Eppendorf Mastercycler pro User Manual
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Mastercycler pro
Operating manual
©
Copyright
permission of Eppendorf AG.
Trademarks
2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior
Eppendorf
registered trademarks of Eppendorf AG.
Registered trademarks are not marked with
Notice
The software of the Mastercycler pro contains open source software. License information is available as
PDF documents via the Eppendorf node. To export the license documents, connect a memory device, select
the Eppendorf node in the navigation tree and press the softkey.
®
, the eppendorf logo, Mastercycler®, SteadySlope®, vapoprotect® and Eppendorf twin.tec® are
®
or ™ in all cases in this manual.
6321 900.150-03/082013
Table of contents
Mastercycler pro
English (EN)
Operating manu al Mastercycler proseeon p.Fig.Tab.p.
English (EN)Operating manual
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2.1 Mastercycler pro and Mastercycler pro S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2.2 Mastercycler pro 384 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.3.1 Features of the Mastercycler pro variants. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.3.2 Networking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3
3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.3 Note on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.5 Warning signs on the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.1 Unpacking the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.2.1 Space requirements and bearing capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.2.2 Mains connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.3 Installing the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.4 Shut down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1 Initial steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1.1 Using the vapo.protect heated lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1.2 Control panel keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.2 Initial start-up of the Mastercycler pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.2.1 Defining the administrator PIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.2.2 Other steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.3 User login and logout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.1 Logging in as a user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.2 Changing the user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.3 Logging out as a user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.4 Overview of operation with the control panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.4.1 Navigation tree overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.4.2 Using the navigation tree . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.4.3 Using external storage media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.4.4 Selecting export file formats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
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Mastercycler pro
4
English (EN)
5.5 Managing folders and programs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
5.5.1 Creating folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
5.5.2 Copying folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
5.5.3 Deleting folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
6 Programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.1 Overview of the program editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.1.1 Opening the program editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.1.2 Structure of the program editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.2 Defining general settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.2.1 Opening the header. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.2.2 Editing header settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.3 Editing programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
6.3.1 Inserting a program step. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
6.3.2 Editing parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
6.3.3 Deleting individual program steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
6.3.4 Deleting all program steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
6.3.5 Exporting a program listing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
6.3.6 Saving a program and exiting the editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7 PCR run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7.1 Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7.1.1 Selecting the sample tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7.1.2 Loading the thermoblock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.1.3 Sample volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.2 Starting the program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
7.2.1 Status view . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
7.3 Interrupting or stopping the program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.3.1 Interrupting the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.3.2 Continuing the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.3.3 Stopping the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.4 Using protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.5 Shutting down and switching off the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
8 System management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.1 Administrator functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.2 Managing user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.2.1 Creating user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.2.2 Editing user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
8.2.3 Deleting user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
8.2.4 Changing the PIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
8.3 System settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
8.4 Cycler functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
8.4.1 Cycler system functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
8.4.2 Self test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
8.5 Managing and backing up data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
8.5.1 Backing up data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
8.5.2 Exporting and importing programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
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English (EN)
8.6 Performing a control panel software update. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
8.6.1 Performing an automatic software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
8.6.2 Performing a manual software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
8.7 Transferring programs from the Mastercycler 533x. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
9 Quick start. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
9.1 Login . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
9.2 Creating folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
9.3 Copying folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
9.4 Deleting folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
9.5 Editing programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
9.6 Stopping and starting programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
9.7 Switching off the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.1.1 Cleaning the housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.1.2 Cleaning the heated lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.2 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
10.3 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
5
11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
11.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
11.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
12 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
12.1 Packing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
12.2 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
12.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
13 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
13.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
13.2 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
13.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
13.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
13.5 Control panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
14 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
14.1 Mastercycler pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
14.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
14.3 Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
14.4 Recommended auxiliary materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Table of contents
Mastercycler pro
6
English (EN)
Operating instructions
Mastercycler pro
English (EN)
1 Operating instructions
1.1 Using this manual
Read the operating manual before you operate the device for the first time.
Please view this manual as part of the product and keep it somewhere easily accessible.
If this manual is lost, please request another one. The current version of the operating manual can be
found on our website at www.eppendorf.com
The sequences of actions in this operating manual are described for operation using the
control panel and the language setting English.
Alternatively, you have the following possibilities:
• Operate the Mastercycler pro via a mouse (see p. 26).
• Change the dialog language (see p. 58).
.
1.2 Danger symbols and danger levels
7
Representation Meaning
DANGER
Risk of electric shock with potential for severe injury or death as a consequence.
DANGER
Risk of explosion with potential for severe injury or death as a consequence.
DANGER
Bio hazard with potential for risk to health or death as a consequence.
DANGER
Risk of burns.
WAR N ING
Warning of potential injury or health risk.
CAUTION
Refers to risk of damage to property.
Refers to particularly useful information and tips.
Operating instructions
Mastercycler pro
8
English (EN)
1.3 Symbols used
Depiction Meaning
1.
2.
Actions without a specified order
• List
Text Display text or software text
1.4 Abbreviations used
ESP
Electronic Sample Protection
Actions in the specified order
Additional information
PCL
Printer Command Language (standard for controlling printers)
PCR
Polymerase Chain Reaction
PDF
Portable Document Format
PIN
Personal Identification Number
PS
PostScript® (standard for controlling printers)
TSP
Thermal Sample Protection
USB
Universal Serial Bus
2 Product description
5
6
7
8
9
10
1 2 3 4
11
2.1 Main illustration
Abb. 2-1: Mastercyclerpro - front and rear view
Product description
Mastercycler pro
English (EN)
9
Fig. 2-1: Mastercycler pro - front and rear view
1Lid handle
For opening and closing and also locking and
unlocking the heated lid.
2 vapo.protect heated lid
3 Address display
4 Status lamp
5 Rear plate with ventilation slots
6 Mains connection socket
7 Mains switch
0 = switched off, I = switched on.
8Terminal block
See detail drawing (Fig. 2-2 on p. 10).
9 Retaining clip
For control panel.
10 Thermoblock
11 Nameplate
10
Printer RS 232 Control Panel CAN_out CAN_in Term Address
1 2 3 4 5 6 7
1
2
3
4
Product description
Mastercycler pro
English (EN)
Abb. 2-2: Terminal block
Fig. 2-2: Terminal block
1 Parallel interface
Connection for printer.
2 Serial interface (RS 232)
Connection for Eppendorf temperature validation
system.
3 Control panel connection
4CAN out
Data outlet for connecting several
Mastercycler pro or Mastercycler ep.
Abb. 2-3: Control panel
5CAN in
Data inlet for connecting several
Mastercycler pro or Mastercycler ep.
6Term switch
Termination of CAN bus when networking several
Mastercycler pro or Mastercycler ep.
Lower position: ON. Upper position: OFF.
7 Address dial
Setting of device address when networking
several Mastercycler pro or Mastercycler ep.
Fig. 2-3: Control panel
1Display
2USB port1
For mouse, printer or storage medium.
3USB port2
For mouse, printer or storage medium.
4 MMC slot
For data exchange via a MultiMediaCard (MMC).
Only devices meeting the IEC 950/EN 60950 (UL 1950) standards may be connected to the interfaces of the
Mastercycler pro and the control panel.
2.2 Delivery package
The contents of the delivery package depend on the variant of your Mastercycler pro.
2.2.1 Mastercycler pro and Mastercycler pro S
Product description
Mastercycler pro
English (EN)
11
QuantityOrder no.
(International)
1 6321 000.019 - 230 V / 50 - 60 Hz
or 6321 000.027 950030010 120 V / 50 Hz, with US- plug
1 6325 000.013 - 230 V / 50 - 60 Hz
or 6325 000.021 950030020 120 V / 50 Hz, with US-plug
1 6321 900.150 -
1-
1- -
1 - 100 pieces
1 - PCR clean, 2 pieces
1 - self-adhesive, 5 pieces
Order no.
(North America)
Description
Mastercycler pro
Mastercycler pro S
Operating Manual Mastercycler pro
Certificate of Conformity
Mains cable
Eppendorf PCR tubes 0.2 mL
Eppendorf twin.tec skirted
96-well PCR plates
PCR film
2.2.2 Mastercycler pro 384
QuantityOrder no.
(International)
1 6324 000.010 - 230 V / 50 - 60 Hz
or 6324 000.028 950030030 120 V / 50 Hz, with US-plug
1-
1-
1- -
1- 2 pieces
1 - self-adhesive, 5 pieces
Order no.
(North America)
Description
Mastercycler pro 384
Operating manual
Certificate of Conformity
Mains cable
Eppendorf twin.tec
384-well PCR plates
PCR film
12
Product description
Mastercycler pro
English (EN)
2.3 Features
Product variants
The Mastercycler pro is available in three variants.
• Mastercycler pro
• Mastercycler pro S
• Mastercycler pro 384
All variants are provided with the following features:
Triple Circuit Technology
All Mastercycler pro thermomodules feature extremely fast heating and cooling rates. Triple Circuit
Technology ensures a homogeneous temperature distribution and enables the generation of specific
temperature gradients for PCR optimizations.
SteadySlope
SteadySlope Technology ensures that the heating and cooling ramp rates for thermal modules are identical
in both gradient and normal operation. That means you can expect identical temperature control
characteristics in both optimization and routine experiments, as well as a reliable transfer of the
optimization results to the routine application.
vapo.protect
All variants of the Mastercycler pro have avapo.protect heated lid. A flexible pressure element ensures that
force is applied evenly to the surface of the tubes or plate. This reduces evaporation from the reaction
sample effectively. The vapo.protect heated lid performs automatic tube height adjustment for all sample
tubes and PCR plates.
TSP
The heated lid of the Mastercycler pro series is equipped with TSP technology (Thermal Sample
Protection). The thermoblock is continuously kept at 20°C during the lid heating phase. This means TSP
reduces the thermal load on your samples and minimizes the probability of non-specific product formation
during the PCR.
Self-test function
You can use the integrated self test function of the Mastercycler pro in combination with the control panel
with USB ports to check whether the thermoblock is functioning properly without the need for any further
tools. A separately available USB key is required for the self test function (see Accessories on p. 85).
Control
The Mastercycler pro is controlled via the control panel. It can also be controlled via the PC software
CycleManager pro.
Product description
Mastercycler pro
2.3.1 Features of the Mastercycler pro variants
Variant Features
Mastercycler pro • Freely programmable temperature gradient over 12 rows.
• A gradient range of up to 20°C.
•Robust, specially coated aluminum block.
• Holds up to 96 PCR tubes (0.2 ml) or a 96-well PCR plate.
Mastercycler pro S • Highest temperature control speed due to silver block: up to 6°C/s.
• Standard PCR possible in under 30 min.
• Pulse function for device-driven hot start for avoiding non-specific PCR product
formation.
• Freely programmable temperature gradient over 12 rows.
• Extended gradient range of up to 24°C.
• Holds up to 96 PCR tubes (0.2 ml) or a 96-well PCR plate.
Mastercycler pro 384 • Freely programmable temperature gradient over 24 rows.
• A gradient range of up to 20°C.
• Holds a 384-well PCR plate.
13
English (EN)
2.3.2 Networking
Mini-satellite system
Up to five devices from the Mastercycler pro or Mastercycler ep series can be interconnected and operated
via a central control panel. The procedure for setting up this type of configuration is described separately
(see Operating multiple Mastercycler pro via a control panel ("mini-satellite" system) on p. 23).
PC control
You can use the PC software CycleManager pro to control up to 30 devices from the Mastercycler pro or
Mastercycler ep series in a network. This software is available separately for purchase (see Accessories on
p. 85).
14
Product description
Mastercycler pro
English (EN)
Safety
Mastercycler pro
English (EN)
3Safety
3.1 Intended use
The Mastercycler pro is intended to be used in research and routine laboratories in the field of biomedical
sciences.
The Mastercycler pro is used to control the temperature of aqueous solutions, suspensions, and emulsions
in closed micro test tubes for enzymatic reactions, typically for polymerase chain reaction (PCR).
The Mastercycler pro is intended exclusively for indoor use.
3.2 User profile
This device must only be used by skilled personnel with the appropriate training.
15
Before using the device, read the operating manual carefully and familiarize yourself with the device's
mode of operation.
3.3 Note on product liability
In the following cases the protection provided in the device may be impaired. The liability for the function
of the device passes to the operator if:
• The device is not used in accordance with the operating manual.
• The device is used outside the range of application described herein.
• The device is used with accessories or consumables (e.g. tubes and plates) which are not recommended
by Eppendorf.
• The device is maintained or repaired by persons not authorized by Eppendorf.
• The owner has made unauthorized modifications to the device.
3.4 Warnings for intended use
Read the operating manual first and follow the general safety instructions below before using the
Mastercycler pro.
DANGER! Risk of explosion!
Do not operate the device in rooms where work is being carried out with explosive
substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which could create an explosive
atmosphere.
16
Safety
Mastercycler pro
English (EN)
DANGER! Risk when handling toxic or radioactively-marked liquids or pathogenic germs.
Follow national regulations governing the handling of these substances.
For complete instructions regarding the handling of germs or biological material of risk
group II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World
Health Organization, current edition of the Laboratory Biosafety Manual).
WARNING! Risk to health and bio hazard when operated with lid open.
The tube lids may pop open at high block temperatures.
Close the lid of the device when heating samples.
DANGER! Electric shock as a result of penetration of liquid.
Switch off the device and disconnect it from the power supply before starting cleaning or
disinfecting.
Do not allow any liquids to enter the inside of the housing.
Do not perform spay disinfection.
Only reconnect the device to the power supply once it is completely dry.
DANGER! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside the device
can be contacted by accident.
Do not remove the housing of the device.
Do not allow the device to be opened by anyone except service personnel who have been
specifically authorized by Eppendorf.
DANGER! Electric shock.
Switch off the device and disconnect the power plug before starting to maintain or clean.
WARNING! Damage due to incorrect power supply.
Only connect the device to power sources that match the electrical specifications on the
device's nameplate.
Use only sockets with protective earth.
WARNING! Injury from trapping or crushing.
Do not place your fingers between the lid and the housing when opening and closing the
device or when opening it into the cleaning position.
Do not reach in under the open lid.
Take care that the stand is properly engaged so that the lid stays in the opened position.
Safety
Mastercycler pro
English (EN)
WARNING! Burns from hot thermoblock and heated lid.
The thermoblock, the inner side of the heated lid and micro test tubes/PCR plates can reach
temperatures of over 50 °C very quickly.
The heated lid should be kept closed until temperatures of 30 °C or less are reached.
Take note of the symbols on the thermoblock warning that surfaces may be hot.
If you are using well caps consisting of thermally insulating material, please keep in mind
that the temperature on the inside of the heated lid can be significantly higher than the
temperature displayed.
Before starting cleaning wait for the thermoblock and heated lid to cool down.
WARNING! Injury from sharp-edged components.
When the Mastercycler pro is in the open position, sharp-edged components are exposed.
When cleaning the Mastercycler pro, the sharp edges on the rollflex cable and the guide rail
may cause injuries.
Take special care and wear protective gloves.
17
WARNING! Risk to health if used incorrectly.
If incubation is performed incorrectly, sample material may be released and the thermoblock
and the heated lid damaged.
Ensure that the PCR plates are correctly inserted in the thermoblock.
Always close the heated lid before heating the samples to prevent the lids of the tubes from
bursting open.
WARNING! Risk to health from contaminated device
Perform decontamination before storing or dispatching the device and/or its accessories.
CAUTION! Unsuitable tubes can result in sample material escaping.
Unsuitable tubes may be so severely damaged that sample material is released.
Use only tubes whose properties meet at least the requirements specified in these
operating instructions.
This is especially important when working with infectious material.
WARNING! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, function and precision of the device. Eppendorf accepts no warranty or
liability for damage caused by non-recommended accessories and spare parts or incorrect
use.
Use only accessories recommended by Eppendorf and original spare parts.
18
Safety
Mastercycler pro
English (EN)
WARNING! Risk of injury when lifting and transporting the device.
Lifting the device with the wrong posture may cause serious damage to your health. If the
device falls to the ground it can cause injuries.
Do not lift the device by the handle or the housing of the heated lid.
Carry the device securely with both hands to prevent it from slipping.
Always keep a straight back when lifting and carrying the device.
CAUTION! Damage to heated lid from sharp-edged sample tubes or objects.
Use only tubes whose properties meet at least the requirements specified in these
operating instructions.
Do not use any sharp-edged or sharp objects on the heated lid.
Extra caution should be exercised when cleaning so as not to damage the heated lid.
CAUTION! Escaping liquid can cause damage to the device.
If the flexible pressure unit of the heated lid is damaged, liquid can escape and enter the
device.
1. Leave the lid open.
2. Disconnect the power plug immediately from the power supply.
3. Contact Eppendorf Service.
CAUTION! Damage to the thermoblock from incorrect operation.
Do not fill sample material directly into the thermoblock.
CAUTION! Damage to electronic components through formation of condensation.
After the device has been moved from a cool to a warmer environment, formation of
condensation can occur inside the device.
Allow the device to warm up for at least 12 hours after being set up before you connect it
to the mains power supply.
CAUTION! Material damage from uneven work surface.
Position the device on level and stable work surface which is capable of bearing the weight
of the device. The device must not be placed on a trolley or at an angle.
CAUTION! Damage due to overheating.
Do not place the device close to sources of heat (e.g. radiator, drying cabinet).
Do not expose the device to direct sunlight.
Allow air to circulate freely by leaving at least 10 cm to adjoining devices or to the wall and
keep the underside of the device clear.
CAUTION! Lack of safety due to missing operating manual.
1
2
When passing on the device, always enclose the operating manual.
If you lose the operating manual, request a replacement. The current version of the
operating manual and the safety instructions can also be found on our
websitewww.eppendorf.com
.
3.5 Warning signs on the device
This section explains the warning signs used on the Mastercycler pro.
Safety
Mastercycler pro
English (EN)
19
1 WARNING
Risk of burns from the hot thermoblock (triangle with tip
facing down) or the hot inner side of the heated lid
(triangle with tip facing up).
Gradient operation can result in the temperature being
hotter on the right side than on the left side
2 WARNING
Risk of burns from hot surfaces.
20
Safety
Mastercycler pro
English (EN)
Installation
Mastercycler pro
English (EN)
4 Installation
4.1 Unpacking the device
WARNING! Risk of injury when lifting and transporting the device.
Lifting the device with the wrong posture may cause serious damage to your health. If the
device falls to the ground it can cause injuries.
Do not lift the device by the handle or the housing of the heated lid.
Carry the device securely with both hands to prevent it from slipping.
Always keep a straight back when lifting and carrying the device.
1. Release the four plastic locking clips at the long sides of the carton by pulling out the flaps.
2. Lift the carton lid and take the foam upper part off the Mastercycler pro.
3. Take the carton insert out of the carton lid (contains the mains cable).
4. Take the Mastercycler pro out of the plastic bag.
5. With both hands, hold the Mastercycler pro by the lower half of the housing and lift it out of the
package.
6. Remove all the packing material.
7. Visually check the device to ensure that no damage has occurred during shipment.
8. Check the completeness of the delivery (see Delivery package on p. 11).
21
Never use a device if it shows any signs of damage. In this case, contact your distributor or the
appropriate Eppendorf office.
• Retain the packing material for shipping or storing the Mastercycler pro at a later date.
• Ship the Mastercycler pro only in the original packing material.
22
Installation
Mastercycler pro
English (EN)
4.2 Selecting the location
4.2.1 Space requirements and bearing capacity
CAUTION! Material damage from uneven work surface.
Position the device on level and stable work surface which is capable of bearing the weight
of the device. The device must not be placed on a trolley or at an angle.
CAUTION! Damage due to overheating.
Do not place the device close to sources of heat (e.g. radiator, drying cabinet).
Do not expose the device to direct sunlight.
Allow air to circulate freely by leaving at least 10 cm to adjoining devices or to the wall and
keep the underside of the device clear.
Information regarding the dimensions and weight of the device components can be found separately(see
Weight/dimensions on p. 83).
When selecting a location for the Mastercycler pro ensure that the following criteria are met:
• The surface must have sufficient bearing capacity and ensure the required stability.
• The feet at the front of the unit must be secured to prevent them from slipping.
• The device must be positioned at a height which provides for convenient and safe operation.
• Additional space is required for free air circulation at the back of the device (10 cm) and for the control
panel at the front (14 cm).
4.2.2 Mains connection
CAUTION! Connected devices may be shut down due to a power supply overload.
The total current consumption of the devices can exceed the maximum permitted supply
rating when multiple power sockets are used. In this case, the mains fuse interrupts the
electric circuit. The connected devices are shut down.
Do not use multiple sockets when connecting several Mastercycler pro.
Information on the power consumption of the Mastercycler pro is provided separately (see Power supply on
p. 83).
• You need an earthed wall socket for the power supply of the Mastercycler pro. Use the supplied power
cable to connect the Mastercycler pro.
• The control panel is supplied via the Mastercycler pro and does not require an additional power supply.
Installation
Term
Mastercycler pro
English (EN)
4.3 Installing the instrument
This section describes how to start up the Mastercycler pro with the control panel and how to operate
several devices belonging to the Mastercycler pro and Mastercycler ep series with each other in a network.
The connections and switches are located at the back of the device (see Main illustration on
p. 9).
• Follow the steps below to define the settings and connect the devices before switching on
the Mastercycler pro.
• Never disconnect the control panel from a Mastercycler pro that is switched on.
4.3.1 Operating a Mastercycler pro via a control panel
1. Connect the cable on the control panel to the Control Panel connector of the
Mastercycler pro.
23
2. Set Term switch to ON (lower position).
3. Use the address dials to set the address of the Mastercycler pro to 01. To do this,
turn the left dial into position 0 and the right dial to 1, as shown in the illustration.
4. Connect the power cable to the Mastercycler pro and to the mains supply.
4.3.2 Operating multiple Mastercycler pro via a control panel ("mini-satellite" system)
CAUTION! Connected devices may be shut down due to a power supply overload.
The total current consumption of the devices can exceed the maximum permitted supply
rating when multiple power sockets are used. In this case, the mains fuse interrupts the
electric circuit. The connected devices are shut down.
Do not use multiple sockets when connecting several Mastercycler pro.
A control panel can be used to control up to five devices belonging to the Mastercycler pro or Mastercycler
ep series (also in combination). Each additional device connected requires its own CAN bus cable. CAN bus
cables are available separately for purchase (see Accessories on p. 85).
24
CAN_out
CAN_in
Term
Term
CAN_out Control PanelCAN_in Term Address CAN_out CAN_in Term AddressCAN_out CAN_in Term Address
Installation
Mastercycler pro
English (EN)
1. Connect the cable on the control panel to the "Control Panel" connector of the first
device.
2. Connect the CAN bus cable to the "CAN_out" connector of the first device.
3. Connect the free end of the CAN bus cable to the "CAN_in" connector of the
second device.
Connecting additional devices
4. Always use the CAN bus cable to connect the "CAN_out" connector of the last
connected device to the "CAN_in" connector of the new device.
Device 3 Device 2 Device 1
Abb. 4-1: Address and Term settings and wiring connections for a network consisting of t hree devices
5. Set the Term switch of the first and last device to "ON" (lower position).
6. Set the Term switch of all other devices to "OFF" (upper position).
Note: If the network consists of exactly two devices, the Term switch of both
devices must be set to "ON".
7. For the first device connected to the control panel, set the address to 01.
8. Define one unique address from 02 to 05 on each additional device.
Fig. 4-1: Address and Term settings and wiring connections for a network consisting of three devices
Each address may only be assigned once within the network.
The address that was set for the Mastercycler pro will be displayed by the two-digit address
display 3 on the heated lid during operation (see Main illustration on p. 9).
Installation
Mastercycler pro
English (EN)
4.3.3 Connecting Mastercycler pro to an existing Mastercycler ep
You can extend an existing system of one or more Mastercycler ep devices with a corresponding control
panel using devices of the Mastercycler pro series.
When operating the Mastercycler pro with a Mastercycler ep control panel (without USB
connections), the following restrictions apply:
• The self test function is not available.
• The PDF file format is not available for exporting programs, protocols and log files.
• No USB devices can be connected. Information for connecting a PS/2 mouse can be found
in the Mastercycler ep operating manual.
Prerequisites
Before a Mastercycler pro can be connected to an existing Mastercycler ep system, the software of the
control panel and of the connected Mastercycler ep has to be updated. For more information, contact the
Eppendorf branch for your country (see back cover flap).
25
1. Follow the instructions in the Mastercycler ep operating manual to update the control panel software.
2. After restarting the control panel, synchronize all connected Mastercycler ep with the control panel.
3. Shut down the control panel (see p. 54) and switch off all Mastercycler ep devices.
4. Set the address of the new Mastercycler pro to the next free address number.
Example:
If you already operate two Mastercycler ep devices, set the address of the new
Mastercycler pro to 03.
5. Connect the cables and define the Term settings.
6. Switch all devices on and register them at the control panel.
The Mastercycler pro is displayed as an additional cycler node on the control panel.
4.3.4 Using the USB ports
The control panel of the Mastercycler pro has two USB ports (see Fig. 2-3 on p. 10) for connecting a
mouse, printer, storage medium or the self test USB key.
If you would like to use more than two USB devices at the control panel at the same time, you can make
use of a USB hub.
26
Installation
Mastercycler pro
English (EN)
4.3.5 Connecting the printer
The Mastercycler pro can be connected to a parallel interface printer in order to print protocols and
programs in text format.
Connect the printer via a parallel data cable to the parallel interface (see Fig. 2-2 on p. 10) of the
Mastercycler pro.
A printer with USB interface can be connected to the control panel to print protocols, programs and self
test certificates.
Prerequisites
®
The USB printer must be compatible with the PostScript
can be found in the operating manual for your printer.
1. Us a USB cable to connect the printer to one of the two USB ports (see Fig. 2-3 on p. 10) of the control
panel.
or PCL printer standards. Further information
4.3.6 Connecting the mouse
You can connect a USB mouse (not included in the delivery package) to the control panel.
Connect the plug of the mouse to one of the USB ports on the right-hand side of the control panel (see
Fig. 2-3 on p. 10).
The mouse pointer appears in the display.
4.4 Shut down
Proceed as follows if you do not want to use the Mastercycler pro for longer than a week.
1. Load the block with an empty PCR plate(see p. 50).
2. Close the heated lid and flip the handle forwards (see p. 27).
3. Disconnect the Mastercycler pro from the power supply.
Do not leave the lid of the Mastercycler pro in an open position in order to avoid the block from becoming
contaminated.
Operation
Mastercycler pro
English (EN)
5 Operation
5.1 Initial steps
Check the correct installation
Before the Mastercycler pro is started up for the first time, ensure the following:
• The device is correctly connected.
• The device is free of damage.
• Make sure that air can circulate freely around the ventilation slots on the rear of the device and around
the fan behind the feet.
5.1.1 Using the vapo.protect heated lid
Thevapo.protectheated lid features a heated flexible pressure unit. When the lid closes, the pressure unit
comes to rest lightly on the surface of the sample tubes. A liquid cushion in the pressure unit is then used to
create a pressure to ensure that the tubes are pressed uniformly into the thermoblock thereby protecting
the tube seals against evaporation. The heating process prevents condensation from forming in the upper
area of the tube when the reaction liquid is heated.
27
5.1.1.1 Closing the heated lid
Prerequisites
The thermoblock of the Mastercycler pro is loaded with sample tubes or a PCR plate (see Loading the
thermoblock on p. 50).
CAUTION! Damage to heated lid from sharp-edged sample tubes or objects.
Use only tubes whose properties meet at least the requirements specified in these
operating instructions.
Do not use any sharp-edged or sharp objects on the heated lid.
Extra caution should be exercised when cleaning so as not to damage the heated lid.
28
2
1
Operation
Mastercycler pro
English (EN)
1. Pull vapo.protect heated lid forwards at the lid handle as far as possible so that the lid handle can be
rotated.
2. Flip the handle over the heated lid until it clicks into place.
You can now start a program run or manually temperature-control the lid and the samples.
5.1.1.2 Opening the heated lid
WARNING! Risk to health and bio hazard when operated with lid open.
The tube lids may pop open at high block temperatures.
Close the lid of the device when heating samples.
WARNING! Risk of burns from the hot thermoblock and heated lid.
The thermoblock and the heated lid very quickly reach temperatures above 95 °C.
Keep heated lid closed until the temperature of the thermoblock is below 30 °C.
Open the heated lid in one go as follows:
Flip the lid handle up over the heated lid and push the heated lid by the handle to the rear position until
it stops.
Operation
Mastercycler pro
English (EN)
5.1.1.3 Status lamp
The vapo.protect heated lid is equipped with a status lamp 2(see Main illustration on p. 9) which allows the
current operating status of the Mastercycler pro to be visible from far off.
Status lamp Operating status
Illuminates green The Mastercycler pro is idle.
Flashes green A program run is active.
Flashes orange The program run is waiting for user intervention in a pause or hold step(see
Editing programs on p. 44).
Flashes red A fault has occurred. Additional information on the type of fault is displayed on
the control panel (see Error messages on p. 77).
5.1.1.4 Address display
29
The vapo.protect heated lid is equipped with a two-digit address display so that the cycler can be identified
easily (see Main illustration on p. 9).
The displayed address number matches the cycler numbering in the operating software. The address
setting is described separately.
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