Eppendorf MixMate User Manual

5353 900.015

MixMate®

Operating manual

2007 Eppendorf AG, Hamburg. No part of this publication may be reproduced without

the prior permission of the copyright owner.

Trademarks

eppendorf

and MixMate® are registered trademarks of Eppendorf AG, Hamburg, Germany.

Trademarks are not marked in all cases with ™ or

in this manual.

5353 900.015-11/102011

MixMate® — Operating manual

Operating manual — MixMate®seeon p.Fig.Tab.p.

Operating manual

Table of contents

1 Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.1 Hazard icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3 Symbols used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.4 Abbreviations used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

1.5 Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

3.2 User profile. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

4.3 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

5.1 Overview of operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

5.2 Inserting plates and tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

5.2.1 Insert plate in the plate holder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

5.2.2 Insert the tube holder in the plate holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

5.2.3 Insert the plate in the PCR 96 tube holder . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.2.4 Insert tubes in the tube holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.3 Mixing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.3.1 Mixing with preset parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.3.2 Mixing with free preset parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5.4 Vortexing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.4.1 Touch vortex mode with 3500 rpm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.4.2 Using free parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.5 Device menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.5.1 Menu structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.5.2 Menu navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.5.3 Key lock (LOCK) activation/deactivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.5.4 Adjusting signal tone volume (VOL) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Operating manual

MixMate® — Operating manual

6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

7.2 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

7.3 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Operating manual

8.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

8.2 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

8.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

9.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

9.2 Ambient conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

9.3 Weight/dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

9.4 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

10 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

10.1 MixMate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

10.2 Tubes and plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

10.3 IsoTherm-System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

7.1.1 Cleaning the device and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

7.1.2 Performing a function test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

MixMate® — Operating manual

1 Operating instructions

1.1 Using this manual

 Please read this operating manual completely before using the device for the first time. Also

observe the instructions for use enclosed with the accessories.

 Please view this operating manual as part of the product and keep it somewhere easily

accessible.

 When passing the device on to third parties, be sure to include this operating manual.  If this manual is lost, please request another one. The current version can be found on our

website www.eppendorf.com

1.2 Danger symbols and danger levels

1.2.1 Hazard icons

Explosion Electric shock

Hazard point Material damage

1.2.2 Degrees of danger

The safety instructions in this operating manual indicate the following degrees of danger:

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

Operating manual

NOTICE May lead to material damage.

1.3 Symbols used

Depiction Meaning

 You are requested to perform an action.

Perform these actions in the sequence described.

• List.

Text Terms and key names from the software.

References useful information.

MixMate® — Operating manual

1.4 Abbreviations used

ANSI American National Standards Institute DNA Deoxyribonucleic acid DWP Deepwell plate MTP Microplate PCR Polymerase Chain Reaction RNA Ribonucleic acid rpm revolutions per minute SBS Society for Biomolecular Screening

Operating manual

1.5 Glossary

Deepwell plate Plate with 48, 96 or 384 wells with a larger volume than

Incubate Includes: cultivation of cell and bacterial cultures under

Micro test plate Plates with 24, 48, 96 or 384 wells for the preparation,

Mixing load All samples to be mixed and the tubes or plates in which

Pellet Compressed material. Is, for example, created via the

Resuspending Dissolve the pellet by vortexing in a liquid. The material

Semi-skirted PCR plate

Skirted PCR plate PCR plate with a surrounding edge.

microplates. Suitable for the preparation, mixing, centrifuging, transporting and storing of solid and liquid samples.

controlled ambient conditions.

mixing, centrifuging, transporting and storing of solid and liquid samples.

the samples are located.

centrifugation of a suspension.

is distributed in the liquid. The result is a suspension. PCR plate with surrounding half-edge.

Unskirted PCR plate PCR plate without a surrounding edge.

Vortexing Strong whirling or blending by manually pressing a tube

Well Cavity. Microplate, PCR plate or Deepwell plate tube.

onto the vortex mat.

MixMate® — Operating manual

2 Product description

2.1 Main illustration

Abb. 1:Front and rear view

Fig. 1: Front and rear view

1 Plate holder

For holding skirted PCR plates, MTP and DWP as well as tube holders.

3Softkeys

For selecting preset mixing parameters.

5 Operating controls

Keys for operating the MixMate (Fig. 3 on p. 12).

7 Power connection socket

Connection for the supplied power cable.

Operating manual

2Vortex mat

For direct vortexing of various tubes.

4Display

Displays the mixing frequency and the mixing time (Fig. 4 on p. 12).

6 Mains power switch

Switch for switching the device on and off. Switch position 0: the device is switched off. Switch position I: the device is switched on.

Abb. 2:Tube holder for the MixMate

Fig. 2: Tube holder for the MixMate

1 PCR 96 tube holder

For one PCR plate (96-well, semi-skirted or unskirted) or a max. of 96 tubes (0.2 mL).

3 1.5/2.0 mL tube holder

For max. 24 tubes (1.5 and 2.0 mL).

2 0.5 mL tube holder

For max. 24 tubes (0.5 mL).

MixMate® — Operating manual

2.2 Delivery package

Quantity Order No.

1 or

1 1

Operating manual

1 1- - Power cable

1 5353 900.015 1 5353 900.023 Short Instructions MixMate

2.3 Features

The MixMate allows aqueous solutions and suspensions to be mixed and vortexed effectively in a wide range of micro test tubes or plates. The MixMate supports from 0.2 mL PCR tubes to 2.0 mL micro test tubes as well as PCR, MTP and DWP plates up to 384 wells at a maximum mixing frequency of up to 3000 rpm.

The direct select buttons facilitate rapid access to selected mixing parameters (see p. 15). Potential applications include:

• controlled mixing of PCR, restriction or other enzyme reactions.

• controlled incubation of absorption, blocking or reaction preparations.

• resuspension of DNA, RNA, protein or cell pellets in tubes and in plates.

• vortexing in micro test tubes and in 15 mL/50 mL screw-top tubes.

(International)

5353 000.014 5353 000.022

5353 040.113 5353 040.121 5353 040.130

Order No. (North America)

022674226 022674200

022674005 022674021 022674048

Description

MixMate

230 V 120 V

Tube Holder

PCR 96

0.5 mL

1.5/2.0 mL

Operating Manual MixMate

multi-lingual

3 Safety

3Safety

3.1 Intended use

Exclusively intended for use indoors, the MixMate is used for mixing aqueous solutions and suspensions in closed tubes and closed plates.

Only use Eppendorf accessories or accessories recommended by Eppendorf.

3.2 User profile

This device may only be operated by trained and skilled personnel. Before using the device, read the operating manual carefully and familiarize yourself with the

device's mode of operation.

MixMate® — Operating manual

3.3 Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for material damage and personal injury is transferred to the operator:

• The device is not used in accordance with the operating manual.

• The device is used outside of its intended use.

• The device is used with accessories or consumables not recommended by Eppendorf.

• The device is maintained or repaired by persons not authorized by Eppendorf.

• The user makes unauthorized changes to the device.

3.4 Warnings for intended use

Read the operating instructions and observe the following general safety information before using the MixMate.

DANGER! Danger of explosion!

 Do not operate the device in areas where work is completed with explosive

DANGER!

substances.

 Do not use this device to process any explosive or highly reactive substances.  Do not use this device to process any substances which could create an explosive

atmosphere.

WARNING! Electric shock due to damage to device or mains cable.

 Only switch on the device if the device and mains cable are undamaged.

WARNING!

 Only use devices that have been properly installed or repaired.  In case of danger, disconnect the device from the mains supply.

WARNING! Lethal voltages inside the device.

 Ensure that the housing is always closed and undamaged so that no parts inside

WARNING!

the device can be contacted by accident.

 Do not remove the housing of the device.  Do not allow any liquids to penetrate the inside of the housing.  Do not allow the device to be opened by anyone except service personnel who have

been specifically authorized by Eppendorf.

Operating manual

WARNING! Device fire due to penetration of liquid

Penetration of liquid can cause a fire due to a short-circuit in the device.

WARNING!

 Do not allow any liquids to penetrate the inside of the housing.  Only mix in closed tubes and closed plates.  If liquid has penetrated the inside of the housing: switch off the device, pull the

power plug and have the device cleaned by service personnel authorized by Eppendorf.

MixMate® — Operating manual

WARNING!

Operating manual

WARNING!

WARNING! Injury from flying tubes and plates.

If the maximum permitted total weight of the mixing load is exceeded, plates or tubes may become detached from the device.

 Always ensure that tubes, plates and tube holder are well seated. The dimensions

of the plates used must comply with the ANSI/SBS standards for microplates

 Mix the DWP and the tube holders 0.5 mL, 1.5/2.0 mL and PCR 96 with max. 2000

rpm.

 Only mix mixing loads with a total weight of up to 80 g at maximum speed.  Only mix mixing loads with a total weight of 80 to 300g at speed of max. 2000 rpm.

WARNING! Injury from sample material being thrown out.

Sample material can be thrown out of open, improperly sealed or unstable tubes and plates.

 Only mix in closed tubes and closed plates.  Observe the nationally prescribed safety environment when working with

hazardous, toxic and pathogenic samples. Pay particular attention to the personal protective equipment (gloves, clothing, goggles etc.), extraction, and the safety class of the lab.

WARNING! Injury from improper vortex action.

Improper vortex action can destroy tubes or cause their content to be lost.

 Only vortex intact and sealed tubes.  Never vortex tubes made of glass or other fragile material.

WARNING! Risk from incorrect supply voltage

 Only connect the device to power supplies which correspond with the electrical

WARNING!

requirements on the nameplate.

 Only use sockets with a protective earth (PE) conductor and a suitable mains cable.

NOTICE! Damage to the display due to mechanical pressure.

 Do not exert mechanical pressure on the display.

NOTICE!

NOTICE! Caution! Strong vibration.

When mixing at high speeds, items located near the device may be moved by the

NOTICE!

vibrations of the work surface and, e.g., fall off the work table.

 Do not place easily movable items near the mixer or secure them adequately.

NOTICE! Damage to electronic components from spilled liquids.

 Make sure that the vortex mat and the cover caps are fitted properly. If the vortex

NOTICE!

mat is not fitted properly, contact your Eppendorf partner or the authorized Technical Service.

 If liquid has been spilt: Switch off the device, disconnect the power plug and

arrange for it to be cleaned by service personnel authorized by Eppendorf.

MixMate® — Operating manual

NOTICE! Damage to electronic components due to condensation.

After the device has been moved from a cool to a warmer environment, condensation

NOTICE!

may form inside the device.

 Wait at least three hours before connecting it to the power supply.

NOTICE! Damage from the use of aggressive chemicals.

 Do not use any aggressive chemicals on the device or its accessories, such as

NOTICE!

4 Installation

4.1 Preparing installation

Hint!

 Use the details on the scope of delivery (see Delivery package on p. 8) to check that delivery

is complete.

 Check all parts for any transport damage.

strong and weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.

 If the device becomes contaminated with aggressive chemicals, clean it

immediately with a mild cleaning agent.

Keep the transport carton and the packing material for subsequent safe transport or storage.

Operating manual

4.2 Selecting the location

Select a location for the MixMate in accordance with the following criteria:

• Mains power connection (230 V/120 V) as per device identification plate. This is located on

the rear side of the device.

• At least 10 cm away from adjacent devices and walls.

• Solid bench with stable, horizontal and even work surface.

4.3 Installing the instrument

1. Place theMixMate on a suitable work surface so that the air slots on the underside of the device are not blocked.

2. Connect the device to the mains power supply via the mains power socket 7 (see Fig. 1 on p. 7) using the mains cable supplied.

3. Switch on the device with the mains power switch 6 (see Fig. 1 on p. 7).

4. Carry out a test run at maximum speed (3000 rpm) to ensure that the grip between the device and the surface is sufficient. The MixMate must not move from its position.

MixMate® — Operating manual

5Operation

5 Operation

5.1 Overview of operating controls

Familiarize yourself with the operating controls and the display of the MixMate before using it for the first time.

Abb. 3:Operator control elements and displ ay

Operating manual

Fig. 3: Operator control elements and display

1 Soft key for MTP (384-well) 2 Soft key for PCR plates (384-well) 3 Soft key for MTP (96-well) 4 Soft key for PCR plates (96-well) and

5 Softkey for micro test tubes (1.5 and

2.0 mL)

7 Display 8 Start/stop mixing run 9 Set the mixing frequency (speed) 10 Set the mixing duration (time) 11 Navigate in the menu 12 Call up and select menu parameters

Abb. 4:Display

micro test tubes (0.2 and 0.5 mL)

6 Control LED to display the selected

softkey

Fig. 4: Display

1 Mixing time

Setting: up to 19:45 min. in 15 s increments, from 20 min. to 59 min. in 1 min. increments, from 1.0 h to 99.5 h in 0.5 h increments, 'oo': unlimited mixing time.

3 Mixing frequency

Setting: 300 to 3000 rpm in 50 rpm increments.

After switching on, the display shows the values of the last run.

Hint!

2 Symbol for key lock

4 Symbol for signal tone setting

+ 26 hidden pages