Nihon Kohden MEK-7300K Operators Manual

Automated Hematology Analyzer MEK-7300K

2013/12/19

OPERATOR’S MANUAL

Automated

Hematology Analyzer

MEK-7300K

First Edition: 30 Jul 2012

Fifth Edition: 09 Dec 2013

0614-904173D

Printed:

Automated

Hematology Analyzer

MEK-7300K

If you have any comments or suggestions
on this manual, please contact us at:

www.nihonkohden.com

0614-904173D

In order to use this product safely and fully understand all its functions, make sure to read this manual before using the
product.

Keep this manual near the instrument or in the reach of the operator and refer to it whenever the operation is unclear.

The contents of this manual are subject to change without notice.

Copyright Notice

The entire contents of this manual are copyrighted by Nihon Kohden. All rights are reserved. No part of this document
may be reproduced, stored, or transmitted in any form or by any means (electronic, mechanical, photocopied, recorded,
or otherwise) without the prior written permission of Nihon Kohden.

Trademark

The mark printed on the SD card that is used in this instrument is a trademark. The company name and model name are
trademarks and registered trademarks of each company.

Contents

GENERAL HANDLING PRECAUTIONS ............................................................................. i

WARRANTY POLICY ......................................................................................................... ii

RESPONSIBILITIES – PROFESSIONAL USERS .............................................................. ii

EMC RELATED CAUTION ..................................................................................................iii

Conventions Used in this Manual and Instrument ............................................................. iv

Warnings, Cautions and Notes ................................................................................ iv

Explanations of the Symbols in this Manual and Instrument .................................... v

Caution Labels on the Analyzer ...............................................................................vii

Text Conventions in this Manual .............................................................................. ix

How to Use This Manual ..................................................................................................... x

Manual Organization................................................................................................. x

Manual Construction ................................................................................................ xi

Safety ....................................................................................................................... xi

Section 1 General ...................................................................................1.1

Introduction ......................................................................................................................1.2

Measured Parameters ...........................................................................................1.2

Features ...........................................................................................................................1.4

Panel Description ............................................................................................................. 1.6

Front Panel ............................................................................................................1.6

Right Side Panel .................................................................................................... 1.7

Left Side Panel ...................................................................................................... 1.8

Top Panel ............................................................................................................... 1.8

Rear Panel .............................................................................................................1.9

Basic Operations ...........................................................................................................1.10

Screen Information .............................................................................................. 1.10

Using Touch Screen Keys ....................................................................................1.11

Reagent System ............................................................................................................ 1.12

Introduction .......................................................................................................... 1.12

Reagents ............................................................................................................. 1.12

Diluent ....................................................................................................... 1.12

Lysing Reagent .......................................................................................... 1.12

Detergent ...................................................................................................1.12

Reagent Storage ....................................................................................... 1.13

Handling Reagent ...................................................................................... 1.13

Background Count ..................................................................................... 1.13

Controls and Calibrator ........................................................................................ 1.13

Controls .....................................................................................................1.13

Calibrator ................................................................................................... 1.13

Consumables ................................................................................................................. 1.14

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Section 2 Preparation ............................................................................ 2.1

General ............................................................................................................................2.2

Environmental Requirements ........................................................................................... 2.3

Operator’s Manual MEK-7300K C.1

CONTENTS

Initial Preparation ............................................................................................................. 2.4

Inventory ................................................................................................................ 2.4

Standard Accessories ............................................................................................ 2.4

Unpacking ..............................................................................................................2.5

Waste Disposal Requirements .............................................................................. 2.5

Installation Flowchart .......................................................................................................2.6

Connecting an External Instrument to the Analyzer ........................................................2.7

Connecting a PC ...................................................................................................2.8

Disconnecting the USB Cable .....................................................................2.8

Connecting a Card Printer .....................................................................................2.8

Connecting a ZK-820V Hand-held Bar Code Reader (Option) .............................. 2.8

Connecting the Power Cord and Grounding the Analyzer .............................................2.10

Connecting the Power Cord ................................................................................. 2.10

Equipotential Grounding ......................................................................................2.10

Connecting Tubes and Installing Reagents .................................................................... 2.11

Materials Required ..............................................................................................2.11

Connecting Tubes ................................................................................................ 2.13

Diluent Tube ............................................................................................... 2.14

Detergent Tube .......................................................................................... 2.14

Lysing Reagent Tube ................................................................................. 2.16

Waste Fluid Tube ....................................................................................... 2.17

Turning the Laser Switch On .......................................................................................... 2.18

Loading Recording Paper in the WA-730V Printer Unit (Option) ...................................2.19

Turning Power On ..........................................................................................................2.20

Check Before Turning Power On .......................................................................... 2.20

Turning On the Power .......................................................................................... 2.20

Checking the Date and Time Settings .................................................................2.22

Cleaning the Analyzer..........................................................................................2.22

Check After Turning On the Power .......................................................................2.22

Optical Adjustment ..............................................................................................2.22

Checking Daily Accuracy ...............................................................................................2.23

Measuring Background Noise..............................................................................2.23

Measuring Background Noise in Closed Mode ......................................... 2.23

Measuring Background Noise in Open Mode ............................................ 2.25

Results ......................................................................................................2.26

Measuring Hematology Control ...........................................................................2.27

Preparing SD Card ........................................................................................................2.30

Safety Information about the SD Card ................................................................. 2.30

Handling and Storage ................................................................................ 2.30

Data Processing ........................................................................................2.30

Inserting and Removing the SD Card .................................................................. 2.31

Section 3 Principles of Operation ........................................................3.1

Operation Theory ............................................................................................................. 3.2

Electric Cell Counting ............................................................................................ 3.2

Counting Method .........................................................................................3.2

Red Blood Cell and Platelet Counting .........................................................3.3

Principle of Hydraulic Operation ............................................................................3.3

C.2 Operator’s Manual MEK-7300K

CONTENTS

Hemoglobin Measurement ....................................................................................3.4

Chemical Processing ................................................................................... 3.4

Spectrophotometric Measurement Method ................................................. 3.4

Principle of WBC Differential Operation ................................................................. 3.5

Measurement Results ...................................................................................................... 3.6

WBC Scattergrams ................................................................................................ 3.6

Histograms ............................................................................................................. 3.7

Vertical Axis Type for the Histogram ............................................................3.7

RBC (Red Blood Cell) Distribution Histogram .............................................3.8

PLT (Platelet) Distribution Histogram ........................................................... 3.8

Flags ...................................................................................................................... 3.9

Section 4 Performance Characteristics and Specifications .............. 4.1

Specifications ...................................................................................................................4.2

Measured Parameters, Ranges and Reproducibility to Specimen ........................4.2

Detection Method ..................................................................................................4.2

Standardization Analysis Method .......................................................................... 4.3

Dilution Ratio ......................................................................................................... 4.3

Counting Time ....................................................................................................... 4.3

Display ................................................................................................................... 4.3

Data Storage .........................................................................................................4.3

Environmental Conditions ...................................................................................... 4.3

Power Requirements .............................................................................................4.4

Dimensions and Weight ......................................................................................... 4.4

Electromagnetic Compatibility ............................................................................... 4.4

Safety ..................................................................................................................... 4.4

Bar Code Specifications ........................................................................................ 4.5

Transfer Format PC (V03-01) ...........................................................................................4.6

Transfer Example to a PC ...................................................................................... 4.6

Transfer Format PC (V03-02) ...........................................................................................4.8

Transfer Example to a PC ...................................................................................... 4.8

Transfer Format PC (V05-01) .........................................................................................4.10

Transfer Example to a PC .................................................................................... 4.10

ASTM Communication ................................................................................................... 4.13

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Section 5 Operating Instructions .........................................................5.1

Screen Configuration .......................................................................................................5.4

Flowchart of Screens ............................................................................................. 5.4

Ready Screen ........................................................................................................ 5.6

Work List Screen ................................................................................................... 5.6

Data Screen ...........................................................................................................5.7

Quality Control Screen ..........................................................................................5.8

Calibration Screen .................................................................................................5.8

Operation Screen ..................................................................................................5.9

System Screen ...................................................................................................... 5.9

Changing Settings .........................................................................................................5.10

General ................................................................................................................ 5.10

Operator’s Manual MEK-7300K C.3

CONTENTS

System Screen .................................................................................................... 5.10

Assigning Users and Passwords ...............................................................5.10

Doing Maintenance ...................................................................................5.16

Checking the Error Log .............................................................................. 5.22

Performing an Optical Adjustment ............................................................. 5.23

Settings Screen ................................................................................................... 5.34

Displaying Settings Screen. .......................................................................5.34

Labeling and Selecting Sample Types ....................................................... 5.35

Setting Normal Range Upper and Lower Limits ........................................5.38

Changing the Limits ................................................................................... 5.39

Selecting Units ..........................................................................................5.40

Operation Settings ..................................................................................... 5.42

Flag Settings .............................................................................................5.45

QC Settings Window ................................................................................. 5.49

Setting Reagent Management ................................................................... 5.50

Setting Auto Priming/Cleaning...................................................................5.55

Setting Date and Time ............................................................................... 5.57

Synchronizing the Date and Time ............................................................. 5.59

Changing Output Format ........................................................................... 5.60

Formatting SD Card ..................................................................................5.72

Changing Sound and LCD Settings ..........................................................5.73

Initializing the Analyzer .............................................................................. 5.74

Interference Substances ................................................................................................ 5.76

Measurement ................................................................................................................. 5.78

General ................................................................................................................ 5.78

Open and Closed Sampling Modes ........................................................... 5.78

Dilution Modes ........................................................................................... 5.78

Sample Types ............................................................................................ 5.79

Single and Double Counting ...................................................................... 5.79

ID Number .................................................................................................5.80

Alarm Display ............................................................................................ 5.80

Data Storage ............................................................................................. 5.80

Printing and Sending Data ........................................................................5.80

Advanced Count ........................................................................................ 5.81

Counting Special Case Samples ............................................................... 5.81

Assigning an ID to a Sample and Patient ............................................................5.81

Not Assigning an ID ................................................................................... 5.82

Using Bar Codes ....................................................................................... 5.83

Measurement in Closed Mode ............................................................................. 5.83

Preparing a Sample ................................................................................... 5.83

Measuring a Sample in Closed Mode .......................................................5.85

Measurement in Open Mode ...............................................................................5.89

Measuring a Venous Sample in Normal Dilution Mode .............................5.89

Measuring a Pre-Dilution Sample .............................................................. 5.92

Measuring a Venous Sample in WBC Low/High Dilution Mode ................. 5.96

Measuring a Sample in Capillary Mode ............................................................... 5.98

Preparation ................................................................................................ 5.98

Measurement ............................................................................................5.99

Auto Recount ..................................................................................................... 5.100

C.4 Operator’s Manual MEK-7300K

CONTENTS

Recount on Alarm .................................................................................... 5.100

Recount on PLT Count ............................................................................ 5.100

Work Lists ....................................................................................................................5.101

General .............................................................................................................. 5.101

Entering New Work List Data .............................................................................5.101

Receiving Work List Data .................................................................................. 5.104

Editing Work List Data .......................................................................................5.105

Searching for Work List Data ............................................................................. 5.106

Deleting Work List Data .....................................................................................5.108

To Delete One Data ................................................................................. 5.108

To Delete Several Consecutive Data ....................................................... 5.108

Measuring a Sample Using the Work List .......................................................... 5.110

Description of the Results Screen ...............................................................................5.111

Enlarging Measurement Results ............................................................. 5.111

Large Scattergram ................................................................................... 5.112

Large Histogram ...................................................................................... 5.112

Measuring Units ......................................................................................5.113

Alarm Display ....................................................................................................5.113

Printing and Sending Results ............................................................................ 5.114

Auto Printing/Sending after Measurement...............................................5.114

Printing by Pressing [Print] Key ............................................................... 5.114

Sending Data to PC ................................................................................. 5.114

Handling Data ..............................................................................................................5.115

General .............................................................................................................. 5.115

Displaying Saved Data ....................................................................................... 5.115

Editing the ID of Saved Data ............................................................................. 5.117

Searching for Saved Data .................................................................................. 5.118

Printing and Sending Saved Data ...................................................................... 5.124

Automatic Printing and Sending Data after Measurement ...................... 5.124

Printing and Sending Stored Data ........................................................... 5.124

RS-232C Data Transfer ...................................................................................... 5.128

Deleting Saved Data .......................................................................................... 5.129

Deleting One Data ................................................................................... 5.129

Deleting Multiple Data ............................................................................. 5.130

Deleting All Stored Data ..........................................................................5.131

Turning the Power Off ..................................................................................................5.132

Daily Shutdown .................................................................................................. 5.132

Checking List Before Turning the Power Off ............................................5.132

Turning the Power Off .............................................................................. 5.132

Check List After Turning Power Off .......................................................... 5.132

Check List Before Long Term Storage .....................................................5.133

Auto Cleaning and Priming when Operating All Night ............................. 5.133

Strong Cleaning ...........................................................................................................5.134

Draining the Measurement Baths and Sub Baths ........................................................ 5.136

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Operator’s Manual MEK-7300K C.5

CONTENTS

Section 6 Calibration Procedures ......................................................... 6.1

General ............................................................................................................................6.2

When to Calibrate .................................................................................................. 6.2

Note on the Calibration Procedure ........................................................................ 6.3

Calibration in Closed Mode .............................................................................................. 6.5

Calibration in Open, Pre-dilution and Capillary Modes ....................................................6.9

Displaying the Calibration Measurement Data List ........................................................ 6.12

Displaying and Printing Calibration Data History ...........................................................6.15

HGB/HCT Calibration with Human Blood ...................................................................... 6.17

Measurement with the Analyzer .......................................................................... 6.17

HGB Measurement with a Spectrophotometer .................................................... 6.17

HCT Measurement with a Microhematocrit Centrifuge ........................................ 6.18

Determining the HGB and HCT Calibration Coefficient ....................................... 6.18

Hemoglobin Conversion Table (g/dL ↔ %SAHLI) .......................................................... 6.20

Section 7 Operational Precautions and Limitations ...........................7.1

Environmental Requirements ........................................................................................... 7.2

Reagent Storage and Handling .......................................................................................7.3

Section 8 Hazards .................................................................................. 8.1

Overview .......................................................................................................................... 8.2

Warning Conventions ............................................................................................ 8.2

Signal Words ...............................................................................................8.2

Safety Icons .................................................................................................8.2

Hazard Information and Precautions ............................................................................... 8.3

General .................................................................................................................. 8.3

Biohazards .............................................................................................................8.3

Handling and Disposing of Biohazardous Material ................................................ 8.4

Chemical Hazards ................................................................................................. 8.4

Electrical Hazards .................................................................................................. 8.4

Physical and Mechanical Hazards ......................................................................... 8.5

Reference ........................................................................................................................ 8.6

Section 9 Service and Maintenance ..................................................... 9.1

Maintenance .................................................................................................................... 9.3

General .................................................................................................................. 9.3

Disposing of Waste ................................................................................................9.4

Disposing of the Analyzer ...................................................................................... 9.4

Periodic Replacement Parts .................................................................................. 9.5

Repair Parts Availability Policy ..............................................................................9.5

Internal Battery ................................................................................................................9.6

Changing Internal Battery ...................................................................................... 9.6

Setting after Battery Change ................................................................................. 9.6

Preventive Maintenance Schedule ................................................................................... 9.7

Maintenance Schedule ..........................................................................................9.7

Displaying the User Maintenance Screen .............................................................. 9.8

C.6 Operator’s Manual MEK-7300K

CONTENTS

Maintenance Check Sheet ............................................................................................. 9.11

Inside Panel Components .............................................................................................. 9.13

Daily Maintenance Procedures ...................................................................................... 9.14

Checking Reagents and Other Consumables ..................................................... 9.14

Checking the Appearance of the Analyzer .......................................................... 9.14

Cleaning the Surface of the Analyzer ........................................................ 9.15

Disinfecting the Surface of the Analyzer ....................................................9.15

Checking the Reagent Connection Tubes ............................................................ 9.16

Checking the Power Cord and Grounding Lead ..................................................9.16

Checking the External Instrument Connection .................................................... 9.16

Checking the Power On .......................................................................................9.16

Calibrating the Touch Screen..................................................................... 9.16

Checking the Date and Time ...............................................................................9.17

Clock Accuracy .......................................................................................... 9.17

Checking Daily Accuracy ..................................................................................... 9.17

Checking Measurement Baths and Sub Baths .................................................... 9.17

Checking Pump Tube ...........................................................................................9.17

Every 200 Counts Maintenance Procedures ................................................................. 9.18

Performing Strong Cleaning ................................................................................ 9.18

Weekly/Every 300 Counts Maintenance Procedures ..................................................... 9.19

Checking/Cleaning Filters .................................................................................... 9.19

Monthly/Every 1,000 Counts Maintenance Procedures ................................................. 9.20

Replacing Filters .................................................................................................. 9.20

Materials Required .................................................................................... 9.20

Procedure .................................................................................................. 9.20

Checking and Cleaning Measurement Baths, Sub Baths and MC Tray ............... 9.22

Materials Required .................................................................................... 9.22

Procedure .................................................................................................. 9.22

Checking, Cleaning and Replacing the Rinse Unit, Sampling Nozzles, Cap

Pierce Nozzle and Sample Cup ........................................................................... 9.27

Materials Required .................................................................................... 9.27

Procedure .................................................................................................. 9.27

Every Four Months/Every 3,000 Counts Maintenance Procedures ............................... 9.32

Checking, Cleaning and Replacing the Sampling Nozzles .................................. 9.32

Materials Required .................................................................................... 9.32

Procedure .................................................................................................. 9.32

Replacing Pump Tube .......................................................................................... 9.36

Materials Required .................................................................................... 9.36

Procedure .................................................................................................. 9.36

As-Required Maintenance Procedures .......................................................................... 9.40

Removing a Clog from the Aperture ....................................................................9.40

Cleaning Aperture Caps ...................................................................................... 9.42

Materials Required .................................................................................... 9.42

Procedures ................................................................................................9.42

Checking the Prime Function ..............................................................................9.47

Checking the Drain Function ............................................................................... 9.47

Checking the Cleaning Function .......................................................................... 9.47

Checking the Electrical Circuits ...........................................................................9.48

Checking the Background Noise ......................................................................... 9.49

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Operator’s Manual MEK-7300K C.7

CONTENTS

Checking the Flow Cell ........................................................................................ 9.50

Adjusting the Flow Cell .............................................................................. 9.51

Checking the Optical Sensitivity .......................................................................... 9.52

Checking the Optical Sensitivity (fine) ....................................................... 9.53

Checking the X-R ................................................................................................. 9.54

Checking the Current Coefficient ......................................................................... 9.55

Checking the Coefficient after Calibration ........................................................... 9.55

Checking the External Instruments ...................................................................... 9.56

Printers ......................................................................................................9.56

ZK-820V Hand-held Bar Code Reader ...................................................... 9.56

PC .............................................................................................................9.56

Decontamination Protocol ...................................................................................9.56

Procedure .................................................................................................. 9.57

Storing and Transporting the Analyzer ................................................................. 9.60

Preparing the Analyzer for Long Term Storage or Transport .....................9.60

Using the Analyzer after Storage ............................................................... 9.61

Preparing the Analyzer for Short Term Transport ...................................... 9.61

Operation Window ......................................................................................................... 9.62

Displaying the Operation Window ........................................................................9.62

Refilling All the Reagents ....................................................................................9.64

Refilling the Diluent .............................................................................................. 9.65

Refilling the Lysing Reagent ................................................................................ 9.66

Refilling the Detergent .........................................................................................9.67

Performing Strong Cleaning ................................................................................ 9.68

Cleaning the Flow Cell ......................................................................................... 9.69

Cleaning the Baths .............................................................................................. 9.70

Removing a Clog .................................................................................................9.71

Draining the Analyzer .......................................................................................... 9.72

Draining Baths .....................................................................................................9.74

Checking the Circuit ............................................................................................. 9.75

Checking Background Noise ............................................................................... 9.77

Initializing Settings .........................................................................................................9.79

Section 10 Messages and Troubleshooting ......................................... 10.1

Troubleshooting .............................................................................................................. 10.2

Introduction .......................................................................................................... 10.2

Troubleshooting Tips and Techniques .................................................................. 10.3

Obtaining Technical Assistance ........................................................................... 10.3

Message and Troubleshooting .......................................................................................10.4

General ................................................................................................................ 10.4

Alarm Window ...................................................................................................... 10.5

Alarm Messages ............................................................................................................10.6

Inaccurate Counting and Other Problems .................................................................... 10.11

Data and Symbol Display ............................................................................................. 10.13

C.8 Operator’s Manual MEK-7300K

CONTENTS

Section 11 Quality Control .................................................................... 11.1

General ..........................................................................................................................11.3

When to Run QC ................................................................................................. 11.3

QC Methods ........................................................................................................11.3

Control Material ................................................................................................... 11.3

Quality Control Procedures ............................................................................................ 11.4

Guidelines for Running Controls .......................................................................... 11.4

Control Material Guidelines ................................................................................. 11.4

X-R Program .................................................................................................................. 11.5

General ................................................................................................................ 11.5

Overview of X-R Program and Quality Control Procedure ........................11.5

Calculation of X and R ............................................................................... 11.5

Calculation of Upper and Lower Limits of X and R .................................... 11.6

X-R Management Graph ........................................................................... 11.7

X-R Graph Example .................................................................................. 11.7

How to Read the X-R Graph ...................................................................... 11.9

Data Outside the Limits .............................................................................11.9

Changing the Quality Control Settings ..............................................................11.10

Precautions for Hematology Control .................................................................. 11.12

Counting the Hematology Control .....................................................................11.12

Deleting Quality Control Measurement Data .....................................................11.16

Handling X-R Data ............................................................................................. 11.17

Displaying the X-R Data List (History) ..................................................... 11.17

Deleting X-R Data .............................................................................................. 11.18

Deleting X-R Data Individually ................................................................. 11.18

Deleting X-R Data Consecutively ............................................................11.19

Setting Vial to X-R History .................................................................................11.20

Displaying Vial Management Window ................................................................ 11.20

Displaying X-R Multi Trendgraph ....................................................................... 11.21

Handling X-R Graphs.........................................................................................11.22

Changing the X-R Limits .................................................................................... 11.23

Saving X-R History Data .................................................................................... 11.25

L & J Program (Levey and Jennings) ........................................................................... 11.27

General .............................................................................................................. 11.27

Overview of L & J Program and Quality Control Procedure ....................11.27

Calculation of Upper and Lower Limits .................................................... 11.28

L & J Management Graph ....................................................................... 11.28

How to Read the L & J Graph .................................................................. 11.28

Data Outside the Limits ...........................................................................11.28

Changing the Quality Control Settings ..............................................................11.29

Precautions for Hematology Control .................................................................. 11.29

Counting the Hematology Control .....................................................................11.29

Handling L & J Data ........................................................................................... 11.33

Displaying the L & J Data List (History) ................................................... 11.33

Deleting L & J Data ............................................................................................ 11.34

Deleting L & J Data Individually ............................................................... 11.34

Deleting L & J Data Consecutively ..........................................................11.35

Setting Vial to L & J History ...............................................................................11.36

Displaying Vial Management Screen ................................................................. 11.36

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Operator’s Manual MEK-7300K C.9

CONTENTS

Displaying L & J Multi Trendgraph ..................................................................... 11.37

Handling L & J Graphs.......................................................................................11.38

Saving L & J History Data .................................................................................. 11.39

XB (X Batch) Program ................................................................................................. 11.41

General .............................................................................................................. 11.41

Overview of XB ........................................................................................ 11.41

Calculation of XB ..................................................................................... 11.41

Handling XB Data ..............................................................................................11.43

Handling XB Graph ............................................................................................ 11.45

Setting XB Medium and Limit Values ................................................................. 11.46

Appendix A Parts and Accessories .....................................................A.A.1

Standard Accessories .................................................................................................. A.A.2

Options ........................................................................................................................ A.A.4

Consumables ............................................................................................................... A.A.6

Appendix B Bar Codes ..........................................................................A.B.1

Bar Codes for Using the ZK-820V Hand-held Bar Code Reader ................................. A.B.2

Using Bar Codes ............................................................................................... A.B.2

Changing the Settings ....................................................................................... A.B.3

Appendix C Printing Examples .............................................................A.C.1

Printing Examples ........................................................................................................ A.C.2

Appendix D Factory Default Settings ................................................... A.D.1

C.10 Operator’s Manual MEK-7300K

GENERAL HANDLING PRECAUTIONS

This device is intended for use only by qualified healthcare personnel.
Use only Nihon Kohden approved products with this device. Use of non-approved products or
in a non-approved manner may affect the performance specifications of the device.

Please read these precautions thoroughly before attempting to operate the instrument.

1. To safely and effectively use the instrument, its operation must be fully understood.

2. When installing or storing the instrument, take the following precautions:

(1) Avoid moisture or contact with water, extreme atmospheric pressure, excessive humidity and temperatures, poorly

ventilated areas, and dust, saline or sulfuric air.
(2) Place the instrument on an even, level oor. Avoid vibration and mechanical shock, even during transport.
(3) Avoid placing in an area where chemicals are stored or where there is danger of gas leakage.
(4) The power line source to be applied to the instrument must correspond in frequency and voltage to product

specications, and have sufcient current capacity.
(5) Choose a room where proper grounding is available.

3. Before Operation

(1) Check that the instrument is in proper operating order.
(2) Check that the instrument is grounded properly.
(3) Check that all cords are connected properly.
(4) Check that battery level is acceptable and battery condition is good when using battery-operated models.

4. During Operation

(1) The instrument must receive continual, careful attention.
(2) Turn power off when trouble is found on the instrument.

5. To Shutdown After Use

(1) Turn power off with all switches and keys returned to their original positions.
(2) Remove the cords gently; do not use force to remove them.
(3) Clean the instrument together with all accessories for their next use.

6. The instrument must receive expert, professional attention for maintenance and repairs. When the instrument is

not functioning properly, it should be clearly marked to avoid operation while it is out of order.

7. The instrument must not be altered or modied in any way.

8. Maintenance and Inspection

(1) The instrument and parts must undergo regular maintenance inspection.
(2) If stored for extended periods without being used, make sure prior to operation that the instrument is in proper

operating condition.

Operator’s Manual MEK-7300K i

WARRANTY POLICY

Nihon Kohden Corporation (NKC) shall warrant its products against all defects in materials and workmanship for one year
from the date of delivery. However, consumable materials such as recording paper, ink, stylus and battery are excluded
from the warranty.

NKC or its authorized agents will repair or replace any products which prove to be defective during the warranty period,
provided these products are used as prescribed by the operating instructions given in the operator’s and service manuals.

No other party is authorized to make any warranty or assume liability for NKC’s products. NKC will not recognize any
other warranty, either implied or in writing. In addition, service, technical modication or any other product change
performed by someone other than NKC or its authorized agents without prior consent of NKC may be cause for voiding
this warranty.

Defective products or parts must be returned to NKC or its authorized agents, along with an explanation of the failure.
Shipping costs must be pre-paid.

This warranty does not apply to products that have been modied, disassembled, reinstalled or repaired without Nihon
Kohden approval or which have been subjected to neglect or accident, damage due to accident, re, lightning, vandalism,
water or other casualty, improper installation or application, or on which the original identication marks have been
removed.

In the USA and Canada other warranty policies may apply.

RESPONSIBILITIES – PROFESSIONAL USERS

This instrument must be used by a professional user with a full knowledge of operating this instrument, only for the stated
intended use and according to the instructions for use. Instructions in the operator’s manual must be followed, especially
the following points.

• Storage and stability of reagents

• Handling of reagents

• Instrument installation

• Connection of all tubes to inlets and outlets

• Connection of all tubes to reagents and waste container

• Checking the amount of reagents and waste uid

• Calibration

• Quality control

• Maintaining and servicing

If deviating from the instructions, the professional user does it at the risk and liability of the laboratory and only after
validation by the laboratory. Nihon Kohden has no responsibility over such deviations.

ii Operator’s Manual MEK-7300K

EMC RELATED CAUTION

This equipment and/or system complies with the International Standard EN 61326-2-6 for electromagnetic

compatibility for electrical equipment and/or system for measurement, control and laboratory use. However,

an electromagnetic environment that exceeds the limits or levels stipulated in the EN 61326-2-6, can

cause harmful interference to the equipment and/or system or cause the equipment and/or system to fail

to perform its intended function or degrade its intended performance. Therefore, during the operation of the

equipment and/or system, if there is any undesired deviation from its intended operational performance,

you must avoid, identify and resolve the adverse electromagnetic effect before continuing to use the

equipment and/or system.

The following describes some common interference sources and remedial actions:

1. Strong electromagnetic interference from a nearby emitter source such as an authorized radio station

or cellular phone:

Install the equipment and/or system at another location if it is interfered with by an emitter source

such as an authorized radio station. Keep the emitter source such as cellular phone away from the

equipment and/or system.

2. Radio-frequency interference from other equipment through the AC power supply of the equipment and/

or system:

Identify the cause of this interference and if possible remove this interference source. If this is not

possible, use a different power supply.

3. Effect of direct or indirect electrostatic discharge:

Make sure all users and patients in contact with the equipment and/or system are free from direct or

indirect electrostatic energy before using it. A humid room can help lessen this problem.

4. Electromagnetic interference with any radio wave receiver such as radio or television:

If the equipment and/or system interferes with any radio wave receiver, locate the equipment and/or

system as far as possible from the radio wave receiver.

If the above suggested remedial actions do not solve the problem, consult your Nihon Kohden

representative for additional suggestions.

This equipment complies with International Standard EN 55011: 2007 Group 1, Class B. Class B EQUIPMENT is

equipment suitable for use in domestic establishments and in establishments directly connected to a low voltage

power supply network which supplies buildings used for domestic purposes.

The CE mark is a protected conformity mark of the European Community. Products with the CE mark comply

with the requirements of the In vitro Diagnostic Medical Device Directive 98/79/EC.

NOTE about Waste Electrical and Electronic Equipment (WEEE) directive 2002/96/EC

For the member states of the European Union only:

The purpose of WEEE directive 2002/96/EC is, as a first priority, the prevention of waste electrical and

electronic equipment (WEEE), and in addition, the reuse, recycling and other forms of recovery of such

wastes so as to reduce the disposal of waste.

Contact your Nihon Kohden representative for disposal.

Operator’s Manual MEK-7300K iii

Conventions Used in this Manual and Instrument

Warnings, Cautions and Notes

Warnings, cautions and notes are used in this manual to alert or signal the reader to specic information.

WARNING

A warning alerts the user to possible injury or death associated with the use or misuse of the instrument.

CAUTION

A caution alerts the user to possible injury or problems with the instrument associated with its use or

misuse such as instrument malfunction, instrument failure, damage to the instrument, or damage to other

property.

NOTE

A note provides specific information, in the form of recommendations, prerequirements, alternative methods or

supplemental information.

iv Operator’s Manual MEK-7300K

Explanations of the Symbols in this Manual and Instrument

The following symbols found in this manual/instrument bear the respective descriptions as given.

On panel

Symbol Description Symbol Description

AC power off
(Disconnection from the mains)

AC power on
(Connection to the mains)

Main power lamp Biohazard

“Off” only for part of the equipment Serial port 1

“On” only for part of the equipment Serial port 2

Auto print Serial port 3

Feed Printer socket

Print USB socket

Reset

Inlet

Outlet

Memory card socket
(Read data from store)

Clean Alternating current

Eject key (Tube holder open) Equipotential terminal

Count Fuse (time lag)

ISOTONAC•3
(diluent)

CLEANAC
(detergent)

HEMOLYNAC•3N
(hemolysing reagent)

HEMOLYNAC•5
(hemolysing reagent)

WASTE

The CE mark is a protected
conformity mark of the European
Community. Products marked
with this symbol comply with
the requirements of the In vitro
Diagnostic Medical Device Directive
98/79/EC.

IVD

Date of manufacture

Laser on

IN VITRO DIAGNOSTIC
MEDICAL DEVICE

Attention, consult operator’s manual

Products marked with this symbol
comply with the European WEEE
directive 2002/96/EC and require
separate waste collection. For Nihon
Kohden products marked with this
symbol, contact your Nihon Kohden
representative for disposal.

Operator’s Manual MEK-7300K v

On screen and recorded data

Symbol Description

When displayed beside NE, NE%, LY, LY%: Room temperature high
When displayed beside NE, NE%, LY, LY%, MO, MO%, EO, EO%, BA, BA%:
Optical count error
When displayed beside NE, NE%, LY, LY%, MO, MO%: Room temperature low
When displayed beside WBC or RBC measured value: Sample error
When displayed beside HGB measured value: Dirty measurement baths

When displayed beside WBC measured value: Poor hemolyzation
When displayed beside HGB measured value: HGB voltage adjustment error
When displayed beside MCHC measured value: Abnormal MCHC

When displayed beside WBC measured value: Small nucleated cell
When displayed beside NE, NE%, MO, MO%, BA, BA%: Immature granulocytes
When displayed beside NE, NE%, EO, EO%: Ne-Eo interference
When displayed beside NE, NE%, LY, LY%, MO, MO%, EO, EO%, BA, BA%:
Blasts
When displayed beside NE, NE%, EO, EO%: Left shift
When displayed beside LY, LY%, MO, MO%: Atypical lymphocytes
When displayed beside LY, LY%, MO, MO%: Ly-Mo interference
When displayed beside HGB measured value: HGB circuit error/WBC measured
value is OVER
When displayed beside RBC and PLT measured values: PLT-RBC interference
When displayed beside PLT: PLT low value (below 50,000/μL)

C

When displayed beside WBC or PLT measured value: Platelet coagulation

Reagent level graph

SD card is inserted

Open the alarm window

vi Operator’s Manual MEK-7300K

Caution Labels on the Analyzer

Refer to “Connecting Tubes” in Section 2.

Refer to warnings and cautions in
“Connecting the Power Cord and
Grounding the Analyzer” in Section 2.

Operator’s Manual MEK-7300K vii

NOTE

• Replace the filters periodically.

• When attaching the filter joint assembly, be careful not to bend or

damage the filter packing at the bottom of the measurement bath.

• When there is a leakage, check that there is no scratch or damage to

the circumference of the filter.

viii Operator’s Manual MEK-7300K

Text Conventions in this Manual

In this manual, procedural instructions are explained in logical groups, using numbered steps. Illustrations and drawings
appear where they are useful to the explanation. Text conventions are as follows:

Screen Name

The screen name is printed in mixed-case, regular letters; for example, Ready screen.

Touch Screen Keys

The screen has some touch screen keys which are pressure-sensitive. Pressing one of these touch screen keys initiates the
action specied by a screen label. Screen labels are shown in mixed-case, regular letters; for example, Quality control key.

Data Entry Field Names

Fields that accept data entered by the Operator have their names shown in regular, mixed-case font enclosed within carats < >.

Hard Keys (Keys on the Panels)

The keys on the panels are shown in regular, mixed-case font enclosed in brackets; for example, [Clean].

Screen Messages

Screen messages or other screen displays will appear in regular, mixed-case font enclosed in quotation marks, for example,
“Priming”.

Information Presentation Examples

Screen name Regular, Mixed-Case Menu screen
Touch screen keys Regular, Mixed-Case Quality control
Field names Regular, Mixed-Case, enclosed within carats < > <Dilute mode>
Hard keys (Panel keys) Regular, Mixed-Case, enclosed within brackets [Clean]
Screen Message Regular, Mixed-Case, enclosed within quotation marks “Priming”

Operator’s Manual MEK-7300K ix

How to Use This Manual

Manual Organization

The major sections of the manual and their contents are as follows:

Section 1: General

This section provides an overall description of the system. It names the major
system components and describes their uses or functions.

Section 2: Preparation

This section provides detailed instructions for system setup and conguration. It
explains proper location, requirements, and steps for installation.

Section 3: Principles of Operation

This section explains the principles behind the system’s operation. It describes
what the system measures and how those measurements are made.

Section 4: Performance Characteristics and Specifications

This section contains useful details on the dimensions of the analyzer, proper
operating environment, and performance specications.

Section 5: Operating Instructions

This section explains the procedures for daily start-up and shutdown, sample
collection and handling, and routine operation of the analyzer including use of
stored data.

Section 6: Calibration Procedures

This section describes the calibration process. It discusses calibration materials,
guidelines, and methods.

Section 7: Operational Precautions and Limitations

This section contains a summary of known factors that may adversely affect the
proper operation of the analyzer or the quality of the output.

Section 8: Hazards

This section covers possible hazards arising from the operation of the analyzer,
as well as decontamination and waste handling procedures.

Section 9: Service and Maintenance

This section discusses routine maintenance and cleaning on a daily, weekly,
monthly, and as required basis. Also included are detailed instructions for
removing and cleaning certain components to ensure proper system performance.

Section 10: Messages and Troubleshooting

This section contains a troubleshooting guide to help users identify probable
causes of a system malfunction or of suspect data, and to suggest the proper
corrective action.

x Operator’s Manual MEK-7300K

Section 11: Quality Control

This section covers the proper mixing, handling, and running of control material,
setting up QC les, and using the QC capabilities of the analyzer.

Appendices

Appendix A

This appendix lists the part numbers of components, accessories, controls,
reagents, and consumables associated with the analyzer for user convenience
when placing orders.

Appendix B

This appendix contains information on setup of the optional bar code reader.

Appendix C

This appendix contains specimen data reports.

Appendix D

This appendix contains the list of factory default settings.

Manual Construction

Safety

The physical construction of the manual supports its sectional organization.

Contents

The Contents at the beginning of this manual lists each section and its
subsections.

Section Separators

A large separator tab marks the start of each section.

Throughout the manual, signal words appear where the nature of the information
warrants special attention.

Operation, maintenance, and servicing of hematology systems may expose
individuals to potential safety and health hazards. All work must be performed as
described in the operator’s manual or as directed by Nihon Kohden. For detailed
safety information, refer to Section 8 “Hazards”.

Warnings are inserted throughout this manual to alert personnel to potential
hazards.

For detailed safety information, refer to Section 7 “Operational Precautions and
Limitations” and Section 8 “Hazards” in this manual.

Operator’s Manual MEK-7300K xi

Section 1 General

Introduction .......................................................................................................................................................... 1.2

Measured Parameters ............................................................................................................................... 1.2

Features ..............................................................................................................................................................1.4

Panel Description ................................................................................................................................................1.6

Front Panel ................................................................................................................................................ 1.6

Right Side Panel ........................................................................................................................................ 1.7

Left Side Panel ..........................................................................................................................................1.8

Top Panel...................................................................................................................................................1.8

Rear Panel ................................................................................................................................................1.9

Basic Operations ............................................................................................................................................... 1.10

Screen Information ..................................................................................................................................1.10

Using Touch Screen Keys ........................................................................................................................1.11

Reagent System ................................................................................................................................................1.12

Introduction .............................................................................................................................................. 1.12

Reagents .................................................................................................................................................1.12

Diluent ...........................................................................................................................................1.12

Lysing Reagent ............................................................................................................................. 1.12

Detergent ......................................................................................................................................1.12

Reagent Storage ...........................................................................................................................1.13

Handling Reagent .........................................................................................................................1.13

Background Count ........................................................................................................................1.13

Controls and Calibrator ...........................................................................................................................1.13

Controls ......................................................................................................................................... 1.13

Calibrator ....................................................................................................................................... 1.13

Consumables ..................................................................................................................................................... 1.14

1

Operator’s Manual MEK-7300K 1.1

1. GENERAL

Introduction

The MEK-7300K Automated Hematology Analyzer provides simultaneous 23
parameter and 2 research parameter measurement. It provides quick counting and
all operations are performed automatically just by putting the sampling nozzle
into a sample container with whole blood and pressing the [ Count] switch.
The analyzer also has a cap pierce unit to measure samples in capped tubes in
closed mode. Results and data are displayed on a color LCD screen and full
reports can be printed on an optional printer.

The analyzer automatically cleans blood from the sampling nozzle, so it is safe and
there is no risk of touching the blood. The analyzer has self-check, quality control
and other programs for reliable data management system.

To achieve full performance, thoroughly read this operator’s manual before
operating the analyzer.

Measured Parameters

To use the analyzer safely and effectively and keep it in optimum condition,
follow the operating and maintenance instructions in this manual.

NOTE

Use only Nihon Kohden parts and accessories to assure maximum
performance from your instrument.

23 parameters and 2 research parameters can be measured on the MEK-7300K
hematology analyzer.

WBC*1: White Blood Cell Count
NE%: Neutrophil Percent
LY%: Lymphocyte Percent
MO%: Monocyte Percent
EO%: Eosinophil Percent
BA%: Basophil Percent
NE: Neutrophil Count
LY: Lymphocyte Count
MO: Monocyte Count
EO: Eosinophil Count
BA: Basophil Count
RBC*1: Red Blood Cell Count
HGB*1: Hemoglobin Concentration
HCT*1: Hematocrit Percent

HCT =

Red blood cell volume

× 100

Blood volume

MCV*1: Mean Corpuscular Volume

MCV =

1.2 Operator’s Manual MEK-7300K

HCT (%)

× 10

RBC (× 106/µL)

1. GENERAL

MCH*1: Mean Corpuscular Hemoglobin

MCH =

MCHC*1: Mean Corpuscular Hemoglobin Concentration

MCHC =

RDW-CV: Red Blood Cell Distribution Width in CV
RDW-SD: Red Blood Cell Distribution Width in SD
PLT*1: Platelet Count
PCT: Platelet Crit
MPV: Mean Platelet Volume
PDW: Platelet Distribution Width
IG%*2: Immature Granulocyte Percent
IG*2: Immature Granulocyte

*1 CBC (complete blood count) parameters
*2 Research parameters

HGB (g/dL)

× 10

RBC (× 106/µL)

HGB (g/dL)

× 100

HCT (%)

1

Operator’s Manual MEK-7300K 1.3

1. GENERAL

Features

• Simultaneous 23 parameter measurement

The analyzer simultaneously measures 23 parameters (CBC and WBC 5 part

differential). WBC, RBC and PLT are measured by the electrical resistance
detection method. WBC is differentiated into neutrophil, lymphocyte,
monocyte, eosinophil and basophil by the light scatter technique - ow
cytometry with laser.

• Infection prevention

To help eliminate the risk of touching blood, you can perform measurement in

closed mode without removing the cap of the sample tube.

• Automatic sampling

Once the sample is aspirated through the sampling nozzle, all other operations

are performed automatically. The sample is automatically diluted and
measured. After measurement, the analyzer is cleaned and the waste uid is
automatically treated.

• Automatic sampling nozzle cleaning

The analyzer automatically cleans remaining blood from the sampling nozzle.

This also helps eliminate the risk of touching blood during measurement.

• Automatic clog removal and recounting

After each counting, the analyzer removes blood protein and dust particles

from around the aperture caps to prevent clogging. Even if a clog occurs, the
analyzer automatically removes the clog and recounts the sample.

• High accuracy and reproducibility

The analyzer reduces counting error by automatically diluting samples

and wiping the sampling nozzle. The analyzer provides high accuracy and
reproducibility with a low dilution ratio of 200:1 for WBC, 40,000:1 for RBC
and 360 µL of diluted sample. Built-in circuits automatically compensate
for cell miscount due to coincidence (simultaneous cell passage) and uid
temperature variation. The aperture is shielded from external noise and
clogging is prevented.

• Color LCD touch screen

The analyzer has a color TFT LCD with 800 × 600 pixel resolution which

clearly displays the results and various messages. The touch screen allows easy
and intuitive operation of the analyzer. You can enlarge the numerical data and
scattergram by touching them. Screen messages prompt the operator through
operation.

1.4 Operator’s Manual MEK-7300K

1. GENERAL

• Pre-diluted blood counting

Pre-diluted blood (10 or 20 µL) can be measured. The sample needs to be

diluted before measurement. 30 µL of venous blood can also be measured.
This sample does not need to be diluted before measurement.

• Automatic self-check

When the analyzer is turned on, the analyzer automatically starts priming and

checks itself. If a problem is detected, the LCD displays an alarm message, e.g.
BUBBLE, NO DILUENT or CLOG, so you can quickly identify and x the
problem.

• Variety of quality control programs

A variety of quality control programs are provided to enable calibration for

WBC, RBC, PLT, HGB and HCT, calculation of mean value and CV value, X­R and L & J quality control programs, and normal range setting on the screen.

• Data management

The analyzer can store measurement data of up to 400 samples and histograms

of up to 50 samples. Stored measurement data can be printed, deleted or
transferred to an external device.

1

• Automatic priming and cleaning

The analyzer automatically primes the uid path when the power is turned on

and cleans the uid path when the power is turned off.

• Selectable 23 parameters or 8 (CBC) parameters measurement

Measuring parameters can be selected from 8 (CBC) parameters or 23

(CBC and WBC 5 part differential) parameters. Measuring only the 8 CBC
parameters reduces reagent consumption.

• Password protected access

Measurement conditions, such as calibration coefcient, normal range and

quality control, are managed by the operator who has the password to access
these setting screens. Miscalculation caused by inappropriate measurement
condition settings can be avoided.

• High dilution measurement

When the WBC measured result is high, the sample can be recounted by

diluting the sample with higher ratio.

• Reagent management

The amount of reagent and waste uid can be monitored by the hematology

analyzer and a message can be displayed to alert the operator that a reagent is
nearly run out or the waste container is becoming full.

• Data sending and receiving with USB

The measurement data and work list can be sent and received by connecting a

personal computer to the USB connector (device) and using the QP-822V data
management software.

Operator’s Manual MEK-7300K 1.5

1. GENERAL

Panel Description

Front Panel

8

1

2

3
6
7
5

4

15

16

No. Name Description

1 Main power lamp Lights when the [Main power] switch on the rear panel is turned on.

2 Power lamp

3 Power key

4 Auto print key Switches the printing mode between automatic and manual for the printer.
5 Feed key Feeds paper of the printer while held down.
6 Print key Prints displayed data on the printer.

Auto print mode

7

lamp

8 LCD display Displays various messages, measured data and touch screen keys.

9 Reset key

10 Clean key

11 Eject key For closed mode only. Opens the tube holder to set the sample tube.

12 Tube holder

13 Sampling nozzle

14 Count switch For open mode only. Aspirates the sample and starts counting.

Printer unit

15

(WA-730VK)

16 Printer door

Lights when the [Main power] switch on the rear panel and [Power] key on the front
panel are turned on.

Turns the analyzer power on or off when the [Main power] switch on the rear panel
is turned on. When the power is turned on, priming and self-check are automatically
performed and the Ready screen appears.

Lights when automatic printing mode is selected.

Stops operation when pressed during operation. Returns to the Ready screen when
pressed while changing settings. Use this key only when an error occurs.

Cleans the uid path, aperture and manometer with detergent. Automatically primes
after cleaning the uid path. Press this key when clogging occurs, the manometer
becomes dirty or bubbles occur in the manometer.

For closed mode only. Holds a sealed vacuum blood collecting tube. Press the [Eject]
key to open. After measurement, the holder automatically opens.

For open mode only. Aspirates the sample. Dispenses the diluent when in the pre­dilution blood mode.

Thermal array printer. Prints out measured data and sample ID number (optional).

For the recording paper of the WA-730VK printer unit. To open, pull the upper left
corner (optional).

9

10

11

13

14
12

1.6 Operator’s Manual MEK-7300K

1. GENERAL

Right Side Panel

1

1 2 3 4 5 6 7

8

No. Name Description

1 Laser switch

ISO3

2

Diluent inlet
CLN

3

Detergent inlet
CLN3

4

Detergent inlet
HEMO3N

5

Lysing reagent inlet
HEMO5

6

Lysing reagent inlet
WASTE

7

Waste outlet

8 Vent hole for fan

Turns the laser on or off with the laser key for WBC 5 part
differential measurement.

Inlet for the ISOTONAC•3 diluent.

Inlet for the CLEANAC detergent.

Inlet for the CLEANAC•3 detergent.

Inlet for the Hemolynac•3N lysing reagent.

Inlet for the Hemolynac•5 lysing reagent.

Outlet for waste such as used lyse, detergent and aspirated
samples.

Vent hole for the fan.

NOTE

Do not block the hole. It affects the measurement

capability.

Operator’s Manual MEK-7300K 1.7

1. GENERAL

Left Side Panel

1

No. Name Description

Top Panel

1 SD card slot Insert an SD memory card.

1

No. Name Description

1 Flow cell cover For adjusting the ow cell position.

1.8 Operator’s Manual MEK-7300K

1. GENERAL

Rear Panel

No. Name Description

1 Main power switch

2 Fuse holder

3 Power socket Connects the AC power cord to supply AC power to the analyzer.

4

5 USB socket (device) Connects a personal computer to send and receive data.*
6 USB socket (host) Connects a barcode reader (Keyence BL-N60UB or equivalent).

7

8 Serial port 1 Connects to the optional WA-731V/461V card printer or PC.
9 Serial port 2 Connects to the optional WA-731V/461V card printer or PC.

10 Option port Connects to the external instrument.

4

1

2

3

Equipotential ground
terminal

ZK-820V
Bar code reader socket

1

5

6

7

8

9

10

Supplies the power to the analyzer when it is turned on. Under
normal conditions keep this switch turned on.

Contains the time lag fuse. To replace the fuse, contact your
Nihon Kohden representative.

Connects the ground lead to the equipotential ground terminal on
the wall for earth grounding.

Connects to an optional ZK-820V hand-held bar code reader and
supplies power to the bar code reader when connected.
Power supply voltage: 5 V DC (pin 9: 5 V, pin 5: GND)
Rated current: 200 mA

* To connect to a personal computer, the QP-822V data management software is required.

Operator’s Manual MEK-7300K 1.9

1. GENERAL

Basic Operations

Screen Information

Status bar

When the type of operator is “service”, the status bar is orange.
When the type of operator is “lab technician”, the status bar is blue.
When the type of operator is “other user”, the status bar is green.
For details, refer to “Assigning Users and Passwords” in Section 5.

Screen name

Check box

Date and time

For details, refer to
“Setting Date and Time”
in Section 5.

1.10 Operator’s Manual MEK-7300K

1. GENERAL

Using Touch Screen Keys

Do not use a sharp object to press the touch screen. Use your finger.

Cursor (blue)

NOTE

Displays the selection list

1

Selection list

Use arrow keys to move cursor Use the numeric keys to enter a value and

press the Enter key to register the setting

Displays measure
mode selection list

Displays sample type
selection list

Displays another screen

Operator’s Manual MEK-7300K 1.11

1. GENERAL

Reagent System

Introduction

A reagent system which is specically formulated for this analyzer provides
optimal system performance. Use of reagents other than those specied in this
manual is not recommended, as analyzer performance can be affected. Each
analyzer is tested at the factory using the specied reagents, and all performance
claims are generated using these reagents. For information on ordering reagents,
refer to Appendix A “Parts and Accessories”.

CAUTION

If reagent has been frozen, it must not be used.

Reagents

Diluent

Diluent is for doing the following:

• Act as the diluent for the WBCs, RBCs, PLTs, and HGB.

• Maintain the cell volume of each RBC and PLT during the count and sizing
portion of the measurement cycle.

• Provide a conductive medium for impedance counting and sizing of cells and
platelets.

• Rinse the sampling nozzles and ow systems.

Lysing Reagent

The analyzer uses lysing reagent as hemolysing reagents:

• Rapidly lyse the RBC and minimize the resultant cell stroma.

• Alter the WBC membrane to allow the cytoplasm to slowly diffuse and shrink
the membrane around the nucleus and any granules that may be present.

• Convert hemoglobin to a modied hemoglobin complex that is measurable at
540 nm (The quaternary ammonium lysate participates to form a chromogen
for hemoglobin measurement.).

Detergent

The analyzer can use two types of detergents: CLEANAC and CLEANAC•3:

• Provide an optically clear solution that is used to obtain the zero reference
during the HGB measurement cycle.

• Provide proper meniscus formation in both metering tubes and maintain it
during each run cycle.

• Rinse both measurement baths and sub baths, both metering tubes, and the
uid path with minimal bubble formation.

• Remove protein buildup and provide detergent cleaning within the analyzer. It
is used in scheduled and unscheduled maintenance.

1.12 Operator’s Manual MEK-7300K

1. GENERAL

Reagent Storage

Reagents must be stored at room temperature to ensure optimal performance.
All reagents should be protected from direct sunlight, extreme heat, and freezing
during storage. Temperatures lower than 0ºC (32ºF) can cause reagent layering
that changes the tonicity and conductivity of the reagents. If any reagent has been
frozen, it must be disposed of according to federal, state, and local regulations.

Each length of reagent inlet tubing is attached to a cap that minimizes
evaporation and contamination during use. Ensure that all reagent caps are not
damaged, and are securely attached to containers during use. Reagent quality can
deteriorate with time. Therefore, use all reagents before the expiration date on
the label.

Handling Reagent

When handling reagents, pay special attention to the following:

• Wear protective gloves when handling reagents.

• Never transfer the contents of a reagent container to an unmarked container or
other reagent container.

• Thoroughly clean all spills. Remove any dried residue in and around the
reagent inlet connectors located on the right side panel of the analyzer.

• Dispose of reagents and waste uids according to federal, state, and local
regulations.

• Always wash your hands after handling reagents.

1

Controls and Calibrator

Background Count

Always run a background count after installing a fresh container of reagent.
Values reported must be within the following specications:

• WBC ≤ 0.2 (×103/μL)

• RBC ≤ 0.05 (×106/μL)

• HGB ≤ 0.1 g/dL

• PLT ≤ 10 (×103/μL)

Controls and calibrator are reference materials used to test, set, and monitor
analyzer performance. For information on ordering controls and calibrators, refer
to Appendix A “Parts and Accessories”.

Controls

Day-to-day verication of system calibration is performed using controls.
Running these stabilized reference products is recommended to test analyzer
accuracy. The following controls are used in the analyzer system:

Control: tri-level whole blood quality control materials designed to monitor CBC
and WBC values obtained on hematology systems.

Calibrator

Calibration of the directly-measured parameters can be performed using
calibrator. Calibration is discussed in Section 6 “Calibration Procedures”.

Operator’s Manual MEK-7300K 1.13

1. GENERAL

Consumables

For information on ordering parts, accessories, reagents, controls, calibrators,
and consumables, refer to Appendix A “Parts and Accessories”.

1.14 Operator’s Manual MEK-7300K

Section 2 Preparation

General ................................................................................................................................................................ 2.2

Environmental Requirements .............................................................................................................................. 2.3

Initial Preparation ................................................................................................................................................. 2.4

Inventory .................................................................................................................................................... 2.4

Standard Accessories ...............................................................................................................................2.4

Unpacking .................................................................................................................................................2.5

Waste Disposal Requirements ..................................................................................................................2.5

Installation Flowchart ........................................................................................................................................... 2.6

Connecting an External Instrument to the Analyzer ............................................................................................ 2.7

Connecting a PC ....................................................................................................................................... 2.8

Disconnecting the USB Cable ......................................................................................................... 2.8

Connecting a Card Printer ......................................................................................................................... 2.8

Connecting a ZK-820V Hand-held Bar Code Reader (Option) .................................................................2.8

Connecting the Power Cord and Grounding the Analyzer .................................................................................2.10

Connecting the Power Cord .................................................................................................................... 2.10

Equipotential Grounding .......................................................................................................................... 2.10

Connecting Tubes and Installing Reagents ....................................................................................................... 2.11

Materials Required .................................................................................................................................. 2.11

Connecting Tubes ....................................................................................................................................2.13

Diluent Tube .................................................................................................................................. 2.14

Detergent Tube ..............................................................................................................................2.14

Lysing Reagent Tube ..................................................................................................................... 2.16

Waste Fluid Tube ........................................................................................................................... 2.17

Turning the Laser Switch On .............................................................................................................................2.18

Loading Recording Paper in the WA-730V Printer Unit (Option) .......................................................................2.19

Turning Power On .............................................................................................................................................. 2.20

Check Before Turning Power On ............................................................................................................. 2.20

Turning On the Power ..............................................................................................................................2.20

Checking the Date and Time Settings ..................................................................................................... 2.22

Cleaning the Analyzer ............................................................................................................................. 2.22

Check After Turning On the Power .......................................................................................................... 2.22

Optical Adjustment .................................................................................................................................. 2.22

Checking Daily Accuracy ................................................................................................................................... 2.23

Measuring Background Noise ................................................................................................................. 2.23

Measuring Background Noise in Closed Mode .............................................................................2.23

Measuring Background Noise in Open Mode ...............................................................................2.25

Results .......................................................................................................................................... 2.26

Measuring Hematology Control ............................................................................................................... 2.27

Preparing SD Card ............................................................................................................................................ 2.30

Safety Information about the SD Card ....................................................................................................2.30

Handling and Storage ...................................................................................................................2.30

Data Processing ............................................................................................................................ 2.30

Inserting and Removing the SD Card ..................................................................................................... 2.31

2

Operator’s Manual MEK-7300K 2.1

2. PREPARATION

General

WARNING

Never use the analyzer in the presence of any flammable anesthetic

gas or high concentration oxygen atmosphere. Failure to follow this

warning may cause explosion or fire.

WARNING

Never use the analyzer in a hyperbaric oxygen chamber. Failure to

follow this warning may cause explosion or fire.

2.2 Operator’s Manual MEK-7300K

Environmental Requirements

Install the analyzer outside the patient environment. If it is installed

inside the patient environment, the patient or operator may receive

electrical shock.

Use this analyzer under the following conditions.

Temperature: 15 to 30°C (59 to 86°F)

Humidity: 30 to 85% (noncondensing)

Air pressure: 70 to 106 kPa

70 kPa air pressure equals 3,000 m above sea level. Do not use the

analyzer at altitudes higher than 3,000 m above sea level.

2. PREPARATION

2

WARNING

CAUTION

15 cm

Left side

Rear

5 cm

Right side

Do not block
the vent hole
for the fan.

• Operate the analyzer in a room with a temperature range of 15 to 30°C (59 to
86°F). Keep the temperature of diluent and lysing reagent within this
temperature in order to obtain reliable data.

• No measurement can be done in dusty areas because the aperture for specimen
aspiration is very ne and can get clogged. Therefore, install the analyzer in a
dust-free area.

• Do not install the analyzer in direct sunlight.

• Do not place containers of reagent or uid on the analyzer. To prevent
electrical problems or electric shock, avoid spillage in or around the analyzer
because the uid is highly conductive.

• Select a stable, at buffering stand to set the analyzer on.

• If possible, use an independent AC outlet only for this analyzer. The analyzer
must not share an AC outlet with noise generating equipment such as a
centrifuge, constant temperature bath (thermostat), refrigerator, air conditioner
or ultrasonic cleaner.

• Make sure that there is more than 5 cm of space between the rear panel and
the wall and 15 cm of space between the left panel and the wall for adequate
ventilation.

Vent
hole
for fan

• Do not block the vent hole for the fan.

• When there is any problem in the analyzer, turn off the main power
immediately and disconnect the power cord from the AC outlet. Take the
analyzer out of service and check for damage.

Operator’s Manual MEK-7300K 2.3

2. PREPARATION

Initial Preparation

Inventory

Standard Accessories

Conrm that the analyzer shipment contains the following:

• Celltac Es hematology analyzer

• Standard accessories

• Reagents

• Controls and calibrator

• Operator’s manual

• Printer (optional)

• ZK-820V Hand-held bar code reader (optional)

• Power cord

• Ground lead

• Fuse, 3.15 A time-lag (2)

• Filter assy (4)

• Pump tube (N) assy (2)

• Sampling nozzle

• Diluent tube, marked blue, 1.5 m

• Waste tube, marked red, 1.5 m

• Detergent tube for CLEANAC, marked green, 1.5 m

• Cleanac tube 8 for CLEANAC•3, marked white, 1.5 m

• 18 L cap (3)

• 18 L tube assy 2

• 1 L tube assy

• Hemolynac•3 cap (yellow)

• Hemolynac•3 tube (570), marked yellow

• 18 L tube assy (waste)

• Hemolynac•5 cap (black)

• Hemolynac•5 tube (570), marked black

• Waste container (10 L)

• Cleanac tube assy (8222) (2)

• Laser key (2)

• T-wrench TBS-8 (8 mm)

Visually inspect these items for damage. If there is any damage, contact your
Nihon Kohden representative.

2.4 Operator’s Manual MEK-7300K

Unpacking

2. PREPARATION

Waste Disposal Requirements

Remove the analyzer from the shipping container and visually inspect for
damage. If there is any damage, contact your Nihon Kohden representative. Use
two people when lifting or moving the analyzer.

CAUTION

Use two people and be careful when moving the analyzer.

Otherwise, you may injure your back or be injured from dropping the

analyzer.

WARNING

Potential Biohazard. Observe all biosafety and chemical hazard

precautions for waste disposal. Operators are responsible for

disposing of waste in accordance with local, state, and federal

regulations.

2

CAUTION

Waste container must be stored at the same level or below the

analyzer, never above.

CAUTION

The waste is under pressure. Be sure that the waste tube is securely

placed in the waste container, flow of waste is unobstructed, and all

analyzer components are located away from possible waste overflow.

Operator’s Manual MEK-7300K 2.5

2. PREPARATION

Installation Flowchart

1. Place the analyzer in the appropriate place.

2. Connect external instruments such as printer, ZK-820V hand-held bar code
reader and PC, if necessary.

3. Connect the power cord and if necessary, perform grounding. Do not turn on
the power of any instrument at this stage.

4. Connect the diluent, detergent, lysing reagent and waste container to the
analyzer.

5. Check that the pump tubes are not disconnected or damaged.

6. Turn on the power of the analyzer and connected external instruments.

7. Perform Strong cleaning on the Operation screen.

8. Check the settings on the Settings screen. Make sure that the date and time
settings are correct. Refer to Section 5.

9. Calibrate the analyzer. Refer to Section 6.

10. Check the daily accuracy.

2.6 Operator’s Manual MEK-7300K

Connecting an External Instrument to the Analyzer

A personal computer, card printer (WA-460V/461V or equivalent) and external
printer can be connected to the serial port and a ZK-820V hand-held bar code
reader can be connected to the bar code reader socket on the rear panel.

Before connecting the external instrument to the analyzer, make sure that the
power on the instruments is turned off.

For changing the printing and communication format, refer to “Changing Output
Format” in Section 5.

CAUTION

When several medical instruments are used together, ground all

instruments to the same one-point ground. Any potential difference

between instruments may cause electrical shock to the operator.

2. PREPARATION

2

When more than one electrical instrument is used, there may be electrical
potential difference between the instruments. Potential difference between
instruments may cause current to ow to the connected to the instruments,
resulting in electrical shock.

Always perform equipotential grounding when required.

CAUTION

Connect only the specified instrument to the

analyzer and follow the specified procedure.

Failure to follow this instruction may cause

instrument malfunction.

CAUTION

The analyzer should only be connected to an

external instrument which complies with the

CISPR 11 (Edition 4: 2003), Group 1 and Class B

standard.

CAUTION

In order to avoid any safety hazard, only connect

personal computers which are approved by UL

60950.

CAUTION

Before connecting or disconnecting instruments,

make sure that each instrument is turned off and

the power cord is disconnected from the AC

socket. Otherwise, the operator may receive

electrical shock or injury.

Operator’s Manual MEK-7300K 2.7

2. PREPARATION

Bar code socket

Connecting a PC

USB socket
(device)

Serial ports

A locally purchased PC can be connected to the serial port or USB socket
(device) on the rear panel.

NOTE

•

In order to avoid any safety hazard, only connect personal computers

which are approved by UL 60950.

•

Only use the 3-prong power cord for the PC.

• Set the correct connection settings.

• The PC can be connected to either the serial port or USB socket. When

the PC is connected to the serial port, only the numeric data can be

sent to the PC; the scattergram and histogram cannot be sent.

• To connect the PC to the USB socket (device), the optional QP-822V

data management software is required. The measurement result can be

sent to the PC after every measurement.

Disconnecting the USB Cable

Disconnect the USB cable from the PC to disconnect the USB communication.
Connect the USB cable again to restart the communication. You cannot
disconnect the communication by clicking the icon on the task bar of the PC.

Connecting a Card Printer

The WA-460V/461V card printer can be connected to the serial port on the
analyzer. On the WA-460V/461V card printer, only the numeric data is printed.
Histograms cannot be printed.

Connect the card printer cable to the card printer and the serial port on the

Serial ports

analyzer. For changing the printing and communication format, refer to
“Changing Output Format” in Section 5.

Connecting a ZK-820V Hand-held Bar Code Reader (Option)

The optional ZK-820V hand-held bar code reader can be used for reading the bar
code label (up to 13 characters) on the sample tube. For details about the hand­held bar code reader, refer to the bar code reader manual.

The bar code reader can read the following codes:

• Industrial 2 of 5

• ITF

• JAN/EAN/UPC

• CODABAR (NW-7)

• CODE 39

• CODE 93

• CODE 128

2.8 Operator’s Manual MEK-7300K

2. PREPARATION

Before turning on the analyzer power by pressing the [Power] key on the front
panel, connect the bar code reader cable to the bar code socket on the rear panel.
When the bar code reader is connected, the power is supplied through pin 9 of
the bar code reader socket.

NOTE

Do not connect instruments other than ZK-820V hand-held bar code
reader to the bar code reader socket.

To set the various settings for the hand-held bar code reader, read the bar code
attached to the bar code reader manual with the hand-held bar code reader
within 15 seconds after turning the bar code reader power on. After changing the
settings, turn the bar code reader power off.

You can also use the bar codes in “Bar Codes for Using the ZK-820V Hand-held
Bar Code Reader” in Appendix B.

2

Operator’s Manual MEK-7300K 2.9

2. PREPARATION

Equipotential ground terminal

Connecting the Power Cord and Grounding the Analyzer

Connecting the Power Cord

AC source socket

Equipotential Grounding

CAUTION

Only use the provided power cord. Using other power cords may

result in electrical shock or injury to the operator.

Connect the provided power cord to the AC SOURCE socket on the rear panel
and plug the cord into a 3-prong AC outlet.

CAUTION

When several medical instruments are used together, ground all

instruments to the same one-point ground. Any potential difference

between instruments may cause electrical shock to the operator.

When more than one electrical instrument is used, there may be electrical
potential difference between the instruments. The potential difference between
the instruments may cause current to ow to the patient connected to the
instruments, resulting in electrical shock.

Always perform equipotential grounding when required. When equipotential
grounding is required, connect the equipotential ground terminal on the
rear panel of the analyzer to the equipotential ground terminal on the wall
(equipotential grounding system) with the equipotential grounding lead (potential
equalization conductor).

2.10 Operator’s Manual MEK-7300K

Connecting Tubes and Installing Reagents

In order for the analyzer to operate correctly, you must install all reagents and the
waste tube before the power is turned ON.

Materials Required

• Powder-free gloves, lab coat, safety glasses

• ISOTONAC•3 diluent

• CLEANAC detergent

• CLEANAC•3 detergent

• Hemolynac•3N lysing reagent

• Hemolynac•5 lysing reagent

• Reagent inlet tubes and waste outlet tube

• Waste container (or appropriate drain)

• 2 L container

• Lint-free cloth

2. PREPARATION

2

CAUTION

Only use Nihon Kohden recommended reagents and consumables.

Otherwise the measurement result cannot be guaranteed and

incorrect reagent concentration can cause equipment damage.

CAUTION

If reagent has been frozen, it must not be used.

Diluent (ISOTONAC•3)

NOTE

• If the diluent contacts the skin or eyes or is swallowed, wash

immediately and thoroughly with water.

• Use the diluent at room temperature 15 to 30ºC (59 to 86ºF).

• When the temperature of the diluent decreases to less than 15ºC

(59ºF), it may influence WBC differential parameters (NE, LY, MO, EO,

BA) but not influence the WBC count.

• Do not put new diluent in old diluent bottles.

Operator’s Manual MEK-7300K 2.11

2. PREPARATION

Detergent

CLEANAC•3

WARNING

Do not allow CLEANAC•3 detergent to come into

contact with acid. Contact with acids can cause

the release of poisonous chlorine gas.

CAUTION

If the CLEANAC•3 detergent contacts the skin,

eyes or mouth, wash thoroughly and immediately

with water and see a physician.

Store the detergent at room temperature 15 to 30ºC (59 to 86ºF).

CLEANAC

Do not swallow the CLEANAC detergent. If swallowed, see a physician

immediately.

WARNING

Do not swallow the CLEANAC•3 detergent. If

swallowed, see a physician immediately.

NOTE

WARNING

Store the detergent at room temperature 15 to 30ºC (59 to 86ºF).

Lysing Reagent

Hemolynac•3N

CAUTION

Do not swallow the Hemolynac•3N lysing reagent.

If swallowed, see a physician immediately.

CAUTION

If the Hemolynac•3N lysing reagent contacts the

skin, eyes or mouth, wash thoroughly and

immediately with water and see a physician.

Hemolynac•5

NOTE

CAUTION

Avoid reagent contact with the skin. If it contacts

the skin or eyes, wash thoroughly with water and

see a physician immediately.

CAUTION

Do not swallow the Hemolynac•5 lysing reagent.

If swallowed, see a physician immediately.

2.12 Operator’s Manual MEK-7300K

If the Hemolynac•5 lysing reagent contacts the

skin, eyes or mouth, wash thoroughly and

immediately with water and see a physician.

CAUTION

Connecting Tubes

2. PREPARATION

NOTE

• Use the reagent before the expiration date on the package or label and

within the specified period after opening.

• Use only the specified cap for the hemolysing reagent. Otherwise the

reagent concentration changes and it affects the measurement.

• Do not use the reagent outside of the designated laboratory.

• Completely seal the cap of the reagent when storing it.

• Read the instructions thoroughly to use the hemolysing reagent.

• Do not put new lysing reagent in old lysing reagent bottles.

NOTE

• When connecting tubes or replacing reagents, do not let dust or

bacteria enter the port or bottle. The analyzer may get damaged.

• Do not squeeze or bend the tubes. The tube may get accidentally

disconnected or the analyzer may get damaged.

• Install the diluent and detergent container at the same level.

• Install the reagents so they do not block the vent hole for the fan.

• If necessary, cut the diluent tube and reagent tube to an appropriate

length (2.0 m or less) when the length of the tube does not fit. For the

lysing reagent and detergent, use only the specified tubes.

• Follow the instructions on each package for handling the diluent and

detergent.

• For handling the lysing reagent, refer to the manual of the reagent.

• After installing the reagents, do not block the vent hole on the bottle

cap.

2

Lysing reagent
(Hemolynac•3N)

Lysing reagent
(Hemolynac•5)

Detergent
(CLEANAC)

Waste container

Diluent

Detergent
(CLEANAC•3)

Operator’s Manual MEK-7300K 2.13

2. PREPARATION

Diluent Tube

NOTE

Try to keep the diluent container at the same level as the analyzer.

1. Connect the diluent tube (marked blue) to the ISO3
inlet on the right side panel.

2. Pass the diluent tube through the 18 L container cap.

18 L container cap

Diluent tube
(marked blue)

Diluent (18 L)

18 L tube assy 2

Detergent Tube

Only use the specified detergent tubes for the detergent.

For performing strong clean, use CLEANAC•3 detergent. For other purposes,
use CLEANAC detergent.

CLEANAC Detergent

Try to keep the CLEANAC detergent container at the same level as the

analyzer.

3. Connect the end of the diluent tube to the 18 L tube
assy 2.

4. Put the 18 L tube assy 2 into the diluent container
and tighten the 18 L container cap.

NOTE

NOTE

Detergent tube
(marked green)

CLEANAC
detergent (5 L)

18 L container cap

Cleanac tube
assy

1. Connect the detergent tube (marked green) to the
CLN inlet on the right side panel.

2. Pass the detergent tube through the 18 L container
cap.

3. Connect the end of the detergent tube to the cleanac
tube assy.

4. Put the cleanac tube assy into the CLEANAC
container and tighten the 18 L container cap.

2.14 Operator’s Manual MEK-7300K

2. PREPARATION

CLEANAC•3 Detergent

NOTE

Place the CLEANAC•3 detergent container at the same level as the

analyzer.

1. Connect the cleanac tube 8 (marked white) to the
CLN3 inlet on the side panel.

2. Pass the cleanac tube 8 through the 18 L cap.

2

Cleanac tube 8
(marked white)

CLEANAC•3 detergent

Detergent inlet
(for CLEANAC•3)

18 L cap

Cleanac tube
assy (8222)

Detergent tube
(marked white)

1 L cap

1 L tube assy

3. Connect the end of the cleanac tube 8 to the cleanac
tube assy (8222).

4. Put the cleanac tube assy (8222) into the
CLEANAC•3 container and tighten the 18 L cap.

When the CLEANAC•3 (1 L) is used

1. Connect the detergent tube (marked white) to the
CLN3 inlet on the side panel.

2. Pass the end of the detergent tube into the 1 L cap
and attach the 1 L tube assy.

3. Put the 1 L tube assy into the tank and tighten the
cap.

Operator’s Manual MEK-7300K 2.15

2. PREPARATION

When the YZ-0066 reagent bottle set is used

1. Pour the detergent (CLEANAC•3) into the 2 L tank.

Detergent inlet
(for CLEANAC•3)

2 L tank

Detergent tube
(marked white)

MEK cap

Tube assy

Lysing Reagent Tube

• Place the lysing reagent container at the same level as the analyzer.

• Only use the specified lysing reagent tubes for the lysing reagent.

Hemolynac•3N

2. Connect the detergent tube (marked white) to the
CLN3 inlet on the right side panel.

3. Pass the end of the detergent tube into the MEK cap
and attach the tube assy.

4. Put the tube assy into the tank and tighten the cap.

NOTE

1. Replace the lysing reagent cap with the
Hemolynac•3 cap (yellow) and tighten the cap.

Lysing reagent inlet
(for Hemolynac•3N)

Hemolynac•3 cap (yellow)

Place the tip of the tube at
the bottom of the bottle.

Hemolynac•3
tube (570)
(marked yellow)

Attach the tape
and cap tightly.

2. Connect the Hemolynac•3 tube (570) (marked
yellow) to the HEMO3N inlet on the right side
panel.

3. Put the other end of the tube into the lysing reagent
container through the Hemolynac•3 cap (yellow).
Attach the tape and cap tightly and place the tip of
the tube at the bottom of the bottle.

4. When using the reagent tray, place the lysing reagent
container on the upper shelf of the reagent tray.

2.16 Operator’s Manual MEK-7300K

Hemolynac•5

2. PREPARATION

1. Replace the hemolysing reagent cap with the
Hemolynac•5 cap (black) and tighten the cap.

2

Lysing reagent inlet
(for Hemolynac•5)

Hemolynac•5 cap (black)

Place the tip of the tube at
the bottom of the bottle.

Hemolynac•5
tube (570)
(marked black)

Attach the tape
and cap tightly.

Waste Fluid Tube

Try to keep the waste container at the same level as the analyzer.

2. Connect the Hemolynac•5 tube (570) (marked black)
to the HEMO5 inlet on the side panel.

3. Put the other end of the tube into the hemolysing
reagent container through the Hemolynac•5 cap
(black). Attach the tape and cap tightly and place the
tip of the tube at the bottom of the bottle.

NOTE

1. Connect the waste tube (marked red) to the waste
outlet on the right side panel.

Waste tube
(marked red)

Waste container

MEK cap

Waste tube assy

2. Pass the waste tube through the MEK cap.

3. Connect the waste tube to the waste tube assy.

4. Insert the waste tube assy into the waste container
and tighten the MEK cap.

Operator’s Manual MEK-7300K 2.17

2. PREPARATION

Turning the Laser Switch On

1. Insert the laser key into the laser switch hole on the
right side panel.

2. Turn the laser switch to ON.

NOTE

• When the measurement is performed with the

laser switch turned off, CBC can be measured but

an alarm is displayed and there is no WBC 5 part

differential scattergram and data.

• Store the key in a safe place.

2.18 Operator’s Manual MEK-7300K

2. PREPARATION

Loading Recording Paper in the WA-730V Printer Unit (Option)

NOTE

• The WA-730V printer unit is optional (built-in type).

• Only use the specified recording paper.

1. Open the printer door.

2. Set the recording paper in the paper tray in the direction as shown.

2

3. Insert the recording paper into the slot.

4. Press the [ Feed] key on the front panel until the recording paper comes
out from the printer unit.

5. Put the paper through the opening of the door and close the door.

6. Cut the extra paper.

Operator’s Manual MEK-7300K 2.19

2. PREPARATION

Main power switch

Main power lamp

Turning Power On

Check Before Turning Power On

Check the following items before turning on the power.

Accessories and
consumables

Connection and settings

Appearance No scratches, dirt or leakage (especially in the

Quality control Run quality control by measuring a hematology

Use after long term
storage

Item Check

Sufcient diluent, detergent and lysing reagent.

Power cord is connected properly.
Grounding lead is connected properly when

equipotential grounding is required.
Tubes are connected properly.
Diluent, detergent and lysing reagent containers are

connected and have no dust in them.
Waste container is in place and empty.
Enough recording paper in the optional printer.
External instruments (e.g. PC and printer) are

properly connected.

measurement baths, sub baths and pump tube).
No key or switch is broken.
No damage to the power cord.
Waist container is not full.
Analyzer is not wet.

control.
Aperture caps are clean.
Pump tube and pinch valve tube are not broken and

not disconnected.

Turning On the Power

2.20 Operator’s Manual MEK-7300K

1. Conrm that the analyzer, printer (optional), and ZK-820V hand-held bar
code reader (optional) power cords are connected to grounded power outlets.

2. Set the printer power switch ON.

3. Press the [Main power] switch on the rear panel to ON. The [Main power
lamp] on the front panel lights.

Always leave the main power ON except for storage and transport of the

analyzer.

4. Press the [Power] key on the front panel ON. The [Power lamp] lights
and the screen illuminates within 15 to 30 seconds. Cleaning of the uid
path, priming and circuit self-check are automatically performed, and the
“Checking detergent” message appears. When the laser switch on the right
side panel is turned on, the laser lamp also lights.

Power keyPower lamp

2. PREPARATION

If there is an error, “Fail” appears on the screen.

After priming operation is completed, the Ready screen appears.

2

NOTE

If an error message appears, refer to “Alarm Messages” in Section 10.

5. Perform strong cleaning by pressing the Operation key → Strong clean key.

6. After strong cleaning, press the OK key.

7. Press the Ready key to display the Ready screen.

Operator’s Manual MEK-7300K 2.21

2. PREPARATION

Checking the Date and Time Settings

After turning the analyzer ON, date and time must be checked. To correct the
date and time on the upper right corner of the screen, refer to “Setting Date and
Time” in Section 5.

Cleaning the Analyzer

After installing the analyzer, turn the power on to rell reagents into the analyzer.

After installing the analyzer or long term storage, perform strong cleaning. Refer
to “Using the Analyzer after Storage” in section 9.

Check After Turning On the Power

Check the following items after turning on the power to start operating safely and
properly. If any problem is detected, take the proper countermeasure according to
Section 10 “Messages and Troubleshooting”.

Item Check

Turning on the power

Basic operation

After long term storage Perform Strong cleaning on the Operation screen.

At the start of the day, check that the date and time settings are correct.

There is no re, smoke or smell.
The analyzer is not too hot.
There is no electric shock.
The main power and power lamps light.
No alarm message is displayed on the screen.
The messages are displayed properly.
Keys and switches operate properly.
The touch screen keys function properly.
The lamps and LED indication are correct.
The measured background noise values are proper.
The measured hematology control values are proper.
The printer works properly.
The date and time are correct.
No alarm message is displayed on the screen during

operation.

NOTE

Optical Adjustment

After installing the analyzer, perform optical adjustment to measure samples
correctly. Refer to “Performing Optical Adjustment” in section 5.

2.22 Operator’s Manual MEK-7300K

Checking Daily Accuracy

Measuring Background Noise

2. PREPARATION

To assure measurement reliability, check the analyzer daily before measurement.
For details on the measurement reliability, refer to Section 11 “Quality Control”.

• Count the diluent to measure background noise.

• Count the MEK-5D hematology control to check accuracy.

Count the diluent to measure background noise. When using the analyzer
in closed mode, measure background noise in closed mode. When using the
analyzer in open mode, measure background noise in open mode. Background
noise increases in the following cases.

Problem Countermeasure

Old diluent. Germs begin to breed in the
diluent 60 days after opening.

Dust in the diluent container. Replace diluent.
Extremely high or low diluent

temperature (normal range is 15 to 30°C
(59 to 86°F)).

The sampling nozzle is clogged and
bubbles occur in the sub bath.

Replace diluent.

Adjust diluent temperature to 15 to
30°C (59 to 86°F).

Replace the sampling nozzle with a
new one.

2

There are two ways to measure background noise. One is by pressing the
[ Count] switch. The other is using the Background screen of the Other screen.
When measured on the Background screen, “Fail” appears beside the parameter
which is over the acceptable value on the result screen.

Measuring Background Noise in Closed Mode

Measuring by Pressing the [ Count] Switch

1. Select “Closed” for sampling mode on the Ready screen.

2. Press the [ Eject] key on the front panel. The tube holder opens.

3. Set an empty tube in the tube holder.

4. Close the tube holder. Diluent is measured. If the tube holder is closed
without setting a tube, measurement is not performed.

5. The tube holder opens automatically after nishing diluent measurement and
the result is displayed.

Operator’s Manual MEK-7300K 2.23

2. PREPARATION

Measuring on the Background Screen

1. Check that “Closed” is selected for sampling mode and press the Operation
key on the Ready screen.

2. Press the Background key on the Operation screen.

The “Measure background noise?” message appears on the screen.

3. Press the Yes key to measure background noise. The result is displayed after
the measurement is complete.

2.24 Operator’s Manual MEK-7300K

Measuring Background Noise in Open Mode

Measuring by Pressing the [ Count] Switch

1. Select “Open” for sampling mode on the Ready screen.

2. PREPARATION

2

2. Press the [ Count] switch to count the diluent. There is no need to aspirate
the diluent from the sampling nozzle. The result is displayed after the
measurement is complete.

Measuring on the Background Screen

1. Check that “Open” is selected for sampling mode and press the Operation
key on the Ready screen.

Operator’s Manual MEK-7300K 2.25

2. PREPARATION

2. Press the Background key on the Operation screen.

The “Measure background noise?” message appears on the screen.

3. Press the Yes key to measure background noise. The result is displayed after
the measurement is complete.

Results

The result is displayed on the screen after measurement.

Make sure that the values are less than or equal to the following values.
WBC: 0.2 (×103/µL)
RBC: 0.05 (×106/µL)
HGB: 0.1 (g/dL)
PLT: 10 (×103/µL)

When measured on the Background screen, “Fail” appears beside any parameter
which is over the acceptable value.

2.26 Operator’s Manual MEK-7300K

2. PREPARATION

Disregard the other parameter values because noise does not affect them.

Measuring Hematology Control

If the values are greater than the values listed above, check the following items,
press the [ Clean] key on the front panel to clean the uid path, and recount the
diluent.

• The diluent is clean.

• No bubbles in the diluent.

• The aperture caps are clean.

• The aperture caps are rmly attached.

• The measurement baths and sub baths are clean.

To check the measurement accuracy of the analyzer, measure a hematology
control.

NOTE

The hematology control is for controlling the accuracy of the analyzer. It is

made from healthy human blood. Because living cells are used, handle and

store the control correctly according to the explanation of the assay sheet.

2

1. On the Ready screen, set the measurement settings as follows.

• Sample type: Control
On the control select window, select the control to use and

press the OK key. When the Cancel is pressed, the Sample
mode returns to Normal.

• Measure mode: Normal

• Parameter: CBC + Diff

Operator’s Manual MEK-7300K 2.27

2. PREPARATION

Sampling nozzle

Count
switch

Set the control type as follows.

Hematology Cntrol Setting

MEK-5DN Normal

MEK-5DL Low

MEK-5DH High

2. Measure a hematology control.

NOTE

• Before measurement, make sure that the control setting matches

the control.

• After measurement, the Sample type returns to Normal.

Open mode

i) Remove the cap of the hematology control and put the sampling nozzle

into the control as shown in the illustration.

NOTE

Do not let the sampling nozzle touch the bottom of the

hematology control. This may prevent aspiration of the control.

ii) Press the count switch to start measurement. When the switch is pressed,

the sample is aspirated, diluted in the analyzer and measured. During
measurement, the “Measuring” message is displayed.

Put the nozzle to
this position.

NOTE

Do not lower the sample while it is being aspirated.

2.28 Operator’s Manual MEK-7300K

Eject key

Closed mode

Set the hematology control to the tube holder.

i) Press the eject [ ] key to open the tube holder.

ii) Set the hematology control into the tube holder.

2. PREPARATION

2

Tube holder

iii) Close the tube holder. The measurement automatically starts. During

measurement, the “Measuring” message is displayed.

3. Check that the measurement value is within the range of the expected value
on the assay sheet.

Operator’s Manual MEK-7300K 2.29

2. PREPARATION

Preparing SD Card

Use a QM-001D or specied SD memory card for this analyzer.

• Only use the specified SD card. Otherwise, data cannot be saved or the

analyzer may get damaged.

• The operation of a mini or micro SD card with conversion adapter

cannot be guaranteed.

• An SDHC or SDXC memory card cannot be used.

Safety Information about the SD Card

For handling and safety information about SD card, refer to the SD card

manual together with this manual.

NOTE

NOTE

Handling and Storage

• Keep the SD card slot clean. If dust gets into the slot, the SD card will

not function.

• Do not handle the SD card while eating or drinking.

• Do not get the SD card wet.

• Do not give impact to the SD card by dropping or bending.

• Do not expose the SD card to direct sunlight or leave it in a high

temperature place.

Environmental Conditions

• Storage environment
Temperature: –20 to +65°C (–4 to +149°F)
Humidity: less than 95% (noncondensing)

• Operating environment: same as the analyzer

Data Processing

• Initialize the SD card on this hematology analyzer. An SD card that was

initialized on other instruments cannot be used. Refer to “Formatting SD

Card” in Section 5.

• Do not use an unspecified instrument to save data to the SD card. The

card may get damaged.

• Do not remove the SD card or turn off the power during data

processing. The card may get damaged.

2.30 Operator’s Manual MEK-7300K

Inserting and Removing the SD Card

2. PREPARATION

NOTE

• When inserting or removing a SD card, turn off the power of the

hematology analyzer. The data in the card may get damaged or the

hematology analyzer might not operate correctly.

• Do not bend the SD card or insert it at an angle.

Insert the SD card into the SD card slot so that the label on the SD card is toward
front.

When removing the SD card, push the card once and remove it.

2

Operator’s Manual MEK-7300K 2.31

Section 3 Principles of Operation

Operation Theory ................................................................................................................................................. 3.2

Electric Cell Counting ................................................................................................................................3.2

Counting Method ............................................................................................................................. 3.2

Red Blood Cell and Platelet Counting ............................................................................................. 3.3

Principle of Hydraulic Operation ................................................................................................................ 3.3

Hemoglobin Measurement ........................................................................................................................ 3.4

Chemical Processing ......................................................................................................................3.4

Spectrophotometric Measurement Method .....................................................................................3.4

Principle of WBC Differential Operation .................................................................................................... 3.5

Measurement Results .......................................................................................................................................... 3.6

WBC Scattergrams .................................................................................................................................... 3.6

Histograms ................................................................................................................................................ 3.7

Vertical Axis Type for the Histogram ................................................................................................ 3.7

RBC (Red Blood Cell) Distribution Histogram ................................................................................. 3.8

PLT (Platelet) Distribution Histogram .............................................................................................. 3.8

Flags .......................................................................................................................................................... 3.9

3

Operator’s Manual MEK-7300K 3.1

3. PRINCIPLES OF OPERATION

Operation Theory

Electric Cell Counting

Counting Method

The hematology analyzer uses the volumetric impedance method of cell counting.

In this method, an electrolytic solution (diluent) containing suspended blood cells
is aspirated through the aperture. Two electrodes, an internal electrode and an
external electrode, are located close to the aperture and a constant current ows
between them. When a blood cell passes through the aperture, the resistance
between the electrodes momentarily increases and a very small voltage change
occurs corresponding to the resistance. The voltage signal is amplied and is sent
to the electronic circuit.

A threshold circuit eliminates signals caused by electrical noise, dust, debris and
particles which are smaller or larger than blood cells.

Detection circuit

Constant
current circuit

Electrodes

Electrolytic solution

Aperture

To nd the peak values, the signals are sent to the A/D converter. The acquired
data is stored in memory for each individual peak value. The data is corrected by
the CPU and displayed on the screen.

CPU

Amplifier Threshold circuit

A/D converter

Memory

The number of signals for each size cell is stored in memory as a histogram.
Counted cells of RBC and PLT can have overlap sizes so the CPU can
discriminate the count for each type of cell. See the “Red Blood Cell and Platelet
Counting” section.

Sometimes two or more cells pass through the aperture at the same time. This is
called coincidence. When the sample solution is sufciently diluted and mixed,
this can be statistically predicted to a high degree of accuracy. The software
contains a coincidence correction table to compensate for this.

3.2 Operator’s Manual MEK-7300K

Count

3. PRINCIPLES OF OPERATION

Red Blood Cell and Platelet Counting

Data of the RBC and PLT pulses are stored in the memory as a histogram.

Normal blood shows a clear separation between the PLT volume range and RBC
volume range (Fig. A) so an accurate PLT count is easily acquired. However,
when abnormal blood such as microcytic blood is counted (Figs. B and C),
the separation is unclear. In these cases, for accurate PLT counting, the CPU
determines the PLT and RBC distribution pattern, and sets the upper threshold
(PLT HI) to the lowest count.

Count

3

PLT LO*

Count

volume range

PLT LO*

Normal blood

PLT HI*

PLT

PLT

Macro platelet

PLT

PLT

volume range

Fig. A

RBC

volume range

PLT HI*

Fig. C

RBC

RBC

volume range

amplitude

(volume)

RBC

amplitude

(volume)

Microcytic blood (Microcythemia)

PLT LO*

volume range

PLT HI*

PLT

PLT

volume range

Fig. B

PLT LO*: Lower threshold
PLT HI*: Upper threshold

RBC

RBC

amplitude

(volume)

Principle of Hydraulic Operation

For accurate counting of the blood cells in a diluent solution, a constant volume
of solution must be aspirated through the aperture. The manometer controls this
volume by measuring diluent level by an optical sensor and aspirating the diluent
by the rotary pump.

Aspiration and dispensing of the sample are performed alternately by an
electromagnetic valve. The waste uid is drained from the hematology analyzer
by the rotary pump.

The priming of diluent and cleaning with detergent are also performed by the
electromagnetic valves.

The CPU controls the electromagnetic valves, pump rotations and rotation
direction. If an air bubble enters the manometer, the CPU generates an alarm
sound and displays an error message on the screen.

Operator’s Manual MEK-7300K 3.3

3. PRINCIPLES OF OPERATION

Hemoglobin Measurement

Chemical Processing

A hemolysing reagent is added to the diluted blood sample to break the red blood
cell membrane and release the hemoglobin.

Spectrophotometric Measurement Method

The hemoglobin is measured by spectrophotometry. This method measures the
optical density of the sample solution. The optical density is proportional to the
amount of hemoglobin in the sample solution.

Spectrophotometric measurement is based on the principle that different
materials absorb different amounts of different wavelengths of light. When light
strikes a material, some of the light is absorbed by the material and some passes
through. The amount of absorbed light at each frequency forms a unique optical
“ngerprint” for that material.

In this hematology analyzer, hemoglobin is measured in the measurement
baths. An LED shines one wavelength of light through the sample solution. A
photodiode receives the light which is not absorbed by the solution. The amount
of received light is converted to an electric signal which is amplied by the
preamplier. The amplied signal is sent to the A/D converter.

The amplied signals from the sample and diluent are required for measuring
the hemoglobin concentration. The sample data is sent to the CPU. The CPU
converts the ratio of these data to logarithmic data, multiplies it by the calibration
coefcient and displays the result on the LCD.

After measurement, the sample and diluent are drained from the hematology
analyzer. The sample is a highly concentrated protein solution. If the sample is
left in the measurement baths for a long time, the measurement baths gradually
become dirty. To prevent this problem, the measurement baths are automatically
cleaned by dispensing the diluent after each measurement.

Hemoglobin absorption characteristics

Absorbance

(0.D)

0.5

Cyanmet­hemoglobin

Non-cyan
surfactant

Oxidized hemoglobin

520500

540 560 580

Wavelength (nm)

3.4 Operator’s Manual MEK-7300K

Principle of WBC Differential Operation

The hematology analyzer uses the light scatter technique to differentiate WBC
into neutrophil, lymphocyte, monocyte, eosinophil and basophil counts.

3. PRINCIPLES OF OPERATION

The diluted blood sample is injected into the ow cell. Blood cells pass through
the sensing zone in a single le. A laser beam through the sensing zone is
scattered by the passing cells and the scattered light is detected. The angle and
intensity of scattered light depend on the volume and characteristics of the cell.
From this, WBC can be differentiated into 5 parts.

For details about the scattergrams, refer to “Measurement Results” later in this
section.

Laser

Granularity

Complexity

Size

3

Sample

DiluentDiluent

Operator’s Manual MEK-7300K 3.5

3. PRINCIPLES OF OPERATION

Measurement Results

WBC Scattergrams

There are 3 WBC scattergrams. You can compare 3 scattergrams at a time. You
can also enlarge one scattergram to examine it.

The main scattergram is divided into 3 areas.

Monocyte and
basophil count

Lymphocyte count

The lymphocyte count is acquired from the lymphocyte area.

The NE-EO scattergram is the neutrophil, eosinophil and ghost 2 area expanded
with the granularity as the horizontal axis. The neutrophil count and eosinophil
count can be acquired from this scattergram.

Neutrophil count

Neutrophil and
eosinophil count

Eosinophil count

The MO-BA scattergram is the monocyte and basophil area expanded with the
granularity as the horizontal axis. The monocyte count and basophil count can be
acquired from this scattergram.

3.6 Operator’s Manual MEK-7300K

3. PRINCIPLES OF OPERATION

Histograms

Monocyte count

Basophil count

Lymphocyte count

On the Result screen, you can view three histograms. You can examine the
histograms when anisocytosis occurs or hemolyzation is poor.

Numeric result

When touched,
it enlarges to
full screen.

3

Vertical Axis Type for the Histogram

Counted value (COUNTED): The vertical axis on the graph represents

numerical count values.

Percentage (%): The vertical axis on the graph is xed at

100% full scale regardless of the total counted
value. Therefore, the trend of particle volume
distribution can be easily judged even though
the total counted value is not shown.

(Count)

Counted Value

(%)

100%

(fL)

Percentage

The horizontal axis represents the blood cell volume.
(fL = 1 × 10

-15

L)

(fL)

Operator’s Manual MEK-7300K 3.7

3. PRINCIPLES OF OPERATION

RBC (Red Blood Cell) Distribution Histogram

The RDW indicates the deviation ratio of the red blood cell volumes on the
histogram.

RDW-CV (%)=

MCV

Standard deviation of red blood cell volumes (SD)

Mean cell volume (MCV)

SD

SD

RDW-SD (fL) = Standard deviation of red blood cell volumes (SD) × 4

(= 2 SD × 2)

2SD 2SD

(fL)

RDW-SD

PLT (Platelet) Distribution Histogram

The following parameters are automatically calculated from the PLT distribution
histogram.

• Platelet Crit (PCT)
The PCT indicates the ratio of the platelet total volume on the histogram to the

aspirated whole blood volume.

• Mean Platelet Volume (MPV)
The MPV indicates the mean of the platelets volume on the histogram.

• Platelet Distribution Width (PDW)
The PDW indicates the deviation ratio of the platelet volumes on the

histogram.

MPV

SD

SD

10

20

3.8 Operator’s Manual MEK-7300K

Flags

Flags are displayed when the measurement result is above or below the threshold
for each item. The following list is the factory default settings. You can change
the threshold on the Flags screen of the Settings screen. Refer to “Flag Settings”
in Section 5.

You can enlarge ags by pressing the ag on the Result screen.

Close the enlarged
ag window.

3. PRINCIPLES OF OPERATION

3

Change the page.

Flag Appears when

WBC

• Leukocytosis WBC is above 18 × 103/µL

• Leukopenia WBC is below 2.5 × 103/µL

• Neutrophilia NE is above 11 × 103/µL

• Neutropenia NE is below 1.0 × 103/µL

• Lymphocytosis LY is above 4.0 × 103/µL

• Lymphopenia LY is below 0.8 × 103/µL

• Monocytosis MO is above 1.0 × 103/µL

• Eosinophilia EO is above 0.7 × 103/µL

• Basophilia BA is above 0.2 × 103/µL

• Blasts Presence of blasts is suspected

• Immature Gr Presence of immature granulocytes is suspected

• Left Shift Left shifted cells

• Atypical Ly Presence of atypical lymphocytes is suspected

• Small Nucleated Cell Presence of small nucleated cells is suspected

• Ly-Mo Interference Overlap of population of lymphocytes and monocytes
is suspected

• Ne-Eo Interference Overlap of population of neutrophils and eosinophils
is suspected

RBC

• Erythrocytosis RBC is above 6.5 × 106/µL

• Anemia HGB is below 10.0 g/dL

• Anisocytosis RDW-CV is above 20.0%

• Microcytosis MCV is below 70 fL

• Macrocytosis MCV is above 110 fL

• Hypochromia MCHC is below 29.0 g/dL

• Abnormal MCHC MCHC is below 28.0 g/dL or above 38.0 g/dL

Operator’s Manual MEK-7300K 3.9

3. PRINCIPLES OF OPERATION

PLT

• Thrombocytosis PLT is above 600 × 103/µL

• Thrombocytopenia PLT is below 60 × 103/µL

• PLT Clumps Presence of PLT clumps is suspected

• PLT-RBC Interference Overlap of population of PLT and RBC is suspected

• If “C” appears on the right side of the WBC or PLT data, the PLT may be
coagulated.

If “*” appears on the right side of the measurement value, the measurement
result might not be reliable. Check the sample with the manual differential
counts.

3.10 Operator’s Manual MEK-7300K

Section 4 Performance Characteristics

and Specications

Specifications ......................................................................................................................................................4.2

Measured Parameters, Ranges and Reproducibility to Specimen ............................................................ 4.2

Detection Method ...................................................................................................................................... 4.2

Standardization Analysis Method ..............................................................................................................4.3

Dilution Ratio .............................................................................................................................................4.3

Counting Time ...........................................................................................................................................4.3

Display ....................................................................................................................................................... 4.3

Data Storage ............................................................................................................................................. 4.3

Environmental Conditions .........................................................................................................................4.3

Power Requirements ................................................................................................................................. 4.4

Dimensions and Weight ............................................................................................................................. 4.4

Electromagnetic Compatibility ...................................................................................................................4.4

Safety ........................................................................................................................................................ 4.4

Bar Code Specifications ............................................................................................................................4.5

Transfer Format PC (V03-01) .............................................................................................................................. 4.6

Transfer Example to a PC..........................................................................................................................4.6

Transfer Format PC (V03-02) .............................................................................................................................. 4.8

Transfer Example to a PC..........................................................................................................................4.8

Transfer Format PC (V05-01) ............................................................................................................................ 4.10

Transfer Example to a PC........................................................................................................................4.10

ASTM Communication ....................................................................................................................................... 4.13

4

Operator’s Manual MEK-7300K 4.1

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Specifications

Measured Parameters, Ranges and Reproducibility to Specimen

Specications except WBC population were determined using hematology control blood (MEK-5DN), counted 10 times
consecutively.

Measured Parameters Measuring Range

WBC: White blood cell count 0 to 299 × 103/µL within 2.0%CV
NE%: Neutrophil percent 0 to 99.9% within 5.0%CV
LY%: Lymphocyte percent 0 to 99.9% within 5.0%CV
MO%: Monocyte percent 0 to 99.9% within 12.0%CV
EO%: Eosinophil percent 0 to 99.9% within 20.0%CV

BA%: Basophil percent 0 to 99.9%

NE: Neutrophil count 0 to 299 × 103/µL
LY: Lymphocyte count 0 to 299 × 103/µL
MO: Monocyte count 0 to 299 × 103/µL
EO: Eosinophil count 0 to 299 × 103/µL
BA: Basophil count 0 to 299 × 103/µL
RBC: Red blood cell count 0 to 14.9 × 106/µL within 1.5%CV
HGB: Hemoglobin concentration 0 to 29.9 g/dL within 1.5%CV
HCT: Hematocrit 0 to 99.9% —
MCV: Mean cell volume 20 to 199 fL within 1.0%CV
MCH: Mean cell hemoglobin 10 to 50 pg

RDW-CV: Red blood cell distribution width 0 to 50%
PLT: Platelet count 0 to 1490 × 103/µL within 4.0%CV
PCT: Platelet crit 0 to 2.9%

PDW: Platelet distribution width 0 to 50%

within CV30.0% (>2%) or average value ±1% (0
to 2%)

Reproducibility to Specimen
(CV: Coefficient of Variation)

—

—MCHC: Mean cell hemoglobin concentration 10 to 50 g/dL

—MPV: Mean platelet volume 0 to 20.0 fL

Detection Method

Blood cell count: Electrical resistance detection
Hemoglobin: Surfactant method (colorimetric method)
Hematocrit: Histogram calculation
WBC population: Light scatter by laser
Platelet crit: Histogram calculation
RBC distribution width: Histogram calculation
Platelet distribution width: Histogram calculation

4.2 Operator’s Manual MEK-7300K

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Standardization Analysis Method

WBC: ICSH1988 ICSH: The assignment of values to fresh blood used for calibrating automated blood cell counters.

Clin Lab Haematol, 10:203-212, 1988

RBC: ICSH1988 ICSH: The assignment of values to fresh blood used for calibrating automated blood cell counters.

Clin Lab Haematol, 10:203-212, 1988

HGB: NCCLS H15-A2 H15-A2: Reference and Selected Procedures for the Quantitative Determination of

Hemoglobin in Blood Second Edition; Approved Standard (1994)

HCT: NCCLS H7-A2 H7-A2: Procedure for Determining Packed Cell Volume by the Microhematocrit

Method Second Edition; Approved Standard (1993)

PLT: Brecher & Cronkite Method: Morphology and enumeration of human blood platelets, J Appl Physiol 3 365 (Dec)

1950; Brecher G, Cronkite EP

Dilution Ratio

• Venous blood
Sample volume: 55 μL (for 23 parameters)/30 μL (for WBC, RBC, HGB, HCT, MCV, MCH, MCHC, and PLT)
WBC/HGB: 200:1
RBC/PLT: 40,000:1

4

• Pre-dilution blood
Sample volume: 10 μL 20 μL
WBC/HGB: 1200:1 600:1
RBC/PLT: 240,000:1 120,000:1

• Capillary mode
Sample volume: 10 µL
WBC/HGB: 600:1
RBC/PLT: 120,000:1

Counting Time

Open mode: 63 s/sample (from measurement start to data display)
Closed mode: 75 s/sample (from measurement start to data display)

Display

Display: 10.4 inch, LCD with backlight and touch screen keys
Resolution: 800 × 600 dots
Screen size: approx. 211.2 × 158.4 mm
Display contents: Numerical data, scattergrams, histograms, measuring conditions, alarm message and other messages,

touch screen keys

Data Storage

Numerical data for all counted parameters for up to 400 samples and histograms and scattergrams for up to 50 samples

Environmental Conditions

Storage temperature: –20 to +60ºC (–4 to +140°F)
Operating temperature: 15 to 30ºC (59 to 86°F)
Storage humidity: 10 to 95%
Operating humidity: 30 to 85% (noncondensing)
Storage atmospheric pressure: 70 to 106 kPa
Operating atmospheric pressure: 70 to 106 kPa
Operating altitude: less than 3000 m

Operator’s Manual MEK-7300K 4.3

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Power Requirements

Power requirements: 110 to 240 V ± 10% AC, 50/60 Hz
Power consumption: 250 VA

Dimensions and Weight

Dimensions: 382 W × 465 D × 532 H (mm)
Net weight: approx. 35 kg

Electromagnetic Compatibility

IEC 61326-1: 2005
IEC 61326-2-6: 2005
EN 61326-1: 2006
EN 61326-2-6: 2006

CISPR11: Edition 4: 2003, Group 1, Class B
EN 55011: 2007, Group 1, Class B

The power supply short interruption test is performed through a transformer which has at least three times the power
capacity of the instrument.

Safety

Safety standards: IEC 61010-1 2nd Edition: 2001
EN 61010-1 2nd Edition: 2001
IEC 61010-2-081: 2001
IEC 61010-2-101: 2002
EN 61010-2-101: 2002

Laser: IEC 60825-1: 2007
EN 60825-1: 2007

Type of protection against electrical shock: CLASS I EQUIPMENT
Degree of protection against harmful ingress of water: IPX0 (non-protected)
Degree of safety of application in the presence of a FLAMMABLE ANAESTHETIC MIXTURE WITH AIR, OR WITH
OXYGEN OR NITROUS OXIDE: EQUIPMENT not suitable for use in the presence of

FLAMMABLE ANAESTHETIC MIXTURE WITH AIR, OR

WITH OXYGEN OR NITROUS OXIDE
Mode of operation: CONTINUOUS OPERATION
EQUIPMENT types (classication): Indoor stationary EQUIPMENT
Pollution Degree: 2 EQUIPMENT

Requirements for marking of IN VITRO DIAGNOSTIC instruments: EN1658: 1996

4.4 Operator’s Manual MEK-7300K

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Bar Code Specifications

Bar Code Format

The following formats with or without check digits are acceptable:

• Industrial 2 of 5

• ITF

• JAN/EAN/UPC

• NW-7

• CODE 39

• CODE 93

• CODE 128

Bar Code Label Specications
Refer to Appendix B for complete information on bar code label formats, check digits and specications.

4

Operator’s Manual MEK-7300K 4.5

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Transfer Format PC (V03-01)

Transfer Example to a PC

Common data block “_” in the Transferred Data in Characters column indicates a blank (0x20).

No. of

Transfer Items Example

<Start> Common data block
Start of Text 0x02
Hematology analyzer information
Type “MEK-7222”
Parameter no “22”
Send data bytes “01024”
Sampling mode “MANUAL”
Parameter “CBC + Diff”
Sample code “01”

Sample label “BLOOD”

Rack location “MMM”
Seq# “0000001”
Software version “V01-01”

Analysis program version

Format version “V03-01”
Total data bytes “01536”
Data block pattern “1”
Reserve data
Measurement data

Date “20050101”

Time “153000”
ID “ABCDEFGH:0001”
WBC “6.2”
NE% “70.6”
LY% “21.2”
MO% “2.5”
EO% “5.4H”
BA% “0.3”
NE “4.4”
LY “1.3”
MO “0.2”
EO “0.2”
BA “0.0”
RBC “5.10”
HGB “14.4”
HCT “42.3”
MCV “86.2”
MCH “28.5”
MCHC “33.1”
RDW-CV “11.5”
PLT “280”
PCT “0.15”
MPV “7.2”
PDW “18.5”
Reserve data
Flag data (with flag +, without flag (space))
Leukocytosis “+”
Leukopenia “+”
Neutrophilia “+”
Neutropenia “+”
Lymphocytosis “+”
Lymphopenia “+”
Monocytosis “+”
Eosinophilia “+”
Basophilia “+”
Blasts “+”

Immature Granulocyte

Left Shift “+”

Atypical lymphocytes

Poor hemolyzation “+”

Small nucleated cell

Ly-Mo interference “+”
Ne-Eo interference “+”
Reserve data
Erythrocytosis “+”
Anemia “+”
Anisocytosis “+”
Microcytosis “+”
Macrocytosis “+”
Hypochromia “+”
Abnormal MCHC “+”
Reserve data
Thrombocytosis “+”
Thrombocytopenia “+”
PLT Clumps “+”

PLT-RBC interference

Reserve data
Reserve data
Reserve data
<End> Common data block
End of Text 0x03

“V04-02”

“+”

“+”

“+”

“+”

Transferred Data in Characters Transferred Data in Hexadecimal

Byte

1 STX 02

11 M E K - 7 2 2 2 CR 4D 45 4B 2D 37 32 32 32 20 20 0D

6 2 2 CR 20 20 20 32 32 0D

6 0 1 0 2 4 CR 30 31 30 32 34 0D
13 M A N U A L CR 4D 41 4E 55 41 4C 20 20 20 20 20 20 0D
13 C B C + D i f f CR 43 42 43 20 2B 20 44 69 66 66 20 20 0D

3 0 1 CR 30 31 0D

17 B L O O D CR

5 M M M CR 4D 4D 4D 20 0D
11 0 0 0 0 0 0 1 CR 30 30 30 30 30 30 31 20 20 20 0D

9 V 0 1

9 V 0 4

9 V 0 3

6 0 1 5

6 1 CR 31 20 20 20 20 0D

4 SP × 3 Byte + CR × 1 Byte 20 × 3 Byte + 0D × 1 Byte

17 2 0 0 5 CR 0 1 CR 0 1 CR CR

9 1 5 CR 3 0 CR 0 0 CR 31 35 0D 33 30 0D 30 30 0D
16 A B C D E F G H : 0 0 0 1 CR

7 6 . 2 CR 20 36 2E 32 20 20 0D

7 7 0 . 6 CR 37 30 2E 36 20 20 0D

7 2 1 . 2 CR 32 31 2E 32 20 20 0D

7 2 . 5 CR 20 32 2E 35 20 20 0D

7 5 . 4 H CR 20 35 2E 34 48 20 0D

7 0 . 3 CR 20 30 2E 33 20 20 0D

7 4 . 4 CR 20 34 2E 34 20 20 0D

7 1 . 3 CR 20 31 2E 33 20 20 0D

7 0 . 2 CR 20 30 2E 32 20 20 0D

7 0 . 2 CR 20 30 2E 32 20 20 0D

7 0 . 0 CR 20 30 2E 30 20 20 0D

7 5 . 1 0 CR 35 2E 31 30 20 20 0D

7 1 4 . 4 CR 31 34 2E 34 20 20 0D

7 4 2 . 3 CR 34 32 2E 33 20 20 0D

7 8 6 . 2 CR 38 36 2E 32 20 20 0D

7 2 8 . 5 CR 32 38 2E 35 20 20 0D

7 3 3 . 1 CR 33 33 2E 31 20 20 0D

7 1 1 . 5 CR 31 31 2E 35 20 20 0D

7 2 8 0 CR 20 32 38 30 20 20 0D

7 0 . 1 5 CR 30 2E 31 35 20 20 0D

7 7 . 2 CR 20 37 2E 32 20 20 0D

7 1 8 . 5 CR 31 38 2E 35 20 20 0D

210 SP × 209 Byte + CR × 1 Byte 20 × 209 Byte + 0D × 1 Byte

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D
14 SP × 13 Byte + CR × 1 Byte 20 × 13 Byte + 0D × 1 Byte

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D
10 SP × 9 Byte + CR × 1 Byte 20 × 9 Byte + 0D × 1 Byte

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

8 SP × 7 Byte + CR × 1 Byte 20 × 7 Byte + 0D × 1 Byte

400 SP × 399 Byte + CR × 1 Byte 20 × 399 Byte + 0D × 1 Byte

1 ETX 03

0 1 CR 56 30 31 2D 30 31 20 20 0D

­0 2 CR 56 30 34 2D 30 32 20 20 0D

­0 1 CR 56 30 33 2D 30 31 20 20 0D

­6 CR 30 32 35 33 36 0D

3

42 4C 4F 4F 44 20 20 20 20 20 20 20 20 20 20
20 0D

32 30 30 35 0D 30 31 0D 30 31 0D 20 20 20 20
20 0D

41 42 43 44 45 46 47 48 3A 30 30 30 31 20 20 0D

(ex. 20 0D)

4.6 Operator’s Manual MEK-7300K

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Extended data block “_” in the Transferred Data in Characters column indicates a blank (0x20).

Transfer Items Example

<Start> Extended data block
Start of Text 0x02 1 STX 02
Hematology analyzer information
Identifier “EXP” 4 E X P CR 45 58 50 0D
Send data bytes “00512” 6 0 0 5 1 2 CR 30 30 35 31 32 0D
Type “MEK-7222” 11 M E K - 7 2 2 2 CR 4D 45 4B 2D 37 32 32 32 20 20 0D
Unit no “1” 3 1 CR 20 31 0D
Work list data
Name “DAVID” 27 D A V I D ... … CR 44 41 56 49 44 … … 20 20 20 20 … … 0D
Sex “MALE” 7 M A L E CR 4D 41 4C 45 20 20 0D
Date of birth “19800219” 11 1 9 8 0 CR 0 2 CR 1 9 CR 31 39 38 30 0D 30 32 0D 31 39 0D
Age “22” 4 2 2 CR 20 32 32 0D
Department “INTERNAL” 14 I N T E R N A L CR 49 4E 54 45 52 4E 41 4C 20 20 20 20 20 0D

Physician “WATSON” 27 W A T S O N ... ... CR 57 57 41 54 53 4F … … 20 20 20 … … 0D

Operator name “” 9 CR 20 20 20 20 20 20 20 20 0D

Comments “No problem.” 129 N o p r o b l e m . ... 4E 4E 6F 20 70 72 6F 62 6C 65 6D

Normal range table no“0” 2 0 CR 30 0D
Work list flag “1” 2 1 CR 31 0D
Control mode flag “” 2 CR 20 0D
Reserve data for work list data 32 SP × 31 Byte + CR × 1 Byte 20 × 31 Byte + 0D × 1 Byte
Normal range setting data
WBC-LOW “4.0” 5 4 . 0 CR 20 34 2E 30 0D
WBC-HIGH “9.0” 5 9 . 0 CR 20 39 2E 30 0D
NE%-LOW “42.0” 5 4 2 . 0 CR 34 32 2E 30 0D
NE%-HIGH “85.0” 5 8 5 . 0 CR 38 35 2E 30 0D
LY%-LOW “11.0” 5 1 1 . 0 CR 31 31 2E 30 0D
LY%-HIGH “49.0” 5 4 9 . 0 CR 34 39 2E 30 0D
MO%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
MO%-HIGH “9.0” 5 9 . 0 CR 20 39 2E 30 0D
EO%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
EO%-HIGH “3.0” 5 3 . 0 CR 20 33 2E 30 0D
BA%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
BA%-HIGH “2.0” 5 2 . 0 CR 20 32 2E 30 0D
NE-LOW “1.7” 5 1 . 7 CR 20 31 2E 37 0D
NE-HIGH “7.7” 5 7 . 7 CR 20 37 2E 37 0D
LY-LOW “0.4” 5 0 . 4 CR 20 30 2E 34 0D
LY-HIGH “4.4” 5 4 . 4 CR 20 34 2E 34 0D
MO-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
MO-HIGH “0.8” 5 0 . 8 CR 20 30 2E 38 0D
EO-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
EO-HIGH “0.3” 5 0 . 3 CR 20 30 2E 33 0D
BA-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
BA-HIGH “0.2” 5 0 . 2 CR 20 30 2E 32 0D
RBC-LOW “3.80” 5 3 . 8 0 CR 33 2E 38 30 0D
RBC-HIGH “5.30” 5 5 . 3 0 CR 35 2E 33 30 0D
HGB-LOW “11.0” 5 1 1 . 0 CR 31 31 2E 30 0D
HGB-HIGH “17.0” 5 1 7 . 0 CR 31 37 2E 30 0D
HCT-LOW “36.0” 5 3 6 . 0 CR 33 36 2E 30 0D
HCT-HIGH “56.0” 5 5 6 . 0 CR 35 36 2E 30 0D
MCV-LOW “80.0” 5 8 0 . 0 CR 38 30 2E 30 0D
MCV-HIGH “100” 5 1 0 0 CR 20 31 30 30 0D
MCH-LOW “28.0” 5 2 8 . 0 CR 32 38 2E 30 0D
MCH-HIGH “36.0” 5 3 6 . 0 CR 33 36 2E 30 0D
MCHC-LOW “31.0” 5 3 1 . 0 CR 33 31 2E 30 0D
MCHC-HIGH “37.0” 5 3 7 . 0 CR 33 37 2E 30 0D
RDW-LOW “11.5” 5 1 1 . 5 CR 31 31 2E 35 0D
RDW-HIGH “16.5” 5 1 6 . 5 CR 31 36 2E 35 0D
PLT-LOW “120” 5 1 2 0 CR 20 31 32 30 0D
PLT-HIGH “380” 5 3 8 0 CR 20 33 38 30 0D
PCT-LOW “0.10” 5 0 . 1 0 CR 30 2E 31 30 0D
PCT-HIGH “1.00” 5 1 . 0 0 CR 31 2E 30 30 0D
MPV-LOW “5.0” 5 5 . 0 CR 20 35 2E 30 0D
MPV-HIGH “10.0” 5 1 0 . 0 CR 31 30 2E 30 0D
PDW-LOW “12.0” 5 1 2 . 0 CR 31 32 2E 30 0D
PDW-HIGH “18.0” 5 1 8 . 0 CR 31 38 2E 30 0D
<End> Extended data block
End of Text 0x03 1 ETX 03

No. of

Transferred Data in Characters Transferred Data in Hexadecimal

Byte

... CR … … 20 20 20 20 20 20 … … 0D

4

• “0x0D” is at the end of each item as separating character.

• Each item is entered by ASCII code. Only “Start of Text” and “ End of Text” are entered by control code.

• Each item is initialized at space (0x20). Unused items are also initialized at space and have “0x0D” in the end.

Operator’s Manual MEK-7300K 4.7

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Transfer Format PC (V03-02)

Transfer Example to a PC

Common data block “_” in the Transferred Data in Characters column indicates a blank (0x20).

No. of

Transfer Items Example

<Start> Common data block
Start of Text 0x02
Hematology analyzer information
Type “MEK-7222”
Parameter no “22”
Send data bytes “01024”
Sampling mode “MANUAL”
Parameter “CBC + Diff”
Sample code “01”

Sample label “BLOOD”
Rack location “MMM”

Seq# “0000001”
Software version “V02-01”

Analysis program version

Format version “V03-01”
Total data bytes “01536”
Data block pattern “1”
Reserve data
Measurement data

Date “20050101”
Time “153000”

ID “ABCDEFGH:0001”
WBC “6.2”
NE% “70.6”
LY% “21.2”
MO% “2.5”
EO% “5.4H”
BA% “0.3”
NE “4.4”
LY “1.3”
MO “0.2”
EO “0.2”
BA “0.0”
RBC “5.10”
HGB “14.4”
HCT “42.3”
MCV “86.2”
MCH “28.5”
MCHC “33.1”
RDW (RDW-CV) “11.5”
PLT “280”
PCT “0.15”
MPV “7.2”
PDW “18.5”
Primary type “MEK-7300,”

Primary format version

RDW-SD “40.3,”
IG “1.3,”
IG% “0.2,”
Reserve data
Flag data (with flag +, without flag (space))
Leukocytosis “+”
Leukopenia “+”
Neutrophilia “+”
Neutropenia “+”
Lymphocytosis “+”
Lymphopenia “+”
Monocytosis “+”
Eosinophilia “+”
Basophilia “+”
Blasts “+”

Immature Granulocyte

Left Shift “+”

Atypical lymphocytes

Poor hemolyzation “+”

Small nucleated cell

Ly-Mo interference “+”
Ne-Eo interference “+”
Reserve data
Erythrocytosis “+”
Anemia “+”
Anisocytosis “+”
Microcytosis “+”
Macrocytosis “+”
Hypochromia “+”
Abnormal MCHC “+”
Reserve data
Thrombocytosis “+”
Thrombocytopenia “+”
PLT Clumps “+”

PLT-RBC interference

Reserve data
Reserve data
Reserve data
<End> Common data block
End of Text 0x03

“V04-02”

“V03-02,”

“+”

“+”

“+”

“+”

Transferred Data in Characters Transferred Data in Hexadecimal

Byte

1 STX 02

11 M E K - 7 2 2 2 CR 4D 45 4B 2D 37 32 32 32 20 20 0D

6 2 2 CR 20 20 20 32 32 0D

6 0 1 0 2 4 CR 30 31 30 32 34 0D
13 M A N U A L CR 4D 41 4E 55 41 4C 20 20 20 20 20 20 0D
13 C B C + D i f f CR 43 42 43 20 2B 20 44 69 66 66 20 20 0D

3 0 1 CR 30 31 0D
17 B L O O D CR

5 M M M CR 4D 4D 4D 20 0D
11 0 0 0 0 0 0 1 CR 30 30 30 30 30 30 31 20 20 20 0D

9 V 0 1

9 V 0 4

9 V 0 3

6 0 1 5

6 1 CR 31 20 20 20 20 0D

4 SP × 3 Byte + CR × 1 Byte 20 × 3 Byte + 0D × 1 Byte

17 2 0 0 5 CR 0 1 CR 0 1 CR CR

9 1 5 CR 3 0 CR 0 0 CR 31 35 0D 33 30 0D 30 30 0D
16 A B C D E F G H : 0 0 0 1 CR

7 6 . 2 CR 20 36 2E 32 20 20 0D

7 7 0 . 6 CR 37 30 2E 36 20 20 0D

7 2 1 . 2 CR 32 31 2E 32 20 20 0D

7 2 . 5 CR 20 32 2E 35 20 20 0D

7 5 . 4 H CR 20 35 2E 34 48 20 0D

7 0 . 3 CR 20 30 2E 33 20 20 0D

7 4 . 4 CR 20 34 2E 34 20 20 0D

7 1 . 3 CR 20 31 2E 33 20 20 0D

7 0 . 2 CR 20 30 2E 32 20 20 0D

7 0 . 2 CR 20 30 2E 32 20 20 0D

7 0 . 0 CR 20 30 2E 30 20 20 0D

7 5 . 1 0 CR 35 2E 31 30 20 20 0D

7 1 4 . 4 CR 31 34 2E 34 20 20 0D

7 4 2 . 3 CR 34 32 2E 33 20 20 0D

7 8 6 . 2 CR 38 36 2E 32 20 20 0D

7 2 8 . 5 CR 32 38 2E 35 20 20 0D

7 3 3 . 1 CR 33 33 2E 31 20 20 0D

7 1 1 . 5 CR 31 31 2E 35 20 20 0D

7 2 8 0 CR 20 32 38 30 20 20 0D

7 0 . 1 5 CR 30 2E 31 35 20 20 0D

7 7 . 2 CR 20 37 2E 32 20 20 0D

7 1 8 . 5 CR 31 38 2E 35 20 20 0D

9 M E K - 7 3 0 0 , 4D 45 4B 2D 37 33 30 30 2C

7 V 0 3 - 0 2 , 56 30 33 2D 30 32 2C

7 4 0 . 3 , 34 30 2E 33 20 20 2C

7 1 . 3 , 20 31 2E 33 20 20 2C

7 0 . 2 , 20 30 2E 32 20 20 2C

173 × 172 Byte + CR × 1 Byte 20 × 172 Byte + 0D × 1 Byte

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D
14 SP × 13 Byte + CR × 1 Byte 20 × 13 Byte + 0D × 1 Byte

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D
10 SP × 9 Byte + CR × 1 Byte 20 × 9 Byte + 0D × 1 Byte

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

8 SP × 7 Byte + CR × 1 Byte 20 × 7 Byte + 0D × 1 Byte

400 SP × 399 Byte + CR × 1 Byte 20 × 399 Byte + 0D × 1 Byte

1 ETX 03

0 1 CR 56 30 31 2D 30 31 20 20 0D

­0 2 CR 56 30 34 2D 30 32 20 20 0D

­0 1 CR 56 30 33 2D 30 31 20 20 0D

­6 CR 30 32 35 33 36 0D

3

42 4C 4F 4F 44 20 20 20 20 20 20 20 20 20 20
20 0D

32 30 30 35 0D 30 31 0D 30 31 0D 20 20 20 20
20 0D

41 42 43 44 45 46 47 48 3A 30 30 30 31 20 20 0D

(ex. 20 0D)

4.8 Operator’s Manual MEK-7300K

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Extended data block “_” in the Transferred Data in Characters column indicates a blank (0x20).

Transfer Items Example

<Start> Extended data block
Start of Text 0x02 1 STX 02
Hematology analyzer information
Identifier “EXP” 4 E X P CR 45 58 50 0D
Send data bytes “00512” 6 0 0 5 1 2 CR 30 30 35 31 32 0D
Type “MEK-7222” 11 M E K - 7 2 2 2 CR 4D 45 4B 2D 37 32 32 32 20 20 0D
Unit no “1” 3 1 CR 20 31 0D
Work list data
Name “DAVID” 27 D A V I D ... … CR 44 41 56 49 44 … … 20 20 20 20 … … 0D
Sex “MALE” 7 M A L E CR 4D 41 4C 45 20 20 0D
Date of birth “19800219” 11 1 9 8 0 CR 0 2 CR 1 9 CR 31 39 38 30 0D 30 32 0D 31 39 0D
Age “22” 4 2 2 CR 20 32 32 0D
Department “INTERNAL” 14 I N T E R N A L CR 49 4E 54 45 52 4E 41 4C 20 20 20 20 20 0D

Physician “WATSON” 27 W A T S O N ... ... CR 57 57 41 54 53 4F … … 20 20 20 … … 0D

Operator name “” 9 CR 20 20 20 20 20 20 20 20 0D

Comments “No problem.” 129 N o p r o b l e m . ... 4E 4E 6F 20 70 72 6F 62 6C 65 6D

Normal range table no“0” 2 0 CR 30 0D
Work list flag “1” 2 1 CR 31 0D
Control mode flag “” 2 CR 20 0D
Reserve data for work list data 32 SP × 31 Byte + CR × 1 Byte 20 × 31 Byte + 0D × 1 Byte
Normal range setting data
WBC-LOW “4.0” 5 4 . 0 CR 20 34 2E 30 0D
WBC-HIGH “9.0” 5 9 . 0 CR 20 39 2E 30 0D
NE%-LOW “42.0” 5 4 2 . 0 CR 34 32 2E 30 0D
NE%-HIGH “85.0” 5 8 5 . 0 CR 38 35 2E 30 0D
LY%-LOW “11.0” 5 1 1 . 0 CR 31 31 2E 30 0D
LY%-HIGH “49.0” 5 4 9 . 0 CR 34 39 2E 30 0D
MO%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
MO%-HIGH “9.0” 5 9 . 0 CR 20 39 2E 30 0D
EO%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
EO%-HIGH “3.0” 5 3 . 0 CR 20 33 2E 30 0D
BA%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
BA%-HIGH “2.0” 5 2 . 0 CR 20 32 2E 30 0D
NE-LOW “1.7” 5 1 . 7 CR 20 31 2E 37 0D
NE-HIGH “7.7” 5 7 . 7 CR 20 37 2E 37 0D
LY-LOW “0.4” 5 0 . 4 CR 20 30 2E 34 0D
LY-HIGH “4.4” 5 4 . 4 CR 20 34 2E 34 0D
MO-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
MO-HIGH “0.8” 5 0 . 8 CR 20 30 2E 38 0D
EO-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
EO-HIGH “0.3” 5 0 . 3 CR 20 30 2E 33 0D
BA-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
BA-HIGH “0.2” 5 0 . 2 CR 20 30 2E 32 0D
RBC-LOW “3.80” 5 3 . 8 0 CR 33 2E 38 30 0D
RBC-HIGH “5.30” 5 5 . 3 0 CR 35 2E 33 30 0D
HGB-LOW “11.0” 5 1 1 . 0 CR 31 31 2E 30 0D
HGB-HIGH “17.0” 5 1 7 . 0 CR 31 37 2E 30 0D
HCT-LOW “36.0” 5 3 6 . 0 CR 33 36 2E 30 0D
HCT-HIGH “56.0” 5 5 6 . 0 CR 35 36 2E 30 0D
MCV-LOW “80.0” 5 8 0 . 0 CR 38 30 2E 30 0D
MCV-HIGH “100” 5 1 0 0 CR 20 31 30 30 0D
MCH-LOW “28.0” 5 2 8 . 0 CR 32 38 2E 30 0D
MCH-HIGH “36.0” 5 3 6 . 0 CR 33 36 2E 30 0D
MCHC-LOW “31.0” 5 3 1 . 0 CR 33 31 2E 30 0D
MCHC-HIGH “37.0” 5 3 7 . 0 CR 33 37 2E 30 0D
RDW-LOW “11.5” 5 1 1 . 5 CR 31 31 2E 35 0D
RDW-HIGH “16.5” 5 1 6 . 5 CR 31 36 2E 35 0D
PLT-LOW “120” 5 1 2 0 CR 20 31 32 30 0D
PLT-HIGH “380” 5 3 8 0 CR 20 33 38 30 0D
PCT-LOW “0.10” 5 0 . 1 0 CR 30 2E 31 30 0D
PCT-HIGH “1.00” 5 1 . 0 0 CR 31 2E 30 30 0D
MPV-LOW “5.0” 5 5 . 0 CR 20 35 2E 30 0D
MPV-HIGH “10.0” 5 1 0 . 0 CR 31 30 2E 30 0D
PDW-LOW “12.0” 5 1 2 . 0 CR 31 32 2E 30 0D
PDW-HIGH “18.0” 5 1 8 . 0 CR 31 38 2E 30 0D
<End> Extended data block
End of Text 0x03 1 ETX 03

No. of

Transferred Data in Characters Transferred Data in Hexadecimal

Byte

... CR … … 20 20 20 20 20 20 … … 0D

4

• “0x0D” is at the end of each item as a separating character but there is a comma “,” at the end of primary type, primary
format version, RDW-SD, IG and IG%.

• Each item is entered by ASCII code. Only “Start of Text” and “ End of Text” are entered by control code.

• Each item is initialized at space (0x20). Unused items are also initialized at space and have “0x0D” in the end.

Operator’s Manual MEK-7300K 4.9

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Transfer Format PC (V05-01)

Transfer Example to a PC

Common data block “_” in the Transferred Data in Characters column indicates a blank (0x20).

No. of

Transfer Items Example

<Start> Common data block
Start of Text 0x02
Hematology analyzer information
Type “MEK-7300”
Parameter no “23”
Send data bytes “01024”
Sampling mode “MANUAL”
Parameter “CBC + Diff”
Sample code “01”

Sample label “BLOOD”

Rack location “MMM”
Seq# “0000001”
Software version “V01-01”

Analysis program version

Format version “V05-01”
Total data bytes “02048”
Data block pattern “2”
Reserve data
Measurement data

Date “20050101”

Time “153000”
ID “ABCDEFGH:0001”
WBC “6.2”
NE% “70.6”
LY% “21.2”
MO% “2.5”
EO% “5.4H”
BA% “0.3”
NE “4.4”
LY “1.3”
MO “0.2”
EO “0.2”
BA “0.0”
RBC “5.10”
HGB “14.4”
HCT “42.3”
MCV “86.2”
MCH “28.5”
MCHC “33.1”
RDW-CV “11.5”
PLT “280”
PCT “0.15”
MPV “7.2”
PDW “18.5”
Reserve data
Flag data (with flag +, without flag (space))
Leukocytosis “+”
Leukopenia “+”
Neutrophilia “+”
Neutropenia “+”
Lymphocytosis “+”
Lymphopenia “+”
Monocytosis “+”
Eosinophilia “+”
Basophilia “+”
Blasts “+”

Immature Granulocyte

Left Shift “+”

Atypical lymphocytes

Poor hemolyzation “+”

Small nucleated cell

Ly-Mo interference “+”
Ne-Eo interference “+”
Reserve data
Erythrocytosis “+”
Anemia “+”
Anisocytosis “+”
Microcytosis “+”
Macrocytosis “+”
Hypochromia “+”
Abnormal MCHC “+”
Reserve data
Thrombocytosis “+”
Thrombocytopenia “+”
PLT Clumps “+”

PLT-RBC interference

Reserve data
Reserve data
Reserve data
<End> Common data block
End of Text 0x03

“V04-02”

“+”

“+”

“+”

“+”

Transferred Data in Characters Transferred Data in Hexadecimal

Byte

1 STX 02

11 M E K - 7 3 0 0 CR 4D 45 4B 2D 37 33 30 30 20 20 0D

6 2 3 CR 20 20 20 32 32 0D

6 0 1 0 2 4 CR 30 31 30 32 34 0D
13 M A N U A L CR 4D 41 4E 55 41 4C 20 20 20 20 20 20 0D
13 C B C + D i f f CR 43 42 43 20 2B 20 44 69 66 66 20 20 0D

3 0 1 CR 30 31 0D

17 B L O O D CR

5 M M M CR 4D 4D 4D 20 0D
11 0 0 0 0 0 0 1 CR 30 30 30 30 30 30 31 20 20 20 0D

9 V 0 1

9 V 0 4

9 V 0 5

6 0 2 0

6 2 CR 32 20 20 20 20 0D

4 × 3 Byte + CR × 1 Byte 20 × 3 Byte + 0D × 1 Byte

17 2 0 0 5 CR 0 1 CR 0 1 CR CR

9 1 5 CR 3 0 CR 0 0 CR 31 35 0D 33 30 0D 30 30 0D
16 A B C D E F G H : 0 0 0 1 CR

7 6 . 2 CR 20 36 2E 32 20 20 0D

7 7 0 . 6 CR 37 30 2E 36 20 20 0D

7 2 1 . 2 CR 32 31 2E 32 20 20 0D

7 2 . 5 CR 20 32 2E 35 20 20 0D

7 5 . 4 H CR 20 35 2E 34 48 20 0D

7 0 . 3 CR 20 30 2E 33 20 20 0D

7 4 . 4 CR 20 34 2E 34 20 20 0D

7 1 . 3 CR 20 31 2E 33 20 20 0D

7 0 . 2 CR 20 30 2E 32 20 20 0D

7 0 . 2 CR 20 30 2E 32 20 20 0D

7 0 . 0 CR 20 30 2E 30 20 20 0D

7 5 . 1 0 CR 35 2E 31 30 20 20 0D

7 1 4 . 4 CR 31 34 2E 34 20 20 0D

7 4 2 . 3 CR 34 32 2E 33 20 20 0D

7 8 6 . 2 CR 38 36 2E 32 20 20 0D

7 2 8 . 5 CR 32 38 2E 35 20 20 0D

7 3 3 . 1 CR 33 33 2E 31 20 20 0D

7 1 1 . 5 CR 31 31 2E 35 20 20 0D

7 2 8 0 CR 20 32 38 30 20 20 0D

7 0 . 1 5 CR 30 2E 31 35 20 20 0D

7 7 . 2 CR 20 37 2E 32 20 20 0D

7 1 8 . 5 CR 31 38 2E 35 20 20 0D

210 SP × 209 Byte + CR × 1 Byte 20 × 209 Byte + 0D × 1 Byte

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D
14 SP × 13 Byte + CR × 1 Byte 20 × 13 Byte + 0D × 1 Byte

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D
10 SP × 9 Byte + CR × 1 Byte 20 × 9 Byte + 0D × 1 Byte

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

2 + CR 2B 0D

8 SP × 7 Byte + CR × 1 Byte 20 × 7 Byte + 0D × 1 Byte

400 SP × 399 Byte + CR × 1 Byte 20 × 399 Byte + 0D × 1 Byte

1 ETX 03

0 1 CR 56 30 31 2D 30 31 20 20 0D

­0 2 CR 56 30 34 2D 30 32 20 20 0D

­0 1 CR 56 30 35 2D 30 31 20 20 0D

­8 CR 30 32 30 34 38 0D

4

42 4C 4F 4F 44 20 20 20 20 20 20 20 20 20 20
20 0D

32 30 30 35 0D 30 31 0D 30 31 0D 20 20 20 20
20 0D

41 42 43 44 45 46 47 48 3A 30 30 30 31 20 20 0D

(ex. 20 0D)

4.10 Operator’s Manual MEK-7300K

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Extended data block “_” in the Transferred Data in Characters column indicates a blank (0x20).

Transfer Items Example

<Start> Extended data block
Start of Text 0x02 1 STX 02
Hematology analyzer information
Identifier “EXI” 4 E X I CR 45 58 50 0D
Send data bytes “00512” 6 0 0 5 1 2 CR 30 30 35 31 32 0D
Type “MEK-7300” 11 M E K - 7 3 0 0 CR 4D 45 4B 2D 37 32 32 32 20 20 0D
Unit no “0” 3 0 CR 20 31 0D
Work list data
Name “DAVID” 27 D A V I D ... … CR 44 41 56 49 44 … … 20 20 20 20 … … 0D
Sex “MALE” 7 M A L E CR 4D 41 4C 45 20 20 0D
Date of birth “19800219” 11 1 9 8 0 CR 0 2 CR 1 9 CR 31 39 38 30 0D 30 32 0D 31 39 0D
Age “22” 4 2 2 CR 20 32 32 0D
Department “INTERNAL” 14 I N T E R N A L CR 49 4E 54 45 52 4E 41 4C 20 20 20 20 20 0D

Physician “WATSON” 27 W A T S O N ... ... CR 57 57 41 54 53 4F … … 20 20 20 … … 0D

Operator name “” 9 CR 20 20 20 20 20 20 20 20 0D

Comments “No problem.” 129 N o p r o b l e m . ... 4E 4E 6F 20 70 72 6F 62 6C 65 6D

Normal range table no“0” 2 0 CR 30 0D
Work list flag “1” 2 1 CR 31 0D
Control mode flag “” 2 CR 20 0D
Reserve data for work list data 32 SP × 31 Byte + CR × 1 Byte 20 × 31 Byte + 0D × 1 Byte
Normal range setting data
WBC-LOW “4.0” 5 4 . 0 CR 20 34 2E 30 0D
WBC-HIGH “9.0” 5 9 . 0 CR 20 39 2E 30 0D
NE%-LOW “42.0” 5 4 2 . 0 CR 34 32 2E 30 0D
NE%-HIGH “85.0” 5 8 5 . 0 CR 38 35 2E 30 0D
LY%-LOW “11.0” 5 1 1 . 0 CR 31 31 2E 30 0D
LY%-HIGH “49.0” 5 4 9 . 0 CR 34 39 2E 30 0D
MO%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
MO%-HIGH “9.0” 5 9 . 0 CR 20 39 2E 30 0D
EO%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
EO%-HIGH “3.0” 5 3 . 0 CR 20 33 2E 30 0D
BA%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
BA%-HIGH “2.0” 5 2 . 0 CR 20 32 2E 30 0D
NE-LOW “1.7” 5 1 . 7 CR 20 31 2E 37 0D
NE-HIGH “7.7” 5 7 . 7 CR 20 37 2E 37 0D
LY-LOW “0.4” 5 0 . 4 CR 20 30 2E 34 0D
LY-HIGH “4.4” 5 4 . 4 CR 20 34 2E 34 0D
MO-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
MO-HIGH “0.8” 5 0 . 8 CR 20 30 2E 38 0D
EO-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
EO-HIGH “0.3” 5 0 . 3 CR 20 30 2E 33 0D
BA-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
BA-HIGH “0.2” 5 0 . 2 CR 20 30 2E 32 0D
RBC-LOW “3.80” 5 3 . 8 0 CR 33 2E 38 30 0D
RBC-HIGH “5.30” 5 5 . 3 0 CR 35 2E 33 30 0D
HGB-LOW “11.0” 5 1 1 . 0 CR 31 31 2E 30 0D
HGB-HIGH “17.0” 5 1 7 . 0 CR 31 37 2E 30 0D
HCT-LOW “36.0” 5 3 6 . 0 CR 33 36 2E 30 0D
HCT-HIGH “56.0” 5 5 6 . 0 CR 35 36 2E 30 0D
MCV-LOW “80.0” 5 8 0 . 0 CR 38 30 2E 30 0D
MCV-HIGH “100” 5 1 0 0 CR 20 31 30 30 0D
MCH-LOW “28.0” 5 2 8 . 0 CR 32 38 2E 30 0D
MCH-HIGH “36.0” 5 3 6 . 0 CR 33 36 2E 30 0D
MCHC-LOW “31.0” 5 3 1 . 0 CR 33 31 2E 30 0D
MCHC-HIGH “37.0” 5 3 7 . 0 CR 33 37 2E 30 0D
RDW-CV-LOW “11.5” 5 1 1 . 5 CR 31 31 2E 35 0D
RDW-CV-HIGH “16.5” 5 1 6 . 5 CR 31 36 2E 35 0D
PLT-LOW “120” 5 1 2 0 CR 20 31 32 30 0D
PLT-HIGH “380” 5 3 8 0 CR 20 33 38 30 0D
PCT-LOW “0.10” 5 0 . 1 0 CR 30 2E 31 30 0D
PCT-HIGH “1.00” 5 1 . 0 0 CR 31 2E 30 30 0D
MPV-LOW “5.0” 5 5 . 0 CR 20 35 2E 30 0D
MPV-HIGH “10.0” 5 1 0 . 0 CR 31 30 2E 30 0D
PDW-LOW “12.0” 5 1 2 . 0 CR 31 32 2E 30 0D
PDW-HIGH “18.0” 5 1 8 . 0 CR 31 38 2E 30 0D
<End> Extended data block
End of Text 0x03 1 ETX 03

No. of

Transferred Data in Characters Transferred Data in Hexadecimal

Byte

... CR … … 20 20 20 20 20 20 … … 0D

4

Operator’s Manual MEK-7300K 4.11

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

Extended data block 2

Transfer Items Example

MEK-7300 extended data
<Start> Extended data block
Start of Text 0x02 1 STX 02
Hematology analyzer information
Identifier “EX2” 4 E X 2 CR 45 58 32 0D
Send data bytes “00512” 6 0 0 5 1 2 CR 30 30 35 31 32 0D
Type “MEK-7300” 11 M E K - 7 3 0 0 CR 4D 45 4B 2D 37 33 30 30 20 20 0D
Serial no “1234567” 8 1 2 3 4 5 6 7 CR 31 32 33 34 35 36 37 0D
Reserve data 49 × 48 Byte + CR × 1 Byte 20 × 48 Byte + 0D × 1 Byte
Measurement data

Patient id “12345” 17 1 2 3 4 5 CR

Reserve data 48 × 47 Byte + CR × 1 Byte 20 × 47 Byte + 0D × 1 Byte
RDW-SD 40.3 7 4 0 . 3 CR 34 30 2E 33 20 20 0D
IG 0.2 7 0 . 2 CR 20 30 2E 32 20 20 0D
IG% 1.3 7 1 . 3 CR 20 31 2E 33 20 20 0D
Reserve data 56 × 55 Byte + CR × 1 Byte 20 × 55 Byte + 0D × 1 Byte
Normal range data
RDW-SD-LOW “39.0” 5 3 9 . 0 CR 33 39 2E 30 0D
RDW-SD-HIGH “46.0” 5 4 6 . 0 CR 34 36 2E 30 0D
Reserve data 1 “” 5 CR 20 20 20 20 0D
Reserve data 2 “” 5 CR 20 20 20 20 0D
Reserve data 3 “” 5 CR 20 20 20 20 0D
Reserve data 4 “” 5 CR 20 20 20 20 0D
Reserve data 80 × 79 Byte + CR × 1 Byte 20 × 79 Byte + 0D × 1 Byte
Unit data
WBC “×10^2/uL” 12 × 1 0 ^ 2 / u L CR 78 31 30 5E 32 2F 75 4C 20 20 20 0D
RBC “×10^4/uL” 12 × 1 0 ^ 4 / u L CR 78 31 30 5E 34 2F 75 4C 20 20 20 0D
HGB “g/dL” 12 g / d L CR 67 2F 64 4C 20 20 20 20 20 20 20 0D
HCT “L/L” 12 L / L CR 4C 2F 4C 20 20 20 20 20 20 20 20 0D
PLT “×10^4/uL” 12 × 1 0 ^ 4 / u L CR 78 31 30 5E 34 2F 75 4C 20 20 20 0D
RDW-CV “%CV” 12 % C V CR 25 43 56 20 20 20 20 20 20 20 20 0D
PDW “%” 12 % CR 25 20 20 20 20 20 20 20 20 20 20 0D
Reserve data 96 × 95 Byte + CR × 1 Byte 20 × 95 Byte + 0D × 1 Byte
<End> Extended data block
End of Text 0x03 1 ETX 03

No. of

Byte

Total 512

Transferred Data in Characters Transferred Data in Hexadecimal

31 32 33 34 35 20 20 20 20 20 20 20 20 20
20 20 0D

• “0x0D” is at the end of each item as separating character.

• Each item is entered by ASCII code. Only “Start of Text” and “ End of Text” are entered by control code.

• Each item is initialized at space (0x20). Unused items are also initialized at space and have “0x0D” in the end.

• For the communication, consult your Nihon Kohden representative.

4.12 Operator’s Manual MEK-7300K

ASTM Communication

The communication specications of the analyzer are based on the OSI
Reference Model (ISO7498) and comply with the following ASTM standards.

Layer OSI Reference Model Applicable Standard

For connection between the analyzer and a receiver, use the serial port and an
RS-232C cable. In addition, set the <External device> setting on the Serial Port
Setting to “ASTM”. For the setting, refer to “Changing Serial Port Settings” in
Section 5.

For details on the ASTM communication specications, contact your Nihon
Kohden representative.

4. PERFORMANCE CHARACTERISTICS AND SPECIFICATIONS

6 Presentation layer
2 Data link layer ASTM E1381-02
1 Physical layer ASTM E1381-02

ASTM E1394-97

4

Operator’s Manual MEK-7300K 4.13

Section 5 Operating Instructions

Screen Configuration ........................................................................................................................................... 5.4

Flowchart of Screens ................................................................................................................................ 5.4

Ready Screen ............................................................................................................................................ 5.6

Work List Screen .......................................................................................................................................5.6

Data Screen ..............................................................................................................................................5.7

Quality Control Screen .............................................................................................................................. 5.8

Calibration Screen ..................................................................................................................................... 5.8

Operation Screen ...................................................................................................................................... 5.9

System Screen ..........................................................................................................................................5.9

Changing Settings ............................................................................................................................................. 5.10

General .................................................................................................................................................... 5.10

System Screen ........................................................................................................................................5.10

Assigning Users and Passwords ...................................................................................................5.10

Doing Maintenance ....................................................................................................................... 5.16

Checking the Error Log ................................................................................................................. 5.22

Performing an Optical Adjustment ................................................................................................. 5.23

Settings Screen .......................................................................................................................................5.34

Displaying Settings Screen. .......................................................................................................... 5.34

Labeling and Selecting Sample Types .......................................................................................... 5.35

Setting Normal Range Upper and Lower Limits ............................................................................ 5.38

Changing the Limits ......................................................................................................................5.39

Selecting Units .............................................................................................................................. 5.40

Operation Settings ........................................................................................................................5.42

Flag Settings ................................................................................................................................. 5.45

QC Settings Window .....................................................................................................................5.49

Setting Reagent Management ......................................................................................................5.50

Setting Auto Priming/Cleaning ...................................................................................................... 5.55

Setting Date and Time...................................................................................................................5.57

Synchronizing the Date and Time .................................................................................................5.59

Changing Output Format ............................................................................................................... 5.60

Formatting SD Card ...................................................................................................................... 5.72

Changing Sound and LCD Settings .............................................................................................. 5.73

Initializing the Analyzer .................................................................................................................5.74

Interference Substances .................................................................................................................................... 5.76

Measurement ..................................................................................................................................................... 5.78

General .................................................................................................................................................... 5.78

Open and Closed Sampling Modes ..............................................................................................5.78

Dilution Modes ..............................................................................................................................5.78

Sample Types ................................................................................................................................5.79

Single and Double Counting .........................................................................................................5.79

ID Number ..................................................................................................................................... 5.80

Alarm Display ................................................................................................................................5.80

Data Storage .................................................................................................................................5.80

5

Operator’s Manual MEK-7300K 5.1

Printing and Sending Data ............................................................................................................ 5.80

Advanced Count ............................................................................................................................ 5.81

Counting Special Case Samples ................................................................................................... 5.81

Assigning an ID to a Sample and Patient ................................................................................................ 5.81

Not Assigning an ID ......................................................................................................................5.82

Using Bar Codes ...........................................................................................................................5.83

Measurement in Closed Mode ................................................................................................................5.83

Preparing a Sample ......................................................................................................................5.83

Measuring a Sample in Closed Mode ........................................................................................... 5.85

Measurement in Open Mode ................................................................................................................... 5.89

Measuring a Venous Sample in Normal Dilution Mode .................................................................5.89

Measuring a Pre-Dilution Sample .................................................................................................5.92

Measuring a Venous Sample in WBC Low/High Dilution Mode .................................................... 5.96

Measuring a Sample in Capillary Mode .................................................................................................. 5.98

Preparation .................................................................................................................................... 5.98

Measurement ................................................................................................................................ 5.99

Auto Recount ......................................................................................................................................... 5.100

Recount on Alarm .......................................................................................................................5.100

Recount on PLT Count ................................................................................................................5.100

Work Lists ........................................................................................................................................................ 5.101

General .................................................................................................................................................. 5.101

Entering New Work List Data ................................................................................................................ 5.101

Receiving Work List Data ......................................................................................................................5.104

Editing Work List Data ........................................................................................................................... 5.105

Searching for Work List Data ................................................................................................................. 5.106

Deleting Work List Data .........................................................................................................................5.108

To Delete One Data .....................................................................................................................5.108

To Delete Several Consecutive Data ........................................................................................... 5.108

Measuring a Sample Using the Work List ............................................................................................. 5.110

Description of the Results Screen ................................................................................................................... 5.111

Enlarging Measurement Results .................................................................................................5.111

Large Scattergram ......................................................................................................................5.112

Large Histogram .......................................................................................................................... 5.112

Measuring Units .......................................................................................................................... 5.113

Alarm Display ........................................................................................................................................ 5.113

Printing and Sending Results ................................................................................................................5.114

Auto Printing/Sending after Measurement .................................................................................. 5.114

Printing by Pressing [Print] Key ................................................................................................... 5.114

Sending Data to PC ....................................................................................................................5.114

Handling Data .................................................................................................................................................. 5.115

General .................................................................................................................................................. 5.115

Displaying Saved Data ..........................................................................................................................5.115

Editing the ID of Saved Data .................................................................................................................5.117

Searching for Saved Data ..................................................................................................................... 5.118

Printing and Sending Saved Data ......................................................................................................... 5.124

Automatic Printing and Sending Data after Measurement ..........................................................5.124

Printing and Sending Stored Data............................................................................................... 5.124

RS-232C Data Transfer .........................................................................................................................5.128

Deleting Saved Data..............................................................................................................................5.129

5.2 Operator’s Manual MEK-7300K

Deleting One Data ....................................................................................................................... 5.129

Deleting Multiple Data .................................................................................................................5.130

Deleting All Stored Data .............................................................................................................. 5.131

Turning the Power Off ...................................................................................................................................... 5.132

Daily Shutdown .....................................................................................................................................5.132

Checking List Before Turning the Power Off ................................................................................5.132

Turning the Power Off .................................................................................................................. 5.132

Check List After Turning Power Off .............................................................................................5.132

Check List Before Long Term Storage......................................................................................... 5.133

Auto Cleaning and Priming when Operating All Night ................................................................. 5.133

Strong Cleaning ............................................................................................................................................... 5.134

Draining the Measurement Baths and Sub Baths ...........................................................................................5.136

5

Operator’s Manual MEK-7300K 5.3

5. OPERATING INSTRUCTIONS

Ready

Screen Configuration

Flowchart of Screens

Power ON

Checking

Ready

Work list

Memory

Search

Data

Memory

SD Card

Daily

Search

QC (QUALITY CONTROL)

X-R

L&J

Measurement

Search

ID
Date
Sample type

Flag

Measure

History

Graph

Vial mgnt

Measure

History

Graph

Vial mgnt

Results

Output

XB

Data list

Graph

Setting

Calibration

Setting

History

Data list

5.4 Operator’s Manual MEK-7300K

Operations

Refill all

Replace dil
Replace lys.
Replace det

Strong clean
Clean flowcell
Clean baths

Remove clog

Drain all

Drain baths
Circuit Check
Background

System

Users
User Maint.

Error log

Adj. Flowcell

Settings

5. OPERATING INSTRUCTIONS

5

Sample type
Normal range
Units

Operation

Flags

QC Settings

Reagent mgmt

Auto Clean

Date & time

Output

Formatting SD card

Sound/LCD
Initialize

Service (This screen is for service personnel when performing maintenance.)

Operator’s Manual MEK-7300K 5.5

5. OPERATING INSTRUCTIONS

Ready Screen

The Ready screen is displayed when the analyzer is powered on and after
cleaning and priming.

On the Ready screen, you can immediately start measurement after checking
measuring accuracy and setting various necessary settings. You can set the
sample ID and select open mode or closed mode.

Work List Screen

On this screen, you can manage work lists or start measurement from a work list.

By registering work list, the analyzer can set measurement parameters for each
sample or attach patient information to the measurement data. Up to 50 work
lists can be registered. Refer to “Using Work Lists” in this section.

5.6 Operator’s Manual MEK-7300K

Data Screen

5. OPERATING INSTRUCTIONS

On the Data screen, you can display measured results. Histograms of a measured
sample can be displayed by pressing the desired data and pressing the Detail key.
You can delete, print or transfer data to a personal computer. Refer to “Handling
Data” in this section.

5

Data-List screen

Data-Details screen

Operator’s Manual MEK-7300K 5.7

5. OPERATING INSTRUCTIONS

Quality Control Screen

Quality control refers to the precision, accuracy and reproducibility in the
system. The analyzer provides four programs for quality control. Refer to Section
11 “Quality Control”.

Calibration Screen

The calibration coefcient is used in converting the data to the measured values
to make the measured values as close as possible to the real values. Calibration
can be performed in venous blood and pre-diluted blood individually. Refer to
Section 6 “Calibration Procedures”.

5.8 Operator’s Manual MEK-7300K