Zeiss Primo Star User Manual [en, de, fr]

Primo Star

Operating manual Bedienungsanleitung Mode d’emploi

Instrucciones de manejo

Инструкция по применению

Carl Zeiss	Copyright	Primo Star

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Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning

safe operation			of the instrument. The specifications are subject		to	change; the manual is not covered
by	an	update	service. Unless expressly	authorized, forwarding		and duplication of this document,
as	well	as utilization and communication		of its contents are	not	permitted. Violations will entail an

obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model.

Die Kenntnis dieser Anleitung ist für die Bedienung des Gerätes erforderlich. Bitte machen Sie sich deshalb mit dem Inhalt vertraut und befolgen Sie besonders Hinweise, die den sicheren Umgang mit dem Gerät betreffen. Änderungen im Interesse der technischen Weiterentwicklung bleiben vorbehalten; das Handbuch unterliegt nicht dem Änderungsdienst. Weitergabe sowie Vervielfältigung dieser Unterlage, Verwertung und Mitteilung ihres Inhalts sind nicht gestattet, soweit nicht ausdrücklich zugestanden. Zuwiderhandlungen verpflichten zu Schadenersatz. Alle Rechte für den Fall der Patenterteilung oder Gebrauchsmuster-Eintragung vorbehalten.

L’utilisation de l'appareil suppose la bonne connaissance du présent mode d’emploi. Nous vous prions par conséquent de lire attentivement les informations contenues dans ce document et de respecter notamment les consignes relatives à la sécurité d’utilisation. Le fabricant se réserve le droit d’apporter des modifications techniques en fonction de l’évolution des technologies. Ces modifications ne sont pas automatiquement prises en compte dans le mode d'emploi qui accompagne chaque appareil. Toute divulgation, reproduction ou publication du présent document, même partielle, est interdite sans notre autorisation écrite. Toute infraction donne droit au versement de dommages et intérêts. Tous les droits sont réservés en cas de délivrance d’un brevet ou de dépôt d’un modèle d'utilité.

El manejo de este equipo presupone el conocimiento de las presentes instrucciones. Por eso le rogamos familiarizarse con su contenido y observar en particular las indicaciones que se refieren al manejo seguro del mismo. Nos reservamos el derecho a modificaciones en interés del desarrollo técnico; el manual no está sujeto al servicio de actualización. Sin nuestro consentimiento expreso no se autoriza ni la entrega y reproducción de este manual, ni el aprovechamiento y la comunicación de su contenido. Cualquier contravención implica el pago de una indemnización. Reservados todos los derechos para el otorgamiento de patentes o el registro de modelos de utilidad.

Знание данной инструкции необходимо для использования прибора. Поэтому необходимо ознакомиться с ее содержанием и особенно следовать указаниям, касающихся безопасного обращения с прибором. Фирма оставляет за собой право на изменения в интересах технического усовершенствования; руководство не подлежит изменениям. Передача и тиражирование данных документов, использование и сообщение их содержания не допускаются без особого разрешения. В случае нарушений полагается возмещение убытков. Фирма оставляет за собой право на выдачу патента или регистрацию зарегистрированной модели.

偿责任。保留所有申请专利或者样品注册权利。

Issued by: Carl Zeiss MicroImaging GmbH

P.O.B. 4041, 37030 Göttingen, Germany

Phone:	[phone]
Fax:	[phone]
E-Mail:	[e-mail]

www.zeiss.de

Number of this manual: B 46-0071 v

Date of issue: Version 7, 03/01/2006

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Primo Star	Contents	Carl Zeiss

CONTENTS
		Page
1	Notes on Instrument Safety..............................................................................................	4
1.1	General safety notes.............................................................................................................	4
1.2	Instrument safety and EMC ..................................................................................................	4
1.3	Unpacking, transportation, storage.......................................................................................	5
1.4	Disposal................................................................................................................................	5
1.5	Use.......................................................................................................................................	5
1.6	Notes on warranty................................................................................................................	7
2	Description .........................................................................................................................	8
2.1	System overview...................................................................................................................	8
2.2	Total view...........................................................................................................................	10
2.3	Intended use ......................................................................................................................	11
2.4	Instrument description and main features ...........................................................................	11
2.5	Objectives...........................................................................................................................	12
3	Start-Up and Operation ..................................................................................................	13
3.1	Starting up the microscope.................................................................................................	13
3.2	Controls .............................................................................................................................	14
3.3	Operating the microscope ..................................................................................................	16
3.3.1	Setting interpupillary distance and viewing height ..............................................................	16
3.3.2	Compensating for ametropia and inserting the eyepiece pointer.........................................	16
3.3.3	Adjusting transmitted-light brightfield on the Full Köhler microscope..................................	17
3.3.4	Adjusting transmitted-light brightfield on the Fixed Köhler microscope ...............................	18
3.3.5	Adjusting transmitted-light phase contrast or transmitted-light darkfield.............................	19
3.4	Converting the microscope.................................................................................................	20
3.4.1	Changing the tube .............................................................................................................	20
3.4.2	Inserting color filters ...........................................................................................................	20
3.4.3	Replacing the 6V 30W halogen lamp or the LED illumination..............................................	21
3.4.4	Changing objectives ...........................................................................................................	21
3.4.5	Installing/removing the illuminating mirror..........................................................................	22
3.4.6	Installing a camera..............................................................................................................	23
4	Care and Troubleshooting ..............................................................................................	25
4.1	Instrument care ..................................................................................................................	25
4.2	Troubleshooting .................................................................................................................	26
5	Appendix..........................................................................................................................	27
5.1	Technical data ....................................................................................................................	27

English

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English

Carl Zeiss Notes on Instrument Safety Primo Star

1 NOTES ON INSTRUMENT SAFETY

1.1General safety notes

Please read this Operating Manual carefully before starting up the microscope.

If you need supplementary information, contact Carl Zeiss Service or an authorized agency.

To ensure safe operation and troublefree function of the microscope, strictly observe the precautions and warnings given in this manual.

These are set off herein as follows:

CAUTION

This symbol indicates a possible hazard to the user of the instrument.

CAUTION

This symbol indicates a possible hazard to the instrument or system.

CAUTION

Disconnect the plug-in power unit from line power before opening the microscope!

NOTE

This symbol refers you to advice that you must observe under all circumstances.

1.2Instrument safety and EMC

The Primo Star microscope has been designed, produced and tested in compliance with the standards DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 "Safety requirements for electrical equipment for measurement, control and laboratory use “.

The Primo Star microscope meets the requirements of the EC Directive 98/79/EC Annex 1 and carries the mark.

Radio-noise suppression in compliance with EN 55011 Class B

Noise immunity in compliance with DIN EN 61326

The instruments are disposed of in compliance with the WEEE Directive 2002/96/EC.

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Primo Star	Notes on Instrument Safety	Carl Zeiss

1.3Unpacking, transportation, storage

Please observe the following safety notes for unpacking, transportation and storage of the microscope:

−The microscope is supplied packed to commercial standards in a plastic case with cardboard packaging; use the original packaging only for any transportation.

−Retain the original packaging for longer storage or return to the manufacturer.

−When unpacking the equipment, verify that all parts specified on the delivery note are present.

− Keep transport and storage temperatures as specified in Technical Data.

−Set up the microscope on a stable worktable with solid and smooth tabletop.

−Do not touch optical surfaces.

English

1.4Disposal

Please observe the following safety notes for the disposal of the microscope:

Defective microscopes should not be disposed of with household waste; dispose of them in compliance with the provisions of the law.

1.5Use

The microscope including its original accessories must not be used for microscopic techniques other than those described in this Operating Manual.

Please observe the following safety notes when using the microscope:

The manufacturer cannot assume any liability for other applications, including those of individual modules or single components. This also applies to any service or repair work that is not carried out by authorized service personnel. In case of non-compliance, all warranty claims shall be forfeited.

The microscope may only be operated by trained personnel who must be aware of the possible dangers involved with microscopy and the particular application concerned. The microscope may only be operated if set up on a stable, solid and smooth surface.

The stereomicroscope is a high-precision instrument that can be impaired in its performance or even be destroyed when handled improperly.

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Carl Zeiss	Notes on Instrument Safety	Primo Star

The microscope is equipped with a plug-in power unit allowing line voltages to be used in the range between 100 and 240 V ± 10%, 50/60 Hz, without the need for changing the voltage setting on the instrument.

The plug-in power unit meets the requirements of Protection Class II (with protective insulation). If its casing is damaged, put the plug-in power unit out of operation. The microscope may be operated only with the supplied plug-in power unit.

If it is determined that protection measures are no longer effective, the instrument must be switched off and safeguarded against inadvertent operation. Please contact a Zeiss service agency or the Carl Zeiss Microscopy Service to repair the instrument.

−Always disconnect the power cable, before opening the instrument and changing the lamp or LED source.

−Wait for the lamp to cool down before replacing it and do not touch the new bulb.

−The instrument may only be opened by qualified specialists or service staff.

−The operation of the instrument in explosion-risk environments is not allowed.

Make sure to read the safety notes provided with Immersol 518 N® immersion oil.

Immersion oil irritates the skin. Avoid any contact with skin, eyes and clothing. After skin contact, wash the oil off with plenty of water and soap.

After eye contact, immediately rinse the eye with plenty of water for at least five minutes. If the irritation persists, consult a specialist.

Proper disposal of immersion oil: Take care to ensure that immersion oil does not enter surface water or the sewage system.

The microscope is not equipped with special devices for the protection from substances that are corrosive, potentially infectious, toxic, radioactive, or other substances that could be hazardous to health. Make sure to observe all legal regulations, particularly the relevant national accident prevention regulations when handling such substances.

−Before any transport of the instrument, switch it off and let it cool down (hot surface at the bottom of the instrument).

−Operate the device only on a hard, non-combustible support.

−The plug-in power unit must not get in contact with moisture.

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Primo Star	Notes on Instrument Safety	Carl Zeiss

1.6Notes on warranty

The Primo Star Microscope including its original accessories must not be used for microscopic techniques other than those described in this Operating Manual. The manufacturer cannot assume any liability for other applications.

Please note the following information on warranty for the Primo Star Microscope:

−The manufacturer guarantees that the microscope is free of material or manufacturing defects when delivered.

−Possible defects must be notified to us immediately and steps be taken to minimize damage.

−If notified of such a defect, the manufacturer is obligated to rectify it at his discretion, either by repairing the instrument or delivering an intact replacement.

−No guarantee is provided for defects caused by natural wear (wearing parts in particular) and improper use.

−The instrument manufacturer shall not be liable for damage caused by faulty operation, negligence or any other tampering with the microscope, particularly the removal or replacement of microscope components, or the use of accessories from other manufacturers.

Unauthorized tampering with the instrument shall lead to a forfeit of all warranty claims.

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	Carl Zeiss	Description	Primo Star
English	2	DESCRIPTION
	2.1	System overview
	8		B 46-0071 v 03/06

Primo Star	Description	Carl Zeiss
		English
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Carl Zeiss	Description	Primo Star

2.2Total view

English

1 Eyepiece

2 Binocular head

3 Tube

4 Carrying handle

5 Plug-in power unit

6 Stand

7 ON/OFF switch; illumination intensity

8 Illumination module

9a Luminous-field diaphragm, adjustable

9b Luminous-field diaphragm, fixed

10a Condenser carrier, Full Köhler

10b Condenser carrier, Fixed Köhler

11a Abbe condenser, Full Köhler

11b Abbe condenser, Fixed Köhler

12 Objective

13 Microscope stage

14 Objective nosepiece

Fig. 1	Total view

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Primo Star	Description	Carl Zeiss

2.3Intended use

The Primo Star microscope is a universally applicable light microscope primarily designed for the examination of cell and tissue cultures as well as sediments in culture flasks, Petri dishes and microplates.

Typical applications:

Examination of blood and tissue samples from the human body, observation of intracellular processes on living cell cultures, cell-cell interactions, motility, growth, potential measurement, detection of medical drugs, microinjection and in vitro fertilization.

When handling hazardous substances, observe the instructions on intended operation, correct use and statutory safety precautions.

English

2.4Instrument description and main features

The Primo Star is a transmitted-light microscope of compact design with a small footprint.

Beside the high-resolution, infinity-corrected objectives and the important microscopy techniques, such as brightfield, darkfield and phase contrast, the microscope is optionally available with a camera port for photo and video documentation.

The major features of the microscope include:

−Modular illumination through 6V 30W halogen lamp, LED illumination or illuminating mirror.

−Integrated fixture for external power supply and cable (incl. cable with multi-standard plug and country-specific plug adapters)

−Plastic-coated carrying handle integrated in stand for installation, uninstallation and transport

−Blue, light-intensity indicators installed on both sides and well visible from a distance

−Stand in "Full Köhler" or "Fixed Köhler" design

−Convenient coaxial coarse and fine focusing drive, smoothness of coarse focusing drive being adjustable

−Mechanical stage 75×30 left/right with specimen holder, stage controls optionally on the right or left

−Space-saving, continuously adjustable illumination module optionally with halogen or LED source

−"Full Köhler" or "Fixed Köhler" Abbe condensers for brightfield, darkfield and Ph 2 phase contrast

−Backward inclined quadruple objective nosepiece with W 0.8 lens thread running on ball bearing

−Infinity-corrected "Plan-ACHROMAT" objectives with magnifications of 4x, 10x, 40x and 100x/Oil for brightfield, darkfield and Ph 2 phase contrast Ph 2 (40x/Ph2) as well as for oil immersion applications (100x/Oil)

−Binocular tube or binocular phototube (50% vis, 50% doc) with ergonomically favorable tube angle of 30° with adjustable interpupillary distance and viewing height

−10× focusing eyepieces for field-of-view numbers 18 or 20, suitable for spectacle wearers

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Carl Zeiss	Description	Primo Star

2.5Objectives

The objectives are the optical heart of the microscope. The objectives may be labeled as follows: Plan-ACHROMAT 10×/0.25 ∞/-.

		where:
		10×	Objective magnification,
			With a defined color ring on the
			objective being assigned to each
			magnification step (Carl Zeiss color code)
		0.25	Numerical aperture
		∞	Infinite mechanical tube length
		−	Usable with cover slip thickness D = 0 or
			0.17 mm
		or
		0.17 Usable with cover slip thickness
			D = 0.17 mm
		Other labels:
		Oil	Oil immersion objective
Fig. 2	Objective	Ph 2 Phase contrast objective with green
			inscription and phase stop Ph 2

Objective magnification multiplied by eyepiece magnification results in overall visual magnification, e.g. 10 x 10 = 100x.

Numerical aperture multiplied by 1000, e.g. 0.25 × 1000 = 250×, presents the maximum useful magnification; there is no resolution for further details above that limit.

When immersion objectives are used, the air between the cover slip and the objective is replaced by a liquid, which in most cases is immersion oil. The plastic oiler containing 5 ml of immersion oil is particularly suitable for this purpose.

Because of their short working distances, the 40×, 40x/Ph 2 and 100x/Oil objectives are equipped with resilient mounts for specimen protection.

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Primo Star	Start-Up and Operation	Carl Zeiss

3	START-UP AND OPERATION

3.1Starting up the microscope

Before installing and starting up the microscope, be sure to carefully read and observe the notes on instrument safety (see Section 1).

Do not touch optical surfaces when unpacking the microscope to avoid fingerprints!

The microscope is supplied completely assembled and inclusive of accessories packed to commercial standards. Additionally ordered components, such as sliders or transmitted-light equipment with illuminating mirror, are packed separately.

• Remove the microscope from the transport case and place it onto the worktable.

Retain the original packaging for storage of the instrument in longer periods of non-use or for return to the manufacturer.

•Remove the plug-in power unit (Fig. 3/2) from its storage fixture on the back wall of the microscope.

•Replace the installed power outlet adapter by

one of the supplied country-specific adapters (Fig. 3/4), if necessary. To this end, pull off the attached adapter and plug on the desired adapter.

• Connect the plug-in power unit to a power outlet.

	• If the plug-in power unit cannot be plugged
	into the chosen power outlet because of limited
	space, replace the power outlet adapter by the
	supplied IEC adapter (Fig. 3/3). This allows the
	plug-in power unit to be put flat onto the
	tabletop and connected to the power outlet	Fig. 3	Starting up the microscope
	through a country-specific appliance cable.

When using the IEC adapter, the plug-in power unit can be fixed to the back wall of the microscope stand by means of the supplied two self-adhesive hooks and the Velcro® strip.

•Turn on the microscope with rotary switch (Fig. 3/5) and adjust the illumination to the desired intensity.

The selected intensity is indicated in five steps by the blue light-emitting diodes (Fig. 3/1) arranged on both sides of the stand.

•At the end of the microscopic examinations, turn the microscope off with the rotary switch and cover it with the dust cover.

•The smoothness of the coarse focusing drive (Fig. 4/18) is factory-adjusted. However, you can readjust it when required.

English

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Carl Zeiss	Start-Up and Operation	Primo Star

3.2	Controls
English
Fig. 4	Controls
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Primo Star

Start-Up and Operation

Legend to Fig. 4:

1Eyepieces

2Binocular body of the tube

3Illumination-intensity indicators

4Rotary knob for switch ON/OFF and adjustment of illumination intensity

5Fine focusing drive (right side)

6Coarse focusing drive (right side)

7Control knob for X travel of mechanical stage

8Control knob for Y travel of mechanical stage

9Condenser clamp screw

10a Knurled ring for adjustment of luminous-field diaphragm (with Full Köhler equipment only) 10b Luminous-field diaphragm (fixed in Fixed Köhler equipment)

11a Condenser entering screws (in Full Köhler equipment: knurled screws) 11b Condenser centering screws (in Fixed Köhler equipment: Allen screws)

12Spring lever of specimen holder

13Knurled ring of objective nosepiece

14Lever for the adjustment of the aperture diaphragm of the condenser

15Knurled knob for vertical adjustment of condenser

16Coarse focusing drive (left side)

17Fine focusing drive (left side)

18Knurled ring for adjusting the smoothness of the coarse focusing drive

Carl Zeiss

English

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Carl Zeiss	Start-Up and Operation	Primo Star

Fig. 5 Setting the interpupillary distance

Fig. 6 Adjusting the viewing height

3.3Operating the microscope

3.3.1Setting interpupillary distance and viewing height

•Swing the eyepiece tubes symmetrically slightly toward or away from one another to adjust the distance between the tubes to your individual interpupillary distance (Fig. 5).

The adjustment of the interpupillary distance is correct when you see only one round image while looking through the two eyepieces!

•Swivel the eyepiece tubes fully up (Fig. 6/A) or down (Fig. 6/B) to adjust the viewing height to your individual requirements.

3.3.2Compensating for ametropia and inserting the eyepiece pointer

The eyepieces (Fig. 7/3) are equipped with foldover rubber eyecups (Fig. 7/1: pulled out; Fig. 7/2: folded over).

Both eyepieces are suitable for spectacle wearers. Additionally, they contain a focusing ring for the compensation of defective vision. The provided diopter scale serves to facilitate finding the correct setting.

The eyepiece pointer can be inserted in one eyepiece, when required.

To this end, follow this procedure:

•Use Allen key SW 1 mm to loosen grub screw (Fig. 7/6) on the binocular body from the bottom; remove the eyepiece.

•Unscrew the stop (Fig. 7/5) by hand from the eyepiece.

•Insert the eyepiece pointer (Fig. 7/4) into the eyepiece (with the coated side facing your eyes). Screw in the eyepiece stop again.

•Put the eyepiece into the tube and fix it with the grub screw.

Fig. 7 Inserting the eyepiece pointer

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Primo Star		Start-Up and Operation	Carl Zeiss
•	Turn the focusing ring of the eyepiece (Fig. 7/3) to focus on the triangular figure of the eyepiece
	pointer.				English
•	eyepiece pointer and focus on the microscopic image by turning on the focusing drive.
	Put the specimen onto the mechanical stage. Look at the specimen through the eyepiece with the
• When in the above-mentioned eyepiece both microscopic image and eyepiece pointer appear sharply,
	focus the image for the second eye by turning the focusing ring of the second eyepiece.

Having done so, both microscopic images inclusive of the eyepiece pointer are focused.

Afterwards, you should focus on the specimen only by adjusting the focusing drive.

3.3.3Adjusting transmitted-light brightfield on the Full Köhler microscope

•First, place a contrasty specimen slide with the 0.17 mm cover slip being on top in the

specimen holder of the mechanical stage. Fix the slide by means of the spring lever (Fig. 4/12).

•If the microscope stand is equipped with a phase or dark-filed slider, pull this slider out to the left as far as it will go.

•Turn rotary knob (Fig. 8/1) on microscope stand to adjust the illumination intensity.

•Move the Abbe condenser up to the top

mechanical stop by turning knurled knob (Fig. 4/15); set the control lever of the aperture diaphragm (Fig. 4/14) to mid-position.

The knurled knob for vertical adjustment of the condenser is at the left of the microscope, if you use the mechanical stage 75x30 with drive on the right, it is at the right, if the stage controls are on the left.

• Turning at the knurled ring (Fig. 8/6) of	the
nosepiece, swing the 10x objective into	the	Fig. 8		Adjusting transmitted-light
light path.				brightfield

•On the binocular tube (Fig. 8/7), first look through one eyepiece and turn the focusing drive (Fig. 8/2) to focus on the specimen.

•Then, readjust the focus for the other eye, if necessary, by turning the eye lens of the focusing eyepiece.

•Close the luminous-field diaphragm (Fig. 8/3) until it becomes visible (even if not in focus) in the field of view (Fig. 8/A).

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Carl Zeiss	Start-Up and Operation	Primo Star

•Turn the knurled knob for vertical adjustment of the condenser (Fig. 4/15) until the edge of the luminous-field diaphragm appears sufficiently sharp (Fig. 8/B).

•Center the image of the luminous-field diaphragm using both centering screws (Fig. 8/5) of the

condenser (Fig. 8/C). Then, open the diaphragm until it just disappears from the field of view (Fig. 8/D).

•To adjust the aperture diaphragm (contrast), remove one eyepiece from the tube and look through the tube with your naked eye. Swing lever (Fig. 8/4) to adjust the aperture diaphragm to approximately 2/3 ... 4/5 of the diameter of the exit pupil of the objective (Fig. 8/E). In most applications, this aperture diaphragm setting provides optimum contrast at almost ideal resolution, and is therefore the best compromise for the human eye.

•Insert the eyepiece back in the tube.

Specimen field size and objective aperture change after every objective change. Therefore, repeat the adjustment of luminous-field diaphragm and aperture diaphragm to obtain optimum results.

3.3.4Adjusting transmitted-light brightfield on the Fixed Köhler microscope

The Primo Star Fixed Köhler Microscope is supplied factory-adjusted. Operation is restricted to a few manipulations.

•Place the specimen in the specimen holder of the mechanical stage.

•If the microscope stand is equipped with a phase or dark-filed slider, pull this slider out to the left as far as it will go.

•Adjust the desired magnification by swinging in the corresponding objective.

•Set the control lever of the aperture diaphragm of the condenser to the value of the selected magnification (10x, 40x or 100x).

•Turn the rotary knob on the microscope stand to adjust the illumination intensity to a level pleasant for observation.

If the condenser had been removed (e.g. for installing the illuminating mirror), make sure to reinstall and center it by means of the two centering screws (for this, refer to Section 3.4.5).

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Primo Star	Start-Up and Operation	Carl Zeiss

3.3.5Adjusting transmitted-light phase contrast or transmitted-light darkfield

•First, adjust the microscope as you do for brightfield.

•Turn the nosepiece to swing the phase-contrast objective (40x/Ph 2) into the light path.

•Open the luminous-field diaphragm (Fig. 9/3, if adjustable) on the stand and open the aperture diaphragm by means of control lever (Fig. 9/1) on Abbe condenser (Fig. 9/2).

•If the phase-contrast slider (Fig. 9/6) was not factory-installed yet, first unscrew its screw (Fig. 9/7). Then, push the slider from the left into the Abbe condenser and screw the screw (Fig. 9/7) back in again.

•Push the slider to the right as far as it will go to move the phase stop into the light path.

•Move the control lever of the aperture diaphragm to fully open it on the Full Köhler model or set it to the PH mark on the Fixed Köhler model.

•Adjust the illumination intensity as needed.

•Check the centering of the phase stop according to the drawing in Fig. 10. To this end, remove one eyepiece and replace it by the eyepiece telescope.

•Center the phase stop, if necessary, (Fig. 10/A) by turning the two adjusting screws of the slider (Fig. 9/5) by means of the two Allen keys SW 1.5 (Fig. 9/4) until the phase stop image corresponds to that shown in Fig. 10/B.

•Afterwards, replace the eyepiece telescope by the eyepiece again.

Fig. 9 Inserting the slider

Fig. 10 Centering the phase stop

For darkfield applications, use the darkfield slider in place of the phase-contrast slider.

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English

Carl Zeiss	Start-Up and Operation	Primo Star

Fig. 11 Changing the tube

3.4Converting the microscope

Unplug the plug-in power unit from line power before converting the microscope.

3.4.1Changing the tube

•Loosen clamp screw (Fig. 11/2), turn the installed tube (Fig. 11/1) clockwise by approximately 90° (Fig. 11/A) and on the right side take it of upward (Fig. 11/B).

For reasons of space, the tube may also be clamped by the grub screw with hexagonal socket head supplied with the tube.

•Insert the tube to be installed, with the eyepieces pointing to the right and its dovetail mount slightly inclined, under the two holding elements (Fig. 11/4) in the stand.

•Then, place the tube horizontally onto the stand. In doing so, the groove on the underside of the tube must be located above the third holding element (Fig. 11/3) of the stand.

•Turn the tube counterclockwise by 45° (eyepieces pointing frontward), align it to the stand and tighten clamp screw (Fig. 11/2).

For space-saving storage of the microscope (e.g. in a cabinet) the tube may also be rotated by 180° to the back.

3.4.2Inserting color filters

• Move the condenser carrier fully up by turning the knurled screw for vertical adjustment (Fig. 4/15).

•Unscrew the cover cap (Fig. 12/3) from the luminous-field diaphragm (Fig. 12/1).

•Put the desired filter – yellow, green or blue – (Fig. 12/2) onto the mounting surface of the luminous-field diaphragm and screw the cover cap back into place.

Fig. 12 Inserting a color filter

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Primo Star	Start-Up and Operation	Carl Zeiss

3.4.3Replacing the 6V 30W halogen lamp or the LED illumination

Unplug the plug-in power unit from the power outlet and allow for a sufficient cool-down time of the 6V 30W halogen lamp before you replace it.

•Loosen both fastening screws (Fig. 13/3) of the illumination module (Fig. 13/1 o 2). In doing so, slightly press the screws against the spring and turn them by 90°: Turn left screw clockwise and right screw counterclockwise.

•Pull the illumination module (Fig. 13/1 or 2) out of the stand.

•If the stand is equipped with a 6V 30W halogen

lamp (Fig. 13/2), remove the halogen lamp (Fig. 13/4) from the lamp carrier and insert the new halogen lamp. Do not touch the new lamp with naked fingers as this will reduce lamp life.

•If the stand is equipped with an LED source, replace the complete illumination module including the LED (Fig. 13/1).

•Push the illumination module (Fig. 13/1 or 2) back into the stand and lock it by means of the two screws (Fig. 13/3). In doing so, slightly press the screws against the spring and turn them by 90°: Turn left screw counterclockwise and right screw clockwise.

3.4.4Changing objectives

•Turn the focusing drive to move the mechanical stage fully down.

•Turn the nosepiece (Fig. 14/3) to move the objective to be changed (Fig. 14/1) into a lateral position.

•Unscrew the objective and remove it downward.

•Screw the desired objective (Fig. 14/2) into the nosepiece as far as it will go.

•If you intend to insert the objective in a lens mount not used so far, remove the dust cover from the corresponding mount of the nosepiece.

English

Fig. 13 Replacing the 6V 30W halogen lamp or the LED illumination module

Fig. 14 Changing an objective

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21

English

Carl Zeiss	Start-Up and Operation	Primo Star

Fig. 15 Removing/installing the condenser

Fig. 16 Installing/removing the illuminating mirror

3.4.5Installing/removing the illuminating mirror

The mirror serves to illuminate the specimen if no power outlet is available.

It can be used only in combination with the Primo Star Fixed Köhler Microscope. For this purpose, it is necessary to remove the condenser and the condenser carrier insert.

Installing the illuminating mirror:

•Remove cover cap (Fig. 15/6) from luminousfield diaphragm (Fig. 15/5).

•Using an Allen key, loosen the clamp screw

(Fig. 15/2) of the condenser and the centering screws (Fig. 15/3) of the condenser carrier (Fig. 15/4) so that the condenser (Fig. 15/7) can be removed frontward. Remove the condenser (Fig. 15/7).

• Unscrew clamp screw (Fig. 15/2 or Fig. 16/1) from condenser carrier insert (Fig. 15/1 or Fig. 16/3).

•Push the condenser carrier backward against the spring and inclining it remove it upward from the condenser carrier (Fig. 15/4).

•Insert the mirror (Fig. 16/5) from top through the aperture of the condenser carrier and place it onto the mount of the luminous-field diaphragm (Fig. 16/2). Take care that the mirror rests level on the mount.

•Rotate and incline the mirror until the daylight is reflected homogeneously into the light path.

Removing the illuminating mirror:

•Remove the mirror (Fig. 16/5) upwards through the aperture of the condenser carrier.

• Slightly incline the condenser carrier	insert
(Fig. 15/1 or Fig. 16/3) to insert it	in the

aperture of the condenser carrier. In doing so, press the unit backward against the spring and place it horizontally onto the condenser carrier.

•Screw the condenser clamp screw (Fig. 16/1) into the condenser carrier insert.

22	B 46-0071 v 03/06

Primo Star	Start-Up and Operation	Carl Zeiss
• Taking care that the locating screw on the rear side of the condenser fits in groove (Fig. 16/4) of the
condenser carrier insert, put the condenser into this insert.				English
• Screw the cover cap back onto the luminous-field diaphragm.
• To center the condenser, connect the microscope to the power outlet and switch it on.
• Turn the nosepiece to swing the 4x objective into the light path.
• Turn the two centering screws (Fig. 15/3) to adjust the illuminated image symmetrically to the edge of
the field of view.

3.4.6Installing a camera

You can install a digital camera, a video camera or a compact digital camera to your choice on the microscope via the available camera adapters (see Section 2.1).

•First, install the photo tube (Fig. 17/7), if necessary (refer to Section 3.4.1).

•Loosen clamp screw (Fig. 17/8) and remove the dust cap from the photo tube.

Installing a compact digital camera

•	Sliding mount (Fig. 17/4), thread adapter ring
	M37/52 (Fig. 17/3) and lens mount (Fig. 17/6)
	are supplied premounted as Digital Camera
	Adapter P95 M37/52x0.75. The opposite
	drawing shows this unit dismantled.
	Furthermore, you may unscrew the M37/52
	thread adapter (Fig. 17/3) (not shown) from the
	sliding mount (Fig. 17/4) so that you can also
	mount cameras with M37 thread.
•	Mount the adapter ring (Fig. 17/2) (supplied	Fig. 17	Installing a camera
	with the camera) to the camera (Fig. 17/1) (see
	the operating instructions of the camera).

•Screw the unit consisting of sliding mount (Fig. 17/4), thread adapter ring M37/52 (Fig. 17/3) and lens mount (Fig. 17/6) into the adapter ring (Fig. 17/2).

•Insert the camera with adapter into the photo tube as far as it will go. Align it and fasten it with clamp screw (Fig. 17/8).

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		Carl Zeiss	Start-Up and Operation	Primo Star
		• Depending on the microscope equipment or the camera used, it may be necessary to optimize the
English		distance between camera lens and lens mount (Fig. 17/6) (see double-headed arrow). This will be
		necessary in particular, if it is impossible to obtain an unvignetted image in any of the zoom positions
		of the camera lens. To this end, adjust the camera as follows:
		− Switch off the autofocus.
		− Set the object distance to ∞.
		− Set aperture-priority auto exposure mode.
		− Choose an aperture as large as possible (i.e. small aperture value!).

Not all the cameras provide these options. Please consult the Operating Manual of the used camera.

•Loosen grub screw (Fig. 17/5).

•Vary the camera lens/lens mount distance gradually, i.e. displace the sliding mount with camera on lens mount in defined steps.

•Zoom the camera lens through from wide angle (W) to tele position (T).

•Carry out this test until the image is format filling without masking or vignetting.

•Retighten the grub screw (Fig. 17/5).

With camera/adapter combinations that have not been recommended expressly by Zeiss, it may be quite impossible to obtain an unvignetted image.

Installing a digital camera or video camera with C-mount thread

Cameras with C-mount thread are to be connected to the phototube of the microscope by means of the camera adapter P95-C 2/3” 0.65x or P95-C ½” 0.5x (Fig. 17/9).

•Put the camera (Fig. 17/10) together with the corresponding camera adapter (Fig. 17/9) into the phototube as far as it will go, align it and fasten it by means of the clamp screw (Fig. 17/8).

24	B 46-0071 v 03/06

Primo Star	Care and Troubleshooting	Carl Zeiss

4	CARE AND TROUBLESHOOTING

4.1Instrument care

Care of the microscope is restricted to the following operations:

•Cover the instrument with the dust cover after every use.

•Do not install the microscope in a humid room; maximum humidity < 75%.

•Cover open tubes with the dust caps.

•Remove dust and loose dirt from visible optical surfaces with a brush, blower brush, cotton bud, optics cleaning tissue, or a cotton cloth.

•Remove water-soluble dirt (coffee, cola, etc.) by blowing on it and subsequent wiping it off with a cloth moistened with water to which you may also add a mild detergent.

•Wipe off stubborn oily or fatty dirt (immersion oil, fingerprints) with a cotton bud or a lint-free cotton cloth and the optics cleaning solution L.

The cleaning solution consists of 90 vol% gasoline and 10 vol% isopropanol (IPA). Its individual constituents are also known as:

Gasoline:	Medical alcohol, petrolether
Isopropanol:	2-Propanol,
	Dimethylcarbinol,
	2-Hydroxypropane

Clean optical surfaces by polishing in circles starting in the middle and moving to the edges using slight pressure only.

For the use in humid climatic zones, all optical components of the microscope are provided with protection against fungus attack.

English

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25

Carl Zeiss	Care and Troubleshooting	Primo Star

4.2Troubleshooting

English
		The field of view is not	Nosepiece with objective has not been		Switch nosepiece with objective into
		Problem	Cause		Remedy
		completely visible.	switched into click-stop position.		click-stop position.
			Condenser has not been set correctly.		Set condenser correctly.
			The aperture diaphragm has not been		Adjust aperture diaphragm correctly.
			adjusted correctly.
			The luminous-field diaphragm has not		Adjust luminous-field diaphragm
			been adjusted correctly.		correctly.
			The filter has not been inserted		Insert filter correctly into filter mount.
			correctly in the filter mount.
		Low resolving power, poor	The aperture diaphragm has not been		Set opening of aperture diaphragm to
		image contrast	opened to the correct size.		correct size.
			Condenser not focused correctly.		Focus the condenser.
			Wrong cover slip thickness selected		Use standard 0.17 mm cover slips.
			for use of transmitted-light objectives
			corrected for 0.17mm cover slips.
			Use of no or non-specified immersion		Use the supplied immersion oil.
			oil with immersion objective
			Air bubbles in immersion oil		Remove the bubbles by applying new oil
					or moving the objective to and fro.
			Immersion oil on the front lens of a		Clean the front lens of the dry objective.
			dry objective.
			Dirt or dust on the optical surfaces of		Clean the respective optical
			objectives, eyepieces, condenser, or		components.
			filters
		Greater focus differences after	The focusing eyepieces have not been		Set the focusing eyepieces to the
		changing the objective	set correctly.		corresponding defective vision.
		The 6V 30W halogen lamp or	Power plug has not been plugged into		Connect power plug to power outlet.
		the LED source does not light	power outlet.
		up although the microscope	6V 30W halogen lamp or LED source is		Replace the defective 6V 30W halogen
		has been switched on.
			defective.		lamp or the LED module
		The 6V 30W halogen lamp is	End of average service life of 6V 30W		Replace the 6V 30W halogen lamp.
		flickering, its light intensity	halogen lamp has been reached.
		unstable, the illumination
			Power cable Incorrectly installed or		Connect the power cable correctly or
		inhomogeneous
			broken power cable		replace it.
			The pins of the 6V 30W halogen lamp		Insert the pins of the 6V 30W halogen
			have not been inserted correctly in the		lamp correctly in the lamp holder.
			lamp holder.
			The pins of the 6V 30W halogen lamp		Insert the pins of the 6V 30W halogen
			have not been inserted symmetrically		lamp symmetrically in the lamp holder.
			in the lamp holder.
		Stage comes down by itself,	Adjusted torque of coarse focusing		Increase the torque of the coarse
		image focus is unstable	drive is too low.		focusing drive to make motion stiffer.

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Primo Star	Appendix	Carl Zeiss

5	APPENDIX
5.1	Technical data
Dimensions (width x depth x height)
Stand with binocular tube		approx. 190 x 425 x 395 mm
Stand with phototube		approx. 190 x 425 x 395 mm
With tube / phototube turned by 180°		approx. 190 x 375 x 395 mm
Weight
Primo Star with phototube		8.2 kg
Ambient conditions
Transport (in packaging):
	Permissible ambient temperature	-40 to +70 °C
Storage:
	Permissible ambient temperature	+10 to +40 °C
	Permissible humidity	max. 75% at 35 °C
	(no condensation)
Operation:
	Permissible ambient temperature	+5 to +40 °C
	Permissible humidity	max. 75% at 35 °C
	(no condensation)
	Atmospheric pressure	800 hPa to 1060 hPa
Operating data
Protection Class		II
Protection Type		IP20
Electrical safety		in compliance with DIN EN 61010-1 (IEC 61010-1)
		including CSA and UL directives
Pollution degree		2
Overvoltage category		II
Radio interference suppression		in accordance with EN 61326
Line voltage		100 to 240 V (±10 %) wide-range input power supply, i.e. the
		voltage setting of the instrument need not be changed!
Line frequency		50 / 60 Hz
Power consumption		70 VA; secondary voltage of external power supply 12V
Output of plug-in power unit		12 V DC; max. 2.5A
Microscope 12V/6V DC		1.5V – 6V adjustable
Light sources
Halogen lamp		HAL 6V 30W
	Adjustability of light source	continuous, from 1.5 to 6V DC
	Color temperature at 6V	2800 K
	Luminous flux	280 lm
	Average service life	1000 h
	Luminous area	1.5 x 3 mm

English

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Carl Zeiss	Appendix	Primo Star

English

LED illumination
Constant, brightness-independent
color temperature of	7480 K
Homogeneous field illumination	20 mm diameter
Suitable for objectives with
magnifications of	4x to 100x
Analogous brightness adjustment from	approx. 15 to 100%

Optical/mechanical data

Stand with stage focusing
With coarse focusing drive	4 mm/revolution
With fine focusing drive	0.5 mm/revolution
Total stage lift	15 mm
Objective change	Manual via quadruple objective nosepiece
Objectives	Infinity-corrected objective range with W 0.8 mounting thread
Eyepieces	30 mm tube size
With field-of-view number 18	PL 10x/18 Br. foc.
With field-of-view number 20	PL 10x/20 Br. foc.
Specimen stage	Mechanical stage 75x30 right/left
Dimensions (width x depth)	140 x 135 mm
Stage travel (X x Y)	75 x 30 mm
Coaxial drive	Optionally right or left
Vernier scales	Readable from the right
Specimen holder	With spring lever, left
Abbe condenser 0.9/1.25, fixed collector	For Vobj. 4x to 100x
Abbe condenser 0.9/1.25	For Vobj. 4x to 100x
Binocular tube 30°/20
Maximum field-of-view number	20
Interpupillary distance	Adjustable from 48 to 75 mm
Tube angle	30°
Viewing height	380 to 415 mm
Viewing port	Tube factor 1x
Binocular phototube 30°/20
Maximum field-of-view number	20
Interpupillary distance	Adjustable from 48 to 75 mm
Tube angle	30°
Viewing height	380 to 415 mm
Viewing port	Tube factor 1x
Photo/video port	Tube factor 1x, 60 mm mount
Light path selection with swing-in prism	50% vis / 100% doc
Illuminating mirror	With plane surface and spherical surface with f' = 75 mm

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Primo Star	Inhaltsübersicht	Carl Zeiss

INHALTSÜBERSICHT
		Seite
1	Hinweise zur Gerätesicherheit .........................................................................................	4
1.1	Allgemeine Sicherheitshinweise ............................................................................................	4
1.2	Gerätesicherheit und EMV ....................................................................................................	4
1.3	Auspacken, Transportieren, Lagern.......................................................................................	5
1.4	Entsorgen.............................................................................................................................	5
1.5	Bedienen ..............................................................................................................................	5
1.6	Garantiehinweise..................................................................................................................	7
2	Gerätebeschreibung ..........................................................................................................	8
2.1	Systemübersicht ...................................................................................................................	8
2.2	Gesamtansicht....................................................................................................................	10
2.3	Verwendungszweck ...........................................................................................................	11
2.4	Gerätebeschreibung und Hauptmerkmale...........................................................................	11
2.5	Objektive............................................................................................................................	12
3	Inbetriebnahme und Bedienung ....................................................................................	13
3.1	Mikroskop in Betrieb nehmen.............................................................................................	13
3.2	Bedienelemente..................................................................................................................	14
3.3	Mikroskop bedienen...........................................................................................................	16
3.3.1	Okularabstand und Einblickhöhe einstellen .........................................................................	16
3.3.2	Augenfehlsichtigkeit am Okular ausgleichen und Okularzeiger einsetzen ............................	16
3.3.3	Durchlicht-Hellfeld am Mikroskop Full-Köhler einstellen ......................................................	17
3.3.4	Durchlicht-Hellfeld am Mikroskop Fixed-Köhler einstellen ...................................................	18
3.3.5	Durchlicht-Phasenkontrast oder Durchlicht-Dunkelfeld einstellen ........................................	19
3.4	Mikroskop umrüsten ..........................................................................................................	20
3.4.1	Tubus wechseln..................................................................................................................	20
3.4.2	Farbfilter einsetzen .............................................................................................................	20
3.4.3	Halogenlampe 6 V / 30 W bzw. LED-Beleuchtung wechseln................................................	21
3.4.4	Objektiv wechseln ..............................................................................................................	21
3.4.5	Spiegel einund ausbauen..................................................................................................	22
3.4.6	Kamera ansetzen................................................................................................................	23
4	Pflege und Störungsbeseitigung....................................................................................	25
4.1	Gerät pflegen .....................................................................................................................	25
4.2	Störungsbeseitigung...........................................................................................................	26
5	Anhang.............................................................................................................................	27
5.1	Technische Daten ...............................................................................................................	27

Deutsch

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3

Deutsch

Carl Zeiss Hinweise zur Gerätesicherheit Primo Star

1 HINWEISE ZUR GERÄTESICHERHEIT

1.1Allgemeine Sicherheitshinweise

Bitte machen Sie sich vor Inbetriebnahme des Mikroskops mit dem Inhalt dieser Bedienungsanleitung gründlich vertraut.

Ergänzende Informationen erhalten Sie von unserem Service oder von autorisierten Vertretungen.

Zur Gewährleistung einer sicheren Arbeitsweise und ungestörten Funktion des Mikroskops sind unbedingt die in der Bedienungsanleitung angegebenen Vorsichtsmaßnahmen und Warnungen zu beachten.

Diese sind im laufenden Text durch Symbole gekennzeichnet:

VORSICHT

Bei Nichtbeachtung der Sicherheitshinweise besteht eine Gefahr für den Benutzer.

ACHTUNG

Dieses Symbol kennzeichnet eine Gefahr, die für das Gerät oder Gerätesystem entstehen kann!

ACHTUNG

Vor Eingriff in das Mikroskop Steckernetzteil vom Netz trennen!

HINWEIS

Arbeitshinweise, die beim Umgang mit dem Mikroskop zu beachten sind.

1.2	Gerätesicherheit und EMV
Das	Mikroskop Primo Star wurde entsprechend der Norm DIN	EN 61010-1 (IEC 61010-1) und
IEC 61010-2-101 "Sicherheitsbestimmungen für elektrische Mess-,		Steuer-, Regelund Laborgeräte“,
konstruiert, gefertigt und geprüft.

Das Mikroskop Primo Star erfüllt die Anforderungen der EG-Richtlinie 98/79/EG Anhang 1 und ist mit dem - Zeichen gekennzeichnet.

Funkentstörung nach EN 55011 Klasse B

Störfestigkeit nach DIN EN 61326

Die Geräte werden gemäß der WEEE-Richtlinie 2002/96/EG entsorgt.

4	B 46-0071 v 03/06