Zeiss Standard 25 ICS User Manual
Standard 25
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Transmitted Light
Microscope
Operating Manual
Standard 25
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Knowledge of this manual is required for the operation of the instrument. Would you ther efore please
make yourself familiar with the contents of t his manual and pay special att ention to hint s concerning the
safe operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, forw arding and duplic ation of t his document , and the utiliz ation and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by:
Carl Zeiss
Mikroskopie
D-07740 Jena
Telephone: (**49) 03641 / 64-1616
Telefax: (**49) 03641 / 64-3144
Internet: mikro@zeiss.de
http://www.zeiss.de
Number of this manual: B 40-029 e
Date of issue: 01/98
II B 40-029 e 01/98
Standard 25
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CONTENTS
Page
INTRODUCTION........................................................................................................................I
Title page...................................................................................................................................I
Copyright.................................................................................................................................. II
Contents...................................................................................................................................III
Notes on instrument safety.......................................................................................................IV
Warranty notes ........................................................................................................................VI
Overall view ............................................................................................................................VII
1 DESCRIPTION........................................................................................................................1-3
1.1 Name and intended application ..............................................................................................1-3
1.2 Instrument description and main features................................................................................1-3
1.3 Microscope configurations and modules .................................................................................1-4
1.4 Objectives ..............................................................................................................................1-6
1.5 Eyepieces ...............................................................................................................................1-7
1.6 Stage micrometers and eyepiece reticles .................................................................................1-8
1.7 Technical Data......................................................................................................................1-10
2 START-UP .............................................................................................................................2-3
2.1 Unpacking the instrument......................................................................................................2-3
2.2 Attachment of binocular tube 45°/20 ICS or binocular phototube 35°/20 ICS...........................2-3
2.3 Screwing-in of objectives........................................................................................................2-3
2. Insertion of eyepieces.............................................................................................................2-4
2.4.1 Insertion of eyepiece reticle.....................................................................................................2-4
2.4.2 Compensation of ametropia when eyepiece reticles are used ..................................................2-5
2.5 Setting of interpupillary distance and viewing height...............................................................2-5
2.6 Attachment of specimen holder to mechanical stage 75x30 R.................................................2-6
2.7 Attachment of condensers......................................................................................................2-7
2.7.1 Attach condenser 0.9 Z ..........................................................................................................2-7
2.7.2 Attach condenser II Z 0.9 Ph1, 2, 3 and D (0.6/0.9) .................................................................2-7
2.8 Connecting the instrument to the line.....................................................................................2-8
B 40-029 e 01/98 III
Standard 25
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3 OPERATION..........................................................................................................................3-3
3.1 Switch on the instrument.......................................................................................................3-3
3.2 Setting of transmitted-light brightfield for KÖHLER illumination...............................................3-4
3.3 Setting of transmitted-light phase contrast............................................................................. 3-6
3.4 Setting of transmitted-light darkfield......................................................................................3-8
3.5 Setting of transmitted light polarization contrast..................................................................... 3-9
3.6 Measurement of lengths...................................................................................................... 3-10
3.7 Attachment of photomicrography equipment....................................................................... 3-11
3.7.1 Attachment of SLR camera, e.g. CONTAX 167 MT................................................................ 3-11
3.7.2 Attachment of MC 80 DX microscope camera (35 mm film cassette)..................................... 3-13
3.8 Attachment of adapters for video cameras............................................................................3-14
3.9 Insertion of 8x drawing eyepiece.......................................................................................... 3-16
4 CARE, MAINTENANCE AND TROUBLESHOOTING............................................................... 4-3
4.1 Care and maintenance of the instrument................................................................................4-3
4.2 Troubleshooting..................................................................................................................... 4-4
4.2.1 Changing the fuses................................................................................................................4-4
4.2.2 Changing the 6 V 20 W halogen lamp.................................................................................... 4-5
4.2.3 Troubleshooting table ...........................................................................................................4-6
4.3 Table of spares, wearing parts and tools .............................................................................. 4-10
4.4 Requesting service................................................................................................................4-11
Annex...................................................................................................................................A-1
List of abbreviations ...............................................................................................................A-3
Physical and technical units.....................................................................................................A-4
Certification in accordance with DIN EN ISO 9001 / DIN EN 46001...........................................A-5
EC conformity declaration......................................................................................................A-7
IV B 40-029 e 01/98
Standard 25
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Notes on instrument safety
The Standard 25
microscope was designed, produced and tested in compliance with DIN 61010-1
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(IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and
meets the requirements of appendix I of directive 73/ 23/EC and the relevant CSA and UL directives. The
microscope meets the requirements of the EC direct ive 89/336/EC and the EMC legislation of November
9, 1992. This operation manual includes information and warnings which must be observed by the user.
The following warning and information symbols are used in this manual:
NOTE
This symbol (hand) is a warning which you must observe under all circumstances.
CAUTION
This symbol (warning triangle) is a warning which indicates a hazar d to the operation of the
instrument
.
CAUTION
Disconnect the instrument from the line!
B 40-029 e 01/98 V
Standard 25
ICS
The Standard 25
microscope, including its original accessories, may only be used for the microscope
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techniques described in this manual.
Particular attention must be paid to the following warning notes:
The Standard 25
microscope is categorized as Protection Class I and has been allocated the
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protection degree IP 20. The power plug must be inserted in a properly installed socket
featuring a grounding contact. The grounding effect must not be made ineffective by an
extension cable which does not have a protective ground wire.
Before switching on the instrument, check whether it is suitable for the line voltage present.
Admissible voltage: 100...240 V AC, 50...60 Hz (depending on the model).
Always disconnect the instrument from the line before opening the instrument, before
changing the voltage and before changing the fuses.
When changing the instrument fuses, make sure to use only those of the rated power required
and the type indicated. The use of makeshift fuses and the short-circuiting of the fuse holders
are not permitted.
If it is established that the protection measures are no longer ef fective, t he instrument must be
switched off and safeguarded against inadvertent operation.
Dust and dirt can impair the performance of the instrument. Therefore, protect the instrument
against these influences as far as possible. If the microscope will not be used for longer
periods of time, it must be protected using the dust cover.
The instrument must be operated by trained personnel only who must be aware of the
possible danger involved with microscopy and the relevant application.
VI B 40-029 e 01/98
Standard 25
ICS
The Standard 25
performance or damaged when handled improperly.
Notes on warranty:
The manufacturer guarantees that the instrument has no material and production defects
when delivered. You must inform us of any defects immediately and we must do anything to
minimize the damage. If the manufacturer is informed of such a defect, he is obliged to
remove it; it is his decision whether he does this by re pairing the instr ument or by deliver ing an
instrument free of any defect. No guarantee is provided for defects caused by natural wear
(wearing parts in particular).
microscope is an optical precision instrument which can be impair ed in its
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The instrument manufacturer is not liable for damage caused by f aulty operation, negligence
or any other meddling with the instrument, or the use of accessories from other
manufacturers. This forfeits all the claims against warranty.
With the exception of the work specified in this manual, no maintenance or repair of the
Standard 25
may be performed. Repairs may only be performed by Zeiss service staff or
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specially authorized personnel. Should any defect occur with the instrument, please get in
touch with your local Zeiss agency first.
B 40-029 e 01/98 VII
Standard 25
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Overall view of the Standard 25
microscope with polarization equipment
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VIII B 40-029 e 01/98
Standard 25
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DESCRIPTION
Contents
1 DESCRIPTION.................................................................................................................1-3
1.1 Name and intended application........................................................................................1-3
1.2 Instrument description and main features.........................................................................1-3
1.3 Microscope configurations and modules...........................................................................1-4
1.4 Objectives........................................................................................................................1-6
1.5 Eyepieces.........................................................................................................................1-8
1.6 Stage micrometers and eyepiece reticles...........................................................................1-8
1.7 Technical Data...............................................................................................................1-10
List of illustrations
Fig. 1-1 Microscope modules........................................................................................................1-2
Fig. 1-2 Standard 25
microscope configurations.......................................................................1-4
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B 40-029 e 01/98 1-1
Standard 25
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Fig. 1-1 Microscope modules
1 Eyepieces 5 Condenser carrier with condenser
2 Binocular tube 6 Mechanical stage with specimen holder
3 Stand 7 Objective
4 Coaxial coarse and fine drive 8 Objective nosepiece
1-2 B 40-029 e 01/98
Standard 25
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1 DESCRIPTION
1.1 Name and intended application
Manufacturer's name:Standard 25 ICS microscope
The Standard 25
forms in biology and medicine. These are typical application fields of the Standard 25
laboratory microscopy
doctor's offices
training (schools and universities).
microscope is a universal microscope for the visualization of fine structures and
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:
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1.2 Instrument description and main features
Thanks to its pyramid design, the Standard 25
is a compact transmitted-light microscope. In addition
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to high-resolution ICS objectives and the major brightfield, darkfield, phase contrast and polarization
contrast techniques, an optional camera port for photo and video documentation is also available to the
user.
Major instrument features:
Sturdy and convenient stand in the pyramid design.
User-friendly coaxial coarse and fine drive
Mechanical stage 75x30 R with ceramic-coated stage surface and specimen holder
Space-saving and continuously adjustable, integrated illuminator with long-life 6V 20W halogen lamp.
Swing-in 0.9 Z condenser for brightfield, and Ph condenser II Z 0.9 for br ightfield and phase contrast
Ph1, 2 and 3.
Ball-bearing, 5-position nosepiece with W 0.8" thread.
ICS objectives in the price/performance categories CP-Achromat, A-Plan and Achroplan.
Binocular tube 45°/20 ICS with a viewing height of approx. 440 mm, and binocular photot ube 35°/ 20
with sliding prism 100 obs /100 doc.
10x eyepieces for the field numbers 20 or 18, suitable for spectacle wearers, fixed and adjustable.
B 40-029 e 01/98 1-3
1.3 Microscope configurations and modules
Standard 25
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Fig. 1-2 Standard 25
microscope configurations with polarization equipment
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1-4 B 40-029 e 01/98
Standard 25
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Cat. No.
Configurations
Standard 25
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binocular microscope for transmitted-light brightfield and
phase contrast using CP Achromat objectives 10x, 40x Ph2 and 100x
"Standard 25
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" binocular microscope for transmitted-light brightfield
using objectives "A-Plan" 10x, 40x and 100x
"Standard 25
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" binocular microscope for transmitted-light brightfield and
phase contrast using objectives "A-Plan" 10x, 40x Ph 2 and 100x
"Standard 25
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" binocular microscope for transmitted-light brightfield and
phase contrast using objectives "A-Plan" 10x, 20x Ph 2 and 40x Ph 2
"Standard 25
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" binocular microscope for transmitted-light brightfield and
phase contrast using objectives "A-Plan" 10x Ph 1, 40x Ph 2 and 100x Ph 3
"Standard 25
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" binocular microscope for transmitted-light brightfield using
objectives "A-Plan" 10x, 40x and 100x and phototube
"Standard 25
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" binocular microscope for transmitted-light brightfield and phase contrast
using objectives "A-Plan" 10x, 40x Ph 2 and 100x and phototube
"Standard 25
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" binocular microscope for transmitted-light brightfield and phase contrast
using objectives "A-Plan" 10x Ph 1, 40x Ph 2 and 100x Ph 3 and phototube
490841 9804
490841 9904
490845 9804
490845 9904
490846 9804
490846 9904
490847 9804
490847 9904
490848 9804
490848 9904
490865 9804
490865 9904
490866 9804
490866 9904
490868 9804
490868 9904
Modules
1.1 "Standard 25
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" microscope stand with mechanical stage 75x30 R,
5-position nosepiece W 0.8, including integrated illuminator
stabilized power supply 220 ... 240 V/50 ... 60 Hz / 45 VA 450815 9902
1.1.1 Line cable with European plug
1.2 "Standard 25
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" microscope stand with mechanical stage 75x30 R,
5-position nosepiece W 0,8, including integrated illuminator
stabilized power supply 100 ... 127 V / 50 ... 60 Hz / 45 VA 450816 9902
1.2.1 Line cable with American flat plug
1.3 Long-life 6 V 20 W halogen lamp (spare lamp) 380079 9690
1.4 Conversion filter CB 3; d=32x2 mm 467852
1.5 Conversion filter CB 12, d=32x2 mm 467850 9901
1.6 Interference wide-band filter, green, d=32x4 mm 467803
2.1 Binocular tube 45°/20
2.2 Binocular phototube 35°/20
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ICS (
100% obs / 50% obs : 50% doc) 452929
452928
3.1 Swing-in 0.9 Z condenser 445211
3.2 Phase stop carrier Ph 2 for 445211 470864
3.3 Phase contrast condenser II Z 0.9 Ph 1, 2, 3 and D (0.6/0.9) 445210
not shown Dry darkfield condenser 0.7 - 0.85 and Z condenser holder 465506, 445215
not shown Dry darkfield condenser 0.8 - 0.95 and Z condenser holder 465505, 445215
not shown Ultra-condenser 1.2 - 1.4 and Z condenser holder 465500, 445215
3.4 Diopter d=30 mm 444020
4.1 Dust cover K 459300
4.2 Dust cover G (if binocular phototube is used) 459306
All the above microscope configurations with catalogue numbers ending in 9804 feature the line voltage 230 V,
and those ending in 9904 have a line voltage of 115 V.
B 40-029 e 01/98 1-5
Standard 25
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1.4 Objectives
The objectives are the optical centerpiece of the microscope. The following is an example of how
objectives can be labelled:
CP ACHROMAT 10x/0.25 /where
10x = objective magnification, with a defined color ring on the objective being allocated to each
magnification step (Zeiss color code)
0.25 = numerical aperture
= infinite tube length
- : can be used with cover slip thickness D = 0 or 0.17
or
0.17 = can be used with cover slip thickness D = 0.17
and
Oil = oil immersion objective
Ph 2 = phase contrast objective with a green color ring and phase stop Ph 2
The objective magnification multiplied by the eyepiece magnification (minimum 10x) results in the visual
overall magnification, e.g. 10 x 10 = 100x.
The numerical aperture x 1000, e.g. 0.25x1000 = 250x, is the highest useful magnification, i.e. no
further details are resolved above that limit.
The objective labeling indicates that these objectives may only be used with microscopes
featuring an infinite tube length and not with instrument s the objectives of which are marked
with "160" as their mechanical tube length.
The exact observance of the cover slip thickness of 0.17 mm is all the more necessary the higher the
numeric aperture of the objective. Therefore, so-called "Corr" objectives can be set for different cover
slip thicknesses via a correction ring. For this, a specimen area is searched, and the position of the
correction ring where optimum focus and image contrast are obtained is determined (refocusing is
always required).
Immersion objectives are always insensitive to differences in cover slip thickness.
When immersion objectives are used, the air between the cover slip and the objective is replaced with a
liquid, which is immersion oil in most cases. The plast ic oiler containing 20 ml of 581 N immersion oil is
particularly suitable for this purpose.
Due to their short working distance, objectives 25x and higher feature resilient mounts (specimen
protection). To prevent oil contamination of the specimen when the nosepiece is turned, the resilient
mounts of the immersion objectives can be locked in their lifted position by turning them to the r ight (do
not forget to unlock them again!).
1-6 B 40-029 e 01/98
Standard 25
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The following objectives are available for the Standard 25
Microscopy
Technique
Transm.-light brightfield CP-Achromat 5x/0.12 11.2 - 440920
Phase contrast CP-Achromat 10x/0.25 Ph 1 5.1 - 440931
Transm-light brightfield A-Plan 5x/0.12 9.9 - 441020
Phase contrast A-Plan 10x/0.25 Ph 1 4.4 - 441031
Transm.-light brightfield Achrostigmat 20x/0.45 1.6 0.17 440140
Objective Magnification/Num.
Aperture
CP-Achromat
CP-Achromat 10x/0.25 5.1 - 440930
CP-Achromat 40x/0.65 0.3 0.17 440950
CP-Achromat 100x/1.25 Oil 0.07 0.17 440980
CP-Achromat 40x/0.65 Ph 2 0.3 0.17 440951
CP-Achromat 100x/1.25 Oil Ph 2 0.07 0.17 000000 1007 159
CP-Achromat 100x/1.25 Oil Ph 3 0.07 0.17 440981
A-Plan
A-Plan 10x/0.25 4.4 - 441030
A-Plan 20x/0.45 0.53 0.17 441040
A-Plan 40x/0.65 0.43 0.17 441050
A-Plan 100x/1.25 Oil 0.22 0.17 441080
A-Plan 20x/0.45 Ph 2 0.53 0.17 441041
A-Plan 40x/0.65 Ph 2 0.43 0.17 441051
A-Plan 100x/1.25 Oil Ph 3 0.22 0.17 441081
Achrostigmat
Achrostigmat 40x/0.85 Oil 0.28 0.17 440250
Free working
distance in mm
microscope:
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Cover slip
thickness D in
mm
Cat.No.
Phase contrast Achrostigmat 20x/0.45 Ph 2 1.6 0.17 440141
LD-Achrostigmat 20x/0.30 Ph 1 1.0...2.2 0...2.0 440147
LD-Achrostigmat 32x/0.40 Ph 1 1.5...2.2 0.5...1.5 440149
Achroplan
Transm.-light brightfield Achroplan 4x/0.10 11.1 - 440020
Achroplan 10x/0.25 4.8 - 440030
Achroplan 20x/0.45 2.07 0.17 440040
Achroplan 40x/0.65 0.59 0.17 440050
Achroplan 50x/0.90 Oil 0.29 0.17 440057
Achroplan 63x/0.80 0.29 0.17 440060
Achroplan 63x/0.95 0.15 0
(no cover slip)
Achroplan 100x/1.25 Oil 0.19 0.17 440080
Achroplan 100x/1.25 Oil Iris 0.19 0.17 440086
Phase contrast Achroplan 10x/0.25 Ph 1 4.8 - 440031
Achroplan 20x/0.45 Ph 2 2.07 0.17 440041
Achroplan 40x/0.65 Ph 2 0.59 0.17 440051
Achroplan 100x/1.25 Oil Ph 3 0.19 0.17 440081
Plan-Neofluar
Transm.-light brightfield Plan-Neofluar 2.5x/0.075 9.3 - 440310
440068
B 40-029 e 01/98 1-7
1.5 Eyepieces
The following eyepieces are offered for the Standard 25 ICS:
Eyepiece Image Angle Cat. No.
Eyepiece PL 10x/18 Br. 39° 444131 9901
Eyepiece PL 10x/18 Br. foc. 39° 444132 9901
Eyepiece E-PL 10x/20 Br. 43° 444231 9901
Eyepiece E-PL 10x/20 Br. foc. 43° 444232 9902
Eyepiece PL 16x/16 Br. 54° 444053
Eyepiece PL 16x/16 Br. foc. 54° 444054
If required, eyecups for the eyepieces can be ordered under the Cat. No. 444801.
Standard 25
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1.6 Stage micrometers and eyepiece reticles
Measuring and counting using the microscope requires stage micrometers and eyepiece reticles, a sm all
selection of which is listed below:
Illustration Description, Technical Data Cat. No.
Stage micrometer, positive 5 + 100/100 y D
474026
= 0.17 mm
gradation on the + y-axis: 5 mm in 5 intervals;
gradation on the - y-axis: 1 mm in 100/100 mm
= 10 m, accuracy 1m
Crossline micrometer disk 14:140 / d =26
454060
mm
gradation length=14 mm
increments = 0.1mm
gradation tolerance 0.001 mm
1-8 B 40-029 e 01/98
Standard 25
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Crossline disk / d = 26 mm 474064
Crossline micrometer disk 10:100 / d =26
mm
gradation length=10 mm
increments = 0.1mm
gradation tolerance 0.001 mm
Net micrometer 12.5x12.5/5;10 / d = 26 mm
area 12.5x12.5 mm, divided in fields of 5x5 or
10x10.
Photo reticle MC 2.5x / d = 26 mm
for 35 mm photography with an additional
magnification of 2.5x or for large-format
photography with a 10x additional
magnification.
474066 9901
474068
454075
If an eyepiece reticle is used, the binocular tube or the photot ube must be equipped with two
foc. eyepieces containing an adjustable eyelens, into one of which the eyepiece reticle is
mounted.
B 40-029 e 01/98 1-9
Standard 25
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1.7 Technical Data
(1) Dimensions (width x depth x height)
Stand with binocular tube..........................................................................approx. 200 x 350 x 450 mm
Stand with phototube ...............................................................................approx. 200 x 375 x 460 mm
Overall height including T2 adapter and CONTAX 167 MT camera housing..................... approx. 570 mm
(2) Weight
Standard 25
with binocular tube.................................................................................. approx. 6.7 kg
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(3) Ambient conditions
Storage and transport (in packaging):
Permissible ambient temperature......................................................................................-40 to + 70° C
Permissible relative humidity (without condensation).............................................................max. 100 %
Operation:
Permissible ambient temperature..................................................................................... +10 to + 35° C
Permissible relative humidity (without condensation)...............................................................max. 85 %
Altitude.............................................................................................................................. max. 2000 m
Atmospheric pressure............................................................................................. 800 hPa to 1060 hPa
(4) Operating data
Operation........................................................................................................................... closed rooms
Protection class.......................................................................................................................................I
Enclosure protection........................................................................................................................IP 20
Electrical safety.................................................................in compliance with DIN EN 61010 (IEC 1010-1)
...............................................................................................................including CSA and UL directives
Pollution degree................................................................................................................................... 2
Overvoltage category.............................................................................................................................II
Radio interference suppression.......................................................in accordance with EN 55011, Class B
Line voltage...................................................................................................... 100 to 127 V AC (10%)
or 220 to 240 V AC (10%)
Line frequency...................................................................................................................... 50 to 60 Hz
Label of voltage range...............................................................................................see instrument rear
Power consumption...............................................................................................................max. 45 VA
Output voltage................................................................................stabilized, adjustable from 1.5 to 6 V
1-10 B 40-029 e 01/98
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