o
r usees and Microplates
N)ns for use
Deepwell Plates and Microplates
Instructions for use
Copyright ©2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.
Eppendorf
®
, the Eppendorf logo, and Eppendorf Plate® are registered trademarks of
Eppendorf AG, Hamburg, Germany.
Registered trademarks are not marked in all cases with ® in this manual.
0012 577.710-01/042013
Product description
Deepwell Plates and Microplates
English (EN)
1 Product description
Eppendorf Deepwell Plates und Microplates are uncoated polypropylene single-use items
for analyzing, preparing, mixing, centrifuging, transporting and storing of solid and liquid
probes. Eppendorf Microplates can be used in all conventional plate readers. Only trained
technical staff may use these consumables.
Eppendorf Deepwell Plates und Microplates are characterized by a high chemical and
temperature resistance and can be sealed using suitable sealing options. The plates meet
the Microplate Standards ANSI/SLAS 1-2004 through ANSI/SLAS 4-2004.
This product can be used in development, research, routine and training laboratories in
industry, in chemistry, and in training and instruction. The applications extend over the
areas of life science, biotechnology, chemistry and clinical research, but are not limited to
these areas.
2 Safety
2.1 Intended use
Eppendorf Deepwell Plates and Microplates are single-use items made from polypropylene for preparing, mixing, centrifuging, transporting and storing solid and liquid samples
and reagents. The field of application is training, routine and research laboratories in the
areas of life sciences, industry, medical and pharmaceutical research and chemistry.
Eppendorf Microplates can be used in all conventional plate readers.
Operation requires skilled personnel who have been trained for the above mentioned
areas.
2.2 Warnings for intended use
Please read this manual thoroughly before using the Eppendorf Deepwell Plates and
Microplates for the first time.
Please view this manual as part of the product and keep it somewhere easily
accessible.
If this manual is lost, please request another one. The current version is available on
our webpage www.eppendorf.com, also in other languages.
3
WARNING! Damage to health from toxic, radioactive or aggressive chemicals
as well as infectious liquids and pathogenic germs.
Observe the national regulations for handling these substances, the biological
security level of your laboratory, the material safety data sheets and the
manufacturer's application notes.
Wear personal protective equipment.
Safety
4
Deepwell Plates and Microplates
English (EN)
WARNING! Contamination risk from splashing liquids or escaping
substances.
Observe the maximum filling volume.
Take appropriate safety measures to prevent the escape of substances in the
plates during storage and transportation.
Always remove the covers carefully.
Use only undamaged and visibly perfect plates from intact packages.
Do not use liquid nitrogen in combination with the plates. They could be
damaged or the covers could burst open during thawing.
WARNING! Contamination risk from damaged plates during centrifugation.
The plates withstand heavy loads during centrifugation. However, they can be
destroyed and release the substances they contain if used improperly.
Always observe the maximum permitted centrifugation forces.
Read the operating manual of the centrifuge used.
Centrifuge stacked plates at lower speed only.
Note that organic solvents may reduce the mechanical load capability of the
plates. If in doubt please contact the Eppendorf Application Support
(support@eppendorf.com).
CAUTION! Contamination risk when disposing of used consumables.
Dispose of used consumables in accordance with the substances with which
they have come into contact.
Observe the rules applicable to your lab.
NOTICE! Material change of consumables due to extreme temperatures.
Extreme temperatures (e.g., during refrigeration or autoclaving) affect
consumables material. The mechanical strength, dimensions and shape of the
consumable will change.
Use consumables that are suitable for the selected temperature range or
selected procedure.
Technical data
Deepwell Plates and Microplates
English (EN)
3 Technical data
Material Polypropylene, colored and colorless
Chemical resistance The plates, including border, show a high resistance to UV
light and chemicals. Compare Application Note 56: The best
material for original Eppendorf Tubes® and Plates on
www.eppendorf.com. In case of doubt, contact Eppendorf
Application Support.
Dimensions In accordance with Microplate Standards ANSI/SLAS 1-2004
through ANSI/SLAS 4-2004
1
.
Autoclaving Autoclavable (121 °C, 20 min.). The dimensional stability of
the single-use items can be affected.
Operational temperature -86 °C to +100 °C
Storage before use Store dry at room temperature. Protect from direct sunlight
and UV light.
Current certificates can be found at www.eppendorf.com.
1
SLAS: Society for Laboratory Automation and Screening
3.1 Maximum values for centrifuging
NOTICE! Consumables will get hot.
In non-refrigerated centrifuges, the temperature in the rotor chamber, rotor and
sample can increase to above 40 °C, based on the run time, g-force (rcf)/speed
and ambient temperature.
Please note that the resistance to centrifugation of the tubes and plates will
reduce.
Please note the temperature resistance of the samples.
5
Centrifugation at 4 °C – 40 °C possible
The plates can be centrifuged with the maximum g-forces (rcf) listed in the table below
under the following conditions:
• in a 45° fixed-angle rotor
• sample temperature of 40°C with aqueous saline solution (1.0 g/mL density)
• for 90 min
Sterile PCR clean
Protein LoBind
DNA LoBind
Eppendorf Deepwell Plates 5 000 × g 6 000 × g
Eppendorf Microplates 6 000 × g 6 000 × g
The mechanical strength of the plates is reduced by the use of organic solvents. If in
doubt, please contact the Eppendorf Application Support
The centrifugation stability of any plate type is generally dependent on the plate itself,
centrifuge, rotor, rotor bucket, adapter, maximum rotation/maximum gravitation field,
Technical data
6
Deepwell Plates and Microplates
English (EN)
operating temperature, centrifugation time, density of centrifuged liquid, organic solvent,
accumulated total weight and correct operation.
3.2 Eppendorf Deepwell Plate
Eppendorf Deepwell Plate 96/2000 µL 96/1000 µL 96/500 µL 384/200 µL
Theoretical total
[μL] 2400 1200 700 240
volume
Working volume [μL] 50 - 2000 30 - 1000 30 - 550 20 - 225
Position Well
[mm] a/b 11.2/14.4 9.0/12.1
center A1
Distance of well
Well/cavity
center
[mm] C 9.0 4.5
Depth [mm] 41 41 24 22
Length [mm] l 127.8
Width [mm] W 85.5
Height [mm] h 44.1 44.1 27.1 25.1
Plate
Base height [mm] bh 6.1
Stacking height
*
SH
*
The height of a plate stack is made up of a base plate of height h and other stacked
[mm] 42.7 42.7 25.7 23.7
plates each of stacking height SH.
Technical data
Deepwell Plates and Microplates
English (EN)
3.3 Eppendorf Microplate
Eppendorf Microplate 96/F-PP 96/U-PP 96/V-PP 384/F-PP 384/V-PP
Bottom shape flat round V-shape flat V-shape
Theoretical total
[μL] 400 360 350 150 140
volume
Working volume [μL] 50 - 350 20 - 320 20 - 300 10 - 120 5 - 120
Position Well
[mm] a/b 11.2/14.4 9.0/12.1
center A1
Well/cavity
Distance of well
[mm] C 9.0 4.5
center
Depth [mm] 11.2 11.1 11.2 11.6 11.6
Length [mm] l 127.8
Width [mm] W 85.5
Height [mm] h 14.4
Plate
Base height [mm] bh 6.1
Stacking height
*
SH
*
The height of a plate stack is made up of a base plate of height h and other stacked
[mm] 13.0
plates each of stacking height SH.
7
Ordering Information
8
Deepwell Plates and Microplates
English (EN)
3.4 Eppendorf purity grades
Sterile PCR clean
Batch testing (certified) for the following purity criteria:
Free of human DNA ■
DNase-free ■
RNase-free ■
Free of PCR inhibitors ■
Pyrogen-free (endotoxin-free) ■
Sterile (Ph.Eur./UPS) ■
4 Ordering Information
The Eppendorf Deepwell Plates and Microplates are also available in the following
variants:
• Deepwell Plates: other border colors (yellow, green or blue).
• Large packs.
• Upon request with barcodes (
You can find ordering information on suitable sealing options on
consumables.
Further product information is available in our catalogue, on our webpage
www.eppendorf.com/consumables, and from Eppendorf Application Support.
www.eppendorf.com/barcode).
www.eppendorf.com/
4.1 Eppendorf Deepwell Plates
Order no.
(International)
0030 501.306
0030 502.302
0030 504.305
0030 501.209
0030 502.205
0030 503.201
0030 504.208
Order no.
(North America)
951033405
951033502
0030504305
951032603
951032701
951032808
951032905
Description
Eppendorf Deepwell Plate 96/2000 µL
20 plates, wells clear, white border color
PCR Clean
Sterile
Protein LoBind
Eppendorf Deepwell Plate 96/1000 µL
20 plates, wells clear, white border color
PCR Clean
Sterile
DNA LoBind (also for RNA and other nucleic acids)
Protein LoBind
Ordering Information
Deepwell Plates and Microplates
English (EN)
9
Order no.
(International)
0030 501.101
0030 502.108
0030 503.104
0030 504.100
0030 521.102
0030 522.109
0030 523.105
0030 524.101
Order no.
(North America)
951031801
951031909
951032000
951032107
951031003
951031101
951031208
951031305
4.2 Eppendorf Microplates
Order no.
(International)
0030 601.106
0030 602.102
0030 601.475 0030601475
0030 601.700 0030601700
0030 601.203
0030 602.200
0030 601.807 951040102
0030 601.572 951040145
0030 601.300
0030 602.307
Order no.
(North America)
951040005
951040021
951040048
951040081
951040188
951040227
Description
Eppendorf Deepwell Plate 96/500 µL
40 plates, wells clear, white border color
PCR Clean
Sterile
DNA LoBind (also for RNA and other nucleic acids)
Protein LoBind
Eppendorf Deepwell Plate 384/200 µL
40 plates, wells clear, white border color
PCR Clean
Sterile
DNA LoBind (also for RNA and other nucleic acids)
Protein LoBind
Description
Microplate 96/F-PP
80 plates, wells clear, white border color
PCR clean
Sterile
Microplate 96/F-PP
80 plates, wells white, gray border color
PCR clean
Microplate 96/F-PP
80 plates, wells black, white border color
PCR clean
Microplate 96/U-PP
80 plates, wells clear, white border color
PCR clean
Sterile
Microplate 96/U-PP
80 plates, wells black, white border color
PCR clean
Microplate 96/U-PP
80 plates, wells white, gray border color
PCR clean
Microplate 96/V-PP
80 plates, wells clear, white border color
PCR clean
Sterile
Ordering Information
10
Deepwell Plates and Microplates
English (EN)
Order no.
(International)
Order no.
(North America)
0030 601.904 951040260
0030 601.670 951040308
0030 621.107
0030 622.103
0030 621.301
0030 622.308
0030 623.304
0030 624.300
951040341
951040383
951040421
951040464
951040546
951040589
0030 621.905 951040481
0030 621.670 951040503
Description
Microplate 96/V-PP
80 plates, wells black, white border color
PCR clean
Microplate 96/V-PP
80 plates, wells white, gray border color
PCR clean
Microplate 384/F-PP
80 plates, wells clear, white border color
PCR clean
Sterile
Microplate 384/V-PP
80 plates, wells clear, white border color
PCR clean
Sterile
DNA LoBind
Protein LoBind
Microplate 384/V-PP
80 plates, wells black, white border color
PCR clean
Microplate 384/V-PP
80 plates, wells white, gray border color
PCR clean
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Eppendorf AG · 22331 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com
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