Eppendorf Microplates User Manual

Deepwell Plates and Microplates

12 575.084-01/0809

Instructions for use

Copyright
the prior permission of the copyright owner.

eppendorf

©

2008 Eppendorf AG, Hamburg. No part of this publication may be reproduced without

®

and Eppendorf Plate® are registered trademarks of Eppendorf AG, Hamburg,

Germany.
Registered trademarks are not marked in all cases with ® in this manual.

0012 577.710-00/1209

Deepwell Plates and Microplates — Instructions for use

Instructions for use

1 Safety

 Please read this manual thoroughly before using the Eppendorf Deepwell Plates and

Microplates for the first time.

 Please view this manual as part of the product and keep it somewhere easily accessible.
 If this manual is lost, please request another one. The current version is available on our

website www.eppendorf.com, also in other languages.

WARNING! Damage to health from toxic, radioactive or aggressive chemicals as
well as infectious liquids and pathogenic germs.

WARNING!

 Observe the national regulations for handling these substances, the biological

 Wear personal protective equipment (PPE).

WARNING! Contamination risk from splashing liquids or escaping substances.

 Observe the maximum filling volume.

WARNING!

 Take appropriate safety measures to prevent the escape of substances in the plates

 Always remove the covers carefully.
 Only use undamaged and visibly perfect plates from intact packages.
 Do not use liquid nitrogen in combination with the plates. They could become

WARNING! Contamination risk from damaged plates during centrifugation.

The plates withstand heavy loads during centrifugation. However, they can be destroyed

WARNING!

and release the substances they contain if used improperly.

 Always observe the maximum permitted centrifugation forces.
 Read the operating manual of the centrifuge used.
 Centrifuge stacked plates at lower speed only.
 Note that organic solvents may reduce the mechanical load capability of the plates.

Instructions for use

Instructions for use

security level of your laboratory, the material safety data sheets and the
manufacturer's application notes.

during storage and transportation.

damaged or the covers could burst open during thawing.

If in doubt please contact the Eppendorf Application Support
([e-mail]).

CAUTION! Contamination risk when disposing of used consumables.

 Dispose of used consumables in accordance with the substances with which they

CAUTION!

have come into contact.

 Observe the rules applicable to your lab.

NOTICE! Material change at extreme temperatures.

The mechanical resistance and the dimensions of disposables are affected by extreme

NOTICE!

temperatures (e.g., refrigeration or autoclaving).

 Pay particular attention to this with automated applications.

3

Deepwell Plates and Microplates — Instructions for use

2 Technical data

Instructions for use

2.1 Eppendorf Deepwell Plate

Eppendorf Deepwell Plate 96/2000 μL 96/1000 μL 96/500 μL 384/200 μL

Theoretical total

[μL] 2400 1200 700 240

volume

Working volume [μL] 50 - 2000 30 - 1000 30 - 550 20 - 225

Position Well

[mm] a/b 11.2/14.4 9.0/12.1

center A1

Well/cavity

Distance of well

[mm] C 9.0 4.5

center

Depth [mm] 41 41 24 22

Length [mm] l 127.8

Width [mm] W 85.5

Height [mm] h 44.1 44.1 27.1 25.1

Plate

Base height [mm] bh 6.1

Stacking height

*

SH

*

The height of a plate stack is made up of a base plate of height h and other stacked plates each

[mm] 42.7 42.7 25.7 23.7

of stacking height SH.

4

Deepwell Plates and Microplates — Instructions for use

2.2 Eppendorf Microplate

Eppendorf Microplate 96/F-PP 96/U-PP 96/V-PP 384/F-PP 384/V-PP

Bottom shape flat round V-shape flat V-shape

Theoretical
total volume

[μL] 400 360 350 150 140

Working
volume

Position Well
center A1

Well/cavity

Distance of
well center

Depth [mm] 11.2 11.1 11.2 11.6 11.6

Length [mm] l 127.8

Width [mm] W 85.5

Height [mm] h 14.4

Plate

Base height [mm] bh 6.1

Stacking height

*

SH

*

The height of a plate stack is made up of a base plate of height h and other stacked plates each

of stacking height SH.

[μL] 50 - 350 20 - 320 20 - 300 10 - 120 5 - 120

[mm] a/b 11.2/14.4 9.0/12.1

[mm] C 9.0 4.5

[mm] 13.0

5

Deepwell Plates and Microplates — Instructions for use

Material Polypropylene, colored and colorless

Chemical resistance The plates, including border, show a high resistance to UV

Dimensions

Centrifugability
at 4 °C to 40 °C

2

Autoclaving Autoclavable (121°C, 20 min.). The dimensional stability of

Operational temperature -86°C to +100°C

Storage before use Store dry at room temperature. Protect from direct sunlight

Eppendorf Standard Quality Consumables in original Eppendorf Quality for routine

Eppendorf PCR clean
(lot certified)

Eppendorf Protein LoBind and
DNA LoBind

Eppendorf sterile (lot certified) Consumables are sterilized by irradiation. Best before: see

Current certificates can be found at www.eppendorf.com. Technical specifications subject to
change and errors excepted!

1

SBS: Society for Biomolecular Screening.

2

The centrifugation stability of any plate type is generally dependent on the plate itself,
centrifuge, rotor, rotor suspension gear, adapter, maximum rotation / maximum gravitation field,
operating temperature, centrifugation time, density of centrifuged liquid, organic solvent,
accumulated total weight and correct operation.

3

Centrifuge stacked plates at lower speed only.

light and chemicals. Compare Application No. 56: The best
material for original Eppis! on www.eppendorf.com

. In case

of doubt, contact Eppendorf Application Support.

1

In accordance with ANSI/SBS standard

I to IV, see

illustrations.

Eppendorf Deepwell Plates standard, DNA LoBind and
Protein LoBind quality: to 6000 x g.
Eppendorf Deepwell Plates sterile: to 5000 x g.
Eppendorf Microplates PCR clean, sterile, DNA LoBind and

Protein LoBind quality: to 6000 x g.

3

the disposables can be affected.

and UV light.

application.

Free of RNase, DNase, human DNA and PCR inhibitors.

Consumables with the highest recovery rates for proteins
and/or DNA/RNA molecules. They are PCR clean, i.e. free
from RNase, DNase, human DNA and PCR inhibitors.

label on folding box.

3 Product description

The Eppendorf Deepwell Plates and Microplates are uncoated polypropylene disposables for
analyzing, preparing, mixing, centrifuging, transporting and storing of solid and liquid probes.
Eppendorf Microplates can be used in all conventional plate readers. Only trained technical staff
may use these consumables.

The Eppendorf Deepwell Plates and Microplates are characterized by a high chemical and
temperature resistance and can be sealed using suitable sealing options. The consumables fulfill
the ANSI/SBS standards for microplates.

This product can be used in development, research, routine and training laboratories in industry,
in chemistry, and in training and instruction. The applications extend over the areas of life
science, biotechnology, chemistry and clinical research, but are not limited to these areas.

6

Instructions for use

Deepwell Plates and Microplates — Instructions for use

4 Ordering information

The Eppendorf Deepwell Plates and Microplates are also available in the following variants:

• Deepwell Plates: other border colors (yellow, red, green or blue).

• Large packs.

• Upon request with barcodes (see www.eppendorf.com/barcode).

You can find ordering information on suitable sealing options on
www.eppendorf.com/consumables

Further product information is available in our catalogue, on our website
www.eppendorf.com/consumables

4.1 Eppendorf Deepwell Plates

Instructions for use

.

and from Eppendorf Application Support.

Order No.
(International)

0030 501.306
0030 502.302
0030 504.305

0030 501.209
0030 502.205
0030 503.201
0030 504.208

0030 501.101
0030 502.108
0030 503.104
0030 504.100

0030 521.102
0030 522.109
0030 523.105
0030 524.101

Order No.
(North America)

951033405
951033502
0030504305

951032603
951032701
951032808
951032905

951031801
951031909
951032000
951032107

951031003
951031101
951031208
951031305

Description

Eppendorf Deepwell Plate 96/2000 μL

20 plates, wells clear, white border color
Standard
Sterile
Protein LoBind

Eppendorf Deepwell Plate 96/1000 μL

20 plates, wells clear, white border color
Standard
Sterile
DNA LoBind (also for RNA and other nucleic acids)
Protein LoBind

Eppendorf Deepwell Plate 96/500 μL

40 plates, wells clear, white border color
Standard
Sterile
DNA LoBind (also for RNA and other nucleic acids)
Protein LoBind

Eppendorf Deepwell Plate 384/200 μL

40 plates, wells clear, white border color
Standard
Sterile
DNA LoBind (also for RNA and other nucleic acids)
Protein LoBind

7

Deepwell Plates and Microplates — Instructions for use

4.2 Eppendorf Microplates

Order No.
(International)

0030 601.106
0030 602.102

0030 601.203
0030 602.200

0030 601.807 951040102

0030 601.572 951040145

0030 601.300
0030 602.307

0030 601.904 951040260

0030 601.670 951040308

0030 621.107
0030 622.103

0030 621.301
0030 622.308
0030 623.304
0030 624.300

0030 621.905 951040481

0030 621.670 951040503

Order No.
(North America)

951040005
951040021

951040048
951040081

951040188
951040227

951040341
951040383

951040421
951040464
951040546
951040589

Description

Microplate 96/F-PP

80 plates, wells clear, white border color
PCR clean
Sterile

Microplate 96/U-PP

80 plates, wells clear, white border color
PCR clean
Sterile

Microplate 96/U-PP

80 plates, wells black, white border color
PCR clean

Microplate 96/U-PP

80 plates, wells white, gray border color
PCR clean

Microplate 96/V-PP

80 plates, wells clear, white border color
PCR clean
Sterile

Microplate 96/V-PP

80 plates, wells black, white border color
PCR clean

Microplate 96/V-PP

80 plates, wells white, gray border color
PCR clean

Microplate 384/F-PP

80 plates, wells clear, white border color
PCR clean
Sterile

Microplate 384/V-PP

80 plates, wells clear, white border color
PCR clean
Sterile
DNA LoBind
Protein LoBind

Microplate 384/V-PP

80 plates, wells black, white border color
PCR clean

Microplate 384/V-PP

80 plates, wells white, gray border color
PCR clean

8

Eppendorf offices

Eppendorf offices

AUSTRALIA & NEW ZEALAND

Eppendorf South Pacific Pty. Ltd.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.com.au

CANADA

Eppendorf Canada Ltd.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorfna.com

FRANCE

Eppendorf France S.A.R.L.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.fr

ITALY

Eppendorf s.r.l.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.it

AUSTR IA

Eppendorf Austria GmbH
Phone: [phone] - 0
Fax: [phone] - 20
E-mail: [e-mail]
Internet: www.eppendorf.at

CHINA

Eppendorf China Ltd.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.cn

GERMANY

Eppendorf Vertrieb
Deutschland GmbH
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.de

JAPAN

Eppendorf Co. Ltd.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.jp

BRAZIL

Eppendorf do Brasil Ltda.
Phone: [phone]
Fax: [phone]
E-mail:
[e-mail]
Internet: www.eppendorf.com.br

CZECH REP. & SLOVAKIA

Eppendorf Czech & Slovakia s.r.o.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.cz /
www.eppendorf.sk

INDIA

Eppendorf India Limited
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.co.in

NORDIC

Eppendorf Nordic Aps
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.dk

SOUTH & SOUTHEAST ASIA

Eppendorf Asia Pacific Sdn. Bhd.
Phone: [phone]
Fax: [phone]
E-mail:
[e-mail]
Internet: www.eppendorf.com.my

UNITED KINGDOM

Eppendorf UK Limited
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.co.uk

SPAIN

Eppendorf Ibérica S.L.U.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.es

USA

Eppendorf North America, Inc.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorfna.com

SWITZERLAND

Vaudaux-Eppendorf AG
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.ch

OTHER COUNTRIES

Internet: www.eppendorf.com/
worldwide

9

Your local distributor: www.eppendorf.com/worldwide

Eppendorf AG · 22331 Hamburg · Germany · Tel: [phone] · Fax: [phone]

Eppendorf North America, Inc. · 102 Motor Parkway, Suite 410 · Hauppauge, NY 11788-5178 · USA

Tel: [phone] · Toll free phone: [phone] · Fax: [phone] · E-Mail: [e-mail]

Europe, International: Tel: [phone] · E-Mail: [e-mail]

North America: Tel: [phone] ext. 2258 · E-Mail: [e-mail]

Asia Pacific: Tel: [phone] · E-Mail: [e-mail]

E-Mail: [e-mail]

Application Support