Please read this operating manual completely before using the device for the first time.
Please view this operating manual as part of the product and keep it somewhere easily
accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our
website www.eppendorf.com
1.2 Warning signs and hazard icons
1.2.1Hazard icons
BiohazardExplosion
CutsToxic substances
Hazard point Material damage
1.2.2Degrees of danger
The degree of danger is a part of a safety note and distinguishes the possible results of
non-observance from each other.
In case of warranty claims, contact your local Eppendorf contractual partner. No warranty is
provided if the Easypet is misused or opened by unauthorized persons. The rechargeable battery
and all other wear parts are excluded from the warranty.
2.4 Materials
NOTICE! Aggressive substances may damage the Easypet and the accessories.
When using aggressive chemicals, check their compatibility with the materials
NOTICE!
The parts of the Easypet that can be accessed by the user are made of the following materials:
ComponentMaterial
Selection dial, housingAcrylonitrile styrene acrylate (ASA) and
Aspirating button, dispensing buttonAcrylonitrile butadiene styrene (ASA) and
used.
Observe the cleaning instructions (see Cleaning on p. 15).
Seal for filter adapterHydrated acrylonitrile butadiene rubber (HNBR)
Release buttonPolyoxymethylene (POM)
Tubes and valvesFluorocarbon rubber (FKM) and
Polyoxymethylene (POM)
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Eppendorf Easypet® — Operating manual
2.5 Features
The Easypet is a rechargeable battery operated pipetting aid. You can use pipettes made of
glass or plastic in a volume range of 0.1 mL to 100 mL.
A pump generates an overpressure or a vacuum for liquid aspiration or dispensing. Alternatively,
the liquid can be dispensed by atmospheric pressure alone.
The aspiration and dispensing speed can be adjusted by an adjustment dial.
3 Safety
3Safety
3.1 Intended use
The Easypet is intended for dispensing liquids. In vivo applications (applications in or on the
human body) are not permitted.
The Easypet may only be operated by trained specialists. All users must have read the operating
manual carefully and familiarized themselves with the device's mode of operation.
3.2 Warnings for intended use
WARNING! Damage to health due to handling infectious liquids and pathogenic
germs.
WARNING!
Observe the national regulations for handling these substances, the biological
security level of your laboratory, the material safety data sheets and the
manufacturer's application notes.
Wear personal protective equipment (PPE).
Follow the instructions regarding hygiene, cleaning and decontamination.
For complete instructions regarding the handling of germs or biological material of
risk group II or higher, please refer to the "Laboratory Biosafety Manual" (source:
World Health Organization, current edition of the Laboratory Biosafety Manual).
WARNING! Risk of explosion from potentially explosive atmospheres and
potentially explosive substances.
WARNING!
Do not use the Easypet in potentially explosive atmospheres.
Do not operate the Easypet in rooms where work is being carried out with explosive
substances.
No explosive, flammable (flash point < 21°C), highly flammable (flash point < 0°C)
or highly reactive materials may be dispensed using the Easypet.
Do not use the Easypet to process any substances which could create an explosive
atmosphere.
WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.
Observe the national regulations for handling these substances as well as the
WARNING!
material safety data sheets and manufacturer's application notes.
Wear personal protective equipment (PPE).
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Eppendorf Easypet® — Operating manual
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf
CAUTION!
may impair the safety, functioning and precision of the device. Eppendorf cannot be
held liable or accept any liability for damage resulting from the use of incorrect or
non-recommended accessories and spare parts or from the improper use of such
equipment.
Only use accessories and original spare parts recommended by Eppendorf.
CAUTION! Danger to persons from careless use.
Never point the opening of a Easypet equipped with a pipette at yourself or other
CAUTION!
NOTICE!
NOTICE!
persons.
Only initiate dispensing if it is safe to do so.
For all dispensing tasks, make sure that you are not endangering yourself or
anyone else.
NOTICE! Damage to device from penetration of liquids.
Do not allow any liquids to penetrate the inside of the housing.
If liquid has penetrated the inside of the housing, have the inner parts of the
Easypet repaired only by service partners of Eppendorf AG. Contact your
responsible sales office before returning a product.
NOTICE! Damage to device from missing pipette.
Only use the Easypet with a pipette inserted.
3.3 Information on product liability
In the following cases, the protection provided by the device may be impaired. The liability for the
function of the device passes to the operator if:
• The device is not used in accordance with the operating manual.
• The device is used outside of the range of application described in the preceding chapters.
• The owner has made unauthorized modifications to the device.
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Eppendorf Easypet® — Operating manual
4 Installation
4Installation
4.1 Preparing installation
Use the delivery package details to check that the delivery is complete.
Check all parts for any transport damage.
Keep the transport carton and the packing material for subsequent safe transport or storage.
4.2 Loading the rechargeable battery
WARNING! Use of incorrect power supplies may result in fatal injuries and
damage to the device.
WARNING!
Use of incorrect power supplies may result in overheating, burning, melting,
short-circuiting of the Easypet or similar damage.
Only use the supplied power supply for charging the device.
Only connect the power supply to voltage sources that correspond to the
information on the identification plate regarding the power supply.
Do not use the power supply when signs of damage are evident, and particularly if
the power cable is damaged.
WARNING! Incorrect charging of the rechargeable battery can cause serious
personal injuries and damage to the device.
WARNING!
Only use the rechargeable batteries supplied.
Do not use any primary cells!
Do not charge the NiMH rechargeable batteries outside of the Easypet.
Do not connect the Easypet to the power supply without the rechargeable batteries
inserted.
WARNING! Personal injury caused by the incorrect handling of the rechargeable
battery.
WARNING!
Do not disassemble or modify the rechargeable battery.
Never pierce, crush or throw the rechargeable battery.
Only use the rechargeable battery in the Easypet.
Do not touch leaky rechargeable batteries.
Do not use any damaged rechargeable batteries.
Dispose of damaged rechargeable batteries in accordance with legal regulations.
On delivery the nickel-metal hydride rechargeable batteries (NiMH rechargeable batteries) are
already inserted. Fully charge the rechargeable batteries before using the device for the first
time. If using the device daily, do not charge the rechargeable batteries until the charge control
lamp has lit up (see Fig. 1 on p. 6). A residual charge of approximately 15% is still available when
the lamp is lit.
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Easypet
Eppendorf Easypet® — Operating manual
To charge the rechargeable batteries, proceed as follows:
1. Insert the power supply into the socket.
2. Insert the charging plug of the power supply into the connector socket on the grip (see Fig. 1
on p. 6).
The red charge control lamp on the grip goes out. The charging time depends on the
charging state of the rechargeable batteries. Fully discharged rechargeable batteries require
a charging time of about 12 hours.
You can also use the Easypet during the charging process.
Hint!
4.3 Storage
The Easypet can be stored in a wall holder.
Fastening the wall holder
1. Clean the fastening point on the wall.
2. Remove the protective foil.
3. Press the wall holder firmly against the wall.
Removing the wall holder
1. Pull the wall holder to one side to remove it
from the wall.
2. Remove the fastening strip with the aid of
commonly-used solvents.
Abb. 2:Easypet with wall holder
Fig. 2: Easypet with wall holder
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Eppendorf Easypet® — Operating manual
5Operation
5Operation
5.1 Inserting the pipette
WARNING! Risk of cuts from shattered glass pipettes.
Glass pipettes are fragile and may cause severe cuts when breaking.
WARNING!
Do not use force to insert glass pipettes.
Wear personal protective equipment (PPE).
Protect the hand you are using for insertion with a towel.
Holding the pipette at the top, push it
carefully into the aspirating cone until it is
securely fastened and air tight.
Abb. 3:Inserting the pipette
12
Fig. 3: Inserting the pipette
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Easypet
Eppendorf Easypet® — Operating manual
5.2 Aspirating liquid
NOTICE! Damage to device due to missing or damaged membrane filter.
Easypet must not be used without the membrane filter inserted.
NOTICE!
Replace the membrane filter if it is damaged.
1. The aspiration speed can be continuously
adjusted using the adjustment dial.
I = slow, IIIII = fast
2. Immerse the pipette into the liquid.
3. Press the aspirating button until the pipette
is filled just above the desired mark.
4. Press the dispensing button slowly until the
meniscus is exactly level with the desired
mark.
Abb. 4:Aspirate liquid
Fig. 4: Aspirate liquid
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Easypet
Eppendorf Easypet® — Operating manual
5.3 Dispensing liquid
Flow-out
During flow-out, a valve is opened. In this mode,
liquid dispensing takes place without activating
the pump, only using atmospheric pressure.
1. Place the pipette against the wall of the
vessel.
2. Press the dispensing button down to 5 mm.
Vary the pressure in order to change the
dispensing speed.
Blow-out
During blow-out, liquid is dispensed with the aid
of the pump.
1. In this mode, the dispensing speed can be
continuously adjusted using the adjustment
dial.
2. Place the pipette against the wall of the
vessel.
3. Press in the dispensing button completely.
Abb. 5:Dispense liquid
14
Fig. 5: Dispense liquid
Page 14
Eppendorf Easypet® — Operating manual
6 Maintenance
6Maintenance
6.1 Cleaning
No special maintenance is required.
Hint!
NOTICE! Damage to the device due to autoclaving.
Do not autoclave the Easypet.
NOTICE!
To clean contaminated surfaces, proceed as follows.
The housing can be wiped with a moist cloth.
The surfaces can be disinfected using alcohol (ethanol, propanol) or with a disinfectant
containing alcohol.
6.2 Replacing the rechargeable batteries
1. Slide open the rechargeable battery compartment cover in the grip
2. Take out the old or damaged rechargeable batteries.
3. Insert the new rechargeable batteries into the rechargeable battery compartment according
to the markings.
4. Close the rechargeable battery compartment.
5. Charge the rechargeable batteries.
If your Easypet still contains NiCd rechargeable batteries, you can replace them with
Hint!
NiMH rechargeable batteries.
6.3 Dismantling the pipette clamp
If liquid has entered the pipette clamp, aspiration capacity may be reduced or pipette clamp parts
may be damaged. To clean or replace the parts, it will be necessary to dismantle the pipette
clamp.
1. While keeping the release button pressed, unscrew and remove the aspirating cone
counterclockwise.
2. Remove the pipette adapter with the membrane filter from the filter adapter.
3. Remove the membrane filter from the pipette adapter.
4. Pry the seal with a sharp object out of the filter adapter.
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Eppendorf Easypet® — Operating manual
6.4 Cleaning the pipette clamp
The parts of the pipette clamp can be replaced or cleaned as follows.
Requirement
Easypet is disassembled (see p. 15).
1. Replace the part if necessary.
2. Clean the part if necessary. Autoclave the aspirating cone and the pipette adapter at 121°C,
1 bar overpressure for 20 min if necessary. Then allow to dry at room temperature.
Aspirating cone
• Can be wiped with a moist cloth
• Can be disinfected with alcohol (ethanol,
propanol) or with a disinfectant containing
alcohol
Pipette adapter
• Can be rinsed with demineralized water
• Can be steam autoclaved multiple times
• Can be replaced
• Can be steam autoclaved multiple times
Membrane filter
Sealing
• Can be replaced
• Can be steam autoclaved once
• Dispose of if contaminated
• Cannot be cleaned
• Can be replaced
Alternatively, you can also use a membrane filter with a pore size of 0.2 μm, for
Hint!
example, type Minisart SRP from Sartorius stedim.
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Eppendorf Easypet® — Operating manual
6.5 Mounting the pipette clamp
1. Press the seal into the filter adapter.
2. Press the wide opening of the membrane
filter into the narrower opening of the filter
adapter.
3. Insert the assembled pipette adapter with the membrane filter into the seal of the filter
adapter.
4. Put the aspirating cone over the assembled pipette adapter with the membrane filter and
screw in until it engages.
5. Check that the Easypet is leak-tight.
Hold the Easypet vertically with the filled pipette for approx. 30 s. Do not touch the pipette tip.
Observe the meniscus of the liquid on the tip opening. If there is a leak in the pipette, a drip
will form on the tip opening.
When the pipette is filled, no liquid should drip out of the pipette for 30 s as long as the
aspirating or dispensing buttons are not pressed. If liquid does drip out, dismantle the pipette
adapter with the membrane filter and carefully reassemble the Easypet.
7 Troubleshooting
7Troubleshooting
7.1 General errors
Symptom /
message
Liquid drips out of
the pipette.
Aspiration capacity
reduced.
Short operation
time despite fully
charged
rechargeable
batteries.
Pipette loose.
CauseRemedy
• Pipette adapter and/or
membrane filter inserted
incorrectly.
Remove pipette adapter and
membrane filter and insert
again.
• Pipette not inserted far enough. Carefully insert pipette further.
battery
Capacity: 600 mAh/1.2 V
Charging time: ~12 h
Operation period: ~7 h
Number of charge cycles: ~500
Weight: ~15.5 g per rechargeable battery
• 230 V, 50 Hz (EU)
• 120 V, 60 Hz (USA)
• 230 V, 50 Hz (UK)
• 100 V, 50/60 Hz (JA)
• 240 V, 50 Hz (AUS)
Output voltage: 9 V, 200 mA
Air temperature: -25 to 55°C
Rel. air humidity: 10 to 95%
Atmospheric pressure: 70 to 106 kPa
Air temperature: -5 to 45°C
Rel. air humidity: 10 to 95%
Atmospheric pressure: 70 to 106 kPa
4421 601.009022231006
4421 602.0054421602005Seal for filter adapter (5 pieces)
4420 810.003022236016
4850 868.005
4850 869.001
4850 871.006
0013 611.955
4420 815.005022230905
Order No.
(North America)
022230204
022236300
Description
Eppendorf Easypet
Incl. power supply, wall holder,
2 membrane filters (unsterile) 0.45 μm
230 V / 50 Hz (EU)
120 V / 60 Hz (USA)
230 V / 50 Hz (UK)
240 V / 50 Hz (AU)
100 V / 50/60 Hz (JP)
Membrane filter
0.45 μm, sterile, 1 set (pack of five)
NiMH accumulator
3 pieces, for Easypet
Power supply
9 V, for Easypet
230 V / 50 Hz (EU)
120 V / 60 Hz (USA)
230 V / 50 Hz (UK)
100 V / 50/60 Hz (JP)
240 V / 50 Hz (AU)
Wall mounting device
For Easypet
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Eppendorf Easypet® — Operating manual
10 Transport, storage and disposal
10Transport, storage and disposal
10.1 Decontamination before shipment
Decontaminate the pipette before shipping it to the authorized Technical Service for repairs or to
your authorized dealer for disposal. Please note the following:
If liquid has penetrated into the Easypet through the membrane filter, decontamination of the
internal parts of the Easypet can only be carried out by the authorized service partner of
Eppendorf AG. In this case the enclosed decontamination certificate must include the following
additional information:
• An exact description of the substances which have entered the device (including a copy of
the respective safety data sheets if possible)
• The serial number (can be found in the rechargeable battery compartment)
• A description that is easy to understand and carry out by non-specialists for decontamination
and, if necessary, for proper disposal of the decontaminated internal parts.
Prior to shipment contact your service partner to check whether decontamination is possible.
WARNING! Risk to health from contaminated device
1. Follow the instructions in the decontamination certificate. It is available in PDF
WARNING!
format on our homepage (www.eppendorf.com/decontamination)
2. Decontaminate all the parts you want to dispatch.
3. Enclose the fully-completed decontamination certificate for returned goods
(including the serial number of the device) with the dispatch.
.
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Eppendorf Easypet® — Operating manual
10.2 Disposal
Always observe the statutory requirements when disposing of the product.
Information on the disposal of electrical and electronic devices in the European
Community:
Within the European Community, the disposal of electrical devices is regulated by national
regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic
equipment (WEEE).
According to these regulations, any devices supplied after August 13, 2005, in the
business-to-business sphere, to which this product is assigned, may no longer be disposed of in
municipal or domestic waste. They are marked with the following symbol to indicate this:
As disposal regulations may differ from country to country within the EU, please contact
your supplier if necessary.
In Germany, this is mandatory from March 23, 2006. From this date, the manufacturer
has to offer a suitable method of return for all devices supplied after August 13, 2005.
For all devices supplied before August 13, 2005, the last user is responsible for the
correct disposal.
WARNING! Risk of explosion and fire from overheated batteries.
Do not heat the batteries above 80 °C and do not throw them into the fire.
WARNING!
Disposing of rechargeable batteries
Do not dispose of rechargeable batteries as household waste. Dispose of rechargeable
batteries in compliance with local legal disposal regulations.