Eppendorf Easypet 3 User Manual
Operating manual Easypet® 3
see
on p.Fig.Tab.p
.
Operating manual
4430 900.019
Easypet® 3
Operating manual
©
Copyright
the prior permission of the copyright owner.
eppendorf
2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without
®
and Easypet® are registered trademarks of Eppendorf AG, Hamburg, Germany.
Trademarks are not marked in all cases with ™or
®
in this manual.
4430 900.019-01/122012
Easypet® 3 — Operating manual
Table of contents
1 Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Hazard icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4 Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.3 Warranty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.4 Materials. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.5 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4.2 Mains/power supply device assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4.3 Removing the discharge protector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.4 Rechargeable battery status display during operation . . . . . . . . . . . . . . . . . . . . . . . . 13
4.4.1 The device is ready for operation.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.4.2 Charging the rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.4.3 Rechargeable battery charging. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.4.4 The rechargeable battery is charged. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.1 Loading the rechargeable battery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.2 Inserting the pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.3 Pipette types. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.4 Speed control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.5 Aspirating liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.6 Dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.6.1 Flow-out . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.6.2 Blow-out . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
1
Table of contents
3
Easypet® 3 — Operating manual
1
Table of contents
5.7 Using the wall mount . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.7.1 Mounting the wall mount . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.7.2 Removing the wall mount . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.8 Using the shelf stand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.1 Disassembling the pipette clamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.2.1 Cleaning the Pipette Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.2.2 Cleaning the pipette clamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.3 Replacing the rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.4 Mounting the pipette clamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.5 Checking the leak tightness . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
8.1 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
8.2 Mains/power supply device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
8.3 Rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
8.4 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
9 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
10.1 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
10.2 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
10.3 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
10.4 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
4
Easypet® 3 — Operating manual
1 Operating instructions
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time. Also
observe the instructions for use of the accessories.
This operating manual is part of the product. Thus, must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. The current version is available on our
website: www.eppendorf.com
1.2 Danger symbols and danger levels
1.2.1 Hazard icons
Biohazard Explosion
Cuts Toxic substances
Hazard point Material damage
1.2.2 Degrees of danger
The safety instructions in this operating manual indicate the following degrees of danger:
.
1
Operating instructions
DANGER Will lead to severe injuries or death.
WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
5
Easypet® 3 — Operating manual
1.3 Symbols used
Depiction Meaning
1
1.4 Glossary
Elapse time Time required to empty a pipette from the upper part of the scale to the lowest
Operating instructions
You are requested to perform an action.
1.
2.
Perform these actions in the sequence described.
• List
References useful information.
E
scale graduation or to fully empty a measuring pipette or volumetric pipette.
6
Easypet® 3 — Operating manual
Easypet 3
1
2
3
4
5
6
7
8
9
10
11
12
13
2 Product description
2 Product description
2.1 Main illustration
Abb. 1:Easypet3 with accessories
2
Product description
Fig. 1: Easypet3 with accessories
1 Shelf stand 2 Rechargeable battery status display
3 Aspirating button 4 Dispensing button
5 Rechargeable battery compartment lid 6 Connector socket
7 Aspirating cone 8 Pipette adapter
9 Membrane filter 10 Seal for filter adapter
11 Filter adapter 12 Pressure compensation opening
13 Pipette clamp
7
Easypet® 3 — Operating manual
2.2 Delivery package
Quantity Description
1 Easypet 3
1 Lithium polymer battery
2 Non-sterile membrane filter, 0.45μm
2
1Wall mount
1 Shelf stand (not available in the USA)
1 Universal power supply
1 Operating manual
1 Short operating manual
1CD
2.3 Warranty
For warranty claims, please contact your local Eppendorf sales partner. If the housing of the
Product description
pipette controller is opened by unauthorized individuals, or the device is misused, no warranty
claim may be made. The rechargeable battery and all other wear parts are excluded from the
warranty.
8
Easypet®
— Operating manual
2.4 Materials
NOTICE! Aggressive substances may damage the Easypet3 and accessories.
Check the chemical resistance before using organic solvents or aggressive
NOTICE!
The pipette controller assemblies are composed of the following materials:
Component Material
Housing, aspirating cone, aspiration button,
dispensing button, membrane filter housing,
wall holder, shelf stands
Filter adapter Polybutylene terephthalate (PBT)
Pipette adapter Silicone
Filter membrane Polytetrafluoroethylene(PTFE)
Seal for filter adapter Hydrated acrylonitrile butadiene rubber
Tubes and valves Polymer fluorine rubber (FKM), polybutylene
chemicals.
Observe the cleaning instructions.
Additional information on the chemical resistance can be found on the enclosed CD
or on our webpage: www.eppendorf.com
.
Polypropylene(PP)
(HNBR)
terephthalate (PBT), polyphenylene sulfide
(PPS), silicone
2
Product description
Battery status display Cyclic olefin copolymer (COC)
2.5 Features
The Easypet3 is a battery-driven pipette controller. You can use glass or plastic pipettes in a
volume range of 0.1mL to 100mL.
A pump generates underpressure or overpressure to aspirate or dispense the liquid. The liquid
can also be dispensed solely via the atmospheric pressure.
The aspirating and dispensing speed is controlled by how far the control buttons are pressed in.
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Easypet® 3 — Operating manual
3 Safety
3Safety
3.1 Intended use
The pipette controller is intended for dispensing liquids. In-vivo applications (in or on the human
body) are not allowed.
The pipette controller may only be operated by skilled personnel who have received the
appropriate training. All users must have read the operating manual carefully and must have
become familiar with the device's mode of operation.
3.2 Warnings for intended use
WARNING! Damage to health due to infectious liquids and pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the national
3
Safety
WARNING!
WARNING!
regulations, the biological security level of your laboratory, the material safety data
sheets, and the manufacturer's application notes.
Wear personal protective equipment.
Follow the instructions regarding hygiene, cleaning and decontamination.
For complete instructions regarding the handling of germs or biological material in
risk group II or higher, please refer to the "Laboratory Biosafety Manual" (source:
World Health Organization, current edition of the Laboratory Biosafety Manual).
WARNING! Risk of explosion from potentially explosive atmospheres and
potentially explosive substances.
The Easypet3 in potentially explosive atmospheres.
Do not operate the Easypet3 in rooms where work is conducted using potentially
explosive substances.
Do not dispense any explosive, highly flammable (flash point <21°C), extremely
flammable (flash point <0°C), or highly reactive substances with the Easypet3.
Do not use the Easypet3 to dispense any substances that could create an
explosive atmosphere.
10
WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.
Wear personal protective equipment.
WARNING!
Observe the national regulations for handling these substances.
Observe the material safety data sheets and manufacturer's application notes.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf
CAUTION!
may impair the safety, functioning and precision of the device. Eppendorf cannot be
held liable or accept any liability for damage resulting from the use of incorrect or
non-recommended accessories and spare parts, or from the improper use of such
equipment.
Only use accessories and original spare parts recommended by Eppendorf.
Easypet® 3 — Operating manual
CAUTION! Danger to people due to grossly negligent use.
Never direct the opening of a Easypet3 at yourself or other people.
CAUTION!
Only initiate dispensing if it is safe to do so.
For all dispensing tasks, make sure that you do not place yourself or other people at
risk.
NOTICE! Damage to device due to penetration of liquids.
Do not allow any liquids to penetrate the inside of the housing.
NOTICE!
If liquid has entered the inside of the housing, the inner parts may only be repaired
by Eppendorf AG service partners. Please contact your responsible sales office
before reshipping the parts.
NOTICE! Damage to device from missing pipette.
Use Easypet 3 only when the pipette is inserted.
NOTICE!
If you would like to dispense highly flammable or extremely flammable liquids, we
Hint!
recommend using a positive displacement system consisting of manual Multipettes/
Repeaters and Combitips. Check the chemical resistance and observe the safety notes
before using the Multipette/Repeater.
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised. Liability for
any resulting property damage or personal injury is then transferred to the operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
3
Safety
11
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