Please read this operating manual completely before using the device for the first time. Also
observe the instructions for use enclosed with the accessories.
Please view this operating manual as part of the product and keep it somewhere easily
accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our
website www.eppendorf.com
1.2 Danger symbols and danger levels
1.2.1Hazard icons
ExplosionElectric shock
Hazard pointMaterial damage
1.2.2Degrees of danger
The safety instructions in this operating manual indicate the following degrees of danger:
DANGERWill lead to severe injuries or death.
WARNINGMay lead to severe injuries or death.
CAUTIONMay lead to light to moderate injuries.
.
EN
Operating manual
NOTICEMay lead to material damage.
1.3 Symbols used
Depiction Meaning
You are requested to perform an action.
1.
2.
Perform these actions in the sequence described.
• List.
TextTerms and key names from the software.
References useful information.
5
MixMate® — Operating manual
EN
1.4 Abbreviations used
ANSIAmerican National Standards Institute
DNADeoxyribonucleic acid
DWPDeepwell plate
MTPMicroplate
PCRPolymerase Chain Reaction
RNARibonucleic acid
rpmrevolutions per minute
SBSSociety for Biomolecular Screening
Operating manual
1.5 Glossary
Deepwell plate Plate with 48, 96 or 384 wells with a larger volume than
Incubate Includes: cultivation of cell and bacterial cultures under
Micro test plate Plates with 24, 48, 96 or 384 wells for the preparation,
Mixing load All samples to be mixed and the tubes or plates in which
Pellet Compressed material. Is, for example, created via the
ResuspendingDissolve the pellet by vortexing in a liquid. The material
Semi-skirted PCR
plate
Skirted PCR plate PCR plate with a surrounding edge.
microplates. Suitable for the preparation, mixing,
centrifuging, transporting and storing of solid and liquid
samples.
controlled ambient conditions.
mixing, centrifuging, transporting and storing of solid
and liquid samples.
the samples are located.
centrifugation of a suspension.
is distributed in the liquid. The result is a suspension.
PCR plate with surrounding half-edge.
Unskirted PCR plate PCR plate without a surrounding edge.
Vortexing Strong whirling or blending by manually pressing a tube
Well Cavity. Microplate, PCR plate or Deepwell plate tube.
6
onto the vortex mat.
MixMate® — Operating manual
2 Product description
2Product description
2.1 Main illustration
Abb. 1:Front and rear view
Fig. 1: Front and rear view
1 Plate holder
For holding skirted PCR plates, MTP and
DWP as well as tube holders.
3Softkeys
For selecting preset mixing parameters.
5 Operating controls
Keys for operating the MixMate (Fig. 3 on
p. 12).
7 Power connection socket
Connection for the supplied power cable.
EN
Operating manual
2Vortex mat
For direct vortexing of various tubes.
4Display
Displays the mixing frequency and the
mixing time (Fig. 4 on p. 12).
6 Mains power switch
Switch for switching the device on and off.
Switch position 0: the device is switched
off. Switch position I: the device is switched
on.
Abb. 2:Tube holder for the MixMate
Fig. 2: Tube holder for the MixMate
1 PCR 96 tube holder
For one PCR plate (96-well, semi-skirted or
unskirted) or a max. of 96 tubes (0.2 mL).
The MixMate allows aqueous solutions and suspensions to be mixed and vortexed effectively in
a wide range of micro test tubes or plates. The MixMate supports from 0.2 mL PCR tubes to 2.0
mL micro test tubes as well as PCR, MTP and DWP plates up to 384 wells at a maximum mixing
frequency of up to 3000 rpm.
The direct select buttons facilitate rapid access to selected mixing parameters (see p. 15).
Potential applications include:
• controlled mixing of PCR, restriction or other enzyme reactions.
• controlled incubation of absorption, blocking or reaction preparations.
• resuspension of DNA, RNA, protein or cell pellets in tubes and in plates.
• vortexing in micro test tubes and in 15 mL/50 mL screw-top tubes.
(International)
5353 000.014
5353 000.022
5353 040.113
5353 040.121
5353 040.130
Order No.
(North
America)
022674226
022674200
022674005
022674021
022674048
Description
MixMate
230 V
120 V
Tube Holder
PCR 96
0.5 mL
1.5/2.0 mL
Operating Manual MixMate
multi-lingual
3 Safety
3Safety
3.1 Intended use
Exclusively intended for use indoors, the MixMate is used for mixing aqueous solutions and
suspensions in closed tubes and closed plates.
Only use Eppendorf accessories or accessories recommended by Eppendorf.
3.2 User profile
This device may only be operated by trained and skilled personnel.
Before using the device, read the operating manual carefully and familiarize yourself with the
device's mode of operation.
8
MixMate® — Operating manual
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised. Liability for
material damage and personal injury is transferred to the operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables not recommended by Eppendorf.
• The device is maintained or repaired by persons not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
3.4 Warnings for intended use
Read the operating instructions and observe the following general safety information before
using the MixMate.
DANGER! Danger of explosion!
Do not operate the device in areas where work is completed with explosive
DANGER!
substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which could create an explosive
atmosphere.
WARNING! Electric shock due to damage to device or mains cable.
Only switch on the device if the device and mains cable are undamaged.
WARNING!
Only use devices that have been properly installed or repaired.
In case of danger, disconnect the device from the mains supply.
WARNING! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside
WARNING!
the device can be contacted by accident.
Do not remove the housing of the device.
Do not allow any liquids to penetrate the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who have
been specifically authorized by Eppendorf.
EN
Operating manual
WARNING! Device fire due to penetration of liquid
Penetration of liquid can cause a fire due to a short-circuit in the device.
WARNING!
Do not allow any liquids to penetrate the inside of the housing.
Only mix in closed tubes and closed plates.
If liquid has penetrated the inside of the housing: switch off the device, pull the
power plug and have the device cleaned by service personnel authorized by
Eppendorf.
9
MixMate® — Operating manual
EN
WARNING!
Operating manual
WARNING!
WARNING!
WARNING! Injury from flying tubes and plates.
If the maximum permitted total weight of the mixing load is exceeded, plates or tubes
may become detached from the device.
Always ensure that tubes, plates and tube holder are well seated. The dimensions
of the plates used must comply with the ANSI/SBS standards for microplates
Mix the DWP and the tube holders 0.5 mL, 1.5/2.0 mL and PCR 96 with max. 2000
rpm.
Only mix mixing loads with a total weight of up to 80 g at maximum speed.
Only mix mixing loads with a total weight of 80 to 300g at speed of max. 2000 rpm.
WARNING! Injury from sample material being thrown out.
Sample material can be thrown out of open, improperly sealed or unstable tubes and
plates.
Only mix in closed tubes and closed plates.
Observe the nationally prescribed safety environment when working with
hazardous, toxic and pathogenic samples. Pay particular attention to the personal
protective equipment (gloves, clothing, goggles etc.), extraction, and the safety
class of the lab.
WARNING! Injury from improper vortex action.
Improper vortex action can destroy tubes or cause their content to be lost.
Only vortex intact and sealed tubes.
Never vortex tubes made of glass or other fragile material.
10
WARNING! Risk from incorrect supply voltage
Only connect the device to power supplies which correspond with the electrical
WARNING!
requirements on the nameplate.
Only use sockets with a protective earth (PE) conductor and a suitable mains cable.
NOTICE! Damage to the display due to mechanical pressure.
Do not exert mechanical pressure on the display.
NOTICE!
NOTICE! Caution! Strong vibration.
When mixing at high speeds, items located near the device may be moved by the
NOTICE!
vibrations of the work surface and, e.g., fall off the work table.
Do not place easily movable items near the mixer or secure them adequately.
NOTICE! Damage to electronic components from spilled liquids.
Make sure that the vortex mat and the cover caps are fitted properly. If the vortex
NOTICE!
mat is not fitted properly, contact your Eppendorf partner or the authorized
Technical Service.
If liquid has been spilt: Switch off the device, disconnect the power plug and
arrange for it to be cleaned by service personnel authorized by Eppendorf.
MixMate® — Operating manual
NOTICE! Damage to electronic components due to condensation.
After the device has been moved from a cool to a warmer environment, condensation
NOTICE!
may form inside the device.
Wait at least three hours before connecting it to the power supply.
NOTICE! Damage from the use of aggressive chemicals.
Do not use any aggressive chemicals on the device or its accessories, such as
NOTICE!
4 Installation
4Installation
4.1 Preparing installation
Hint!
Use the details on the scope of delivery (see Delivery package on p. 8) to check that delivery
is complete.
Check all parts for any transport damage.
strong and weak bases, strong acids, acetone, formaldehyde, halogenated
hydrocarbons or phenol.
If the device becomes contaminated with aggressive chemicals, clean it
immediately with a mild cleaning agent.
Keep the transport carton and the packing material for subsequent safe transport or
storage.
EN
Operating manual
4.2 Selecting the location
Select a location for the MixMate in accordance with the following criteria:
• Mains power connection (230 V/120 V) as per device identification plate. This is located on
the rear side of the device.
• At least 10 cm away from adjacent devices and walls.
• Solid bench with stable, horizontal and even work surface.
4.3 Installing the instrument
1. Place theMixMate on a suitable work surface so that the air slots on the underside of the
device are not blocked.
2. Connect the device to the mains power supply via the mains power socket 7 (see Fig. 1 on
p. 7) using the mains cable supplied.
3. Switch on the device with the mains power switch 6 (see Fig. 1 on p. 7).
4. Carry out a test run at maximum speed (3000 rpm) to ensure that the grip between the
device and the surface is sufficient. The MixMate must not move from its position.
11
MixMate® — Operating manual
EN
5Operation
5Operation
5.1 Overview of operating controls
Familiarize yourself with the operating controls and the display of the MixMate before using it for
the first time.
Abb. 3:Operator control elements and displ ay
Operating manual
Fig. 3: Operator control elements and display
1 Soft key for MTP (384-well)2 Soft key for PCR plates (384-well)
3 Soft key for MTP (96-well)4 Soft key for PCR plates (96-well) and
5 Softkey for micro test tubes (1.5 and
2.0 mL)
7 Display8 Start/stop mixing run
9 Set the mixing frequency (speed)10 Set the mixing duration (time)
11 Navigate in the menu12 Call up and select menu parameters
Abb. 4:Display
micro test tubes (0.2 and 0.5 mL)
6 Control LED to display the selected
softkey
Fig. 4: Display
1 Mixing time
Setting: up to 19:45 min. in 15 s
increments, from 20 min. to 59 min. in 1
min. increments, from 1.0 h to 99.5 h in 0.5
h increments, 'oo': unlimited mixing time.
3 Mixing frequency
Setting: 300 to 3000 rpm in 50 rpm
increments.
After switching on, the display shows the values of the last run.
Hint!
12
2 Symbol for key lock
4 Symbol for signal tone setting
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