Bio-Rad GS-900 Calibrated Densitometer User Manual

QC Colloidal Coomassie Stain

161-0803

QC Colloidal Coomassie Stain, 1 L

Gel Size

Fixing Solution*,

QC Colloidal

Water Washes,

Ordering Information

Catalog # Product

Introduction

Bio-Rad’s QC colloidal Coomassie stain is a ready-to-use single-bottle protein stain that does not require the
mixing of any components or addition of any alcohols. It is a special formulation of Coomassie G-250 that
provides maximum sensitivity with low background for a wide variety of acrylamide gel chemistries. The QC
colloidal Coomassie stain can reliably detect BSA in amounts down to 3 ng.

The QC colloidal Coomassie stain does not contain any methanol or acetic acid, which must be disposed of as
hazardous waste.

Kit Contents

Kit contains 1 L of QC colloidal Coomassie stain, which is sufficient to stain 10 Criterion™ gels or 20
Mini-PROTEAN® gels.

Storage Conditions

The QC colloidal Coomassie stain should be stored and used at room temperature. Do not freeze or refrigerate
the stain.

User-Supplied Materials

• Deionized water

• Shallow tray with cover for gel staining and destaining

• Ethanol and acetic acid, if gel fixation is desired

Staining Protocol

This protocol provides the maximal sensitivity while maintaining low background levels and provides the most
consistent and robust results. This protocol allows detection of amounts down to 3 ng of BSA.

ml

Criterion 100 100 100
Mini-PROTEAN 50 50 50

* 40% ethanol, 10% acetic acid

Coomassie Stain, ml

ml

Gel Fixation

Fixation is recommended for maximum sensitivity and staining of low molecular weight proteins <20 kDa.

• Prepare fixing solution (40% ethanol, 10% acetic acid)

• Remove gel from cassette and rinse in a shallow staining tray with deionized water