette Xstream/
Repeater Xstream over a waste container and press the reset key to empty the
Combitip.
After the emptying process is complete, the display shows the result of titration.
If the full volume of the Combitip is required the following will be shown on the
display:
8. There are two possible variants for continuing execution:
• Renewed aspiration
a Immerse the Combitip into the liquid to be aspirated.
b Press and hold the actuate key for 2 seconds to aspirate the liqued. You can
continue titration afterwards. The dispensing volumes are added to the
previously titrated volume.
The volume of at least 10 fillings of a Combitip can be displayed. An out-of-range
reading is indicated by "nnnnn".
• Empty the Combitip completely and remove
a Hold the Multipette Xstream/Repeater Xstream over a tube to dispense the
residual liquid.
b Press the actuate key twice to empty the residual liquid. The display then shows
the titrated volume.
c Remove the Combitip. The volume display is then extinguished.
The volume of the last correctly completed titration is saved and displayed in the
device settings (see p. 34).
33
34
Operation
Multipette
®
/Repeater ® (X)stream
English (EN)
5.10 Stopping or canceling pipetting operations
In an emergency you can cancel pipetting as follows:
During aspiration,
press the reset or actuate key. After a canceled aspiration operation the aspirated
partial volume can be dispensed.
During dispensing,
1. press the reset key.
Piston movement is stopped. After a stopped dispensing operation it is not possible to
correctly dispense the remaining volume.
2. Hold the Multipette stream/Repeater stream over an empty tube or the supply vessel.
3. Press the reset key again to dispense all of the liquid from the Combitip piston.
5.11 Adjusting the device settings
Under the device parameters you can make higher-order settings and view
supplementary information. The acoustic signals indicating errors cannot be deactivated.
The following settings and information are available:
Key tones BEEP Switch key tones on (ON) or off (OFF).
Brightness Light set the brightness level of the display.
Titration results Last
Software version display software version.
lock +/- rocker
switch
display the results of the last titration.
Ttr.
Only valid for the Multipette Xstream/Repeater Xstream.
It is also possible to display the last result if a new filling
operation of the Combitip was accidentally started in Ttr
mode. This is only possible if this operation is canceled
with the reset key at the latest when Discard next step
appears.
LOCK lock +/- rocker switch (ON) or unlock (OFF). When the +/
- rocker switch is locked, a locked padlock is shown in the
display. This option suitable for long dispensing series.
The parameter values can be viewed during dispensing.
Multipette
TIP:
Insert Combitip
Entry
system settings
Changed data
stored
®
/Repeater ® (X)stream
Operation
English (EN)
To access the device settings, proceed as follows:
1. If a Combitip is in the Multipette stream/Repeater stream: press ejector.
The display then shows:
2. Press both rocker switches up at the same time.
The following appears briefly in the display:
3. Select the parameter to be changed using the select rocker switch.
4. Change the setting of the selected parameter using the +/- rocker switch.
5. Press the actuate key to exit the menu.
When the settings have been successfully saved, the following appears briefly in the
display:
35
Troubles hooting
Warning
Battery Low!
Warning
Battery Low!
Battery empty
connect charger
Rocker switch
is locked
Multipette
36
®
/Repeater ® (X)stream
English (EN)
6Troubleshooting
6.1 Error search
Symptom/
Cause Remedy
message
Display:
One segment
empty.
Display:
One segment
Battery exhausted. 1. Finish dispensing.
2. Charge battery (see p. 20).
Battery exhausted. 1. Finish dispensing.
2. Charge battery (see p. 20).
empty.
Display: Battery severely discharged. 1. Confirm with the actuate key.
2. Charge battery (see p. 20).
3. Operation can be continued
with the power supply
connected.
Display: • Battery severely discharged. 1. Confirm with the actuate key.
2. Charge battery immediately
(see p. 20).
If the warning is repeatedly
ignored, the Multipette stream/
Repeater stream will be locked.
Battery empty Dispensing not possible.
Charge battery for at least
15 minutes.
Eppendorf recommends charging
the battery for at least 2 hours.
If you press the
+/- rocker
switch, the
following text is
displayed:
+/- rocker switch is locked. 1. Ensure that you are allowed to
unlock the +/- rocker switch.
2. Cancel the lock (see Adjusting
the device settings on p. 34).
During
operation, is
displayed.
Display is dark Multipette stream/Repeater stream
is switched off.
Press the actuate key to switch
on the Multipette stream/
Repeater stream.
Multipette
Press reset!
Attention!
Piston moves!
Troubleshooting
®
/Repeater ® (X)stream
English (EN)
37
Symptom/
Cause Remedy
message
• No battery in the Multipette
stream/Repeater stream.
• Battery is fully discharged
(battery is also discharged
when the device is not used).
Battery is defective.
Display switches
off during use
• Overcurrent protection has
been triggered.
• Combitip was ejected at the
wrong time.
• High backpressure (viscosity,
blockage) when dispensing.
Display: Reset key pressed while
dispensing.
Display flashes: • Ejector operated during
dispensing, causing the
Task aborted!
Press reset to
empty Combitip
Combitip cylinder to be
released.
• Cylinder pressed against the
wall too strongly or bent when
dispensing.
• A Combitip cylinder which was
released accidentally is not
inserted or inserted incorrectly.
Insert battery and charge it for
2 hours.
Insert a new battery and charge
it for 2 hours.
CAUTION!
The following steps will trigger a
piston movement and may cause
the dispensing of liquid!
1. If the Combitip has been
ejected at the wrong time:
carefully pull cylinder
downward by a few millimeters.
2. Trigger a hardware reset (see
p. 40).
3. Hold Combitip over the tube.
4. Press the reset key.
Caution!
The following action will trigger a
piston movement and the
dispensing of liquid.
1. Hold the Multipette stream/
Repeater stream over an empty
tube.
2. Press the reset key again.
1. Hold the Multipette stream/
Repeater stream over an empty
tube.
2. To empty the Combitip, press
the reset key again.
3. Press the ejector to remove the
Combitip.
4. Dispose of Combitip.
Troubles hooting
ERROR!
1. Adjust tip
2. press reset
Task stopped
Check cylinder
Press reset
Error volume
Piston stopped!
Press reset!
TIP:
Insert Combitip
Multipette
38
English (EN)
®
/Repeater ® (X)stream
Symptom/
Cause Remedy
message
Display flashes: • Combitip pressed against the
tube wall too strongly during or
ERROR!
1. Adjust tip
2. press reset
before dispensing.
• Ejector operated accidentally
and cylinder of the Combitip
released while the Combitip
was full.
Display flashes: The following error message was
cleared:
Error volume!
Use actuate key
to finish step
Display: • Step error during piston
movement.
•Piston movement was
obstructed.
Display: • Step error while aspirating or
dispensing liquid.
• Dispensing error.
• Combitip opening was closed
or viscosity too high.
Display: No Combitip used.
Do not use force to press the
inserted Combitip against the
tube wall.
If the cylinder has been released:
1. Carefully slide the cylinder of
the Combitip upward until it
locks into place.
2. Press the reset key. Further
information is displayed on
how to proceed.
1. Dispense the residual volume
into the affected tube.
2. Continue dispensing.
1. Remove obstruction.
2. If necessary: operate the
ejector to release the cylinder.
3. Pull the released cylinder
downward by a few millimeters.
4. Empty the Combitip and
remove (see p. 23).
1. Hold the Multipette stream/
Repeater stream over an empty
tube.
2. To empty the Combitip, press
the reset key.
3. In case of high viscosity: repeat
the dispensing operation at low
speed.
Insert the Combitip.
Multipette
Troubleshooting
®
/Repeater ® (X)stream
English (EN)
39
Symptom/
message
The filled
Combitip should
be emptied or
removed.
Combitip is
dripping.
Battery must be
charged very
often.
Further error messages displayed are intended only for error diagnosis by service
personnel. Contact your local Eppendorf partner if the error message cannot be cleared
by pressing the reset key or performing a hardware reset.
Avoiding dispensing errors
The precision and trueness of the Multipette stream/Repeater stream must be verified on
a regular basis. You can find a Standard Operating Procedure (SOP) for the Multipette
stream/Repeater stream on our homepage www.eppendorf.com
Cause Remedy
• The present residual liquid is
no longer required.
• A different Combitip should be
used.
• Combitip is not leak tight.
• Combitip is positioned
incorrectly.
• Charging capacity of the
battery has decreased.
• A large number of dispensing
operations has been performed.
1. Hold the Multipette stream/
Repeater stream over an empty
tube.
2. To empty the Combitip, press
the reset key.
3. Press the ejector to remove the
Combitip.
4. Dispose of Combitip.
Replace Combitip.
Observe the storage conditions.
If the charging capacity has
decreased:
1. Order a new rechargeable
lithium-ion battery.
2. Insert new rechargeable
lithium-ion battery.
3. Charge new rechargeable
lithium-ion battery for 2 hours
(see p. 20).
.
Troubles hooting
Multipette
40
English (EN)
®
/Repeater ® (X)stream
6.2 Pipette self-test
CAUTION! Injury to people and material damage from the release of sample
material when triggering the self test.
When the self test is triggered, the liquid is dispensed from the Combitip.
Hold the Multipette stream/Repeater stream over an empty container while
you trigger the self test.
The Multipette stream/Repeater stream can be tested as follows:
1. Make sure that the Combitip is in the base position or that no Combitip is inserted.
2. Hold Multipette stream/Repeater stream over an empty tube.
3. Press and hold the actuate key for 5 seconds.
The self-test begins and the display shows the software version of the Multipette
stream/Repeater stream.
If the self-test was successful, Check procedure OK is displayed.
6.3 Hardware reset
The hardware reset interrupts the power supply to the lithium-ion battery. Trigger a
hardware reset in emergencies only, for instance, after triggering an overcurrent
protection.
Without inserted Combitip
Press the tip of a 5 mL Combitip or a different pointy object onto the contact point in
the center of the selection dial.
With inserted Combitip
1. Press the tip of a 5 mL Combitip or a different pointy object onto the contact point in
the center of the selection dial.
2. Hold the Multipette stream/Repeater stream over a waste container.
3. Press the red reset key.
The piston of the Combitip is moved to the basic position.
After the reset, the software version is displayed for a few seconds.
Multipette
®
/Repeater ® (X)stream
Maintenance
English (EN)
7 Maintenance
7.1 Cleaning
NOTICE! Damage to device from unsuitable cleaning fluids or sharp or
pointed objects.
Unsuitable cleaning fluids can damage the device.
Never use corrosive cleaning fluids, strong solvents or abrasive polishes.
Check the compatibility with the materials used.
Please note the information on chemical resistance (see CD).
Do not clean the Multipette stream/Repeater stream with acetone or organic
solvents with a similar effect.
Do not clean theMultipette stream/Repeater stream with sharp objects.
NOTICE! Damage to the device due to penetration of liquids.
Do not allow any liquids to penetrate the inside of the housing.
If liquid has entered the inside of the housing, the inner parts may only be
repaired by Eppendorf AG service partners. Contact your responsible sales
office before returning a product.
Perform the following steps:
1. Carefully remove the contamination around the Combitip holder with a cotton bud.
2. Carefully wipe the housing of the Multipette stream/Repeater stream with a slightly
damp or dry cloth.
41
7.2 Maintenance
We recommend that you have all maintenance and servicing carried out by Eppendorf AG
or Eppendorf service partners. No warranty is provided for any damage due to misuse or
if the device has been opened by unauthorized persons.
Technical data
Multipette
42
®
/Repeater ® (X)stream
English (EN)
8 Technical data
8.1 Errors with the Multipette (X)stream/Repeater (X)stream
Combitip advanced Volume range Testing
volume
0.1 mL
white
Increment:
0.1 µL
0.2 mL
light blue
Increment:
0.2 µL
0.5 mL
purple
Increment:
0.5 µL
1 mL
yellow
Increment:
1 µL
2.5 mL
green
Increment:
2.5 µL
5 mL
blue
Increment:
5 µL
1 µL – 100 µL 10 µL 1.60 0.16 2.50 0.25
50 µL 1.00 0.50 1.50 0.75
100 µL 1.00 1.00 0.50 0.50
2 µL – 200 µL 20 µL 1.30 0.26 1.50 0.30
100 µL 1.00 1.00 1.00 1.00
200 µL 1.00 2.00 0.50 1.00
5 µL – 500 µL 50 µL 0.90 0.45 0.80 0.40
250 µL 0.90 2.25 0.50 1.25
500 µL 0.90 4.50 0.30 1.50
10 µL – 1000 µL 100 µL 0.90 0.90 0.55 0.55
500 µL 0.60 3.00 0.30 1.50
1 000 µL 0.60 6.00 0.20 2.00
25 µL – 2 500 µL 250 µL 0.80 2.00 0.45 1.125
1 250 µL 0.50 6.25 0.30 3.75
2 500 µL 0.50 12.5 0.15 3.75
50 µL – 5 000 µL 500 µL 0.80 4.00 0.35 1.75
2 500 µL 0.50 12.50 0.25 6.25
5 000 µL 0.50 25.00 0.15 7.50
Error limits
Error
Systematic error Random error
± % ± µL ± % ± µL
Multipette
®
Technical data
/Repeater ® (X)stream
English (EN)
43
Combitip advanced Volume range Testing
volume
Error limits
Error
Systematic error Random error
± %± mL± %± mL
10 mL
orange
Increment:
0.01 mL
25 mL
red
Increment:
0.025 mL
50 mL
light gray
Increment:
0.05 mL
0.1 mL – 10 mL 1 mL 0.50 0.005 0.25 0.0025
5 mL 0.40 0.02 0.25 0.0125
10 mL 0.40 0.04 0.15 0.015
0.25 mL – 25 mL 2.5 mL 0.30 0.0075 0.35 0.0088
12.5 mL 0.30 0.0375 0.25 0.0313
25 mL 0.30 0.075 0.15 0.0375
0.5 mL – 50 mL 5 mL 0.30 0.015 0.50 0.025
25 mL 0.30 0.075 0.20 0.05
50 mL 0.30 0.15 0.15 0.075
Test conditions and test analysis in accordance with ISO 8655, part 6. Test with an
analytical balance with a moisture trap which has been inspected by the Office of Weights
and Measures.
• Number of determinations: 10
• Use of water in accordance with ISO 3696
• Inspection at 20 °C – 25 °C ±0.5 °C
• Dispensing onto the tube wall
• Volume tests in the mode Dis
• Speed levels set: 7
The test volumes for the systematic and random error of the Multipette
(X)stream/Repeater (X)stream comply with the requirements of ISO 8655, part 5.
Technical data
Multipette
44
English (EN)
8.1.1 Battery
®
/Repeater ® (X)stream
Type Rechargeable lithium-ion battery
Overload protection in combination with the Multipette
stream/Repeater stream
Capacity 750 mAh / 3.7 V
Charging time Approx. 2 hours
Number of dispensings max. 2000 (if the battery is fully charged and with the
highest aspiration and dispensing speed)
Weight Approx. 32 g
8.1.2 Power supply
Type Power supply with insertable power plug adapter
Input voltage 100 V – 240 V; ± 10 %; 50/60 Hz
Output voltage 5 V / 1.0 A
8.1.3 Multipette stream/Repeater stream
Operating temperature +5 °C – +40 °C
Relative humidity during
20 % – 92 %
operation
Storage temperature -10 °C – +45 °C
Relative humidity during
10 % – 92 %
storage
Weight approx. 150 g (excluding battery and Combitip)
9 Ordering Information
9.1 Multipette stream/Repeater stream
Multipette
Ordering Information
®
/Repeater ® (X)stream
English (EN)
45
Order no.
(International)
4986 000.017
4986 000.025
–
–
Order no.
(International)
4986 900.115 4986900115 Operating Manual Multipette/Repeater (X)stream
4986 900.140 4986900140 Short instructions Multipette/Repeater (X)stream
Order no.
(North America)
–
–
022460803 Repeater stream
022460811 Repeater Xstream
Order no.
(North America)
Description
Multipette stream
Multipette Xstream
Description
9.2 Accessories
Charger stand 4880
For the Multipette stream/Repeater stream
Modified charger carousel 4880
For Xplorer pipettes and a Multipette
stream/Repeater stream
Ordering Information
Multipette
46
®
/Repeater ® (X)stream
English (EN)
A charger stand or charger carousel can be used for storing the Multipette stream/
Repeater stream and simultaneously charging the rechargeable Li-ion battery.
The power supply included in the accessories of the Multipette stream/Repeater stream
can be connected to the charger stand. The charger carousel is supplied with a more
powerful power supply.
The charger carousel 4880 is intended for Xplorer pipettes. To store and charge
the Multipette stream/Repeater stream in the charger carousel you need to order
a Multipette stream/Repeater stream charger shell and exchange it for an
Xplorer charger shell. The charger shell for a Multipette stream/Repeater stream
is partly colored in blue.
Order no.
(International)
Order no.
(North America)
Description
4880 000.018 4880000018 Charger stand 4880
for 1 Multipette/Repeater (X)stream
Charger carousel 4880
For 4 Xplorer, Multipette/Repeater (X)stream
4880 000.026 4880000026
Incl. power supply 4880 603.006
Charger shell
For charger carousel series 4880
4880 601.003 4880601003
Blue, for Multipette/Repeater (X)stream
Li-ion rechargeable battery
4986 602.009 022462407
for Multipette/Repeater (X)stream
4986 603.005 4986603005 Power supply with power plug adapters
Wall mount
4986 604.001 4986604001
for Multipette (X)stream/Repeater (X)stream
9.3 Combitips advanced
Order no.
(International)
Order no.
(North America)
Description
Combitips advanced 0.1 mL
100 pieces
0030 089.405
–
0030 089.618
0030 089.766
0030089405
0030089510
0030089618
–
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Multipette
Ordering Information
®
/Repeater ® (X)stream
English (EN)
47
Order no.
(International)
0030 089.413
–
0030 089.626
0030 089.774
0030 089.421
–
0030 089.634
0030 089.782
0030 089.430
–
0030 089.642
0030 089.790
0030 089.448
–
0030 089.650
0030 089.804
0030 089.456
–
0030 089.669
0030 089.812
0030 089.464
–
0030 089.677
0030 089.820
Order no.
(North America)
0030089413
0030089529
0030089626
–
0030089421
0030089537
0030089634
–
0030089430
0030089545
0030089642
–
0030089448
0030089553
0030089650
–
0030089456
0030089561
0030089669
–
0030089464
0030089570
0030089677
–
Description
Combitips advanced 0.2 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 0.5 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 1.0 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 2.5 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 5.0 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 10 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Ordering Information
Multipette
48
English (EN)
®
/Repeater ® (X)stream
Order no.
(International)
0030 089.472
–
0030 089.685
0030 089.839
0030 089.480
–
0030 089.693
0030 089.847
Order no.
(International)
0030 089.715 0030089715
0030 089.723 0030089723
0030 089.731 0030089731
0030 089.740 0030089740
Order no.
(North America)
0030089472
0030089588
0030089685
–
0030089480
0030089596
0030089693
–
Order no.
(North America)
Description
Combitips advanced 25 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 50 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Description
Adapter advanced 25 mL
1 piece
Eppendorf Quality
Adapter advanced 50 mL
1 piece
Eppendorf Quality
Adapter advanced 25 mL
7 pieces
Biopur, individually wrapped
Adapter advanced 50 mL
7 pieces
Biopur, individually wrapped
9.4 Accessories
Combitips ad
v
anced
®
Multipette
Ordering Information
®
/Repeater ® (X)stream
English (EN)
49
Combilong/Combitube
The Combilong/Combitube is an
aspiration tool for the Combitips
advanced. It enables liquids to be directly
taken out of all bottles.
Order no.
(International)
Order no.
(North America)
–
Description
Combilong
Aspirating aid for removing liquids from volumetric
flasks and tall bottles
0030 059.506
–
2 pieces
Combitube
Aspirating aid for removing liquids from volumetric
flasks and tall bottles
022261550
2 pieces
Combitips advanced Rack
1 piece
0030 089.758 0030089758
Eppendorf Quality
Combitips advanced Rack
A Combitips advanced Rack is available
for storing the Combitips advanced (≤ 10
mL).
Transport, storage and disposal
Multipette
50
English (EN)
®
/Repeater ® (X)stream
10 Transport, storage and disposal
10.1 Decontamination before shipment
If you are shipping the device to the authorized Technical Service for repairs or to your
authorized dealer for disposal please note the following:
WARNING! Risk to health from contaminated device
Please observe the information in the decontamination certificate, which is
available on our webpage as a PDF (www.eppendorf.com/decontamination
Decontaminate all the parts you would like to dispatch.
Include the fully completed decontamination certificate in the package.
10.2 Storage
NOTICE! Damage to device due to incorrect storage.
Remove the battery if you will not be using the Multipette stream/Repeater
stream for longer periods of time.
Do not clean the Multipette stream/Repeater stream while the Combitip is
inserted.
Select a secure storage location.
Do not expose the Multipette stream/Repeater stream to aggressive gases
over a longer period of time.
).
NOTICE! Damage due to UV radiation
Do not store consumables in areas with strong UV radiation.
In transport
packaging
Without transport
packaging
Air temperature Relative humidity Atmospheric
pressure
-25 °C – 55 °C 10 % – 95 % 70 kPa – 106 kPa
-5 °C – 45 °C 10 % – 95 % 70 kPa – 106 kPa
Transport, storage and disposal
Multipette
®
/Repeater ® (X)stream
English (EN)
10.3 Disposal
In case the product is to be disposed of, the relevant legal regulations are to be observed.
Information on the disposal of electrical and electronic devices in the European
Community:
Within the European Community, the disposal of electrical devices is regulated by
national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and
electronic equipment (WEEE).
According to these regulations, any devices supplied after August 13, 2005, in the
business-to-business sphere, to which this product is assigned, may no longer be
disposed of in municipal or domestic waste. They are marked with the following symbol to
indicate this:
As disposal regulations may differ from country to country within the
EU, please contact your supplier if necessary.
WARNING! Risk of explosion and fire due to overheated accumulators and
batteries.
Do not heat accumulators and batteries to over 80 °C and do not throw them
into fires.
51
Disposing of accumulators and batteries
Do not dispose of accumulators and batteries as household waste.
Dispose of accumulators and batteries according to the locally
applicable legal regulations.
52
Patents
Multipette
®
/Repeater ® (X)stream
English (EN)
11 Patents
U.S. Patent No. 6,499,365 Multipette stream/Xstream Repeater stream/Xstream
U.S. Patent No. 6,778,917 Multipette stream/Xstream Repeater stream/Xstream
U.S. Patent No. 7,585,468 Multipette stream/Xstream Repeater stream/Xstream
U.S. Patent No. 7,731,908 Multipette stream/Xstream Repeater stream/Xstream
U.S. Design Patent
384,163 Combitip plus 0.1 mL
No.
U.S. Design Patent
384,162 Combitip plus 2.5 mL
No.
U.S. Design Patent
387,426 Combitip plus 10 mL
No.
Evaluate your manual
Give us your feedback.
www.eppendorf.com/manualfeedback
Your local distributor: www.eppendorf.com/contact
Eppendorf AG · 22331 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com
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