Eppendorf E-24R User Manual
New Brunswick Benchtop Incubator Shakers
Excella® E-24/24R
Operating Manual
M1352-0050
Revision D
2
COPYRIGHT:
Copyright © 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.
The company reserves the right to change information in this document
without notice. Updates to information in this document reflect our commitment to
continuing product development and improvement.
TRADEMARKS:
Eppendorf
Logo™ are trademarks of Eppendorf AG, Hamburg, Germany.
Excella
Germany.
Trademarks are not marked in all cases with ™ or ® in this manual.
Eppendorf has attempted to identify the ownership of all trademarks from public records. Any
omissions or errors are unintentional.
®
is a registered trademark, and New Brunswick™ and the New Brunswick
®
and Innova® are trademarks owned and registered by Eppendorf AG, Hamburg,
April10, 2012
Revision D
M1352-0050
Excella E-24/24R Incubator Shakers M1352-0050 Operating Manual
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CAUTION!
This equipment must be operated as described in this manual. If
operational guidelines are not followed, equipment damage and personal
injury can occur.
Please read the entire operating manual before attempting to use this unit.
Do not use this equipment in a hazardous atmosphere or with hazardous
materials for which the equipment was not designed.
Eppendorf is not responsible for any
damage to this equipment that may result from the use of an accessory
not manufactured by Eppendorf.
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TABLE OF CONTENTS
1 OVERVIEW.......................................................................................................................... 6
2 INSPECTION & UNPACKING OF EQUIPMENT ......................................................... 8
2.1 I
NSPECTION OF BOXES .....................................................................................................8
2.2 PACKING LIST VERIFICATION .......................................................................................... 8
2.3 UNPACKING OF EQUIPMENT............................................................................................. 8
2.4 OUT OF BOX CONCERNS .................................................................................................. 9
2.5 WARRANTY REGISTRATION............................................................................................. 9
3 PREPARING THE LOCATION....................................................................................... 10
3.1 PHYSICAL LOCATION ..................................................................................................... 10
3.2 ENVIRONMENT............................................................................................................... 10
3.3 ELECTRICAL REQUIREMENTS......................................................................................... 10
3.4 SPACE REQUIREMENTS .................................................................................................. 11
4 INSTALLATION................................................................................................................ 12
4.1 INSTALLATION OF PLATFORM ........................................................................................ 12
4.2 FLASK CLAMP INSTALLATION........................................................................................ 13
4.3 ELECTRICAL CONNECTIONS........................................................................................... 14
5 FEATURES......................................................................................................................... 15
5.1 CONTROL PANEL............................................................................................................ 15
5.1.1 Keypad .................................................................................................................. 15
5.1.2 User Interface Keys .............................................................................................. 16
5.1.3 LED Display.......................................................................................................... 16
5.1.4 Status Indicators................................................................................................... 17
5.1.5 Function Indicators............................................................................................... 17
6 OPERATION ...................................................................................................................... 19
6.1 ELECTRICAL CONNECTIONS........................................................................................... 19
6.2 S
TARTING THE SHAKER.................................................................................................. 19
6.3 CONTINUOUS (UNTIMED) RUN....................................................................................... 20
6.4 C
HECKING ANY SETPOINT ............................................................................................. 20
6.5 TIMED FUNCTIONS......................................................................................................... 20
6.6 A
6.7 T
LARM FUNCTIONS........................................................................................................ 21
EMPERATURE SETPOINT............................................................................................... 21
6.8 TEMPERATURE OFFSET CALIBRATION ........................................................................... 22
6.9 P
6.10 S
OWER FAILURE ............................................................................................................ 23
PEED CALIBRATION...................................................................................................... 23
7 MAINTENANCE................................................................................................................ 24
7.1 R
7.2 C
Excella E-24/24R Incubator Shakers M1352-0050 Operating Manual
OUTINE MAINTENANCE ............................................................................................... 24
LEANING EXTERNAL & INTERNAL SURFACES.............................................................. 24
5
7.3 BIOHAZARD DECONTAMINATION................................................................................... 24
8 SERVICE.............................................................................................................................26
8.1 T
8.2 P
ROUBLESHOOTING ....................................................................................................... 26
RODUCT RETURNS........................................................................................................ 27
8.3 OPENING THE SERVICE COMPARTMENT......................................................................... 27
8.4 FUSE REPLACEMENT...................................................................................................... 28
8.5 BELT REPLACEMENT OR ADJUSTMENT .......................................................................... 30
8.6 REPLACEMENT PARTS.................................................................................................... 32
9 ACCESSORIES .................................................................................................................. 33
9.1.1 Platforms............................................................................................................... 33
9.1.2 Flask Clamps for Universal Platforms................................................................. 33
9.1.3 Replacement Clamp Hardware Kits ..................................................................... 34
9.1.4 Test Tube Racks & Other Accessories.................................................................. 34
10 DRAWINGS & TABLES............................................................................................... 36
10.1 SCHEMATICS.................................................................................................................. 36
10.2 LIST OF DRAWINGS........................................................................................................ 39
10.3 LIST OF TABLES ............................................................................................................. 40
11 SPECIFICATIONS......................................................................................................... 41
11.1 CERTIFICATIONS ............................................................................................................ 42
12 APPENDIX: PRODUCT RETURNS...........................................................................44
12.1 RETURN PROCEDURE ..................................................................................................... 44
12.2 RETURN AUTHORIZATION AND DECONTAMINATION CERTIFICATE ................................ 44
13 INDEX.............................................................................................................................. 46
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The Excella® E-24/24R Benchtop Incubator Shakers use a UniCentric™ counter-balanced drive
mechanism. They provide horizontal plane rotary motion in a ¾-inch (1.9 cm) diameter circular
orbit. A Proportional/Integral (PI) microprocessor controls the speed and temperature over the
entire range.
The incubated and refrigerated E-24R operates from 15ºC below ambient (with a minimum
setpoint of 4ºC) to 60C, and the incubated E-24 from 7ºC above ambient to 60ºC. Both these
ranges depend on relative humidity and other ambient factors. Ambient temperature is measured
one meter from the front of the unit.
Erlenmeyer flasks, 2.8-liter Fernbach flasks, and a wide variety of tubes and plates can be
accommodated using the New Brunswick shaker accessories described in Section 9.
The E-24/24R may be operated in the following ways:
Continuously: at a set speed and temperature, until user intervention.
In a timed mode: run at a set speed, time and temperature for a period of up to 99.9
hours, after which the shaker automatically shuts off, while the temperature is maintained
at its setpoint.
For safe operation, the E-24/24R shakers are designed with a safety switch that automatically
stops the shaker mechanism when the lid is opened.
Both the E-24 and the E-24R are equipped with visual and audible alarms that alert the user to
the following conditions:
The end of a timed run
Deviations from speed setpoint (5 minutes after lid is closed)
Deviations from temperature setpoint (5 minutes after lid is closed)
Power failure
Lid open
To accommodate customer needs, a wide variety of platforms can be used with the Excella
E-24/24R:
Universal platforms are the most flexible, providing hole patterns for flask clamps,
test tube racks and other accessories.
Dedicated platforms are supplied with flask clamps attached; they are designed solely
and expressly for this purpose.
O
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W
Excella E-24/24R Incubator Shakers M1352-0050 Operating Manual
7
Test tube racks, microplate holders, and test tube rack holders are also available (a
universal platform is needed for all test tube racks and holders).
For further information on these accessories, see Section 9.
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22
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2.1 Inspection of Boxes
After you receive your order, inspect the boxes carefully for any damage that may have
occurred during shipping. Report any damage immediately to the carrier and to your
local sales representative.
2.2 Packing List Verification
Verify against your packing list that you have received all of the correct materials.
2.3 Unpacking of Equipment
WARNING!
Do not attempt to lift the Excella E-24/24R by yourself. Always ask for
assistance or use a lifter or other suitable equipment when raising or
handling the unit.
CRUSH WARNING!
It is preferable to lift the Excella E-24/24R from the sides of the unit,
but if you lift it from the front and back, be sure to use the finger holes
in the back lip (toward the bottom of the unit). If these finger holes are
not used, the back lip can pinch fingers and hands.
Upon unpacking the unit, inspect it carefully for any damage that may have occurred
during transit. Report any apparent damage to the carrier and to your sales
representative. Save the crate and packing materials.
T
NOTE:
Use of the Excella E-24/24R Shakers requires a platform, which is a separate
item. See the Available Platforms list in Section 9.1.1.
Excella E-24/24R Incubator Shakers M1352-0050 Operating Manual
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2.4 Out of Box Concerns
If any part of your order was damaged during shipping, is missing pieces, or fails to
operate properly, please contact your sales representative and also fill out Customer
Satisfaction Form 6300, included with your equipment, and return it by fax.
2.5 Warranty Registration
Please complete and return your warranty card or register electronically at our Website:
www.nbsc.com
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33
3.1 Physical Location
WARNING!
Do not attempt to lift the Excella E-24/24R by yourself. Always ask for
assistance or use a lifter or other suitable equipment when raising or
handling the unit.
CRUSH WARNING!
It is preferable to lift the Excella E-24/24R from the sides of the unit,
but if you lift it from the front and back, be sure to use the finger holes
in the back lip (toward the bottom of the unit). If these finger holes are
not used, the back lip can pinch fingers and hands.
The surface where you place the E-24/24R should be smooth, level and sturdy, and must
be able to accommodate 200 pounds.
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3.2 Environment
The shaker is designed to operate optimally in the following ambient conditions:
10º to 35°C
20 to 80% Relative Humidity (non-condensing)
3.3 Electrical Requirements
The E-24/24R can be equipped to run on:
100 Volts, 50/60 Hz, 1500 VA maximum
120 Volts, 60 Hz, 1500 VA maximum
230 Volts, 50 Hz, 1500 VA maximum
In all cases, voltage variations must not exceed ±10%.
Excella E-24/24R Incubator Shakers M1352-0050 Operating Manual
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3.4 Space Requirements
It is essential that the shaker be situated in an area where there is sufficient space for the
unit and its service lines (see Figure 1).
Figure 1: Space Requirements
Allow 42 inches of
height to provide
clearance (for
opening the lid)
Allow 27 inches
of width
to provide clearance
Allow 33 inches of depth
to provide clearance
The dimensions of the Excella E-24/24R are:
Width
Depth
Height
Height with lid open
22 inches 55.8 cm
30 inches 76.2 cm
24 1/16 inches 61.1 cm
40½ inches 101.9 cm
NOTE:
Be sure to allow at least three inches (7.6 cm) around shaker for ventilation,
access to power cord (rear panel), and access to power switch and RS-232
port (right side).
The effective surface area required for operation is:
Width
Depth
24 inches 61 cm
30 inches 76.2 cm
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4.1 Installation of Platform
44
I
NNSSTTAALLLLAATTIIOON
I
NOTE:
There are two small plastic straps that hold the bearing housing in place for
shipping. The straps must be removed from the unit.
Prior to use, a platform must be installed on the unit. The unit is shipped with four
Allen head PLATFORM SCREWS installed in the SUBPLATFORM of the BEARING
HOUSING
installed:
. These screws must be removed and set aside for use before a platform can be
Figure 2: Platform Screw Locations
N
SUBPLATFORM
1. Using the 7/32-inch hex wrench provided, remove the four Allen head platform
screws from the subplatform. Set them aside.
2. Place the selected
platform with the platform screw locations in the subplatform.
3. Insert the four Allen head platform screws previously removed and set aside. Tighten
them with the 7/32-inch hex wrench provided to secure the platform.
Excella E-24/24R Incubator Shakers M1352-0050 Operating Manual
PLATFORM on the subplatform. Align the mounting holes of the
13
4.2 Flask Clamp Installation
Flask clamps purchased for use with universal platforms (see Section 9.1.1) require
installation. Clamps are installed by securing the base of the clamp to the platform with
the correct type and number of screws. All clamps are shipped complete with hardware.
NOTE:
The Excella E-24 and E-24R platforms require 10-24 x 5/16-inch screws,
which are the smaller Phillips head screws supplied, to fasten flask clamps.
Clamps for 2- and 2.8-liter flasks are shipped with an additional girdle to keep the flasks
in place. The girdle is an assembly of springs and sections of rubber tubing. One girdle
is already in place on the clamp, the other is packed separately. To install these double
girdle clamps:
1. Place the clamp on the platform, aligning its mounting holes with holes on the
platform. Secure the clamp in place using the flat Phillips head screws provided
(#S2116-3051, 10-24 x 5/16-inch). Use Figure 3b to help you identify the proper
screws, as three different types of screws are shipped with the clamps.
2. With the first girdle in place, as delivered, on the upper part of the clamp body (see
Figure 3a), insert an empty flask into the clamp.
3. After making sure the sections of tubing are located between the clamp legs, roll the
first girdle down the legs of the clamp as far as it can go. The tubing sections will
rest against the platform, and the springs will be under the clamp base.
4. Place the second girdle around the upper portion of clamp body (just as the first
girdle was initially). Make sure that its spring sections rest against the clamp legs,
while its rubber tubing sections sit against the flask, in between the clamp legs.
Figure 3a: Double Girdle Clamp Installation
UPPER GIRDLE
WITH GIRDLE
TUBES
PLATFORM
CLAMP
MOUNTING
HOLES (5)
LOWER
GIRDLE WITH
GIRDLE TUBES
CLAMP BODY
(LEGS AND BASE)
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Figure 3b: Clamp Fastener
NOTE:
The upper girdle secures the flask within the clamp, and the bottom girdle
keeps the flask from spinning.
New Brunswick flask clamps are used on a variety of shaker platforms. Flat head
screws of different lengths and thread pitch are used to secure the clamp. With
reference to Figure 3b above, select the appropriate screws and set the others aside.
NOTE:
One-liter and larger flask clamps are fastened with 5 screws.
4.3 Electrical Connections
CAUTION!
Before making electrical connections, be sure to check the following:
1. If you have not already done so, check that the voltage and frequency of your unit are
compatible with your electric supply.
2. Remove the caution label from the rear of the unit.
3. Set the circuit breaker on the right side of the unit to the OFF position.
ONLY THEN:
4. Plug the power cord into a grounded electrical outlet.
CAUTION!
A grounded electrical outlet is necessary for the safe operation of this
instrument.
Excella E-24/24R Incubator Shakers M1352-0050 Operating Manual
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5.1 Control Panel
The front panel (see Figure 4) of the Excella E-24/24R shakers provides easy access to
the control panel, which consists of the keypad, display and indicator lights. The ON/OFF
switch is conveniently located on the right side of the unit, as you face the controls.
Figure 4: Control Panel
INDICATORS (see Sections 5.1.4 & 5.1.5)
55
F
EEAATTUURREES
F
LED DISPLAY
(see Section 5.1.3 )
S
ON/OFF
SWITCH
KEYPAD (see Sections 5.1.1 & 5.1.2)
5.1.1 Keypad
The keypad (see close-up in Figure 4a on the following page) is the operator’s
command center for the shaker. In one convenient location are grouped the user
interface keys, the LED display, the status indicator lights and the function
indicator lights.
New Brunswick Operating Manual
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