Eppendorf Concentrator plus User Manual

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Concentrator plus/Vacufuge® plus

Operating manual

5305 900.038

2011 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of

the copyright owner.

Trademarks

eppendorf, CombiSlide, and Vacufuge are registered trademarks of Eppendorf AG, Hamburg, Germany. Falcon and Microtainer are a registered trademarks of Becton Dickinson, Franklin Lakes, NJ, USA.

Trademarks are not marked in all cases with ™ or

in this manual.

Operating manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211

5305 900.038-02/122011



Abb. 1: Fig. A: Front and rear view of the complete system with gel dryer conn ection

12 3

10111213

Fig. A: Front and rear view of the complete system with gel dryer connection

Abb. 2: Fig. B: Front and rear view of the basic device

15910711 8

Fig. B: Front and rear view of the basic device

1 Pump outlet (complete system) 2 Lid made from PMMA

3 Sealing ring 4 Grip recess

5 Emergency release 6 Pump housing (complete system)

7 Name plate 8 Mains connection

9 Fuse holder 10 Mains power switch

11 Operating controls and device display 12 Emission condenser (complete system)

13 Gel dryer connection (complete system) 14 Pump connection (basic device)

15 Mains connection for external vacuum pump

(basic device)

Abb. 3: Fig. C: Operating controls and device display for Concentrator plus and Vacufuge plus

2 3 4

8 71011

Fig. C: Operating controls and device display for Concentrator plus and Vacufuge plus

Abb. 4: Fig. D: Connection optio ns for the complete s ystem

emission

condenser

filter/ hood

cold trap

chemical

trap

Fig. D: Connection options for the complete system

Abb. 5: Fig. E: Connection options for the basi c device

Fig. E: Connection options for the basic device

cold trap

solenoid

valve

cold

trap

pump

cold

trap

solenoid

valve

emission

condenser

cold trap

external vacuum

system

filter/ hood

Concentrator plus/Vacufuge® plus — Operating manual

Operating manual — Concentrator plus / Vacufuge® plusseeon p.Fig.Tab.p.

Operating manual

Table of contents

1 Table of contents

1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2 Warning signs and hazard icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.3 Symbols used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.4 Abbreviations used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.3.1 Complete system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.3.2 Basic device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.2 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.4 Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.4.1 Special notes on the individual rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.3 Application limits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.3.1 Declaration concerning the ATEX directive (94/9/EC). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.5.2 Incorrect handling of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.5.4 Extreme strain on the sample tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3.5.5 Vacuum. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.6 Safety instructions on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.2 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.2.1 Open the packaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.2.2 Lift the device out of the box. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.2.3 Complete system: removing the transport protection screw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.2.4 Place the device upright . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.2.5 Check the delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.3 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4.3.1 General Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4.3.2 Complete system: connecting the emission condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.3.3 Complete system: connecting the gel dryer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.3.4 Basic device: connecting the vacuum pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.1 Overview of operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.2 Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.3 Preparation for concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.3.1 Switching on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.3.2 Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.3.3 Warm-up phase. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.3.4 Loading a fixed-angle rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

5.3.5 Loading a swing-bucket rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

5.3.6 Closing the lid of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

. . . . . . 29

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

5.4 Concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.4.1 Concentration with time setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.4.2 Concentration with continuous run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.4.3 During or after concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.4.4 Completing an application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.4.5 Removing the rotor and switching off the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.4.6 Emptying the emission condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.5 Notes on using rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.5.1 RotorA-2-VC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.6 Special function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.6.1 Operation as a desiccator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.6.2 Operation with gel dryer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.6.3 Operation as a centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

6.1 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

6.1.1 Device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

6.1.2 Pump. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

6.2 Prepare cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

6.3 Cleaning / Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

6.3.1 Cleaning / Disinfecting the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

6.3.2 Cleaning / Disinfecting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

6.4 Glass breakage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

6.5 Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

6.6 Decontamination before shipping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

7.3 Opening the device in case of a power failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

8.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

8.2 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

8.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

9.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

9.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

9.3 Weight / dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

9.4 Diaphragm vacuum pump for the complete system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

9.5 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

10 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

10.1 Complete system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

10.2 Basic device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

10.3 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

10.4 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

10.4.1 Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

10.4.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

10.4.3 Other accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

Concentrator plus/Vacufuge® plus — Operating manual

1 User instructions

1.1 Using this manual



Read this operating manual completely before using the device for the first time.

 Consider this operating manual as part of the product and keep it in a place where it can be

easily accessed.

 Include this operating manual with the device when passing the device on to third parties.  In case of loss of the operating manual, please request a replacement. The latest version can

be found on our website at www.eppendorf.com

1.2 Warning signs and hazard icons

1.2.1 Hazard symbols

Biohazard Explosion

Electric shock Hot surface

Crushing Toxic substances

Hazard point Material damage

Operating manual

1.2.2 Degrees of danger

The degree of danger is a part of a safety note and distinguishes the possible results of non-observance from each other.

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Symbols used

Depiction Meaning

 You are requested to perform an action.

Perform these actions in the sequence described.

• List.

References useful information.

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

1.4 Abbreviations used

ETFE Ethylene/Tetrafluorethylene copolymer

FFKM Perfluoroelastomer

MTP Micro test plate

NN Mean sea level (MSL)

PCR Polymerase Chain Reaction

PMMA Polymethyl methacrylate

PTFE Polytetrafluorethylene

rcf Relative centrifugal force (g value, RZB)

-1

rpm Revolutions per minute (min

RCF relative centrifugal force (g value)

UV Ultraviolet radiation

)

Concentrator plus/Vacufuge® plus — Operating manual

2 Product description

2 Produc t description

2.1 Main illustration

The fold-out page depicts front and rear views of the complete system (see Fig. A) and the basic device (see Fig. B).

2.2 Features

Concentrator plus and Vacufuge plus are designed for the evaporation of liquid or wet samples in micro test tubes, round-bottom tubes, Falcon tubes, flat-bottom tubes and different plates.

You can insert the following tubes and plates:

A complete list can be found in the following chapter (see Rotors on p. 11) The device is available as a complete system with integrated diaphragm vacuum pump or as a

basic device without vacuum pump. The basic device can be connected to an external vacuum system.

Key, practical functions of Concentrator plus or Vacufuge plus have been optimized:

Due to the space-saving design, the device can be situated directly on the workstation. With its clearly laid-out control panel with only a few, clearly marked elements and a display that is easy to read, the device is a pleasant and reliable companion in your daily work.

1 Pump outlet (complete system)

Outlet of the integrated diaphragm vacuum pump, e.g., for tubing connection to the emission condenser.

3 Sealing ring 4 Grip recess

5 Emergency release 6 Pump housing (complete system)

7 Name plate 8 Mains connection

9 Fuse holder 10 Mains power switch

11 Operating controls and device display 12 Emission condenser (complete

13 Pump connection (basic device)

For tubing connection to external vacuum pump.

2 Lid made from PMMA

system)

14 Mains connection for external vacuum

pump (basic device)

• 1.5 and 2.0 mL micro test tubes in a rotor with 70 places.

• 0.5 mL micro test tubes in a rotor with 72 places.

• 15 mL Falcon tubes in a rotor with 8 places.

• 50 mL Falcon tubes in a rotor with 6 places.

• MTP and PCR plates in a swing-bucket rotor.

• 3 temperature levels can be set (30, 45, 60 °C). Alternatively, evacuation is carried out

without thermoregulation.

• The evaporation of liquids can be carried out optimized in 3 functions. In addition to pure

evacuation, aqueous and/or alcoholic solutions can be concentrated extra fast with 2 special functions.

• You can also operate the device as a pure desiccator.

• You can connect a solvent trap to the device behind the pump.

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

2.3 Delivery package

The delivery package includes one of the following device/rotor combinations and the accessories listed below.

2.3.1 Complete system

Operating manual

Quantity Order No.

(International)

1 or or

or or or

1 or

1- - Power cable 1 5305 900.038 - Operating Manual Concentrator plus/Vacufuge plus

1 5301 330.008 022830309

1 5301 337.002 022830295

1 5301 110.032 5301110032

5305 000.304 5305 000.312 5305 000.347

5305 000.509 5305 000.517 5305 000.541

5305 000.703 5305 000.711 5305 000.746

5301 850.249 5417 341.007

Order No. (North America)

5305000312

5305000517

5305000711

022654403 022375831

Description

Concentrator plus/Vacufuge plus complete system

with rotor F-45-48-11 230 V/50 - 60 Hz, with integrated diaphragm vacuum pump 120 V/50 - 60 Hz, with integrated diaphragm vacuum pump 100 V/50 - 60 Hz, with integrated diaphragm vacuum pump

Concentrator plus/Vacufuge plus complete system

without rotor 230 V/50 - 60 Hz, with integrated diaphragm vacuum pump 120 V/50 - 60 Hz, with integrated diaphragm vacuum pump 100 V/50 - 60 Hz, with integrated diaphragm vacuum pump

Concentrator plus/Vacufuge plus complete system

with connection, e.g., for a gel dryer, without rotor 230 V/50 - 60 Hz, with integrated diaphragm vacuum pump 120 V/50 - 60 Hz, with integrated diaphragm vacuum pump 100 V/50 - 60 Hz, with integrated diaphragm vacuum pump

Fuse

2 x 4.0 A T (230 V) 2 x 6.3 A T UL (120 V / 100 V)

Emission condenser

without hose

Hose for emission condenser

length: 0.7 m

Tube connection

Plastic

2.3.2 Basic device

Quantity Order No.

(International)

1 or or

1 or

1- - Power cable 1 5305 900.038 - Operating Manual Concentrator plus/Vacufuge plus

5305 000.100 5305 000.118 5305 000.142

5301 850.249 5417 341.007

Order No. (North America)

5305000118

022654403 022375831

Description

Concentrator plus/Vacufuge plus basic device

with rotor F-45-48-11 230 V/50 - 60 Hz 120 V/50 - 60 Hz 100 V/50 - 60 Hz

Fuse

2 x 4.0 A T (230 V) 2 x 6.3 A T UL (120 V / 100 V)

Concentrator plus/Vacufuge® plus — Operating manual

2.4 Rotors

You can operate the device with the following rotors. Before use of sample tubes, please note the manufacturer's specifications with regard to centrifugation resistance (max. rcf).

Rotor Tube Max. capacity Tube dimensions

(mm) Ø × L  and/or L × W × H

Max. g-force (rcf)/ speed (rpm)

Max. payload per rotor bore

Operating manual

(1)

F-45-72-8 72 micro test tubes of

0.5 mL each.

F-45-70-11 70 micro test tubes of

1.5/2.0 mL each.

F-45-48-11 48 micro test tubes

of 1.5/2.0 mL each.

F-45-24-12 24 round-bottom tubes

of 6.0/8.0 mL each.

F-50-8-16 8 round-bottom tubes

of 15.0/20.0 mL each.

F-50-8-18 8 round-bottom tubes

of 15.0/20.0 mL each.

8 × 31 inner ring: 

202 x g, outer ring: 

224 x g/ 1,400 rpm

11 × 41/ 11 × 47

12 × 67-100 234 x g/

16 × 105-120 230 x g/

18 × 105-128 230 x g/

inner ring:  153 x g,

middle ring: 202 x g,

outer ring:  248 x g/

1,400 rpm

inner ring:  217 x g,

outer ring:  239 x g/

1,400 rpm

10 g

15 g

35 g

70 g

F-35-6-30 6 Falcon tubes

of 15 mL each.

F-45-8-17 8 Falcon tubes

of 15 mL each.

F-35-6-30 6 Falcon tubes

of 50 mL each.

17 × 116-123 232 x g/

1,400 rpm

17 × 118-123 239 x g/

1,400 rpm

29.5 × 116-123 232 x g/ 1,400 rpm

25 g

70 g

75 g

Concentrator plus/Vacufuge® plus — Operating manual

Rotor Tube Max. capacity Tube dimensions

(mm) Ø × L  and/or L × W × H

Max. g-force (rcf)/ speed (rpm)

Max. payload per rotor bore

(1)

Operating manual

F-40-36-12 36 flat-bottom tubes of

1.5 mL each.

F-45-36-15 36 flat-bottom tubes of

3.0/5.0 mL each.

F-45-16-20 16 flat-bottom tubes

of 6.5/10.0 mL each.

F-40-18-19 18 flat-bottom tubes of

10.0 mL each.

F-45-12-31 12 flat-bottom tubes of

20.0 mL each.

F-35-8-24 8 flat-bottom tubes of

25.0 mL each.

A-2-VC Two buckets to hold

• 0.2 mL PCR tubes

• PCR strips of 8

and of 5

• PCR plates

• Microplates

• Cell culture plates

• Deepwell plates

(max. height = 27 mm)

• Slides (with

CombiSlide adapter)

12 × 32 215 x g/

1,400 rpm

15 × 45-48 217 x g/

1,400 rpm

20 × 42-55 217 x g/

1,400 rpm

19 × 66 228 x g/

1,400 rpm

31 × 55 226 x g/

1,400 rpm

24 × 86-90 232 x g/

1,400 rpm

128 × 86 × 27 131 x g/

1,400 rpm

26 × 75

15 g

20 g

45 g

35 g

55 g

90 g

115 g per bucket

(1) Maximum payload per rotor bore for adapter + tube + content.

Concentrator plus/Vacufuge® plus — Operating manual

2.4.1 Special notes on the individual rotors

Rotor F-45-72-8 and rotor F-45-48-11

• You can place two of these rotors on top of each other in any arrangement by using a spacer

(order no. int.: 5301 316.005 / North America: 022822101) and centrifuge them at the same time.

• For this combination, lower upper limits apply to the maximum payload: bottom rotor: 2/3 of

the max. payload, top rotor 1/3 of the max. payload.

• Rotor F-45-72-8 and F-45-48-11: Included with the accessories are rotor feet which can be

screwed into the corresponding bores on the rotor. Their purpose is to prevent the samples from being pushed out of the bores when the rotor is put down on the work bench. The rotors can be centrifuged with the rotor feet. The screws must be checked monthly and re-tightened, if required.

Operating manual

Rotor F-45-24-12

• Tube length  75 mm: max. capacity 24 tubes.

• Tube length > 75 mm: max. capacity 12 tubes.

Rotor A-2-VC

• Tubes, PCR strips and plates without frame can only be used with a suitable work plate

(see Adapters on p. 44).

• Max. loading height: 27 mm.

Rotor F-35-6-30

• The recommended maximum filling volume for 50 mL Falcon tubes is 44 mL. If the tubes are

filled up to the top, the rotational movement may cause the samples to overflow.

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

3 Safety

3Safety

3.1 Intended use

Concentrator plus and Vacufuge plus are intended for sample preparation. The device may only be operated by trained specialist staff.

Concentrator plus and Vacufuge plus are intended for interior use only and mainly intended for the concentration of aqueous solutions of nucleic acids and proteins in approved sample tubes.

Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may

CAUTION!

3.2 User profile

impair the safety, function and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts or from the improper use of such equipment.

 Only use accessories and original spare parts recommended by Eppendorf.

This device may only be operated by correspondingly trained specialist staff. This staff must have carefully read the operating manual and be familiar with the function of the device.

3.3 Application limits

Continuous delivery can affect safe operation of the device.

When delivering liquids continuously, membranes and valves of the vacuum pump can be

CAUTION!

3.3.1 Declaration concerning the ATEX directive (94/9/EC)

damaged.

 Only use the device for applications of limited duration.

Danger of explosion!

 Do not operate the device in areas where work is completed with explosive substances.

DANGER!

 Do not use this device to process any explosive or highly reactive substances.  Do not use this device to process any substances which could create an explosive

atmosphere.

Due to their current design and the environmental conditions on the inside of the device, the Concentrator plus / Vacufuge plus are not suitable for use in a potentially explosive atmosphere.

The device must therefore only be used in a safe environment, such as in the open environment of a ventilated laboratory or an extractor hood. The use of substances which may contribute to a potentially explosive atmosphere is not permitted. The final decision with regard to the risks connected with the use of such substances is the responsibility of the user.

3.3.2 Maximum service life for accessories

The maximum service life for plastic adapters is 1 year from first commissioning. Do not use the adapter beyond this period!

For the rotors (see Rotors on p. 11) described here there is no limit for their service life, as long as the following conditions are met: proper use, recommended maintenance and undamaged condition.

Concentrator plus/Vacufuge® plus — Operating manual

3.4 Information on product liability

In the following cases, the protection provided by the device may be impaired. The liability for the function of the device passes to the operator if:

• The device is not used in accordance with the operating manual.

• The device is used outside of the range of application described in the preceding chapters.

• The device is used with accessories or consumables (e.g., tubes and plates) which are not

recommended by Eppendorf.

• The device is maintained or repaired by persons not authorized by Eppendorf.

• The owner has made unauthorized modifications to the device.

3.5 Warnings for intended use

Before using the device, read the operating manual and observe the following general safety instructions.

3.5.1 Personal injury or damage to the equipment

Electric shock from damage to device or mains cable.

 Only switch on the device if the device and the mains cable are undamaged.

WARNING!

 Only use devices that have been properly installed or repaired.  In case of danger, disconnect the device from the mains.

Operating manual

WARNING!

Lethal voltages inside the device.

 Ensure that the housing is always closed and undamaged so that no parts inside the device

can be contacted by accident.

 Do not remove the housing of the device.  Do not allow any liquids to penetrate the inside of the housing.  Do not allow the device to be opened by anyone except service personnel who have been

specifically authorized by Eppendorf.

Risk from incorrect supply voltage.

 Only connect the device to power sources which correspond with the electrical requirements

on the name plate.

 Only use sockets with a protective earth (PE) conductor and a suitable mains cable.

Damage to health due to handling infectious liquids and pathogenic germs.

 Observe the national regulations for handling these substances, the biological security level

of your laboratory, the safety data sheets and the manufacturer's application notes.

 Wear personal protective equipment (PPE).  Follow the instructions regarding hygiene, cleaning and decontamination.  For complete instructions regarding the handling of germs or biological material of risk group

II or higher, please refer to the "Laboratory Biosafety Manual" (source: World Health Organization, current edition of the Laboratory Biosafety Manual).

WARNING!

Burns due to hot sample tubes and device parts.

If the heater is at full power, the wall of the rotor chamber, the rotor and the sample tubes will be heated up to over 60°C.

 Do not touch the wall of the rotor chamber.  Use suitable protective clothing or auxiliary aids to remove the sample tubes.

Concentrator plus/Vacufuge® plus — Operating manual

Damage to device by spilling liquids in the rotor or rotor chamber.

1. Switch the device off.

NOTICE!

2. Disconnect the device from the power supply.

3. Clean the device and the accessories carefully in accordance with the cleaning and disinfection instructions in the operating manual.

4. If a different cleaning and disinfecting method is to be used, contact Eppendorf AG to ensure that the intended method will not damage the device.

Operating manual

Device damage due to organic solvents.

The use of organic solvents may cause some parts to become damaged, e.g., discolored.

NOTICE!

3.5.2 Incorrect handling of the device

 If the device becomes contaminated with organic solvents, clean it immediately with a mild

cleaning agent.

Damage to electronic components due to condensation.

After the device has been moved from a cool to a warmer environment, condensation can form inside the device.

 Wait at least three hours before connecting the device to the power supply.

Device lid can crush. Keep hands clear.

 Do not reach between the device and lid when opening or closing the device lid.

WARNING!

Damage from knocking against or moving the device during operation.

A rotor banging against the rotor chamber wall can cause considerable damage to the device and

NOTICE!

rotor.

 Do not move or knock against the device during operation.

3.5.3 Incorrect handling of the rotors

Risk of injury from improperly attached rotors.

 Only operate the device if the rotor has been mounted completely.

WARNING!

 If there are any unusual noises when the device is started up, immediately press the start/

stop key to stop concentrating.

Risk of injury from unsymmetric loading of rotors.

 Load rotors symmetrically with identical tubes and/or buckets and plates.

CAUTION!

 Only load adapters with suitable tubes and/or plates.  Always use tubes and/or plates of the same type (weight, material/density and volume).  Ensure that tubes that are located opposite each other contain liquids with the same rate of

evaporation. Otherwise an imbalance can occur and the concentration may automatically stop.

 Check for symmetric loading by balancing the adapters and tubes and/or plates used with

scales.

The device automatically detects imbalances during operation and stops the run immediately with an error message and a signal tone. Check the load, balance the tubes and restart the run.

Concentrator plus/Vacufuge® plus — Operating manual

Risk of injury from overloaded rotor.

The device is designed for the concentration of substances with a max. density of 1.2 g/mL at

CAUTION!

NOTICE!

maximum speed and filling volume and/or load.

 Note the maximum payload for each rotor (adapter, tube and content) per rotor bore and/or

per bucket and make sure it is not exceeded.

Risk of injury from chemically or mechanically damaged accessories.

Even small scratches and cracks can lead to serious internal material damage.

 Protect all parts from mechanical damage.  Check accessories regularly.  Do not use rotors or buckets with signs of corrosion or mechanical damage (e.g.

deformations).

 Do not use accessories whose maximum service life has been exceeded.  When inserting the buckets and rotors, ensure that they do not become scratched.

Damage to rotors from aggressive chemicals.

Rotors are high-quality components which withstand extreme stresses. This stability can be impaired by aggressive chemicals.

 Avoid the use of aggressive chemicals, including strong and weak alkalis, strong acids,

solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons, concentrated saline solutions and phenol.

 If the rotor is contaminated with aggressive chemicals, clean it immediately using a neutral

cleaning agent and then rinse it thoroughly with water. This applies to the base plates of the rotors in particular.

Operating manual

3.5.4 Extreme strain on the sample tubes

Risk of injury from overloaded tubes.

 Note the loading limits specified by the tube manufacturer.

CAUTION!

NOTICE!

 Only use tubes which are approved by the manufacturer for the required rcf.

Risk from damaged tubes.

Damaged tubes must not be used, as this could cause further damage to the device and the accessories and loss of the samples.

 Before use, carry out a visual check of all tubes for any damage.

Hazard to plastic tubes from organic solvents.

When using organic solvents (e.g. phenol, chloroform) the strength of plastic tubes is reduced, i.e., the tubes could get damaged.

 Follow the manufacturer's information about the chemical resistance of tubes.

Sample tubes are subject to strong heat.

 Please note the temperature resistance of the sample tubes.

NOTICE!

CAUTION

Outlet for the air ﬂow on top. Outlet only.

Do not cover.

▲

5305 001.521-00

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

3.5.5 Vacuum

Risk of injury from direct contact with the vacuum.

 Do not expose any body parts to the vacuum of the device.

WARNING!

Risk of injury from overpressure.

Overpressure in the exhaust gas lines (e.g., from closed taps or blocked pipes) can cause the

WARNING!

pipes to burst.

 Only use pipes with a sufficiently large cross section.  Always keep the exhaust gas line free.  Do not place any objects on the exhaust gas lines.  Do not bend the exhaust gas lines.  Do not fit any hose clamps or valves to the exhaust gas lines.  Note the maximum pressures and differential pressures permitted (see p. 41).

Risk to health from escaping substances.

No vapors from toxic liquids and pathogenic germs must escape.

WARNING!

 Ensure that there is sufficient condensation and/or separation of the vapors via suitable cold

traps or chemical traps.

 Please note the personal protective equipment (gloves, clothing, goggles, etc.), extraction

and safety class required for the laboratory.

3.6 Safety instructions on the device

Depiction Meaning Location

General hazard area

 Follow the operating manual.

Complete system: side of the device next to the mains connection.

Basic device: rear next to the mains connection.

WARNING

Danger of explosion

Top of the device

 Do not use this device to process any

explosive, radioactive or highly reactive

5305 100.902-00

substances.

 Do not use this device to process any

substances which could create an explosive atmosphere.

CAUTION

Risk of scalding when the device lid is open

Top of the device

When the heater is switched on, the surface temperature of the rotor chamber can be > 60 °C.

5305 100.910-00

 Do not touch the wall of the rotor chamber.

Risk of injury from overpressure

Top of the emission condenser

The connection on the top of the emission condenser is intended as an outlet only.

 Make sure that the emission condenser is

connected correctly.

 Never shut this connection off.

Concentrator plus/Vacufuge® plus — Operating manual

4 Installation

4 Install ation

4.1 Selecting the location

If a fault occurs, objects in the immediate vicinity of the device could get damaged.

 In accordance with the recommendations of EN 61010-2-020, leave a safety distance of 30

NOTICE!

cm clear around the device during operation.

Damage from overheating.

 Do not place the device close to sources of heat (e.g., radiator, drying cabinet).

NOTICE!

 Do not expose the device to direct sunlight.  Ensure free circulation of air by maintaining a distance of at least 30 cm on all sides of the

device from adjacent devices or the wall and keep the underside of the device clear.

 Make sure that the air slots in the device are always free of obstruction.

Select the location for the device according to the following criteria:

• Suitable power connection as per the name plate (230 V/120 V/100 V).

• Stable, horizontal and resonance-free lab bench. Weight of the device: 31/17 kg (complete

system/basic device).

• A well ventilated environment which is protected from direct sunlight to prevent the device

from heating up more.

• Ambient temperature during operation: 15 to 35°C.

• At altitudes 1000 m above MSL (risk of insufficient cooling air supply), measures in

accordance with DIN EN 60034-1; VDE 0530-1 are required.

Operating manual

4.2 Preparing installation

The weight of the device is 31.5 kg (complete system incl. gel dryer) or 16.5 kg (basic device).

Hint!

• Transport and installation of the device always requires 2 persons.

• Only transport the device in the original packaging.

• Use a transport aid for longer distances (e.g., hand cart).

• Keep the packaging and transport securing devices for later transport or storage. See also

the instructions relating to transport (see p. 39).

 Complete the following steps in the order described:

4.2.1 Open the packaging

1. Cut the adhesive tape.

2. Open up all four tabs of the box.

3. Remove the accessories and rotors.

4. Remove the cover cardboard.

5. Cut the strap retainers.

4.2.2 Lift the device out of the box

1. Lift the device and transport protection pad out of the box.

2. Place the device and transport protection pad on a stable, level and non-resonant lab bench.

Concentrator plus/Vacufuge® plus — Operating manual

4.2.3 Complete system: removing the transport protection screw

1. Carefully place the device and transport protection pad on its side.

Abb. 1: Transport protection screws on the underside of the Con centrator plus/Vacufug e plus complete system

Operating manual

Fig. 1: Transport protection screws on the underside of the Concentrator plus/Vacufuge plus

1 Rear transport protection screw 2 Front transport protection screw

2. Lightly move the rear transport protection pad on the underside of the device towards the back until the rear transport protection screw (1) is visible.

3. Unscrew and remove the two transport protection screws.

4. Push the rear transport protection pad back to its original position.

5. Place the device and transport protection pad upright again.

4.2.4 Place the device upright

Operating an incorrectly installed device poses a risk of injury.

The device is not ready for operation after it has been placed upright. Additional components are

WARNING!

4.2.5 Check the delivery

missing.

 Read the following chapter before operating the device (see Installing the instrument on

p. 21).

1. Remove the front and rear transport protection pads.

2. Remove the plastic coverings from the device and lid.

3. Place the device upright in a well-ventilated area protected from direct sunlight to prevent it from continuing to heat up.

1. Check that delivery is complete (see Delivery package on p. 10).

2. Check all parts for any transport damage. Please contact your distributor if there is any damage.

complete system

Proceed in reverse order when packing the Concentrator plus/Vacufuge plus.

Hint!

Concentrator plus/Vacufuge® plus — Operating manual

4.3 Installing the instrument

4.3.1 General Installation

Risk from incorrect supply voltage.

 Only connect the device to power sources which correspond with the electrical requirements

WARNING!

Hint!

on the name plate.

 Only use sockets with a protective earth (PE) conductor and a suitable mains cable.

Risk to health from escaping substances.

No vapors from toxic liquids and pathogenic germs must escape.

 Ensure that there is sufficient condensation and/or separation of the vapors via suitable cold

traps or chemical traps.

 Please note the personal protective equipment (gloves, clothing, goggles, etc.), extraction

and safety class required for the laboratory.

Select a layout for the collection and/or discharge of liquids and gases which corresponds with the current legal requirements and regulations for your application.

Operating manual

On the fold-out page of this operating manual you can find schematic representations for possible combinations of the complete system and/or basic devices as well as other components (refer to. Fig. D and Fig. E).

Perform the following steps in the sequence described.

1. Place the device on a suitable lab bench.

2. Allow the device to warm up to ambient temperature for at least 3 hours to prevent damage to electronic components from condensation.

3. Connect the components according to your chosen configuration. Please note the relevant instructions in the following chapters.

• Complete system: connecting the emission condenser (see p. 22).

• Complete system: connecting the gel dryer (see p. 23)

• Basic device: connecting the vacuum pump (see p. 23).

4. Check that the mains voltage and mains frequency comply with the requirements on the name plate for the device.

5. Connect the device to the power supply and switch on with the mains power switch located on the right-hand side of the device (basic device: at the rear of the device) (refer to fold-out page at the front of the manual).

• Display is active.

• Lid is released (lid indicator lamp lights up).

• You can now open the lid.

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

4.3.2 Complete system: connecting the emission condenser

Risk of injury from overpressure.

Overpressure in the exhaust gas lines (e.g., from closed taps or blocked pipes) can cause the

WARNING!

Hint!

pipes to burst.

Please check whether the use of the emission condenser is sufficient for the intended application. For the evaporation of chemically aggressive or biologically hazardous substances one of the following measures must be taken:

• Replace the emission condenser with a suitable cold trap or chemical trap.

• Place the emission condenser in an ice bath.

• Attach a hose to the upper connection of the emission condenser and connect it to an

extraction system.

• Set up a series connection of cold trap or chemical trap and emission condenser.

The pump of Concentrator plus and/or Vacufuge plus is fully solvent resistant. Therefore, the

Hint!

emission condenser can be placed behind the pump.

1. Remove packaging from the emission condenser.

2. Remove red cap from the upper connection.

3. Screw the angle connection sideways into the emission condenser.

There must be a fall from the pump outlet to the emission condenser inlet to prevent liquid from collecting in the hose.

4. Connect the pump outlet with the connection on the side of the emission condenser using the hose.

5. Attach the emission condenser from the side or from the front below the front left foot of the device.

6. You can install a filter at the upper connection of the emission condenser. The overpressure at the pump outlet of the complete system must not exceed 1 bar.

Concentrator plus/Vacufuge® plus — Operating manual

4.3.3 Complete system: connecting the gel dryer

Requirement

• 1 connecting hose (length: max. 50 cm, inner diameter: 5 to 7 mm, chemical resistant and

suitable for vacuum applications).

1. Screw the hose connection supplied (size: G1/4 ") into the thread of the stop valve on the left-hand side of the device.

2. Connect the gel dryer to the hose connection, using the connecting hose.

4.3.4 Basic device: connecting the vacuum pump

Danger of explosion from gas mixtures.

When running several devices in parallel on a vacuum pump and/or in combination with a gel

WARNING!

dryer, an explosive gas mixture can develop in the vacuum system.

 Note the solvent properties and only carry out parallel operations with identical solvents or

solvents that evaporate in a non-hazardous manner.

You can connect the basic device to an external vacuum pump.

Operating manual

Requirement

• The vacuum pump is approved according to the standards of your country.

• A separator (e.g., emission condenser, cold trap or chemical trap) between the devices or

behind the vacuum pump, depending on the application.

• Pump maintains an ultimate pressure of at least 20 mbar. Its suction capacity is at least 1.8

m³/h.

Vacuum pumps that meet these requirements, can, for example, be diaphragm or vane-type

Hint!

rotary pumps. The specifications can be found in the technical data of the vacuum pump or requested from the manufacturer. The manufacturer is also the contact for any further questions regarding the correct handling of the vacuum pump, e.g. operation, use, performance, maintenance, troubleshooting or the specifications and connection of a cold trap.

Please contact Technical Service if problems occur when connecting a vacuum pump. You can find the contact addresses at the end of the operating manual or on the Internet at www.eppendorf.com

Concentrator plus/Vacufuge® plus — Operating manual

Connecting vacuum pumps up to 350 W

Requirement

• Power consumption of the vacuum pump: max. 350 W or electrical connection value max.

400 VA.

• Special plug for control and power supply of the vacuum pump (see Accessories on p. 43)

• 1 connecting hose (length: max. 50 cm, inner diameter: 8 mm, chemical resistant and suitable

for vacuum applications).

Abb. 2: Special plug for control and power supply of a vac uum pump with a power consumption of up to 350 W.

Operating manual

Fig. 2: Special plug for control and power supply of a vacuum pump with a power

consumption of up to 350 W.

Abb. 3: Connection of the power consumption for a vacuum pump up to 350 W to th e Concentrator plus basic device.

Fig. 3: Connection of the power consumption for a vacuum pump up to 350 W to the

Concentrator plus basic device.

1. Disconnect the basic device 1 and vacuum pump 5 from the mains power supply.

2. Insert the mains cable of the vacuum pump 6 into the special plug 7.

3. Insert the special plug into the provided socket 8 at the rear of the basic device (connected mains voltage!).

4. Connect the inlet of the vacuum pump 4 to the pump connection 2 at the rear of the basic device via a connecting hose 3.

5. Connect the basic device to the mains power supply 9.

4 5

6789

P A

4 5

891011

Concentrator plus/Vacufuge® plus — Operating manual

Connect vacuum pump with external power supply

Requirement

• Additional power source for the vacuum pump.

• Solenoid valve for controlling the vacuum pump via the basic device (see Accessories on

p. 43)

• 2 connecting hoses (length: max. 50 cm, inner diameter: 8 mm, chemical resistant and

suitable for vacuum applications)

Abb. 4: Solenoid valve for controlling a vacuum pump with external power supply.

Fig. 4: Solenoid valve for controlling a vacuum pump with external power supply.

Abb. 5: Connecting a vacuum pump w ith external power supply to the Concentrator plus basic device.

P A

Operating manual

Fig. 5: Connecting a vacuum pump with external power supply to the Concentrator plus basic

device.

1. Disconnect the basic device 1 and the vacuum pump 7 from the mains power supply.

Hint!

2. Connect the hose connection P of the solenoid valve 4 to the pump connection 2 at the rear of the basic device via a connecting hose 3.

3. Connect the hose connection A of the solenoid valve 4 to the inlet of the vacuum pump 6 via a connecting hose 5.

4. Insert the plug of the solenoid valve 9 into the provided socket 10 at the rear of the basic device (connected mains voltage!).

5. Connect the basic device to the mains power supply 11.

6. Connect the vacuum pump 8 to the mains power supply.

The vacuum pump is isolated from the device automatically before concentration is complete by closing the solenoid valve which is piloted by the concentrator. The isolation can also be carried out without a solenoid valve by closing an in-line valve manually (e.g., stop valve with a ground-in stopper).

Concentrator plus/Vacufuge® plus — Operating manual

5 Operation

5 Opera tion

5.1 Overview of operating controls

Before using the device for the first time, familiarize yourself with the operating controls and the device display. A depiction of the operating controls and device display can be found on the fold-out page (see Fig. C).

Operating manual

1 Concentration run time

1 min to 9:59 h, infinitely (oo), adjustable in 1 min increments.

3 Temperature

--: Heater off. 30/45/60 °C: Heater on.

5 Status of the diaphragm vacuum pump (vac)

Off: Pump off. On: Pump running.

7 Starting and/or stopping concentration 8 Setting the mode

9 Setting the temperature 10 Setting the braking function

11 Setting the concentrator run time

2 Braking function

ON: Braking function on. OFF: Braking function off.

4Mode

(see Functions on p. 26)

6 Status of the device lid (lid)

Off: Lid locked. On: Lid unlocked.

Manual ventilation of the rotor chamber during a concentrator/desiccator run while the key is pressed and held. Ventilation is displayed.

5.2 Functions

Concentrator plus and Vacufuge plus can be used as a concentrator, a desiccator or a centrifuge. For the concentrator and desiccator functions, there are three different modes available for the quick concentration of different solvents:

Function Mode Suitable for

Concentrator V-AQ (vacuum - aqueous) aqueous solutions

V-AL (vacuum - alcoholic) alcoholic solutions

V-HV (vacuum - high vapor) solutions with high vapor pressure

Desiccator D-AQ (desiccator - aqueous) aqueous solutions

D-AL (desiccator - alcoholic) alcoholic solutions

D-HV (desiccator - high vapor) solutions with high vapor pressure and dry substances

Centrifuge CEFU (centrifuge)

centrifugation at 1,400 min

 To set the desired function, press the mode/vent key before operation of the device.

Use the V-AQ, V-AL, D-AQ or D-AL modes if possible, or press the mode/vent key from time to

Hint!

time during the run.

During operation, you cannot change the set function. Instead, holding the mode/vent key during the concentrator or desiccator function ventilates the rotor chamber.

-1

Concentrator plus/Vacufuge® plus — Operating manual

5.3 Preparation for concentration

5.3.1 Switching on the device

Please note before putting the device into service that the ambient temperature during operation

Hint!

5.3.2 Inserting the rotor

CAUTION!

must be between 15 and 35 °C. At altitudes above 1000 m MSL (risk of insufficient cooling air supply), measures in accordance

with DIN EN 60034-1; VDE 0530-1 are required.

 Switch on the device at the mains power switch.

• Display is active.

• Lid is released (lid indicator lamp lights up).

• You can now open the lid.

• The parameter settings of the last run are displayed.

Risk of injury from chemically or mechanically damaged accessories.

Even small scratches and cracks can lead to serious internal material damage.

 Protect all parts from mechanical damage.  Check accessories regularly.  Do not use rotors or buckets with signs of corrosion or mechanical damage (e.g.

deformations).

 Do not use accessories whose maximum service life has been exceeded.  When inserting the buckets and rotors, ensure that they do not become scratched.

Operating manual

Hint!

5.3.3 Warm-up phase

WARNING!

First observe the rotor information (see p. 11) and the special notes on rotors F-35-6-30, F-45-72-8, F-45-48-11, F-45-24-12 and A-2-VC (see p. 13).

Rotor A-2-VC: remove the buckets before inserting the rotor and pick it up at the rotor cross, using both hands.

Proceed as follows when inserting the rotor:

1. Place rotor on the motor shaft.

2. Push rotor down using slight pressure until you encounter resistance.

The rotors do not need to be screwed in.

The diaphragm vacuum pump reaches the displayed output and the ultimate pressure (see p. 41) only after the device has gone through a 15 minute warm-up phase. The warm-up phase reduces the condensation of liquid in the pump and on the lid of the rotor chamber and therefore prolongs the service life of the pump.

Device lid can crush. Keep hands clear.

 Do not reach between the device and lid when opening or closing the device lid.

1. time arrow keys: set time to 15 minutes.

2. brake: switch brake on or off.

3. temp: select temperature.

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

4. mode/vent: select V-AQ, V-AL or V-HV mode.

5. Close the lid of the device.

6. Press start/stop to start the warm-up phase.

• The device lid is locked, and the green lid indicator lamp goes out.

• The rotor starts up.

• At 1,000 rpm, the vacuum pump switches on, and the vac indicator lamp lights up.

• The vent valve is closed.

• The rotor accelerates to the end point of 1,400 rpm.

• On the display, the colon of the time display flashes while the rotor is turning.

• The remaining run time is displayed in hours and minutes.

After the warm-up phase is completed

• The device stops automatically.

• During braking the elapsed concentration time is displayed flashing.

• The rotor chamber is ventilated so that the pressure in the chamber increases slowly.

• After two seconds, the vacuum pump is switched off, and the vac indicator lamp goes out.

• Then the device brakes.

• When the rotor has come to a standstill, the lid indicator lamp lights up.

• The lid can be opened.

5.3.4 Loading a fixed-angle rotor

The following notes apply to fixed-angle rotors. Loading the rotor A-2-VC is described in the following chapter (see Loading a swing-bucket rotor on p. 29).

Risk of injury from unsymmetric loading of rotors.

 Load rotors symmetrically with identical tubes and/or buckets and plates.

CAUTION!

evaporation. Otherwise an imbalance can occur and the concentration may automatically stop.

 Check for symmetric loading by balancing the adapters and tubes and/or plates used with

scales.

The device automatically detects imbalances during operation and stops the run immediately with an error message and a signal tone. Check the load, balance the tubes and restart the run.

Risk from damaged or overloaded tubes!

 When loading the rotor, note the safety instructions with regard to risks from overloaded or

damaged tubes (see Warnings for intended use on p. 15)

Proceed as follows when loading the fixed-angle rotor:

1. Check the maximum payload (tube and content) per rotor bore. Detailed information can be found in this operating manual (see Rotors on p. 11).

2. Only load rotors with the tubes intended for this purpose.

3. Insert open tubes opposite each other in pairs into the rotor bores. For symmetrical loading, tubes that are opposite each other must be of the same type and contain the same filling quantity.

In order to minimize weight differences between filled sample tubes, we recommend taring with a scale. This will reduce wear on the drive and cut running noise.

Concentrator plus/Vacufuge® plus — Operating manual

5.3.5 Loading a swing-bucket rotor

Requirement

• A combination of rotor, bucket and adapter, approved by Eppendorf.

• Two inserted buckets.

• Matching and tested tubes and plates.

• Adapters and plates with a total height of  27 mm

Risk of injury from unsymmetric loading of rotors.

 Load rotors symmetrically with identical tubes and/or buckets and plates.

CAUTION!

evaporation. Otherwise an imbalance can occur and the concentration may automatically stop.

 Check for symmetric loading by balancing the adapters and tubes and/or plates used with

scales.

The device automatically detects imbalances during operation and stops the run immediately with an error message and a signal tone. Check the load, balance the tubes and restart the run.

Operating manual

Filling the plates too high can cause overflowing.

During the run the meniscuses in the tubes along the edges of the plates are at an angle. This is

NOTICE!

5.3.6 Closing the lid of the device

due to the centrifugal forces and cannot be avoided.

 Fill the wells of the plates to a maximum of 2/3 of the max. filling volume.

1. Check the bucket grooves for cleanliness and grease lightly with pivot grease (order no. int.: 5810 350.050 / North America: 022634330).

Dirty grooves and pivots prevent buckets from swinging out evenly.

2. Hang the buckets into the rotor.

3. Check that both buckets are hanging properly and can swing freely.

4. When using a plate type for the first time, carry out a manual loading and swing-out test.

5. Check maximum payload (adapter, plate and content) per bucket. The relevant details can be found on the rotor and in this operating manual (see Rotors on

p. 11).

6. Load the buckets symmetrically when inserting adapters and plates.

The plate arrangement shown on the right-hand side is incorrect, as the buckets will not swing properly.

The plates have some play in the buckets.

WARNING!

Device lid can crush. Keep hands clear.

 Do not reach between the device and lid when opening or closing the device lid.

1. Check that the rotor is correctly positioned.

2. Close the lid of the device.

Concentrator plus/Vacufuge® plus — Operating manual

5.4 Concentration

The prerequisite for all applications described here is the previously described preparation (see Preparation for concentration on p. 27).

Risk of injury from improperly attached rotors.

 Only operate the device if the rotor has been mounted completely.

WARNING!

 If there are any unusual noises when the device is started up, immediately press the start/

stop key to stop concentrating.

Operating manual

Risk from incorrectly loaded rotors and damaged/overloaded tubes!

 Before commencing concentration, follow the safety instructions relating to risks from

CAUTION!

NOTICE!

Hint!

5.4.1 Concentration with time setting

unsymmetrically loaded and/or overloaded rotors and from overloaded and/or damaged tubes (see Warnings for intended use on p. 15).

Overflowing of the emission condenser.

The maximum filling volume of the emission condenser is 280 mL.

1. Check before each run whether the volume of the emission condenser is sufficient for your application.

2. Empty the emission condenser, if required (see Emptying the emission condenser on p. 32).

Unusual noises may occur when braking heavy rotors. These noises are due to the design and do not indicate problems.

Perform the following steps in the sequence described.

1. time arrow keys: set run time.

2. brake: switch brake on or off.

3. temp: select temperature (observe temperature resistance of the samples).

4. mode/vent: select V-AQ, V-AL or V-HV mode.

5. start/stop: start concentration.

• The device lid is locked, and the green lid indicator lamp goes out.

• The rotor starts up.

• At 1,000 rpm, the vacuum pump switches on, and the vac indicator lamp lights up.

• The vent valve is closed.

• The rotor accelerates to the end point of 1,400 rpm.

• On the display, the colon of the time display flashes while the rotor is turning.

• The remaining run time is displayed in hours and minutes.

Concentrator plus/Vacufuge® plus — Operating manual

5.4.2 Concentration with continuous run

Continuous delivery can affect safe operation of the device.

When delivering liquids continuously, membranes and valves of the vacuum pump can be

CAUTION!

Hint!

5.4.3 During or after concentration

damaged.

 Only use the device for applications of limited duration.

Use the continuous run function if you do not want to set a fixed run time.

1. Use the time arrow keys to set the continuous run function (oo), reachable under 0:01 or over 9:59.

2. Set all other parameters as previously described (see Concentration with time setting on p. 30).

3. Press start/stop to start concentration. Time counting is upwards in minute increments.

4. Press start/stop to end the concentration after the desired time.

If the device runs longer than 9:59 h, 9:59 remains on the display.

During the run you can:

• Time setting: change the total run time. The new parameters are adopted immediately. Note

that the shortest new total run time which can be set is the time which has already elapsed plus 2 minutes.

• Adjust the setting of the braking function and the temperature.

• Ventilate the rotor chamber manually by holding the mode/vent key. In this way, condensation

can be removed from the device lid and the pump and the hose system rinsed.

• Check by means of a stroboscope how far the concentration has progressed.

• Stop concentration before the set run time has elapsed. To do so, press the start/stop key.

Operating manual

When concentration is completed

• The device stops automatically (time setting) or manually (continuous run).

• During braking the elapsed concentration time is displayed flashing.

• The rotor chamber is ventilated so that the pressure in the chamber increases slowly.

• After two seconds, the vacuum pump is switched off, and the vac indicator lamp goes out.

• Then the device brakes.

• When the rotor has come to a standstill, the lid indicator lamp lights up.

• The lid can be opened and the samples removed.

If a temperature has been set, the rotor chamber is permanently heated, i.e. even when the rotor

Hint!

5.4.4 Completing an application

is at a standstill and the lid is open. Therefore switch the device off after the application or set the temperature to - - (no temperature set), if applicable.

1. After the end of the application, leave the device running in D-AQ mode for 15 min with an empty rotor.

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

5.4.5 Removing the rotor and switching off the device

Rotor A-2-VC: Remove the buckets first before holding the rotor at the rotor cross with both

Hint!

5.4.6 Emptying the emission condenser

hands to lift it out of the device.

1. If required, switch off the heater.

2. Remove the rotor.

3. Wipe up any liquid that has been spilled in the rotor chamber and on the device lid, using an absorbent cloth.

4. Clean the rotor chamber and device lid, as described separately (see Cleaning / Disinfection on p. 35).

5. Leave the device lid open and secure it against falling shut to let any remaining residual liquid evaporate.

6. Switch off the device at the mains power switch.

1. Depending on the design, there are two options:

• The emission condenser is mounted to the side of the device: detach the tube from

the connection and empty the liquid into a collection vessel via the upper connection.

• The emission condenser is mounted to the front of the device: here you can

alternatively remove the black plug in the vessel using a screwdriver and fit a special tap before first use. Via this tap, you can then empty the liquid directly into a collection vessel.

2. Dispose of the liquid in accordance with the current legal requirements and regulations for your application.

5.5 Notes on using rotors

5.5.1 RotorA-2-VC

Transferring the rotor

Wait for rotor to stop.

When the plates are fully loaded, it can happen during the post-run phase of the A-2-VC rotor that

CAUTION!

NOTICE!

the lid of the device can be opened before the rotor has stopped.

 Always wait until the rotor has stopped before opening the lid of the device and removing the

plates and/or tubes.

If handled incorrectly, the rotor can fall over.

The buckets of swing-bucket rotors must not be used as handles.

 Before moving the rotor, remove the buckets.  Always pick up the rotor at the rotor cross, using both hands.

Concentrator plus/Vacufuge® plus — Operating manual

5.6 Special function

5.6.1 Operation as a desiccator

With the desiccator function the rotor chamber is evacuated. However, the rotor is not turned. You can place the sample tubes directly in the rotor chamber without a rotor or insert them into a

rotor.

1. mode/vent: select the D-AQ, D-AL or D-HV mode.

2. Then proceed as with the concentrator function (see Concentration on p. 30).

5.6.2 Operation with gel dryer

On a complete system with a gel dryer connection, a gel dryer can be run in parallel to the operation as a concentrator and/or desiccator or used individually.

Danger of explosion from gas mixtures.

When running several devices in parallel on a vacuum pump and/or in combination with a gel

WARNING!

dryer, an explosive gas mixture can develop in the vacuum system.

 Note the solvent properties and only carry out parallel operations with identical solvents or

solvents that evaporate in a non-hazardous manner.

Operating manual

1. mode/vent: select the V-AQ, V-AL, V-HV, D-AQ, D-AL or D-HV mode. We recommend the V-HV and D-HV modes.

If you use the gel dryer individually, you should use the D-AQ, D-AL or D-HV modes in order to protect the drive.

2. In contrast to the concentrator or desiccator function, open the tap of the gel dryer connection before operation ( knob points in flow direction) and close it again after operation.

When the gel dryer is operated without concentrator or desiccator function the device lid must also be closed as a vacuum cannot be generated otherwise.

3. Then proceed as with the concentrator function (see Concentration on p. 30).

5.6.3 Operation as a centrifuge

With the centrifuge function, the rotor is turned. However, the rotor chamber is not evacuated.

1. mode/vent: select the CEFU mode.

2. Then proceed as with the concentrator function (see Concentration on p. 30).

As no vacuum is applied in this operating mode, the mode/vent key does not have any function during operation.

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

6 Maintenance

6 Mai ntenance

6.1 Maintenance

6.1.1 Device

 Avoid the drying of saline, aqueous solutions on the material, and avoid long-term exposure

of the material (aluminum) to acids and alkalis.

 Avoid the use of aggressive chemicals, including strong and weak alkalis, strong acids,

solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons, concentrated saline solutions and phenol.

 For the frequent evaporation of corrosive liquids, apply a thin coat of pivot grease to the rotor

and rotor chamber (order no. int.: 5810 350.050 / North America: 022634330).

6.1.2 Pump

The chemical-resistant pump of the complete system does not need to be maintained by the user. However, the valves and diaphragms are subject to natural wear and tear.

 Regularly remove the condensation from the pump and the hose system. To do so, complete

a 15 min run in D-AQ mode without samples. This will prolong the service life of the consumables.

 Observe any changes in the time required for your application. If you notice any deterioration,

have the valves and diaphragms checked by the authorized service.

6.2 Prepare cleaning / disinfection

Clean all accessible surfaces of the device and the accessories at least weekly and when



contaminated.

 Clean the rotor regularly. This way the rotor is protected and the durability is prolonged.  Furthermore, observe the notes on decontamination (see Decontamination before shipping

on p. 36) when the device is sent to the authorized Technical Service for repairs.

The procedure described in the following chapter applies to the cleaning as well as to the disinfection or decontamination. The table below describes the steps required on top of this:

Cleaning Disinfecting / decontamination

1. Use a mild cleaning fluid to clean the accessible surfaces of the device and the accessories.

2. Carry out the cleaning as described in the following chapter.

If you have any further questions regarding the cleaning and disinfection or decontamination or

Hint!

regarding the cleaning fluid to be used, contact the Eppendorf AG Application Support. The contact details are provided on the back of this manual.

1. Choose the disinfection method which corresponds to the legal regulations and guidelines in place for your range of application. For example, use alcohol (ethanol, isopropanol) or alcohol-based disinfectants.

2. Carry out the disinfection or decontamination as described in the following chapter.

3. Then clean the device and the accessories.

Concentrator plus/Vacufuge® plus — Operating manual

6.3 Cleaning / Disinfection

DANGER!

NOTICE!

Electric shock as a result of penetration of liquid.

 Switch off the device and disconnect it from the power supply before starting cleaning or

disinfecting work.

 Do not allow any liquids to penetrate the inside of the housing.  Do not perform spray disinfection on the housing.  Only reconnect the device to the power supply once it is completely dry.

Damage from the use of aggressive chemicals.

 Do not use any aggressive chemicals on the device or its accessories, such as strong and

weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.

 If the device becomes contaminated with aggressive chemicals, clean it immediately with a

mild cleaning agent.

Corrosion from aggressive cleaning agents and disinfectants.

 Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes.  Do not incubate the accessories in aggressive cleaning agents or disinfectants for prolonged

periods.

Damage from UV and other high-energy radiation.

 Do not use UV, beta or gamma radiation or any other type of high-energy radiation for

disinfecting.

Operating manual

Autoclaving

Hint!

6.3.1 Cleaning / Disinfecting the device

All rotors, buckets and adapters can be autoclaved (121 °C, 20 min).

1. Switch off the device at the mains power switch while the lid is open, and disconnect the power plug from the power supply.

2. Remove rotor.

3. Use the agents specified above (see p. 34) to clean and disinfect the device and the rotor chamber.

4. Wipe all accessible surfaces of the device and accessories including the mains cable with a damp cloth.

5. Thoroughly clean the rubber seals of the rotor chamber with water.

6. Rub the dry rubber seal with glycerine or talcum powder to prevent it from becoming brittle. Other components of the device, such as the motor shaft and rotor cone, must not be lubricated.

7. Clean the motor shaft with a soft, dry and lint-free cloth.

8. Check device and accessories for corrosion and damage.

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

6.3.2 Cleaning / Disinfecting the rotor

1. Clean rotors, buckets and adapters with the agents mentioned above and disinfect them (see p. 34).

2. Rinse rotors, buckets and adapters thoroughly with water.

3. Place rotors and accessories onto a cloth to dry.

4. Clean the rotor cone with a soft, dry and lint-free cloth and inspect for damage. Do not lubricate the rotor cone.

5. Place the dry rotor onto the motor shaft.

6. Load the fixed-angle rotor with the cleaned adapters or the swing-bucket rotor with the cleaned buckets and adapters, if necessary.

6.4 Glass breakage

Note when using glass tubes that the danger of breakage of glass increases with an increasing

Hint!

g-force (rcf)/speed (rpm). Please note the manufacturer's information on the recommended centrifugation parameters (load and speed).

Broken glass scratches the surfaces of the rotor chamber and the accessories (rotors, buckets and adapters) so that their chemical resistance is reduced. Therefore, a fine, black metal abrasion develops in the rotor chamber due to the air turbulence which, in addition to causing damage to the rotor chamber and the accessories, can also contaminate the samples.

 Carefully remove all splinters and glass powder from the rotor chamber and accessories

(rotors, buckets and adapters) if breakage of glass occurs.

 If required, replace the adapters to prevent any further damage.  Check the rotor regularly for residues or damage.

6.5 Replacing fuses

The fuse holder is located between the mains connection socket and the mains power switch.

1. Disconnect the power plug.

2. Press the upper and lower end of the plastic springs 1 together and pull the fuse holder 2 fully out.

3. Replace faulty fuses and reinsert the fuse holder. Make sure that the guiding rail 3 is positioned correctly.

6.6 Decontamination before shipping

If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following:

Risk to health from contaminated device.

1. Follow the instructions in the decontamination certificate. It is available in PDF format on our

WARNING!

homepage (www.eppendorf.com/decontamination)

2. Decontaminate all the parts you want to dispatch.

3. Enclose the fully-completed decontamination certificate for returned goods (including the serial number of the device) with the dispatch.

Concentrator plus/Vacufuge® plus — Operating manual

7 Troubleshooting

7 Troubleshootin g

If the suggested measures fail repeatedly, please contact Technical Service. You can find the contact addresses at the end of this operating manual or on the Internet under www.eppendorf.com

7.1 General errors

Symptom / message Cause Remedy

No display. No mains connection.

No display. Power failure.

 Check the mains connection.

 Check the mains fuse for the device

(see Replacing fuses on p. 36).

 Check the mains fuse for the laboratory.

Lid of the device cannot be opened.

Device cannot be started. Lid of the device is not closed.  Close the lid of the device.

Device shakes when it starts up.

No noticeable evaporation.

Rotor is still running.

Power failure. 1. Check the mains fuse for the device

Rotor is unsymmetrically loaded. 1. Stop the device and load symmetrically.

Damaged sealing ring in the lid of the device.

 Wait for rotor to stop.

(see Replacing fuses on p. 36).

2. Check the mains fuse for the laboratory.

3. Activate the emergency lid release (see p. 38).

2. Restart the device.

 Insert new sealing ring (see Other

accessories on p. 44).

Operating manual

Pump does not start up. Pump is overloaded.

Thermo switch in the motor winding of the pump triggered.

Pump does not start up. Overpressure in the exhaust gas line.

No output. Long, thin hoses.

No output. Condensation in the pump.

Temperature value flashes.

Temperature value flashes fast.

Liquid collects in the hoses.

Deviation from set value by ± 5 °C.

Deviation from set value by + 10 °C.

 Let the pump cool down.

 Open the exhaust gas line.

 Select short hoses with a large

cross-section.

 Let pump run for a few minutes and press

the mode/vent key several times.

 Let the remaining heat from the previous

run cool down.

 Reduce the excessive ambient

temperature, if applicable.

 Switch off the external heat radiators (e.g.

halogen lamp).

 Activate the ventilation function.  Check the fall of the hoses.

Concentrator plus/Vacufuge® plus — Operating manual

7.2 Error messages

If one of the following error messages appears, proceed as follows:

1. Remove fault (see Remedy).

2. Press the start/stop key to clear the error message.

3. If required, continue concentration and/or repeat.

Symptom / message Cause Remedy

Operating manual

Error 1 Drive fault

Error 2

Error 3

Error 4

Drive blocked or stiff.

 Contact Service.

 Move the rotor by hand, remove any

obstructions.

 Check the max. load of rotor.  Check the rotor mounting.

Error 5 Drive fault

Error 7 Error in the lid latch.

Error 8 Fault at the temperature sensor

Error 9 Electronics fault.

 Contact Service.

Caution! The rotor chamber

temperature may be > 72 °C.

 Contact Service.

Error 10 Heater defective.

Error 12

Error 14

Error 16

IMBAL Rotor unsymmetrically loaded.  Load the rotor symmetrically and balance.

Electronics fault.

 Contact Service.

7.3 Opening the device in case of a power failure

If the device lid cannot be opened during a power failure, you can activate the emergency lid release manually.

WARNING!

Hint!

Risk of injury from rotating rotor.

 Wait for the rotor to stop before activating the emergency lid release.

If there is a power failure, the vent valve will open. Before the rotor comes to a full stop, the standard pressure in the rotor chamber will be restored.

1. Disconnect the power plug.

2. Insert a wire (max. 2.5 mm thick, e.g., a paper clip) into the opening on the right-hand side of the housing (see point 5 in Fig. A and Fig. B on the fold-out page), and push against the noticeable resistance.

This will release the device lid.

3. Open the device lid slightly.

4. Remove the wire.

5. Open the device lid fully.

Concentrator plus/Vacufuge® plus — Operating manual

8 Transport, storage and disposal

8 Transport, stora ge and disposal

8.1 Transport

8.2 Storage

8.3 Disposal



Only transport the device in the original packaging.

Air temperature

General transportation -25 to 60 °C / 

-10 to 60 °C

Air freight -40 to 55 °C / 

-10 to 55 °C

) Basic device / complete system

Air temperature

Device in transport packaging

Device without transport packaging

) Basic device / complete system

-25 to 55 °C / 

-10 to 55 °C

-5 to 45°C 10 to 95% 70 to 106 kPa

Operating manual

Rel. humidity Atmospheric

pressure

10 to 95% 30 to 106 kPa

Rel. humidity Atmospheric

pressure

10 to 95% 70 to 106 kPa

Always observe the statutory requirements when disposing of the product.

Information on the disposal of electrical and electronic devices in the European Community:

Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, to the business-to-business area this product is assigned to, may no longer be disposed of in municipal or household waste. To document this, they have been marked with the following identification:

Because disposal regulations may differ from one country to another within the EU, please contact your supplier if necessary.

Concentrator plus/Vacufuge® plus — Operating manual

9 Technical data

9 Technical da ta

9.1 Power supply

5305 Basic device 5305 Complete system

Operating manual

Mains connection: 230 V, 50 to 60 Hz

Current consumption: 2.1 A (230 V)

Power consumption: max. 500 W (230 V)

Overvoltage category: II

Fuses: 4.0 AT (230 V)

9.2 Ambient conditions

Environment: For indoor use only.

Ambient temperature: 15 to 35 °C

Max. relative humidity: 75 %, non-condensing humidity

Atmospheric pressure: Use at altitudes up to 2000 m above MSL.

Degree of pollution: 2

120 V, 50 to 60 Hz 100 V, 50 to 60 Hz

6.0 A (120 V)

5.2 A (100 V)

max. 550 W (120 V) max. 520 W (100 V)

6.3 AT (120 V/100 V)

230 V, 50 to 60 Hz 120 V, 50 to 60 Hz 100 V, 50 to 60 Hz

1.7 A (230 V)

5.6 A (120 V)

3.8 A (100 V)

max. 350 W (230 V) max. 400 W (120 V) max. 380 W (100 V)

9.3 Weight / dimensions

Dimensions: Width: 330 mm (12.6 in.)

Weight without rotor: 16.5 kg (37.5 lbs.) 31.5 kg (68.3 lbs.)

Noise level:  50 dB(A)  50 dB(A)

5305 Basic device 5305 Complete system incl. gel

dryer

Width: 330 mm (12.6 in.) Depth: 372 mm (14.5 in.) Height: 231 mm (9.1 in.)

Depth: 579 mm (20.9 in.)

Height: 290 mm (11.7 in.)

Concentrator plus/Vacufuge® plus — Operating manual

9.4 Diaphragm vacuum pump for the complete system

Max. current: 1.4/1.6 A (230 V, 50/60 Hz)

3.0 A (120 V, 50 Hz)

3.6/3.8 A (100 V, 50/60 Hz)

Max. power: 180 W (230/120/100 V)

Motor protection: Thermal winding protection

Protection class according to IEC 529: IP 54

Suction capacity (in accordance with DIN EN 60529; VDE 0470-1):

Achievable ultimate pressure (absolute): <20 mbar

Max. permissible pressure at the outlet (absolute):

Max. differential pressure between inlet and outlet:

Nominal speed at 50/60 Hz: 1.500/1.800 rpm

Radio interference protection class B

Materials of surfaces that are in contact with the medium

Inner part of housing cover: PTFE, with carbon reinforcement

Head cover, diaphragm clamping disc: ETFE, with carbon reinforcement

1.9/2.1 m

2 bar

1 bar

/h (50/60 Hz)

Operating manual

Valve: FFKM

Diaphragm: PTFE

Inlet: ETFE

Outlet: ETFE

Screw connection: ETFE

Hose: PTFE

9.5 Application parameters

Run time: 1 min to 9:59 h, infinitely (oo),

Speed: 1,400 rpm, not adjustable

Max. relative centrifugal force (rcf):

Max. load: 96 micro test tubes of 2.0 mL each or

Permitted density of material for centrifuging (at max. rcf/speed and max. load):

Emission condenser volume: 280 mL

adjustable in 1 min increments.

248 x g, not adjustable

6 Falcon tubes of 50 mL each.

1.2 g/mL

Concentrator plus/Vacufuge® plus — Operating manual

10 Ordering Information

10.1 Complete system

Operating manual

Order No. (International)

5305 000.304 5305 000.312 5305 000.347

5305 000.509 5305 000.517 5305 000.541

5305 000.703 5305 000.711 5305 000.746

Order No. (North America)

5305000312

5305000517

5305000711

10.2 Basic device

Order No. (International)

5305 000.100 5305 000.118 5305 000.142

Order No. (North America)

5305000118

Description

Concentrator plus/Vacufuge plus complete system

with rotor F-45-48-11 230 V/50 - 60 Hz, with integrated diaphragm vacuum pump 120 V/50 - 60 Hz, with integrated diaphragm vacuum pump 100 V/50 - 60 Hz, with integrated diaphragm vacuum pump

Concentrator plus/Vacufuge plus complete system

without rotor 230 V/50 - 60 Hz, with integrated diaphragm vacuum pump 120 V/50 - 60 Hz, with integrated diaphragm vacuum pump 100 V/50 - 60 Hz, with integrated diaphragm vacuum pump

Concentrator plus/Vacufuge plus complete system

Description

Concentrator plus/Vacufuge plus basic device

with rotor F-45-48-11 230 V/50 - 60 Hz 120 V/50 - 60 Hz 100 V/50 - 60 Hz

10.3 Fuses

Order No. (International)

5301 850.249 5417 341.007

Order No. (North America)

022654403 022375831

Description

Fuse

2 x 4.0 A T (230 V) 2 x 6.3 A T UL (120 V / 100 V)

Concentrator plus/Vacufuge® plus — Operating manual

10.4 Accessories

10.4.1 Rotors

Order No. (International)

5490 034.007 022822080

5490 032.004 022822047

5490 030.001 022822004

5490 036.000 022822144

5490 041.003 022822233

5490 042.000 022822179

5490 038.002 022822225

5490 040.007 022822209

5490 035.003 022822128

5490 043.006 022822136

5490 037.006 022822161

5490 044.002 022822217

5490 039.009 022822187

5490 047.001 022822231

5490 045.009 022822241

Order No. (North America)

Description

Rotor F-45-72-8

72 positions for 0.5 mL tubes

Rotor F-45-70-11

70 positions for 1.5/2.0 mL tubes

Rotor F-45-48-11

48 positions for 1.5/2.0 mL tubes

Rotor F-45-24-12

24 positions for 6.0/8.0 mL round-bottom tubes (12 × 67-100 mm)

Rotor F-50-8-16

8 positions for 15.0/20.0 mL round-bottom tubes (16 × 105-120 mm)

Rotor F-50-8-18

8 positions for 15.0/20.0 mL round-bottom tubes (18 x 105-128 mm)

Rotor F-45-8-17

8 positions for 15 mL Falcon tubes (17 x 118-123 mm)

Rotor F-40-36-12

36 positions for 1.5 mL flat-bottom tubes (12 x 32 mm)

Rotor F-45-36-15

36 positions for 3.0/5.0 mL flat-bottom tubes (15 x 48 mm)

Rotor F-45-16-20

16 positions for 6.5/10.0 mL flat-bottom tubes (20 x 42-55 mm)

Rotor F-40-18-19

18 positions for 10.0 mL flat-bottom tubes (19 x 66 mm)

Rotor F-45-12-31

12 positions for 20.0 mL flat-bottom tubes (31 x 55 mm)

Rotor F-35-8-24

8 positions for 25.0 mL flat-bottom tubes (24 x 86-90 mm)

Rotor F-35-6-30

6 positions for 15 mL Falcon tubes (17 x 116-123 mm) and 50 mL Falcon tubes (29.5 x 116-123 mm)

Rotor A-2-VC

incl. 2 buckets

Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

10.4.2 Adapters

Operating manual

Order No. (International)

0030 124.235 951010031

0030 124.243 951010049

5825 706.005 022638963

5425 715.005 5425 717.008 5425 716.001

10.4.3 Other accessories

Order No. (International)

5301 316.005 022822101

5301 330.008 022830309

5301 337.002 022830295

5301 110.032 5301110032

5301 010.003 5301 033.003

5301 030.004 5301 036.002

5301 160.005 022830201

5810 350.050 022634330

5490 030.800 022830520

Order No. (North America)

022636260 022636243 022636227

Order No. (North America)

022830110

022830104 022830112

Description

Work tray

for 96 x 0.2_mL PCR tubes, 5-/8-PCR tube strips and plates without border set of 10

Frame for work tray

set of 5

Adapter

used in A-2-VC Combislide Adapter, set of 2

Adapter

used in F-45-48-11 and F-45-70-11 for 1 PCR tube (0.2 mL, max. Ø 6 mm), set of 6 for 1 sample tube (0.4 mL, max. Ø 6 mm), set of 6 for 1 sample tube (0.5 mL, max. Ø 6 mm) or 1 Microtainer (0.6 mL, max. Ø 8 mm), set of 6

Description

Spacer for simultaneous operation of two rotors

for F-45-72-8 and F-45-48-11

Emission condenser

without hose

Hose for emission condenser

length: 0.7 m

Tube connection

Plastic

Special plug for external vacuum pump < 400 W

230 V (Germany), different options upon request 120 V, different options upon request

Solenoid valve for external vacuum pump

Only suitable for aqueous and alcoholic solvents. 230 V, 50 - 60 Hz 120 V

Sealing ring

for lid

Pivot grease

Tube 20 mL

Rotor feet for F-45-72-8 and F-45-48-11

Set of 3

Concentrator plus/Vacufuge® plus

In touch with life

Your local distributor: www.eppendorf.com/worldwide

Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com

Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA

Tel: +1 516 334 7500 · Toll free phone: +1 800-645-3050 · Fax: +1 516 334 7506 · E-mail: info@eppendorf.com

Application Support Europe: Tel: +49 1803 666 789 (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min)

support@eppendorf.com

North America: Tel: +1 800 645 3050 · E-mail: techserv@eppendorf.com

Asia Pacific: Tel: +60 3 8023 6869 · E-mail: support_asiapacific@eppendorf.com

Eppendorf Concentrator plus User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Operating manual

1 User instructions

1.1 Using this manual

1.2 Warning signs and hazard icons

1.2.1 Hazard symbols

1.2.2 Degrees of danger

1.3 Symbols used

1.4 Abbreviations used

2 Product description

2.1 Main illustration

2.2 Features

2.3 Delivery package

2.3.1 Complete system

2.3.2 Basic device

2.4 Rotors

2.4.1 Special notes on the individual rotors

3 Safety

3.1 Intended use

3.2 User profile

3.3 Application limits

3.3.1 Declaration concerning the ATEX directive (94/9/EC)

3.3.2 Maximum service life for accessories

3.4 Information on product liability

3.5 Warnings for intended use

3.5.1 Personal injury or damage to the equipment

3.5.2 Incorrect handling of the device

3.5.3 Incorrect handling of the rotors

3.5.4 Extreme strain on the sample tubes

3.5.5 Vacuum

3.6 Safety instructions on the device

4 Installation

4.1 Selecting the location

4.2 Preparing installation

4.2.1 Open the packaging

4.2.2 Lift the device out of the box

4.2.3 Complete system: removing the transport protection screw

4.2.4 Place the device upright

4.2.5 Check the delivery

4.3 Installing the instrument

4.3.1 General Installation

4.3.2 Complete system: connecting the emission condenser

4.3.3 Complete system: connecting the gel dryer

4.3.4 Basic device: connecting the vacuum pump

5 Operation

5.1 Overview of operating controls

5.2 Functions

5.3 Preparation for concentration

5.3.1 Switching on the device

5.3.2 Inserting the rotor

5.3.3 Warm-up phase

5.3.4 Loading a fixed-angle rotor

5.3.5 Loading a swing-bucket rotor

5.3.6 Closing the lid of the device

5.4 Concentration

5.4.1 Concentration with time setting

5.4.2 Concentration with continuous run

5.4.3 During or after concentration

5.4.4 Completing an application

5.4.5 Removing the rotor and switching off the device

5.4.6 Emptying the emission condenser

5.5 Notes on using rotors

5.5.1 RotorA-2-VC

5.6 Special function

5.6.1 Operation as a desiccator

5.6.2 Operation with gel dryer

5.6.3 Operation as a centrifuge

6 Maintenance

6.1 Maintenance

6.1.1 Device

6.1.2 Pump

6.2 Prepare cleaning / disinfection

6.3 Cleaning / Disinfection

6.3.1 Cleaning / Disinfecting the device

6.3.2 Cleaning / Disinfecting the rotor

6.4 Glass breakage

6.5 Replacing fuses