5353 900.015
MixMate®
Operating manual
©
Copyright
2007 Eppendorf AG, Hamburg. No part of this publication may be reproduced without
the prior permission of the copyright owner.
Trademarks
eppendorf
®
and MixMate® are registered trademarks of Eppendorf AG, Hamburg, Germany.
Trademarks are not marked in all cases with ™ or
®
in this manual.
5353 900.015-11/102011
MixMate® — Operating manual
Operating manual — MixMate®seeon p.Fig.Tab.p.
Operating manual
Table of contents
1 Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Hazard icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.4 Abbreviations used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.5 Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.2 User profile. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.3 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
5.1 Overview of operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
5.2 Inserting plates and tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
5.2.1 Insert plate in the plate holder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
5.2.2 Insert the tube holder in the plate holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
5.2.3 Insert the plate in the PCR 96 tube holder . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.2.4 Insert tubes in the tube holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.3 Mixing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.3.1 Mixing with preset parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.3.2 Mixing with free preset parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.4 Vortexing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.4.1 Touch vortex mode with 3500 rpm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.4.2 Using free parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.5 Device menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.5.1 Menu structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.5.2 Menu navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.5.3 Key lock (LOCK) activation/deactivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.5.4 Adjusting signal tone volume (VOL) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
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Operating manual
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MixMate® — Operating manual
EN
6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
7.2 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7.3 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Operating manual
8.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
8.2 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
8.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
9.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
9.2 Ambient conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
9.3 Weight/dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
9.4 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
10 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
10.1 MixMate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
10.2 Tubes and plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
10.3 IsoTherm-System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
7.1.1 Cleaning the device and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
7.1.2 Performing a function test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4
MixMate® — Operating manual
1 Operating instructions
1 Operating instructions
1.1 Using this manual
Please read this operating manual completely before using the device for the first time. Also
observe the instructions for use enclosed with the accessories.
Please view this operating manual as part of the product and keep it somewhere easily
accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our
website www.eppendorf.com
1.2 Danger symbols and danger levels
1.2.1 Hazard icons
Explosion Electric shock
Hazard point Material damage
1.2.2 Degrees of danger
The safety instructions in this operating manual indicate the following degrees of danger:
DANGER Will lead to severe injuries or death.
WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
.
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Operating manual
NOTICE May lead to material damage.
1.3 Symbols used
Depiction Meaning
You are requested to perform an action.
1.
2.
Perform these actions in the sequence described.
• List.
Text Terms and key names from the software.
References useful information.
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MixMate® — Operating manual
EN
1.4 Abbreviations used
ANSI American National Standards Institute
DNA Deoxyribonucleic acid
DWP Deepwell plate
MTP Microplate
PCR Polymerase Chain Reaction
RNA Ribonucleic acid
rpm revolutions per minute
SBS Society for Biomolecular Screening
Operating manual
1.5 Glossary
Deepwell plate Plate with 48, 96 or 384 wells with a larger volume than
Incubate Includes: cultivation of cell and bacterial cultures under
Micro test plate Plates with 24, 48, 96 or 384 wells for the preparation,
Mixing load All samples to be mixed and the tubes or plates in which
Pellet Compressed material. Is, for example, created via the
Resuspending Dissolve the pellet by vortexing in a liquid. The material
Semi-skirted PCR
plate
Skirted PCR plate PCR plate with a surrounding edge.
microplates. Suitable for the preparation, mixing,
centrifuging, transporting and storing of solid and liquid
samples.
controlled ambient conditions.
mixing, centrifuging, transporting and storing of solid
and liquid samples.
the samples are located.
centrifugation of a suspension.
is distributed in the liquid. The result is a suspension.
PCR plate with surrounding half-edge.
Unskirted PCR plate PCR plate without a surrounding edge.
Vortexing Strong whirling or blending by manually pressing a tube
Well Cavity. Microplate, PCR plate or Deepwell plate tube.
6
onto the vortex mat.
MixMate® — Operating manual
2 Product description
2 Product description
2.1 Main illustration
Abb. 1:Front and rear view
Fig. 1: Front and rear view
1 Plate holder
For holding skirted PCR plates, MTP and
DWP as well as tube holders.
3Softkeys
For selecting preset mixing parameters.
5 Operating controls
Keys for operating the MixMate (Fig. 3 on
p. 12).
7 Power connection socket
Connection for the supplied power cable.
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Operating manual
2Vortex mat
For direct vortexing of various tubes.
4Display
Displays the mixing frequency and the
mixing time (Fig. 4 on p. 12).
6 Mains power switch
Switch for switching the device on and off.
Switch position 0: the device is switched
off. Switch position I: the device is switched
on.
Abb. 2:Tube holder for the MixMate
Fig. 2: Tube holder for the MixMate
1 PCR 96 tube holder
For one PCR plate (96-well, semi-skirted or
unskirted) or a max. of 96 tubes (0.2 mL).
3 1.5/2.0 mL tube holder
For max. 24 tubes (1.5 and 2.0 mL).
2 0.5 mL tube holder
For max. 24 tubes (0.5 mL).
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MixMate® — Operating manual
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2.2 Delivery package
Quantity Order No.
1
or
1
1
Operating manual
1
1- - Power cable
1 5353 900.015
1 5353 900.023 Short Instructions MixMate
2.3 Features
The MixMate allows aqueous solutions and suspensions to be mixed and vortexed effectively in
a wide range of micro test tubes or plates. The MixMate supports from 0.2 mL PCR tubes to 2.0
mL micro test tubes as well as PCR, MTP and DWP plates up to 384 wells at a maximum mixing
frequency of up to 3000 rpm.
The direct select buttons facilitate rapid access to selected mixing parameters (see p. 15).
Potential applications include:
• controlled mixing of PCR, restriction or other enzyme reactions.
• controlled incubation of absorption, blocking or reaction preparations.
• resuspension of DNA, RNA, protein or cell pellets in tubes and in plates.
• vortexing in micro test tubes and in 15 mL/50 mL screw-top tubes.
(International)
5353 000.014
5353 000.022
5353 040.113
5353 040.121
5353 040.130
Order No.
(North
America)
022674226
022674200
022674005
022674021
022674048
Description
MixMate
230 V
120 V
Tube Holder
PCR 96
0.5 mL
1.5/2.0 mL
Operating Manual MixMate
multi-lingual
3 Safety
3Safety
3.1 Intended use
Exclusively intended for use indoors, the MixMate is used for mixing aqueous solutions and
suspensions in closed tubes and closed plates.
Only use Eppendorf accessories or accessories recommended by Eppendorf.
3.2 User profile
This device may only be operated by trained and skilled personnel.
Before using the device, read the operating manual carefully and familiarize yourself with the
device's mode of operation.
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MixMate® — Operating manual
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised. Liability for
material damage and personal injury is transferred to the operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables not recommended by Eppendorf.
• The device is maintained or repaired by persons not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
3.4 Warnings for intended use
Read the operating instructions and observe the following general safety information before
using the MixMate.
DANGER! Danger of explosion!
Do not operate the device in areas where work is completed with explosive
DANGER!
substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which could create an explosive
atmosphere.
WARNING! Electric shock due to damage to device or mains cable.
Only switch on the device if the device and mains cable are undamaged.
WARNING!
Only use devices that have been properly installed or repaired.
In case of danger, disconnect the device from the mains supply.
WARNING! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside
WARNING!
the device can be contacted by accident.
Do not remove the housing of the device.
Do not allow any liquids to penetrate the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who have
been specifically authorized by Eppendorf.
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Operating manual
WARNING! Device fire due to penetration of liquid
Penetration of liquid can cause a fire due to a short-circuit in the device.
WARNING!
Do not allow any liquids to penetrate the inside of the housing.
Only mix in closed tubes and closed plates.
If liquid has penetrated the inside of the housing: switch off the device, pull the
power plug and have the device cleaned by service personnel authorized by
Eppendorf.
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MixMate® — Operating manual
EN
WARNING!
Operating manual
WARNING!
WARNING!
WARNING! Injury from flying tubes and plates.
If the maximum permitted total weight of the mixing load is exceeded, plates or tubes
may become detached from the device.
Always ensure that tubes, plates and tube holder are well seated. The dimensions
of the plates used must comply with the ANSI/SBS standards for microplates
Mix the DWP and the tube holders 0.5 mL, 1.5/2.0 mL and PCR 96 with max. 2000
rpm.
Only mix mixing loads with a total weight of up to 80 g at maximum speed.
Only mix mixing loads with a total weight of 80 to 300g at speed of max. 2000 rpm.
WARNING! Injury from sample material being thrown out.
Sample material can be thrown out of open, improperly sealed or unstable tubes and
plates.
Only mix in closed tubes and closed plates.
Observe the nationally prescribed safety environment when working with
hazardous, toxic and pathogenic samples. Pay particular attention to the personal
protective equipment (gloves, clothing, goggles etc.), extraction, and the safety
class of the lab.
WARNING! Injury from improper vortex action.
Improper vortex action can destroy tubes or cause their content to be lost.
Only vortex intact and sealed tubes.
Never vortex tubes made of glass or other fragile material.
10
WARNING! Risk from incorrect supply voltage
Only connect the device to power supplies which correspond with the electrical
WARNING!
requirements on the nameplate.
Only use sockets with a protective earth (PE) conductor and a suitable mains cable.
NOTICE! Damage to the display due to mechanical pressure.
Do not exert mechanical pressure on the display.
NOTICE!
NOTICE! Caution! Strong vibration.
When mixing at high speeds, items located near the device may be moved by the
NOTICE!
vibrations of the work surface and, e.g., fall off the work table.
Do not place easily movable items near the mixer or secure them adequately.
NOTICE! Damage to electronic components from spilled liquids.
Make sure that the vortex mat and the cover caps are fitted properly. If the vortex
NOTICE!
mat is not fitted properly, contact your Eppendorf partner or the authorized
Technical Service.
If liquid has been spilt: Switch off the device, disconnect the power plug and
arrange for it to be cleaned by service personnel authorized by Eppendorf.
MixMate® — Operating manual
NOTICE! Damage to electronic components due to condensation.
After the device has been moved from a cool to a warmer environment, condensation
NOTICE!
may form inside the device.
Wait at least three hours before connecting it to the power supply.
NOTICE! Damage from the use of aggressive chemicals.
Do not use any aggressive chemicals on the device or its accessories, such as
NOTICE!
4 Installation
4 Installation
4.1 Preparing installation
Hint!
Use the details on the scope of delivery (see Delivery package on p. 8) to check that delivery
is complete.
Check all parts for any transport damage.
strong and weak bases, strong acids, acetone, formaldehyde, halogenated
hydrocarbons or phenol.
If the device becomes contaminated with aggressive chemicals, clean it
immediately with a mild cleaning agent.
Keep the transport carton and the packing material for subsequent safe transport or
storage.
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Operating manual
4.2 Selecting the location
Select a location for the MixMate in accordance with the following criteria:
• Mains power connection (230 V/120 V) as per device identification plate. This is located on
the rear side of the device.
• At least 10 cm away from adjacent devices and walls.
• Solid bench with stable, horizontal and even work surface.
4.3 Installing the instrument
1. Place theMixMate on a suitable work surface so that the air slots on the underside of the
device are not blocked.
2. Connect the device to the mains power supply via the mains power socket 7 (see Fig. 1 on
p. 7) using the mains cable supplied.
3. Switch on the device with the mains power switch 6 (see Fig. 1 on p. 7).
4. Carry out a test run at maximum speed (3000 rpm) to ensure that the grip between the
device and the surface is sufficient. The MixMate must not move from its position.
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MixMate® — Operating manual
EN
5Operation
5 Operation
5.1 Overview of operating controls
Familiarize yourself with the operating controls and the display of the MixMate before using it for
the first time.
Abb. 3:Operator control elements and displ ay
Operating manual
Fig. 3: Operator control elements and display
1 Soft key for MTP (384-well) 2 Soft key for PCR plates (384-well)
3 Soft key for MTP (96-well) 4 Soft key for PCR plates (96-well) and
5 Softkey for micro test tubes (1.5 and
2.0 mL)
7 Display 8 Start/stop mixing run
9 Set the mixing frequency (speed) 10 Set the mixing duration (time)
11 Navigate in the menu 12 Call up and select menu parameters
Abb. 4:Display
micro test tubes (0.2 and 0.5 mL)
6 Control LED to display the selected
softkey
Fig. 4: Display
1 Mixing time
Setting: up to 19:45 min. in 15 s
increments, from 20 min. to 59 min. in 1
min. increments, from 1.0 h to 99.5 h in 0.5
h increments, 'oo': unlimited mixing time.
3 Mixing frequency
Setting: 300 to 3000 rpm in 50 rpm
increments.
After switching on, the display shows the values of the last run.
Hint!
12
2 Symbol for key lock
4 Symbol for signal tone setting
MixMate® — Operating manual
5.2 Inserting plates and tubes
WARNING! Injury from flying tubes and plates.
If the maximum permitted total weight of the mixing load is exceeded, plates or tubes
WARNING!
may become detached from the device.
Always ensure that tubes, plates and tube holder are well seated. The dimensions
of the plates used must comply with the ANSI/SBS standards for microplates
Mix the DWP and the tube holders 0.5 mL, 1.5/2.0 mL and PCR 96 with max. 2000
rpm.
Only mix mixing loads with a total weight of up to 80 g at maximum speed.
Only mix mixing loads with a total weight of 80 to 300g at speed of max. 2000 rpm.
Tab. 1: Select a suitable holder
Plate / tube
PCR plate, skirted +
PCR plate, semi-skirted +
PCR plate, unskirted +
MTP +
(2)
DWP
PCR tubes 0.2 mL +
Plate holder
(1)
+
PCR 96 0.5 mL 1.5/2.0 mL
Tube holder
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Operating manual
(2)
PCR and tubes 0.5 mL +
Tubes 0.5 mL +
Tubes 1.5 mL +
Tubes 2.0 mL +
(1) To ensure that the plates are positioned securely in the plate holder, they must correspond
with the ANSI/SBS Standard for microplates.
(2) The maximum permissible mixing frequency for tube holders and DWP is 2000 rpm.
5.2.1 Insert plate in the plate holder
1. Place the plate up against the back of the plate holder 1 first.
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MixMate® — Operating manual
EN
2. Then press the plate into the plate holder. In doing so, ensure that it is properly engaged.
Operating manual
5.2.2 Insert the tube holder in the plate holder
1. Select a suitable tube holder (see Tab. on p. 13).
2. Hold the tube holder up against the back edge of the plate holder so that the stop pins fit in
the holes:
3. Engage the tube holder by pressing gently on the front.
Remove the tube holder by pressing gently on the unlatching Push key to release the latch.
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MixMate® — Operating manual
5.2.3 Insert the plate in the PCR 96 tube holder
1. Insert the PCR 96 tube holder in the plate holder (see Insert the tube holder in the plate
holder on p. 14).
2. Push semi-skirted or unskirted PCR plates into the bores of the tube holder. In doing so,
ensure they are evenly engaged.
5.2.4 Insert tubes in the tube holders
1. Select the suitable tube holder (Tab. on p. 13).
2. Insert it into the plate holder (see Insert the tube holder in the plate holder on p. 14).
3. Push the tubes fully into the bores of the tube holder.
5.3 Mixing
WARNING! Injury from sample material being thrown out.
Sample material can be thrown out of open, improperly sealed or unstable tubes and
WARNING!
plates.
Only mix in closed tubes and closed plates.
Observe the nationally prescribed safety environment when working with
hazardous, toxic and pathogenic samples. Pay particular attention to the personal
protective equipment (gloves, clothing, goggles etc.), extraction, and the safety
class of the lab.
WARNING! Device fire due to penetration of liquid
Penetration of liquid can cause a fire due to a short-circuit in the device.
WARNING!
Do not allow any liquids to penetrate the inside of the housing.
Only mix in closed tubes and closed plates.
If liquid has penetrated the inside of the housing: switch off the device, pull the
power plug and have the device cleaned by service personnel authorized by
Eppendorf.
EN
Operating manual
Note the following rule when setting the mixing frequency: only mix at a load above 80 g
Hint!
with a maximum mixing frequency of 2000 rpm.
The MixMate comes with an automatic protection against overloading. If the selected
speed for the mixing load is too high or the mixing load is not securely placed on the
plate holder a signal tone sounds. The MixMate reduces the mixing frequency
automatically to 1400 rpm. In the display the messages TOO FA S T and 1400 rpm
appear alternately.
Press start/stop to end the mixing process. Press again start/stop to deactivate the
error message.
5.3.1 Mixing with preset parameters
With the softkey you can select the following preset parameters (mixing frequency and mixing
duration). These permit a controlled and effective mixing of samples without wetting the tube lids
or plate sealings. The softkey cannot be programmed.
15
MixMate® — Operating manual
EN
Tab. 2: Softkeys for preset, optimized mixing parameters
Softkey Parameter Tube/ plate Fill level
Operating manual
* Amount of maximum filling volume. Follow the manufacturer instructions.
Hint!
1. Select the appropriate parameters from the table (see Tab. on p. 16).
2. Select the respective softkey.
3. If necessary change the preset mixing duration and mixing frequency with the arrow keys
time and speed.
If you change the parameter settings the indicator lamp of the softkey goes out.
4. Press start/stop to start the mixing process.
At the end of the mixing process a signal tone sounds.
Changed parameters are not saved. At the end of the mixing process the original parameters
are reassigned to the softkeys.
5.3.2 Mixing with free preset parameters
*
15 s/2000 rpm MTP and DWP (384 well) 10 to 60 %
15 s/2600 rpm PCR plates (384 well) 10 to 50 %
30 s/1000 rpm MTP (96 well) 5 to 60 %
30 s/1650 rpm PCR plates and DWP (96
well), PCR tubes (0.2 mL) and
micro test tubes (0.5 mL)
1 min/1400 rpm Micro test tubes (1.5 and 2.0
mL)
The parameters of the softkeys do not cover all known or possible of tube or plate
geometry as well as sample properties.
For some applications these parameters can possibly be optimized. For example, the
mixing frequency can be too low or the mixing duration can be too short. As a result the
samples are not optimally mixed. Or the mixing frequency is too high which leads to the
wetting of the lid. In that case you can adjust the preset parameters before stating the
mixing procedure to your requirements.
5 to 50 %
5 to 80 %
After switching on the device, the parameters of the last run are shown on the display.
Hint!
1. Set the with the mixing time using the time arrow keys.
For continuous operation set oo below 0:15 min or above 99.5 h.
2. Set the with the mixing frequency using the speed arrow keys.
3. Press start/stop to start the mixing process.
16
MixMate® — Operating manual
The remaining mixing duration and the current mixing frequency are displayed. At continuous
operation the current mixing duration is displayed alternating with oo, after 99.5 h only oo is
displayed.
You can also change the parameters during a mixing process by pressing the time and
speed arrow keys.To do so, the key lock may not be active (see Key lock (LOCK) activation/
deactivation on p. 20). The mixing procedure is then continued with the changed parameters.
The softkeys are not available during a mixing procedure.
At the end of the mixing process a signal tone sounds. The MixMate stops.
4. In order to stop the mixing process, if applicable, prematurely press start/stop again.
5.4 Vortexing
During the vortexing process, press different tubes (e.g., 1.5 mL tubes or 50 mL screw cap tubes)
on the vortex mat of the MixMate to mix them individually.
WARNING! Injury from improper vortex action.
Improper vortex action can destroy tubes or cause their content to be lost.
WARNING!
Only vortex intact and sealed tubes.
Never vortex tubes made of glass or other fragile material.
NOTICE! Damage to vortex mat caused by improper vortexing.
Only vortex tubes in the depression in the middle of the vortex mat.
NOTICE!
5.4.1 Touch vortex mode with 3500 rpm
With the touch vortex mode, vortexing is carried out at a fixed frequency of 3500 rpm.
1. Push the tube into the depression in the vortex mat to start the touch vortex mode.
In the display, the VORTX mode is shown, along with the elapsed time:
• Up to 1 min in second increments.
• Up to 19:59 h in minute increments.
• The time display then changes to oo.
2. Remove the load from the vortex mat in order to end the touch vortex mode.
The MixMate continues to run for approx. another 2 s.
EN
Operating manual
Ergonomic vortexing: A post-run facility in touch vortex mode prevents the MixMate
Hint!
braking immediately after the load is removed from the vortex mat. This post-run
facilitates both vortexing with 15 and 50 mL screw cap tubes and vortexing several
tubes consecutively.
17
MixMate® — Operating manual
EN
5.4.2 Using free parameters
You can also vortex on the vortex mat of the MixMate using free parameters. Vortexing time can
be set variably from 15 s to infinite, vortexing frequency from 300 to 2000 rpm.
Hint!
Operating manual
1. Set the mixing time using the time arrow keys.
For continuous operation set oo below 0:15 min or above 99.5 h.
2. Set the with the mixing frequency using the speed arrow keys.
3. Press start/stop to start the vortexing process.
4. Hold the tube on the vortex mat.
The remaining vortexing time and the current vortexing frequency are displayed. During
continuous operation, the current vortexing time appears alternately with oo, after 99.5 h only
oo is displayed.
You can also change the parameters during the vortexing process with the arrow keys time
and speed.To do so, the key lock may not be active (see Key lock (LOCK) activation/
deactivation on p. 20). The vortexing process is then continued with the changed
parameters.
The touch vortex mode is not active during the vortexing process.
A signal tone is generated at the end of the vortexing process. The MixMate stops.
5. In order to cancel the vortexing process prematurely, press start/stop again.
5.5 Device menu
In the MixMate menu, you can activate the key lock (LOCK) and set the volume of the signal
tone (VOL) .
In this Vortex mode, the TOO FAST message may be issued at frequencies > 2000
rpm. The MixMate automatically reduces the frequency to 1400 rpm. In the display the
messages TOO FA S T and 1400 rpm appear alternately.
Press the start/stop button to end the vortexing process. Press again start/stop to
deactivate the error message.
The touch vortex mode is inactive as long as the MixMate is running.
18
MixMate® — Operating manual
5.5.1 Menu structure
Abb. 5:Menu structure
EN
Operating manual
Fig. 5: Menu structure
5.5.2 Menu navigation
With the menu/enter button, you can switch between the menu levels or confirm the
changed parameters.
You can select the parameters with the arrow key 11.
When M – 1 BACK or M – 2 BACK is displayed, press the menu/enter button to exit the
menu level without saving changes.
19
MixMate® — Operating manual
EN
5.5.3 Key lock (LOCK) activation/deactivation
The key lock prevents set parameters being modified inadvertently during a mixing operation.
1. Press the menu/enter button to call up the menu.
2. Press the menu arrow key once.
3. Press the menu/enter button to open the key lock menu.
4. Select sub-item M – 2 ON with the menu arrow key to activate the key lock, or M – 2 OFF to
deactivate the key lock.
5. Press the menu/enter button to confirm the selected setting.
You will then exit menu level 2.
Operating manual
Press the menu/enter button again to exit the menu completely.
With key lock activated, all keys apart from menu/enter are inactive during the mixing operation.
When the mixer is at rest, all the keys are released.
You can tell from the symbol in the display that key lock is active and from the symbol that
key lock is deactivated.
5.5.4 Adjusting signal tone volume (VOL)
The MixMate announces that a mixing operation is complete by means of a signal tone. You can
set the volume of this signal tone in the device menu as follows -
1. Press the menu/enter button to call up the menu.
2. Press the menu arrow key twice.
3. Press the menu/enter button to open the menu for the signal tone volume.
4. Use the menu arrow key to select the desired setting from OFF, VOL1 to VOL3. With OFF
the signal tone is switched off, with VOL3 the signal tone sounds with maximum volume.
The selected volume is played.
5. Press the menu/enter button to confirm the selected setting.
You will then exit menu level 2.
Press the menu/enter button again to exit the menu completely.
6. Press M – 2 BACK to leave this menu level.
6 Troubleshooting
6 Troubleshooting
6.1 General errors
If the suggested troubleshooting measures fail repeatedly, please contact your Eppendorf
partner. You can find the contact addresses on the Internet at www.eppendorf.com/worldwide
Symptom/
message
No display Power supply is interrupted.
Too fast Mixing load is too heavy for the
20
Cause Remedy
Check the mains connection
and the power supply to the
lab.
Reduce the mixing frequency
selected mixing frequency.
or the weight of the mixing load.
.
MixMate® — Operating manual
Symptom/
message
Too fast Mixing load not properly positioned
Too fast Continuous vortexing at
ERR00 - ERR03 /
ERR06 - ERR11
ERR04 - ERR05 Device overheats.
ERR12 Incorrect power supply
ERR13 Software error
ERR15 and ERR16 Hardware error
Cause Remedy
in the plate holder.
frequencies > 2000 rpm.
Electronics error
The ventilation slots on the
underside of the device are
blocked.
Check that the mixing load is
fixed in position.
Reduce the continuous
vortexing frequency to
≤ 2000 rpm.
Switch off the device and
switch it on again after 5 s.
1. Switch off the device and leave
to cool down for 10 minutes.
2. Ensure that the ventilation slots
on the underside of the device
are clear.
3. Switch the device back on
again.
Make sure that the supply
voltage and the power
frequency are compatible with
the information given on the
device name plate. This is
located on the underside of the
device.
Switch off the device and
switch it on again after 5 s.
Contact your Eppendorf
partner.
EN
Operating manual
7 Maintenance
7 Maintenance
7.1 Cleaning
7.1.1 Cleaning the device and accessories
Clean the housing of the MixMate, the vortex mat, the plate holder and the tube holders regularly.
DANGER! Electric shock as a result of penetration of liquid.
Switch off the device and disconnect it from the power supply before starting
DANGER!
cleaning or disinfecting.
Do not allow any liquids to penetrate the inside of the housing.
Do not spray clean/spray disinfect the housing.
Wait until the device is completely dry before connecting it to the power source
again.
21
EN
MixMate® — Operating manual
WARNING! Device fire due to penetration of liquid
Penetration of liquid can cause a fire due to a short-circuit in the device.
WARNING!
Do not allow any liquids to penetrate the inside of the housing.
Only mix in closed tubes and closed plates.
If liquid has penetrated the inside of the housing: switch off the device, pull the
power plug and have the device cleaned by service personnel authorized by
Eppendorf.
Operating manual
NOTICE! Damage from the use of aggressive chemicals.
Do not use any aggressive chemicals on the device or its accessories, such as
NOTICE!
strong and weak bases, strong acids, acetone, formaldehyde, halogenated
hydrocarbons or phenol.
If the device becomes contaminated with aggressive chemicals, clean it
immediately with a mild cleaning agent.
NOTICE! Corrosion due to aggressive cleaning agents and disinfectants.
Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes.
NOTICE!
Do not incubate the accessories in aggressive cleaning agents or disinfectants for
prolonged periods.
NOTICE! Damage to electronic components from spilled liquids.
Make sure that the vortex mat and the cover caps are fitted properly. If the vortex
NOTICE!
mat is not fitted properly, contact your Eppendorf partner or the authorized
Technical Service.
If liquid has been spilt: Switch off the device, disconnect the power plug and
arrange for it to be cleaned by service personnel authorized by Eppendorf.
Required equipment
• Mild, soap-based household cleaning agent
1. Switch off the MixMate and isolate it from the power supply.
2. Clean the housing, plate holder, vortex mat and tube holder.
The housing may only be wiped with a damp cloth. Do not spray clean/spray disinfect the
housing.
3. Dry all cleaned parts.
4. Perform a function test.
7.1.2 Performing a function test
1. Use the mains cable to connect the MixMate to the power supply (see Installing the
instrument on p. 11).
2. Switch on the device, using the mains power switch.
3. Check the touch vortex function, using a suitable tube (see Vortexing on p. 17).
22
MixMate® — Operating manual
7.2 Disinfection/decontamination
DANGER! Electric shock as a result of penetration of liquid.
Switch off the device and disconnect it from the power supply before starting
DANGER!
cleaning or disinfecting work.
Do not allow any liquids to penetrate the inside of the housing.
Do not spray clean/spray disinfect the housing.
Only connect the device to the power supply if it is fully dried inside and out.
WARNING! Device fire due to penetration of liquid
Penetration of liquid can cause a fire due to a short-circuit in the device.
WARNING!
Do not allow any liquids to penetrate the inside of the housing.
Only mix in closed tubes and closed plates.
If liquid has penetrated the inside of the housing: switch off the device, pull the
power plug and have the device cleaned by service personnel authorized by
Eppendorf.
NOTICE! Damage from UV and other high-energy radiation.
Do not use UV, beta, gamma, or any other high-energy radiation for disinfecting.
NOTICE!
Required equipment
• Alcohol (ethanol, isopropanol) or disinfectants containing alcohol
• Mild, soap-based household cleaning agent
Proceed as follows:
1. Choose the disinfection method which corresponds to the legal regulations and guidelines in
place for your range of application.
2. Switch off the device and disconnect it from the power supply.
3. Wipe down all parts of the device and accessories, including the connecting cable, with the
disinfectant.
4. Clean the device with a mild soap-based household cleaning agent (see Cleaning on p. 21).
EN
Operating manual
7.3 Decontamination before shipment
If you are shipping the device to the authorized Technical Service for repairs or to your authorized
dealer for disposal please note the following:
WARNING! Risk to health from contaminated device
WARNING!
1. Follow the instructions in the decontamination certificate. It is available in PDF
format on our homepage (www.eppendorf.com/decontamination)
2. Decontaminate all the parts you want to dispatch.
3. Enclose the fully-completed decontamination certificate for returned goods
(including the serial number of the device) with the dispatch.
.
23
MixMate® — Operating manual
EN
8 Transport, storage and disposal
8 Transport, storage and disposal
8.1 Transport
Only transport the device in the original packaging.
General
transportation
Air freight -20 to 55 °C 10 to 95 % 30 to 106 kPa
Operating manual
8.2 Storage
in transport packaging -20 to 55 °C 10 to 95 % 70 to 106 kPa
without transport
packaging
8.3 Disposal
In case the product is to be disposed of, the relevant legal regulations are to be observed.
Information on the disposal of electrical and electronic devices in the European
Community:
Within the European Community, the disposal of electrical devices is regulated by national
regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic
equipment (WEEE).
According to these regulations, any devices supplied after August 13, 2005, in the
business-to-business sphere, to which this product is assigned, may no longer be disposed of in
municipal or domestic waste. To document this, they have been marked with the following
identification:
Air temperature Max. rel. humidity Air pressure
-20 to 60 °C 10 to 95 % 30 to 106 kPa
Air temperature Max. rel. humidity Air pressure
-5 to 45 °C 10 to 95 % 70 to 106 kPa
Because disposal regulations may differ from one country to another within the EU, please
contact your supplier if necessary.
24
MixMate® — Operating manual
9 Technical data
9 Technical data
9.1 Power supply
Mains power connection: 220 to 240 V ±10 %, 50 to 60 Hz
110 to 120 V ±10 %, 50 to 60 Hz
Power consumption: 40 W
Overvoltage category: II
9.2 Ambient conditions
Environment: Use only indoors
Ambient temperature: 2 to 40 °C
Relative humidity: 10 to 75 %
Atmospheric pressure: Use up to an altitude of 2000 m above MSL.
Degree of contamination: 2
9.3 Weight/dimensions
Dimensions: Width: 170 mm
Depth: 230 mm
Height: 130 mm
Weight: 4.15 kg
Noise level: < 50 dB(A)
9.4 Application parameters
EN
Operating manual
Max. load: 300 g
Mixing frequencies
with a load up to 80g: 300 to 3000 rpm, in 50 rpm increments
with a load above 80 g: 300 to 2000 rpm, in 50 rpm increments
for tube holders and DWP: up to max. 2000 rpm
Adjustable mixing time: up to 19:45 min. in 15 s increments,
from 20 min. to 59 min. in 1 min. increments,
from 1.0 h to 99.5 h in 0.5 h increments
and unlimited mixing time.
Touch vortexing frequency: 3500 rpm
Mixing and vortexing radius: 1.5 mm (3 mm mixing stroke)
25
MixMate® — Operating manual
EN
10 Ordering Information
10 Ordering Information
CAUTION!
Operating manual
10.1 MixMate
Order No.
(International)
5353 000.014
5353 000.022
5353 040.113
5353 040.121
5353 040.130
5353 863.101 5353863101
10.2 Tubes and plates
Order No.
(International)
0030 121.023 022363611
0030 120.086 -
0030 120.094 -
0030 124.502 951010057
0030 124.332 951010006
0030 124.340 951010014
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf
may impair the safety, function and precision of the device. Eppendorf cannot be held
liable or accept any liability for damage resulting from the use of incorrect or
non-recommended accessories and spare parts or from the improper use of such
equipment.
Only use accessories and original spare parts recommended by Eppendorf.
Order No.
(North America)
022674226
022674200
022674005
022674021
022674048
Order No.
(North America)
Description
MixMate
230 V
120 V
Tube Holder
PCR 96
0.5 mL
1.5/2.0 mL
Cover caps
set of 4
Description
Safe-Lock micro test tubes
0.5 mL per 500 pieces
colorless
Safe-Lock micro test tubes
1.5 mL per 1,000 pieces
colorless
Safe-Lock micro test tubes
2.0 mL per 1,000 pieces
colorless
PCR tubes
thin-walled with hinged lid, colorless, 500 pieces
0.5 mL
PCR Tubes
0.2 mL, colorless, 1,000 pieces
Five-tube strip for 0.2 mL PCR Tubes
colorless, pack of 125 (= 625 tubes)
26
MixMate® — Operating manual
Order No.
(International)
0030 124.359 951010022
0030 124.200 951010006
0030 128.648
0030 128.575
0030 521.102 951031003
0030 501.101 951031801
0030 501.209 951032603
0030 501.306 951033405
All plates are also available with different border colors (red, yellow, green and blue) and purity
qualities, in large packs as well as with barcoding on request. You can find further information in
our catalog or our website www.eppendorf.com
Order No.
(North America)
951020401
951020303
Description
PCR Tube Strips
0.2 mL, colorless, 120 strips
PCR Tubes
0.2 mL per 1,000 pieces
colorless
twin.tec PCR Plate 96
Wells colorless
skirted, colorless, 25 pcs.
semi-skirted, colorless, 25 pcs.
Eppendorf Deepwell Plate 384/200 μL
40 plates, wells clear
PCR Clean, white border color
Eppendorf Deepwell Plate 96/500 μL
40 plates, wells clear
PCR Clean, white border color
Eppendorf Deepwell Plate 96/1000 μL
20 plates, wells clear
PCR Clean, white border color
Eppendorf Deepwell Plate 96/2000 μL
20 plates, wells clear
PCR Clean, white border color
.
10.3 IsoTherm-System
EN
Operating manual
Order No.
(International)
3880 001.018
3880 000.011
3881 000.015
3881 000.023
3881 000.031
Order No.
(North America)
022510053
022510002
022510509
022510541
022510525
Description
IsoTherm-System
includes IsoSafe, IsoRack, 0 °C IsoPack and -21 °C for
1.5/2.0 mL tubes
0.5 mL tubes
PCR-Cooler
Starter Set (1 x pink, 1 x blue)
Pink
Blue
27
MixMate® — Operating manual
EN
Index
Index
Abbreviations................................................ 6
Adjusting the volume ..................................20
Application fields........................................... 8
Application parameters............................... 25
Applications ..................................................8
Operating manual
Changing the parameters.
Mixing................................................... 17
Vortexing .............................................. 18
Cleaning ..................................................... 21
Continuous operation
Mixing................................................... 16
Vortexing .............................................. 18
Decontamination......................................... 23
Deepwell plate .............................................. 6
Dimensions................................................. 25
Disinfection ................................................. 23
Display........................................................ 12
LOCK.................................................... 18
M – 2 BACK.......................................... 20
M – 2 OFF ............................................ 20
M – 2 ON.............................................. 20
VOL ...................................................... 18
Disposal...................................................... 24
DWP .............................................................6
Environmental conditions............................ 25
Error messages ..........................................20
Front view ..................................................... 7
Glossary ....................................................... 6
Hazards ........................................................9
Incubate........................................................ 6
Insert
Plates in the plate holder ..................... 13
Plates in the tube holder ...................... 15
A
C
Tube holder in the plate holder ............ 14
Tubes in the tube holder ...................... 15
Insert plate ........................................... 13, 15
Inserting
Plates and tubes .................................. 13
Inserting tubes............................................ 15
Installation
Device.................................................. 11
Preparation .......................................... 11
Select location ..................................... 11
Intended use ................................................ 8
K
Key lock activation/deactivation.................. 20
L
D
LOCK.......................................................... 18
M
Mains power switch...................................... 7
Menu structure ........................................... 19
Menu/Enter................................................. 20
Micro test plate............................................. 6
Mixing......................................................... 15
Free parameters................................... 16
Preset parameters ............................... 15
Start ..................................................... 16
Mixing frequency
Maximum permissible .......................... 13
parameter............................................. 16
E
F
G
Mixing load ................................................... 6
Mixing time
parameter............................................. 16
MTP.............................................................. 6
O
OFF ............................................................ 20
Operator control elements.......................... 12
P
H
I
PCR plate
Semi-skirted........................................... 6
PCR-Platte
Skirted.................................................... 6
Unskirted................................................ 6
28
MixMate® — Operating manual
Pellet............................................................. 6
Performing a function test........................... 22
Plate holder................................................... 7
Power connection socket .............................. 7
Power supply .............................................. 25
Preset parameters ...................................... 16
Press start/stop
Mixing................................................... 16
Vortexing .............................................. 18
Product properties ........................................8
R
Rear view...................................................... 7
Resuspending............................................... 6
S
Safety Information......................................... 9
Select holder............................................... 13
Select location ............................................11
Semi-skirted.................................................. 6
Signal tone.................................................. 20
Skirted .......................................................... 6
Softkeys...................................................... 16
Software menu ........................................... 19
SPEED
Mixing frequency .................................. 16
Vortex frequency .................................. 18
Storage ....................................................... 24
T
Technical data
Application parameters......................... 25
Dimensions .......................................... 25
Environmental conditions ..................... 25
Power supply........................................ 25
Time
Mixing time ........................................... 16
Vortex time ........................................... 18
Transport .................................................... 24
Troubleshooting ..........................................20
tube formats.................................................. 8
Tube holder................................................... 7
Insert ....................................................14
Tubes
Select ...................................................13
U
Unskirted ...................................................... 6
V
VOL ............................................................ 18
Vortex frequency
set ........................................................ 18
Vortex mat.................................................... 7
Vortex time
set ........................................................ 18
Vortexing................................................ 6, 17
Free parameters................................... 18
Post-run facility .................................... 17
Start ..................................................... 18
Touch vortex mode............................... 17
W
Weight
Device.................................................. 25
Mixing load........................................... 13
Well .............................................................. 6
EN
Operating manual
29
MixMate® — Operating manual
EN
Operating manual
30
Declaration of Conformity
The device was tested in accordance with EN ISO 5349-1 : 2001 “Mechanical vibration – measurement
and assessment of human exposure to hand-transmitted vibration – Part 1”
Product name:
MixMate 5353
Product type:
Mixer for microtest tubes and plates with integrated touch vortex function
Hand-arm-vibration assessment of MixMate 5353 in accordance with DIN ISO 5349-1:2001
Tests were performed on the MixMate operated in the “touch vortex” operation mode (3500 rpm) with 15
ml and 50 ml Falcon tubes. The total vibration value was measured, the daily vibration exposure and the
exposure points calculated from it.
The exposure action value EAV of 2.5 m/s
2
A(8) is equivalent to 100 exposure points and is the level at
and above which hand-arm-vibration management procedures must be initiated where employees are
regularly exposed to this level of vibration exposure.
The exposure limit value ELV of 5 m/s
2
A(8) is equivalent to 400 exposure points and must not be
exposure times to limit daily vibration exposure to below the ELV.
Tube
Total
vibration
value
Daily
exposure
value
1 HOUR
e
Time – single tool
use:
For not exceeding
EAV ELV
m/s2 m/s2 2.5 m/s
2
minutes
5 m/s
2
minutes
Usage patter n : Each
test consisted of a
reprensent ative usage
pattern equiv a lent to 5
successive t ouch vortex
a total of 2 minutes 30
seconds “on” and 20
seconds “off”. Tubes
were filled wit h 2/ 3 of
water.
15 ml
50 ml
5.2
9.4
1.8
3.3
54
177
111
34
444
136
Exposure Action Value (EAV)
The table indicates that the operator using 15 ml Falcon tubes in touch vortex mode (3500 rpm) with
reported usage would reach the EAV in 111 minutes and using 50 ml Falcon tubes in
34 minutes. Based on a usage pattern of 30 seconds vortex / 5 seconds gap for changing
the tube, this means that approximately 190 of the 15 ml or 58 of the 50 ml Falcon tubes could produce
exposures that reach the EAV. With a usage pattern of 15 seconds vortex / 5 seconds gap, 333 x 15 ml
tubes or 102 x 50 ml tubes may be vortexed without exceeding the EAV.
With a usage pattern of 45 seconds vortex / 5 seconds gap 133 x 15 ml tubes or 40 x 50 ml tubes may be
vortexed.
exceeded on any work day. If it is, immediate measures must be taken to control vibration levels or reduce
operations of 30 seconds
with 5 second intervals in
xposure points
Exposure Limit Value (ELV)
The table indicates that the operator using 15 ml Falcon tubes in touch vortex mode (3500 rpm) with reported
usage would reach the ELV in 444 minutes and using 50 ml Falcon tubes in 136 minutes. Based on a usage
pattern of 30 seconds vortex / 5 seconds gap, this means that approximately 761 of the 15 ml or 233 of the 50
ml Falcon tubes could produce exposures that reach the ELV. Immediate action must thus be taken to limit
exposures to below the ELV. With a usage pattern of 15 seconds vortex / 5 seconds gap, 1332 x 15 ml tubes
or 408 x 50 ml tubes must be vortexed until the ELV is reached. With a usage pattern of 45 seconds vortex / 5
seconds gap 532 x 15 ml tubes or 163 x 50 ml tubes must be vortexed.
Number of tubes to reach
Vortex time
(5 sec gap)
EAV ELV
15 ml
EAV ELV
50 ml
15 sec
333
1332
102
408
30 sec
190
761
58
233
45 sec
133
532
40
163
Number of tubes for not exceeding
Vortex
time
EAV ELV
15 ml
EAV ELV
50 ml
15 sec
333
1332
102
408
30 sec
190
761
58
233
45 sec
133
532
40
163
(5 sec gap)
*
*) Sample measurement for this device only.
*
*) Sample measurement for this device only.
*
*) Sample measurement for this device only.
Your local distributor: www.eppendor f.com/worldwide
Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com
Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA
Tel: +1 516 334 7500 · Toll free phone: +1 800-645-3050 · Fax: +1 516 334 7506 · E-mail: info@eppendorf.com
Application Support Europe: Tel: +49 1803 666 789 (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min)
support@eppendorf.com
North America: Tel: +1 800 645 3050 · E-mail: techserv@eppendorf.com
Asia Pacific: Tel: +60 3 8023 6869 · E-mail: support_asiapacific@eppendorf.com
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