Read this operating manual completely before using the device for the first time. Also
observe the instructions for use of the accessories.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
You will find the current version of the operating manual for all available languages on
our webpage under www.eppendorf.com
1.2Danger symbols and danger levels
The safety instructions of this operating manual indicate the following danger symbols
and danger levels:
1.2.1Danger symbols
Electric shockHazard point
CutsMaterial damage
Biohazard
.
7
1.2.2Danger levels
DANGERWill lead to severe injuries or death.
WAR NIN GMay lead to severe injuries or death.
CAUTIONMay lead to light to moderate injuries.
NOTICEMay lead to material damage.
1.3Symbols used
Depiction Meaning
1.
2.
Actions in the specified order
Actions without a specified order
• List
Text Display text or software text
Direction of movement
Additional information
Product description
FemtoJet
8
®
4i
English (EN)
2Product description
2.1Delivery package
NumberDescription
1FemtoJet 4i
1Mains/power cord
1Injection tube
1Operating manual
2.1.1Delivery package – Accessories
NumberDescription
1Universal capillary holder
1Grip head 0 for capillary (outer diameter 1.0 mm to 1.1 mm)
1Adapter for Femtotips
1Foot control
2.2Features
The Microinjector can be used to inject very small amounts of fluid into cells. The
parameters for pressure and time are set on the device and controlled by the software. It
is possible to trigger the injection on the Microinjector or on a connected
micromanipulator by Eppendorf. The semiautomatic injection movement is controlled by
the Microinjector or the micromanipulator. The required pressure is generated by a
soundproof internal compressor.
2.3Exemplary set-up of a microinjection system
FemtoJet
inject
prog 1
prog 2
count
clean
menu
enter
auto
man
2
1
3
6
5
4
Abb. 2-1:Microinjection system with FemtoJet 4i
Product description
FemtoJet
®
4i
English (EN)
9
Fig. 2-1:Microinjection system with FemtoJet 4i
1 Micromanipulator InjectMan 4
2 Universal capillary holder with
capillary
3Inverse microscope
4 Injection tube
5Control board InjectMan 4
6 Microinjector FemtoJet 4i
Product description
menu
enter
auto
man
inject
clean
count
prog 1
prog 2
FemtoJet
1
2
4
5763
FemtoJet
10
®
4i
English (EN)
2.4Main illustration
Abb. 2-2:Front and rear side
Fig. 2-2:Front and rear side
1Display
2 Control panel
3 Port for injection tube
4 Rotary knobs for injection parameters
5 Name plate
Lower side of device
6Interfaces
7Venting
Lower side of device
2.4.1Interfaces
MANIPULATOR
FOOT SWITCH
SERVICE
4
5
6
213
Abb. 2-3:Interfaces
Fig. 2-3:Interfaces
Product description
FemtoJet
®
4i
English (EN)
11
1 Mains/power connection
2Mains/power switch On/Off
3Micro fuse
4 Port for micromanipulator or
computer
5 Connection for foot or hand control
6 Service connection
Product description
1
2
3
4
5
6
7
8
10
9
FemtoJet
12
English (EN)
®
4i
2.5Name plate
Abb. 2-4:Name plate
Fig. 2-4:Name plate
1 Manufacturer
2Product number
3Voltage
4Micro fuse
5Frequency
6Labelings
7Address of manufacturer
8 Serial number
9Output
10 Current consumption
Product description
countcountcount
clean
prog 1
56
7
8
9
4
3
2
1
FemtoJet
English (EN)
2.6Control panel
The keys of the control panel are used to switch on the Microinjector, to perform
functions, to select programs and to navigate through the menu.
Abb. 2-5:Control panel
®
4i
13
Fig. 2-5:Control panel
1 standby key
Activate/deactivate standby mode
2 menu enter key
Open the menu
3Arrow key
Navigate the menu
4 auto man key
Toggle between automatic and manual
6 prog 2 key
Select or save parameter set 2
7 inject key
Perform an injection
8 clean key
Clean the capillary
9 count key
Set the counter to zero
injection
5 prog 1 key
Select or save parameter set 1
Product description
enter
FemtoJet
14
®
4i
English (EN)
2.7Rotary knobs
The rotary knobs are used to set the injection parameters as injection time, injection
pressure and compensation pressure.
Abb. 2-6:Rotary knobs
cleanclean
auto
man
Fig. 2-6:Rotary knobs
1 Rotary knob
Set the injection pressure p
2 Rotary knob
Set the injection time t
countcountcountcount
321
3Rotary knob
i
i
Set the compensation pressure p
c
2.8Foot control
You can connect the foot control to the Microinjector.
The foot control corresponds to the following key on the Microinjector:
• Foot control – inject key
Product description
FemtoJet
®
4i
English (EN)
15
Abb. 2-7:Foot control with plug
Fig. 2-7:Foot control with plug
2.9Hand control
The hand control is not included in the delivery package and must be ordered
separately.
You can connect the hand control to the Microinjector.
The hand controls correspond to the following keys on the Microinjector:
• Left hand control – inject key
• Right hand control – clean key
The inject and clean keys remain active when the hand control is connected.
2.10Pressure parameters
The parameters are used to define the pressure and time for the injection and the
cleaning of the capillary.
The pressure parameters include the following parameters:
The inject key remains active when the foot control is connected.
• Compensation pressure – p
• Injection pressure – p
• Injection time – t
i
c
i
• Operating pressure
• Rinsing pressure
Product description
3
4
5
1
2
FemtoJet
16
®
English (EN)
4i
2.10.1 Compensation pressure p
c
The compensation pressure prevents the liquid from rising from the Petri dish into the
capillary due to the capillary action. Due to the compensation pressure, some liquid will
leak constantly from the capillary tip. This prevents the injection material from clumping
together.
Abb. 2-8:Pressure ratios in the capillary
Fig. 2-8:Pressure ratios in the capillary
1Capillary
2 Liquid with injection material
3 Compensation pressure p
c
4Hydrostatic pressure
5 Capillary action
2.10.2 Injection pressure p
i
The injection pressure defines the pressure used for injecting liquid into the cell. During
the injection process, the injection pressure is applied as long as the injection time is
running. To inject liquid into a cell, the injection pressure must be higher than the inside
pressure of the cell.
2.10.3 Injection time t
i
The injection time defines the time period for injecting the liquid. The start of the
injection time depends on the presettings of the micromanipulator. The injection time
begins either when triggering the injection function or when reaching the lower safety
limit. The injection pressure is applied as long as the injection time is running.
2.10.4 Rinsing pressure
1234
67
5
The rinsing pressure is used to clean the capillary.
Product description
FemtoJet
®
4i
English (EN)
17
2.10.5 Operating pressure p
w
The operating pressure subsumes the injection pressure, compensation pressure and
rinsing pressure.
2.11Self-calibration
The Microinjector performs a calibration every two hours. During the calibration, the
ventilation valve opens and the collected condensation water is discharged.
2.12Universal capillary holder
You can insert a capillary or a Femtotip into the universal capillary holder.
Abb. 2-9:Universal capillary holder
Fig. 2-9:Universal capillary holder
1Capillary
2Grip head
3Knurled screw
5 Connection for injection tube
6Femtotips
7 Adapter for Femtotips
4 Universal capillary holder
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