Eppendorf E-25R User Manual
1
New Brunswick Biological Shakers
®
Excella
Operating Manual
E-25/25R
M1353-0050
Revision D
2
COPYRIGHT:
Copyright © 2011 New Brunswick Scientific Co., Inc., USA.
No part of this publication may be reproduced without the prior permission of the copyright
owner.
New Brunswick Scientific reserves the right to change information in this document
without notice. Updates to information in this document reflect our commitment to
continuing product development and improvement.
TRADEMARKS:
Excella
Inc., USA.
The eppendorf logo, New Brunswick™ and the New Brunswick Logo™ are trademarks
of Eppendorf AG, Hamburg, Germany.
Trademarks are not marked in all cases with ™ or ® in this manual.
New Brunswick Scientific has attempted to identify the ownership of all trademarks from public
records. Any omissions or errors are unintentional.
®
and Innova® are trademarks owned and registered by New Brunswick Scientific Co.,
November 16, 2011
Revision D
M1353-0050
M1353-0050 Excella® E-25/E25R Classic Incubator Shakers Operating Manual
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CAUTION! Risk of damage to personnel and/or equipment!
This equipment must be operated as described in this manual.
Please read the entire Operating Manual before attempting to use this
equipment. If operational guidelines are not followed, equipment damage and
personal injury can occur.
Do not use this equipment in a hazardous atmosphere or with hazardous
materials for which the equipment was not designed.
New Brunswick Scientific Co., Inc. is not responsible for any damage to this
equipment that may result from the use of an accessory not manufactured by
New Brunswick.
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WARRANTY
New Brunswick Scientific equipment is protected by a comprehensive warranty.
The warranty covers faulty components and assembly, and our obligation under this
warranty is limited to repairing or replacing the instrument or part thereof, which shall
prove to be defective after our examination.
The warranty does not cover loss of time or materials, such as the loss of biological or
biochemical by-products caused by any work interruption resulting from equipment failure;
it does not extend to equipment that has been subject to misuse, neglect, accident or
improper installation or application; nor does it cover any machine that has been repaired
or altered by anyone other than an authorized New Brunswick Scientific factory-trained
service representative, without prior written approval from your local New Brunswick sales
office or distributor.
Expendable items such as bearings and seals, lamps, probes, sensors including incubator
sensors, glass, filters, single-use vessels, etc., are not covered.
The warranty begins on the date the equipment ships from New Brunswick Scientific or an
authorized distributor and extends through the period indicated in the chart below:
Instrument Parts Warranty Labor Warranty
Innova 3 years 2 years
Shakers
Freezers
Fermentors, Bioreactors & all
other New Brunswick equipment
1 Photosynthetic light banks, etc.
2 Stacking stand, casters, shelves, etc.
3 Chart recorders, CO
Warranty Registration
To register your warranty, complete the online form at www.nbsc.com /warranty..
Extended Warranty Option
A variety of service plans are offered to help minimize downtime from unexpected malfunctions
in equipment operation. Speak to your New Brunswick sales representative for more
information.
I Series 2 years 2 years
Excella & C-76 2 years 2 years
Accessories
1
1 year 1 year
Incubators 2 years 2 years CO2 Incubators
2
Accessories
1 year 1 year
5 years;
ULT Freezers
Vacuum insulation
2 years
panels: 12 years
Accessories
3
1 year 1 year
1 year
/LN2 back-up systems, etc.
2
1 year
M1353-0050 Excella® E-25/E25R Classic Incubator Shakers Operating Manual
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TABLE OF CONTENTS
1 OVERVIEW.......................................................................................................................... 7
1.1 SPECIFICATIONS............................................................................................................... 8
1.2 CERTIFICATIONS ..............................................................................................................9
2 INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT........................ 11
2.1 INSPECTION OF BOXES ...................................................................................................11
2.2 UNPACKING ................................................................................................................... 11
2.3 INSPECTION OF EQUIPMENT ........................................................................................... 11
2.4 OUT OF BOX CONCERNS ................................................................................................ 12
2.5 WARRANTY REGISTRATION........................................................................................... 12
3 PREPARING THE LOCATION....................................................................................... 13
3.1 PHYSICAL LOCATION ..................................................................................................... 13
3.2 ENVIRONMENT............................................................................................................... 13
3.3 ELECTRICAL REQUIREMENTS......................................................................................... 13
3.4 SPACE REQUIREMENTS .................................................................................................. 14
4 FEATURES......................................................................................................................... 15
4.1 FRONT PANEL ................................................................................................................ 15
5 GETTING STARTED........................................................................................................ 18
5.1 INSTALLATION OF PLATFORM ........................................................................................ 18
5.2 INSTALLING FLASK CLAMPS .......................................................................................... 19
5.3 ELECTRICAL CONNECTIONS........................................................................................... 20
6 OPERATION ...................................................................................................................... 22
6.1 STARTING THE SHAKER.................................................................................................. 22
6.2 C
6.3 C
6.4 T
ONTINUOUS (UNLIMITED) RUN.................................................................................... 22
HECKING A SETPOINT .................................................................................................. 23
IMED FUNCTIONS......................................................................................................... 23
6.5 ALARM FUNCTIONS........................................................................................................ 24
6.6 TEMPERATURE SETPOINT............................................................................................... 25
6.7 T
6.8 P
EMPERATURE OFFSET CALIBRATION ........................................................................... 25
OWER FAILURE ............................................................................................................ 26
6.9 SPEED CALIBRATION...................................................................................................... 26
6.10 M
AXIMUM RECOMMENDED SPEED ................................................................................ 27
7 PREVENTIVE MAINTENANCE.....................................................................................28
7.1 C
LEANING EXTERNAL SURFACES................................................................................... 28
7.2 FUSE REPLACEMENT...................................................................................................... 28
7.3 B
ELT REPLACEMENT...................................................................................................... 31
8 TROUBLESHOOTING..................................................................................................... 33
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9 REPLACEMENT PARTS & ACCESSORIES................................................................ 35
9.1 R
9.2 A
EPLACEMENT PARTS.................................................................................................... 35
CCESSORIES................................................................................................................. 35
10 DRAWINGS.................................................................................................................... 37
10.1 L
10.2 L
IST OF DRAWINGS........................................................................................................ 40
IST OF TABLES ............................................................................................................. 41
11 INDEX.............................................................................................................................. 42
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The Excella® E-25 Classic Incubator and E-25R Classic Refrigerated Incubator Shakers are large
capacity shakers utilizing a UniCentric single eccentric counterbalanced drive to provide
horizontal plane rotary motion in a 2.54 cm (1 in) circular orbit. A Proportional/Integral (PI)
Microprocessor controller with instantaneous digital feedback controls the speed over a range of
50-400 rpm.
The E-25 provides temperature control over a range of 7C above ambient to 60C, while the
E-25R provides temperature control over a range of 15C below ambient (minimum 4C) to
60C. Ambient temperature is defined as the temperature within one meter of the shaker.
The shakers may be operated either continuously or in a timed mode via a programmable timer
for shaking periods of 0.1 hr. to 99.9 hrs.
For safe operation, both the E-25 and E-25R are designed with a safety switch that automatically
stops the shaker mechanism when the lid is lifted.
In addition, the Excella E-25 and E-25R are equipped with visual and audible alarms that alert
the user to the following conditions:
The end of a timed run
Deviations of shaking speed
Deviations of temperature setpoint
Power failure
Lid open
A wide variety of platforms can be used with the E-25 or E-25R. Dedicated platforms are
available for a variety of flask sizes. Universal platforms, utility trays, utility carriers and test
tube racks are also available.
O
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1.1 Specifications
Excella E-25 & E-25R Incubator Shaker
SHAKING
Speed
1
Control Accuracy
Indication
Stroke/Orbit
TEMPERATURE
Range (E-25)
Range (E-25R)
Control Accuracy
Indication
Ambient* Operating
Environment
Refrigerant (E-25R only)
Alarms
Timer
Automatic Restart
Drive Interrupt
Electrical Requirements
ETL Regulatory
Standards
CE Regulatory Standards
Platform
Overall Dimensions
Chamber Dimensions
Net Weight
Gross Weight
1
See NOTE below.
* Ambient temperature is defined as the temperature measured one meter from the
shaker.
50-400 rpm
1 rpm
3-digit LED, displayed in 1 rpm increments
2.54 cm (1 in)
7°C above ambient temperature* to 60°C
15°C below ambient temperature* (min. 4°C) to 60°C
0.1°C @ 37°C
3-digit LED, displayed in 0.1°C increments
10 - 35°C, 20 to 80% relative humidity, non-condensing
R-134A
Visible and audible warning indication when speed deviates
more than 5 rpm, and temperature more than 1°C from
setpoints, and when timer has expired.
0.1 to 99.9 hours. Shuts off agitation at end of period. Can
be deactivated for continuous operation.
Automatic restart after power is restored. Setpoints and
operating status are retained in memory during power
interruption.
Automatic drive-interrupt when cover is opened.
110/120 VAC, 50/60 Hz
220/240 VAC, 50/60 Hz
E-25: 800 VA per shaker
E-25R: 1500 VA per shaker
UL 61010A-1 CAN/CSA-C22.2 No. 1010.1
UL 61010A-2-010 CAN/CSA-C22.2 No. 1010.2.010
See Declaration of Conformity, Section 1.2
76 X 46 cm (30 X 18 in)
127 cm W X 77 cm D X 85 cm H (50 in X 30.5 in X 41.7 in)
85 cm W X 58 cm D X 48 cm H clearance above platform
(33.5 in X 23 in X 19 in)
E-25: 191 kg (420 lb), E-25R: 204.5 kg (450 lb)
E-25: 204.5 kg (450 lb), E-25R: 281 kg (480 lb)
NOTE:
Use of baffled flasks will significantly reduce maximum speed for any
shaker. We may be able to improve this maximum speed by using an
alternative motor pulley. Contact your New Brunswick representative for
more information.
M1353-0050 Excella® E-25/E25R Classic Incubator Shakers Operating Manual
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1.2 Certifications
The Excella E-25 & E-25R have been tested to ETL standards, to comply with UL and
CAN/CSA electrical safety standards (see “ETL Regulatory Standards” in the
specifications table, Section 1.1).
As attested in the CE Declaration of Conformity reproduced on the following page, they
also conform to the appropriate CE standards (see also “CE Regulatory Standards” in the
specifications table).
New Brunswick Scientific Operating Manual
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M1353-0050 Excella® E-25/E25R Classic Incubator Shakers Operating Manual
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22
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2.1 Inspection of Boxes
After you receive your order from New Brunswick Scientific, inspect the boxes carefully
for any damage that may have occurred during shipping. Report any damage to the
carrier and to your local New Brunswick Sales Order Department.
2.2 Unpacking
Save all packing materials and Operating Manual. If any part of your order was damaged
during shipping, missing pieces, or fails to operate properly, contact your New
Brunswick sales representative.
CRUSH WARNING!
Do not attempt to lift the E-25 or E-25R by hand. Always use a lifter or
suitable equipment when raising the shaker.
E
QQUUIIPPMMEENNT
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2.3 Inspection of Equipment
Verify against your New Brunswick packing list that you have received the correct
materials.
You should have received a Power Kit (power cord, fuse, hex wrench and key) with your
shaker. Make sure your electrical supply matches the electrical specification of the
power kit and the shaker (both of which should be the same).
NOTE:
Use of the Excella E-25/25R Shakers requires a platform, which is a
separate item. See the Available Platforms list in Section 9.2.
New Brunswick Scientific Operating Manual
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2.4 Out of Box Concerns
If any part of your order was damaged during shipping, is missing pieces, or fails to
operate properly, please contact your sales representative.
2.5 Warranty Registration
Please register electronically at our Website: www.nbsc.com
M1353-0050 Excella® E-25/E25R Classic Incubator Shakers Operating Manual
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3.1 Physical Location
It is essential that the instrument be situated in a area where there is sufficient space for
the shaker and platform to clear walls and obstructions during operation. The surface on
which the shaker is placed must be smooth, level, and able to support the shaker under
full load operating conditions.
CRUSH WARNING!
Do not attempt to lift the E-25 or E-25R by hand. Always use a lifter or
suitable equipment when raising the shaker.
The feet can be adjusted for necessary leveling. Loosen the locking nuts on the threaded
studs attached to the feet of the shaker. Retighten when you have achieved the correct
level for your shaker.
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3.2 Environment
The shaker is designed to operate optimally in the following ambient conditions:
10 - 35°C
20 to 80% Relative Humidity non-condensing
3.3 Electrical Requirements
The E-25 shakers can be equipped to run on:
100 Volts, 50/60 Hz, 800 VA maximum
120 Volts, 60 Hz, 800 VA maximum
230 Volts, 50 Hz, 800 VA maximum
The E-25R shakers can be equipped to run on:
100 Volts, 50/60 Hz, 1500 VA maximum
120 Volts, 60 Hz, 1500 VA maximum
230 Volts, 50 Hz, 1500 VA maximum
New Brunswick Scientific Operating Manual
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In all cases, voltage variation must not exceed ± 10%.
3.4 Space Requirements
Figure 1: Space Requirements
1
2
3
4
5
1 Height with lid open: 165.7 cm (65¼ in)
2 10 cm (4 in) front & left side clearance
3 127 cm (50 in)
4 87.6 cm (34½ in) front to back clearance
5 61 cm (24 in) for access to service door
M1353-0050 Excella® E-25/E25R Classic Incubator Shakers Operating Manual
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