Eppendorf Mastercycler pro User Manual

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Mastercycler pro

Operating manual Manual de instrucciones

Trademarks

2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior

Eppendorf registered trademarks of Eppendorf AG.

Registered trademarks are not marked with

Notice

The software of the Mastercycler pro contains open source software. License information is available as PDF documents via the Eppendorf node. To export the license documents, connect a memory device, select the Eppendorf node in the navigation tree and press the softkey.

Operating manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Manual de instrucciones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

, the eppendorf logo, Mastercycler®, SteadySlope®, vapoprotect® and Eppendorf twin.tec® are

or ™ in all cases in this manual.

6321 900.150-03/082013

Table of contents

Mastercycler pro

English (EN)

Operating manu al Mastercycler proseeon p.Fig.Tab.p.

English (EN)Operating manual

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.2.1 Mastercycler pro and Mastercycler pro S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.2.2 Mastercycler pro 384 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.3.1 Features of the Mastercycler pro variants. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2.3.2 Networking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.3 Note on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.5 Warning signs on the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4.1 Unpacking the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.2.1 Space requirements and bearing capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.2.2 Mains connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.3 Installing the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.4 Shut down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.1 Initial steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.1.1 Using the vapo.protect heated lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.1.2 Control panel keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.2 Initial start-up of the Mastercycler pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.2.1 Defining the administrator PIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.2.2 Other steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.3 User login and logout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.3.1 Logging in as a user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.3.2 Changing the user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.3.3 Logging out as a user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.4 Overview of operation with the control panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5.4.1 Navigation tree overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5.4.2 Using the navigation tree . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

5.4.3 Using external storage media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

5.4.4 Selecting export file formats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Table of contents

Mastercycler pro

English (EN)

5.5 Managing folders and programs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.5.1 Creating folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.5.2 Copying folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

5.5.3 Deleting folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

6 Programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

6.1 Overview of the program editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

6.1.1 Opening the program editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

6.1.2 Structure of the program editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

6.2 Defining general settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

6.2.1 Opening the header. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

6.2.2 Editing header settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

6.3 Editing programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

6.3.1 Inserting a program step. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

6.3.2 Editing parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

6.3.3 Deleting individual program steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

6.3.4 Deleting all program steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

6.3.5 Exporting a program listing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

6.3.6 Saving a program and exiting the editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

7 PCR run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

7.1 Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

7.1.1 Selecting the sample tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

7.1.2 Loading the thermoblock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

7.1.3 Sample volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

7.2 Starting the program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

7.2.1 Status view . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

7.3 Interrupting or stopping the program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

7.3.1 Interrupting the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

7.3.2 Continuing the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

7.3.3 Stopping the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

7.4 Using protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

7.5 Shutting down and switching off the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

8 System management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

8.1 Administrator functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

8.2 Managing user accounts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

8.2.1 Creating user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

8.2.2 Editing user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

8.2.3 Deleting user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

8.2.4 Changing the PIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

8.3 System settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

8.4 Cycler functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

8.4.1 Cycler system functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

8.4.2 Self test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

8.5 Managing and backing up data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

8.5.1 Backing up data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

8.5.2 Exporting and importing programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Table of contents

Mastercycler pro

English (EN)

8.6 Performing a control panel software update. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

8.6.1 Performing an automatic software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

8.6.2 Performing a manual software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

8.7 Transferring programs from the Mastercycler 533x. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

9 Quick start. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

9.1 Login . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

9.2 Creating folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

9.3 Copying folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

9.4 Deleting folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

9.5 Editing programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

9.6 Stopping and starting programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

9.7 Switching off the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

10.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

10.1.1 Cleaning the housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

10.1.2 Cleaning the heated lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

10.2 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

10.3 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

11.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

11.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

12 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

12.1 Packing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

12.2 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

12.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

13 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

13.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

13.2 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

13.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

13.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

13.5 Control panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

14 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

14.1 Mastercycler pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

14.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

14.3 Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

14.4 Recommended auxiliary materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

Table of contents

Mastercycler pro

English (EN)

Operating instructions

Mastercycler pro

English (EN)

1 Operating instructions

1.1 Using this manual

 Read the operating manual before you operate the device for the first time.

 Please view this manual as part of the product and keep it somewhere easily accessible.

 If this manual is lost, please request another one. The current version of the operating manual can be

found on our website at www.eppendorf.com

The sequences of actions in this operating manual are described for operation using the control panel and the language setting English.

Alternatively, you have the following possibilities:

• Operate the Mastercycler pro via a mouse (see p. 26).

• Change the dialog language (see p. 58).

1.2 Danger symbols and danger levels

Representation Meaning

DANGER

Risk of electric shock with potential for severe injury or death as a consequence.

DANGER

Risk of explosion with potential for severe injury or death as a consequence.

DANGER

Bio hazard with potential for risk to health or death as a consequence.

DANGER

Risk of burns.

WARNING

Warning of potential injury or health risk.

CAUTION

Refers to risk of damage to property.

Refers to particularly useful information and tips.

Operating instructions

Mastercycler pro

English (EN)

1.3 Symbols used

Depiction Meaning

 Actions without a specified order

• List

Text Display text or software text

1.4 Abbreviations used

ESP

Electronic Sample Protection

Actions in the specified order

Additional information

PCL

Printer Command Language (standard for controlling printers)

PCR

Polymerase Chain Reaction

PDF

Portable Document Format

Personal Identification Number

PostScript® (standard for controlling printers)

TSP

Thermal Sample Protection

USB

Universal Serial Bus

2 Product description

1 2 3 4

2.1 Main illustration

Abb. 2-1: Mastercyclerpro - front and rear view

Product description

Mastercycler pro

English (EN)

Fig. 2-1: Mastercycler pro - front and rear view

1Lid handle

For opening and closing and also locking and unlocking the heated lid.

2 vapo.protect heated lid

3 Address display

4 Status lamp

5 Rear plate with ventilation slots

6 Mains connection socket

7 Mains switch

0 = switched off, I = switched on.

8Terminal block

See detail drawing (Fig. 2-2 on p. 10).

9 Retaining clip

For control panel.

10 Thermoblock

11 Nameplate

Printer RS 232 Control Panel CAN_out CAN_in Term Address

1 2 3 4 5 6 7

1 2

3 4

Product description

Mastercycler pro English (EN)

Abb. 2-2: Terminal block

Fig. 2-2: Terminal block

1 Parallel interface

Connection for printer.

2 Serial interface (RS 232)

Connection for Eppendorf temperature validation system.

3 Control panel connection

4CAN out

Data outlet for connecting several Mastercycler pro or Mastercycler ep.

Abb. 2-3: Control panel

5CAN in

Data inlet for connecting several Mastercycler pro or Mastercycler ep.

6Term switch

Termination of CAN bus when networking several Mastercycler pro or Mastercycler ep. Lower position: ON. Upper position: OFF.

7 Address dial

Setting of device address when networking several Mastercycler pro or Mastercycler ep.

Fig. 2-3: Control panel

1Display

2USB port1

For mouse, printer or storage medium.

3USB port2

For mouse, printer or storage medium.

4 MMC slot

For data exchange via a MultiMediaCard (MMC).

Only devices meeting the IEC 950/EN 60950 (UL 1950) standards may be connected to the interfaces of the Mastercycler pro and the control panel.

2.2 Delivery package

The contents of the delivery package depend on the variant of your Mastercycler pro.

2.2.1 Mastercycler pro and Mastercycler pro S

Product description

Mastercycler pro

English (EN)

QuantityOrder no.

(International)

1 6321 000.019 - 230 V / 50 - 60 Hz or 6321 000.027 950030010 120 V / 50 Hz, with US- plug

1 6325 000.013 - 230 V / 50 - 60 Hz or 6325 000.021 950030020 120 V / 50 Hz, with US-plug

1 6321 900.150 -

1- -

1 - 100 pieces

1 - PCR clean, 2 pieces

1 - self-adhesive, 5 pieces

Order no. (North America)

Description

Mastercycler pro

Mastercycler pro S

Operating Manual Mastercycler pro

Certificate of Conformity

Mains cable

Eppendorf PCR tubes 0.2 mL

Eppendorf twin.tec skirted

96-well PCR plates

PCR film

2.2.2 Mastercycler pro 384

QuantityOrder no.

(International)

1 6324 000.010 - 230 V / 50 - 60 Hz or 6324 000.028 950030030 120 V / 50 Hz, with US-plug

1- -

1- 2 pieces

1 - self-adhesive, 5 pieces

Order no. (North America)

Description

Mastercycler pro 384

Operating manual

Certificate of Conformity

Mains cable

Eppendorf twin.tec

384-well PCR plates

PCR film

Product description

Mastercycler pro English (EN)

2.3 Features

Product variants

The Mastercycler pro is available in three variants.

• Mastercycler pro

• Mastercycler pro S

• Mastercycler pro 384

All variants are provided with the following features:

Triple Circuit Technology

All Mastercycler pro thermomodules feature extremely fast heating and cooling rates. Triple Circuit Technology ensures a homogeneous temperature distribution and enables the generation of specific temperature gradients for PCR optimizations.

SteadySlope

SteadySlope Technology ensures that the heating and cooling ramp rates for thermal modules are identical in both gradient and normal operation. That means you can expect identical temperature control characteristics in both optimization and routine experiments, as well as a reliable transfer of the optimization results to the routine application.

vapo.protect

All variants of the Mastercycler pro have avapo.protect heated lid. A flexible pressure element ensures that force is applied evenly to the surface of the tubes or plate. This reduces evaporation from the reaction sample effectively. The vapo.protect heated lid performs automatic tube height adjustment for all sample tubes and PCR plates.

TSP

The heated lid of the Mastercycler pro series is equipped with TSP technology (Thermal Sample Protection). The thermoblock is continuously kept at 20°C during the lid heating phase. This means TSP reduces the thermal load on your samples and minimizes the probability of non-specific product formation during the PCR.

Self-test function

You can use the integrated self test function of the Mastercycler pro in combination with the control panel with USB ports to check whether the thermoblock is functioning properly without the need for any further tools. A separately available USB key is required for the self test function (see Accessories on p. 85).

Control

The Mastercycler pro is controlled via the control panel. It can also be controlled via the PC software CycleManager pro.

Product description

Mastercycler pro

2.3.1 Features of the Mastercycler pro variants

Variant Features

Mastercycler pro • Freely programmable temperature gradient over 12 rows.

• A gradient range of up to 20°C.

•Robust, specially coated aluminum block.

• Holds up to 96 PCR tubes (0.2 ml) or a 96-well PCR plate.

Mastercycler pro S • Highest temperature control speed due to silver block: up to 6°C/s.

• Standard PCR possible in under 30 min.

• Pulse function for device-driven hot start for avoiding non-specific PCR product formation.

• Freely programmable temperature gradient over 12 rows.

• Extended gradient range of up to 24°C.

• Holds up to 96 PCR tubes (0.2 ml) or a 96-well PCR plate.

Mastercycler pro 384 • Freely programmable temperature gradient over 24 rows.

• A gradient range of up to 20°C.

• Holds a 384-well PCR plate.

English (EN)

2.3.2 Networking

Mini-satellite system

Up to five devices from the Mastercycler pro or Mastercycler ep series can be interconnected and operated via a central control panel. The procedure for setting up this type of configuration is described separately (see Operating multiple Mastercycler pro via a control panel ("mini-satellite" system) on p. 23).

PC control

You can use the PC software CycleManager pro to control up to 30 devices from the Mastercycler pro or Mastercycler ep series in a network. This software is available separately for purchase (see Accessories on p. 85).

Product description

Mastercycler pro English (EN)

Safety

Mastercycler pro

English (EN)

3Safety

3.1 Intended use

The Mastercycler pro is intended to be used in research and routine laboratories in the field of biomedical sciences.

The Mastercycler pro is used to control the temperature of aqueous solutions, suspensions, and emulsions in closed micro test tubes for enzymatic reactions, typically for polymerase chain reaction (PCR).

The Mastercycler pro is intended exclusively for indoor use.

3.2 User profile

This device must only be used by skilled personnel with the appropriate training.

Before using the device, read the operating manual carefully and familiarize yourself with the device's mode of operation.

3.3 Note on product liability

In the following cases the protection provided in the device may be impaired. The liability for the function of the device passes to the operator if:

• The device is not used in accordance with the operating manual.

• The device is used outside the range of application described herein.

• The device is used with accessories or consumables (e.g. tubes and plates) which are not recommended by Eppendorf.

• The device is maintained or repaired by persons not authorized by Eppendorf.

• The owner has made unauthorized modifications to the device.

3.4 Warnings for intended use

Read the operating manual first and follow the general safety instructions below before using the Mastercycler pro.

DANGER! Risk of explosion!

 Do not operate the device in rooms where work is being carried out with explosive

substances.

 Do not use this device to process any explosive or highly reactive substances.  Do not use this device to process any substances which could create an explosive

atmosphere.

Safety

Mastercycler pro English (EN)

DANGER! Risk when handling toxic or radioactively-marked liquids or pathogenic germs.

 Follow national regulations governing the handling of these substances.  For complete instructions regarding the handling of germs or biological material of risk

group II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health Organization, current edition of the Laboratory Biosafety Manual).

WARNING! Risk to health and bio hazard when operated with lid open.

The tube lids may pop open at high block temperatures.

 Close the lid of the device when heating samples.

DANGER! Electric shock as a result of penetration of liquid.

 Switch off the device and disconnect it from the power supply before starting cleaning or

disinfecting.

 Do not allow any liquids to enter the inside of the housing.  Do not perform spay disinfection.  Only reconnect the device to the power supply once it is completely dry.

DANGER! Lethal voltages inside the device.

 Ensure that the housing is always closed and undamaged so that no parts inside the device

can be contacted by accident.

 Do not remove the housing of the device.  Do not allow the device to be opened by anyone except service personnel who have been

specifically authorized by Eppendorf.

DANGER! Electric shock.

 Switch off the device and disconnect the power plug before starting to maintain or clean.

WARNING! Damage due to incorrect power supply.

 Only connect the device to power sources that match the electrical specifications on the

device's nameplate.

 Use only sockets with protective earth.

WARNING! Injury from trapping or crushing.

 Do not place your fingers between the lid and the housing when opening and closing the

device or when opening it into the cleaning position.

 Do not reach in under the open lid.  Take care that the stand is properly engaged so that the lid stays in the opened position.

Safety

Mastercycler pro

English (EN)

WARNING! Burns from hot thermoblock and heated lid.

The thermoblock, the inner side of the heated lid and micro test tubes/PCR plates can reach temperatures of over 50 °C very quickly.

 The heated lid should be kept closed until temperatures of 30 °C or less are reached.  Take note of the symbols on the thermoblock warning that surfaces may be hot.  If you are using well caps consisting of thermally insulating material, please keep in mind

that the temperature on the inside of the heated lid can be significantly higher than the temperature displayed.

 Before starting cleaning wait for the thermoblock and heated lid to cool down.

WARNING! Injury from sharp-edged components.

When the Mastercycler pro is in the open position, sharp-edged components are exposed. When cleaning the Mastercycler pro, the sharp edges on the rollflex cable and the guide rail may cause injuries.

 Take special care and wear protective gloves.

WARNING! Risk to health if used incorrectly.

If incubation is performed incorrectly, sample material may be released and the thermoblock and the heated lid damaged.

 Ensure that the PCR plates are correctly inserted in the thermoblock.  Always close the heated lid before heating the samples to prevent the lids of the tubes from

bursting open.

WARNING! Risk to health from contaminated device

 Perform decontamination before storing or dispatching the device and/or its accessories.

CAUTION! Unsuitable tubes can result in sample material escaping.

Unsuitable tubes may be so severely damaged that sample material is released.

 Use only tubes whose properties meet at least the requirements specified in these

operating instructions.

 This is especially important when working with infectious material.

WARNING! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, function and precision of the device. Eppendorf accepts no warranty or liability for damage caused by non-recommended accessories and spare parts or incorrect use.

 Use only accessories recommended by Eppendorf and original spare parts.

Safety

Mastercycler pro English (EN)

WARNING! Risk of injury when lifting and transporting the device.

Lifting the device with the wrong posture may cause serious damage to your health. If the device falls to the ground it can cause injuries.

 Do not lift the device by the handle or the housing of the heated lid.  Carry the device securely with both hands to prevent it from slipping.  Always keep a straight back when lifting and carrying the device.

CAUTION! Damage to heated lid from sharp-edged sample tubes or objects.

 Use only tubes whose properties meet at least the requirements specified in these

operating instructions.

 Do not use any sharp-edged or sharp objects on the heated lid.  Extra caution should be exercised when cleaning so as not to damage the heated lid.

CAUTION! Escaping liquid can cause damage to the device.

If the flexible pressure unit of the heated lid is damaged, liquid can escape and enter the device.

1. Leave the lid open.

2. Disconnect the power plug immediately from the power supply.

3. Contact Eppendorf Service.

CAUTION! Damage to the thermoblock from incorrect operation.

 Do not fill sample material directly into the thermoblock.

CAUTION! Damage to electronic components through formation of condensation.

After the device has been moved from a cool to a warmer environment, formation of condensation can occur inside the device.

 Allow the device to warm up for at least 12 hours after being set up before you connect it

to the mains power supply.

CAUTION! Material damage from uneven work surface.

 Position the device on level and stable work surface which is capable of bearing the weight

of the device. The device must not be placed on a trolley or at an angle.

CAUTION! Damage due to overheating.

 Do not place the device close to sources of heat (e.g. radiator, drying cabinet).  Do not expose the device to direct sunlight.  Allow air to circulate freely by leaving at least 10 cm to adjoining devices or to the wall and

keep the underside of the device clear.

CAUTION! Lack of safety due to missing operating manual.

 When passing on the device, always enclose the operating manual.  If you lose the operating manual, request a replacement. The current version of the

operating manual and the safety instructions can also be found on our websitewww.eppendorf.com

3.5 Warning signs on the device

This section explains the warning signs used on the Mastercycler pro.

Safety

Mastercycler pro

English (EN)

1 WARNING

Risk of burns from the hot thermoblock (triangle with tip facing down) or the hot inner side of the heated lid (triangle with tip facing up). Gradient operation can result in the temperature being hotter on the right side than on the left side

2 WARNING

Risk of burns from hot surfaces.

Safety

Mastercycler pro English (EN)

Installation

Mastercycler pro

English (EN)

4 Installation

4.1 Unpacking the device

WARNING! Risk of injury when lifting and transporting the device.

Lifting the device with the wrong posture may cause serious damage to your health. If the device falls to the ground it can cause injuries.

1. Release the four plastic locking clips at the long sides of the carton by pulling out the flaps.

2. Lift the carton lid and take the foam upper part off the Mastercycler pro.

3. Take the carton insert out of the carton lid (contains the mains cable).

4. Take the Mastercycler pro out of the plastic bag.

5. With both hands, hold the Mastercycler pro by the lower half of the housing and lift it out of the package.

6. Remove all the packing material.

7. Visually check the device to ensure that no damage has occurred during shipment.

8. Check the completeness of the delivery (see Delivery package on p. 11).

Never use a device if it shows any signs of damage. In this case, contact your distributor or the appropriate Eppendorf office.

• Retain the packing material for shipping or storing the Mastercycler pro at a later date.

• Ship the Mastercycler pro only in the original packing material.

Installation

Mastercycler pro English (EN)

4.2 Selecting the location

4.2.1 Space requirements and bearing capacity

CAUTION! Material damage from uneven work surface.

 Position the device on level and stable work surface which is capable of bearing the weight

of the device. The device must not be placed on a trolley or at an angle.

CAUTION! Damage due to overheating.

keep the underside of the device clear.

Information regarding the dimensions and weight of the device components can be found separately(see Weight/dimensions on p. 83).

When selecting a location for the Mastercycler pro ensure that the following criteria are met:

• The surface must have sufficient bearing capacity and ensure the required stability.

• The feet at the front of the unit must be secured to prevent them from slipping.

• The device must be positioned at a height which provides for convenient and safe operation.

• Additional space is required for free air circulation at the back of the device (10 cm) and for the control panel at the front (14 cm).

4.2.2 Mains connection

CAUTION! Connected devices may be shut down due to a power supply overload.

The total current consumption of the devices can exceed the maximum permitted supply rating when multiple power sockets are used. In this case, the mains fuse interrupts the electric circuit. The connected devices are shut down.

 Do not use multiple sockets when connecting several Mastercycler pro.

Information on the power consumption of the Mastercycler pro is provided separately (see Power supply on p. 83).

• You need an earthed wall socket for the power supply of the Mastercycler pro. Use the supplied power cable to connect the Mastercycler pro.

• The control panel is supplied via the Mastercycler pro and does not require an additional power supply.

Installation

Term

Mastercycler pro

English (EN)

4.3 Installing the instrument

This section describes how to start up the Mastercycler pro with the control panel and how to operate several devices belonging to the Mastercycler pro and Mastercycler ep series with each other in a network.

The connections and switches are located at the back of the device (see Main illustration on p. 9).

• Follow the steps below to define the settings and connect the devices before switching on

the Mastercycler pro.

• Never disconnect the control panel from a Mastercycler pro that is switched on.

4.3.1 Operating a Mastercycler pro via a control panel

1. Connect the cable on the control panel to the Control Panel connector of the Mastercycler pro.

2. Set Term switch to ON (lower position).

3. Use the address dials to set the address of the Mastercycler pro to 01. To do this, turn the left dial into position 0 and the right dial to 1, as shown in the illustration.

4. Connect the power cable to the Mastercycler pro and to the mains supply.

4.3.2 Operating multiple Mastercycler pro via a control panel ("mini-satellite" system)

CAUTION! Connected devices may be shut down due to a power supply overload.

 Do not use multiple sockets when connecting several Mastercycler pro.

A control panel can be used to control up to five devices belonging to the Mastercycler pro or Mastercycler ep series (also in combination). Each additional device connected requires its own CAN bus cable. CAN bus cables are available separately for purchase (see Accessories on p. 85).

CAN_out

CAN_in

Term

CAN_out Control PanelCAN_in Term Address CAN_out CAN_in Term AddressCAN_out CAN_in Term Address

Installation

Mastercycler pro English (EN)

1. Connect the cable on the control panel to the "Control Panel" connector of the first device.

2. Connect the CAN bus cable to the "CAN_out" connector of the first device.

3. Connect the free end of the CAN bus cable to the "CAN_in" connector of the second device.

Connecting additional devices

4. Always use the CAN bus cable to connect the "CAN_out" connector of the last connected device to the "CAN_in" connector of the new device.

Device 3 Device 2 Device 1

Abb. 4-1: Address and Term settings and wiring connections for a network consisting of t hree devices

5. Set the Term switch of the first and last device to "ON" (lower position).

6. Set the Term switch of all other devices to "OFF" (upper position). Note: If the network consists of exactly two devices, the Term switch of both devices must be set to "ON".

7. For the first device connected to the control panel, set the address to 01.

8. Define one unique address from 02 to 05 on each additional device.

Fig. 4-1: Address and Term settings and wiring connections for a network consisting of three devices

Each address may only be assigned once within the network. The address that was set for the Mastercycler pro will be displayed by the two-digit address display 3 on the heated lid during operation (see Main illustration on p. 9).

Installation

Mastercycler pro

English (EN)

4.3.3 Connecting Mastercycler pro to an existing Mastercycler ep

You can extend an existing system of one or more Mastercycler ep devices with a corresponding control panel using devices of the Mastercycler pro series.

When operating the Mastercycler pro with a Mastercycler ep control panel (without USB connections), the following restrictions apply:

• The self test function is not available.

• The PDF file format is not available for exporting programs, protocols and log files.

• No USB devices can be connected. Information for connecting a PS/2 mouse can be found in the Mastercycler ep operating manual.

Prerequisites

Before a Mastercycler pro can be connected to an existing Mastercycler ep system, the software of the control panel and of the connected Mastercycler ep has to be updated. For more information, contact the Eppendorf branch for your country (see back cover flap).

1. Follow the instructions in the Mastercycler ep operating manual to update the control panel software.

2. After restarting the control panel, synchronize all connected Mastercycler ep with the control panel.

3. Shut down the control panel (see p. 54) and switch off all Mastercycler ep devices.

4. Set the address of the new Mastercycler pro to the next free address number.

Example:

If you already operate two Mastercycler ep devices, set the address of the new Mastercycler pro to 03.

5. Connect the cables and define the Term settings.

6. Switch all devices on and register them at the control panel.

The Mastercycler pro is displayed as an additional cycler node on the control panel.

4.3.4 Using the USB ports

The control panel of the Mastercycler pro has two USB ports (see Fig. 2-3 on p. 10) for connecting a mouse, printer, storage medium or the self test USB key.

 If you would like to use more than two USB devices at the control panel at the same time, you can make

use of a USB hub.

Installation

Mastercycler pro English (EN)

4.3.5 Connecting the printer

The Mastercycler pro can be connected to a parallel interface printer in order to print protocols and programs in text format.

 Connect the printer via a parallel data cable to the parallel interface (see Fig. 2-2 on p. 10) of the

Mastercycler pro.

A printer with USB interface can be connected to the control panel to print protocols, programs and self test certificates.

Prerequisites

The USB printer must be compatible with the PostScript can be found in the operating manual for your printer.

1. Us a USB cable to connect the printer to one of the two USB ports (see Fig. 2-3 on p. 10) of the control panel.

or PCL printer standards. Further information

4.3.6 Connecting the mouse

You can connect a USB mouse (not included in the delivery package) to the control panel.

 Connect the plug of the mouse to one of the USB ports on the right-hand side of the control panel (see

Fig. 2-3 on p. 10).

The mouse pointer appears in the display.

4.4 Shut down

Proceed as follows if you do not want to use the Mastercycler pro for longer than a week.

1. Load the block with an empty PCR plate(see p. 50).

2. Close the heated lid and flip the handle forwards (see p. 27).

3. Disconnect the Mastercycler pro from the power supply.

Do not leave the lid of the Mastercycler pro in an open position in order to avoid the block from becoming contaminated.

Operation

Mastercycler pro

English (EN)

5 Operation

5.1 Initial steps

Check the correct installation

Before the Mastercycler pro is started up for the first time, ensure the following:

• The device is correctly connected.

• The device is free of damage.

• Make sure that air can circulate freely around the ventilation slots on the rear of the device and around the fan behind the feet.

5.1.1 Using the vapo.protect heated lid

Thevapo.protectheated lid features a heated flexible pressure unit. When the lid closes, the pressure unit comes to rest lightly on the surface of the sample tubes. A liquid cushion in the pressure unit is then used to create a pressure to ensure that the tubes are pressed uniformly into the thermoblock thereby protecting the tube seals against evaporation. The heating process prevents condensation from forming in the upper area of the tube when the reaction liquid is heated.

5.1.1.1 Closing the heated lid

Prerequisites

The thermoblock of the Mastercycler pro is loaded with sample tubes or a PCR plate (see Loading the thermoblock on p. 50).

CAUTION! Damage to heated lid from sharp-edged sample tubes or objects.

 Use only tubes whose properties meet at least the requirements specified in these

operating instructions.

 Do not use any sharp-edged or sharp objects on the heated lid.  Extra caution should be exercised when cleaning so as not to damage the heated lid.

Operation

Mastercycler pro English (EN)

1. Pull vapo.protect heated lid forwards at the lid handle as far as possible so that the lid handle can be rotated.

2. Flip the handle over the heated lid until it clicks into place.

You can now start a program run or manually temperature-control the lid and the samples.

5.1.1.2 Opening the heated lid

WARNING! Risk to health and bio hazard when operated with lid open.

The tube lids may pop open at high block temperatures.

 Close the lid of the device when heating samples.

WARNING! Risk of burns from the hot thermoblock and heated lid.

The thermoblock and the heated lid very quickly reach temperatures above 95 °C.

 Keep heated lid closed until the temperature of the thermoblock is below 30 °C.

Open the heated lid in one go as follows:

 Flip the lid handle up over the heated lid and push the heated lid by the handle to the rear position until

it stops.

Operation

Mastercycler pro

English (EN)

5.1.1.3 Status lamp

The vapo.protect heated lid is equipped with a status lamp 2(see Main illustration on p. 9) which allows the current operating status of the Mastercycler pro to be visible from far off.

Status lamp Operating status

Illuminates green The Mastercycler pro is idle.

Flashes green A program run is active.

Flashes orange The program run is waiting for user intervention in a pause or hold step(see

Editing programs on p. 44).

Flashes red A fault has occurred. Additional information on the type of fault is displayed on

the control panel (see Error messages on p. 77).

5.1.1.4 Address display

The vapo.protect heated lid is equipped with a two-digit address display so that the cycler can be identified easily (see Main illustration on p. 9).

The displayed address number matches the cycler numbering in the operating software. The address setting is described separately.

Delete

Enter

Stop

Start

abc

jkl mno

tuv wxyz

def

pqrs

ghi

Start

Stop

DeleteDelete

EnterEnter

Delete

abc

jkl mno

tuv wxyz

def

pqrs

ghi

Operation

Mastercycler pro English (EN)

5.1.2 Control panel keys

Start the selected program.

Stop the running program.

Function keys The function of the key changes with the software dialog and appears in the display immediately above the key.

• Step-by-step navigation. The current position of the cursor is marked.

• Move the cursor within an alphanumerical input field.

Move cursor into the next input field.

Delete the following elements:

• Delete characters that are to the right of the cursor.

• Marked signs.

• Marked folders or programs.

• Confirm input.

• Open selection lists.

• Enter digits and text in input fields.

In the case of numerical input fields (e.g. temperature and time entries) the keys only represent numbers. For decimal numbers with numbers after the decimal point, use the period as a decimal point.

Alphanumerical input fields have keys with multiple functions.

 To enter text, keep pressing the key with the relevant letter until the desired letter

is displayed.

Example: To e nter pcr 3 in a text field, press the following keys in quick succession:

1. for p: press 7 once

2. for c: press 2 three times

3. for r: press 7 three times

4. for the blank space: press 0 (zero) twice

5. for 3: press 3 four times

Operation

Mastercycler pro

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5.2 Initial start-up of the Mastercycler pro

During the initial startup of the control panel you can define an administrator PIN in order to protect the system against access by unauthorized persons. These data are saved in the control panel and are not lost when the Mastercycler pro is switched off.

5.2.1 Defining the administrator PIN

If no administrator PIN has been defined, you are prompted to do so.

CAUTION! Loss of data due to misuse or loss of administrator PIN.

The administrator PIN protects the system from undesired access to its configuration and to the stored data of all users.

 Make a note of the administrator PIN and keep it in a safe place. If you lose the

administrator PIN, contact Eppendorf Service.

 Allow access to the administrator PIN only to persons who are allowed to edit the

configuration of the system and who have the knowledge required to do this.

If you do not want to use the user management option, you do not have to define an administrator PIN. In this case, leave the input fields of the Input Admin PIN window empty and deactivate the PIN function. Further information on user management is provided separately (see p. 58).

Delete

abc

jkl mno

tuv wxyz

def

pqrs

ghi

DeleteDelete

Operation

Mastercycler pro English (EN)

To define the administrator PIN, perform the following steps in the sequence described.

1. Switch on the Mastercycler pro at the mains power switch. The Input Admin PIN window appears.

2. Enter the desired administrator PIN using the numerical keys on the control panel. The PIN can be between 4 and 8 digits long.

3. Go to the Confirmation field and enter the PIN again to confirm.

4. If your two PIN entries do not match each other, an error message is displayed. In this case, delete the incorrectly entered PIN and repeat the PIN entries.

5. Confirm by pressing the OK function key.

After successful confirmation of the administrator PIN, the navigation tree appears in the control panel display. You are now logged in as the administrator.

•If the PIN function is activated, you must define an administrator PIN. Otherwise this input prompt will appear each time the device is switched on.

• You can also change the administrator PIN subsequently (see Changing the PIN on p. 56).

5.2.2 Other steps

5.2.2.1 Setting the date and time

The date and time of the control panel are displayed in the upper right-hand corner of the display. As the administrator you can change this internal clock at any time (see p. 58).

5.2.2.2 Setting up user accounts

To be able to use the Mastercycler pro, you must set up at least one user account. Detailed information about setting up user accounts and about user administration can be found separately(see Managing user accounts on p. 55).

Operation

Enter

NextNext

Delete

abc

jkl mno

tuv wxyz

def

pqrs

ghi

Mastercycler pro

English (EN)

5.3 User login and logout

The functions described here are only available if the PIN function is activated.

5.3.1 Logging in as a user

Before you can log in under your user name, your administrator must have set up a user account for you.

Perform the following steps in the sequence described.

1. Switch on the Mastercycler pro at the mains power switch. The control panel switches on automatically and the software is loaded. When the software has been loaded, the control panel displays the User

Loginwindow.

2. Open the list with the Enter key and select the user name.

3. Press theNext key. The cursor switches to the PIN field.

4. Enter your personal PIN using the numerical keys on the control panel. If the PIN is wrong, the message Wrong PIN appears. Use theLogin function key to return to the User Login window, delete the incorrectly entered PIN with the Delete key and repeat the PIN entry.

5. Press the OK function key to confirm the entry. The navigation tree is displayed on the control panel.

You are now logged in under your user name and can work with the Mastercycler pro.

5.3.2 Changing the user

If another user is already logged in, you can log in as follows:

Operation

Mastercycler pro English (EN)

1. Mark your user node.

2. Press the Login function key.

3. Log in as a user.

The user who was previously logged on is automatically logged out.

5.3.3 Logging out as a user

You can log yourself out from the control panel to protect your programs against unauthorized changes.

1. Mark your user node.

2. Press the Logout function key.

You are now logged out. The guest user is automatically logged in. Guest users cannot start any programs and just have read-only rights for all programs and folders in the control panel.

5.4 Overview of operation with the control panel

5 6

5.4.1 Navigation tree overview

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1 Window title

Displays the current level (user level, cycler level, top level) in the navigation tree and the title of dialog windows. A status symbol is displayed on the right-hand side of the window title if the external storage medium (MMC or USB) is accessed.

2 Main node

Eppendorf is the main node containing all the

other nodes.

3User node

The main node contains a user node for each user who has been set up. You can store folders and programs below your own user node. You can start the programs in your own user node on every cycler that is connected.

4Cycler node

Each cycler that is connected and switched on is displayed by a separate cycler node. Here you can open the status view of the respective cycler and access additional cycler-specific functions. The numbering corresponds to the address of the cycler.

5System node

This node contains the most important system settings. Some settings can only be edited by the administrator.

6 MMC and USB nodes

These two nodes are only visible if an appropriate storage medium is inserted into the control panel. These nodes can be used to access external storage media (MMC or USB), e.g. for transferring programs to a different control panel or for a system backup.

Operation

Mastercycler pro English (EN)

Abb. 5-1: Navigation tree of the Mastercycler pro. The MMC and USB nodes are only visible if a storage medium has been inserted.

Fig. 5-1: Navigation tree of the Mastercycler pro. The MMC and USB nodes are only visible if a storage

medium has been inserted.

5.4.2 Using the navigation tree

5.4.2.1 Opening the node

You have three options:

 Mark the node with the arrow keys and press the Enter key or the arrow key .

This opens the node and the plus symbol in front of the node changes to a minus symbol.

 Or click on the plus symbol in front of the node with the mouse.

 Alternatively, use the mouse to double-click on the desired node.

Operation

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5.4.2.2 Closing the node

Carry out the above-mentioned steps in the reverse order:

 Use the mouse to click on the minus symbol in front of the node.

 Alternatively, double-click on the node.

 Or mark the node with the arrow keys and press the Enter key or the arrow key .

This closes the node and the minus symbol in front of the node changes to a plus symbol.

5.4.3 Using external storage media

You can save programs, log files and protocols on an MMC memory card or a USB storage medium and then, for example, archive or print them out on a PC. External storage media are also needed for data backups and software updates.

The status of the USB ports is indicated by the following symbols in the upper right-hand corner of the control panel:

• USB ports deactivated. The USB ports cannot be used if the control panel is connected

to a Mastercycler ep. Connect the control panel to a Mastercycler pro to activate the USB ports.

• USB error. A faulty or incompatible USB device has been connected.

Disconnect all USB devices and switch the control panel off. After restarting, all USB ports will be reactivated.

5.4.3.1 Inserting storage media

Connecting a USB storage medium

 Insert the USB storage medium into a USB port

on the right-hand side of the control panel

(Fig. 2-3 on p. 10). After a few seconds, the USB node appears in the navigation tree. The programs and user nodes saved on the USB storage medium can be accessed via this node.

The control panel creates a folder called \eppendorf on the USB storage medium where all files will be saved.

Inserting an MMC memory card

 Insert the MMC memory card into the MMC slot

on the right-hand side of the control panel

(Fig. 2-3 on p. 10) until it locks into position. After a few seconds, the MMC node appears in the navigation tree. The programs and user nodes saved on the MMC can be accessed via this node.

Operation

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5.4.3.2 Removing storage media

CAUTION! Device damage due to cancellation of the update process.

 Ensure power supply during the update.  Do not switch the cycler off during the update.  Do not disconnect the control panel from the cycler during the update.  Leave the external storage medium in the control panel until the latter has been restarted

after the update and you have logged on again.

Removing the USB storage medium Caution! The symbol is displayed in the upper

right corner while the USB storage medium is being accessed. Wait until it has disappeared before removing the USB storage medium.

 Remove the USB storage medium.

The USB node disappears from the navigation tree. The MMC node disappears from the navigation tree.

Removing the MMC memory card

Caution! The symbol is displayed in the upper

right corner while the MMC is being accessed. Wait until it has disappeared before removing the MMC.

1. Briefly press on the projecting end of the inserted

MMC.

2. Pull the MMC out of the slot.

5.4.4 Selecting export file formats

When exporting programs, protocols and log files to external storage media, you can choose between text-only format (.TXT) and PDF.

Select memory device Select USB or MMC as the storage medium for the file you would like to

export, if more than one storage medium is connected to the system.

Select file format Selecting the file format:

• PDF: Saves the file as PDF. Use this setting if you only want to print or view the documents on your PC. You need to have a PDF reader software installed (e.g. Adobe Reader

• Text-only (.TXT): Saves the file in text-only format (.TXT). Use this setting if you want to process the document content electronically (e.g. embed it in a report).

 Confirm your selection with OK.

The file is copied to the external storage medium, and the file name is displayed.

5.5 Managing folders and programs

If you are logged in as a normal user (not as the administrator), you are only authorized to create, edit and delete programs and folders below your own user node. More information on user rights can be found in a separate section(see Administrator functions on p. 55).

5.5.1 Creating folders and programs

5.5.1.1 Creating folders

Proceed as follows:

1. Mark the user node.

2. Press the New Folder function key.

3. Use the numerical keys on the control panel to enter a name for the new folder.

Instead of the numerical keys, you can also use the software keyboard to make entries in text fields. This is called up with the Keybd function key.

Operation

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4. To enter a comment for the new folder, select the Next key in the Comment field.

5. Enter a comment text using the numerical keys and confirm by pressingOK.

The folder is created and appears in the navigation tree under your user node.

You can change the name and the comment of the folder at any time using theProp. function key.

5.5.1.2 Creating a new program

Proceed as follows:

1. In the navigation tree, open your own user node and possibly the folder in which you want to create the new program.

2. Press the New function key.

Operation

Mastercycler pro English (EN)

The New Cycler Program window is displayed. You can enter a name and a comment for the new program using the numerical keys here.

3. Press the OK function key.

The program is created and appears under your user node.

You can change the name of the program or the comment at any time using the Prop. function key.

To protect a program against inadvertent changes, you can use the Prop. function key to mark it as write-protected (read only). This means the program can then be displayed in the program editor(see p. 41), but not changed. This is recommended when working without a PIN.

5.5.2 Copying folders and programs

You can copy folders and programs to your own user nodes as follows. When copying a folder, the programs it contains are also automatically copied.

1. In the user node belonging to any user, mark the folder or the program you want to copy.

2. Press the Copy function key.

A message appears informing you that the folder or the program was copied to the clipboard.

3. Confirm the note with OK.

4. Mark your own user node. If you want to copy a single program, you can also select a folder below your user node to which you want to insert the program.

5. Press the Paste function key.

If a program or folder already exists with the same name, you will be prompted to enter a new name. The folder or program is inserted under your user node. You can now start or edit this program.

5.5.3 Deleting folders and programs

1. In the navigation tree, mark the folder or the program.

2. Press the Delete key.

A confirmation prompt appears.

3. Confirm confirmation prompt with Yes.

The folder or program is deleted.

Programming

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6 Programming

6.1 Overview of the program editor

The graphic program editor can be used to view and change existing programs.

6.1.1 Opening the program editor

1. Mark the desired program in the navigation tree or create a new program (see Creating a new program on p. 39).

2. Press the Edit function key.

The program editor is displayed.

6.1.2 Structure of the program editor

A program consists of a program header and up to 99 program steps, which are processed successively by the cycler. The program editor features a graphic user interface for intuitive creation and adjustment of user-specific programs.

1 Number of the program step 2 Block temperature [°C]

The thermoblock is heated or cooled in the relevant step at this temperature.

3 Hold time [mm:ss]

The set block temperature is maintained for this time.

4 Average temperature for gradient steps

When using gradient steps, increasing temperatures are generated across the columns of the thermoblock from left to right. The average temperature is displayed here.

5 Program step highlighted marking

The selected program step is highlighted in blue. New program steps are inserted before the marked step.

6 Step number within the cycle

The step number within a cycle and the total number of steps in the cycle are displayed on the top right. Example: 2/3: Step 2 of a 3-step cycle (3

StepCyc).

7 Active input field

Numerical keys are used to make entries.

8 Extended program step

Program steps are indicated by an asterisk* if they are associated with a temperature or holding time increment or with a reduced heating or cooling rate.

9 Marking of gradient steps

Gradient steps are indicated by three horizontal lines.

10 Number of cycles

The cycle number indicates how often the program steps indicated by the green horizontal arrow are to be repeated.

Programming

Mastercycler pro English (EN)

6.2 Defining general settings

Each Mastercycler pro program features a program header in which you can define the block temperature control mode and heated lid behavior. These settings are valid for the entire program.

6.2.1 Opening the header

1. Open the program editor (see Opening the program editor on p. 41).

2. Press the Header function key.

The header settings are displayed.

6.2.2 Editing header settings

Mastercycler Defining the cycler type

• To create a program, which is suitable for all Mastercycler pro devices, selectany (default setting).

• To create a program for a specific Mastercycler pro (e.g. pro S) or Mastercycler ep, select the desired cycler version from the list. The program can then only be started on this type of cycler variant.

Lidtemp. Heated lid temperature (37 °C to 110 °C). The set lid temperature is maintained

constant during the program run. The heated lid remains switched off if temperatures of less than 37 °C are entered.

Programming

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TSP/ESP heated lid

Switch off lid at low block temperature

Temp. mode Block temperature control mode

Preheat heated lid

• Mastercycler pro: Switch TSP on and off. When TSP is activated, the heated lid is heated to the set temperature, while the block is maintained constant at 20 °C. The program is started after the lid temperature has been reached.

• Mastercycler ep: Switch ESP on and off.

When ESP is activated, the heated lid is first heated to the set temperature before it is lowered onto the tube surfaces. The program is started after the heated lid has been lowered.

Lid heater with automatic switch-off

• Activated (default setting): The lid heater switches off if program steps have block temperatures of <15°C.

• Deactivated: The lid heater switches off after the end of the program. If the last command in a program is a hold step, the heated lid remains switched on until the

Enter key is pressed.

Note: This option is useful if you have inserted hold step as the last program step in

order to automatically cool the samples after the PCR.

• block (default setting): The temperature of the thermoblock is directly controlled by the current temperatures measured at the integrated sensors. Use this setting for Fast PCR, for low reaction volumes (< 20 μL) and for templates with a low G/C content. With this setting you achieve the shortest program runtimes.

• safe: Block temperature control mode for difficult templates and large reaction volumes. Use this setting for sample volumes of 50 μL or more or for amplifying samples with a high G/C content. The program runtimes are a bit longer than with thefast setting.

• fast: Block temperature control mode for standard applications.

Use this setting for sample volumes between 20 μL and 50 μL or if theblock setting only results in a weak amplification. The program runtimes are a bit longer than with theblock setting.

Simulate Mastercycler gradient

Impulse This is only displayed if under Mastercycler the pro S or ep gradient S setting were

With this setting it is possible to simulate the temperature control behavior of the Mastercycler 5333 or Mastercycler gradient 5331. This allows you to transfer programs from these cycler models without making adjustments to the Mastercycler pro(see Transferring programs from the Mastercycler 533x on p. 64). If this option is deactivated, Mastercycler 5333/5331 programs will have to be adjusted for the Mastercycler pro due to the different temperature control behavior. This may influence the PCR results.

selected. If this option is activated, a higher heating rate (pulse) is achieved during the first temperature control step. This can reduce non-specific PCR product formation.

Programming

Mastercycler pro English (EN)

6.3 Editing programs

You can add, edit or delete steps in new (see p. 39) or copied (see p. 40) programs.

6.3.1 Inserting a program step

1. Program editor(see Opening the program editor on p. 41) .

2. Use the arrow keys to mark the program step before which the new program step is to be inserted.

The marked program step is highlighted in blue.

3. Press the Insert function key.

A list appears containing the available program steps.

4. Use the arrow keys to select the desired program step.

The following program steps are available:

1StepCyc

to3StepCyc

nStepCyc Cycle program step with an adjustable number of temperature steps. After selecting

Temp Individual temperature step with adjustable block temperature and hold time.

Hold Temperature step with adjustable block temperature and indefinite hold time.

Sound This program step emits an acoustic signal.

Pause Like Hold. The block temperature remains set to the value of the previous temperature

Cycle program step with 1 to 3 temperature steps with an adjustable number of repetitions (1 to 99). With cycle program steps you can increase or reduce the block temperatures and hold times by a preset value after each cycle (see Editing parameters on p. 45).

this program step and confirming by pressingEnter a dialog window appears for the following entries.

• Steps per Cycle: Number of temperature steps per cycle (1 to 20)

• Number of Cycles: Number of cycles (1 to 99). If you have inserted a cycle with

more than 6 steps, use the arrow keys and to display the hidden steps.

The set temperature is maintained until the user presses the Enter key. A corresponding prompt is displayed in the status view and also indicated by the status light flashing orange (see Status lamp on p. 29).

step.

5. Press theInsert function key.

The program step is inserted with standard parameters.

You can now insert additional program steps or edit the parameters of the program steps.

Programming

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6.3.2 Editing parameters

1. Use the arrow keys to mark the desired program step.

The marked program step is highlighted in blue. You can make the settings for temperature, hold time and number of repetitions directly in this view.

Changing the temperature

2. Use the arrow keys to mark the temperature.

The temperature is highlighted in blue.

3. Use the numerical keys to enter a new value between 4.0 °C and 99.0 °C (in increments of 0.1 °C).

4. Confirm by pressing the Enter key.

Changing the hold time

5. Mark the hold time.

The hold time is highlighted in blue.

6. Use the numerical keys to enter a new value between 000:00 to 999:59 (mmm:ss, in increments of 1 s).

7. Confirm by pressing the Enter key.

Changing the number of cycles

8. Mark the value for the repetitions.

The value is highlighted in blue.

9. Use the numerical keys to enter the number of repetitions (1 to 99).

10.Confirm by pressing the Enter key.

You can change other parameters for the marked program step:

Extended options

11.Press the Options function key.

The extended parameter settings for the marked program step are displayed.

Programming

Mastercycler pro English (EN)

Temp Temperature for the program step.

Equivalent to directly editing in the program view.

Temp Inc./Dec. For changing the temperature to the next cycle.

Values ranging from -10.0 °C to +10.0 °C in increments of 0.1 °C are possible. You can switch between positive and negative values by pressing the+/- function key.

Gradient Column temperature gradient in the thermoblock.

Enter the temperature range into thefrom (left-hand side of block) andto (right-hand side of block) fields. TheTemp and Temp Incr. fields are deactivated. To display the temperatures in the individual columns, press the ShowGrad function key. The temperatures displayed there are interpolated approximation values. Maximum gradient range and course depend on the type of block used. To use the extended gradient spread on devices with silver block (Mastercycler pro S and Mastercycler ep gradient S), define the appropriate cycler type in the program header in advance(see p. 42).

TIME Hold time for the program step.

Equivalent to directly editing in the program view.

Time Inc./Dec. For changing the hold time to the next cycle.

Values ranging from -01:00 to +01:00 in increments of 1 s are possible. You can switch between positive and negative values by pressing the +/- function key.

Ramp Temperature control speed of the thermoblock.

Values ranging from 1 % to 75 % and also 100 % are possible.

12.Press theOK function key to accept the settings.

The program view appears.

13.Press the >> function key and then Save.

The changes are saved.

14.Press the Exit function key to exit the editor.

6.3.3 Deleting individual program steps

1. Use the arrow keys to mark the desired program step.

The marked program step is highlighted in blue.

2. Press the Delete key.

3. Press the Save function key to save the change.

Press the Exit function key to exit the editor.

Programming

Mastercycler pro

6.3.4 Deleting all program steps

1. Press the ClearAll function key.

A prompt appears asking whether you want to delete all the program steps.

2. Press the OK function key.

All program steps are deleted.

6.3.5 Exporting a program listing

You can export a program to an external storage medium as a file for documentation purposes.

1. Insert the external storage medium into the control panel.

2. Press the >> function key.

3. Press the Export function key.

4. Select the format (Text or PDF) and confirm with OK.

The program listing is saved to the external storage medium as a file.

English (EN)

6.3.6 Saving a program and exiting the editor

1. Press the >> function key.

2. Press the Save function key.

The program is saved to the internal memory of the control panel.

3. Press the Exit function key.

The navigation tree is displayed. You can now start the program (see Starting the program on p. 51).

Programming

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7PCR run

7.1 Preparation

7.1.1 Selecting the sample tubes

CAUTION! Unsuitable tubes can result in sample material escaping.

Unsuitable tubes may be so severely damaged that sample material is released.

 Use only tubes whose properties meet at least the requirements specified in these

operating instructions.

 This is especially important when working with infectious material.

CAUTION! Damage to heated lid from sharp-edged sample tubes or objects.

 Use only tubes whose properties meet at least the requirements specified in these

operating instructions.

 Do not use any sharp-edged or sharp objects on the heated lid.  Extra caution should be exercised when cleaning so as not to damage the heated lid.

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CAUTION! Temperatures on the inside of the heated lid may be higher.

If well caps consisting of thermally insulating material are used, the temperature on the inside of the heated lid can be significantly higher than the temperature displayed.

 If you are using well caps consisting of thermally insulating material, for example foamed

material, make sure that it is heat-resistant up to at least 140 °C.

The Mastercycler pro can be used with a great variety of sample tube and plate types. Consider the following when selecting sample tubes, plates and lids:

• Only use sample tubes, plates and lids which have a heat resistance of up to approx. 120 °C.

• The sample tubes and plates must fit into the thermoblock bores free from play to achieve a good heat transfer.

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Mastercycler pro English (EN)

7.1.2 Loading the thermoblock

CAUTION! Damage to the thermoblock from incorrect operation.

 Do not fill sample material directly into the thermoblock.

7.1.2.1 Loading a 96 well block

The Mastercycler pro and Mastercycler pro S can be loaded with 0.2 mL single tubes or strips. You can also use a 96-well PCR plate. The following PCR plate types are compatible with the Mastercycler pro:

Semi-skirted PCR plate Skirted PCR plate Non-skirted/unskirted PCR plate

PCR plate with surrounding half-edge.

PCR plate with a surrounding edge.

PCR plate without any surrounding edge.

7.1.2.2 Loading a 384-well block

The Mastercycler pro 384 can be loaded with a 384-well PCR plate.

To seal the PCR plates, you can use adhesive and heat sealing films, lid strips or reusable silicone mats.

A selection of the PCR micro test tubes and lids can be found separately (see Consumables on p. 86).

7.1.3 Sample volume

The liquid level in the micro test tube should not be above the upper edge of the block.

You can use the following standard values for the maximum sample volumes:

• Mastercycler pro and Mastercycler pro S: Maximum 100 μL per single reaction.

• Mastercycler pro 384: Maximum 25 μL per single reaction.

The sample volumes that can actually be used may vary depending on the type of micro test tube used.

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7.2 Starting the program

Start the PCR programs from the editor or the navigation tree.

If the PIN function is activated, you can only start programs which are stored below your user node (see p. 55).

Prerequisites

You are logged in as user and the program to be started is saved.

1. Mark the program in the navigation tree with the arrow keys or open it in the editor.

2. Insert the sample tubes (see Loading the thermoblock on p. 50).

3. Close the heated lid (see Closing the heated lid on p. 27).

(For devices from the Mastercycler ep series with motorized lid, this will be closed automatically when the program is started.)

4. Press the Start key.

If several cyclers are connected, the Cycler Selection dialog appears.

5. Mark the desired cycler.

6. Press the OK function key.

7. Devices from the Mastercycler ep series only: you are prompted to enter the tube type. Select the tube type used: Tubes for individual tubes or Plate for a PCR plate. Press the OK function key.

The program is started and the status view appears.

2 3

4 5

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7.2.1 Status view

The status view is automatically displayed immediately after a program is started. It shows the progress of and information on a running program. The currently active step is displayed as a flashing line.

1 Program path and cycler

2 Remaining runtime

3 Start and end time of the program

4 Block and heated lid temperature

5 Device status

If you have activated the TSP/ESP function in the program header (see p. 42), the heated lid is heated or cooled to the desired temperature before the program run starts. This process is indicated in the status view by the message TSP Heated Lid.

You can exit the status view at any time via the Exit function key to return to the navigation tree and, for example, edit another program or start a program on a different cycler. The program that was already started is not interrupted as a result.

6Cycle number

7 Block target temperature

8 Remaining hold time

9 Information on the current program step

7.2.1.1 Displaying the status view

Proceed as follows:

1. Mark the cycler in the navigation tree.

2. Press the Status function key.

The cycler is indicated in the navigation tree with a green symbol while it executes a program.

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7.3 Interrupting or stopping the program

You can temporarily interrupt the program run in the status view, for instance, to add reagent, or completely stop the program.

7.3.1 Interrupting the program

1. Open status view (see Starting the program on p. 51).

2. Press the Stop key.

The status view shows Paused.

Observe the safety precautions for opening the heated lid (see Opening the heated lid on p. 28).

7.3.2 Continuing the program

PCR run

1. If the heated lid is opened, draw the heated lid forwards over the samples and close the lid.

2. Press the Resume function key.

The status view displays running and the program run is continued.

7.3.3 Stopping the program

 Press the Abort function key.

The status Idle is displayed. The program has been aborted and cannot be continued.

7.4 Using protocols

The Mastercycler pro creates a protocol for each program run where all executed program steps and any interruptions and errors are recorded.

After running a PCR program, you can view the associated protocol on the control panel, save it on an MMC or a USB memory or send it to a printer.

The protocol is overwritten the next time a program is started on the Mastercycler pro.

Prerequisites

The cycler is idling.

1. Mark the desired cycler node in the navigation tree.

2. Press the function key Protocol.

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The protocol is displayed.

3. Use the function keys << and >> to go one page back or forward.

Use the arrow keys on the control panel to move the visible section.

Further functions for saving and printing the protocol are available in the protocol screen.

Saving a protocol Printing a protocol

1. Insert a USB storage medium or an MMC .

2. Press the function key Export.

3. Select Text or PDF file format. The protocol is copied to the external storage medium.

1. Check if the printer is connected correctly and is ready for printing.

2. Press the function key Print.

The protocol is printed.

7.5 Shutting down and switching off the device

Shut down the control panel before you shut down the Mastercycler pro:

1. Mark the Eppendorf orSystem node in the navigation tree.

2. Press the Shutdown function key.

A confirmation message appears.

3. Press Shutdown function key.

The control panel is shut down.

4. Switch off the cycler at the mains power switch.

System management

Mastercycler pro

English (EN)

8 System management

8.1 Administrator functions

The software of the Mastercycler pro differentiates between standard users and the administrator.

The access rights are distinguished as follows:

Procedure Standard user Administrator

Copying programs From every user node in user's

own nodes

Creating, deleting, editing and starting programs

Managing user accounts and setting PINs

Making system settings None All

Performing self test, validation and calibration

Performing backups, restores and software updates

Only programs below user's own node

Only change own user name and PIN

Only self test and validation Self test, validation and

Only backups All

From every user node in all user nodes

All programs

Create, change, delete user accounts. Change PIN of each user.

calibration

ThePIN setting can be used to define whether all users have to log in with their user names and PINs before using Mastercycler pro or whether all users should receive administrator rights (without entering their user names and PINs).

8.2 Managing user accounts

8.2.1 Creating user accounts

• You can only assign a user name once.

• The administrator uses the fixed user name_admin.

You can set up a separate user account for each user:

1. In the navigation tree, mark the Eppendorf node at the very top.

2. Press the NewUser function key.

TheNew User window is displayed.

System management

Mastercycler pro English (EN)

3. Enter the name and PIN for the new user. Repeat the PIN entry in the Confirmation field.

4. Confirm by pressing the OK function key.

The new user account is set up. In the navigation tree, a new user node is displayed with this user name. The user can now log in with his or her PIN at any time.

8.2.2 Editing user accounts

You can subsequently change the user name, the PIN and the comment for an existing user account depending on your user rights (see p. 55):

1. In the navigation tree, mark the user node and press the Prop. function key.

The User Properties window is displayed.

2. Change the settings for the user account and confirm with the OK function key.

8.2.3 Deleting user accounts

To delete a user account, you need to be logged in as the administrator.

When a user account is deleted, the user node selected as well as all its folders and programs are deleted from the navigation tree. Deletion of a user account cannot be undone.

1. In the navigation tree, mark the user node and press the Delete key.

A prompt appears asking you if you are sure you want to delete the user.

2. Confirm by pressing the OK function key.

The user account is deleted.

8.2.4 Changing the PIN

If you work with the PIN function, only the administrator is authorized to make system settings and manage user accounts.

System management

8.2.4.1 Changing the administrator PIN

If you are logged on as an administrator, you can change the administrator PIN at any time.

CAUTION! Loss of data due to misuse or loss of administrator PIN.

The administrator PIN protects the system from undesired access to its configuration and to the stored data of all users.

 Make a note of the administrator PIN and keep it in a safe place. If you lose the

administrator PIN, contact Eppendorf Service.

 Allow access to the administrator PIN only to persons who are allowed to edit the

configuration of the system and who have the knowledge required to do this.

1. Switch on the Mastercycler pro and log in as the administrator.

2. In the navigation tree, mark the System node and press the Prop. function key.

The System Properties window is displayed.

Mastercycler pro

English (EN)

3. Enter the new administrator PIN using the numerical keys on the control panel.

Delete incorrectly entered PINs with the Delete key and repeat the PIN entry.

4. Enter the new PIN in the Confirmation field to confirm again.

5. Confirm with the OK function key.

The new administrator PIN is immediately valid.

8.2.4.2 Changing the user PIN

You can change the PIN for logging into your user account at any time. To do this, proceed as follows.

1. In the navigation tree, mark your own user node and press the Prop. function key.

TheUser Properties window is displayed.

2. Enter the new user PIN with 4 to 8 digits with the numerical keys on the control panel.

Delete incorrectly entered PINs with the Delete key and repeat the PIN entry.

3. Reenter the new PIN in the Confirmation box to confirm it.

4. Confirm by pressing the OK function key.

The new user PIN is immediately valid.

System management

Mastercycler pro English (EN)

8.3 System settings

Open the System node to set the following system settings.

Show free system memory

Exporting the log file

Changing date and time

Change country settings

Activating or deactivating the acoustic signal

Activating or deactivating PINs

 Mark the node System memory and press the function key Show.

The system memory is shown as a red-green marking at the lower end of the display (red for the used memory, green for free memory).

 Press the Show function key again to hide the display.

 Mark the Logfile node and press the Export or the Export 20 function key.

The log file or the last 20 entries of the log file are written to an MMC or USB storage medium. The log file can be displayed using the function key Show.

1. Mark the node Date + Time and press the function key Set.

2. Set date and time and confirm using OK.

The country settings are indicated with the international area code (e.g. 001 for USA). The language (German or English) as well as the date and time format (12 or 24 hour format) are defined in the country settings.

1. Mark the node Country Settings and press the function key Set.

2. Select the required settings and confirm using OK. The Mastercycler pro is immediately shut down. The modified settings apply after the next start.

 Mark the node Beeper and activate or deactivate it.

If the red checkmark is set, the acoustic signal for error messages and key presses is activated.

Use the PIN node to define whether or not a PIN entry is required for working.

• Mark the node PIN and activate or deactivate it.

The control panel is immediately shut down. The modified settings apply after the next start.

System management

Mastercycler pro

English (EN)

Performing a software synchronization

Exporting user-related data

Use the update node to perform a software synchronization between the cycler and the control panel. The software synchronization may be necessary if you receive error messages after updating the software of the control panel.

1. Highlight Update node.

2. Press Cycler function key.

3. Select the Cycler.

4. Press Start.

Use the UserLog node to export or display all the actions that were performed on the control panel as a log file. You can save login and logout information, all changes and interventions in a running program related to specific users.

 Mark UserLog and press the desired function key:  Show: Display user log. You can print or export the user log in the display.  Exp+Clear: Transfer the user log to the MMC or USB storage medium and delete

the log from the control panel afterwards.

 Export: Copy the user log to the MMC or USB storage medium.

System management

Mastercycler pro English (EN)

8.4 Cycler functions

In the Cycler node, you can view and adjust the status of the thermoblock and the heated lid. You can also retrieve the protocol of the last PCR program used.

Prop. Display device properties.

Incubate Control the thermoblock temperature (4.0°C to 99.0°C).

Lid Control the heated lid temperature (37°C to 110°C).

Status Switch to status view while the program is running.

Protocol Display, export or print out protocol of the last PCR program processed.

The protocol contains a record of the temperature curve and the device settings. The previous protocol is overwritten whenever a new program is started on the cycler.

8.4.1 Cycler system functions

The System node contains the cycler system level with the following function keys.

Block Display block type, serial number as well as the date when the last validation and

calibration took place.

Restart Entry for maximum duration of interruption of voltage supply (mm:ss) after which a

running program should be automatically restarted. The program is not restarted after a longer interruption.

Sensors Current sensor temperatures for the lid and block.

Validation Check block temperature control with the temperature validation system.

Calibration Calibrate and adjust block temperature control with the temperature validation

system. Only displayed if the administrator is logged in.

Using the functions Validation and Calibration requires the Eppendorf temperature validation system (see Accessories on p. 85). For information on how to use this system, refer to the corresponding operating manual.

System management

Mastercycler pro

English (EN)

8.4.2 Self test

The self test function of the Mastercycler pro allows you to perform a quick check regarding the following attributes of the thermoblock without requiring an external measurement system:

• Heating and cooling rates

• Proper functioning of the temperature control circuit

• Temperature homogeneity in each of the three zones of the block and across the entire block

After a successful self test, a certificate can be generated in PDF format.

A separately available USB key is required for performing the self test (see Accessories on p. 85). Please refer to the documentation of the self test USB key for a detailed description of the self test function.

8.5 Managing and backing up data

8.5.1 Backing up data

The function keys in the above view show the options available to the administrator for transferring data. Standard users only have the option of performing backups here.

 Insert an MMC memory card or a USB storage medium into the right-hand side of the control panel.

The node MMC or USB will be displayed in the navigation tree.

 Select the MMC or USB node.

System management

Mastercycler pro English (EN)

Backup Back up user nodes, folders and programs onto an external storage medium.

• MMC: The MMC inserted will automatically be completely deleted at the start of the backup process.

• USB: Any files and folders in the directory \eppendorf will be deleted automatically at the start of the backup process.

The backed-up data can be copied into the control panel using the Restore function.

Restore Administrator only: copy the user nodes, folders and programs saved on the external

storage medium during a backup to the control panel. This process deletes any user nodes, folders and programs which already exist in the control panel. After the restore process, only the data copied from the external storage medium will be available.

8.5.2 Exporting and importing programs

Export

Importing programs and folders

1. In the navigation tree, mark the program.

2. Insert an MMC or a USB storage medium into the slot on the right-hand side of the control panel.

3. Press the Export . The program is copied to the MMC or the USB storage medium.

The exported programs can be transferred to a different control panel.

1. Insert an MMC or a USB storage medium into the slot on the right-hand side of the control panel.

2. Open the node MMC or USB and mark the folder or program you would like to import.

3. Press the function key Copy.

4. Mark the user node or folder in the destination and press the function key Paste.

8.6 Performing a control panel software update

CAUTION! Device damage due to cancellation of the update process.

after the update and you have logged on again.

• Synchronize all connected Cyclers after the software update.

• Create an up-to-date backup immediately after an update.

8.6.1 Performing an automatic software update

System management

Mastercycler pro

English (EN)

The auto update function automatically initiates a software update on the control panel.

Prerequisites

• You are logged on as an administrator or the PIN function is switched off.

• All connected cyclers are idling.

• The navigation tree is displayed.

1. Log on as administrator or deactivate the PIN function.

2. Insert MMC memory card or USB storage medium with the new software version.

3. Confirm query with Yes.

The software version currently installed and the software version on the storage medium are displayed.

4. Press the Update function key to start the update. You can cancel the operation using the Restart function key.

The update may take several minutes. Afterwards, the control panel will automatically restart.

8.6.2 Performing a manual software update

If the automatic update was canceled or did not start, you can also perform a manual update.

Prerequisites

• You are logged on as an administrator or the PIN function is switched off.

• An MMC memory card or a USB storage medium with a newer software version is inserted into the control panel.

1. Highlight MMC node or USB.

2. Press Firmware function key.

Always complete the software update for the control panel first, and then the update for all connected Cyclers (software synchronization).

System management

Mastercycler pro English (EN)

3. Select Software Update Control Panel entry and press the Start function key.

The software version currently installed and the software version on the storage medium are displayed.

4. Press the Update function key to start the update. You can cancel the operation using the Restart function key.

The update may take several minutes. Afterwards, the control panel will automatically restart.

8.7 Transferring programs from the Mastercycler 533x

PCR programs established on the Mastercycler (5333) or the Mastercycler gradient (5331) can easily be transferred to the Mastercycler pro without first having to optimize the temperatures and hold times.

 Enter the program into the Mastercycler pro editor:

Example

1 T = 94.0° 0:02:00

2 T = 94.0° 0:00:15

3 T = 60.0° 0:00:15

+0.0° +0:00

R = 3.0°/s +0:0°/s

G =10.0°

4 T = 72.0° 0:00:30

5 GOTO 2 REP 29

6 T = 72.0° 0:02:00

7 HOLD 4.0° ENTER



Make the settings in the header(see Editing header settings on p. 42).

System management

Mastercycler pro

English (EN)

Setting from the Mastercycler 533x program

LID 105 °C Lidtemp 105 °C

Wait

AUTO

CNTRL

Block temperature control behavior Activate theSimulate Mastercycler gradient checkbox

Corresponding setting in the program header

TSP/ESP heated lid

Switch off lid at low block temperature

BLOCK =Temp. mode block TUBE = Temp. mode safe orfast

 Press the OK function key.

The editor is displayed.

 Press the >> function key and then Save.

You can start the program without renewed optimization of the parameters.

System management

Mastercycler pro English (EN)

9 Quick start

Enter

Delete

abc

jkl mno

tuv wxyz

def

pqrs

ghi

Enter

9.1 Login

1. Open list.

2. Select user.

Quick start

Mastercycler pro

English (EN)

3. Confirm selection.

4. Go to the PIN field.

5. Enter and confirm PIN.

Delete

abc

jkl mno

tuv wxyz

def

pqrs

ghi

Enter

Quick start

Mastercycler pro English (EN)

9.2 Creating folders and programs

1. Select the user or folder.

2. Press NewFolder orNew.

3. Enter and confirm names and comments.

9.3 Copying folders and programs

1. Select the folder or program.

2.Press the Copy function key.

3. Confirm selection.

4. Mark the target folder or user.

5. Press Paste.

9.4 Deleting folders and programs

Delete

EnterEnter

Mastercycler pro

1. Mark the folder or program.

2. Press the Delete key.

3. Confirm the selection.

Quick start

English (EN)

9.5 Editing programs

1. Select the program.

2. Open the program editor.

3.Press the Header function key.

6. Set the lid functions and control mode.

7. Confirm input.

Delete

abc

jkl mno

tuv wxyz

def

pqrs

ghi

Delete

abc

jkl mno

tuv wxyz

def

pqrs

ghi

Enter

Quick start

Mastercycler pro English (EN)

8. Press the Insert function key.

9. Select the program step.

10. Enter the temperatures, hold times and cycle number.

11. Select the program step.

12. Press the Options function key.

13. Set the gradient, increment and ramp.

14. Confirm input.

15. Press the >> function key.

16. Press the Save function key.

17. Press the Exit function key.

9.6 Stopping and starting programs

Enter

Start

Quick start

Mastercycler pro

English (EN)

1. Mark the program.

2. Insert the sample tubes.

3. Close the heated lid.

4. Start the program.

5. With several connected cyclers: Mark the cycler.

6. Confirm input.

9.7 Switching off the device

7. You can use the status view to carry out the following program commands

• Stop

• Abort

• Resume

1. Mark the Eppendorf or System node.

2. Press the Shutdown function key.

3. Switch off the Mastercycler pro at the mains power switch.

Quick start

Mastercycler pro English (EN)

10 Maintenance

10.1 Cleaning

DANGER! Electric shock.

 Switch off the device and disconnect the power plug before opening the device,

performing work on electrical connections or starting maintenance or cleaning work.

WARNING! Burns from hot thermoblock and heated lid.

The thermoblock, the inner side of the heated lid and micro test tubes/PCR plates can reach temperatures of over 50 °C very quickly.

that the temperature on the inside of the heated lid can be significantly higher than the temperature displayed.

 Before starting cleaning wait for the thermoblock and heated lid to cool down.

Maintenance

Mastercycler pro

English (EN)

WARNING! Injury from sharp-edged components.

When the Mastercycler pro is in the open position, sharp-edged components are exposed. When cleaning the Mastercycler pro, the sharp edges on the rollflex cable and the guide rail may cause injuries.

 Take special care and wear protective gloves.

WARNING! Injury from trapping or crushing.

 Do not place your fingers between the lid and the housing when opening and closing the

device or when opening it into the cleaning position.

 Do not reach in under the open lid.  Take care that the stand is properly engaged so that the lid stays in the opened position.

CAUTION! Damage to heated lid from sharp-edged sample tubes or objects.

 Use only tubes whose properties meet at least the requirements specified in these

operating instructions.

 Do not use any sharp-edged or sharp objects on the heated lid.  Extra caution should be exercised when cleaning so as not to damage the heated lid.

Maintenance

Mastercycler pro English (EN)

CAUTION! Caution when using aggressive chemicals.

Aggressive chemicals may damage both the device and its accessories.

 Do not use any aggressive chemicals on the device and accessories such as strong or weak

bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.

 If the device becomes contaminated with aggressive chemicals, clean it immediately with a

neutral cleaning agent.

We recommend that you regularly clean the surfaces of the Mastercycler pro.

10.1.1 Cleaning the housing

 Clean the surfaces of the Mastercycler pro with a damp, lint-free cloth. Use a neutral lab cleaner if

necessary.

Instructions on disinfecting and decontaminating the device are described separately(see Disinfection/ decontamination on p. 76).

10.1.2 Cleaning the heated lid

10.1.2.1 Moving the heated lid into cleaning position

1. Push the heated lid into rear position.

2. To open the lock, press the left and right latches underneath the front edge of the heated lid upwards (1).

3. Fold up the heated lid (2) until the stand (4) is locked.

Maintenance

3 4

Mastercycler pro

English (EN)

1 Lid release latches 2 Heating unit

3 Rollflex cable 4Stand

4. Remove any contamination in the area of the block and on the inner side of the lid with a damp, lint-free cloth. Use a neutral lab cleaner if necessary. Remains of markings from tube lids on the heating unit can be removed with ethanol or isopropanol.

Keep the block bores free of dust and lint. Even small amounts of deposits can affect the heat transfer between the block and sample tubes.

10.1.2.2 Moving the lid out of the cleaning position

1. While holding the front edge of the lid with your right hand, pull the stand 1forwards with your left hand.

2. Lower the heated lid slowly until it locks into the horizontal position.

Maintenance

Mastercycler pro English (EN)

10.2 Disinfection/decontamination

DANGER! Electric shock as a result of penetration of liquid.

 Switch off the device and disconnect it from the power supply before starting cleaning or

disinfecting.

 Do not allow any liquids to enter the inside of the housing.  Do not perform spay disinfection.  Only reconnect the device to the power supply once it is completely dry.

CAUTION! Material damage from UV and other high-energy radiation.

 Do not use UV, beta, or gamma radiation or any other high-energy radiation source for

disinfecting.

Required equipment

• Alcohol (ethanol, isopropanol) or disinfectants containing alcohol

• Mild, soap-based household cleaning agent

Proceed as follows:

1. Select a disinfection method that complies with the legal regulations and guidelines applicable to your range of application.

2. Switch off the device and disconnect it from the power supply.

3. Wipe down all parts of the device and accessories, including the connecting cable, with the disinfectant.

4. Clean the device with a mild soap-based household cleaning agent (see Cleaning on p. 73).

10.3 Decontamination before shipment

If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following:

WARNING! Risk to health from contaminated device

1. Follow the instructions in the decontamination certificate. It is available in PDF format on our homepage(www.eppendorf.com/decontamination)

2. Decontaminate all the parts you want to dispatch.

3. Enclose the fully-completed decontamination certificate for returned goods (incl. the serial number of the device) with the dispatch.

Troubleshooting

Mastercycler pro

English (EN)

11 Troubleshooting

11.1 General errors

Technical faults may be triggered by faults such as a power failure or power fluctuations. In most cases it is therefore sufficient simply to switch the device off and back on again after about 10 seconds. If the fault occurs again, please note the fault code (e.g. 0x0B04) and inform Eppendorf Service.

11.2 Error messages

Symptom/message Cause Remedy

0x0108 0x2800 to0x2802

0x0140 Incompatible or defective mouse

0x0709 0x070A 0x070B

0x0D1D 0x0D1E 0x2600 to0x260F

0x0D25 0x0D26 0x2900 to0x2906

0x0DA1 0x0DA2

The software versions of the control panel and the cycler are not compatible.

Damaged file found during software update.

Incompatible or defective printer, incorrect printer settings, loose cable connection.

Communication problem with the temperature validation system.

Incorrect lid status.

 A software synchronization is needed.

 Check the plug connection of the

mouse or replace the mouse.

 Download the software update packet

(ZIP archive) again from the Eppendorf homepage and unpack it on an empty MMC.

 Replace the MMC.

 Check the cable connection between

the cycler and the printer.

 Replace the printer cable.  Check the printer status (e.g. paper

blockage, offline).

 Check cables between the cycler and

the temperature validation system, using the temperature validation system documentation as a reference.

 Make sure that the lid is fully in the

front position at the program start and is not wedged.

 Insert the sample tubes into the block

before the program start.

If further error messages appear:

1. Switch off the Mastercycler pro.

2. Check all cable connections.

If the fault occurs again after switching on the device, please note the fault code and contact Eppendorf Service.

Troubleshooting

Mastercycler pro English (EN)

12 Transport, storage and disposal

12.1 Packing

WARNING! Risk of injury when lifting and transporting the device.

Lifting the device with the wrong posture may cause serious damage to your health. If the device falls to the ground it can cause injuries.

CAUTION! Injury caused by breakage or slipping of packing materials.

If the device falls to the ground it can cause injuries.

 Always hold the device at the base of the housing when lifting it.  Do not lift the device by the packing materials.

Transport, storage and disposal

Mastercycler pro

English (EN)

CAUTION! Damage as a result of incorrect packing.

Eppendorf accepts no warranty or liability for damage caused by incorrect packing.

 Only dispatch the device in the original packaging provided for transport.

Use the supplied packing material when packing the device for transport or storage. If you do not have this anymore or if it is damaged, contact your Eppendorf office.

The packaging of the device consists of:

• 1 plastic bag for wrapping the device

• 1 foam bottom part

•1 foam lid part

• 1 carton consisting of bottom, lid and insert for small parts

• 4 plastic locking clips

• 1 empty PCR plate

Transport, storage and disposal

Mastercycler pro English (EN)

Proceed as follows:

1. Pay attention to the decontamination notes (see Decontamination before shipment on p. 76).

2. Switch off the device and open the heated lid.

3. Let the device stand for 30 minutes and ensure that there is no condensate in the thermoblock.

4. Disconnect the device from the power supply.

5. Disconnect all the connections from the rear of the device and remove the control panel.

6. Load the thermoblock with an empty PCR plate.

7. Close the heated lid.

8. Pack the device into the plastic bag.

9. Place the foam bottom part onto the bottom of the packaging.

10.Hold the device at the front and back of the lower part of the housing and lift it into the foam bottom part.

11.Place the foam lid on top of the device.

12.Close the carton lid and insert the four plastic locking clips into the cut-outs on the side of the carton.

13.For fastening, push in the flaps of the plastic locking clips.

12.2 Transport

 Use only the original packaging for transport.

The transport and storage conditions are described separately(see Ambient conditions on p. 83).

Transport, storage and disposal

Mastercycler pro

English (EN)

12.3 Disposal

In the event of disposing of the product, please observe the applicable legal regulations.

Information on the disposal of electrical and electronic devices in the European Community

The disposal of electrical devices is regulated within the European Community by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).

In accordance with this, any devices delivered after 13/08/2005 on a business-to-business basis, which includes this product, may no longer be disposed of in household waste. To document this they have been marked with the following identification:

As disposal regulations may differ from one country to another within the EU please contact your supplier, if necessary.

Transport, storage and disposal

Mastercycler pro English (EN)

13 Technical data

13.1 Power supply

Voltage 100 to 130 V

200 to 240 V

Frequency 50 Hz to 60 Hz

Power consumption 950 W

Overvoltage category II (IEC 610 10-1)

Degree of contamination: 2

Protection class 1

13.2 Weight/dimensions

Technical data

Mastercycler pro

English (EN)

Dimensions (W x D x H) 26.0 cm × 41.5 cm × 37.0 cm

(without control panel)

26.0 cm × 50.0 cm × 44.5 cm (with lid in cleaning position) Depth with the control panel connected: + 14 cm.

Weight 18.5 kg (without control panel)

13.3 Ambient conditions

General operation +15 °C to +35 °C

to 70 % rel. humidity up to 2000 m above sea level (approx. 80 kPa)

Storage conditions -20 °C to +70 °C

to 85 % rel. humidity

Technical data

Mastercycler pro English (EN)

13.4 Application parameters

Parameter Mastercycler pro Mastercycler pro S Mastercycler pro 384

Sample capacity 96 x 0.2 ml PCR tubes or a 96-well PCR plate

(unskirted, semi-skirted, skirted; according to SBS standard)

Tempera ture c ontrol range of the block

Gradient range 1 °C to 20 °C 1 °C to 24 °C 1 °C to 20 °C

Temperature range of the gradient

Tempera ture c ontrol range of the lid

Block homogeneity *

35 °C 90 °C

Control accuracy* ±0.2 °C

Heating rate** approx. 4 °C/s approx. 6 °C/s approx. 4 °C/s

Cooling rate** approx. 3 °C/s approx. 4.5 °C/s approx. 3 °C/s

4°C to 99°C

30 °C to 99 °C

37 °C to 110 °C

≤ ±0.3 °C

≤ ± 0.4 °C

one 384-well PCR plate

* with deactivated gradient function

** measurements on the block

13.5 Control panel

Number of Mastercycler pro devices that can be controlled the control panel

Number of programs > 700

Number of folders > 100

Max. number of cycles 99 (1 to 20 temperature control steps per cycle)

Dimensions (W x D x H) 25 cm x 16.5 cm x 11 cm

Weight 1.7 kg

Connections 2× USB for mouse, printer, USB storage medium

Power source/consumption Occurs over connected Mastercycler pro

1 to 5

and self test USB key 1× MMC slot

14 Ordering Information

14.1 Mastercycler pro

Ordering Information

Mastercycler pro

English (EN)

Order no. (International)

6321 000.019 - 230 V / 50 - 60 Hz 6321 000.027 950030010 120 V / 50 Hz, with US- plug

6325 000.013 - 230 V / 50 - 60 Hz 6325 000.021 950030020 120 V / 50 Hz, with US-plug

6324 000.010 - 230 V / 50 - 60 Hz 6324 000.028 950030030 120 V / 50 Hz, with US-plug 6320 000.007 950030050 Control Panel

Order no. (North America)

Description

Mastercycler pro

Mastercycler pro S

Mastercycler pro 384

14.2 Accessories

Use only genuine Eppendorf accessories or accessories approved by Eppendorf.

Order no. (International)

6320 071.001 950030040 For Mastercycler pro and Mastercycler nexus

5341 612.006 950014008 50 cm 5341 611.000 950014016 150 cm 5075 780.003 960002008 MultiMediaCard

0055 000.298 950008059 for Mastercycler, Mastercycler ep, Mastercycler pro and

5331 233.007 on request Sensor for 384 well block

5349 810.001 950017007 CD incl. instructions and connection cabel 5349 820.007 950017202 CD incl. instructions

Order no. (North America)

Description

Selftest USB Key

CAN-Bus cable

connecting cable between 2 thermomodules

Temperature Verification System – Single-channel

Mastercycler nexus, Thermomixer compact, Thermomixer comfort, Thermostat plus

CycleManager pro

Ordering Information

Mastercycler pro English (EN)

14.3 Consumables

Order no. (International)

0030 128.648 951020401 clear

0030 128.575 951020303 standard profile, clear

0030 133.307 0030133307 low profile, clear 0030 133.358 0030133358 low profile, divisible, clear 0030 133.366 0030133366 standard profile, clear 0030 133.374 0030133374 divisible, clear

0030 129.300 0030129300 clear

0030 129.326 0030129326 clear

0030 129.504 0030129504 clear

0030 129.512 0030129512 clear

0030 129.342 0030129342 clear

0030 128.508 951020702 clear

0030 124.332 951010006 PCR clean, colorless

0030 124.359 951010022 colorless

0030 124.804 0030124804 0.1 mL, without lid, 10 × 12 strips

0030 124.812 0030124812 domed, 10 × 12 strips 0030 124.820 0030124820 flat, 10 × 12 strips

0030 124.839 0030124839 domed, 10 × 12 strips 0030 124.847 0030124847 flat, 10 × 12 strips

Order no. (North America)

Description

twin.tec PCR Plate 96, skirted

low profile, wells colorless, 25 pieces

twin.tec PCR Plate 96, semi-skirted

Wells colorless, 25 pieces

twin.tec PCR Plate 96 unskirted

Wells colorless, 20 pieces

twin.tec microbiology PCR Plate 96 skirted

Wells colorless, 10 pieces

twin.tec microbiology PCR Plate 96 semi-skirted

Wells colorless, 10 pieces

twin.tec PCR Plate 96 LoBind semi-skirted

wells colorless, 25 pieces

twin.tec PCR Plate 96 LoBind skirted

wells colorless, 25 pieces

twin.tec microbiology PCR Plate 384

Wells colorless, 10 pieces

twin.tec PCR Plate 384

Wells colorless, 25 pieces

PCR Tubes 0,2 mL

1,000 pieces

PCR Tube Strips 0,2 mL

10 × 12 strips

PCR Tube Strips

PCR Tube Strips + Cap Strips

Cap Strips

Ordering Information

Mastercycler pro

English (EN)

Order no. (International)

0030 127.838 0030127838 100 pieces

0030 127.854 0030127854 100 pieces

0030 127.781 0030127781 adhesive, 100 pieces

0030 127.790 0030127790 adhesive, 100 pieces

The twin.tec PCR plates are also available in various frame colors (red, yellow, green, blue). The complete range of PCR consumables can be found in the Eppendorf catalog or at www.eppendorf.com

Order no. (North America)

Description

Heat Sealing PCR Film

Heat Sealing PCR Foil

PCR Film

PCR Foil

14.4 Recommended auxiliary materials

Order no. (International)

0030 124.235 951010031 Set of 10

0030 124.243 951010049 Set of 5

0030 127.153 951023108 1 piece

5390 000.024 - 230 V / 50 Hz 5390 000.016 951023078 115 V / 60 Hz

5390 100.029 951023086 for 384-well PCR plate

3881 000.023 022510541 pink 3881 000.031 022510525 blue

0030 127.455 951010065 10 pieces

Order no. F(North America)

Description

Work tray

for 96 × 0.2 mL PCR tubes, 5-/8-PCR tube strips and plates without border

Frame for work tray

Capping Aid

Heat Sealer

Base Plate

PCR-Cooler

PCR Rack

Index

Mastercycler pro English (EN)

Index

1 … 3 StepCyc (program step)..............................44

Access rights .........................................................55

Address display .....................................................29

Administrator functions.........................................55

Alarm

Activate ............................................................58

Deactivate ........................................................58

Auto-Restart ..........................................................60

Auto-update of the control panel software............63

Backup...................................................................62

Beeper ...................................................................58

block (header setting)............................................43

Date + Time .......................................................... 58

Decontamination................................................... 76

Define administrator PIN...................................... 31

Desinfection.......................................................... 76

Disposal ................................................................ 81

Export ............................................................. 62, 62

Program listing................................................ 47

fast (header setting).............................................. 43

Folder

Import.............................................................. 62

Folders

Changing the comment ................................... 39

Changing the name ......................................... 39

Copy ................................................................ 40

Delete .............................................................. 40

New ................................................................. 39

Change country settings........................................58

Changing the date .................................................58

Changing the time .................................................58

Cleaning

Heated lid.........................................................74

Housing............................................................74

Confirmation..........................................................57

Connect the control panel

Connecting multiple cyclers ............................23

Connecting the mouse .....................................26

to the Mastercycler pro....................................23

Connecting the mouse...........................................26

Connecting the printer ..........................................26

Control Panel

Keys..................................................................30

Country Settings....................................................58

Gradient .......................................................... 41, 46

Guest

User ................................................................. 34

Heated lid

Address display ............................................... 29

Cleaning position............................................. 74

Close................................................................ 27

Control temperature (manually)...................... 60

Open ................................................................ 28

Status lamp...................................................... 29

Switch lid heater off automatically.................. 43

Temperature control before program start...... 43

Hold (program step) ............................................. 44

Holding time increment........................................ 41

Index

Mastercycler pro

English (EN)

Impulse..................................................................43

Incubate.................................................................60

Keys.......................................................................30

Log file

Display .............................................................58

Export...............................................................58

Logout ...................................................................34

Mains connection ..................................................22

Mini-satellite system

Networking ......................................................13

Set-up...............................................................23

MMC

Insert ................................................................37

Labware ...........................................................38

Use ...................................................................37

Navigation tree......................................................35

Networking

Connecting Mastercycler pro to the

Mastercycler ep................................................25

Mini-satellite system ..................................13, 23

PC control ........................................................13

n StepCyc (program step)......................................44

Deactivate........................................................ 58

Define .............................................................. 31

Program

Change comment ............................................ 40

Continue .......................................................... 53

Copy ................................................................ 40

Delete .............................................................. 40

Edit header ...................................................... 42

Export.............................................................. 62

Import.............................................................. 62

Interrupt .......................................................... 53

New ................................................................. 39

Rename............................................................ 40

Restore ............................................................ 62

Save ................................................................. 62

Start ................................................................. 51

Stop ................................................................. 53

Transfer from the Mastercycler 533x.............. 64

Write protection .............................................. 40

Program editor

Description ...................................................... 41

Editing parameters.......................................... 45

Exit .................................................................. 47

Export program listing .................................... 47

Extended options............................................. 45

Header............................................................. 42

Insert step........................................................ 44

Open ................................................................ 41

Save program .................................................. 47

Set gradient ..................................................... 46

Set holding time increment............................. 46

Set temperature control speed........................ 46

Set temperature increment ............................. 46

Program header

Define cycler type............................................ 42

Impulse............................................................ 43

Set lid temperature.......................................... 42

Simulate Mastercycler gradient ...................... 43

Switch off lid at low block temperature .......... 43

Temp. mode..................................................... 43

TSP/ESP heated lid.......................................... 43

Pause (program step) ............................................44

PDF........................................................................38

Activate ............................................................58

Administrator ...................................................31

Change .............................................................57

Protocol

Display............................................................. 53

Print................................................................. 54

Save ................................................................. 54

Index

Mastercycler pro English (EN)

Restart ...................................................................60

Restore ..................................................................62

safe (header setting)..............................................43

Safety instructions

Meaning of symbols...........................................7

Sample tubes .........................................................49

Sample volume ......................................................50

Select Location ......................................................22

Self test..................................................................61

Show system memory ...........................................58

Simulate Mastercycler 5333/5331.........................43

Software

Synchronization ...............................................59

Update control panel .......................................63

Update Cycler ..................................................59

Sound (program step) ...........................................44

Space requirements...............................................22

Status lamp............................................................29

Status view ............................................................52

Switch ESP on and off ...........................................43

Switch lid heater off automatically........................43

Switch off device ...................................................54

Switch TSP on and off ...........................................43

System memory.....................................................58

Thermoblock

Calibration and adjustment ............................. 60

Control temperature (manually)...................... 60

Equipment ....................................................... 50

Sample volume ................................................ 50

Self test............................................................ 61

Validate............................................................ 60

Time increment..................................................... 46

TXT ....................................................................... 38

Unpacking............................................................. 21

Update control panel software.............................. 63

USB

Connecting a storage medium ........................ 37

Connecting the printer .................................... 26

Ports ............................................................... 25

Removing the storage medium ....................... 38

Use storage media........................................... 37

User

Change ............................................................ 33

Logout ............................................................. 34

User accounts

Access rights ................................................... 55

Create .............................................................. 55

Delete .............................................................. 56

Edit .................................................................. 56

User log

Display............................................................. 59

Export.............................................................. 59

Exporting and importing................................. 59

System settings .....................................................58

Temp (program step).............................................44

Temp. mode...........................................................43

Temperature control speed .............................41, 46

Temperature increment...................................41, 46

Text file format ......................................................38

Índice

Mastercycler pro

Español (ES)

Manual de instrucciones Mastercycler proveren pág.Fig.Tab .en pág.

Español (ES)Manual de instrucciones

Índice

1 Descripción del producto. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

1.1 Ilustración general . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

2 Instrucciones generales de seguridad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

2.1 Uso de acuerdo con lo previsto. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

2.2 Requerimiento para el usuario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

2.3 Indicaciones respecto a la responsabilidad del producto. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

2.4 Peligros durante el uso previsto . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

2.5 Símbolos de advertencia en el aparato . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

3 Manejo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

3.1 Primeros pasos . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

3.1.1 Utilizar la tapa térmica vapo.protect . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

3.1.2 Teclas del panel de control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

4 Acceso rápido . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

4.1 Login . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

4.2 Crear directorios o programas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106

4.3 Copiar directorios y programas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106

4.4 Borrar directorios y programas. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

4.5 Editar un programa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

4.6 Arrancar y detener el programa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109

4.7 Desconectar el aparato . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109

5 Mantenimiento . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

5.1 Limpieza . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

5.1.1 Limpieza de la carcasa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

5.1.2 Limpieza de la tapa térmica . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

5.2 Desinfección/descontaminación . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

Índice

Mastercycler pro Español (ES)

1 Descripción del producto

1 2 3 4

1.1 Ilustración general

Abb. 1-1: Mastercyclerpro - Vista anterior y posterior

Descripción del producto

Mastercycler pro

Español (ES)

Imag. 1-1: Mastercycler pro - Vista anterior y posterior

1 Asa de la tapa

Abrir y cerrar así como bloquear y desbloquear la tapa térmica.

2 Tapa térmica vapo.protect

3 Indicación de la dirección

4 Luz indicadora de estado

5 Placa trasera con ranuras de ventilación

6 Hembrilla de conexión a la red

7 Interruptor de alimentación

0 = desconectado, I = conectado.

8 Regleta de terminales

Véase el dibujo de detalle (Fig. 1-2 en pág. 94).

9 Estribo de sujeción

para panel de control.

10 Termobloque

11 Placa de características

Printer RS 232 Control Panel CAN_out CAN_in Term Address

1 2 3 4 5 6 7

1 2

3 4

Descripción del producto

Mastercycler pro Español (ES)

Abb. 1-2: Regleta de terminales

Imag. 1-2: Regleta de terminales

1 Interfaz paralela

Conexión para impresora.

2 Interfaz serial (RS 232)

Conexión para el sistema de validación de temperatura Eppendorf.

3 Conexión del panel de control

4CAN out

Salida de datos para la conexión de varios Mastercycler pro o Mastercycler ep.

Abb. 1-3: Panel de control

5CAN in

Entrada de datos para la conexión de varios Mastercycler pro o Mastercycler ep.

6 Interruptor "Term"

Terminación del bus CAN en caso de interconexión de varios Mastercycler pro o Mastercycler ep. Posición*inferior: ON. Posición*superior: OFF.

7 Conmutador rotativo de dirección

Ajuste de la dirección del aparato en caso de interconexión de varios Mastercycler pro o Mastercycler ep.

Imag. 1-3: Panel de control

1Indicación

2Conexión USB1

Para ratón, impresora o soporte de datos.

3Conexión USB2

Para ratón, impresora o soporte de datos.

4 Ranura para tarjeta MMC

Para el intercambio de datos vía tarjeta MMC.

Conecte únicamente aparatos que correspondan a las normas IEC 950/EN 60950 (UL 1950) en las interfaces del Mastercycler pro y del panel de control.

Instrucciones generales de seguridad

Mastercycler pro

Español (ES)

2 Instrucciones generales de seguridad

2.1 Uso de acuerdo con lo previsto

El Mastercycler pro está previsto para el uso en laboratorios de investigación y de trabajos de rutina de las ciencias de la vida.

El Mastercycler pro sirve para la temperación de soluciones, suspensiones o emulsiones acuosas dentro de tubos de reacción cerrados para reacciones encimáticas, típicamente para reacciones en cadena de la polimerasa (PCR).

El Mastercycler pro ha sido concebido exclusivamente para uso en espacios interiores.

2.2 Requerimiento para el usuario

Este aparato sólo está previsto para ser utilizado por personal especializado debidamente formado.

Lea atentamente las instrucciones de uso y familiarícese con el modo de funcionar del aparato antes de trabajar con él por primera vez.

2.3 Indicaciones respecto a la responsabilidad del producto

En los siguientes casos pueden verse mermadas las garantías previstas para el aparato. Entonces, la responsabilidad del funcionamiento del aparato recae en el operador:

• El aparato no es utilizado de acuerdo con lo especificado en el manual de instrucciones.

• El aparato es utilizado fuera del ámbito de aplicación descrito.

• El aparato es utilizado con accesorios o consumibles (p. ej. recipientes y placas) que no han sido recomendados por Eppendorf.

• El aparato es mantenido o reparado por personas que no han sido autorizadas por Eppendorf AG.

• El usuario efectúa modificaciones en el aparato sin autorización.

2.4 Peligros durante el uso previsto

Antes de que utilice el Mastercycler pro, debe leer primero las instrucciones de uso y observar las siguientes indicaciones generales de seguridad.

Instrucciones generales de seguridad

Mastercycler pro Español (ES)

¡PELIGRO! ¡Peligro de explosión!

 No ponga en marcha el aparato en espacios donde se trabaje con sustancias con riesgo de

explosión.

 No utilice este aparato para procesar sustancias explosivas, radioactivas o de reacción

violenta.

 No procese en este aparato sustancias que puedan originar una atmósfera explosiva.

¡PELIGRO! Peligro en la manipulación de líquidos tóxicos, marcados radioactivamente o que contengan gérmenes patógenos.

 Respete las disposiciones nacionales relativas al manejo de este tipo de sustancias.  Consulte el "Laboratory Biosafety Manual" (fuente: World Health Organisation, Laboratory

Biosafety Manual, en la versión actualmente vigente) para informarse ampliamente sobre las disposiciones relativas al manejo de gérmenes o de material biológico del grupo de riesgo II o superior.

¡ADVERTENCIA! Peligro para la salud y peligro biológico en caso de funcionamiento con tapa abierta.

Las tapas de los envases pueden abrirse bruscamente a temperaturas altas del termobloque.

 Cierre la tapa del aparato cuando efectúe un calentamiento.

¡PELIGRO! Descarga eléctrica debido a la penetración de líquidos.

 Apague el aparato y desenchúfelo de la alimentación eléctrica antes de empezar los

trabajos de limpieza o de desinfección.

 No deje que ningún líquido penetre en el interior de la carcasa.  No realice ninguna desinfección por pulverización.  Sólo vuelva a enchufar el aparato a la toma de corriente cuando este esté completamente

seco.

¡PELIGRO! Tensiones peligrosas para la vida en el interior del equipo.

 Asegúrese de que la carcasa esté cerrada y sin daños, de manera que no puedan tocarse

piezas del interior del dispositivo por descuido.

 No retire el revestimiento del aparato.  Sólo permita que personal técnico autorizado por Eppendorf abra el aparato.

¡PELIGRO! Descarga eléctrica.

 Apague el aparato y desenchúfelo de la toma de corriente antes de empezar a realizar

trabajos de mantenimiento y de limpieza.

¡ADVERTENCIA! Daños debidos a una alimentación de tensión incorrecta.

 Conecte el aparato únicamente a fuentes de tensión que cumplan los requisitos eléctricos

indicados en la placa de características.

 Sólo utilice tomas de corriente con conductor de puesta a tierra.

Instrucciones generales de seguridad

Mastercycler pro

Español (ES)

¡ADVERTENCIA! Peligro de pillarse o aplastarse los dedos.

 No meta los dedos entre tapa y carcasa al abrir o cerrar el aparato o al colocar la tapa en

posición de limpieza.

 No meta la mano debajo de la tapa abierta.  Cerciórese de que el soporte de la tapa haya encajado correctamente y que la tapa

permanezca en posición abierta.

¡ADVERTENCIA! Peligro de quemadura a causa del termobloque y de la tapa térmica caliente.

El termobloque, el lado interior de la tapa térmica y los tubos de reacción / placas de PCR alcanzan rápidamente temperaturas por encima de los 50 °C.

 Mantenga la tapa térmica cerrada hasta que la temperatura del termobloque sea inferior a

30 °C.

 Preste atención a los símbolos en el termobloque que advierten de la existencia de

superficies calientes.

 Tenga en cuenta que la temperatura en la parte interior de la tapa térmica puede ser

mucho más alta que la temperatura indicada al utilizar esterillas de cierre hechas de material termoaislante.

 Antes de empezar con los trabajos de limpieza, debe esperar hasta que el termobloque y la

tapa térmica se hayan enfriado.

¡ADVERTENCIA! Peligro de lesionarse con piezas afiladas.

Si el Mastercycler pro se encuentra en posición abierta, es posible tener contacto con piezas afiladas del aparato. Durante la limpieza del Mastercycler pro los bordes afilados del cable Rollflex y del carril guía pueden ocasionar lesiones.

 Trabaje con mucha prudencia y utilice guantes protectores.

¡ADVERTENCIA! Deterioro de la salud a causa de una aplicación equivocada.

En caso de un calentamiento mal efectuado, es posible que material de muestra se libere y el termobloque o la tapa térmica se dañen.

 Asegúrese de que las placas de PCR estén posicionadas correctamente dentro del

termobloque.

 Siempre caliente el termobloque y la tapa térmica al mismo tiempo. Si se efectúa el

calentamiento con tapa térmica fría o sin la tapa térmica, los envases de reacción pueden reventar.

¡ADVERTENCIA! Riesgo para la salud debido a la contaminación del aparato.

 Efectúe una descontaminación antes de que almacene o envíe el aparato y/o los

accesorios.

Instrucciones generales de seguridad

Mastercycler pro Español (ES)

¡ATENCIÓN! Liberación de material de muestra a causa de tubos de ensayo inapropiados.

Tubos de ensayo inapropiados pueden ser dañados de tal forma que material de muestra logre escaparse.

 Utilice exclusivamente tubos de ensayo, cuyas propiedades correspondan como mínimo a

los requisitos descritos en las presentes instrucciones de uso.

 Tenga esto especialmente en cuenta al trabajar con material infeccioso.

¡ADVERTENCIA! Deficiencias de seguridad por accesorios y piezas de recambio incorrectas.

La utilización de componentes y piezas de recambio no recomendadas por Eppendorf merma la seguridad, el funcionamiento y la precisión del aparato. En caso de daños que se hayan producido a casua de accesorios o piezas de recambio no recomendadas o a causa de un uso inadecuado, Eppendorf no asumirá ninguna clase de responsabilidad.

 Utilice únicamente accesorios y piezas de recambio originales recomendadas por

Eppendorf.

¡ADVERTENCIA! Peligro de lesionarse al levantar y transportar el aparato.

Una postura equivocada durante el intento de levantar el aparato puede provocar daños permanentes. La caída de un aparato puede ocasionar lesiones.

 No levante el aparato sujetándolo del asa o de la tapa térmica.  Cargue el aparato con ambas manos para que no pueda resbalar.  Siempre mantenga el tronco en posición erguida cuando levante y cargue el aparato.

¡ATENCIÓN! Deterioro de la tapa térmica a causa de tubos de ensayo u objetos afilados.

 Utilice exclusivamente tubos de ensayo, cuyas propiedades correspondan como mínimo a

los requisitos descritos en las presentes instrucciones de uso.

 No utilice objetos afilados o puntiagudos en la tapa térmica.  Preste especial atención de no dañar la tapa térmica al efectuar la limpieza.

¡ATENCIÓN! Deterioro del aparato a causa de derrame de líquido.

Si la unidad de presión flexible de la tapa térmica está dañada, es posible que se derrame líquido y penetre al interior del aparato.

1. Deje la tapa abierta.

2. Desenchufe de inmediato el enchufe de la alimentación eléctrica.

3. Póngase en contacto con el servicio técnico de Eppendorf.

¡ATENCIÓN! Deterioro del termobloque a causa de funcionamiento inapropiado.

 No vierta ningún material de muestra directamente en el termobloque.

Instrucciones generales de seguridad

Mastercycler pro

Español (ES)

¡ATENCIÓN! Deterioro de componentes electrónicos por formación de condensado.

Después del transporte del aparato de un entorno frío a un entorno más cálido, puede formarse condensado en el aparato.

 Después de haber colocado el aparato en su sitio, deje que el aparato se caliente durante

un período de por lo menos 12 horas antes de conectarlo a la alimentación eléctrica.

¡ATENCIÓN! Daños materiales debido a una superficie desigual.

 Coloque el aparato sobre una superficie plana y estable, que tenga suficiente capacidad de

carga. El aparato no debe ser colocado sobre un carro o una superficie inclinada.

¡ATENCIÓN! Daños por sobrecalentamiento.

 No coloque el aparato cerca de fuentes de calor (p. ej. calefacción, armarios de secado).  No exponga el aparato a la radiación solar directa.  Garantice que haya suficiente circulación de aire dejando un espacio de por lo menos

10 cm alrededor de todo el aparato hacia aparatos vecinos o hacia la pared y dejando la parte inferior del aparato libre.

¡ATENCIÓN! Seguridad insuficiente debido a la ausencia del manual de instrucciones.

 Incluya siempre el manual de instrucciones cuando entregue el aparato a otras personas.  En caso de pérdida del manual de instrucciones, solicite otro. La versión actual del manual

de instrucciones y las indicaciones de seguridad también las puede encontrar en nuestra página Web www.eppendorf.com

100

Instrucciones generales de seguridad

Mastercycler pro Español (ES)

2.5 Símbolos de advertencia en el aparato

En esta sección se explican los símbolos de advertencia Mastercycler pro.

1 ¡ADVERTENCIA!

Peligro de quemadura a causa del termobloque (triángulo con la punta hacia abajo) o a causa de la parte interior caliente de la tapa calefactora (triángulo con la punta hacia arriba). En caso de funcionamiento con gradientes, la temperatura en el lado derecho es superior a la del lado izquierdo.

2 ¡ADVERTENCIA!

Peligro de quemaduras provocadas por superficies calientes.

Eppendorf Mastercycler pro User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

English (EN)

1 Operating instructions

1.1 Using this manual

1.2 Danger symbols and danger levels

1.3 Symbols used

1.4 Abbreviations used

2 Product description

2.1 Main illustration

2.2 Delivery package

2.2.1 Mastercycler pro and Mastercycler pro S

2.2.2 Mastercycler pro 384

2.3 Features

2.3.1 Features of the Mastercycler pro variants

2.3.2 Networking

3Safety

3.1 Intended use

3.2 User profile

3.3 Note on product liability

3.4 Warnings for intended use

3.5 Warning signs on the device

4 Installation

4.1 Unpacking the device

4.2 Selecting the location

4.2.1 Space requirements and bearing capacity

4.2.2 Mains connection

4.3 Installing the instrument

4.3.1 Operating a Mastercycler pro via a control panel

4.3.2 Operating multiple Mastercycler pro via a control panel ("mini-satellite" system)

4.3.3 Connecting Mastercycler pro to an existing Mastercycler ep

4.3.4 Using the USB ports

4.3.5 Connecting the printer

4.3.6 Connecting the mouse

4.4 Shut down

5 Operation

5.1 Initial steps

5.1.1 Using the vapo.protect heated lid

5.1.2 Control panel keys

5.2 Initial start-up of the Mastercycler pro

5.2.1 Defining the administrator PIN

5.2.2 Other steps

5.3 User login and logout

5.3.1 Logging in as a user

5.3.2 Changing the user

5.3.3 Logging out as a user

5.4 Overview of operation with the control panel

5.4.1 Navigation tree overview

5.4.2 Using the navigation tree

5.4.3 Using external storage media

5.4.4 Selecting export file formats

5.5 Managing folders and programs

5.5.1 Creating folders and programs

5.5.2 Copying folders and programs

5.5.3 Deleting folders and programs

6 Programming

6.1 Overview of the program editor

6.1.1 Opening the program editor

6.1.2 Structure of the program editor

6.2 Defining general settings

6.2.1 Opening the header

6.2.2 Editing header settings

6.3 Editing programs

6.3.1 Inserting a program step

6.3.2 Editing parameters

6.3.3 Deleting individual program steps

6.3.4 Deleting all program steps

6.3.5 Exporting a program listing

6.3.6 Saving a program and exiting the editor

7PCR run

7.1 Preparation

7.1.1 Selecting the sample tubes

7.1.2 Loading the thermoblock

7.1.3 Sample volume

7.2 Starting the program

7.2.1 Status view

7.3 Interrupting or stopping the program

7.3.1 Interrupting the program