Zeiss Primo Star User Manual [en, de, fr]

Primo Star

Operating manual

Bedienungsanleitung

Mode d’emploi

Instrucciones de manejo

Инструкция по применению

操作手册

中文

Carl Zeiss Copyright Primo Star

Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning

safe operation of the instrument. The specifications are subject to change; the manual is not covered

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by an update service. Unless expressly authorized, forwarding and duplication of this document, as well as utilization and communication of its contents are not permitted. Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model.

Die Kenntnis dieser Anleitung ist für die Bedienung des Gerätes erforderlich. Bitte machen Sie sich deshalb mit dem Inhalt vertraut und befolgen Sie besonders Hinweise, die den sicheren Umgang mit dem Gerät betreffen. Änderungen im Interesse der technischen Weiterentwicklung bleiben vorbehalten; das Handbuch unterliegt nicht dem Änderungsdienst. Weitergabe sowie Vervielfältigung dieser Unterlage, Verwertung und Mitteilung ihres Inhalts sind nicht gestattet, soweit nicht ausdrücklich zugestanden. Zuwiderhandlungen verpflichten zu Schadenersatz. Alle Rechte für den Fall der Patenterteilung oder Gebrauchsmuster-Eintragung vorbehalten.

L’utilisation de l'appareil suppose la bonne connaissance du présent mode d’emploi. Nous vous prions par conséquent

Fran

de lire attentivement les informations contenues dans ce document et de respecter notamment les consignes relatives à la sécurité d’utilisation. Le fabricant se réserve le droit d’apporter des modifications techniques en fonction de l’évolution des technologies. Ces modifications ne sont pas automatiquement prises en compte dans le mode d'emploi

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qui accompagne chaque appareil. Toute divulgation, reproduction ou publication du présent document, même partielle, est interdite sans notre autorisation écrite. Toute infraction donne droit au versement de dommages et intérêts. Tous les droits sont réservés en cas de délivrance d’un brevet ou de dépôt d’un modèle d'utilité.

El manejo de este equipo presupone el conocimiento de las presentes instrucciones. Por eso le rogamos

familiarizarse con su contenido y observar en particular las indicaciones que se refieren al manejo seguro del mismo. Nos reservamos el derecho a modificaciones en interés del desarrollo técnico; el manual no está sujeto al servicio de

añol

actualización. Sin nuestro consentimiento expreso no se autoriza ni la entrega y reproducción de este manual, ni el aprovechamiento y la comunicación de su contenido. Cualquier contravención implica el pago de una indemnización. Reservados todos los derechos para el otorgamiento de patentes o el registro de modelos de utilidad.

Знание данной инструкции необходимо для использования прибора. Поэтому необходимо ознакомиться

с ее содержанием и особенно следовать указаниям, касающихся безопасного обращения с прибором.

сский

Фирма оставляет за собой право на изменения в интересах технического усовершенствования; руководство не подлежит изменениям. Передача и тиражирование данных документов, использование и сообщение их содержания не допускаются без особого возмещение убытков. Фирма оставляет за собой право на выдачу патента или регистрацию зарегистрированной модели.

版权

操作本仪器必须先阅读本手册内容。因此请务必熟悉本手册内容，尤其要遵从有关仪器正确操作的注意事项。技术可能会有更新，此手册不含升级服务。未经授权禁止复制，利用和转载本手册内容。如有违背必须承担相应赔偿责任。保留所有申请专利或者样品注册权利。

Issued by: Carl Zeiss MicroImaging GmbH

P.O.B. 4041, 37030 Göttingen, Germany Phone: +49 (0) 551 5060 660 Fax: +49 (0) 551 5060 464 E-Mail: micro@zeiss.de

www.zeiss.de

Number of this manual: B 46-0071 v Date of issue: Version 7, 03/01/2006

разрешения. В случае нарушений полагается

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Primo Star Contents Carl Zeiss

CONTENTS

Page

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1 Notes on Instrument Safety.............................................................................................. 4

1.1 General safety notes............................................................................................................. 4

1.2 Instrument safety and EMC .................................................................................................. 4

1.3 Unpacking, transportation, storage....................................................................................... 5

1.4 Disposal................................................................................................................................ 5

1.5 Use....................................................................................................................................... 5

1.6 Notes on warranty................................................................................................................ 7

2 Description ......................................................................................................................... 8

2.1 System overview................................................................................................................... 8

2.2 Total view........................................................................................................................... 10

2.3 Intended use ...................................................................................................................... 11

2.4 Instrument description and main features ........................................................................... 11

2.5 Objectives........................................................................................................................... 12

3 Start-Up and Operation .................................................................................................. 13

3.1 Starting up the microscope................................................................................................. 13

3.2 Controls ............................................................................................................................. 14

3.3 Operating the microscope .................................................................................................. 16

3.3.1 Setting interpupillary distance and viewing height .............................................................. 16

3.3.2 Compensating for ametropia and inserting the eyepiece pointer......................................... 16

3.3.3 Adjusting transmitted-light brightfield on the Full Köhler microscope.................................. 17

3.3.4 Adjusting transmitted-light brightfield on the Fixed Köhler microscope ............................... 18

3.3.5 Adjusting transmitted-light phase contrast or transmitted-light darkfield............................. 19

3.4 Converting the microscope................................................................................................. 20

3.4.1 Changing the tube ............................................................................................................. 20

3.4.2 Inserting color filters........................................................................................................... 20

3.4.3 Replacing the 6V 30W halogen lamp or the LED illumination.............................................. 21

3.4.4 Changing objectives ........................................................................................................... 21

3.4.5 Installing/removing the illuminating mirror.......................................................................... 22

3.4.6 Installing a camera.............................................................................................................. 23

4 Care and Troubleshooting .............................................................................................. 25

4.1 Instrument care .................................................................................................................. 25

4.2 Troubleshooting ................................................................................................................. 26

5 Appendix.......................................................................................................................... 27

5.1 Technical data .................................................................................................................... 27

B 46-0071 v 03/06 3

Carl Zeiss Notes on Instrument Safety Primo Star

1 NOTES ON INSTRUMENT SAFETY

1.1 General safety notes

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Please read this Operating Manual carefully before starting up the microscope. If you need supplementary information, contact Carl Zeiss Service or an authorized agency. To ensure safe operation and troublefree function of the microscope, strictly observe the precautions and

warnings given in this manual. These are set off herein as follows:

CAUTION This symbol indicates a possible hazard to the user of the instrument.

CAUTION This symbol indicates a possible hazard to the instrument or system.

CAUTION Disconnect the plug-in power unit from line power before opening the microscope!

NOTE This symbol refers you to advice that you must observe under all circumstances.

1.2 Instrument safety and EMC

The Primo Star microscope has been designed, produced and tested in compliance with the standards DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 "Safety requirements for electrical equipment for measurement, control and laboratory use “.

The Primo Star microscope meets the requirements of the EC Directive 98/79/EC Annex 1 and carries the

mark.

Radio-noise suppression in compliance with EN 55011 Class B Noise immunity in compliance with DIN EN 61326

The instruments are disposed of in compliance with the WEEE Directive 2002/96/EC.

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Primo Star Notes on Instrument Safety Carl Zeiss

1.3 Unpacking, transportation, storage

Please observe the following safety notes for unpacking, transportation and storage of the microscope:

− The microscope is supplied packed to commercial standards in a plastic case with cardboard

packaging; use the original packaging only for any transportation.

− Retain the original packaging for longer storage or return to the manufacturer.

− When unpacking the equipment, verify that all parts specified on the delivery note are present.

− Keep transport and storage temperatures as specified in Technical Data.

− Set up the microscope on a stable worktable with solid and smooth tabletop.

− Do not touch optical surfaces.

1.4 Disposal

Please observe the following safety notes for the disposal of the microscope:

Defective microscopes should not be disposed of with household waste; dispose of them in compliance with the provisions of the law.

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1.5 Use

The microscope including its original accessories must not be used for microscopic techniques other than those described in this Operating Manual.

Please observe the following safety notes when using the microscope:

The manufacturer cannot assume any liability for other applications, including those of individual modules or single components. This also applies to any service or repair work that is not carried out by authorized service personnel. In case of non-compliance, all warranty claims shall be forfeited.

The microscope may only be operated by trained personnel who must be aware of the possible dangers involved with microscopy and the particular application concerned. The microscope may only be operated if set up on a stable, solid and smooth surface.

The stereomicroscope is a high-precision instrument that can be impaired in its performance or even be destroyed when handled improperly.

B 46-0071 v 03/06 5

Carl Zeiss Notes on Instrument Safety Primo Star

The microscope is equipped with a plug-in power unit allowing line voltages to be used in the

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range between 100 and 240 V ± 10%, 50/60 Hz, without the need for changing the voltage setting on the instrument. The plug-in power unit meets the requirements of Protection Class II (with protective insulation). If its casing is damaged, put the plug-in power unit out of operation. The microscope may be operated only with the supplied plug-in power unit.

If it is determined that protection measures are no longer effective, the instrument must be switched off and safeguarded against inadvertent operation. Please contact a Zeiss service agency or the Carl Zeiss Microscopy Service to repair the instrument.

− Always disconnect the power cable, before opening the instrument and changing the lamp

or LED source.

− Wait for the lamp to cool down before replacing it and do not touch the new bulb.

− The instrument may only be opened by qualified specialists or service staff.

− The operation of the instrument in explosion-risk environments is not allowed.

Make sure to read the safety notes provided with Immersol 518 N

immersion oil.

Immersion oil irritates the skin. Avoid any contact with skin, eyes and clothing. After skin contact, wash the oil off with plenty of water and soap. After eye contact, immediately rinse the eye with plenty of water for at least five minutes. If the irritation persists, consult a specialist. Proper disposal of immersion oil: Take care to ensure that immersion oil does not enter surface water or the sewage system.

The microscope is not equipped with special devices for the protection from substances that are corrosive, potentially infectious, toxic, radioactive, or other substances that could be hazardous to health. Make sure to observe all legal regulations, particularly the relevant national accident prevention regulations when handling such substances.

− Before any transport of the instrument, switch it off and let it cool down (hot surface at the

bottom of the instrument).

− Operate the device only on a hard, non-combustible support.

− The plug-in power unit must not get in contact with moisture.

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Primo Star Notes on Instrument Safety Carl Zeiss

1.6 Notes on warranty

The Primo Star Microscope including its original accessories must not be used for microscopic techniques other than those described in this Operating Manual. The manufacturer cannot assume any liability for other applications.

Please note the following information on warranty for the Primo Star Microscope:

− The manufacturer guarantees that the microscope is free of material or manufacturing defects when

delivered.

− Possible defects must be notified to us immediately and steps be taken to minimize damage.

− If notified of such a defect, the manufacturer is obligated to rectify it at his discretion, either by

repairing the instrument or delivering an intact replacement.

− No guarantee is provided for defects caused by natural wear (wearing parts in particular) and improper

use.

− The instrument manufacturer shall not be liable for damage caused by faulty operation, negligence or

any other tampering with the microscope, particularly the removal or replacement of microscope components, or the use of accessories from other manufacturers.

Unauthorized tampering with the instrument shall lead to a forfeit of all warranty claims.

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Carl Zeiss Description Primo Star

2 DESCRIPTION

2.1 System overview

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Primo Star Description Carl Zeiss

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Carl Zeiss Description Primo Star

2.2 Total view

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1 Eyepiece 2 Binocular head 3 Tube 4 Carrying handle 5 Plug-in power unit 6 Stand 7 ON/OFF switch;

illumination intensity

8 Illumination

module

9a Luminous-field

diaphragm, adjustable

9b Luminous-field

diaphragm, fixed

10a Condenser carrier,

Full Köhler

10b Condenser carrier,

Fixed Köhler

11a Abbe condenser,

Full Köhler

11b Abbe condenser,

Fixed Köhler 12 Objective 13 Microscope stage 14 Objective

nosepiece

Fig. 1 Total view

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Primo Star Description Carl Zeiss

2.3 Intended use

The Primo Star microscope is a universally applicable light microscope primarily designed for the examination of cell and tissue cultures as well as sediments in culture flasks, Petri dishes and microplates.

Typical applications:

Examination of blood and tissue samples from the human body, observation of intracellular processes on living cell cultures, cell-cell interactions, motility, growth, potential measurement, detection of medical drugs, microinjection and in vitro fertilization.

When handling hazardous substances, observe the instructions on intended operation, correct use and statutory safety precautions.

2.4 Instrument description and main features

The Primo Star is a transmitted-light microscope of compact design with a small footprint. Beside the high-resolution, infinity-corrected objectives and the important microscopy techniques, such as

brightfield, darkfield and phase contrast, the microscope is optionally available with a camera port for photo and video documentation.

The major features of the microscope include:

− Modular illumination through 6V 30W halogen lamp, LED illumination or illuminating mirror.

− Integrated fixture for external power supply and cable (incl. cable with multi-standard plug and

country-specific plug adapters)

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− Plastic-coated carrying handle integrated in stand for installation, uninstallation and transport

− Blue, light-intensity indicators installed on both sides and well visible from a distance

− Stand in "Full Köhler" or "Fixed Köhler" design

− Convenient coaxial coarse and fine focusing drive, smoothness of coarse focusing drive being

adjustable

− Mechanical stage 75×30 left/right with specimen holder, stage controls optionally on the right or left

− Space-saving, continuously adjustable illumination module optionally with halogen or LED source

− "Full Köhler" or "Fixed Köhler" Abbe condensers for brightfield, darkfield and Ph 2 phase contrast

− Backward inclined quadruple objective nosepiece with W 0.8 lens thread running on ball bearing

− Infinity-corrected "Plan-ACHROMAT" objectives with magnifications of 4x, 10x, 40x and 100x/Oil for

brightfield, darkfield and Ph 2 phase contrast Ph 2 (40x/Ph2) as well as for oil immersion applications (100x/Oil)

− Binocular tube or binocular phototube (50% vis, 50% doc) with ergonomically favorable tube angle of

30° with adjustable interpupillary distance and viewing height

− 10× focusing eyepieces for field-of-view numbers 18 or 20, suitable for spectacle wearers

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Carl Zeiss Description Primo Star

2.5 Objectives

The objectives are the optical heart of the microscope. The objectives may be labeled as follows:

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Plan-ACHROMAT 10×/0.25 ∞/-.

where: 10× Objective magnification,

With a defined color ring on the objective being assigned to each magnification step (Carl Zeiss color code)

0.25 Numerical aperture

∞ Infinite mechanical tube length

− Usable with cover slip thickness D = 0 or

0.17 mm

0.17 Usable with cover slip thickness D = 0.17 mm

Other labels: Oil Oil immersion objective

Fig. 2 Objective

Ph 2 Phase contrast objective with green

inscription and phase stop Ph 2

Objective magnification multiplied by eyepiece magnification results in overall visual magnification, e.g. 10 x 10 = 100x.

Numerical aperture multiplied by 1000, e.g. 0.25 × 1000 = 250×, presents the maximum useful magnification; there is no resolution for further details above that limit.

When immersion objectives are used, the air between the cover slip and the objective is replaced by a liquid, which in most cases is immersion oil. The plastic oiler containing 5 ml of immersion oil is particularly suitable for this purpose.

Because of their short working distances, the 40×, 40x/Ph 2 and 100x/Oil objectives are equipped with resilient mounts for specimen protection.

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Primo Star Start-Up and Operation Carl Zeiss

3 START-UP AND OPERATION

3.1 Starting up the microscope

Before installing and starting up the microscope, be sure to carefully read and observe the notes on instrument safety (see Section

1).

Do not touch optical surfaces when unpacking the microscope to avoid fingerprints!

The microscope is supplied completely assembled and inclusive of accessories packed to commercial standards. Additionally ordered components, such as sliders or transmitted-light equipment with illuminating mirror, are packed separately.

• Remove the microscope from the transport case and place it onto the worktable. Retain the original packaging for storage of the instrument in longer periods of non-use or for

return to the manufacturer.

• Remove the plug-in power unit (

Fig. 3/2) from

its storage fixture on the back wall of the microscope.

• Replace the installed power outlet adapter by

one of the supplied country-specific adapters

Fig. 3/4), if necessary. To this end, pull off the

( attached adapter and plug on the desired adapter.

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• Connect the plug-in power unit to a power

outlet.

• If the plug-in power unit cannot be plugged

into the chosen power outlet because of limited space, replace the power outlet adapter by the supplied IEC adapter (

Fig. 3/3). This allows the

plug-in power unit to be put flat onto the tabletop and connected to the power outlet through a country-specific appliance cable.

When using the IEC adapter, the plug-in power unit can be fixed to the back wall of the microscope stand by means of the supplied two self-adhesive hooks and the Velcro

• Turn on the microscope with rotary switch (

Fig. 3/5) and adjust the illumination to the desired

Fig. 3 Starting up the microscope

strip.

intensity.

The selected intensity is indicated in five steps by the blue light-emitting diodes (

Fig. 3/1) arranged on

both sides of the stand.

• At the end of the microscopic examinations, turn the microscope off with the rotary switch and cover

it with the dust cover.

• The smoothness of the coarse focusing drive (

Fig. 4/18) is factory-adjusted. However, you can readjust

it when required.

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Carl Zeiss Start-Up and Operation Primo Star

3.2 Controls

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Fig. 4 Controls

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Primo Star Start-Up and Operation Carl Zeiss

Legend to Fig. 4:

1 Eyepieces 2 Binocular body of the tube 3 Illumination-intensity indicators 4 Rotary knob for switch ON/OFF and adjustment of illumination intensity 5 Fine focusing drive (right side) 6 Coarse focusing drive (right side) 7 Control knob for X travel of mechanical stage 8 Control knob for Y travel of mechanical stage

9 Condenser clamp screw 10a Knurled ring for adjustment of luminous-field diaphragm (with Full Köhler equipment only) 10b Luminous-field diaphragm (fixed in Fixed Köhler equipment) 11a Condenser entering screws (in Full Köhler equipment: knurled screws) 11b Condenser centering screws (in Fixed Köhler equipment: Allen screws) 12 Spring lever of specimen holder 13 Knurled ring of objective nosepiece 14 Lever for the adjustment of the aperture diaphragm of the condenser 15 Knurled knob for vertical adjustment of condenser 16 Coarse focusing drive (left side) 17 Fine focusing drive (left side) 18 Knurled ring for adjusting the smoothness of the coarse focusing drive

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Carl Zeiss Start-Up and Operation Primo Star

3.3 Operating the microscope

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3.3.1 Setting interpupillary distance and viewing height

• Swing the eyepiece tubes symmetrically slightly

toward or away from one another to adjust the distance between the tubes to your individual interpupillary distance (

Fig. 5).

The adjustment of the interpupillary distance is correct when you see only one round image while

Fig. 5 Setting the interpupillary distance

looking through the two eyepieces!

• Swivel the eyepiece tubes fully up (

down (

Fig. 6/B) to adjust the viewing height to

Fig. 6/A) or

your individual requirements.

Fig. 6 Adjusting the viewing height

3.3.2 Compensating for ametropia and inserting the eyepiece pointer

The eyepieces ( over rubber eyecups (

Fig. 7/3) are equipped with fold-

Fig. 7/1: pulled out; Fig. 7/2:

folded over). Both eyepieces are suitable for spectacle wearers.

Additionally, they contain a focusing ring for the compensation of defective vision. The provided diopter scale serves to facilitate finding the correct setting.

The eyepiece pointer can be inserted in one eyepiece, when required.

To this end, follow this procedure:

• Use Allen key SW 1 mm to loosen grub screw

(

Fig. 7/6) on the binocular body from the

bottom; remove the eyepiece.

• Unscrew the stop (

Fig. 7/5) by hand from the

eyepiece.

• Insert the eyepiece pointer (

Fig. 7/4) into the

eyepiece (with the coated side facing your eyes). Screw in the eyepiece stop again.

• Put the eyepiece into the tube and fix it with

Fig. 7 Inserting the eyepiece pointer

the grub screw.

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Primo Star Start-Up and Operation Carl Zeiss

• Turn the focusing ring of the eyepiece (Fig. 7/3) to focus on the triangular figure of the eyepiece

pointer.

• Put the specimen onto the mechanical stage. Look at the specimen through the eyepiece with the eyepiece pointer and focus on the microscopic image by turning on the focusing drive.

• When in the above-mentioned eyepiece both microscopic image and eyepiece pointer appear sharply, focus the image for the second eye by turning the focusing ring of the second eyepiece.

Having done so, both microscopic images inclusive of the eyepiece pointer are focused. Afterwards, you should focus on the specimen only by adjusting the focusing drive.

3.3.3 Adjusting transmitted-light

brightfield on the Full Köhler microscope

• First, place a contrasty specimen slide with the

0.17 mm cover slip being on top in the specimen holder of the mechanical stage. Fix the slide by means of the spring lever

Fig. 4/12).

(

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• If the microscope stand is equipped with a phase or dark-filed slider, pull this slider out to the left as far as it will go.

• Turn rotary knob (

Fig. 8/1) on microscope stand

to adjust the illumination intensity.

• Move the Abbe condenser up to the top mechanical stop by turning knurled knob

Fig. 4/15); set the control lever of the aperture

( diaphragm (

Fig. 4/14) to mid-position.

The knurled knob for vertical adjustment of the condenser is at the left of the microscope, if you use the mechanical stage 75x30 with drive on the right, it is at the right, if the stage controls are on the left.

• Turning at the knurled ring (

Fig. 8/6) of the

nosepiece, swing the 10x objective into the light path.

• On the binocular tube (

Fig. 8/7), first look

through one eyepiece and turn the focusing

Fig. 8/2) to focus on the specimen.

drive (

Fig. 8 Adjusting transmitted-light

brightfield

• Then, readjust the focus for the other eye, if necessary, by turning the eye lens of the focusing eyepiece.

• Close the luminous-field diaphragm ( of view (

Fig. 8/A).

Fig. 8/3) until it becomes visible (even if not in focus) in the field

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Carl Zeiss Start-Up and Operation Primo Star

• Turn the knurled knob for vertical adjustment of the condenser (Fig. 4/15) until the edge of the

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luminous-field diaphragm appears sufficiently sharp (

• Center the image of the luminous-field diaphragm using both centering screws ( condenser (

Fig. 8/D).

(

Fig. 8/C). Then, open the diaphragm until it just disappears from the field of view

Fig. 8/B).

Fig. 8/5) of the

• To adjust the aperture diaphragm (contrast), remove one eyepiece from the tube and look through the tube with your naked eye. Swing lever ( 2/3 ... 4/5 of the diameter of the exit pupil of the objective (

Fig. 8/4) to adjust the aperture diaphragm to approximately

Fig. 8/E). In most applications, this

aperture diaphragm setting provides optimum contrast at almost ideal resolution, and is therefore the best compromise for the human eye.

• Insert the eyepiece back in the tube.

Specimen field size and objective aperture change after every objective change. Therefore, repeat the adjustment of luminous-field diaphragm and aperture diaphragm to obtain optimum results.

3.3.4 Adjusting transmitted-light brightfield on the Fixed Köhler microscope

The Primo Star Fixed Köhler Microscope is supplied factory-adjusted. Operation is restricted to a few manipulations.

• Place the specimen in the specimen holder of the mechanical stage.

• If the microscope stand is equipped with a phase or dark-filed slider, pull this slider out to the left as

far as it will go.

• Adjust the desired magnification by swinging in the corresponding objective.

• Set the control lever of the aperture diaphragm of the condenser to the value of the selected

magnification (10x, 40x or 100x).

• Turn the rotary knob on the microscope stand to adjust the illumination intensity to a level pleasant for observation.

If the condenser had been removed (e.g. for installing the illuminating mirror), make sure to reinstall and center it by means of the two centering screws (for this, refer to Section

3.4.5).

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Primo Star Start-Up and Operation Carl Zeiss

3.3.5 Adjusting transmitted-light phase contrast or transmitted-light darkfield

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• First, adjust the microscope as you do for

brightfield.

• Turn the nosepiece to swing the phase-contrast

objective (40x/Ph 2) into the light path.

• Open the luminous-field diaphragm (

Fig. 9/3, if

adjustable) on the stand and open the aperture diaphragm by means of control lever ( on Abbe condenser (

• If the phase-contrast slider (

Fig. 9/2).

Fig. 9/6) was not

Fig. 9/1)

factory-installed yet, first unscrew its screw

Fig. 9/7). Then, push the slider from the left

( into the Abbe condenser and screw the screw

Fig. 9/7) back in again.

(

• Push the slider to the right as far as it will go to

move the phase stop into the light path.

• Move the control lever of the aperture

diaphragm to fully open it on the Full Köhler model or set it to the PH mark on the Fixed Köhler model.

• Adjust the illumination intensity as needed.

Fig. 9 Inserting the slider

• Check the centering of the phase stop

according to the drawing in

Fig. 10. To this end, remove one eyepiece and replace it by the eyepiece telescope.

• Center the phase stop, if necessary, (

Fig. 10/A)

by turning the two adjusting screws of the slider ( SW 1.5 ( corresponds to that shown in

Fig. 9/5) by means of the two Allen keys

Fig. 9/4) until the phase stop image

Fig. 10/B.

• Afterwards, replace the eyepiece telescope by the eyepiece again.

For darkfield applications, use the darkfield slider in place of the phase-contrast slider.

Fig. 10 Centering the phase stop

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Carl Zeiss Start-Up and Operation Primo Star

3.4 Converting the microscope

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Unplug the plug-in power unit from line power before converting the microscope.

3.4.1 Changing the tube

Fig. 11 Changing the tube

• Loosen clamp screw ( installed tube ( approximately 90° ( side take it of upward (

For reasons of space, the tube may

Fig. 11/2), turn the

Fig. 11/1) clockwise by

Fig. 11/A) and on the right

Fig. 11/B).

also be clamped by the grub screw with hexagonal socket head supplied with the tube.

• Insert the tube to be installed, with the eyepieces pointing to the right and its dovetail mount slightly inclined, under the two holding elements (

Fig. 11/4) in the stand.

• Then, place the tube horizontally onto the stand. In doing so, the groove on the underside of the tube must be located above the third holding element (

Fig. 11/3) of the stand.

• Turn the tube counterclockwise by 45° (eyepieces pointing frontward), align it to the stand and tighten clamp screw (

For space-saving storage of the

Fig. 11/2).

microscope (e.g. in a cabinet) the tube may also be rotated by 180° to the back.

3.4.2 Inserting color filters

• Move the condenser carrier fully up by turning the knurled screw for vertical adjustment

Fig. 4/15).

(

• Unscrew the cover cap ( luminous-field diaphragm (

Fig. 12/3) from the

Fig. 12/1).

• Put the desired filter – yellow, green or blue – (

Fig. 12/2) onto the mounting surface of the

luminous-field diaphragm and screw the cover cap back into place.

Fig. 12 Inserting a color filter

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Primo Star Start-Up and Operation Carl Zeiss

3.4.3 Replacing the 6V 30W halogen lamp or the LED illumination

Unplug the plug-in power unit from the power outlet and allow for a sufficient cool-down time of the 6V 30W halogen lamp before you replace it.

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• Loosen both fastening screws (

illumination module (

Fig. 13/1 o 2). In doing so,

Fig. 13/3) of the

slightly press the screws against the spring and turn them by 90°: Turn left screw clockwise and right screw counterclockwise.

• Pull the illumination module (

Fig. 13/1 or 2) out

of the stand.

• If the stand is equipped with a 6V 30W halogen

lamp ( (

Fig. 13/4) from the lamp carrier and insert the

Fig. 13/2), remove the halogen lamp

new halogen lamp. Do not touch the new lamp with naked fingers as this will reduce lamp life.

• If the stand is equipped with an LED source,

replace the complete illumination module including the LED (

• Push the illumination module (

Fig. 13/1).

Fig. 13/1 or 2)

back into the stand and lock it by means of the two screws (

Fig. 13/3). In doing so, slightly press

the screws against the spring and turn them by 90°: Turn left screw counterclockwise and right screw clockwise.

Fig. 13 Replacing the 6V 30W halogen lamp

or the LED illumination module

3.4.4 Changing objectives

• Turn the focusing drive to move the mechanical

stage fully down.

• Turn the nosepiece (

objective to be changed (

Fig. 14/3) to move the

Fig. 14/1) into a lateral

position.

• Unscrew the objective and remove it

downward.

• Screw the desired objective (

nosepiece as far as it will go.

• If you intend to insert the objective in a lens

mount not used so far, remove the dust cover from the corresponding mount of the nosepiece.

Fig. 14/2) into the

Fig. 14 Changing an objective

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Carl Zeiss Start-Up and Operation Primo Star

3.4.5 Installing/removing the

illuminating mirror

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Fig. 15 Removing/installing the condenser

The mirror serves to illuminate the specimen if no power outlet is available.

It can be used only in combination with the Primo Star Fixed Köhler Microscope. For this purpose, it is necessary to remove the condenser and the condenser carrier insert.

Installing the illuminating mirror:

• Remove cover cap ( field diaphragm (

Fig. 15/6) from luminous-

Fig. 15/5).

• Using an Allen key, loosen the clamp screw

Fig. 15/2) of the condenser and the centering

( screws (

Fig. 15/4) so that the condenser (Fig. 15/7) can

(

Fig. 15/3) of the condenser carrier

be removed frontward. Remove the condenser

Fig. 15/7).

(

• Unscrew clamp screw ( from condenser carrier insert (

Fig. 15/2 or Fig. 16/1)

Fig. 15/1 or

Fig. 16/3).

Fig. 16 Installing/removing the illuminating

mirror

• Push the condenser carrier backward against the spring and inclining it remove it upward from the condenser carrier (

• Insert the mirror (

Fig. 16/5) from top through

Fig. 15/4).

the aperture of the condenser carrier and place it onto the mount of the luminous-field diaphragm (

Fig. 16/2). Take care that the mirror

rests level on the mount.

• Rotate and incline the mirror until the daylight is reflected homogeneously into the light path.

Removing the illuminating mirror:

• Remove the mirror (

Fig. 16/5) upwards through

the aperture of the condenser carrier.

• Slightly incline the condenser carrier insert

Fig. 15/1 or Fig. 16/3) to insert it in the

( aperture of the condenser carrier. In doing so, press the unit backward against the spring and place it horizontally onto the condenser carrier.

• Screw the condenser clamp screw ( into the condenser carrier insert.

Fig. 16/1)

22 B 46-0071 v 03/06

Primo Star Start-Up and Operation Carl Zeiss

• Taking care that the locating screw on the rear side of the condenser fits in groove (Fig. 16/4) of the

condenser carrier insert, put the condenser into this insert.

• Screw the cover cap back onto the luminous-field diaphragm.

• To center the condenser, connect the microscope to the power outlet and switch it on.

• Turn the nosepiece to swing the 4x objective into the light path.

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• Turn the two centering screws (

Fig. 15/3) to adjust the illuminated image symmetrically to the edge of

the field of view.

3.4.6 Installing a camera

You can install a digital camera, a video camera or a compact digital camera to your choice on the microscope via the available camera adapters (see Section

• First, install the photo tube (

• Loosen clamp screw (

2.1).

necessary (refer to Section

Fig. 17/8) and remove the

Fig. 17/7), if

3.4.1).

dust cap from the photo tube.

Installing a compact digital camera

• Sliding mount ( M37/52 (

Fig. 17/3) and lens mount (Fig. 17/6)

Fig. 17/4), thread adapter ring

are supplied premounted as Digital Camera Adapter P95 M37/52x0.75. The opposite drawing shows this unit dismantled. Furthermore, you may unscrew the M37/52 thread adapter ( sliding mount (

Fig. 17/3) (not shown) from the

Fig. 17/4) so that you can also

mount cameras with M37 thread.

• Mount the adapter ring ( with the camera) to the camera (

Fig. 17/2) (supplied

Fig. 17/1) (see

the operating instructions of the camera).

Fig. 17 Installing a camera

• Screw the unit consisting of sliding mount ( mount (

Fig. 17/6) into the adapter ring (Fig. 17/2).

Fig. 17/4), thread adapter ring M37/52 (Fig. 17/3) and lens

• Insert the camera with adapter into the photo tube as far as it will go. Align it and fasten it with clamp screw (

Fig. 17/8).

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Carl Zeiss Start-Up and Operation Primo Star

• Depending on the microscope equipment or the camera used, it may be necessary to optimize the

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distance between camera lens and lens mount ( necessary in particular, if it is impossible to obtain an unvignetted image in any of the zoom positions

Fig. 17/6) (see double-headed arrow). This will be

of the camera lens. To this end, adjust the camera as follows:

− Switch off the autofocus.

− Set the object distance to ∞.

− Set aperture-priority auto exposure mode.

− Choose an aperture as large as possible (i.e. small aperture value!).

Not all the cameras provide these options. Please consult the Operating Manual of the used camera.

• Loosen grub screw (

Fig. 17/5).

• Vary the camera lens/lens mount distance gradually, i.e. displace the sliding mount with camera on lens mount in defined steps.

• Zoom the camera lens through from wide angle (W) to tele position (T).

• Carry out this test until the image is format filling without masking or vignetting.

• Retighten the grub screw (

With camera/adapter combinations that have not been recommended expressly by Zeiss, it

Fig. 17/5).

may be quite impossible to obtain an unvignetted image.

Installing a digital camera or video camera with C-mount thread

Cameras with C-mount thread are to be connected to the phototube of the microscope by means of the camera adapter P95-C 2/3” 0.65x or P95-C ½” 0.5x (

• Put the camera (

Fig. 17/10) together with the corresponding camera adapter (Fig. 17/9) into the

phototube as far as it will go, align it and fasten it by means of the clamp screw (

Fig. 17/9).

Fig. 17/8).

24 B 46-0071 v 03/06

Primo Star Care and Troubleshooting Carl Zeiss

4 CARE AND TROUBLESHOOTING

4.1 Instrument care

Care of the microscope is restricted to the following operations:

• Cover the instrument with the dust cover after every use.

• Do not install the microscope in a humid room; maximum humidity < 75%.

• Cover open tubes with the dust caps.

• Remove dust and loose dirt from visible optical surfaces with a brush, blower brush, cotton bud,

optics cleaning tissue, or a cotton cloth.

• Remove water-soluble dirt (coffee, cola, etc.) by blowing on it and subsequent wiping it off with a cloth moistened with water to which you may also add a mild detergent.

• Wipe off stubborn oily or fatty dirt (immersion oil, fingerprints) with a cotton bud or a lint-free cotton cloth and the optics cleaning solution L. The cleaning solution consists of 90 vol% gasoline and 10 vol% isopropanol (IPA). Its individual constituents are also known as: Gasoline: Medical alcohol, petrolether Isopropanol: 2-Propanol, Dimethylcarbinol, 2-Hydroxypropane

Clean optical surfaces by polishing in circles starting in the middle and moving to the edges using slight pressure only.

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For the use in humid climatic zones, all optical components of the microscope are provided with protection against fungus attack.

B 46-0071 v 03/06 25

Carl Zeiss Care and Troubleshooting Primo Star

4.2 Troubleshooting

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The field of view is not completely visible.

Problem Cause Remedy

Nosepiece with objective has not been switched into click-stop position.

Condenser has not been set correctly. Set condenser correctly.

Switch nosepiece with objective into click-stop position.

Low resolving power, poor image contrast

The aperture diaphragm has not been adjusted correctly.

The luminous-field diaphragm has not been adjusted correctly.

The filter has not been inserted correctly in the filter mount.

The aperture diaphragm has not been opened to the correct size.

Condenser not focused correctly. Focus the condenser.

Wrong cover slip thickness selected for use of transmitted-light objectives corrected for 0.17mm cover slips.

Use of no or non-specified immersion oil with immersion objective

Air bubbles in immersion oil Remove the bubbles by applying new oil

Immersion oil on the front lens of a dry objective.

Dirt or dust on the optical surfaces of objectives, eyepieces, condenser, or filters

Adjust aperture diaphragm correctly.

Adjust luminous-field diaphragm correctly.

Insert filter correctly into filter mount.

Set opening of aperture diaphragm to correct size.

Use standard 0.17 mm cover slips.

Use the supplied immersion oil.

or moving the objective to and fro.

Clean the front lens of the dry objective.

Clean the respective optical components.

Greater focus differences after changing the objective

the LED source does not light up although the microscope has been switched on.

The 6V 30W halogen lamp is flickering, its light intensity unstable, the illumination inhomogeneous

Stage comes down by itself, image focus is unstable

The focusing eyepieces have not been set correctly.

Power plug has not been plugged into power outlet.

6V 30W halogen lamp or LED source is

defective. End of average service life of 6V 30W

halogen lamp has been reached.

Power cable Incorrectly installed or broken power cable

The pins of the 6V 30W halogen lamp have not been inserted correctly in the lamp holder.

The pins of the 6V 30W halogen lamp have not been inserted symmetrically in the lamp holder.

Adjusted torque of coarse focusing drive is too low.

Set the focusing eyepieces to the corresponding defective vision.

Connect power plug to power outlet. The 6V 30W halogen lamp or

Replace the defective 6V 30W halogen lamp or the LED module

Replace the 6V 30W halogen lamp.

Connect the power cable correctly or replace it.

Insert the pins of the 6V 30W halogen lamp correctly in the lamp holder.

Insert the pins of the 6V 30W halogen lamp symmetrically in the lamp holder.

Increase the torque of the coarse focusing drive to make motion stiffer.

26 B 46-0071 v 03/06

Primo Star Appendix Carl Zeiss

5 APPENDIX

5.1 Technical data

Dimensions (width x depth x height)

Stand with binocular tube Stand with phototube With tube / phototube turned by 180°

approx. 190 x 425 x 395 mm approx. 190 x 425 x 395 mm approx. 190 x 375 x 395 mm

Weight

Primo Star with phototube 8.2 kg

Ambient conditions

Transport (in packaging):

Permissible ambient temperature

Storage:

Permissible ambient temperature Permissible humidity (no condensation)

Operation:

Permissible ambient temperature Permissible humidity (no condensation) Atmospheric pressure

-40 to +70 °C

+10 to +40 °C max. 75% at 35 °C

+5 to +40 °C max. 75% at 35 °C

800 hPa to 1060 hPa

Operating data

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Protection Class Protection Type Electrical safety

Pollution degree Overvoltage category Radio interference suppression Line voltage

Line frequency Power consumption Output of plug-in power unit Microscope 12V/6V DC

Light sources

Halogen lamp

Adjustability of light source Color temperature at 6V Luminous flux Average service life Luminous area

II IP20 in compliance with DIN EN 61010-1 (IEC 61010-1) including CSA and UL directives 2 II in accordance with EN 61326 100 to 240 V (±10 %) wide-range input power supply, i.e. the voltage setting of the instrument need not be changed! 50 / 60 Hz 70 VA; secondary voltage of external power supply 12V 12 V DC; max. 2.5A

1.5V – 6V adjustable

HAL 6V 30W continuous, from 1.5 to 6V DC 2800 K 280 lm 1000 h

1.5 x 3 mm

B 46-0071 v 03/06 27

Carl Zeiss Appendix Primo Star

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Constant, brightness-independent color temperature of Homogeneous field illumination Suitable for objectives with magnifications of Analogous brightness adjustment from

LED illumination

Optical/mechanical data

Stand with stage focusing

With coarse focusing drive With fine focusing drive Total stage lift

Objective change Manual via quadruple objective nosepiece

Objectives Infinity-corrected objective range with W 0.8 mounting thread

Eyepieces

With field-of-view number 18 With field-of-view number 20

Specimen stage

Dimensions (width x depth) Stage travel (X x Y) Coaxial drive Vernier scales Specimen holder

7480 K 20 mm diameter

4x to 100x approx. 15 to 100%

4 mm/revolution

0.5 mm/revolution 15 mm

30 mm tube size PL 10x/18 Br. foc. PL 10x/20 Br. foc.

Mechanical stage 75x30 right/left 140 x 135 mm 75 x 30 mm Optionally right or left Readable from the right With spring lever, left

Abbe condenser 0.9/1.25, fixed collector For V

Abbe condenser 0.9/1.25 For V

Binocular tube 30°/20

Maximum field-of-view number Interpupillary distance Tube angle Viewing height Viewing port

Binocular phototube 30°/20

Maximum field-of-view number Interpupillary distance Tube angle Viewing height Viewing port Photo/video port Light path selection with swing-in prism

. 4x to 100x

obj

. 4x to 100x

obj

20 Adjustable from 48 to 75 mm 30° 380 to 415 mm Tube factor 1x

20 Adjustable from 48 to 75 mm 30° 380 to 415 mm Tube factor 1x Tube factor 1x, 60 mm mount 50% vis / 100% doc

Illuminating mirror With plane surface and spherical surface with f' = 75 mm

28 B 46-0071 v 03/06

Primo Star Inhaltsübersicht Carl Zeiss

INHALTSÜBERSICHT

Seite

1 Hinweise zur Gerätesicherheit ......................................................................................... 4

1.1 Allgemeine Sicherheitshinweise ............................................................................................ 4

1.2 Gerätesicherheit und EMV.................................................................................................... 4

1.3 Auspacken, Transportieren, Lagern....................................................................................... 5

1.4 Entsorgen............................................................................................................................. 5

1.5 Bedienen.............................................................................................................................. 5

1.6 Garantiehinweise.................................................................................................................. 7

2 Gerätebeschreibung.......................................................................................................... 8

2.1 Systemübersicht ................................................................................................................... 8

2.2 Gesamtansicht.................................................................................................................... 10

2.3 Verwendungszweck ........................................................................................................... 11

2.4 Gerätebeschreibung und Hauptmerkmale........................................................................... 11

2.5 Objektive............................................................................................................................ 12

Deutsch

3 Inbetriebnahme und Bedienung .................................................................................... 13

3.1 Mikroskop in Betrieb nehmen............................................................................................. 13

3.2 Bedienelemente.................................................................................................................. 14

3.3 Mikroskop bedienen........................................................................................................... 16

3.3.1 Okularabstand und Einblickhöhe einstellen......................................................................... 16

3.3.2 Augenfehlsichtigkeit am Okular ausgleichen und Okularzeiger einsetzen............................ 16

3.3.3 Durchlicht-Hellfeld am Mikroskop Full-Köhler einstellen ...................................................... 17

3.3.4 Durchlicht-Hellfeld am Mikroskop Fixed-Köhler einstellen ................................................... 18

3.3.5 Durchlicht-Phasenkontrast oder Durchlicht-Dunkelfeld einstellen ........................................ 19

3.4 Mikroskop umrüsten .......................................................................................................... 20

3.4.1 Tubus wechseln.................................................................................................................. 20

3.4.2 Farbfilter einsetzen ............................................................................................................. 20

3.4.3 Halogenlampe 6 V / 30 W bzw. LED-Beleuchtung wechseln................................................ 21

3.4.4 Objektiv wechseln .............................................................................................................. 21

3.4.5 Spiegel ein- und ausbauen.................................................................................................. 22

3.4.6 Kamera ansetzen................................................................................................................ 23

4 Pflege und Störungsbeseitigung.................................................................................... 25

4.1 Gerät pflegen..................................................................................................................... 25

4.2 Störungsbeseitigung........................................................................................................... 26

5 Anhang............................................................................................................................. 27

5.1 Technische Daten ............................................................................................................... 27

B 46-0071 v 03/06 3

Carl Zeiss Hinweise zur Gerätesicherheit Primo Star

1 HINWEISE ZUR GERÄTESICHERHEIT

1.1 Allgemeine Sicherheitshinweise

Bitte machen Sie sich vor Inbetriebnahme des Mikroskops mit dem Inhalt dieser Bedienungsanleitung gründlich vertraut.

Ergänzende Informationen erhalten Sie von unserem Service oder von autorisierten Vertretungen.

Deutsch

Zur Gewährleistung einer sicheren Arbeitsweise und ungestörten Funktion des Mikroskops sind unbedingt die in der Bedienungsanleitung angegebenen Vorsichtsmaßnahmen und Warnungen zu beachten.

Diese sind im laufenden Text durch Symbole gekennzeichnet:

VORSICHT Bei Nichtbeachtung der Sicherheitshinweise besteht eine Gefahr für den Benutzer.

ACHTUNG Dieses Symbol kennzeichnet eine Gefahr, die für das Gerät oder Gerätesystem entstehen kann!

ACHTUNG Vor Eingriff in das Mikroskop Steckernetzteil vom Netz trennen!

HINWEIS Arbeitshinweise, die beim Umgang mit dem Mikroskop zu beachten sind.

1.2 Gerätesicherheit und EMV

Das Mikroskop Primo Star wurde entsprechend der Norm DIN EN 61010-1 (IEC 61010-1) und IEC 61010-2-101 "Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und Laborgeräte“, konstruiert, gefertigt und geprüft.

Das Mikroskop Primo Star erfüllt die Anforderungen der EG-Richtlinie 98/79/EG Anhang 1 und ist mit dem

- Zeichen gekennzeichnet.

Funkentstörung nach EN 55011 Klasse B Störfestigkeit nach DIN EN 61326

Die Geräte werden gemäß der WEEE-Richtlinie 2002/96/EG entsorgt.

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