Eppendorf Combitube User Manual

Eppendorf ® Combitube
General information
The Eppendorf Combitube is an aspirating aid for the Repeater 4780 / plus / pro / stream or Xstream. Using the Combitube, liquids can be directly taken out of all original bottles whose diameter does not exceed 50 mm. The Combitube can remain in the bottle and can be sealed by inserting a Combitip / Combitip plus into its opening.
Application
– Shorten the tube, e. g. with
a scalpel, to the desired length. The tube should not touch the bottom of the bottle (Fig. 1).
– Hang the Combitube into
the reagent bottle.
– Clamp the enclosed
Combitip plus 25 ml* into
firmly
the Repeater and insert the tip into the opening of the Combitube (Fig. 2).
– Aspirate liquid by slowly
sliding the filling lever of the Repeater up to the stop.
Fig. 1
Fig. 2
Fig. 3
The first time this is carried out,
A
the Combitip is not completely filled. Pull the Combitip a little out of the Combitube’s opening and dispense the liquid through the opening into the chamber (Fig. 3). The Combitube has to be filled with liquid up to the "min" mark (1).
A
– Re-insert the Combitip
firmly into the opening and fill your Combitip as usual without forming any air bubbles.
*The Combitube can also be used with all other Combitips. If the Combitip volume is ≤ 5 ml, ensure that the chamber is filled up to the "min" mark.
min
1
Applicational limitations
not
concentrations. It is
suitable for organic solutions (e. g. chloroform, ether)!
Cleaning the Combitube
Emptying
– Remove the Combitube
from the liquid and hold over a waste container (Fig.
4).
– Wipe the tube of the
Combitube e. g. with a tissue.
– Hold the Combitube with
the chamber facing
Fig. 4
Then rinse 2 to 3 times with distilled water and leave to dry.
If necessary, the Combitube can be autoclaved (at 121 C°, 20 min).
Ordering information
Combitube (set of 2), 25 ml Combitip plus, incl. adapter 022261550
Eppendorf
®
and Eppendorf Combitip® plus are registered trademarks of Eppendorf AG
downwards so that part of the liquid can run out. Draw up a Combitip and insert the cone into the tube. Push the piston of the Combitip down so that the air column presses the remaining liquid out of the Combitube.
Your local distributor: www.eppendorf.com/worldwide
Eppendorf AG 22331 Hamburg · Germany Tel. +49 40 538 01-0
1
Fax +49 40 538 01-556 E-Mail: eppendorf@eppendorf.com
Eppendorf North America, Inc. One Cantiague Road · P.O. Box 1019 Westbury, N.Y. 11590-0207 · USA Tel. +1 516 334 7500 Toll free phone 800 645 3050 Fax +1 516 334 7506 E-Mail: info@eppendorf.com
Application Support
Europe, International: Tel. +49 1803 666 789 E-Mail: support@eppendorf.com
North America: Tel. 800 645 3050 ext. 2258 E-Mail: support_NA@eppendorf.com
Asia, Pacific: Tel. +603 8023 2769
E-Mail: support_AsiaPacific@eppendorf.com
Printed in Germany
eppendorf® is a registered trademarkH 0030 059.069-02/1207
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