Eppendorf E-25R User Manual

1

New Brunswick Biological Shakers

®

Excella

Operating Manual

E-25/25R

M1353-0050

Revision D

2

COPYRIGHT:

Copyright © 2011 New Brunswick Scientific Co., Inc., USA.
No part of this publication may be reproduced without the prior permission of the copyright
owner.

New Brunswick Scientific reserves the right to change information in this document
without notice. Updates to information in this document reflect our commitment to
continuing product development and improvement.

TRADEMARKS:

Excella
Inc., USA.

The eppendorf logo, New Brunswick™ and the New Brunswick Logo™ are trademarks
of Eppendorf AG, Hamburg, Germany.

Trademarks are not marked in all cases with ™ or ® in this manual.
New Brunswick Scientific has attempted to identify the ownership of all trademarks from public

records. Any omissions or errors are unintentional.

®

and Innova® are trademarks owned and registered by New Brunswick Scientific Co.,

November 16, 2011
Revision D
M1353-0050

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

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CAUTION! Risk of damage to personnel and/or equipment!

 This equipment must be operated as described in this manual.
 Please read the entire Operating Manual before attempting to use this

equipment. If operational guidelines are not followed, equipment damage and
personal injury can occur.

 Do not use this equipment in a hazardous atmosphere or with hazardous

materials for which the equipment was not designed.

 New Brunswick Scientific Co., Inc. is not responsible for any damage to this

equipment that may result from the use of an accessory not manufactured by
New Brunswick.

New Brunswick Scientific Operating Manual

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WARRANTY

New Brunswick Scientific equipment is protected by a comprehensive warranty.
The warranty covers faulty components and assembly, and our obligation under this
warranty is limited to repairing or replacing the instrument or part thereof, which shall
prove to be defective after our examination.

The warranty does not cover loss of time or materials, such as the loss of biological or
biochemical by-products caused by any work interruption resulting from equipment failure;
it does not extend to equipment that has been subject to misuse, neglect, accident or
improper installation or application; nor does it cover any machine that has been repaired
or altered by anyone other than an authorized New Brunswick Scientific factory-trained
service representative, without prior written approval from your local New Brunswick sales
office or distributor.

Expendable items such as bearings and seals, lamps, probes, sensors including incubator
sensors, glass, filters, single-use vessels, etc., are not covered.

The warranty begins on the date the equipment ships from New Brunswick Scientific or an
authorized distributor and extends through the period indicated in the chart below:

Instrument Parts Warranty Labor Warranty

Innova 3 years 2 years

Shakers

Freezers

Fermentors, Bioreactors & all
other New Brunswick equipment

1 Photosynthetic light banks, etc.
2 Stacking stand, casters, shelves, etc.
3 Chart recorders, CO

Warranty Registration
To register your warranty, complete the online form at www.nbsc.com /warranty..

Extended Warranty Option

A variety of service plans are offered to help minimize downtime from unexpected malfunctions
in equipment operation. Speak to your New Brunswick sales representative for more
information.

I Series 2 years 2 years
Excella & C-76 2 years 2 years
Accessories

1

1 year 1 year

Incubators 2 years 2 years CO2 Incubators

2

Accessories

1 year 1 year

5 years;

ULT Freezers

Vacuum insulation

2 years

panels: 12 years

Accessories

3

1 year 1 year

1 year

/LN2 back-up systems, etc.

2

1 year

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

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TABLE OF CONTENTS

1 OVERVIEW.......................................................................................................................... 7

1.1 SPECIFICATIONS............................................................................................................... 8

1.2 CERTIFICATIONS ..............................................................................................................9

2 INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT........................ 11

2.1 INSPECTION OF BOXES ...................................................................................................11

2.2 UNPACKING ................................................................................................................... 11

2.3 INSPECTION OF EQUIPMENT ........................................................................................... 11

2.4 OUT OF BOX CONCERNS ................................................................................................ 12

2.5 WARRANTY REGISTRATION........................................................................................... 12

3 PREPARING THE LOCATION....................................................................................... 13

3.1 PHYSICAL LOCATION ..................................................................................................... 13

3.2 ENVIRONMENT............................................................................................................... 13

3.3 ELECTRICAL REQUIREMENTS......................................................................................... 13

3.4 SPACE REQUIREMENTS .................................................................................................. 14

4 FEATURES......................................................................................................................... 15

4.1 FRONT PANEL ................................................................................................................ 15

5 GETTING STARTED........................................................................................................ 18

5.1 INSTALLATION OF PLATFORM ........................................................................................ 18

5.2 INSTALLING FLASK CLAMPS .......................................................................................... 19

5.3 ELECTRICAL CONNECTIONS........................................................................................... 20

6 OPERATION ...................................................................................................................... 22

6.1 STARTING THE SHAKER.................................................................................................. 22

6.2 C

6.3 C

6.4 T

ONTINUOUS (UNLIMITED) RUN.................................................................................... 22

HECKING A SETPOINT .................................................................................................. 23

IMED FUNCTIONS......................................................................................................... 23

6.5 ALARM FUNCTIONS........................................................................................................ 24

6.6 TEMPERATURE SETPOINT............................................................................................... 25

6.7 T

6.8 P

EMPERATURE OFFSET CALIBRATION ........................................................................... 25

OWER FAILURE ............................................................................................................ 26

6.9 SPEED CALIBRATION...................................................................................................... 26

6.10 M

AXIMUM RECOMMENDED SPEED ................................................................................ 27

7 PREVENTIVE MAINTENANCE.....................................................................................28

7.1 C

LEANING EXTERNAL SURFACES................................................................................... 28

7.2 FUSE REPLACEMENT...................................................................................................... 28

7.3 B

ELT REPLACEMENT...................................................................................................... 31

8 TROUBLESHOOTING..................................................................................................... 33

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9 REPLACEMENT PARTS & ACCESSORIES................................................................ 35

9.1 R

9.2 A

EPLACEMENT PARTS.................................................................................................... 35

CCESSORIES................................................................................................................. 35

10 DRAWINGS.................................................................................................................... 37

10.1 L

10.2 L

IST OF DRAWINGS........................................................................................................ 40

IST OF TABLES ............................................................................................................. 41

11 INDEX.............................................................................................................................. 42

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11

The Excella® E-25 Classic Incubator and E-25R Classic Refrigerated Incubator Shakers are large
capacity shakers utilizing a UniCentric single eccentric counterbalanced drive to provide
horizontal plane rotary motion in a 2.54 cm (1 in) circular orbit. A Proportional/Integral (PI)
Microprocessor controller with instantaneous digital feedback controls the speed over a range of
50-400 rpm.

The E-25 provides temperature control over a range of 7C above ambient to 60C, while the
E-25R provides temperature control over a range of 15C below ambient (minimum 4C) to
60C. Ambient temperature is defined as the temperature within one meter of the shaker.

The shakers may be operated either continuously or in a timed mode via a programmable timer
for shaking periods of 0.1 hr. to 99.9 hrs.

For safe operation, both the E-25 and E-25R are designed with a safety switch that automatically
stops the shaker mechanism when the lid is lifted.

In addition, the Excella E-25 and E-25R are equipped with visual and audible alarms that alert
the user to the following conditions:

 The end of a timed run
 Deviations of shaking speed
 Deviations of temperature setpoint
 Power failure
 Lid open

A wide variety of platforms can be used with the E-25 or E-25R. Dedicated platforms are
available for a variety of flask sizes. Universal platforms, utility trays, utility carriers and test
tube racks are also available.

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1.1 Specifications

Excella E-25 & E-25R Incubator Shaker

SHAKING
Speed

1

Control Accuracy
Indication
Stroke/Orbit
TEMPERATURE
Range (E-25)
Range (E-25R)
Control Accuracy
Indication
Ambient* Operating
Environment
Refrigerant (E-25R only)

Alarms

Timer

Automatic Restart
Drive Interrupt

Electrical Requirements

ETL Regulatory
Standards
CE Regulatory Standards
Platform
Overall Dimensions
Chamber Dimensions

Net Weight
Gross Weight

1

See NOTE below.
* Ambient temperature is defined as the temperature measured one meter from the
shaker.

50-400 rpm
 1 rpm
3-digit LED, displayed in 1 rpm increments

2.54 cm (1 in)
7°C above ambient temperature* to 60°C

15°C below ambient temperature* (min. 4°C) to 60°C
 0.1°C @ 37°C
3-digit LED, displayed in 0.1°C increments
10 - 35°C, 20 to 80% relative humidity, non-condensing

R-134A
Visible and audible warning indication when speed deviates

more than 5 rpm, and temperature more than 1°C from
setpoints, and when timer has expired.

0.1 to 99.9 hours. Shuts off agitation at end of period. Can
be deactivated for continuous operation.

Automatic restart after power is restored. Setpoints and
operating status are retained in memory during power
interruption.
Automatic drive-interrupt when cover is opened.

110/120 VAC, 50/60 Hz
220/240 VAC, 50/60 Hz

E-25: 800 VA per shaker

E-25R: 1500 VA per shaker
UL 61010A-1 CAN/CSA-C22.2 No. 1010.1
UL 61010A-2-010 CAN/CSA-C22.2 No. 1010.2.010
See Declaration of Conformity, Section 1.2
76 X 46 cm (30 X 18 in)
127 cm W X 77 cm D X 85 cm H (50 in X 30.5 in X 41.7 in)
85 cm W X 58 cm D X 48 cm H clearance above platform
(33.5 in X 23 in X 19 in)
E-25: 191 kg (420 lb), E-25R: 204.5 kg (450 lb)
E-25: 204.5 kg (450 lb), E-25R: 281 kg (480 lb)

NOTE:

Use of baffled flasks will significantly reduce maximum speed for any
shaker. We may be able to improve this maximum speed by using an
alternative motor pulley. Contact your New Brunswick representative for
more information.

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1.2 Certifications

The Excella E-25 & E-25R have been tested to ETL standards, to comply with UL and
CAN/CSA electrical safety standards (see “ETL Regulatory Standards” in the
specifications table, Section 1.1).

As attested in the CE Declaration of Conformity reproduced on the following page, they
also conform to the appropriate CE standards (see also “CE Regulatory Standards” in the
specifications table).

New Brunswick Scientific Operating Manual

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M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

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2.1 Inspection of Boxes

After you receive your order from New Brunswick Scientific, inspect the boxes carefully
for any damage that may have occurred during shipping. Report any damage to the
carrier and to your local New Brunswick Sales Order Department.

2.2 Unpacking

Save all packing materials and Operating Manual. If any part of your order was damaged
during shipping, missing pieces, or fails to operate properly, contact your New
Brunswick sales representative.

CRUSH WARNING!

Do not attempt to lift the E-25 or E-25R by hand. Always use a lifter or
suitable equipment when raising the shaker.

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2.3 Inspection of Equipment

Verify against your New Brunswick packing list that you have received the correct
materials.

You should have received a Power Kit (power cord, fuse, hex wrench and key) with your
shaker. Make sure your electrical supply matches the electrical specification of the
power kit and the shaker (both of which should be the same).

NOTE:

Use of the Excella E-25/25R Shakers requires a platform, which is a
separate item. See the Available Platforms list in Section 9.2.

New Brunswick Scientific Operating Manual

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2.4 Out of Box Concerns

If any part of your order was damaged during shipping, is missing pieces, or fails to
operate properly, please contact your sales representative.

2.5 Warranty Registration

Please register electronically at our Website: www.nbsc.com

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

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33

3.1 Physical Location

It is essential that the instrument be situated in a area where there is sufficient space for
the shaker and platform to clear walls and obstructions during operation. The surface on
which the shaker is placed must be smooth, level, and able to support the shaker under
full load operating conditions.

CRUSH WARNING!

Do not attempt to lift the E-25 or E-25R by hand. Always use a lifter or
suitable equipment when raising the shaker.

The feet can be adjusted for necessary leveling. Loosen the locking nuts on the threaded
studs attached to the feet of the shaker. Retighten when you have achieved the correct
level for your shaker.

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3.2 Environment

The shaker is designed to operate optimally in the following ambient conditions:

 10 - 35°C
 20 to 80% Relative Humidity non-condensing

3.3 Electrical Requirements

The E-25 shakers can be equipped to run on:

 100 Volts, 50/60 Hz, 800 VA maximum
 120 Volts, 60 Hz, 800 VA maximum
 230 Volts, 50 Hz, 800 VA maximum

The E-25R shakers can be equipped to run on:

 100 Volts, 50/60 Hz, 1500 VA maximum
 120 Volts, 60 Hz, 1500 VA maximum
 230 Volts, 50 Hz, 1500 VA maximum

New Brunswick Scientific Operating Manual

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In all cases, voltage variation must not exceed ± 10%.

3.4 Space Requirements

Figure 1: Space Requirements

1

2

3

4

5

1 Height with lid open: 165.7 cm (65¼ in)
2 10 cm (4 in) front & left side clearance
3 127 cm (50 in)
4 87.6 cm (34½ in) front to back clearance
5 61 cm (24 in) for access to service door

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

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4.1 Front Panel

The keypad on the top (front) panel is the primary user interface to command the Excella
E-10. This section will acquaint you with the keypad’s user interface keys, LED display,
status indicators and function indicators.

Figure 2: Front Panel

44

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2

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Figure 2a: Keypad

3

1 Keypad 4 LED display
2 ON/OFF switch 5 Function indicators
3 Status indicators 6 User interface keys

4

5

6

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4.1.1 LED Display

The digital display on the control panel is a three-digit LED DISPLAY. During
normal shaker operation, the display will indicate:

 Shaker status (On/Off)
 Shaking speed
 Chamber temperature
 Setpoints
 Hours remaining (in a timed run)
 Lid open (“LID”)

4.1.2 User Interface Keys

As you can see in Figure 2a, the keypad has four user interface keys:

START/STOP, ,

 and SELECT. This is how they are used:

 START/STOP

This key is used to start or stop the shaker. It will
also activate or stop the timer when a timed run is
desired.

 (UP), (DOWN)

These keys are used to adjust the setpoint of a
displayed parameter up or down. They also allow
the user to enter the SET MODE for setpoint
changes.

 SELECT

This key is used to change the displayed parameter.

4.1.3 Status Indicators

Four status indicator lights are located to the left of the LED DISPLAY. They are:

 SET

Indicates that the shaker is in the SET MODE, when setpoints are
being displayed and can be altered. This is activated by the

SELECT key or by pressing the  (UP) or  (DOWN) KEY.



POWER

Illuminates and blinks during power up or if power is
interrupted during a run. Press the SELECT key and change to
another function to turn off this indicator.

…continued…

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 HEAT

Illuminates to indicate that the heater is on.

 TIME

Indicates that the timer is in operation. The shaker can be
programmed to run for a preset time from 0.1 to 99.9 hours.
The timer can be disengaged without stopping an ongoing run.

4.1.4 Function Indicators

Four function indicator lights are located to the right of the LED DISPLAY. They
indicate the current parameter(s) being displayed:

 C

Interior chamber temperature. Can be set from 4°C to 60°C,
when in
indexes at 0.1°C increments unless the key is pressed for 4
seconds, after which it indexes more rapidly.



RPM

Revolutions per minute. When in SET MODE, use the  (UP) or
 (DOWN) KEY to change the speed. It indexes at 1 RPM

increments unless the key is pressed for 4 seconds, after which
it indexes more rapidly.

 HRS

Time remaining in a timed run. Can be set from 0.1 to 99.9
hours, in 0.1 increments or, if the  (UP) or  (DOWN) KEY is
pressed for 4 seconds, after which it indexes more rapidly.

The countdown begins when the START/STOP key is pressed.
If the START/STOP key is pressed again, the shaking stops (but
temperature is maintained) and the timer pauses until the

START/STOP key is pressed again.

When a timed run ends, the

SELECT key and change to another function to turn off this

indicator.



MUTE

This feature is activated by the SELECT key. When activated,
the audible alarm is muted, and remains so until it is reactivated
with the
turned off using the
when the machine is powered up again. To activate (or
deactivate) the

MUTE indicator illuminates (or goes out), then press
START/STOP key.

SET MODE, using the  (UP) or  (DOWN) KEY. It

HRS indicator will blink. Press the

SELECT key. If MUTE is activated when the shaker is

ON/OFF switch, it will remain engaged

MUTE function, press the SELECT key until the

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5.1 Installation of Platform

A platform is a separate item that is required for operation. The E-25 and E-25R can
be used with a wide variety of New Brunswick 46 x 76 cm (18 x 30 in) platforms, which
will accept a variety of clamps for flasks test tubes, etc. See Section 9, Replacement
Parts & Accessories, for details.

A platform must be installed on the shaker prior to use.
The shaker is shipped with the four Allen (hexagonal, countersunk) head platform screws

installed in the subplatform of the bearing housing. These screws must be removed
before installing a platform. The Allen (“hex”) wrench is provided.

1. Using the 7/32-inch hex wrench provided, remove and set aside the four Allen head

platform screws from the upper bearing housing (see Figure 3).

Figure 3: Platform Screw Locations

55

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1 Platform screws

2. Place the platform on the upper bearing housing, aligning the mounting holes of the

platform with the platform screw locations.

3. Insert the four platform screws previously removed and, using the hex wrench,

tighten them to secure platform.

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

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5.2 Installing Flask Clamps

Flask clamps purchased for use with universal platforms (see Section 9.2) require
installation. Clamps are installed by securing the base of the clamp to the platform with
the correct type and number of screws. All clamps are shipped complete with hardware.

Clamps for 2-, 2.8- and 4-liter flasks are shipped with an additional girdle to keep the
flasks in place. The girdle is an assembly of springs and sections of rubber tubing. One
girdle is already in place on the clamp, the other is packed separately. To install these
double girdle clamps:

1. Place the clamp on the platform, aligning its mounting holes with holes on the

platform. Secure the clamp in place using the flat Phillips head screws provided. Use

Figure 4b and Table 1 on the following page to help you identify the proper screws,
as three different types of screws are shipped with the clamps.

2. With the first girdle in place, as delivered, on the upper part of the clamp body (see

Figure 4a), insert an empty flask into the clamp.

3. After making sure the sections of tubing are located between the clamp legs, roll the

first girdle down the legs of the clamp as far as it can go. The tubing sections will
rest against the platform, and the springs will be under the clamp base.

4. Place the second girdle around the upper portion of clamp body (just as the first

girdle was initially). Make sure that its spring sections rest against the clamp legs,
while its rubber tubing sections sit against the flask, in between the clamp legs.

Figure 4a: Double Girdle Clamp Installation

2

1

5

3

4

1 Platform 4 Clamp body (legs & base)
2 Clamp mounting holes (5) 5 Lower girdle with girdle tubes
3 Upper girdle with girdle tubes

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Figure 4b: Clamp Fastener



NOTE:

The upper girdle secures the flask within the clamp, and the bottom girdle
keeps the flask from spinning.

New Brunswick flask clamps are used on a variety of shaker platforms. Flat head screws
of different lengths and thread pitch are used to secure the clamp, and all types are
provided when you purchase clamps. To identify the proper screw for your shaker
application by reference to the head style, consult Table 1 below, find the proper screws
and set the others aside:

Table 1: Clamp Hardware Application Chart

No matter what size the clamp, use these screws to fasten them to your platform:

Description Part Number Qty. Application

10-24 x 7.9 mm (5/16 in)
flat Phillips (+) head
screw

S2116-3051 1 7.9 mm (5/16 in) thick

aluminum, phenolic and
stainless steel
platforms.

5.3 Electrical Connections

Before making electrical connections, verify that:

 the power source voltage matches the voltage on the ELECTRICAL SPECIFICATION

PLATE, located on the rear panel (see Figure 5 on the following page)

 the

ON/OFF SWITCH, located on the right side panel as you face the front of the

shaker (see Figure 2), is in the

Connect the

POWER CORD to the POWER CONNECTOR on the rear panel (see Figure 5)

and the other end to a suitable, grounded receptacle.

OFF position.

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Figure 5: E-25 and E-25R Rear Panel

1

2

1 Power connector 2 Electrical specification plate

New Brunswick Scientific Operating Manual

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6.1 Starting the Shaker

To initially start the shaker, close the lid and turn the ON/OFF SWITCH on the right side
panel (see Figure 2, repeated here for easy reference) to the ON position.

66

Figure 2: Front Panel

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1 Keypad & LED display 2 ON/OFF switch

The shaking action may be stopped or started by pressing the START/STOP KEY. When
the shaker begins to operate, the
last entered setpoint.

NOTE:

The shaker will not operate if the lid is open. This is indicated by the word
“LID” appearing in the LED DISPLAY.

6.2 Continuous (Unlimited) Run

1. Press

2. If the display indicates that the shaker is

3. Press either the  (UP) or  (DOWN) KEY to enter SET MODE (the SET INDICATOR

SELECT until the RPM INDICATOR is illuminated.

will illuminate).

LED DISPLAY will track the speed as it accelerates to the

OFF, press the START/STOP KEY.

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4. Set the speed by using the  (UP) or  (DOWN) KEY until the desired setpoint is

displayed. Holding the  (UP) or  (DOWN) KEY will cause the setting to change
more rapidly.

NOTE:

The setpoint may be changed during a run without stopping the shaker by
following steps 2 - 4 above. During speed changes, a visual alarm (flashing

RPM INDICATOR) will flash and an audible alarm will sound until the speed

returns to within 5 rpm of the setpoint.

6.3 Checking a Setpoint

1. Press SELECT until the desired indicator is illuminated.

2. Press either  (UP) or  (DOWN) KEY to enter the SET MODE and display the current

setpoint.

CAUTION!

Holding the  (UP) or  (DOWN) KEY for more than 0.5 seconds causes the
speed setpoint to change. Should this occur, resetting will be necessary.

6.4 Timed Functions

The shaker may be programmed to automatically stop after a preset time period of 0.1 to

99.9 hours. There must be power to the shaker in order to set the timer, although a timed
run can be initiated while the shaker is either stopped or operating.

To set the timer:

1. Press the

2. Press 

between 0.1 and 99.9 hours.

If the shaker is stopped, skip to Step 5 below. If the shaker is already running:

3. Press the

4. Press the START/STOP KEY again; the TIME INDICATOR will light and the shaker will

start the timed run.

SELECT KEY until the HRS INDICATOR is illuminated.

(UP) or  (DOWN) KEY to enter the SET MODE and set the desired run time,

START/STOP KEY. The shaker will stop and the display will read OFF.

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If the shaker is stopped:

5. Press the START/STOP KEY. The shaker will start in untimed mode.

6. Press the START/STOP KEY again. The shaker will stop and the display will read

OFF.

7. Press the START/STOP KEY a third time; the TIME INDICATOR will light and the

shaker will start the timed run.

To disable the visual alarm (flashing TIME INDICATOR), press the SELECT KEY and
change to any other function.

To cancel the timer while the shaker is running:

1. Press the SELECT KEY until the HRS indicator lights.

2. Press the  KEY until 0.0 is displayed, then press the START/STOP KEY. The display

will read OFF, the shaker will stop, and the TIME INDICATOR light will turn off.

3. Press the START/STOP KEY to continue in untimed mode.

To cancel the timer while the shaker is stopped:

1. Press the  KEY until 0.0 is displayed, then press the START/STOP KEY. The TIME

INDICATOR

will light and the shaker will run.

2. Press the START/STOP KEY. The shaker will stop and the TIME INDICATOR will turn

off.

3. Press the START/STOP KEY a third time, and the shaker will run in untimed mode.

6.5 Alarm Functions

In addition to the visual alarm mentioned above, the Excella E-25 and E-25R shakers
have an audible alarm that is activated at predetermined times. It can be deactivated by
using the

MUTE function:

1. Press the

2. Press the 

SELECT key until the MUTE indicator illuminates.

(UP) or  (DOWN) KEY to display ON, then press the SELECT KEY.

To reactivate the audible alarm:

1. Press the SELECT key until the MUTE indicator illuminates.

2. Press the  (UP) or  (DOWN) KEY to display OFF, then press the SELECT KEY.

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6.6 Temperature Setpoint

Press the SELECT KEY until the function C INDICATOR illuminates. The temperature can
be set from 5C above ambient temperature to 60C (non-refrigerated shakers) or from
4C to 60C (refrigerated shakers). Increasing or decreasing the setpoint is accomplished
with the  (UP) or  (DOWN) KEY. Ambient temperature is measured one meter from
the front of the shaker.

During operation, if the temperature of the chamber is more than 1.0C higher or lower
than the temperature setpoint, an alarm is triggered. This alarm consists of a flashing C

INDICATOR

and audible beep. The alarm will automatically deactivate as the shaker

achieves the set temperature.

6.7 Temperature Offset Calibration

The temperature probe and the temperature controller are calibrated together at the
factory. The temperature probe measures the temperature of the air at the probe’s
location, near the heat exchanger return vent. The controller uses the probe input to
adjust air temperature, up or down, to match the temperature setpoint.

Depending on various conditions within the chamber, such as flask placement and size,
the heat produced by growing organisms, heat losses due to liquid evaporation from
flasks, etc., the display temperature may differ from temperatures within the flasks
themselves.

If you wish to have the temperature display (“Indicated Temperature”) match the
temperature at a given point, or match the average of a series of points within the
chamber (“Actual Temperature”), proceed as follows:

1. Let the shaker equilibrate at or near the desired temperature. Record the Indicated

Temperature.

2. Record the Actual Temperature.

3. Calculate the temperature correction value: Actual Temperature – Indicated

Temperature = Temperature Correction Value.

4. Press the SELECT KEY until the function C INDICATOR illuminates.

5. Simultaneously press the 

CAL.

6. Using the 

(UP) or  (DOWN) KEY, enter the Temperature Correction Value

(UP) and  (DOWN) KEYS. The display will indicate

calculated in Step 3 above.

7. Simultaneously press the 

(UP) and  (DOWN) KEYS to save the Temperature

Correction Value to memory.

New Brunswick Scientific Operating Manual

26

NOTE:

°C light will pulse rapidly to indicate it is not operating in the factory

The
default mode. It will pulse for a longer duration and less rapidly (with a
frequency of approximately one second) to indicate temperature is more
than one degree above or below setpoint.

To return to the factory calibration:

1. Press the

2. Simultaneously press the  (UP) and  (DOWN) KEYS. The display will indicate

CAL.

3. Using the 

4. Simultaneously press the  (UP) and  (DOWN) KEYS. The rapid pulsing of the C

INDICATOR will stop.

6.8 Power Failure

SELECT KEY until the function C INDICATOR illuminates.

(UP) or  (DOWN) KEY, set the Temperature Correction Value to zero.

In the event of a power failure, the E-25 & E25R shakers are equipped with an
automatic restart function.

If the shaker was in operation prior to the power interruption, when power is restored the
shaker will begin to operate at its last entered setpoint. The
the audible alarm will sound, indicating that a power failure has occurred. Press any key
to cease the flashing in the display and the audible alarm.

6.9 Speed Calibration

To calibrate the shaking speed:

1. Set the shaker to a speed that can easily be measured. If you are using a strobe,

minimum speed should be 250 RPM.

2. Compare the reading on the display to the measured reading.

If an adjustment is needed:

1. Press the

SELECT KEY until the RPM indicator light illuminates.

2. Press the  (UP) and  (DOWN) KEYS simultaneously. The display will indicate

CAL.

3. Press either the  (UP) or  (DOWN) KEY to change the displayed value to match the

measured speed.

4. Press the 

5. Turn the shaker

(UP) and  (DOWN) KEYS simultaneously to save the adjustment.

OFF using the power switch, then turn it back ON.

LED DISPLAY will flash and

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

27

6.10 Maximum Recommended Speed

Figure 6 shows the maximum recommended speed for your shaker, according to load.

Figure 6: Load & Speed for E-25/E-25R

400

350

300

250

200

150

100

50

0

<30 30 to 36 36 to 40 40 to 60 >60

50,

12 5 ,
250 m L
20% Full

Pla tform and Load (lb)

1L ,
2L,

2.8L

20% Full

NOTE:

In Figure 6, “20% Full” refers to the amount of liquid in the flasks. The
platforms are fully loaded with flasks.

New Brunswick Scientific Operating Manual

28

77

WARNING!

Always turn off the shaker and disconnect the power cord from the power
supply BEFORE performing any maintenance on the shaker.

7.1 Cleaning External Surfaces

The shaker may be cleaned using a damp cloth or any standard, household or laboratory
cleaner to wipe down its outer surfaces. Do not use abrasive or corrosive compounds to
clean this instrument, as they may damage the shaker and void the warranty.

7.2 Fuse Replacement

Excella E-25 models require one electrical fuse, which is housed in the fuse holder
located on the M1324-7004 PCB (see Figure 8a). E-25R models have two electrical
fuses, housed in the two fuse holders that are located on the M1324-7003 PCB (see
Figure 8b).

To check or replace a fuse:

1. Set the ON/OFF SWITCH to Off and disconnect the POWER CORD from the power

source.

2. Remove the top right bezel from the shaker: with one hand, grasp the top edge, and

with the other, using the cut-out provided on the bottom, grasp the bottom edge. Pull
the bezel up from the bottom, then out and away. Set it aside.

3. With reference to Figure 7, which looking down at the top of the shaker, remove the

two screws that fasten the electrical panel in place. Set the screws aside for reuse.

Figure 7: Top Right Bezel Removed

P

RREEVVEENNTTIIVVEE

P

M

AAIINNTTEENNAANNCCE

M

1

E

2

1 Remove these screws 2 Electrical panel

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

29

4. Swing the ELECTRICAL PANEL to the right to access the PCB board that houses the

fuse(s); see Figures 8a (E-25) and 8b (E-25R), where Item 1 identifies fuse holders—
see Figures 8c & 8d for details.

Figure 8a: Rear of Electrical Panel (E-25)

1

Figure 8b: Rear of Electrical Panel (E-25R)

1

New Brunswick Scientific Operating Manual

30

Figure 8c: Fuse Holder Detail (E-25)

1

Figure 8d: Fuse Holder Detail (E-25R)

1

1 Fuse

5. Remove the fuse and check it. If it has failed, replace the fuse.

6. Swing the ELECTRICAL PANEL to the left, flush against the shaker.

7. Using the screws set aside, fasten the ELECTRICAL PANEL in place.

8. With two hands, reinstall the top bezel, snapping the back in place first, then the

front. Verify that it fits snugly and securely.

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

31

WARNING!

The following procedures are provided for your information only.
Do not attempt to perform these service interventions yourself unless you
are an authorized service technician.

7.3 Belt Replacement

To gain access to the drive belt, your service technician will follow these steps with
reference to Figure 9:

WARNING!

Always keep fingers clear of the drive belt and pulley.

1. Set the ON/OFF power switch to OFF.

2. Disconnect the power cord from the electrical outlet.

3. Open the lid.

4. Using an Allen wrench, remove the four Allen head screws that hold the platform to

the bearing housing. Set the platform and its mounting screws aside for reuse.

5. Remove the E-ring that holds the upper bearing housing to the shaft.

6. Remove the four nuts (two on either side) from the bearing housing mounting plate.

These nuts hold the flexure arms in place.

7. Slide the upper bearing housing off the shaft, and set it aside.

8. Use the hex wrench to loosen the four hex nuts on the motor mounting bracket.

9. Gently slide the motor mounting bracket toward the righthand side of the shaker.

This loosens the drive belt from the motor pulley and the large counterweighted
pulley. Moving the motor mounting bracket forward will cause the belt to fall from
both belt tracks.

10. Remove the old belt.

11. With one hand, place the new belt around the motor pulley, and with the other hand

guide the belt around the large counterweighted pulley.

12. Move the motor mounting bracket back, until there is a slight resistance.

13. Verify that the drive belt has a slight pressure near the center. The recommended

deflection is 9.5 mm (3/8 in).

New Brunswick Scientific Operating Manual

32

Figure 9: Drive Belt Replacement

2

1

4

3

4

5

8

6

7

1 Upper bearing housing 5 Counterweighted pulley
2 E-ring 6 Drive belt
3 Flexure arm 7 Motor mounting bracket
4 Flexure arm nuts 8 Motor pulley

14. Use the hex wrench to tighten the four hex nuts

on the motor mounting bracket.

15. Reinstall the upper bearing housing on the shaft.

16. Reattach the flexure arms to the bearing housing mounting plate with the four nuts

previously removed.

17. Reattach the E-ring to the shaft.

18. Reinstall the platform on the bearing housing with the four Allen head screws

previously removed.

19. Close the shaker lid.

20. Reconnect the power cord to the electrical outlet.

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

33

88

If any problems occur with your shaker, do not attempt to perform any service on the shaker
other than specified in this manual. Unauthorized servicing may void the warranty. Please

contact your local New Brunswick Sales Order Department

In any correspondence with New Brunswick, please refer to the Model Number and Serial
Number of your shaker. This information is on the ELECTRICAL SPECIFICATION PLATE,
located on the rear panel of the shaker.

There are some problems, however, that you can investigate and correct yourself. Refer to the
following Troubleshooting Guide:

Symptom(s) Probable Cause(s) & Solution(s)

No power; display is not on; power cord is not plugged in and/or
power switch is off: plug in power cord (to working electric
outlet), and turn on power switch.
Door is open: close door firmly, making sure latch is engaged.
Door is closed but not completely: door magnet is not adjusted
correctly; call for service.
On/Off switch is not working: call for service.
Fuse(s) burned out: check and replace as needed.

Shaker does not run.

Shaker runs slowly
and/or no speed
indication.

Shaker does not run at
set speed.

If you recently replaced a fuse, it may not have been seated
properly: remove and reinstall the fuse carefully.
Shaking speed has been set to Zero by program running or by
computer interface: reset shaking speed.
Defective main board: call for service
Defective display controller board: call for service.
Jammed shaking mechanism: check for debris; if necessary,
call for service
Defective motor: call for service
Drive belt out of alignment or worn: call for service.
If you recently replaced a fuse, it may not have been seated
properly: remove and reinstall the fuse carefully.
Incorrect speed calibration: recalibrate shaking speed.
Defective main board: call for service.
Defective tach board: call for service
Defective motor: call for service.
Drive belt is out of alignment or worn: call for service.
Shaker is overloaded and/or you are using baffled flasks:
remove some contents & balance load.
Defective motor: call for service.
Drive belt out of alignment or worn: call for service.

...continued...

T

RROOUUBBLLEESSHHOOOOTTIINNG

T

G

New Brunswick Scientific Operating Manual

34

Symptom(s) Probable Cause(s) & Solution(s)

Load out of balance: unload all contents, then reload.

Operating noise

Incubator does not
reach set temperature.

indication.

Loose component(s) in platform, subplatform and/or drive
assembly: call for service.
Heater fuse blown: replace.
Compressor fuse blown: replace.
Compressor over-pressure switch activated: call for service.
Ambient temperature too high or too low: cool or heat the room
as needed.
Defective heater: call for service.
Defective refrigeration system: call for service.
Incorrect temperature indication (see below).
Defective RTD assembly: call for service. Incorrect temperature
Defective main board: call for service.

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

35

99

When ordering replacement or accessory parts, or requesting service information, please provide
the Model Number and Serial Number of your shaker. This information is on the ELECTRICAL

SPECIFICATION PLATE

9.1 Replacement Parts

8.0A Fuse (Motor) 1 (E-25)
15A Circuit Breaker 1 P0400-4305

120V 15A Power Cord 1 P0720-2024
220V Power Cord 1 P0720-2021
V Belt 1 P0700-7070
Gasket, Door 1 M1353-9900
AC Connector, Power Entry 1 P0460-2205
Drive Assembly 1 M1353-1003

, located on the side panel of the shaker.

Table 2: Service Parts

Part Description Qty Req’d Part Number

R

EEPPLLAACCEEMMEENNTT

R

A

A

2 (E-25R)

CCCCEESSSSOORRIIEES

P

AARRTTSS

P

P0380-3790

&

&

S

9.2 Accessories

Platform Description Capacity Part Number

See Table 4 on the following page M1250-9920

50 ml Erlenmeyer Flasks 108 M1191-9908
125 ml Erlenmeyer Flasks 60 M1191-9909
250 ml Erlenmeyer Flasks 40 M1191-9910
500 ml Erlenmeyer Flasks 24 M1191-9911
1 L Erlenmeyer Flasks 15 AG-1
2 L Erlenmeyer Flasks 12 AG-2

2.8 L Fernbach Flasks 6 AG-28
4 L Erlenmeyer Flasks 6 AG-4
6 L Erlenmeyer Flasks 4 AG-6

Table 3: Platforms

Universal Platform

Dedicated Platforms

New Brunswick Scientific Operating Manual

36

Table 4: Universal Platform Flask Capacity

Flask Type Capacity

50 ml Erlenmeyer Flasks 92
125 ml Erlenmeyer Flasks 39
250 ml Erlenmeyer Flasks 30
500 ml Erlenmeyer Flasks 18
1 L Erlenmeyer Flasks 12
2 L Erlenmeyer Flasks 8

2.8 L Fernbach Flasks 6
4 L Erlenmeyer Flasks 6
6 L Erlenmeyer Flasks 4

Table 5: Carriers & Test Tube Racks

Accessory Description Part Number

Utility Tray with rubber mat for shaking 96 well plates, petri
dishes and other labware at low speeds.
Angled Test Tube Rack Holder for user-supplied test tube
racks that are 10 - 13 mm (4 - 5 in) wide and up to 38 mm
(15 in) long. Capacity: 4 racks/platform.
Angled Test Tube Rack Spacer for use with TTR-210 to
accommodate test tube racks that are less than 13 mm
(5 in) wide and up to 38 mm (15 in) long.
Microtiter Plate Carrier, capacity up to 4 microtiter plates TTR-221*
80-tube (8-11mm) Adjustable Angle Test Tube Rack M1289-0100
60-tube (12-15mm) Adjustable Angle Test Tube Rack M1289-0200
42-tube (15-18mm) Adjustable Angle Test Tube Rack M1289-0300
30-tube (18-21mm) Adjustable Angle Test Tube Rack M1289-0400
22-tube (22-26mm) Adjustable Angle Test Tube Rack M1289-0500
20-tube (26-30mm) Adjustable Angle Test Tube Rack M1289-0600
Microplate Holder, stack 3 deep-well or 9 standard microplates M1289-0700

*Universal Platform Required

 Platform capacity is 7 racks
 Platform capacity is 16 racks

AG-00

TTR-210*

TTR-215*

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

37

1100

Figure 10: E-25 & E-25R Control Schematic (Overview)

D

RRAAWWIINNGGS

D

S

NOTE:

For a larger version of this drawing, contact your sales or service
representative.

New Brunswick Scientific Operating Manual

38

Figure 11a: 230V ac, 50 Hz Power Schematic

230Vac/50Hz OPERATION

POWER

TRANSFORMER

M1324-8015

N

230V

100V

120V

M1324-7003

RED
ORANGE
BLACK
WHITE
YELLOW

VIOLET

FROM

J4-1
J4-3

J4-5
J4-2
J4-4
J4-6

COND. FAN

OVER PRESSURE

COMPRESSOR

UNIT

M1324-8017

SWITCH

WHITE

M1324-8016

M1324-8017

RED

GRAY

BLACK

TB001

Figure 11b: 100V ac, 60 Hz Power Schematic

100Vac/60Hz OPERATION

POWER

TRANSFORMER

M1324-8015

N

230V

100V

120V

M1324-7003

ORANGE
BLACK
RED
WHITE
YELLOW

VIOLET

FROM

J4-3
J4-5
J4-1

J4-2
J4-4
J4-6

COND. FAN

OVER PRESSURE

COMPRESSOR

UNIT

M1324-8017

SWITCH

WHITE

M1324-8016

M1324-8017

RED

GRAY

BLACK

TB001

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

39

Figure 11c: 120V ac, 60 Hz Power Schematic

120Vac/60Hz OPERATION

FROM

M1324-7003

RED
ORANGE
BLACK
WHITE
YELLOW

VIOLET

J4-1
J4-3

J4-5
J4-2
J4-4
J4-6

COND. FA N

OVER PRES SURE

COMPRESSOR

UNIT

TB001

M1324-8017

M1324-8016

SWITCH

M1324-8017

Figure 11d: 100V ac, 50 Hz Power Schematic

100Vac/50Hz OPERATION

POWER

TRANSFORMER

M1324-8015

COND. FAN

OVER PRESSURE

COMPRESSOR

UNIT

M1324-8017

SWITCH

N

WHITE

M1324-8016

M1324-8017

100V

GRAY

120V

BLACK

230V

RED

TB001

M1324-7003

ORANGE
RED
BLACK
WHITE
YELLOW
VIOLET

FROM

J4-3
J4-1
J4-5

J4-2
J4-4
J4-6

New Brunswick Scientific Operating Manual

40

Figure 12: E-25R Refrigeration Schematic

10.1 List of Drawings

Figure Description Page

1 Space Requirements 14
2 Front Panel 15, 22

2a Keypad 15

3 Platform Screw Locations 18
4a Double Girdle Clamp Installation 19
4b Clamp Fastener 20

5 E-25 and E-25R Rear Panel 21

6 Load & Speed For E-25/E-25R 27

7 Top Right Bezel Removed 28
8a Rear of Electrical Panel (E-25) 29
8b Rear of Electrical Panel (E-25R) 29
8c Fuse Holder Detail (E-25) 30
8d Fuse Holder Detail (E-25R) 30

9 Drive Belt Replacement 32
10 E-25 & E-25R Control Schematic (overview) 37

11a 230Vac, 50 Hz Power Schematic 38
11b 100Vac, 60 Hz Power Schematic 38
11c 120Vac, 60 Hz Power Schematic 39
11d 100Vac, 50 Hz Power Schematic 39

12 E-25R Refrigeration Schematic 40

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

41

10.2 List of Tables

Table Description Page

1 Clamp Hardware Application Chart 20

2 Service Parts 35

3 Platforms 35

4 Universal Platform Flask Capacity 36

5 Carriers & Test Tube Racks 36

-- List of Drawings 40

-- List of Tables 41

New Brunswick Scientific Operating Manual

42

A

Accessories · 35
Actual Temperature · 25
Alarm Functions · 24
Ambient Temperature · 7
Audible Alarm

Disabling · 24

C

Calibrating Speed · 26
Carriers · 36
CE Standards · 10
Certifications · 9
Checking Setpoints · 23
Clamp Hardware · 20
Cleaning External Surfaces · 28

Contact New Brunswick

How to · 2

Continuous Run · 23
Copyright Notice · 3
Customer Satisfaction Form 6300 · 12

D

1111

Replacement · 28

Fuse Holders · 21

I

Incomplete Order · 12
Indicated Temperature · 25
Inspection · 11
Installation

Platform · 18

Installing Flask Clamps · 19

K

Keypad Location · 22

L

LED Display · 16
Lifting the Shaker · 13
Load & Speed Graph · 27

M

Maximum Recommended Speed · 27
Missing Parts · 12

I

NNDDEEX

I

X

Declaration of Conformity · 10
Dedicated Platforms · 35
Disclaimer Notice · 3
Double Girdle Clamps · 19
Drawings

Index of · 40

E

Electrical Connections · 20
Environment · 13
Extended Warranty Option · 4

F

Features

Status Indicators · 16

Flask Capacity

Universal Platform · 36

Flask Clamps

Installation of · 19
Front Panel · 22
Function Indicators · 17
Fuse

N

New Brunswick Offices · 2

O

On/Off Switch Location · 22
Operation · 22
Overview · 7

P

Platform

Installation · 18
Platforms · 35
Power Connector · 21
Power Failure · 26
Power Kit · 11
Problems · 33

R

Rear Panel · 21

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

43

Regulatory Standards · 9
Relative Humidity · 13
Replacement Parts · 35

S

Service Parts · 35
Shaking Speed

Maximum Recommended · 27
Shipping Damage · 12
Specifications · 8
Speed Calibration · 26
Starting the Shaker · 22
Status Indicators · 16

T

Table of Contents · 5
Tables

Index of · 41
Temperature Correction Value · 25
Temperature Offset Calibration · 25
Temperature Setpoint · 25
Test Tube Racks · 36

Timed Functions · 23
Timer

Cancelling the · 24

Troubleshooting · 33

U

Universal Platform · 35
Unlimited Run · 23
Unpacking · 11
User Interface Keys · 16

V

Visual Alarm

Disabling · 24

W

Warranty · 4

Option for Extended · 4

Warranty Registration · 4, 12

New Brunswick Scientific Operating Manual

44

M1353-0050  Excella® E-25/E25R Classic Incubator Shakers Operating Manual

Your local distributor for New Brunsdwick products: www.nbsc.com/ContactUs

New Brunswick Scientific, 175 Freshwater Boulevard, Enfield, CT 06082-4444 USA

Eppendorf AG  22331 Hamburg  Germany  Tel: [phone]  Fax: [phone]  E-mail: [e-mail]

New Brunswick Scientific Europe B.V.  Nijmegen  The Netherlands  Tel: [phone]  E-mail: [e-mail]

Eppendorf North America, Inc.  Hauppauge, NY  USA Tel: [phone]  [phone]  E-mail: [e-mail]

Application Support Europe, International: Tel: [phone]  E-mail: [e-mail]

North America : Tel : [phone] menu option 2  E-mail: [e-mail]

Asia Pacific: Tel: [phone]  E-mail: [e-mail]