Zeiss Standard 25 ICS User Manual

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Standard 25

ICS

Transmitted Light

Microscope

Operating Manual

Standard 25

ICS

Knowledge of this manual is required for the operation of the instrument. Would you ther efore please make yourself familiar with the contents of t his manual and pay special att ention to hint s concerning the safe operation of the instrument.

The specifications are subject to change; the manual is not covered by an update service. © Unless expressly authorized, forw arding and duplic ation of t his document , and the utiliz ation and

communication of its contents are not permitted. Violations will entail an obligation to pay compensation.

Issued by:

Carl Zeiss Mikroskopie

D-07740 Jena Telephone: (**49) 03641 / 64-1616 Telefax: (**49) 03641 / 64-3144 Internet: mikro@zeiss.de

http://www.zeiss.de

Number of this manual: B 40-029 e Date of issue: 01/98

II B 40-029 e 01/98

Standard 25

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CONTENTS

Page

INTRODUCTION........................................................................................................................I

Title page...................................................................................................................................I

Copyright.................................................................................................................................. II

Contents...................................................................................................................................III

Notes on instrument safety.......................................................................................................IV

Warranty notes ........................................................................................................................VI

Overall view ............................................................................................................................VII

1 DESCRIPTION........................................................................................................................1-3

1.1 Name and intended application ..............................................................................................1-3

1.2 Instrument description and main features................................................................................1-3

1.3 Microscope configurations and modules .................................................................................1-4

1.4 Objectives ..............................................................................................................................1-6

1.5 Eyepieces ...............................................................................................................................1-7

1.6 Stage micrometers and eyepiece reticles .................................................................................1-8

1.7 Technical Data......................................................................................................................1-10

2 START-UP .............................................................................................................................2-3

2.1 Unpacking the instrument......................................................................................................2-3

2.2 Attachment of binocular tube 45°/20 ICS or binocular phototube 35°/20 ICS...........................2-3

2.3 Screwing-in of objectives........................................................................................................2-3

2. Insertion of eyepieces.............................................................................................................2-4

2.4.1 Insertion of eyepiece reticle.....................................................................................................2-4

2.4.2 Compensation of ametropia when eyepiece reticles are used ..................................................2-5

2.5 Setting of interpupillary distance and viewing height...............................................................2-5

2.6 Attachment of specimen holder to mechanical stage 75x30 R.................................................2-6

2.7 Attachment of condensers......................................................................................................2-7

2.7.1 Attach condenser 0.9 Z ..........................................................................................................2-7

2.7.2 Attach condenser II Z 0.9 Ph1, 2, 3 and D (0.6/0.9) .................................................................2-7

2.8 Connecting the instrument to the line.....................................................................................2-8

B 40-029 e 01/98 III

Standard 25

ICS

3 OPERATION..........................................................................................................................3-3

3.1 Switch on the instrument.......................................................................................................3-3

3.2 Setting of transmitted-light brightfield for KÖHLER illumination...............................................3-4

3.3 Setting of transmitted-light phase contrast............................................................................. 3-6

3.4 Setting of transmitted-light darkfield......................................................................................3-8

3.5 Setting of transmitted light polarization contrast..................................................................... 3-9

3.6 Measurement of lengths...................................................................................................... 3-10

3.7 Attachment of photomicrography equipment....................................................................... 3-11

3.7.1 Attachment of SLR camera, e.g. CONTAX 167 MT................................................................ 3-11

3.7.2 Attachment of MC 80 DX microscope camera (35 mm film cassette)..................................... 3-13

3.8 Attachment of adapters for video cameras............................................................................3-14

3.9 Insertion of 8x drawing eyepiece.......................................................................................... 3-16

4 CARE, MAINTENANCE AND TROUBLESHOOTING............................................................... 4-3

4.1 Care and maintenance of the instrument................................................................................4-3

4.2 Troubleshooting..................................................................................................................... 4-4

4.2.1 Changing the fuses................................................................................................................4-4

4.2.2 Changing the 6 V 20 W halogen lamp.................................................................................... 4-5

4.2.3 Troubleshooting table ...........................................................................................................4-6

4.3 Table of spares, wearing parts and tools .............................................................................. 4-10

4.4 Requesting service................................................................................................................4-11

Annex...................................................................................................................................A-1

List of abbreviations ...............................................................................................................A-3

Physical and technical units.....................................................................................................A-4

Certification in accordance with DIN EN ISO 9001 / DIN EN 46001...........................................A-5

EC conformity declaration......................................................................................................A-7

IV B 40-029 e 01/98

Standard 25

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Notes on instrument safety

The Standard 25

microscope was designed, produced and tested in compliance with DIN 61010-1

ICS

(IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and meets the requirements of appendix I of directive 73/ 23/EC and the relevant CSA and UL directives. The microscope meets the requirements of the EC direct ive 89/336/EC and the EMC legislation of November 9, 1992. This operation manual includes information and warnings which must be observed by the user.

The following warning and information symbols are used in this manual:

NOTE

This symbol (hand) is a warning which you must observe under all circumstances.

CAUTION

This symbol (warning triangle) is a warning which indicates a hazar d to the operation of the instrument

CAUTION

Disconnect the instrument from the line!

B 40-029 e 01/98 V

Standard 25

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The Standard 25

microscope, including its original accessories, may only be used for the microscope

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techniques described in this manual.

Particular attention must be paid to the following warning notes:

The Standard 25

microscope is categorized as Protection Class I and has been allocated the

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protection degree IP 20. The power plug must be inserted in a properly installed socket featuring a grounding contact. The grounding effect must not be made ineffective by an extension cable which does not have a protective ground wire.

Before switching on the instrument, check whether it is suitable for the line voltage present. Admissible voltage: 100...240 V AC, 50...60 Hz (depending on the model).

Always disconnect the instrument from the line before opening the instrument, before changing the voltage and before changing the fuses.

When changing the instrument fuses, make sure to use only those of the rated power required and the type indicated. The use of makeshift fuses and the short-circuiting of the fuse holders are not permitted.

If it is established that the protection measures are no longer ef fective, t he instrument must be switched off and safeguarded against inadvertent operation.

Dust and dirt can impair the performance of the instrument. Therefore, protect the instrument against these influences as far as possible. If the microscope will not be used for longer periods of time, it must be protected using the dust cover.

The instrument must be operated by trained personnel only who must be aware of the possible danger involved with microscopy and the relevant application.

VI B 40-029 e 01/98

Standard 25

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The Standard 25 performance or damaged when handled improperly.

Notes on warranty:

The manufacturer guarantees that the instrument has no material and production defects when delivered. You must inform us of any defects immediately and we must do anything to minimize the damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision whether he does this by re pairing the instr ument or by deliver ing an instrument free of any defect. No guarantee is provided for defects caused by natural wear (wearing parts in particular).

microscope is an optical precision instrument which can be impair ed in its

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The instrument manufacturer is not liable for damage caused by f aulty operation, negligence or any other meddling with the instrument, or the use of accessories from other manufacturers. This forfeits all the claims against warranty.

With the exception of the work specified in this manual, no maintenance or repair of the Standard 25

may be performed. Repairs may only be performed by Zeiss service staff or

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specially authorized personnel. Should any defect occur with the instrument, please get in touch with your local Zeiss agency first.

B 40-029 e 01/98 VII

Standard 25

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Overall view of the Standard 25

microscope with polarization equipment

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VIII B 40-029 e 01/98

Standard 25

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DESCRIPTION

Contents

1 DESCRIPTION.................................................................................................................1-3

1.1 Name and intended application........................................................................................1-3

1.2 Instrument description and main features.........................................................................1-3

1.3 Microscope configurations and modules...........................................................................1-4

1.4 Objectives........................................................................................................................1-6

1.5 Eyepieces.........................................................................................................................1-8

1.6 Stage micrometers and eyepiece reticles...........................................................................1-8

1.7 Technical Data...............................................................................................................1-10

List of illustrations

Fig. 1-1 Microscope modules........................................................................................................1-2

Fig. 1-2 Standard 25

microscope configurations.......................................................................1-4

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B 40-029 e 01/98 1-1

Standard 25

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Fig. 1-1 Microscope modules

1 Eyepieces 5 Condenser carrier with condenser 2 Binocular tube 6 Mechanical stage with specimen holder 3 Stand 7 Objective 4 Coaxial coarse and fine drive 8 Objective nosepiece

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Standard 25

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1 DESCRIPTION

1.1 Name and intended application

Manufacturer's name:Standard 25 ICS microscope The Standard 25

forms in biology and medicine. These are typical application fields of the Standard 25



laboratory microscopy



doctor's offices



training (schools and universities).

microscope is a universal microscope for the visualization of fine structures and

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1.2 Instrument description and main features

Thanks to its pyramid design, the Standard 25

is a compact transmitted-light microscope. In addition

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to high-resolution ICS objectives and the major brightfield, darkfield, phase contrast and polarization contrast techniques, an optional camera port for photo and video documentation is also available to the user.

Major instrument features:



Sturdy and convenient stand in the pyramid design.



User-friendly coaxial coarse and fine drive



Mechanical stage 75x30 R with ceramic-coated stage surface and specimen holder



Space-saving and continuously adjustable, integrated illuminator with long-life 6V 20W halogen lamp.



Swing-in 0.9 Z condenser for brightfield, and Ph condenser II Z 0.9 for br ightfield and phase contrast Ph1, 2 and 3.



Ball-bearing, 5-position nosepiece with W 0.8" thread.



ICS objectives in the price/performance categories CP-Achromat, A-Plan and Achroplan.



Binocular tube 45°/20 ICS with a viewing height of approx. 440 mm, and binocular photot ube 35°/ 20 with sliding prism 100 obs /100 doc.



10x eyepieces for the field numbers 20 or 18, suitable for spectacle wearers, fixed and adjustable.

B 40-029 e 01/98 1-3

1.3 Microscope configurations and modules

Standard 25

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Fig. 1-2 Standard 25

microscope configurations with polarization equipment

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1-4 B 40-029 e 01/98

Standard 25

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Cat. No.

Configurations

Standard 25

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binocular microscope for transmitted-light brightfield and phase contrast using CP Achromat objectives 10x, 40x Ph2 and 100x "Standard 25

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" binocular microscope for transmitted-light brightfield using objectives "A-Plan" 10x, 40x and 100x "Standard 25

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" binocular microscope for transmitted-light brightfield and phase contrast using objectives "A-Plan" 10x, 40x Ph 2 and 100x "Standard 25

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" binocular microscope for transmitted-light brightfield and phase contrast using objectives "A-Plan" 10x, 20x Ph 2 and 40x Ph 2 "Standard 25

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" binocular microscope for transmitted-light brightfield and phase contrast using objectives "A-Plan" 10x Ph 1, 40x Ph 2 and 100x Ph 3 "Standard 25

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" binocular microscope for transmitted-light brightfield using objectives "A-Plan" 10x, 40x and 100x and phototube "Standard 25

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" binocular microscope for transmitted-light brightfield and phase contrast using objectives "A-Plan" 10x, 40x Ph 2 and 100x and phototube "Standard 25

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" binocular microscope for transmitted-light brightfield and phase contrast using objectives "A-Plan" 10x Ph 1, 40x Ph 2 and 100x Ph 3 and phototube

490841 9804 490841 9904 490845 9804 490845 9904 490846 9804 490846 9904 490847 9804 490847 9904 490848 9804 490848 9904 490865 9804 490865 9904 490866 9804 490866 9904 490868 9804 490868 9904

Modules

1.1 "Standard 25

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" microscope stand with mechanical stage 75x30 R, 5-position nosepiece W 0.8, including integrated illuminator stabilized power supply 220 ... 240 V/50 ... 60 Hz / 45 VA 450815 9902

1.1.1 Line cable with European plug

1.2 "Standard 25

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" microscope stand with mechanical stage 75x30 R, 5-position nosepiece W 0,8, including integrated illuminator stabilized power supply 100 ... 127 V / 50 ... 60 Hz / 45 VA 450816 9902

1.2.1 Line cable with American flat plug

1.3 Long-life 6 V 20 W halogen lamp (spare lamp) 380079 9690

1.4 Conversion filter CB 3; d=32x2 mm 467852

1.5 Conversion filter CB 12, d=32x2 mm 467850 9901

1.6 Interference wide-band filter, green, d=32x4 mm 467803

2.1 Binocular tube 45°/20

2.2 Binocular phototube 35°/20

ICS

ICS (

100% obs / 50% obs : 50% doc) 452929

452928

3.1 Swing-in 0.9 Z condenser 445211

3.2 Phase stop carrier Ph 2 for 445211 470864

3.3 Phase contrast condenser II Z 0.9 Ph 1, 2, 3 and D (0.6/0.9) 445210 not shown Dry darkfield condenser 0.7 - 0.85 and Z condenser holder 465506, 445215 not shown Dry darkfield condenser 0.8 - 0.95 and Z condenser holder 465505, 445215 not shown Ultra-condenser 1.2 - 1.4 and Z condenser holder 465500, 445215

3.4 Diopter d=30 mm 444020

4.1 Dust cover K 459300

4.2 Dust cover G (if binocular phototube is used) 459306 All the above microscope configurations with catalogue numbers ending in 9804 feature the line voltage 230 V,

and those ending in 9904 have a line voltage of 115 V.

B 40-029 e 01/98 1-5

Standard 25

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1.4 Objectives

The objectives are the optical centerpiece of the microscope. The following is an example of how objectives can be labelled:

CP ACHROMAT 10x/0.25 /where

10x = objective magnification, with a defined color ring on the objective being allocated to each

magnification step (Zeiss color code)

0.25 = numerical aperture



= infinite tube length

- : can be used with cover slip thickness D = 0 or 0.17 or

0.17 = can be used with cover slip thickness D = 0.17 and Oil = oil immersion objective Ph 2 = phase contrast objective with a green color ring and phase stop Ph 2

The objective magnification multiplied by the eyepiece magnification (minimum 10x) results in the visual overall magnification, e.g. 10 x 10 = 100x.

The numerical aperture x 1000, e.g. 0.25x1000 = 250x, is the highest useful magnification, i.e. no further details are resolved above that limit.

The objective labeling  indicates that these objectives may only be used with microscopes featuring an infinite tube length and not with instrument s the objectives of which are marked with "160" as their mechanical tube length.

The exact observance of the cover slip thickness of 0.17 mm is all the more necessary the higher the numeric aperture of the objective. Therefore, so-called "Corr" objectives can be set for different cover slip thicknesses via a correction ring. For this, a specimen area is searched, and the position of the correction ring where optimum focus and image contrast are obtained is determined (refocusing is always required).

Immersion objectives are always insensitive to differences in cover slip thickness. When immersion objectives are used, the air between the cover slip and the objective is replaced with a

liquid, which is immersion oil in most cases. The plast ic oiler containing 20 ml of 581 N immersion oil is particularly suitable for this purpose.

Due to their short working distance, objectives 25x and higher feature resilient mounts (specimen protection). To prevent oil contamination of the specimen when the nosepiece is turned, the resilient mounts of the immersion objectives can be locked in their lifted position by turning them to the r ight (do not forget to unlock them again!).

1-6 B 40-029 e 01/98

Standard 25

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The following objectives are available for the Standard 25

Microscopy Technique

Transm.-light brightfield CP-Achromat 5x/0.12 11.2 - 440920

Phase contrast CP-Achromat 10x/0.25 Ph 1 5.1 - 440931

Transm-light brightfield A-Plan 5x/0.12 9.9 - 441020

Phase contrast A-Plan 10x/0.25 Ph 1 4.4 - 441031

Transm.-light brightfield Achrostigmat 20x/0.45 1.6 0.17 440140

Objective Magnification/Num.

Aperture

CP-Achromat

CP-Achromat 10x/0.25 5.1 - 440930 CP-Achromat 40x/0.65 0.3 0.17 440950 CP-Achromat 100x/1.25 Oil 0.07 0.17 440980

CP-Achromat 40x/0.65 Ph 2 0.3 0.17 440951 CP-Achromat 100x/1.25 Oil Ph 2 0.07 0.17 000000 1007 159 CP-Achromat 100x/1.25 Oil Ph 3 0.07 0.17 440981

A-Plan

A-Plan 10x/0.25 4.4 - 441030 A-Plan 20x/0.45 0.53 0.17 441040 A-Plan 40x/0.65 0.43 0.17 441050 A-Plan 100x/1.25 Oil 0.22 0.17 441080

A-Plan 20x/0.45 Ph 2 0.53 0.17 441041 A-Plan 40x/0.65 Ph 2 0.43 0.17 441051 A-Plan 100x/1.25 Oil Ph 3 0.22 0.17 441081

Achrostigmat

Achrostigmat 40x/0.85 Oil 0.28 0.17 440250

Free working distance in mm

microscope:

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Cover slip thickness D in mm

Cat.No.

Phase contrast Achrostigmat 20x/0.45 Ph 2 1.6 0.17 440141

LD-Achrostigmat 20x/0.30 Ph 1 1.0...2.2 0...2.0 440147 LD-Achrostigmat 32x/0.40 Ph 1 1.5...2.2 0.5...1.5 440149

Achroplan

Transm.-light brightfield Achroplan 4x/0.10 11.1 - 440020

Achroplan 10x/0.25 4.8 - 440030 Achroplan 20x/0.45 2.07 0.17 440040 Achroplan 40x/0.65 0.59 0.17 440050 Achroplan 50x/0.90 Oil 0.29 0.17 440057 Achroplan 63x/0.80 0.29 0.17 440060 Achroplan 63x/0.95 0.15 0

(no cover slip) Achroplan 100x/1.25 Oil 0.19 0.17 440080 Achroplan 100x/1.25 Oil Iris 0.19 0.17 440086

Phase contrast Achroplan 10x/0.25 Ph 1 4.8 - 440031

Achroplan 20x/0.45 Ph 2 2.07 0.17 440041 Achroplan 40x/0.65 Ph 2 0.59 0.17 440051 Achroplan 100x/1.25 Oil Ph 3 0.19 0.17 440081

Plan-Neofluar

Transm.-light brightfield Plan-Neofluar 2.5x/0.075 9.3 - 440310

440068

B 40-029 e 01/98 1-7

1.5 Eyepieces

The following eyepieces are offered for the Standard 25 ICS:

Eyepiece Image Angle Cat. No.

Eyepiece PL 10x/18 Br. 39° 444131 9901 Eyepiece PL 10x/18 Br. foc. 39° 444132 9901 Eyepiece E-PL 10x/20 Br. 43° 444231 9901 Eyepiece E-PL 10x/20 Br. foc. 43° 444232 9902 Eyepiece PL 16x/16 Br. 54° 444053 Eyepiece PL 16x/16 Br. foc. 54° 444054

If required, eyecups for the eyepieces can be ordered under the Cat. No. 444801.

Standard 25

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1.6 Stage micrometers and eyepiece reticles

Measuring and counting using the microscope requires stage micrometers and eyepiece reticles, a sm all selection of which is listed below:

Illustration Description, Technical Data Cat. No.

Stage micrometer, positive 5 + 100/100 y D

474026 = 0.17 mm gradation on the + y-axis: 5 mm in 5 intervals; gradation on the - y-axis: 1 mm in 100/100 mm = 10 m, accuracy 1m

Crossline micrometer disk 14:140 / d =26

454060

gradation length=14 mm increments = 0.1mm gradation tolerance  0.001 mm

1-8 B 40-029 e 01/98

Standard 25

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Crossline disk / d = 26 mm 474064

Crossline micrometer disk 10:100 / d =26 mm

gradation length=10 mm increments = 0.1mm gradation tolerance  0.001 mm

Net micrometer 12.5x12.5/5;10 / d = 26 mm

area 12.5x12.5 mm, divided in fields of 5x5 or 10x10.

Photo reticle MC 2.5x / d = 26 mm

for 35 mm photography with an additional magnification of 2.5x or for large-format photography with a 10x additional magnification.

474066 9901

474068

454075

If an eyepiece reticle is used, the binocular tube or the photot ube must be equipped with two foc. eyepieces containing an adjustable eyelens, into one of which the eyepiece reticle is mounted.

B 40-029 e 01/98 1-9

Standard 25

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1.7 Technical Data (1) Dimensions (width x depth x height)

Stand with binocular tube..........................................................................approx. 200 x 350 x 450 mm

Stand with phototube ...............................................................................approx. 200 x 375 x 460 mm

Overall height including T2 adapter and CONTAX 167 MT camera housing..................... approx. 570 mm

(2) Weight

Standard 25

with binocular tube.................................................................................. approx. 6.7 kg

ICS

(3) Ambient conditions Storage and transport (in packaging):

Permissible ambient temperature......................................................................................-40 to + 70° C

Permissible relative humidity (without condensation).............................................................max. 100 %

Operation:

Permissible ambient temperature..................................................................................... +10 to + 35° C

Permissible relative humidity (without condensation)...............................................................max. 85 %

Altitude.............................................................................................................................. max. 2000 m

Atmospheric pressure............................................................................................. 800 hPa to 1060 hPa

(4) Operating data

Operation........................................................................................................................... closed rooms

Protection class.......................................................................................................................................I

Enclosure protection........................................................................................................................IP 20

Electrical safety.................................................................in compliance with DIN EN 61010 (IEC 1010-1)

...............................................................................................................including CSA and UL directives

Pollution degree................................................................................................................................... 2

Overvoltage category.............................................................................................................................II

Radio interference suppression.......................................................in accordance with EN 55011, Class B

Line voltage...................................................................................................... 100 to 127 V AC (10%)

or 220 to 240 V AC (10%)

Line frequency...................................................................................................................... 50 to 60 Hz

Label of voltage range...............................................................................................see instrument rear

Power consumption...............................................................................................................max. 45 VA

Output voltage................................................................................stabilized, adjustable from 1.5 to 6 V

1-10 B 40-029 e 01/98

Standard 25

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(5) Fuses according IEC 127:

for 230 V........................................................................................................T 0.2 A; 250 V; 5 x 20 mm

for 115 V........................................................................................................T 0.4 A; 250 V; 5 x 20 mm

(6) Light source

Halogen lamp................................................................................................................... HAL 6 V 20 W

Adjustment of the light source...................................................................... continuous, 1.5 to 6 V DC

Color temperature at 6 V...........................................................................................................2800 K

Light flux..................................................................................................................................... 280 lm

Average life................................................................................................................................. 1000 h

Luminous area....................................................................................................................2.0 x 2.0 mm

(7) Opto-mechanical data

Stand with stage focusing........................................................................... with coarse drive (4mm/rot)

.....................................................................................................................and fine drive (0.4mm /rot)

...................................................................................................................................Overall lift 15 mm

Objective change.............................................................................................manually via 5x nosepiece

Objectives.................................................................................ICS line of objectives with W 0.8" thread

Eyepieces ........................................................................................................... 30 mm plug-in diameter

with field number 18.......................................................... Pl 10x/18 Br. and Pl 10x/18 Br. foc. or

with field number 20........................................................ E-Pl 10x/20 Br. and E-Pl 10x/20 Br. foc.

Specimen stage .............................................................. mechanical stage 75x30 R with ceramic surface

Dimensions (width x depth) ...................................................................................160 x 140 mm

Travel range (width x depth)......................................................................................75 x 30 mm

Specimen holder ...............................................with spring clip to the right or for one-handed operation

Swing-in condenser 0.9 Z..........................................for M

.................................................................................. for M

 < 10x swing out condenser front lens 0.9

Obj.

  10x swing in condenser front lens 0.9

Obj.

Binocular tube 45°/20 ICS

maximum field number............................................................................................................20

interpupillary distance............................................................ can be set between 55 and 75 mm

Viewing angle ........................................................................................................................45°

Viewing height ..................................................................................................approx. 440 mm

Visual port..............................................................................................................tube factor 1x

For photography using artificial light color reversal film for 3200 K, the conversion filter CB 3 (467852) produces the correct color temperature in the light path.

The scale on the coarse drive (0 to 400) permits the orienting measurement of the object thickness:

1 increment corresponds to approx. 5 µm

B 40-029 e 01/98 1-11

Binocular phototube 35°/20

maximum field number........................................................................................................... 20

interpupillary distance ........................................................... can be set between 55 and 75 mm

viewing angle.........................................................................................................................35°

viewing height ..................................................................................................approx. 430 mm

visual port..............................................................................................................tube factor 1x

camera/video port..................................................................................................tube factor 1x

camera/video port..............................................................................................interface 60 mm

switched via sliding prism...........................................................100% obs / 50% obs : 50% doc

Standard 25

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1-12 B 40-029 e 01/98

Standard 25

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START-UP

Contents

2 START-UP.......................................................................................................................2-3

2.1 Unpacking the instrument................................................................................................2-3

2.2 Attachment of binocular tube 45°/20 ICS or binocular phototube 35°/20 ICS....................2-3

2.3 Screwing-in of objectives .................................................................................................2-3

2.4 Insertion of eyepieces ......................................................................................................2-4

2.4.1 Insertion of eyepiece reticle..............................................................................................2-4

2.4.2 Compensation of ametropia when eyepiece reticles are used ...........................................2-5

2.5 Setting of interpupillary distance and viewing height ........................................................2-5

2.6 Attachment of specimen holder to mechanical stage 75x30 R...........................................2-6

2.7 Attachment of condensers...............................................................................................2-7

2.7.1 Attach condenser 0.9 Z....................................................................................................2-7

2.7.2 Attach condenser II Z 0.9 Ph1, 2, 3 and D (0.6/0.9)...........................................................2-7

2.8 Connecting the instrument to the line..............................................................................2-8

List of illustrations

Fig. 2-1 Attachment of binocular tubes.........................................................................................2-3

Fig. 2-2 Screwing-in of objectives .................................................................................................2-3

Fig. 2-3 Attachment of eyepieces .................................................................................................2-4

Fig. 2-4 Insertion of eyepiece reticle..............................................................................................2-4

Fig. 2-5 Setting the interpupillary distance and viewing height of the binocular tube .....................2-5

Fig. 2-6 Attachment of specimen holder to mechanical stage 75x30 R...........................................2-6

Fig. 2-7 Attachment of condenser 0.9 Z........................................................................................2-7

Fig. 2-8 Attachment of phase contrast condenser II Z 0.9 Ph 1, 2, 3 and D (0.6/0.9) ......................2-7

Fig. 2-9 Connecting the instrument to the line..............................................................................2-8

B 40-029 e 01/98 2-1

Standard 25

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2-2 B 40-029 e 01/98

Standard 25

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2 START-UP

The Standard 25

microscope, including

ICS

accessories, is delivered in standard packaging. We would recommend you to keep the packaging material so that the instrument can be stored for a longer period of time or returned to the manufacturer.

2.1 Unpacking the instrument



Remove the microscope from the transport case and place it on the worktable.

ICS

2.2 Attach binocular tube 45°/20

binocular phototube 35°/20



Loosen hexagonal screw (2-1/5) using the SW3 ball-headed screwdriver (2-1/4). Remove dust covers (2-1/1) from tube underside and the dovetail mount on the stand.



Hold the binocular tube (2-1/3) or the binocular phototube 2-1/2) in a slightly inclined position and attach it to the stand mount via the dovetail. Turn the binocular tube into the required observation position and tighten the hexagonal screw using the screwdriver.

Fig. 2-1 Attachment of binocular tubes

2.3 Screwing-in of objectives



Remove dust caps (2-2/2) according to the number of objectives and screw objectives (22/3) in nosepiece (2-2/1) clockwise one by one, starting with the lowest magnification.

The dust caps should remain on those nosepiece eyes which are not required.

Fig. 2-2 Screwing-in of objectives

B 40-029 e 01/98 2-3

Fig. 2-3 Insertion of eyepieces

Standard 25

2.4 Insertion of eyepieces



Remove both protection caps (2-3/1) from the binocular tube (2-3/2).



Insert the fixed eyepiece, e.g. Pl 10x/18 Br. (23/3) in the right tube and the focusing eyepiece Pl 10x/18 Br. foc. (2-3/4) in the left tube.

The focusing eyepiece is used to compensate for ametropia of the eyes.

ICS

Fig. 2-4 Insertion of eyepiece reticle

If eyepiece reticles are inserted into the unscrewed mount by the customer, attent ion must be paid to the labelling being visible the right way up after insertion.

2.4.1 Insertion of eyepiece reticle

The eyepieces Pl 10x/18 Br. foc. and E-Pl 10x/20 Br. foc. are intended for use with eyepiece reticles (see overview under 1.6).

The slight image shift caused by the additional path through the glass is taken into account on the diopter scale by the fact that the zero point position is indicated not by the white dot W (24/W) but by the red dot R (2-4/R).

The eyepiece reticles (2-4/1) have been adhered to screw-in mounts (2-4/2) to allow easy replacement.



To replace a reticle, unscrew the screw-on mount or the part containing the eyepiece reticle and replace it with the required one.

2-4 B 40-029 e 01/98

Standard 25

ICS

2.4.2 Compensation of ametropia when eyepiece reticles are used

The correct use of an eyepiece reticle requires two focusing eyepieces, e.g. Pl 10x/18 Br. foc., to enable compensation of ametropia.



Use the eyelens of the focusing eyepiece to focus on the line figure of the eyepiece reticle; focus on the edge of the field of view if no eyepiece reticle is used.



Focus on the microscope image of a specimen via the focusing drive by looking through the eyepiece with reticle.



When the image and the eypiece reticle are in focus in the above eyepiece, focus the image for the second eye via the focusing eyelens of the second eyepiece.

The position of the focusing drive on the stand must not be changed.

2.5 Setting of interpupillary distance

and viewing height



The eyepiece distance is matched to the individual interpupillary distance by s winging the eyepiece tubes symmetrically towards one another.



The viewing height can be increased (2-5/A) or reduced (2-5/B) by turning the entire binocular tube through 180°.

Fig. 2-5 Setting the interpupillary distance

and viewing height of the binocular tube

B 40-029 e 01/98 2-5

Standard 25

ICS

2.6 Attachment of specimen holder to mechanical stage 75x30 R

Fig.2-6 Attachment of specimen holder to

mechanical stage 75x30 R

The Standard 25

features the mechanical stage

ICS

75x30 R with ceramic coating (2-6/6). The specimen is precisely moved in x and y using

the user-friendly coaxial drive (2-6/4). The x/y gradation on the stage surface (2-6/5) with

the two vernier scales helps to relocate certain specimen spots.

The readily mounted specimen holder with spring clip R (453536) (2-6/1) is part of the standard configuration.

As an alternative, it is also possible to use

- the specimen holder with sprig clip R (473448)

(2-6/2) or

- the specimen holder for one-handed operation

(453548) (2-6/3).



Here, the microscope slide (2-6/7) is inserted into the specimen holder along the guiding edge, the spring clip is swung out and will automatically clamp the microscope slide in its stop position.



The specimen holders can be easily exchanged in the x-direction using the two fixation screws on the guiding rail. Loosen the two fixation screws, pull out specimen holder to the front and insert new specimen holder until it engages in the guiding slots. Tighten the two fixation screws again.

2-6 B 40-029 e 01/98

Standard 25

ICS

2.7 Attachment of condensers

2.7.1 Attach condenser 0.9 Z



Unscrew both centering screws (2-7/3) on the condenser carrier (2-7/2) until the condenser 0. 9 Z (2-7/9) can be easily inserted in the condenser carrier.



Insert condenser 0.9 Z (2-7/9) in the condenser carrier (2-7/2) in such a way that, firstly, the dovetail of the condenser is pressed against spring pin (2-7/1) in the condenser carrier and, secondly, the aperture diaphragm lever (2-7/8) can be conveniently operated from the front.



Tighten both centering screws (2-7/3) on the condenser carrier (2-7/2) until they engage in the dovetail and keep the condenser (2-7/9) in position.

The Ph 2 phase stop carrier (2-7/4) must be mounted as follows:



Screw locking screw (2-7/6) into the left drilled hole (2-7/7) on the underside of the condenser carrier. The locking screw keeps the Ph 2 phase stop carrier in its functional position.



Then screw Ph 2 phase stop carrier (2-7/ 4) into the drilled hole (2-7/5) on the underside of the condenser carrier.

2.7.2 Attach phase contrast condenser II Z 0,9 Ph 1, 2, 3 and D (0.6/0.9)

With the exception of the orientation in the condenser carrier (2-7/2), the phase contrast condenser II Z 0.9 is inserted in the Standard 25

ICS

in the same way as the above condenser 0.9 Z.



Insert condenser II Z 0.9 (2-8/1) in the condenser carrier (2-7/2) in such a way that knob (2-8/2) points to the observer.

Fig. 2-7 Attachment of condenser 0.9 Z

Fig. 2-8 Attachment of phase contrast

condenser II Z 0.9 Ph 1, 2, 3 and D (0.6/0.9)

B 40-029 e 01/98 2-7

Standard 25

2.8 Connecting the instrument to the line

Check whether the voltage indicated at the rear of the instrument complies with the line voltage! Do not connect the instrument to the line if the inst r ume nt voltage and the line voltage are not identical and make sure to inform the nearest CZ agency or CZ service agency.



Connect the line cable with connector (2-9/4) to the instrument socket (2-9/3) and connect the earthcontact plug (2-9/5) to the line.



Switch on the instrument via the on/off switch (2-9/2) on the instrument rear.



The green LED (2-9/1) lights up to indicate that the instrument is ready for operation (on/off switch in "I" position), and the integrated halogen lamp must also be on.

ICS

Fig. 2-9 Connecting the instrument to the line

2-8 B 40-029 e 01/98

Standard 25

ICS

OPERATION

Contents

3 OPERATION ...................................................................................................................3-3

3.1 Switch on the instrument.................................................................................................3-3

3.2 Setting of transmitted-light brightfield for KÖHLER illumination........................................3-4

3.3 Setting of transmitted-light phase contrast.......................................................................3-6

3.4 Setting of transmitted-light darkfield................................................................................3-8

3.5 Setting of transmitted light polarization contrast ..............................................................3-9

3.6 Measurement of lengths................................................................................................3-10

3.7 Attachment of photomicrography equipment.................................................................3-11

3.7.1 Attachment of SLR camera, e.g. CONTAX 167 MT .........................................................3-11

3.7.2 Attachment of MC 80 DX microscope camera (35 mm film cassette)..............................3-13

3.8 Attachment of adapters for video cameras.....................................................................3-14

3.9 Insertion of 8x drawing eyepiece....................................................................................3-16

List of illustrations

Fig. 3-1 Switch on the instrument.................................................................................................3-3

Fig. 3-2 Insertion of filters.............................................................................................................3-3

Fig 3-3 Setting of brightfield........................................................................................................3-4

Fig 3-4 Setting of interpupillary distance of binocular tube ...........................................................3-4

Fig 3-5 Setting of diaphragm images according to KÖHLER..........................................................3-5

Fig 3-6 Setting of phase contrast using condenser 0.9 Z ..............................................................3-6

Fig 3-7 Centering of phase stops in phase contrast ......................................................................3-7

Fig 3-8 Setting of phase contrast using Ph condenser II Z 0.9........................................................3-7

Fig 3-9 Setting of polarization contrast ........................................................................................3-9

Fig 3-10 Measurement of lengths................................................................................................3-10

Fig 3-11 Attachment of SLR camera, e.g. CONTAX 167 MT.........................................................3-11

Fig 3-12 Attachment of MC 80 DX microscope camera................................................................3-13

Fig 3-13 Insertion of 8x drawing eyepiece ...................................................................................3-16

B 40-029 e 01/98 3-1

Standard 25

ICS

3-2 B 40-029 e 01/98

Standard 25

ICS

3 OPERATION

3.1 Switch on the instrument



Switch on the instrument via the on/off switch (3-1/3) on the instrument rear. The green LED (3-1/2) must light up.



Set the required brightness via the "Brightness" control (3-1/1).

Depending on the application, place one or several of the following dia. 32 filters (3-2/ 1) on the dustprotection glass of the luminous-field diaphragm:



interference wide-band filter, green, 32x4, for contrast enhancement in b/w photography of stained sections and for phase contrast;



CB 3 conversion filter, 32x2, to generate the correct color temperature of 3200 K when artificial light color reversal film is used.



CB 3, 32x2, and CB 12, 32x2, conversion filters for use with daylight color film

The Standard 25

is supplied with factory-aligned illumination. The illumination need not be

ICS

adjusted even when the lamp is exchanged by the customers themselves.

Fig. 3-1 Switch on the instrument

Fig. 3-2 Insertion of filters

B 40-029 e 01/98 3-3

3.2 Setting of transmitted-light brightfield for KÖHLER illumination

As described in chapter 2, the microscope is ready for operation and switched on according to section 3.1.



If the Ph 2 phase stop carrier (3-3/4) is available on condenser 0.9 Z (3-3/6), it should be sw ung out of the optical beam path for microscopy in transmitted-light brightfield.

Furthermore, the following basic settings of the microscope are required:



First, place a high-contrast specimen w ith 0.17 mm cover slip on the mechanical stage 75x30 R (3-3/8).



Swing 10x objective into beam path via knurled ring (3-3/9) of the nosepiece.



Look throught the fixed eyepiece of the binocular tube (3-3/1) first and focus on the

Fig. 3-3 Setting of brightfield

object via the focusing drive (3-3/2). Then set the focus for the other eye by turning

the eyelens of the focusing eyepiece.



The eyepiece distance is matched to the individual interpupillary distance by swinging the eyepiece tubes symmetrically towards one another.

Set the interpupillary distance in such a way that you can see a sharply limited field of view with both eyes.



The viewing height can be increased (3-4/A) or reduced (3-4/B) by turning the entire binocular tube through 180°.

Standard 25

ICS

Fig. 3-4 Setting of interpupillary distance of

the binocular tube

3-4 B 40-029 e 01/98

Standard 25

ICS

The KÖHLER illumination principle requires the following set tings of the condenser, the luminous-field diaphragm and the aperture diaphragm:



Move condenser 0.9 Z (3-3/6) to the upper stop position via the condenser drive, swing condenser front lens 0.9 into the optical beam path and move aperture diaphragm in the center position via lever (3-3/5).



Set (reduce) diameter of the luminous-field diaphragm (3-3/3) unt il it is visible in the field of view ( 35/A). Focus the edge of the luminous-field diaphragm by slightly lowering the condenser (color-free edge) (3-5/B) and center the image of the luminous-field diaphragm using the two condenser centering screws (3-3/7) (3-5/C).

Open the luminous-field diaphragm (3-3/3) until it just disappears behind the edge of t he field of view (3-5/D).

Fig. 3-5 Setting of diaphragm images according to KÖHLER



Depending on which specimen is used, the image contrast can be adjusted via the aperture diaphragm (3-3/5). For specimens featuring normal contrast, the aperture diaphragm (3-3/5) should be set to approx. 2/3 of the diameter of the exit pupil of the objective.

This can be easily checked and set when the eyepiece is removed (3-5/E).



Finally, refocus the specimen via the coaxial fine drive (3-3/2).

Since field size and objective aperture change after every objective change, the setting of the luminous-field diaphragm and the aperture diaphragm must be repeated as described above.



For all objective magnifications V

10x, the condenser front lens 0.9 must be swung out of

obj

the beam path.

B 40-029 e 01/98 3-5

Standard 25

ICS

3.3 Setting of transmitted-light phase contrast

The phase contrast technique is mainly used to increase the image contrast of unstained specimens. As described in chapter 2, the microscope is ready for operation and swit ched on according to section

3.1. The microscope must be set for transmitted-light brightfield, as described in section 3.2. The further steps depend on whether phase contrast equipment A or B is used.

Optimum phase contrast requires a high level of cleanliness! Therefore, clean the front lens of the objective used, the visible condenser surfaces, the upper cover slip surface and the lower carrier plate surface of the specimen with particular care and carefully remove grease.

Equipment A



condenser 0.9 Z (3-6/9) and Ph 2 phase stop carrier (3-6/7)



phase contrast objectives labelled "Ph 2" (3-6/11), suitable also for transmitted-light brightfield

Settings:



Place unstained specimen on the mechanical stage:



Screw phase-contrast objective, e.g. 40x/ 0.65 Ph 2 (3-6/11), into nosepiece and swing it into t he beam path.



Remove condenser front lens 0.9 from the beam path via lever (3-6/10).



Open the luminous-field diaphragm (3-6/5) and the condenser aperture diaphragm (3-6/8).



Swing in phase stop carrier (3-6/7) with Ph 2 phase stop.



Set the required brightness.



Check whether phase stop centering complies with figure (3-7/B). For this, remove one eyepiece and replace it with the diopter or a centering telescope (3-6/3).



If required, center the phase stop via the two centering screws (3-6/6) using the SW 1.5

Fig. 3-6 Setting of phase contrast using

condenser 0.9 Z

screwdriver. The diopter or the centering telescope (3-6/3) must then be replaced with the eyepiece again.

3-6 B 40-029 e 01/98

Standard 25

ICS

Complete phase contrast is available only if the dark phase ring in the objective covers the bright phase stop in the condenser completely and precisely (also see Fig. 3-7/B).

To achieve this, the diopter or the centering telescope (3-6/3) must be inserted in tube (3-6/4) instead of an eyepiece. Focus on the phase ring by turning the eyelens (3-6/1) of the centering telescope via the knurled ring (3-6/2).

Equipment B



phase contrast condenser II Z 0.9 Ph1, 2, 3 (3-8/1)



phase contrast objectives labelled "Ph 2" (3-6/11), suitable also for transmitted-light brightfield

Fig. 3-7 Centering of phase stops in phase

contrast

Settings:



Insert phase contrast condenser II Z 0.9 Ph 1, 2, 3 (3-8/1) in the condenser carrier as described in section 2.



Screw phase contrast objective (3-6/11) into the nosepiece and swing it into the optical beam path.



Set the object in brightfield first by having the turret disk (3-8/5) click-stop in position "J".



Swing in the condenser front lens (3-8/2) via

lever (3-8/4).



Reduce luminous-field diaphragm (3-6/5), focus it using the condenser, center it and open it again (as described in section 3.2, page3-5).



Optimum image contrast in brightfield is

Fig. 3-8 Setting of phase contrast using Ph

condenser II Z 0.9

obtained by setting the aperture diaphragm via setting wheel (3- 8/3). The aperture diaphragm can be centered using knurled wheel (3-8/6) and fixed using lever (3-8/7) which can be clamped in position.



Swing phase stop Ph 1, 2 or 3 assigned to the phase contrast objective in t he beam path via turret disk (3-8/5).



Check centering of both phase stops using the centering telescope (3-6/3) in the tube, as described above.



If the phase rings are out of center, as shown in Fig. ( 3-7/A), use knurled wheel (3-8/6) to make the bright phase stops in the condenser exactly congruent with the dark phase ring in the object ive, as shown in Fig. (3-7/B).

B 40-029 e 01/98 3-7

Standard 25

ICS

3.4 Setting of transmitted-light darkfield

Darkfield is mainly used for small or m inute objects such as treponema, spir ochaeta, cilia and bacteria, but also for emulsions or unstained objects in watery solutions.

The special benefit of the darkfield technique is its natural, true rendition of the original colors (color fidelity).

The equipment required for darkfield always includes a condenser with central stop, the numerical aperture of which exceeds that of the objective used. For further details please see the following table.

Condenser with illumination aperture suitable objective apertures

Ph condenser II Z 0.9 in position Ph 3 0.44 Ph condenser II Z 0.9 in position D 0.6/0.9)

dry darkfield condenser 0.7 - 0.85



0.4

0.4 -0.55

0.4 - 0.6

on condenser holder Z dry darkfield condenser 0.8 - 0.95

0.6 - 0.75

on condenser holder Z Ultracondenser 1.2 - 1.4

0.75 - 1.0

on condenser holder Z

Higher objective magnifications or apertures require objectives with an integrated iris aperture. As described in chapter 2, the microscope is ready for operation and swit ched on according to section

3.1. The microscope must be set for transmitted-light brightfield, as described in section 3.2.

Settings:



Correct condenser height until image background appears in optimum darkness.



Checking the objective pupil reveals whether it is dark indeed; for this purpose, remove the eyepiece from the tube and view the objective exit pupil.



Setting of darkfield is made easier by performing precentration using an objective of a lower pow er. Since the luminous field is visible only where particles light up, while the final specimen, however, might be "empty" over large areas, we would recommend you to use a specimen for initial setting which features a homogeneous distribution of details, e.g. a blood smear.

Darkfield microscopy requires specimens and optical surfaces to be extremely clean. Finger prints and traces of grease in particular have negative effects, since they brighten the background of the field of view.

3-8 B 40-029 e 01/98

Standard 25

ICS

3.5 Setting of transmitted-light polarization contrast

As described in chapter 2, the microscope is ready for operation and swit ched on according to section

3.1.

Transmitted-light polarization contrast requires the following equipment:

- polarizer, oriented in EAST-WEST direction,

(453615) (3-9/3), mounted on swing-out carrier.

- analyzer (3-9/1), oriented in NORTH-SOUTH

direction, and lambda plate (3-9/2) are mounted on two separate sliders (453692) and integrated into the stand head. To enable the function to be performed, the sliders must be pushed in.

Settings:



First, set a suitable object in brightfield as described in section 3.2.



Swing the polarizer (3-9/3) in the beam path.



If only the analyzer slider (3-9/1) is pushed in, black-and-white polarization contrast is produced; the additional insertion of the lambda plate (3-9/2) makes the objects appear in colored polarization contrast.

The sliders containing the analyzer and the lambda plate must always be pushed in until stop to make them fully effective and to avoid the field if view to be cut off.

Retrofitting of the polarization equipment can be perform ed by the Zeiss service agency (see section 4.4).

Fig. 3-9 Setting of polarization contrast

B 40-029 e 01/98 3-9

Standard 25

ICS

3.6 Measurement of lengths

The measurement of lengths using the Standard 25 ICS requires the following, for example:



stage micrometer, positive 5 + 100/100 y D = 0.17 mm and



eyepiece crossline micrometer 10:100, d = 26 mm An overview of available stage micrometers and eyepiece reticles is given in chapter 1.6. Before the length measurement using the microscope can be perfor med, the micrometer or scale value

of the used objective / eyepiece reticle combination must be determined. This scale value is exactly that distance in the specimen which complies to one interval of the used eyepiece crossline micrometer.

For calibration, align the scales of the stage micrometer and the crossline micrometer parallel to each other by turning the eyepiece, and make the zero lines of both scales exactly congruent. If, for example, 99 increments (of 20 µm each) of the stage micrometer correspond to exactly 100 increments of the crossline micrometer, as in Fig. 3-10, the resulting scale value k’ for the used objective / eyepiece reticle combination (A-Plan 10x/0.25 and crossline micrometer 10:100) is

k' =

x 10 m = 9.9 m

100

The distance to be measured should be  5 mm in the intermediate eyepiece image in order to keep the influence of random measuring deviations as low as possible. Other measuring errors may occur if the eyepiece has not been inserted into the tube until stop.

After exchange of the stage micrometer for the specimen to be measured, the measuring distance of interest results from the number of increments of the eyepiece crossline micrometer (tenth estimated) multiplied by the scale value k’. Example:

L = 35.5 x 9.9 mm = 351.5 µm

Particularly large object structures can also be determined by using the vernier scale gradations (0.1 mm) on the mechanical stage. Here, it might be necessary to determine the distance to be measured through calculation from a combined x and y measurement (Pythagoras).

Fig. 3-10 Measurement of lengths

3-10 B 40-029 e 01/98

Standard 25

ICS

3.7 Attachment of photomicrography equipment

The Standard 25

microscope with camera tube can be changed from observation to photography via

ICS

a pushrod (3-11/7 or 3-12/8) (pushrod pulled out for photomicrography). Special adapters allow commercially available 35 mm SLR cameras and special microscope cameras (e.g. MC 80 attached to the camera port of the Standard 25

. For the use of the photomicrography equipment,

ICS

) to be

please observe the relevant separate manuals in addition to the information provided in this manual.

3.7.1 Attachment of SLR camera, e.g. CONTAX 167 MT



Screw T-2 adapter for the CONTAX bayonet (311/3) on the 2.5x connector for T2 (3-11/4) (456005).



Attach the camera housing (3-11/ 2) and fix the cable release (3-11/1), if required.



Loosen the three hexagonal screws (3-11/6), remove the dust cover (3-11/8) from the camera tube (3-11/5) and insert the premounted unit A in the camera tube.



Align the camera unit in the required position and tighten the three hexagonal screws (3-8/6).



Pull out pushrod (3-11/7) completely for photomicrography.



When artificial light color reversal film is used, the CB 3 conversion filter provides the correct color temperature of 3200 K. The filt er must be placed on the dust protection glass of the luminous-field diaphragm (3-2/5), as mentioned in section 3.1.



For daylight color reversal film, the CB 12 conversion filter must be used in addition to the CB 3 conversion filter.

If focusing is not to be made via the viewfinder of the camera, the component with the eyepiece reticle must be screwed in the eyepieces (also see section 1.6).

Fig. 3-11 Attachment of SLR camera, e.g.

CONTAX 167 MT

B 40-029 e 01/98 3-11

Various T2 adapters for SLR cameras are listed below:

T2 adapters to SLR cameras Cat. No.

T2 adapter for CONTAX (CONTAX bayonet) 416010 T2 adapter for OLYMPUS OM (OM bayonet) 416002 T2 adapter for MINOLTA (SR bayonet) 416003 T2 adapter for CANON (FD bayonet) 416004 T2 adapter for NIKON (F bayonet) 416009 T2 adapter for PENTAX (KA bayonet) 416011

For detailed information on SLR cameras please see ma nual G 42-406/II entitled "35 mm SLR cameras for microscopes and stereomicroscopes".

Standard 25

ICS

3-12 B 40-029 e 01/98

Standard 25

ICS

3.7.2 Attachment of MC 80 DX microscope camera (35 mm film cassette)



Insert adapter 60 for microscope camera (312/5) (456006) in camera tube (3-12/6) and fix it using three hexagonal screws (3-12/7).



Insert projection lens P 2.5x (3-12/4) in adapter 60 for microscope camera (3-12/5).



Attach MC 80

basic body (3-12/2) on

adapter 60 for microscope cameras until stop and fix it by clamping ring (3-12/3) anticlockwise.



Attach 35 mm film cassette Mot DX (3-12/1) to the basic body in such a way that the contact pins firmly engage in the relevant sockets.



Pull out pushrod (3-12/8) completely for photomicrography.



When artificial light color reversal film is used, the CB 3 conversion filter provides the correct color temperature of 3200 K. The filter must be placed on the dust protection glass of the luminous-field diaphragm (3-2/5), as mentioned in section 3.1.



For daylight color reversal film, the CB 12 conversion filter must also be used. The filter must be placed on the dust cover of the luminous-field diaphragm (3-2/5), as described in section 3.1.

For detailed information on the MC 80

, please see manual B 40-

036-e.

Fig. 3-12 Attachment of MC 80

camera

microscope

B 40-029 e 01/98 3-13

Standard 25

ICS

3.8 Attachment of adapters for video cameras

The following video adapters and video zoom adapters with 60 mm interface permit the attachment of one-chip b/w and color CCD cameras and three-chip color CCD cameras to the camera tube of the Standard 25

ICS

Tube Adapter

Cameras















 









456105



60 C 2/3“

1.0x

456119

60 C 1/3“ (3CCD)

0.5x





456115

60 ENG 2/3“

1.0x







456107

60 C 2/3“

0.63x

456108

60 C 1/3“

0.4x

456117

60 ENG 2/3“

0.8x







456106

60 C 1/2“

0.5x

456123

Zoom 60 C 2/3“

0.4x ... 2x

456121

Zoom 60 ENG 2/3“

0.4x ... 2x











































456124

Zoom 60 ENG 1/2“

0.5x ... 2.4x



456122

Zoom 60 ENG 1/2“

0.4x ... 2x

456118

60 ENG 1/2“

0.63x



3-14 B 40-029 e 01/98

Standard 25

ICS

The connecting piece 60 - 44 also allows video adapters with 44 mm interface to be used with the camera tube of the Standard 25 ICS with 60 mm interface.

Video adapters

(Cat.No.)



456140

Connecting piece 60 - 44

Microscopes with 60 mm interface and all video adapters for 44 mm interface.

Suitable for:: Comments

Connects video adapters for 44mm interface to microscopes with 60 mm interface.

Attachment of video cameras:



Loosen three hexagonal screws and remove dust cover from the camera tube of the Standard 25



Screw video adapter or video zoom adapter with C-mount thread into the video camera. Insert video adapter or video zoom adapter in ENG 2/2" or ENG ½“ bayonet of the video camera and

clamp it tight.



Insert premounted unit (video camera with video adapt er or video zoom adapter) in camera tube of the Standard 25

, align it and fix it using the three hexagonal screws.

ICS

ICS.



Insert eyepiece with photo reticle in the binocular tube and align photo reticle parallel to the camera.



Pull out pushrod on the binocular phototube to direct 50% of the light to the camera port.



Set the required zoom magnification factor via the wheel of the video zoom adapter.



If required, adjust image brightness on the monitor by changing the lamp brightness on the microscope stand.

The instructions of the camera manufacturer must also be observed when operating the video camera.

B 40-029 e 01/98 3-15

3.9 Insertion of 8x drawing eyepiece

Standard 25

ICS

The 8x drawing eyepiece (444126) is an accessory for microscopic drawing and can only be used in combination with the binocular phototube 35°/20

CS on the Standard 25

The eyepiece contains a

ICS.

beam splitter which allows the simultaneous observation of the microscope image and the drawing area.

As described in chapter 2, the microscope is ready for operation and switched on according to section 3.1.

The microscope must be set for transmitted-light brightfield, as described in section 3.2.

Settings:



Remove one eyepiece (3-13/1) and insert the 8x drawing eyepiece (3-13/3) in the binocular phototube 35°/20

(3-13/2) instead of it.

ICS

Swing 8x drawing eyepiece until the drawing area (DIN A4 notepad) lying in front of the stand appears symmetrically aligned also in the field of view.



Screw on the drawing eyepiece.



Illuminate the drawing area in such a way that

Fig. 3-13 Insertion of 8x drawing eyepiece

it can be seen in the same quality as the microscope image. If required, reduce the brightness of the microscope image via the brightness control (3-1/1).



A special drawing pencil is supplied to facilitate drawing.

35°/20 means a viewing angle of 35° and the maximum field number 20.

3-16 B 40-029 e 01/98

Standard 25

ICS

CARE, MAINTENANCE AND TROUBLESHOOTING

Contents

4 CARE, MAINTENANCE AND TROUBLESHOOTING........................................................4-3

4.1 Care and maintenance of the instrument .........................................................................4-3

4.2 Troubleshooting ..............................................................................................................4-4

4.2.1 Changing the fuses..........................................................................................................4-4

4.2.2 Changing the 6 V 20 W halogen lamp.............................................................................4-5

4.2.3 Troubleshooting table .....................................................................................................4-6

4.3 Table of spares, wearing parts and tools ........................................................................4-10

4.4 Requesting service.........................................................................................................4-11

List of illustrations

Fig. 4-1 Changing the fuses..........................................................................................................4-4

Fig. 4-2 Changing the 6 V 20 W halogen lamp .............................................................................4-5

B 40-029 e 01/98 4-1

Standard 25

ICS

4-2 B 40-029 e 01/98

Standard 25

ICS

4 CARE, MAINTENANCE AND TROUBLESHOOTING

4.1 Care and maintenance of the instrument

Maintenance of the Standard 25



Cover the instrument with the dust cover after every use.



Do not set up the instrument in a damp room, i.e. max. humidity  85%.



Remove dust from optical surfaces using a rubber blower or a natural hair brush. Use alcohol to

is limited to the following operations:

ICS

remove grease from brush, then dry the brush. Remove stubborn dirt and fingerprints using a dustfree cloth or leather cloth.



Remove stubborn dirt (e.g. fingerprints) from optical surfaces using commercially available optics cleaning cloths; if necessary, slightly moisten the cloths with petroleum ether. Clean the front lenses of the objectives using petroleum ether, but do not use alcohol.

When using the Standard 25



Store the Standard 25

in humid climatic zones, proceed as follows:

ICS

in bright, dry and well ventilated rooms with a humidity of less than 85%;

ICS

store particularly sensitive components and accessories, such as objectives and eyepieces, in a dry closet.



When the equipment is stored in closed cases for a longer period of time, t he growth of fungus can be avoided by including cloths soaked in fungicide in the cases.

The risk of growth of fungus on opto-mechanical instruments always exists in the following conditions:



relative humidity of more than 75% and temperatur es between +15° C and +35° C for more than three days.



installation in dark rooms without air ventilation, and



dust deposits and fingerprints on optical surfaces.

B 40-029 e 01/98 4-3

4.2 Troubleshooting

Troubleshooting is described in further detail using the following two examples:



changing the fuses, and



changing the 6 V / 20 W halogen lamp. Further measures are summarized under 4.2.3.

4.2.1 Changing the fuses

Standard 25

ICS

Fig. 4-1 Changing the fuses



Switch off the instrument via the on/off switch (4-1/1) and disconnect earth-contact plug (4-1/4) from

the line; if required, also disconnet flat plug (4-1/3) from the instrument connector (4-1/2).



Check line cable and instrument plugs (4-1/3 and 4) and exchange them, if required.



Press marked lock (4-1/5) in the direction of the arrow and remove fuse holder (4-1/6). Check fuse

inserts (4-1/7) for compliance with the line voltage and check whether resistance wires are OK.

for 230 V: T 0.2 A; 250 V

for 115 V: T 0.4 A; 250 V

Be absolutely sure that you replace defective fuse inserts. For the catalogue numbers of spare fuses

please see page 4-10 in section 4.3.

4-4 B 40-029 e 01/98

Standard 25

ICS

4.2.2 Changing the 6 V 20 W halogen lamp

The following procedure is required to exchange the lamp:



Switch off lamp supply via on/off switch (4-1/2) and allow lamp to cool down for approx. 15 minutes.



Disconnect the earth-contact plug (4-1/4) from the line and remove the flat plug (4-1/3) from the instrument connector (4-1/2).



Disconnect the instrument from the line and place it on the back to make the underside of the pyramid stand accessible.



Fold down cover (4-2/2) and pull out defective halogen lamp (4-2/1).



Use the protective film or a clean paper cloth to hold the new 6 V 20 W halogen lamp and insert both lamp pins carefully into the receptacles.

Do not touch the lamp bulb with your bare hands; if required, clean t he bulb with clean alcohol before switching it on for the first time in order to avoid dirt to burn in.

The Standard 25

is supplied with factory-aligned illuminat ion. The illumination need not be

ICS

Fig. 4-2 Changing the 6 V 20 W halogen lamp

adjusted even when the lamp is exchanged by the customers themselves.



Fold up cover again, turn the stand upside again and connect the stand to the line.

B 40-029 e 01/98 4-5

4.2.3 Troubleshooting table

Problem Cause Remedy

Standard 25

ICS

vignetting or unhomogeneous image brightness in the field of view

the field of view is not entirely visible

The pushrod on the camera tube is not in the correct position (observation/photography)

Nosepiece with objective not switched to stop position

Move the pushrod in the correct position (end position), see pages 3-11 and 3-13.

Switch nosepiece with objective in stop position

Condenser not set correctly. Set condenser correctly

(adjustment, centering), see pages 3-4, 3-5

Aperture diaphragm not set correctly.

Set aperture diaphragm correctly (centering, opening), see page 3-5.

Luminous-field diaphragm not set correctly.

Set luminous-field diaphragm correctly (centering, opening), see page 3-5.

Filter not inserted correctly in filter mount.

Insert filter correctly in filter mount, see page 3-3.

4-6 B 40-029 e 01/98

Standard 25

ICS

Problem Cause Remedy

Low resolving power and poor image contrast

Aperture diaphragm opening not set correctly.

Set aperture diaphragm opening in accordance with the 2/3 rule or depending on the specimen features, see page 3-5.

Condenser not focused correctly and condenser lens 0.9 not switched correctly.

Wrong cover slip thickness for

0.17 transmitted light objectives

Use of no or unspecified immersion oil with CZ immersion

Focus condenser and set condenser lens 0.9 correctly or switch it off, see pages 3-4, 3-5.

Use the correct 0.17 mm cover slips

Use CZ immersion oil 518 N, see page 4-10.

objectives Air bubbles in the immersion oil Apply oil again to remove the

bubbles.

Immersion oil at the front lens of a dry objective

Corr. Ring is not set to the correct cover slip thickness

Clean the front lens of the dry objective, see page 4-3.

Set the corr. ring to the correct thickness, see page 1-6.

Dirt or dust on the optical surfaces of objectives, eyepieces,

Clean the appropriate components, see page 4-3

condensers or filters

Image aberration Condenser not set correctly. Set condenser correctly, see pages

3-4, 3-5

Nosepiece not correctly switched

Correctly click-stop nosepiece

to stop position

Great focus differences after objective change

Specimen is not fixed on the mechanical stage

Focusing eyepieces are not set correctly.

Correctly set specimen in specimen holder and fix it.

Set focusing eyepieces to the appropriate ametropia, s. page 2-5

B 40-029 e 01/98 4-7

Problem Cause Remedy

Standard 25

ICS

Left and right fields of view cannot be combined into an

Interpupillary distance of the binocular tube is not set correctly.

image

Focusing eyepieces are not set correctly.

Eye-fatiguing microscopy Interpupillary distance of the

binocular tube is not set correctly. Focusing eyepieces are not set

correctly. Image brightness not acceptable Reduce lamp voltage or insert

Dirt or dust in the field of view Condenser not focused correctly

and condenser lens 0.9 not switched correctly.

Aperture diaphragm opening too small

Dirt or dust on the optical surfaces of objectives, eyepieces, condensers, filters or specimens

Set interpupillary distance correctly, see pages 2-5, 3-4

Set focusing eyepieces to the appropriate ametropia, s. page 2-5

Set correct interpupillary distance, see pages 2-5 and 3-4.

Set focusing eyepieces to the appropriate ametropia, s. page 2-5

conversion filter Focus condenser and set

condenser lens 0.9 correctly or switch it off, see pages 3-4, 3-5.

Set aperture diaphragm opening in accordance with the 2/3 rule or depending on the specimen features, see page 3-5.

Clean the optical surfaces of the appropriate components, see page 4-3

The 6 V 20 W halogen lamp does not function although the on/off switch is in "on"

Line cable not connected to the line.

Connect line cable to the line and make sure to check the instrument and line voltage, see page 2-6.

position

6 V 20 W halogen lamp not installed

Install 6 V 20 W halogen lamp, see page 4-5

6 V 20 W halogen lamp defective Exchange 6 V 20 W halogen lamp,

see page 4-5

The specified 6 V 20 W halogen lamp is not used.

Use the specified 6 V 20 W

halogen lamp, see page 4-10. Defective fuses Exchange fuses, see page 4-4. Electronics module possibly

defective

Have electronics module checked

by service and replaced, if required

(see page 4-11)

4-8 B 40-029 e 01/98

Standard 25

ICS

Problem Cause Remedy

The 6 V 20 W halogen lamp flickers, unstable brightness

End of average life of 6 V 20 W halogen lamp

Incorrectly installed or broken line cable

The pins of the 6 V 20 W halogen lamp are not correctly inserted into the receptacle

Replace 6 V 20 W halogen lamp, see page 4-5

Correctly connect line cable or replace it, see page 4-4

Corectly insert pins of 6 V 20 W halogen lamp in receptacle, see page 4-5

B 40-029 e 01/98 4-9

4.3 Table of spares, consumables and tools

Description Cat. No. Application

Standard 25

ICS

6 V 20 W long-life halogen lamp 380079 9690.000 for the integrated Standard 25

ICS

illumination SW 3 ball-headed screwdriver 000000 0069.551 to change the tubes Eyepiece eyecup 444801 0000.00 recommended for techniques with

a low brightness level in order to

suppress light reflection Dust cover for nosepiece

Dust cover for eyepiece tube Immersion oil 518 N ;

20 ml oiler 100 ml bottle 250 ml bottle 500 ml bottle

462981 0000.000 000000 0168.373

444950 0000.000 444952 0000.000 444953 0000.000 444954 0000.000

to cover unused instrument

openings

for applications using immersion oil

Cleaning paper, 300 sheet 462975 0000.000 to clean optical surfaces G-fuse inserts (5 x 20 mm) ;

for 230 V; T 0.2 A; 250 V for 115 V; T 0.4 A; 250 V

000000 0127.013 000000 0127.016

electrical overload protection for

the integrated power supply Light filters;

interference wide-band filter, green, d=32x4 Interference band filter, green 546, d=32x3 CB 12 conversion filter, d=32x2 CB 6 conversion filter, d=32x2 CB 3 conversion filter, d=32x2 3200-5500 K conversion filter, d=32x2 N 0.25 neutral-density filter; d=32x2 N 0.06 neutral-density filter; d=32x2

467803 467807 467850 9901 467851 467852 467847 467849 467848

to enhance the contrast in b/w

photography and phase contrast;

for color photography using

daylight color films and artificial

light color reversal films

for observation and b/w

photography with transmission

information in %

0.50 gray filter, d = 32x4

0.12 gray filter, d = 32x4

0.03 gray filter, d = 32x4 KG 1 heat-protection filter, d = 32x2 Reflection heat protection filter, d=32x2

Dust cover K Dust cover G (only in combination with

467840 467841 467842 467830 467832

459300 0000.000 459306 0000.000

for photography without color

distortion, with transmission

information in %

to protect sensitive specimens from

heat

to cover the instrument when it is

not used. binoclar phototube)

4-10 B 40-029 e 01/98

Standard 25

ICS

4.4 Requesting service

All repairs of mechanical, optical or electronic components inside the instrument and of the electrical components of the Standard 25

may only be performed by Carl Zeiss service staff or specially

ICS

authorized personnel.

To ensure the optimum setting and trouble-free function of your microscope even for a longer period of time, we would recommend you to conclude a service/maintenance contract with Carl Zeiss.

In the case of subsequent orders or when service is required, please get in touch with your local Zeiss agency.

B 40-029 e 01/98 4-11

Standard 25

ICS

4-12 B 40-029 e 01/98

Standard 25

ICS

ANNEX



List of abbreviations ..................................................................................................................... A-3



Physical and technical units........................................................................................................... A-4



Certification in accordance with DIN EN ISO 9001 / DIN EN 46001................................................. A-5



EC conformity declaration ............................................................................................................ A-7

B 40-029 e 01/98 A-1

Standard 25

ICS

A-2 B 40-029 e 01/98

Standard 25

ICS

List of abbreviations

AC Alternating C

urrent AS aspheric A-Plan achromatic objectives featuring improved image flatness (ICS line)

Br. suitable for eyeglass wearers CB Correction Blue (conversion filter)

CCD Charge C

oupled Device CP-Achromat achromatic objective (ICS line) CSA Canadian S

tandards Association

d diameter D darkfield, or cover slip thickness DC Direct C

urrent DIN Deutsches Institut für Normung (German standards association) doc documentation DX coding system for the storage of electronically legible information,

e.g. film speed

EG European Community EMV electromagnetic compatibility EN European standards ENG Electronic N

ews Gathering E-PL name of eyepiece type with aspheric lens and flat field of view EWG European Economic Community

FAA free working distance foc. focusing

HAL halogen lamp H brightfield

ICS Infinity C

olor-corrected System IEC International Electrotechnical Commission IP International Protection (protection type) ISO International Standard Organization

J iris diaphragm on Ph condenser II Z 0.9 KG 1 heat-protection glass from Schott L left (drive control on the left of the mechanical stage)

LED Light E

mitting Diode

LD Long Distance MC Microscope Camera N neutral-density filter

B 40-029 e 01/98 A-3

PCB polychlorinated biphenyl Ph phase contrast Pl flatfield

R right (control on the right of the mechanical stage)

Standard 25

ICS

SLR Single L

ens Reflex SK protection class SW wrench opening s/w black-and-white

T slow-blow (fuse type) TV television T2-Adapter standard adapter for 35 mm cameras

UL Underwriters L V

Obj

magnification of the objective

aboratories (US test authority)

VDE association of German electrotechnicians vis visual

W 0,8" Whitworth-type thread (inch thread) 0.8" Z centering

Physical and technical units

A ampere ° angular degree

°C Centigrade h hour

Hz Hertz K Kelvin

kg kilogram lm Lumen (light flux) mm millimeter U rotations V Volt W Watt

A-4 B 40-029 e 01/98

Zeiss Standard 25 ICS User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual