Eppendorf Combitips advanced User Manual

r usevanced®

N)ns for use

Combitips advanced

Instructions for use

2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior

permission of the copyright owner.

Eppendorf

and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany.

Eppendorf, Multipette and Repeater, Combitips advanced, Biopur are registered trademarks of Eppendorf AG, Hamburg, Germany.

Registered trademarks are not marked in all cases with ™ or

in this manual.

0012 581.025-02/032013

Table of contents

Combitips advanced

English (EN)

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

1.2 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

1.3 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2 Overview of Combitips advanced with color codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.4 Compatible devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.5 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.6 Evaluation criteria . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.7 Resistance to chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

4 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.2 Assembling the Combitip advanced and Adapter advanced. . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.3 Inserting the Combitip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

4.4 Dispense liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5.1.1 Autoclaving the Adapter advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5.1.2 Autoclaving the Combitips advanced Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

6 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

6.1 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

6.2 Errors with the Multipette M4/Repeater M4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

6.3 Errors with the Multipette plus/Repeater plus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

6.4 Errors with the Multipette (X)stream/Repeater (X)stream . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.5 Adjustable dispensing volumes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

7 Purity grades and certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

7.1 Eppendorf purity grades . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

7.2 Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

8 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

8.1 Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

8.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

8.3 Multipette plus/Repeater plus. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

8.4 Multipette (X)stream/Repeater (X)stream. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

9 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

9.1 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Operating instructions

Combitips advanced

English (EN)

1 Operating instructions

1.1 Using this manual

 Please read these instructions for use completely before using the Combitips advanced for the first time.

Also read the operating manual of the dispenser used.

1.2 Symbols used

Symbol Meaning

 You are requested to perform an action.

• List.

Perform these actions in the sequence described.

References useful information.

1.3 Glossary

Adapter advanced

Connecting piece for the dispenser when using Combitips advanced 25 mL and 50 mL

Biopur

Eppendorf Biopur requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. Eppendorf

Biopur bacterial DNA, pyrogen, DNase and RNase. Consumables with the Biopur purity grade are controlled and certified by an external laboratory. Certificates are available for downloading from our webpage

also fulfills the requirements with regard to sterility, absence of ATP, PCR inhibitors, human and

is an Eppendorf AG purity level for consumables. Eppendorf Biopur® meets the

www.eppendorf.com.

Coding

The dispenser uses the Compitip coding to detect the volume of the Combitips.

Color code

The color code displays the volume.

Dispensing volume

Volume per dispensing step.

Operating instructions

Combitips advanced

English (EN)

Eppendorf Quality

Eppendorf Quality is an Eppendorf AG purity grade for consumables. Eppendorf Quality meets the requirements for standard products, e.g., precision, accuracy, wetting behavior and tightness.

Graduation

Incremental graduation of a range, a surface or a volume.

Maximum volume

The maximum volume that can be used for dispensing.

Nominal volume

The maximum dispensing volume of a Combitip in conjunction with the selected dispensing device. The term "nominal volume" comes from the ISO 8655 standard.

PCR clean

PCR clean is an Eppendorf AG purity grade for consumables. PCR clean meets the requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. PCR clean also meets the requirements with regard to absence of human DNA, DNase, RNase and PCR inhibitors. Consumables with the PCR clean purity grade are controlled and certified by an external laboratory. Certificates are available for downloading from our webpage

www.eppendorf.com.

Positive displacement principle

The liquid comes into direct contact with the Combitip piston during aspiration and dispensing. Unlike with a pipette, the liquid and piston are not separated by an air cushion. A small air bubble is visible at the piston during dispensing.

Random error

Precision. Describes how large the deviations of several measurements are from each other, if the same volume is measured several times.

Remaining stroke

Liquid reserve. The liquid which remains after all dispensing steps have been completed. You can discard the liquid of the remaining stroke or reuse it.

Reverse stroke

After aspiration, the piston is moved into a defined position. Liquid is dispensed during this piston movement. The reverse stroke is not a dispensing step.

Operating instructions

Combitips advanced

English (EN)

Sterile

Sterile Eppendorf AG purity grade for consumables. Sterile meets the requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. Sterile also meets the requirements with regard to sterility and freedom from pyrogens.

Systematic error

Accuracy. Describes how close the average value of several measurements of the same volume is to the actual value.

2 Product description

2.1 Main illustration

Product description

Combitips advanced

English (EN)

1=50µL

1 Dispensing volume with the manual dispenser

at selection dial position 1

2 Graduation

2,5 mL

5 Color code

6 Coding

7 Piston

3 US patent numbers

4 Maximum volume

2.2 Overview of Combitips advanced with color codes

0.1 mL 0.2 mL 0.5 mL 1 mL 2.5 mL 5 mL 10 mL 25 mL 50 mL

2.3 Features

Combitips advanced are disposable devices for aspirating and dispensing liquids according to the positive displacement principle. Combitips advanced are used in Eppendorf dispensers (e.g., Multipettes/ Repeaters) and are made up of a cylinder and a piston. Combitips advanced in sizes 25 mL and 50 mL require an Adapter advanced. Combitips advanced are available in various sizes, which are marked using a color code.

Product description

Combitips advanced

English (EN)

2.4 Compatible devices

The Combitips advanced can be used with the following Eppendorf dispensers:

Dispenser Dispensing range

Multipette 4780/Repeater 4780 2 μL – 5 mL

Multipette plus/Repeater plus 1 μL – 10 mL

Multipette pro/Repeater pro 1 μL – 50 mL

Multipette stream/Repeater stream 1 μL – 50 mL

Multipette Xstream/Repeater Xstream 1 μL – 50 mL

EDOS 5222 1 μL – 50 mL

2.5 Materials

NOTICE! Aggressive substances may damage dispensers, Combitips and accessories.

 Check the chemical resistance when using organic solvents or aggressive chemicals.

Combitip advanced Material

Cylinder Polypropylene (PP)

Piston 0.1 mL and 0.2 mL Polyethylene (PE) with glass fiber (GF)

Piston 0.5 mL to 50 mL Polyethylene (PE)

Adapter advanced Polybutylene terephthalate (PBT)

Product description

Combitips advanced

English (EN)

2.6 Evaluation criteria

The Combitips advanced can be used for the single dispensing of all chemicals which are included in the following tables.

■■■ Resistant

The chemical can be used.

■■ Limited resistance and/or suitable for limited use

The chemical can be used for a limited period of time. Dispensing must be performed soon after filling in order to ensure the tightness of the Combitips advanced and to avoid damage to the dispenser. Prolonged contact may negatively affect the error and the printing of the Combitips advanced may become discolored and detached.

■ Increased risk and/or increased wear

The chemical can only be used with utmost caution. Dispensing must be performed immediately after filling in order to ensure the tightness of the Combitips advanced and to avoid damage to the Combitips advanced and to the dispenser. Prolonged contact may negatively affect the error and the printing of the Combitips advanced may become discolored or detached.

+ 20 hidden pages