o
E
r usevanced®
N)ns for use
Combitips advanced
Instructions for use
®
Copyright
©
2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior
permission of the copyright owner.
®
Eppendorf
and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany.
Eppendorf, Multipette and Repeater, Combitips advanced, Biopur are registered trademarks of
Eppendorf AG, Hamburg, Germany.
®
Registered trademarks are not marked in all cases with ™ or
in this manual.
0012 581.025-02/032013
Table of contents
Combitips advanced
English (EN)
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.2 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.3 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Overview of Combitips advanced with color codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.4 Compatible devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.5 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.6 Evaluation criteria . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.7 Resistance to chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
®
3
4 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.2 Assembling the Combitip advanced and Adapter advanced. . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.3 Inserting the Combitip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.4 Dispense liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.1.1 Autoclaving the Adapter advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.1.2 Autoclaving the Combitips advanced Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
6 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
6.1 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
6.2 Errors with the Multipette M4/Repeater M4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
6.3 Errors with the Multipette plus/Repeater plus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
6.4 Errors with the Multipette (X)stream/Repeater (X)stream . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6.5 Adjustable dispensing volumes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7 Purity grades and certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7.1 Eppendorf purity grades . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7.2 Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
8 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
8.1 Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
8.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
8.3 Multipette plus/Repeater plus. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8.4 Multipette (X)stream/Repeater (X)stream. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
9 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
9.1 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Operating instructions
Combitips advanced
4
English (EN)
®
1 Operating instructions
1.1 Using this manual
Please read these instructions for use completely before using the Combitips advanced for the first time.
Also read the operating manual of the dispenser used.
1.2 Symbols used
Symbol Meaning
You are requested to perform an action.
1.
2.
• List.
Perform these actions in the sequence described.
References useful information.
1.3 Glossary
A
Adapter advanced
Connecting piece for the dispenser when using Combitips advanced 25 mL and 50 mL
B
Biopur
®
Eppendorf Biopur
requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. Eppendorf
®
Biopur
bacterial DNA, pyrogen, DNase and RNase.
Consumables with the Biopur purity grade are controlled and certified by an external laboratory.
Certificates are available for downloading from our webpage
also fulfills the requirements with regard to sterility, absence of ATP, PCR inhibitors, human and
is an Eppendorf AG purity level for consumables. Eppendorf Biopur® meets the
www.eppendorf.com.
C
Coding
The dispenser uses the Compitip coding to detect the volume of the Combitips.
Color code
The color code displays the volume.
D
Dispensing volume
Volume per dispensing step.
Operating instructions
Combitips advanced
English (EN)
E
Eppendorf Quality
Eppendorf Quality is an Eppendorf AG purity grade for consumables. Eppendorf Quality meets the
requirements for standard products, e.g., precision, accuracy, wetting behavior and tightness.
G
Graduation
Incremental graduation of a range, a surface or a volume.
M
Maximum volume
The maximum volume that can be used for dispensing.
N
®
5
Nominal volume
The maximum dispensing volume of a Combitip in conjunction with the selected dispensing device. The
term "nominal volume" comes from the ISO 8655 standard.
P
PCR clean
PCR clean is an Eppendorf AG purity grade for consumables. PCR clean meets the requirements for
standard products, e.g., precision, accuracy, wetting behavior, tightness. PCR clean also meets the
requirements with regard to absence of human DNA, DNase, RNase and PCR inhibitors.
Consumables with the PCR clean purity grade are controlled and certified by an external laboratory.
Certificates are available for downloading from our webpage
www.eppendorf.com.
Positive displacement principle
The liquid comes into direct contact with the Combitip piston during aspiration and dispensing. Unlike with
a pipette, the liquid and piston are not separated by an air cushion. A small air bubble is visible at the piston
during dispensing.
R
Random error
Precision. Describes how large the deviations of several measurements are from each other, if the same
volume is measured several times.
Remaining stroke
Liquid reserve. The liquid which remains after all dispensing steps have been completed. You can discard
the liquid of the remaining stroke or reuse it.
Reverse stroke
After aspiration, the piston is moved into a defined position. Liquid is dispensed during this piston
movement. The reverse stroke is not a dispensing step.
Operating instructions
Combitips advanced
6
®
English (EN)
S
Sterile
Sterile Eppendorf AG purity grade for consumables. Sterile meets the requirements for standard products,
e.g., precision, accuracy, wetting behavior, tightness. Sterile also meets the requirements with regard to
sterility and freedom from pyrogens.
Systematic error
Accuracy. Describes how close the average value of several measurements of the same volume is to the
actual value.
2 Product description
2.1 Main illustration
Product description
Combitips advanced
English (EN)
®
7
^
1=50µL
1
2
1 Dispensing volume with the manual dispenser
at selection dial position 1
2 Graduation
2,5 mL
3
4
5
7
6
5 Color code
6 Coding
7 Piston
3 US patent numbers
4 Maximum volume
2.2 Overview of Combitips advanced with color codes
^
0.1 mL 0.2 mL 0.5 mL 1 mL 2.5 mL 5 mL 10 mL 25 mL 50 mL
2.3 Features
Combitips advanced are disposable devices for aspirating and dispensing liquids according to the positive
displacement principle. Combitips advanced are used in Eppendorf dispensers (e.g., Multipettes/
Repeaters) and are made up of a cylinder and a piston. Combitips advanced in sizes 25 mL and 50 mL
require an Adapter advanced. Combitips advanced are available in various sizes, which are marked using a
color code.
Product description
Combitips advanced
8
English (EN)
®
2.4 Compatible devices
The Combitips advanced can be used with the following Eppendorf dispensers:
Dispenser Dispensing range
Multipette 4780/Repeater 4780 2 μL – 5 mL
Multipette plus/Repeater plus 1 μL – 10 mL
Multipette pro/Repeater pro 1 μL – 50 mL
Multipette stream/Repeater stream 1 μL – 50 mL
Multipette Xstream/Repeater Xstream 1 μL – 50 mL
EDOS 5222 1 μL – 50 mL
2.5 Materials
NOTICE! Aggressive substances may damage dispensers, Combitips and accessories.
Check the chemical resistance when using organic solvents or aggressive chemicals.
Combitip advanced Material
Cylinder Polypropylene (PP)
Piston 0.1 mL and 0.2 mL Polyethylene (PE) with glass fiber (GF)
Piston 0.5 mL to 50 mL Polyethylene (PE)
Adapter advanced Polybutylene terephthalate (PBT)
Product description
Combitips advanced
English (EN)
2.6 Evaluation criteria
The Combitips advanced can be used for the single dispensing of all chemicals which are included in the
following tables.
■■■ Resistant
The chemical can be used.
■■ Limited resistance and/or suitable for limited use
The chemical can be used for a limited period of time. Dispensing
must be performed soon after filling in order to ensure the tightness
of the Combitips advanced and to avoid damage to the dispenser.
Prolonged contact may negatively affect the error and the printing of
the Combitips advanced may become discolored and detached.
■ Increased risk and/or increased wear
The chemical can only be used with utmost caution. Dispensing must
be performed immediately after filling in order to ensure the tightness
of the Combitips advanced and to avoid damage to the Combitips
advanced and to the dispenser. Prolonged contact may negatively
affect the error and the printing of the Combitips advanced may
become discolored or detached.
®
9
10
Product description
Combitips advanced
English (EN)
®
2.7 Resistance to chemicals
Acids and bases Concentration in % Resistance
Ammonia solution 25 ■■■
Ammonia solution 2.0 ■■■
Acetic acid 96 ■■■
Acetic acid 12 ■■■
Caustic soda 20 ■■■
Caustic soda 4.0 ■■■
Perchloric acid 10 ■■■
Nitric acid 65 ■■
Nitric acid 6.3 ■■■
Hydrochloric acid 32 ■■■
Hydrochloric acid 3.6 ■■■
Sulfuric acid 96 ■■■
Sulfuric acid 16 ■■■
Trichloroacetic acid 40 ■■
Trichloroacetic acid 10 ■■■
Trifluoroacetic acid 100 ■■
Trifluoroacetic acid 10 ■■■
Organic solvents Concentration in % Resistance
Acetone ■■
Acetonitrile ■■■
Petroleum ether ■■
Chloroform ■■
Dichloromethane ■■
Diethyl ether ■■
Dimethyl sulfoxide 100 ■
Acetic acid ethyl ester ■■■
Ethanol 96 ■■■
Formaldehyde 37 ■■■
Isoamyl alcohol ■■
Isopropanol ■■■
Methanol ■■■
Phenol ■■
Carbon tetrachloride ■■
Toluol ■■
Xylol ■■
Safety
Combitips advanced
English (EN)
3 Safety
3.1 Intended use
Combitips advanced are intended to be used with a Multipette/Repeater or a EDOS 5222 for dispensing
liquids in the 1 μL – 50 mL volume range. In-vivo applications (applications in or on the human body) are
not permitted. The Combitips advanced may only be used by specialized staff who have been adequately
trained. The user must carefully read the instructions for use and the operating manual of the dispenser
used and become familiar with how the device works.
3.2 Warnings for intended use
WARNING! Damage to health due to infectious liquids and pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the national regulations,
the biological security level of your laboratory, the material safety data sheets, and the
manufacturer's application notes.
Wear personal protective equipment.
Follow the instructions regarding hygiene, cleaning and decontamination.
For complete instructions regarding the handling of germs or biological material in risk
group II or higher, please refer to the "Laboratory Biosafety Manual" (source: World Health
Organization, current edition of the Laboratory Biosafety Manual).
®
11
WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.
Wear personal protective equipment.
Observe the national regulations for handling these substances.
Observe the material safety data sheets and manufacturer's application notes.
NOTICE! Carry-over, contamination and incorrect dispensing results due to the incorrect
use of Combitips.
Combitips are intended for single use. Prolonged use can have a negative impact on
dispensing accuracy.
Only use Combitips once.
Do not use washed and/or autoclaved Combitips for dispensing.
NOTICE! Incorrect dispensing results due to evaporation.
If dispensing with an already filled Combitip is continued after a long waiting time, the next
dispensing step may have a slightly reduced dispensing volume due to evaporation!
If high trueness is required, this dispensing step should not be carried out.
12
Safety
Combitips advanced
English (EN)
WARNING! Personal injury and material damage from a bursting Combitip.
If stored for a long period of time, liquids can crystallize or solidify and thus block the outlet
opening. The Combitip advanced may burst during the process of dispensing.
Check the consistency of the liquid.
Dispense immediately after liquid aspiration.
®
4 Operation
4.1 Unpacking
To ensure maximum protection from carry-over, use Combitips advanced with Sterile and
Biopur grades of purity immediately after they have been removed from their packing.
3. Insert the Combitip into the dispenser.
Operation
Combitips advanced
English (EN)
1. Open the packing at the location indicated.
2. Insert the Combitip into the dispenser.
®
13
14
Operation
Combitips advanced
English (EN)
®
4.2 Assembling the Combitip advanced and Adapter advanced
Combitips advanced with a maximum volume of 0.1 mL – 10 mL can be used immediately. Combitips
advanced with a maximum volume of 25 mL and 50 mL can only be used with the corresponding Adapter
advanced. Adapter advanced and Combitips advanced have the same color code. The maximum volume is
also listed on the neck of the Adapter advanced.
NOTICE! Sensor damage due to damaged or worn adapter
Always put the adapter and Combitip together outside of the dispenser.
Do not used damaged or worn adapters.
Do not use adapters with damaged coding.
1. Place the adapter on the Combitip.
2. Tighten the adapter.
4.3 Inserting the Combitip
NOTICE! Device damage due to incorrect handling of the inserted Combitip
Insert the Combitip straight into the dispenser from below.
Do not rotate the inserted Combitip.
Never grip the dispenser using the Combitip.
The Combitip is easier to insert if the ejector is held pressed down during insertion.
1 2
Operation
Combitips advanced
English (EN)
1. If the Combitip piston has been pushed out, push
it back into the cylinder of the Combitip.
2. Use force to push the Combitip into the opening
on the lower side of the dispenser until the
Combitip engages .
2
1
®
15
4.4 Dispense liquid
The liquid dispensing angle should always be as steep as possible. A dispensing angle greater
than 45° can result in an incorrect dispensing volume during the final dispensing steps.
Additional information can be found in the operating manual of your dispenser.
16
Maintenance
Combitips advanced
English (EN)
®
5 Maintenance
5.1 Cleaning
Combitips advanced are not autoclavable. Adapter advanced and Combitips advanced Rack
are autoclavable.
5.1.1 Autoclaving the Adapter advanced
Rinse the Adapter advanced with water.
Autoclave the Adapter advanced for 20 min at an overpressure of 1 bar and 121 °C. The Adapter
advanced can be autoclaved up to 100 times.
5.1.2 Autoclaving the Combitips advanced Rack
Autoclave the empty Combitips advanced Rack for 20 min at an overpressure of 1 bar and 121 °C.
Empty Combitips advanced Rack can be autoclaved up to 100 times.
6 Technical data
6.1 Ambient conditions
Ambience For indoor use only.
Ambient temperature 4 °C – 40 °C
Relative humidity 10 % – 95 %, non-condensing
Atmospheric pressure 79.5 kPa – 166 kPa
6.2 Errors with the Multipette M4/Repeater M4
Combitip advanced Testing volume Error limits
Error
Systematic error Random error
± %± µL ± %± µL
0.1 mL
white
Increment:
1 μL
0.2 mL
light blue
Increment:
2 μL
0.5 mL
purple
Increment:
5 μL
1 mL
yellow
Increment:
10 μL
2.5 mL
green
Increment:
25 μL
5 mL
blue
Increment:
50 μL
10 mL
orange
Increment:
0.1 mL
25 mL
red
Increment:
0.25 mL
50 mL
light gray
Increment:
0.5 mL
2 μL ±1.6 ±0.032 ±3.0 ±0.06
20 μL ±1.0 ±0.2 ±2.0 ±0.4
4 μL ±1.3 ±0.052 ±2.0 ±0.08
40 μL ±0.8 ±0.32 ±1.5 ±0.6
10 μL ±0.9 ±0.09 ±1.5 ±0.15
100 μL ±0.8 ±0.8 ±0.6 ±0.6
20 μL ±0.9 ±0.18 ±0.9 ±0.18
200 μL ±0.6 ±1.2 ±0.4 ±0.8
50 μL ±0.8 ±0.4 ±0.8 ±0.4
500 μL ±0.5 ±2.5 ±0.3 ±1.5
100 μL ±0.6 ±0.6 ±0.6 ±0.6
1 000 μL ±0.5 ±5.0 ±0.25 ±2.5
200 μL
0.2 mL
2 000 μL
2 mL
500 μL
0.5 mL
5 000 μL
5 mL
1 000 μL
1 mL
10 000 μL
10 mL
±0.5 ±1.0 ±0.6 ±1.2
±0.5 ±10 ±0.25 ±5.0
±0.4 ±2.0 ±0.6 ±3.0
±0.3 ±15 ±0.25 ±12.5
±0.3 ±3.0 ±0.5 ±5.0
±0.3 ±30 ±0.3 ±30
Technical data
Combitips advanced
English (EN)
®
17
18
Technical data
Combitips advanced
®
English (EN)
Test conditions and test evaluation in compliance with ISO 8655, Part 6. Analytical balance with
evaporation protection inspected by the office of weights and measures..
• Number of determinations: 10
• Use of water in accordance with ISO 3696
• Inspection at 20 °C – 25 °C ±0.5 °C
• Dispensing on the inner wall of the tube
The test volumes for the systematic and random errors of the Multipette M4/Repeater M4
comply with the requirements of ISO 8655, part 5.
6.3 Errors with the Multipette plus/Repeater plus
Combitip advanced Volume range Error limits
Error
Systematic error Random error
± %± µL ± %± µL
Technical data
Combitips advanced
English (EN)
®
19
0.1 mL
white
0.2 mL
light blue
0.5 mL
purple
1 mL
yellow
2.5 mL
green
5 mL
blue
10 mL
orange
25 mL
red
2 μL ±1.6 ±0.032 ±3.0 ±0.06
20 μL ±1.0 ±0.2 ±2.0 ±0.4
4 μL ±1.3 ±0.052 ±2.0 ±0.08
40 μL ±0.8 ±0.32 ±1.5 ±0.6
10 μL ±0.9 ±0.09 ±1.5 ±0.15
100 μL ±0.8 ±0.8 ±0.6 ±0.6
20 μL ±0.9 ±0.18 ±0.9 ±0.18
200 μL ±0.6 ±1.2 ±0.4 ±0.8
50 μL ±0.8 ±0.4 ±0.8 ±0.4
500 μL ±0.5 ±2.5 ±0.3 ±1.5
100 μL ±0.6 ±0.6 ±0.6 ±0.6
1 000 μL ±0.5 ±5.0 ±0.25 ±2.5
200 μL ±0.5 ±1.0 ±0.6 ±1.2
2 000 μL ±0.5 ±10 ±0.25 ±5.0
500 μL ±0.4 ±2.0 ±0.6 ±3.0
5 000 μL ±0.3 ±15 ±0.25 ±12.5
50 mL
light gray
Test conditions and test evaluation in compliance with ISO 8655, Part 6. Analytical balance with
evaporation protection inspected by the office of weights and measures.
• Number of determinations: 10
• Use of water in accordance with ISO 3696
• Inspection at 20 °C – 25 °C ±0.5 °C
• Dispensing on the inner wall of the tube
The test volumes for the systematic and random errors of the Multipette (X)stream/Repeater
(X)stream comply with the requirements of ISO 8655, part 5.
1 000 μL ±0.3 ±3.0 ±0.5 ±5.0
10 000 μL ±0.3 ±30 ±0.3 ±30
20
Technical data
Combitips advanced
English (EN)
®
6.4 Errors with the Multipette (X)stream/Repeater (X)stream
Combitip advanced Volume range Testing
volume
Systematic error Random error
± %± µL ± %± µL
0.1 mL
white
Increment:
0.1 μL
0.2 mL
light blue
Increment:
0.2 μL
0.5 mL
purple
Increment:
0.5 μL
1 mL
yellow
Increment:
1 μL
2.5 mL
green
Increment:
2.5 μL
5 mL
blue
Increment:
5 μL
mL mL mL ± %± mL ± %± mL
10 mL
orange
Increment:
0.01 mL
25 mL
red
Increment:
0.025 mL
50 mL
light gray
Increment:
0.05 mL
1 μL – 100 μL 10 μL ±1.6 ±0.16 ±2.5 ±0.25
50 μL ±1.0 ±0.5 ±1.5 ±0.75
100 μL ±1.0 ±1.0 ±0.5 ±0.5
2 μL – 200 μL 20 μL ±1.3 ±0.26 ±1.5 ±0.3
100 μL ±1.0 ±1.0 ±1.0 ±1.0
200 μL ±1.0 ±2.0 ±0.5 ±1.0
5 μL – 500 μL 50 μL ±0.9 ±0.45 ±0.8 ±0.4
250 μL ±0.9 ±2.25 ±0.5 ±1.25
500 μL ±0.9 ±4.5 ±0.3 ±1.5
10 μL – 1 000 μL 100 μL ±0.9 ±0.9 ±0.55 ±0.55
500 μL ±0.6 ±3.0 ±0.3 ±1.5
1 000 μL ±0.6 ±6.0 ±0.2 ±2.00
25 μL – 2 500 μL 250 μL ±0.8 ±2.0 ±0.45 ±1.125
1 250 μL ±0.5 ±6.25 ±0.3 ±3.75
2 500 μL ±0.5 ±12.5 ±0.15 ±3.75
50 μL – 5 000 μL 500 μL ±0.8 ±4.0 ±0.35 ±1.75
2 500 μL ±0.5 ±12.5 ±0.25 ±6.25
5 000 μL ±0.5 ±25 ±0.15 ±7.50
0.1 mL –
0.25 mL – 25 mL 2.5 mL ±0.3 ±0.0075 ±0.35 ±0.0088
0.5 mL – 50 mL 5 mL ±0.3 ±0.015 ±0.5 ±0.025
10 mL
1 mL ±0.5 ±0.005 ±0.25 ±0.0025
5 mL ±0.4 ±0.02 ±0.25 ±0.0125
10 mL ±0.4 ±0.04 ±0.15 ±0.015
12.5 mL ±0.3 ±0.0375 ±0.25 ±0.0313
25 mL ±0.3 ±0.075 ±0.15 ±0.0375
25 mL ±0.3 ±0.075 ±0.20 ±0.05
50 mL ±0.3 ±0.15 ±0.15 ±0.075
Error limits
Error
Technical data
Combitips advanced
English (EN)
Test conditions and test analysis in accordance with ISO 8655, part 6. Test with an analytical balance with a
moisture trap which has been inspected by the Office of Weights and Measures.
• Number of determinations: 10
• Use of water in accordance with ISO 3696
• Inspection at 20 °C – 25 °C ± 0.5 °C
• Dispensing onto the tube wall
• Volume tests in the mode Dis
• Speed levels set: 7
The test volumes for the systematic and random error of the Multipette (X)stream/Repeater
(X)stream comply with the requirements of ISO 8655, part 5.
®
21
22
Technical data
Combitips advanced
English (EN)
®
6.5 Adjustable dispensing volumes
Applies to following dispensers:
• Multipette M4/Repeater M4
• Multipette plus/Repeater plus
Selection
dial
● 100 1.0 μL 2.0 μL 5.0 μL 10 μL 25 μL 50 μL 0.1 mL 0.25 mL 0.5 mL
1 50 2.0 μL 4.0 μL 10 μL 20 μL 50 μL 100 μL 0.2 mL 0.50 mL 1.0 mL
● 33 3.0 μL 6.0 μL 15 μL 30 μL 75 μL 150 μL 0.3 mL 0.75 mL 1.5 mL
2 25 4.0 μL 8.0 μL 20 μL 40 μL 100 μL 200 μL 0.4 mL 1.00 mL 2.0 mL
● 20 5.0 μL 10 μL 25 μL 50 μL 125 μL 250 μL 0.5 mL 1.25 mL 2.5 mL
3 16 6.0 μL 12 μL 30 μL 60 μL 150 μL 300 μL 0.6 mL 1.50 mL 3.0 mL
● 14 7.0 μL 14 μL 35 μL 70 μL 175 μL 350 μL 0.7 mL 1.75 mL 3.5 mL
4 12 8.0 μL 16 μL 40 μL 80 μL 200 μL 400 μL 0.8 mL 2.00 mL 4.0 mL
● 11 9.0 μL 18 μL 45 μL 90 μL 225 μL 450 μL 0.9 mL
5 10 10 μL 20 μL 50 μL 100 μL 250 μL 500 μL 1.0 mL 2.50 mL 5.0 mL
●9 11 μL 22 μL 55 μL 110 μL 275 μL 550 μL 1.1 mL 2.75 mL 5.5 mL
68 12 μL 24 μL 60 μL 120 μL 300 μL 600 μL 1.2 mL 3.00 mL 6.0 mL
●7 13 μL 26 μL 65 μL 130 μL 325 μL 650 μL 1.3 mL 3.25 mL 6.5 mL
Dispensing steps
0.1 mL 0.2 mL 0.5 mL 1.0 mL 2.5 mL 5.0 mL 10 mL 25 mL 50 mL
white light
blue
purple yellow green blue orange red light
gray
2.25 mL 4.5 mL
77 14 μL 28 μL 70 μL 140 μL 350 μL 700 μL 1.4 mL 3.50 mL 7.0 mL
●6 15 μL 30 μL 75 μL 150 μL 375 μL 750 μL 1.5 mL 3.75 mL 7.5 mL
86 16 μL 32 μL 80 μL 160 μL 400 μL 800 μL 1.6 mL 4.00 mL 8.0 mL
●5 17 μL 34 μL 85 μL 170 μL 425 μL 850 μL 1.7 mL 4.25 mL 8.5 mL
95 18 μL 36 μL 90 μL 180 μL 450 μL 900 μL 1.8 mL 4.50 mL 9.0 mL
●5 19 μL 38 μL 95 μL 190 μL 475 μL 950 μL 1.9 mL 4.75 mL 9.5 mL
10
5 20 μL 40 μL 100 μL 200 μL 500 μL 1000 μL 2.0 mL 5.00 mL 10.0 mL
7 Purity grades and certificates
7.1 Eppendorf purity grades
Purity grades and certificates
Combitips advanced
English (EN)
®
23
Eppendorf
Quality
Human DNA-free ■ ■
DNA-free (Human + Bacteria
DNA-free)
DNase-free ■ ■
RNase-free ■ ■
PCR inhibitor-free ■ ■
ATP-free ■
Pyrogen-free (Endotoxin-free) ■ ■
Sterile (Ph.Eur./USP) ■ ■
Sterile PCR clean Biopur
7.2 Certificates
■
Various certificates for Eppendorf consumables can be downloaded from our website www.eppendorf.de/
consumables.
• Batch-specific certificates
Batch-specific certificates are available for Eppendorf consumables with Sterile, PCR clean and Biopur
degrees of purity. These certificates are produced by an independent, recognized laboratory. The batch
number can be found on the label of the folding box.
• General quality certificates
• ISO certificate
24
Ordering Information
Combitips advanced
English (EN)
®
8 Ordering Information
8.1 Combitips advanced
Order no.
(International)
0030 089.405
–
0030 089.618
0030 089.766
0030 089.413
–
0030 089.626
0030 089.774
0030 089.421
–
0030 089.634
0030 089.782
0030 089.430
–
0030 089.642
0030 089.790
0030 089.448
–
0030 089.650
0030 089.804
0030 089.456
–
0030 089.669
0030 089.812
Order no. (North
America)
0030089405
0030089510
0030089618
–
0030089413
0030089529
0030089626
–
0030089421
0030089537
0030089634
–
0030089430
0030089545
0030089642
–
0030089448
0030089553
0030089650
–
0030089456
0030089561
0030089669
–
Description
Combitips advanced 0.1 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 0.2 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 0.5 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 1.0 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 2.5 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 5.0 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Ordering Information
Combitips advanced
English (EN)
®
25
Order no.
(International)
0030 089.464
–
0030 089.677
0030 089.820
0030 089.472
–
0030 089.685
0030 089.839
0030 089.480
–
0030 089.693
0030 089.847
Order no. (North
America)
0030089464
0030089570
0030089677
–
0030089472
0030089588
0030089685
–
0030089480
0030089596
0030089693
–
Description
Combitips advanced 10 mL
100 pieces
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 25 mL
100 pieces + 4 Adapter
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Combitips advanced 50 mL
100 pieces + 4 Adapter
Eppendorf Quality
Sterile, individually wrapped
Biopur, individually wrapped
PCR clean
Order no.
(International)
Order no. (North
America)
0030 089.715 0030089715
0030 089.723 0030089723
0030 089.731 0030089731
0030 089.740 0030089740
Description
Adapter advanced 25 mL
1 piece
Eppendorf Quality
Adapter advanced 50 mL
1 piece
Eppendorf Quality
Adapter advanced 25 mL
7 pieces
Biopur, individually wrapped
Adapter advanced 50 mL
7 pieces
Biopur, individually wrapped
26
Ordering Information
Combitips advanced
®
English (EN)
8.2 Accessories
Combitips ad
®
anced
v
Combilong/Combitube
The Combilong/Combitube is an aspiration tool for
the Combitips advanced. It enables liquids to be
directly taken out of all bottles.
Order no.
(International)
Order no. (North
America)
Description
Combilong
Aspirating aid for removing liquids from volumetric flasks and
tall bottles
0030 059.506 –
2 pieces
Combitube
Aspirating aid for removing liquids from volumetric flasks and
tall bottles
– 022261550
2 pieces
Combitips advanced Rack
1 piece
0030 089.758 0030089758
Eppendorf Quality
Combitips advanced Rack
A Combitips advanced Rack is available for storing
the Combitips advanced (≤ 10 mL).
8.2.1 Multipette M4/Repeater M4
Ordering Information
Combitips advanced
English (EN)
®
27
Order no.
(International)
4982 000.012 – Multipette M4
– 4982000020 Repeater M4
4982 000.314 –
– 4982000322
Order no. (North
America)
Description
Multipette M4 Starter Kit
Multipette M4, Combitip Rack, Combitip Assortmentpack
Repeater M4 Starter Kit
Repeater M4, Combitip Rack, Combitip Assortmentpack
8.3 Multipette plus/Repeater plus
Order no.
(International)
4981 000.019 – Multipette plus
– 022260201 Repeater plus
Order no. (North
America)
Description
8.4 Multipette (X)stream/Repeater (X)stream
Order no.
(International)
4986 000.017 – Multipette stream
4986 000.025 – Multipette Xstream
– 022460803 Repeater stream
– 022460811 Repeater Xstream
Order no. (North
America)
Description
28
Transport, storage and disposal
Combitips advanced
English (EN)
®
9 Transport, storage and disposal
9.1 Storage
NOTICE! Damage due to UV radiation
Do not store consumables in areas with strong UV radiation.
Air temperature Relative humidity Atmospheric pressure
In transport packaging -25 °C – 45 °C 10 % – 95 %70 kPa – 106 kPa
Without transport
packaging
-5 °C – 45 °C 10 % – 95 %70 kPa – 106 kPa
Evaluate your manual
Give us your feedback.
www.eppendorf.com/manualfeedback
Your local distributor: www.eppendorf.com/worldwide
Eppendorf AG · Hamburg · Germany · Phone: +49 40 538 01-0
www.eppendorf.com
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