Zeiss Standard 25 ICS User Manual

5 (1)

Standard 25 ICS

Transmitted Light

Microscope

Operating Manual

Standard 25 ICS

Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument.

The specifications are subject to change; the manual is not covered by an update service.

©Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted. Violations will entail an obligation to pay compensation.

All rights reserved in the event of granting of patents or registration of a utility model.

Issued by:

Carl Zeiss

 

 

 

Mikroskopie

 

 

D-07740 Jena

 

 

Telephone: (**49) 03641

/ 64-1616

 

Telefax:

(**49) 03641

/ 64-3144

 

Internet:

mikro@zeiss.de

 

 

http://www.zeiss.de

Number of this manual: B 40-029 e

Date of issue: 01/98

II

B 40-029 e 01/98

Standard 25 ICS

 

CONTENTS

 

 

 

Page

 

INTRODUCTION........................................................................................................................

I

 

Title page ...................................................................................................................................

I

 

Copyright..................................................................................................................................

II

 

Contents ...................................................................................................................................

III

 

Notes on instrument safety .......................................................................................................

IV

 

Warranty notes ........................................................................................................................

VI

 

Overall view ............................................................................................................................

VII

1

DESCRIPTION........................................................................................................................

1-3

1.1

Name and intended application ..............................................................................................

1-3

1.2

Instrument description and main features................................................................................

1-3

1.3

Microscope configurations and modules .................................................................................

1-4

1.4

Objectives ..............................................................................................................................

1-6

1.5

Eyepieces ...............................................................................................................................

1-7

1.6

Stage micrometers and eyepiece reticles .................................................................................

1-8

1.7

Technical Data......................................................................................................................

1-10

2

START-UP .............................................................................................................................

2-3

2.1

Unpacking the instrument ......................................................................................................

2-3

2.2

Attachment of binocular tube 45°/20 ICS or binocular phototube 35°/20 ICS...........................

2-3

2.3

Screwing-in of objectives ........................................................................................................

2-3

2.

Insertion of eyepieces .............................................................................................................

2-4

2.4.1 Insertion of eyepiece reticle.....................................................................................................

2-4

2.4.2

Compensation of ametropia when eyepiece reticles are used ..................................................

2-5

2.5

Setting of interpupillary distance and viewing height ...............................................................

2-5

2.6

Attachment of specimen holder to mechanical stage 75x30 R .................................................

2-6

2.7

Attachment of condensers......................................................................................................

2-7

2.7.1 Attach condenser 0.9 Z ..........................................................................................................

2-7

2.7.2

Attach condenser II Z 0.9 Ph1, 2, 3 and D (0.6/0.9) .................................................................

2-7

2.8

Connecting the instrument to the line.....................................................................................

2-8

B 40-029 e 01/98

III

Standard 25 ICS

3

OPERATION..........................................................................................................................

3-3

3.1

Switch on the instrument .......................................................................................................

3-3

3.2

Setting of transmitted-light brightfield for KÖHLER illumination...............................................

3-4

3.3

Setting of transmitted-light phase contrast .............................................................................

3-6

3.4

Setting of transmitted-light darkfield ......................................................................................

3-8

3.5

Setting of transmitted light polarization contrast.....................................................................

3-9

3.6

Measurement of lengths ......................................................................................................

3-10

3.7

Attachment of photomicrography equipment .......................................................................

3-11

3.7.1

Attachment of SLR camera, e.g. CONTAX 167 MT ................................................................

3-11

3.7.2

Attachment of MC 80 DX microscope camera (35 mm film cassette).....................................

3-13

3.8

Attachment of adapters for video cameras............................................................................

3-14

3.9

Insertion of 8x drawing eyepiece ..........................................................................................

3-16

4

CARE, MAINTENANCE AND TROUBLESHOOTING

............................................................... 4-3

4.1

Care and maintenance of the instrument................................................................................

4-3

4.2

Troubleshooting.....................................................................................................................

4-4

4.2.1

Changing the fuses ................................................................................................................

4-4

4.2.2

Changing the 6 V 20 W halogen lamp....................................................................................

4-5

4.2.3

Troubleshooting table ...........................................................................................................

4-6

4.3

Table of spares, wearing parts and tools ..............................................................................

4-10

4.4

Requesting service................................................................................................................

4-11

Annex...................................................................................................................................

A-1

List of abbreviations ...............................................................................................................

A-3

Physical and technical units.....................................................................................................

A-4

Certification in accordance with DIN EN ISO 9001 / DIN EN 46001...........................................

A-5

EC conformity declaration ......................................................................................................

A-7

IV

B 40-029 e 01/98

Standard 25 ICS

Notes on instrument safety

The Standard 25 ICS microscope was designed, produced and tested in compliance with DIN 61010-1 (IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and meets the requirements of appendix I of directive 73/23/EC and the relevant CSA and UL directives. The microscope meets the requirements of the EC directive 89/336/EC and the EMC legislation of November 9, 1992. This operation manual includes information and warnings which must be observed by the user.

The following warning and information symbols are used in this manual:

NOTE

This symbol (hand) is a warning which you must observe under all circumstances.

CAUTION

This symbol (warning triangle) is a warning which indicates a hazard to the operation of the instrument.

CAUTION

Disconnect the instrument from the line!

B 40-029 e 01/98

V

Standard 25 ICS

The Standard 25 ICS microscope, including its original accessories, may only be used for the microscope techniques described in this manual.

Particular attention must be paid to the following warning notes:

The Standard 25 ICS microscope is categorized as Protection Class I and has been allocated the protection degree IP 20. The power plug must be inserted in a properly installed socket featuring a grounding contact. The grounding effect must not be made ineffective by an extension cable which does not have a protective ground wire.

Before switching on the instrument, check whether it is suitable for the line voltage present. Admissible voltage: 100...240 V AC, 50...60 Hz (depending on the model).

Always disconnect the instrument from the line before opening the instrument, before changing the voltage and before changing the fuses.

When changing the instrument fuses, make sure to use only those of the rated power required and the type indicated. The use of makeshift fuses and the short-circuiting of the fuse holders are not permitted.

If it is established that the protection measures are no longer effective, the instrument must be switched off and safeguarded against inadvertent operation.

Dust and dirt can impair the performance of the instrument. Therefore, protect the instrument against these influences as far as possible. If the microscope will not be used for longer periods of time, it must be protected using the dust cover.

The instrument must be operated by trained personnel only who must be aware of the possible danger involved with microscopy and the relevant application.

VI

B 40-029 e 01/98

Standard 25 ICS

The Standard 25 ICS microscope is an optical precision instrument which can be impaired in its performance or damaged when handled improperly.

Notes on warranty:

The manufacturer guarantees that the instrument has no material and production defects when delivered. You must inform us of any defects immediately and we must do anything to minimize the damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision whether he does this by repairing the instrument or by delivering an instrument free of any defect. No guarantee is provided for defects caused by natural wear (wearing parts in particular).

The instrument manufacturer is not liable for damage caused by faulty operation, negligence or any other meddling with the instrument, or the use of accessories from other manufacturers. This forfeits all the claims against warranty.

With the exception of the work specified in this manual, no maintenance or repair of the Standard 25 ICS may be performed. Repairs may only be performed by Zeiss service staff or specially authorized personnel. Should any defect occur with the instrument, please get in touch with your local Zeiss agency first.

B 40-029 e 01/98

VII

Standard 25 ICS

Overall view of the Standard 25 ICS microscope with polarization equipment

VIII

B 40-029 e 01/98

Standard 25 ICS

DESCRIPTION

Contents

1

DESCRIPTION .................................................................................................................

1-3

1.1

Name and intended application........................................................................................

1-3

1.2

Instrument description and main features.........................................................................

1-3

1.3

Microscope configurations and modules...........................................................................

1-4

1.4

Objectives........................................................................................................................

1-6

1.5

Eyepieces.........................................................................................................................

1-8

1.6

Stage micrometers and eyepiece reticles...........................................................................

1-8

1.7

Technical Data ...............................................................................................................

1-10

List of illustrations

 

Fig. 1-1

Microscope modules........................................................................................................

1-2

Fig. 1-2

Standard 25 ICS microscope configurations.......................................................................

1-4

B 40-029 e 01/98

1-1

Standard 25 ICS

Fig. 1-1

Microscope modules

 

 

1

Eyepieces

 

5

Condenser carrier with condenser

2

Binocular tube

6

Mechanical stage with specimen holder

3

Stand

 

7

Objective

4

Coaxial coarse and fine drive

8

Objective nosepiece

1-2

B 40-029 e 01/98

Standard 25 ICS

1 DESCRIPTION

1.1Name and intended application

Manufacturer's name: Standard 25 ICS microscope

The Standard 25 ICS microscope is a universal microscope for the visualization of fine structures and forms in biology and medicine. These are typical application fields of the Standard 25 ICS:

laboratory microscopy

doctor's offices

training (schools and universities).

1.2Instrument description and main features

Thanks to its pyramid design, the Standard 25 ICS is a compact transmitted-light microscope. In addition to high-resolution ICS objectives and the major brightfield, darkfield, phase contrast and polarization contrast techniques, an optional camera port for photo and video documentation is also available to the user.

Major instrument features:

Sturdy and convenient stand in the pyramid design.

User-friendly coaxial coarse and fine drive

Mechanical stage 75x30 R with ceramic-coated stage surface and specimen holder

Space-saving and continuously adjustable, integrated illuminator with long-life 6V 20W halogen lamp.

Swing-in 0.9 Z condenser for brightfield, and Ph condenser II Z 0.9 for brightfield and phase contrast Ph1, 2 and 3.

Ball-bearing, 5-position nosepiece with W 0.8" thread.

ICS objectives in the price/performance categories CP-Achromat, A-Plan and Achroplan.

Binocular tube 45°/20 ICS with a viewing height of approx. 440 mm, and binocular phototube 35°/20 with sliding prism 100 obs /100 doc.

10x eyepieces for the field numbers 20 or 18, suitable for spectacle wearers, fixed and adjustable.

B 40-029 e 01/98

1-3

Zeiss Standard 25 ICS User Manual

Standard 25 ICS

1.3Microscope configurations and modules

Fig. 1-2 Standard 25 ICS microscope configurations with polarization equipment

1-4

B 40-029 e 01/98

Standard 25 ICS

 

Cat. No.

 

 

Configurations

 

Standard 25 ICS binocular microscope for transmitted-light brightfield and

490841 9804

phase contrast using CP Achromat objectives 10x, 40x Ph2 and 100x

490841 9904

"Standard 25 ICS" binocular microscope for transmitted-light brightfield

490845 9804

using objectives "A-Plan" 10x, 40x and 100x

490845 9904

"Standard 25 ICS" binocular microscope for transmitted-light brightfield and

490846 9804

phase contrast using objectives "A-Plan" 10x, 40x Ph 2 and 100x

490846 9904

"Standard 25 ICS" binocular microscope for transmitted-light brightfield and

490847 9804

phase contrast using objectives "A-Plan" 10x, 20x Ph 2 and 40x Ph 2

490847 9904

"Standard 25 ICS" binocular microscope for transmitted-light brightfield and

490848 9804

phase contrast using objectives "A-Plan" 10x Ph 1, 40x Ph 2 and 100x Ph 3

490848 9904

"Standard 25 ICS" binocular microscope for transmitted-light brightfield using

490865 9804

objectives "A-Plan" 10x, 40x and 100x and phototube

490865 9904

"Standard 25 ICS" binocular microscope for transmitted-light brightfield and phase contrast

490866 9804

using objectives "A-Plan" 10x, 40x Ph 2 and 100x and phototube

490866 9904

"Standard 25 ICS" binocular microscope for transmitted-light brightfield and phase contrast

490868 9804

using objectives "A-Plan" 10x Ph 1, 40x Ph 2 and 100x Ph 3 and phototube

490868 9904

Modules

 

1.1"Standard 25 ICS" microscope stand with mechanical stage 75x30 R, 5-position nosepiece W 0.8, including integrated illuminator

stabilized power supply 220 ... 240 V/50 ... 60 Hz / 45 VA

450815 9902

1.1.1Line cable with European plug

1.2"Standard 25 ICS" microscope stand with mechanical stage 75x30 R, 5-position nosepiece W 0,8, including integrated illuminator

stabilized power supply 100 ... 127 V / 50 ... 60 Hz / 45 VA

450816 9902

1.2.1Line cable with American flat plug

1.3

Long-life 6 V 20 W halogen lamp (spare lamp)

380079 9690

1.4

Conversion filter CB 3; d=32x2 mm

467852

1.5

Conversion filter CB 12, d=32x2 mm

467850 9901

1.6

Interference wide-band filter, green, d=32x4 mm

467803

2.1

Binocular tube 45°/20 ICS

452928

2.2

Binocular phototube 35°/20 ICS (100% obs / 50% obs : 50% doc)

452929

3.1

Swing-in 0.9 Z condenser

445211

3.2

Phase stop carrier Ph 2 for 445211

470864

3.3

Phase contrast condenser II Z 0.9 Ph 1, 2, 3 and D (0.6/0.9)

445210

not shown

Dry darkfield condenser 0.7 - 0.85 and Z condenser holder

465506, 445215

not shown

Dry darkfield condenser 0.8 - 0.95 and Z condenser holder

465505, 445215

not shown

Ultra-condenser 1.2 - 1.4 and Z condenser holder

465500, 445215

3.4

Diopter d=30 mm

444020

4.1

Dust cover K

459300

4.2

Dust cover G (if binocular phototube is used)

459306

All the above microscope configurations with catalogue numbers ending in 9804 feature the line voltage 230 V, and those ending in 9904 have a line voltage of 115 V.

B 40-029 e 01/98

1-5

Standard 25 ICS

1.4Objectives

The objectives are the optical centerpiece of the microscope. The following is an example of how objectives can be labelled:

CP ACHROMAT 10x/0.25 /-

where

 

 

10x

=

objective magnification, with a defined color ring on the objective being allocated to each

 

 

magnification step (Zeiss color code)

0.25= numerical aperture

= infinite tube length

-

:

can be used with cover slip thickness D = 0 or 0.17

or

 

 

0.17

=

can be used with cover slip thickness D = 0.17

and

 

 

Oil

=

oil immersion objective

Ph 2

=

phase contrast objective with a green color ring and phase stop Ph 2

The objective magnification multiplied by the eyepiece magnification (minimum 10x) results in the visual overall magnification, e.g. 10 x 10 = 100x.

The numerical aperture x 1000, e.g. 0.25x1000 = 250x, is the highest useful magnification, i.e. no further details are resolved above that limit.

The objective labeling indicates that these objectives may only be used with microscopes featuring an infinite tube length and not with instruments the objectives of which are marked with "160" as their mechanical tube length.

The exact observance of the cover slip thickness of 0.17 mm is all the more necessary the higher the numeric aperture of the objective. Therefore, so-called "Corr" objectives can be set for different cover slip thicknesses via a correction ring. For this, a specimen area is searched, and the position of the correction ring where optimum focus and image contrast are obtained is determined (refocusing is always required).

Immersion objectives are always insensitive to differences in cover slip thickness.

When immersion objectives are used, the air between the cover slip and the objective is replaced with a liquid, which is immersion oil in most cases. The plastic oiler containing 20 ml of 581 N immersion oil is particularly suitable for this purpose.

Due to their short working distance, objectives 25x and higher feature resilient mounts (specimen protection). To prevent oil contamination of the specimen when the nosepiece is turned, the resilient mounts of the immersion objectives can be locked in their lifted position by turning them to the right (do not forget to unlock them again!).

1-6

B 40-029 e 01/98

Standard 25 ICS

The following objectives are available for the Standard 25 ICS microscope:

Microscopy

Objective

Magnification/Num.

Free working

Cover slip

Cat.No.

Technique

 

Aperture

distance in mm

thickness D in

 

 

 

 

 

mm

 

 

CP-Achromat

 

 

 

 

Transm.-light brightfield

CP-Achromat

5x/0.12

11.2

-

440920

 

CP-Achromat

10x/0.25

5.1

-

440930

 

CP-Achromat

40x/0.65

0.3

0.17

440950

 

CP-Achromat

100x/1.25 Oil

0.07

0.17

440980

Phase contrast

CP-Achromat

10x/0.25 Ph 1

5.1

-

440931

 

CP-Achromat

40x/0.65 Ph 2

0.3

0.17

440951

 

CP-Achromat

100x/1.25 Oil Ph 2

0.07

0.17

000000 1007 159

 

CP-Achromat

100x/1.25 Oil Ph 3

0.07

0.17

440981

 

A-Plan

 

 

 

 

Transm-light brightfield

A-Plan

5x/0.12

9.9

-

441020

 

A-Plan

10x/0.25

4.4

-

441030

 

A-Plan

20x/0.45

0.53

0.17

441040

 

A-Plan

40x/0.65

0.43

0.17

441050

 

A-Plan

100x/1.25 Oil

0.22

0.17

441080

Phase contrast

A-Plan

10x/0.25 Ph 1

4.4

-

441031

 

A-Plan

20x/0.45 Ph 2

0.53

0.17

441041

 

A-Plan

40x/0.65 Ph 2

0.43

0.17

441051

 

A-Plan

100x/1.25 Oil Ph 3

0.22

0.17

441081

 

Achrostigmat

 

 

 

 

Transm.-light brightfield

Achrostigmat

20x/0.45

1.6

0.17

440140

 

Achrostigmat

40x/0.85 Oil

0.28

0.17

440250

Phase contrast

Achrostigmat

20x/0.45 Ph 2

1.6

0.17

440141

 

LD-Achrostigmat

20x/0.30 Ph 1

1.0...2.2

0...2.0

440147

 

LD-Achrostigmat

32x/0.40 Ph 1

1.5...2.2

0.5...1.5

440149

 

Achroplan

 

 

 

 

Transm.-light brightfield

Achroplan

4x/0.10

11.1

-

440020

 

Achroplan

10x/0.25

4.8

-

440030

 

Achroplan

20x/0.45

2.07

0.17

440040

 

Achroplan

40x/0.65

0.59

0.17

440050

 

Achroplan

50x/0.90 Oil

0.29

0.17

440057

 

Achroplan

63x/0.80

0.29

0.17

440060

 

Achroplan

63x/0.95

0.15

0

440068

 

 

 

 

(no cover slip)

 

 

Achroplan

100x/1.25 Oil

0.19

0.17

440080

 

Achroplan

100x/1.25 Oil Iris

0.19

0.17

440086

Phase contrast

Achroplan

10x/0.25 Ph 1

4.8

-

440031

 

Achroplan

20x/0.45 Ph 2

2.07

0.17

440041

 

Achroplan

40x/0.65 Ph 2

0.59

0.17

440051

 

Achroplan

100x/1.25 Oil Ph 3

0.19

0.17

440081

 

Plan-Neofluar

 

 

 

 

Transm.-light brightfield

Plan-Neofluar

2.5x/0.075

9.3

-

440310

 

 

 

 

 

 

B 40-029 e 01/98

1-7

Standard 25 ICS

1.5Eyepieces

The following eyepieces are offered for the Standard 25 ICS:

Eyepiece

Image Angle

Cat. No.

 

 

 

Eyepiece PL 10x/18 Br.

39°

444131 9901

 

 

 

Eyepiece PL 10x/18 Br. foc.

39°

444132 9901

 

 

 

Eyepiece E-PL 10x/20 Br.

43°

444231 9901

 

 

 

Eyepiece E-PL 10x/20 Br. foc.

43°

444232 9902

 

 

 

Eyepiece PL 16x/16 Br.

54°

444053

 

 

 

Eyepiece PL 16x/16 Br. foc.

54°

444054

 

 

 

If required, eyecups for the eyepieces can be ordered under the Cat. No. 444801.

1.6Stage micrometers and eyepiece reticles

Measuring and counting using the microscope requires stage micrometers and eyepiece reticles, a small selection of which is listed below:

Illustration

 

Description, Technical Data

Cat. No.

 

 

 

 

 

 

 

 

 

 

 

 

Stage micrometer, positive 5 + 100/100 y D

474026

 

 

 

 

 

= 0.17 mm

 

 

 

 

 

 

gradation on the + y-axis: 5 mm in 5 intervals;

 

 

 

 

 

 

gradation on the - y-axis: 1 mm in 100/100 mm

 

 

 

 

 

 

= 10 m, accuracy 1 m

 

Crossline micrometer disk 14:140 / d =26

454060

mm

 

gradation length=14 mm

 

increments = 0.1mm

 

gradation tolerance 0.001 mm

 

1-8

B 40-029 e 01/98

Standard 25 ICS

Crossline disk / d = 26 mm

474064

 

 

 

 

 

 

 

Crossline micrometer disk 10:100 / d =26

474066 9901

 

 

 

 

 

 

 

 

 

 

 

 

mm

 

 

 

 

 

 

 

 

 

 

 

 

 

 

gradation length=10 mm

 

 

 

 

 

 

 

 

 

 

 

 

 

 

increments = 0.1mm

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

gradation tolerance 0.001 mm

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Net micrometer 12.5x12.5/5;10 / d = 26 mm 474068 area 12.5x12.5 mm, divided in fields of 5x5 or

10x10.

Photo reticle MC 2.5x / d = 26 mm

454075

for 35 mm photography with an additional

magnification

of 2.5x

or

for large-format

photography

with

a

10x

additional

magnification.

 

 

 

 

If an eyepiece reticle is used, the binocular tube or the phototube must be equipped with two foc. eyepieces containing an adjustable eyelens, into one of which the eyepiece reticle is mounted.

B 40-029 e 01/98

1-9

Standard 25 ICS

1.7Technical Data

(1)

Dimensions (width x depth x height)

 

 

Stand with binocular tube..........................................................................

approx.

200 x 350 x 450 mm

Stand with phototube ...............................................................................

approx.

200 x 375 x 460 mm

Overall height including T2 adapter and CONTAX 167 MT camera housing.....................

 

approx. 570 mm

(2)Weight

Standard 25 ICS with binocular tube..................................................................................

approx. 6.7 kg

(3)

Ambient conditions

 

Storage and transport (in packaging):

 

Permissible ambient temperature ......................................................................................

-40 to + 70° C

Permissible relative humidity (without condensation) .............................................................

max. 100 %

Operation:

 

Permissible ambient temperature .....................................................................................

+10 to + 35° C

Permissible relative humidity (without condensation) ...............................................................

max. 85 %

Altitude..............................................................................................................................

max. 2000 m

Atmospheric pressure .............................................................................................

800 hPa to 1060 hPa

(4)

Operating data

 

Operation...........................................................................................................................

closed rooms

Protection class.......................................................................................................................................

I

Enclosure protection........................................................................................................................

IP 20

Electrical safety.................................................................

in compliance with DIN EN 61010 (IEC 1010-1)

 

 

including CSA and UL directives

Pollution degree ...................................................................................................................................

2

Overvoltage category.............................................................................................................................

II

Radio interference suppression.......................................................

in accordance with EN 55011, Class B

Line voltage......................................................................................................

100 to 127 V AC ( 10%)

 

 

or 220 to 240 V AC ( 10%)

Line frequency......................................................................................................................

50 to 60 Hz

Label of voltage range ...............................................................................................

see instrument rear

Power consumption...............................................................................................................

max. 45 VA

Output voltage ................................................................................

stabilized, adjustable from 1.5 to 6 V

1-10

B 40-029 e 01/98

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