Eppendorf Easypet 3 User Manual

nual

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Register your instrument!

www.eppendorf.com/myeppendorf

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manual

Easypet

Operating manual

3

®

Copyright

©

2014 Eppendorf AG, Hamburg. All rights reserved, including graphics and
images. No part of this publication may be reproduced without the prior permission of the
copyright owner.

Eppendorf

®

and the Eppendorf logo are registered trademarks of Eppendorf AG,

Hamburg, Germany.

®

Easypet

Registered trademarks and protected trademarks are not marked in all cases with

is a registered trademark of Eppendorf AG, Hamburg, Germany.

®

or ™

in this manual.

4430 900.019-03/022014

Table of contents

Easypet

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English (EN)

Table of contents

1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.4 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2 RFID chip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.2.1 RFID position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.4 Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.5 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.6 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

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4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.2 Mains/power supply device assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.3 Removing the discharge protector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.4 Rechargeable battery status display during operation . . . . . . . . . . . . . . . . . 14

4.4.1 The device is ready for operation. . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.4.2 Charging the rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . 15

4.4.3 Rechargeable battery charging . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

4.4.4 The rechargeable battery is charged. . . . . . . . . . . . . . . . . . . . . . . 15

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5.1 Loading the rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5.2 Inserting the pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.3 Pipette types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.4 Speed control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.5 Aspirating liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.6 Dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5.6.1 Flow-out. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5.6.2 Blow-out . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Table o f con tents

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English (EN)

5.7 Using the wall mount . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.7.1 Mounting the wall mount . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.7.2 Removing the wall mount . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.8 Using the shelf stand. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.1 Disassembling the pipette clamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.2.1 Cleaning the pipetting aid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.2.2 Cleaning the pipette clamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

6.3 Preserving the battery capacity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

6.3.1 Longer periods without operation . . . . . . . . . . . . . . . . . . . . . . . . . 27

6.4 Replacing the rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

6.5 Mounting the pipette clamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

6.6 Checking the leak tightness . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

8 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

8.1 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

8.2 Mains/power supply device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

8.3 Rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

8.4 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

9 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

9.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

9.2 Serological pipettes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

10.1 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

10.2 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

10.3 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

10.4 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Operating instructions

English (EN)

Easypet

®

1 Operating instructions

1.1 Using this manual

 Read this operating manual completely before using the device for the first time. Also

observe the instructions for use of the accessories.

 This operating manual is part of the product. Thus, it must always be easily accessible.
 Enclose this operating manual when transferring the device to third parties.
 You will find the current version of the operating manual for all available languages on

our webpage under www.eppendorf.com

1.2 Danger symbols and danger levels

The safety instructions of this operating manual indicate the following danger symbols
and danger levels:

1.2.1 Danger symbols

Biohazard Explosion

Cuts Toxic substances

Hazard point Material damage

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1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WAR NIN G May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Symbols used

Depiction Meaning

1.

2.

Actions in the specified order

 Actions without a specified order

• List

Text Display text or software text

Additional information

Operating instructions

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Easypet

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English (EN)

1.4 Glossary

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Elapse time

Time required to empty a pipette from the upper part of the scale to the lowest scale
graduation or to fully empty a measuring pipette or volumetric pipette.

2 Product description

2.1 Main illustration

Abb. 2-1:Easypet 3 with accessories

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Product description

English (EN)

Easypet

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Fig. 2-1: Easypet 3 with accessories

1Shelf stand

2 Rechargeable battery status display

3 Aspiration button

4 Dispensing button

5 Rechargeable battery compartment lid

6 Connector socket

7 Aspirating cone

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6

8 Pipette adapter

9 Membrane filter

10 Seal for filter adapter

11 Filter adapter

12 Pressure compensation opening

13 Pipette clamp

Product description

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Easypet

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3

English (EN)

2.2 RFID chip

The Eppendorf dispensing device is equipped with a RFID chip. The RFID chip can be
read and written with the TrackIT reader and TrackIT software. The scanned device data
is saved in a database and can be opened at any time. The device data can be exported
individually or automatically in various formats.

2.2.1 RFID position

The position of the chip on dispensing devices is marked with the lettering RFID.

2.3 Delivery package

Quantity Description

1 Easypet 3

1 Li-polymer rechargeable battery

2 Non-sterile membrane filter, 0.45 μm

1Wall mount

1 Shelf stand (not available in the USA)

1 Universal power supply

1 Operating manual

1 Short operating manual

1CD

Product description

English (EN)

Easypet

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2.4 Warranty

For warranty claims, please contact your local Eppendorf sales partner. If the housing of
the pipetting aid is opened by unauthorized individuals, or the device is misused, no
warranty claim may be made. The rechargeable battery and all other wear parts are
excluded from the warranty.

2.5 Materials

NOTICE! Aggressive substances may damage the Easypet 3 and accessories.

 Check the resistance to chemicals before using organic solvents or aggressive

chemicals.

 Only use liquids without vapors which corrode the materials used.
 Observe the cleaning instructions.
 Additional information on chemical resistance can be found on the enclosed

CD or on our website www.eppendorf.com

The pipetting aid assemblies are composed of the following materials:

Component Material

Housing, aspirating cone, aspiration
button, dispensing button, membrane filter
housing, wall holder, shelf stands

Filter adapter Polybutylene terephthalate (PBT)

Pipette adapter Silicone

Filter membrane Polytetrafluoroethylene (PTFE)

Seal for filter adapter Hydrated acrylonitrile butadiene rubber

Tubes and valves Polymer fluorine rubber (FKM),

Battery status display Cyclic olefin copolymer (COC)

Polypropylene (PP)

(HNBR)

polybutylene terephthalate (PBT),
polyphenylene sulfide (PPS), silicone

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Product description

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English (EN)

2.6 Features

The Easypet 3 is a battery-driven pipetting aid. You can use glass or plastic pipettes in a
volume range of 0.1 mL to 100 mL.

A pump generates underpressure or overpressure to aspirate or dispense the liquid. The
liquid can also be dispensed solely via the atmospheric pressure.

The aspirating and dispensing speed is controlled by how far the control buttons are
pressed in.

Safety

Easypet

English (EN)

3Safety

3.1 Intended use

The pipetting aid is intended for dispensing liquids. In-vivo applications (in or on the
human body) are not allowed.

The pipetting aid may only be operated by skilled personnel who have received the
appropriate training. All users must have read the operating manual carefully and must
have become familiar with the device's mode of operation.

3.2 Warnings for intended use

WARNING! Damage to health due to infectious liquids and pathogenic
germs.

 When handling infectious liquids and pathogenic germs, observe the national

regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.

 Wear personal protective equipment.
 For full instructions regarding the handling of germs or biological material of

risk group II or higher, please refer to the "Laboratory Biosafety Manual"
(Source: World Health Organization, current edition of the Laboratory
Biosafety Manual).

WARNING! Risk of explosion from potentially explosive atmospheres and
potentially explosive substances.

 Do not use the Easypet 3 in potentially explosive atmospheres.
 Do not operate the Easypet 3 in areas where explosive substances are

handled.

 Do not use the Easypet 3 to dispense explosive, readily flammable (flash point

< 21°C), highly flammable (flash point < 0°C) or highly reactive substances.

 Do not use the Easypet 3 for dispensing substances which could generate an

explosive atmosphere.

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WARNING! Damage to health due to toxic, radioactive or aggressive
chemicals.

 Wear personal protective equipment.
 Observe the national regulations for handling these substances.
 Observe the material safety data sheets and manufacturer's application notes.

Safety

Easypet

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English (EN)

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CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by
Eppendorf may impair the safety, functioning and precision of the device.
Eppendorf cannot be held liable or accept any liability for damage resulting from
the use of incorrect or non-recommended accessories and spare parts, or from
the improper use of such equipment.

 Only use accessories and original spare parts recommended by Eppendorf.

CAUTION! Danger to individuals due to careless use.

 Never point the opening of a Easypet 3 which is equipped with a pipette at

yourself or other persons.

 Only initiate liquid dispensing if it is safe to do so.
 With any dispensing task please ensure that you do not endanger yourself

and other persons.

NOTICE! Damage to the device due to penetration of liquids.

 Do not allow any liquids to penetrate the inside of the housing.
 If liquid has entered the inside of the housing, the inner parts may only be

repaired by Eppendorf AG service partners. Contact your local sales office
before returning any devices.

NOTICE! Damage to device from missing pipette.

 Use the Easypet 3 only if a pipette has been inserted.

If you would like to dispense readily or highly flammable liquids, we recommend
the use of a positive displacement system made up of manual Multipettes/
Repeaters and Combitips. Check the resistance to chemicals and observe the
safety notes before using the Multipette/Repeater.

3.3 Information on product liability

In the following cases, the designated protection of the device may be compromised.
Liability for any resulting property damage or personal injury is then transferred to the
operator:

• The device is not used in accordance with the operating manual.

• The device is used outside of its intended use.

• The device is used with accessories or consumables which are not recommended by

Eppendorf.

• The device is maintained or repaired by people not authorized by Eppendorf.

• The user makes unauthorized changes to the device.