Nihon-Kohden Celltak MEK-6400 Service manual

Automated

Hematology Analyzer

MEK-6400/MEK-6410/MEK-6420

MEK-6400C MEK-6410C MEK-6400J MEK-6410J MEK-6400K MEK-6410K

MEK-6420C MEK-6420J MEK-6420K

If you have any comments or suggestions
on this manual, please contact us at:	0634-900086B
www.nihonkohden.com

		CONTENTS
	Contents
	GENERAL HANDLING PRECAUTIONS ..............................................................................	i
	WARRANTY POLICY .........................................................................................................	ii
	RESPONSIBILITIES – PROFESSIONAL USERS .............................................................	ii
	EMC Related Caution ........................................................................................................	iii
	Conventions Used in this Manual and Instrument .............................................................	v
	Warnings, Cautions and Notes ................................................................................	v
Section 1	General .................................................................................	1.1
	Introduction ....................................................................................................................	1.2
	Service Policy ................................................................................................................	1.3
	Specifications ................................................................................................................	1.4
	Measured Parameters, Ranges and Reproducibility to Specimen from Venous
	Blood ..........................................................................................................	1.4
	Standardization Analysis Method ...............................................................	1.4
	Detection Method .......................................................................................	1.5
	Dilution Ratio ..............................................................................................	1.5
	Counting Time ............................................................................................	1.5
	Display .......................................................................................................	1.5
	Data Storage ..............................................................................................	1.5
	Environmental Conditions ...........................................................................	1.5
	Power Requirements ..................................................................................	1.5
	Dimensions and Weight ..............................................................................	1.6
	Electromagnetic Compatibility ....................................................................	1.6
	Safety ........................................................................................................	1.6
	Panel Description ...........................................................................................................	1.7
	Front Panel ...........................................................................................................	1.7
	Right Side Panel ...................................................................................................	1.8
	Rear Panel ...........................................................................................................	1.9
	Composition ..................................................................................................................	1.10
	MEK-6400 ...........................................................................................................	1.10
	MEK-6410 ...........................................................................................................	1.11
	MEK-6420 ...........................................................................................................	1.12
	Interference Substances ...............................................................................................	1.13
Section 2	Troubleshooting ..................................................................	2.1
	Check Procedure Flowchart ...........................................................................................	2.4
	Operating Condition and Blood Sample Checks .............................................................	2.5
	Operating Condition Check ...................................................................................	2.5
	Blood Sample Handling Check .............................................................................	2.5
	Pre-dilution Sample Preparation Check ................................................................	2.5
	Checking the Hematology Analyzer ................................................................................	2.7
	Checking Background Noise ................................................................................	2.7
	Measurement .............................................................................................	2.7

Service Manual MEK-6400/6410/6420

C.1

CONTENTS

Parameter Data Check with Diluent	............................................................ 2.7
Reducing Background Noise ......................................................................	2.8
Background Noise Problems ......................................................................	2.9
Checking the Reproducibility ................................................................................	2.9
Checking Procedure ...................................................................................	2.9
CV Values .................................................................................................	2.10
Checking Accuracy .......................................................................................................	2.11
Checking Procedure ............................................................................................	2.11
Poor WBC Reproducibility ....................................................................................	2.11
Poor HGB Reproducibility ....................................................................................	2.12
Poor RBC Reproducibility ....................................................................................	2.13
Poor PLT Reproducibility .....................................................................................	2.14
Poor HCT or MCV Reproducibility .......................................................................	2.15
Solving Problems from Alarm Messages ......................................................................	2.16
A001: No diluent ........................................................................................	2.16
A005: No detergent ...................................................................................	2.16
A007: No lysing reagent ............................................................................	2.17
A009: WBC priming error ...........................................................................	2.17
A010: RBC PRIMING ERROR ...................................................................	2.17
A021: WBC level 1 ....................................................................................	2.17
A022: WBC level 2 ....................................................................................	2.18
A023: WBC level 3 ....................................................................................	2.18
A024: WBC bubble 1 .................................................................................	2.18
A025: WBC bubble 2 .................................................................................	2.18
A026: WBC bubble 3 .................................................................................	2.19
A027: WBC bubble 4 .................................................................................	2.19
A029: WBC clog ........................................................................................	2.19
A030: WBC sample error ...........................................................................	2.19
A031: WBC noise 2 ...................................................................................	2.20
A032: WBC noise 1 ...................................................................................	2.20
A036: WBC upper manometer dirty ...........................................................	2.20
A037: WBC lower manometer dirty ............................................................	2.20
A041: RBC level 1 .....................................................................................	2.21
A042: RBC level 2 .....................................................................................	2.21
A043: RBC level 3 .....................................................................................	2.21
A044: RBC bubble 1 ..................................................................................	2.22
A045: RBC bubble 2 ..................................................................................	2.22
A046: RBC bubble 3 ..................................................................................	2.22
A047: RBC bubble 4 ..................................................................................	2.22
A049: RBC clog .........................................................................................	2.22
A050: RBC sample error ............................................................................	2.23
A051: RBC noise 2 ....................................................................................	2.23
A052: PLT noise 1 .....................................................................................	2.23
A053: PLT noise 3 .....................................................................................	2.23
A057: RBC upper manometer dirty ............................................................	2.23
A058: RBC lower manometer dirty ............................................................	2.23
A061: HGB voltage low .............................................................................	2.24
A062: HGB voltage high ............................................................................	2.24
A063: HGB circuit error .............................................................................	2.24

C.2	Service Manual MEK-6400/6410/6420

	CONTENTS
A072: Tube holder open .............................................................................	2.24
A073: Tube in the holder ............................................................................	2.25
A091: Room temperature high ...................................................................	2.25
A092: Room temperature low ....................................................................	2.25
A093: Internal temperature high ................................................................	2.25
A094: Internal temperature low ..................................................................	2.25
A095: Power supply temp high ..................................................................	2.25
! APPEARS ON THE RIGHT OF WBC MEASURED VALUE ....................	2.26
! APPEARS ON THE RIGHT OF MCHC MEASURED VALUE ..................	2.26
C APPEARS ON THE RIGHT OF WBC OR PLT MEASURED VALUE or
PLT Clumps appears .................................................................................	2.27
OTHER FLAGS .........................................................................................	2.27
Solving Problems from System Error Messages ...........................................................	2.28
E001: DILUTER INITIALIZE ERROR ........................................................	2.28
E021: SAMPLER INITIALIZE ERROR ......................................................	2.28
E041: SUB BATH INITIALIZE ERROR ......................................................	2.28
E101: BATH DRAIN ERROR .....................................................................	2.29
E122: CHECK SETTINGS ........................................................................	2.29
E123: MEMORY ERROR ..........................................................................	2.29
E124: CIRCUIT ERROR ............................................................................	2.29
E126: TOUCH PANEL ERROR ..................................................................	2.29
E141: CAP PIERCE INITIALIZE ERROR .................................................	2.29
Service Maintenance Screens .......................................................................................	2.30
Displaying the SERVICE Screen .........................................................................	2.30
CHECK MENU Screen ........................................................................................	2.32
UNIT MAINTE Screen .........................................................................................	2.32
MD-640V ...................................................................................................	2.33
MS-640V ...................................................................................................	2.33
MS-641V (MEK-6400 only) ........................................................................	2.34
MV & PUMP CHK .....................................................................................	2.34
CONT MEASURE Screen ...................................................................................	2.35
SETTING MENU Screen .....................................................................................	2.35
ADV. SETTINGS Screen ...........................................................................	2.35
REAGENT FACTOR Screen ......................................................................	2.36
USER MAINTENANCE Screen .................................................................	2.36
PANIC VALUE Screen (for MEK-6400 and MEK-6410) ..............................	2.37
FLAG SETTINGS Screen .........................................................................	2.37
BAR CODE PORT Screen ........................................................................	2.37
UNIT CAL Screen .....................................................................................	2.38
MONITOR Screen ...............................................................................................	2.38
X10-CV screen ....................................................................................................	2.39
INITIAL MENU Screen ........................................................................................	2.40
OTHER MENU Screen ........................................................................................	2.40
PRINT SETTINGS Screen ..................................................................................	2.40

Section 3 Board/Unit Description .......................................................	3.1
Board and Unit Location .................................................................................................	3.3
Right Side View ....................................................................................................	3.3

Service Manual MEK-6400/6410/6420

C.3

CONTENTS
Left Side View ......................................................................................................	3.3
Front View ............................................................................................................	3.4
Top View ...............................................................................................................	3.4
MC-640V Measuring Unit ................................................................................................	3.5
MD-640V Combination Syringe Pump Unit .....................................................................	3.5
MP-640V Pump Unit .......................................................................................................	3.6
PV-641V Front Panel Unit ...............................................................................................	3.6
JQ-640V Inlet/Outlet Unit ...............................................................................................	3.7
JQ-641V/JQ-642V Valve Unit .........................................................................................	3.7
JQ-641V Valve Unit ..............................................................................................	3.7
JQ-642V Valve Unit ..............................................................................................	3.7
MS-640V Sampler Unit ...................................................................................................	3.8
MS-641V Cap Pierce Unit (MEK-6400 only) ...................................................................	3.8
MR-640V Rinse Unit (MEK-6410/6420 only) ...................................................................	3.9
UT-7192 AMP CONTROL Board .....................................................................................	3.9
UT-7193 POWER Board ................................................................................................	3.10
UT-7198 MEASURING Board ........................................................................................	3.10
UT-7201 HGB AMP Board and UT-7202 HGB LED Board ..............................................	3.11
UT-7200 MIXED PUMP Board .......................................................................................	3.11
UT-7205 PRINTER DRIVER Board ................................................................................	3.12
UT-7203 KEY Board ......................................................................................................	3.12
UT-7199 LIQUID SENSOR Board ..................................................................................	3.13
XP-602V/XP-612V 2-way Electromagentic Valve ..........................................................	3.13

Section 4	Disassembly and Assembly ..............................................	4.1
	Before You Begin ............................................................................................................	4.2
	Warnings and Cautions .........................................................................................	4.2
	Required Tools ......................................................................................................	4.2
	Caution and Notes Related to Valve Joint, Black Screw and Tube Joint in the
	Instrument ............................................................................................................	4.3
	Board and Unit Location .......................................................................................	4.3
	Turning the Power Off .....................................................................................................	4.4
	Cleaning and Draining the Fluid Pathway ..............................................................	4.4
	Turning the Power Off ...........................................................................................	4.5
	Removing the Right Side Cover, Top Cover and Rear Cover ...........................................	4.6
	Reattaching the Right Side Cover, Top Cover and Rear Cover .............................	4.7
	Removing the Front Panel Unit .......................................................................................	4.8
	Reattaching the Front Panel Unit ..........................................................................	4.9
	Installing the WA-640VK Printer Unit (Option) ................................................................	4.10
	Removing the Measuring Unit .......................................................................................	4.12
	Reattaching the Measuring Unit ..........................................................................	4.13
	Accessing the Connectors on the POWER Board .........................................................	4.14
	Reassembling the Board .....................................................................................	4.14
	Removing the Combination Syringe Pump Unit .............................................................	4.15
	Reattaching the Combination Syringe Pump Unit ................................................	4.16
	Removing the Pump Unit ...............................................................................................	4.17
	Reattaching the Pump Unit .................................................................................	4.18
	Removing the Sampler Unit ...........................................................................................	4.19

C.4	Service Manual MEK-6400/6410/6420

		CONTENTS
	Reattaching the Sampler Unit..............................................................................	4.22
	Removing the Cap Pierce Unit or Rinse Unit .................................................................	4.23
	Reattaching the Cap Pierce Unit or Rinse Unit ....................................................	4.24
	Removing the Inlet/Outlet Unit ......................................................................................	4.25
	Reattaching the Inlet/Outlet Unit .........................................................................	4.26
	Removing the Valve Unit ...............................................................................................	4.26
	Reattaching the Valve Unit ..................................................................................	4.27
	Removing the AMP CONTROL Board ...........................................................................	4.28
	Reattaching the AMP CONTROL Board ..............................................................	4.29
	Removing the POWER Board ........................................................................................	4.30
	Reattaching the POWER Board ..........................................................................	4.31
	Removing the MEASURING Board ...............................................................................	4.32
	Reattaching the MEASURING Board ..................................................................	4.33
	Removing the MIXED PUMP Board ..............................................................................	4.34
	Reattaching the MIXED PUMP Board .................................................................	4.34
	Removing the PRINTER DRIVER Board .......................................................................	4.35
	Reattaching the PRINTER DRIVER Board ..........................................................	4.35
	Removing the KEY Board ..............................................................................................	4.36
	Reattaching the KEY Board ................................................................................	4.36
	Removing the LIQUID SENSOR Board .........................................................................	4.37
	Reattaching the LIQUID SENSOR Board ............................................................	4.37
	Removing the LCD ........................................................................................................	4.38
	Reattaching the LCD ...........................................................................................	4.39
	Removing the Transformer .............................................................................................	4.40
	Reattaching the Transformer ................................................................................	4.40
Section 5	Adjustment...........................................................................	5.1
	General ...........................................................................................................................	5.2
	Adjusting the HGB Sensor Output Voltage .....................................................................	5.3
	Adjusting the Upper and Lower Sensor Output Voltages of the Manometers ..................	5.6
	Adjusting the Liquid Sensor Output Voltages ..................................................................	5.9
Section 6	Maintenance ........................................................................	6.1
	General ...........................................................................................................................	6.3
	Disposing of Waste ...............................................................................................	6.4
	Repair Parts Availability Policy .............................................................................	6.4
	Parts to be Replaced Periodically .........................................................................	6.4
	Maintenance Schedule ...................................................................................................	6.5
	Displaying Operation History Screen ..............................................................................	6.6
	Maintenance Check Sheet .............................................................................................	6.8
	Before Maintenance Procedure .....................................................................................	6.12
	Strong Cleaning ...................................................................................................	6.12
	Draining Measurement Baths and Sub Baths ......................................................	6.13
	Turning Power Off ................................................................................................	6.14
	Daily Maintenance Procedures ......................................................................................	6.15
	Checking Reagents and Other Consumables ......................................................	6.15
	Checking the Appearance of the Analyzer ..........................................................	6.15

Service Manual MEK-6400/6410/6420

C.5

CONTENTS

Disinfecting the Surface of the Analyzer ...................................................	6.16
Cleaning the Surface of the Analyzer ........................................................	6.16
Checking the Reagents Connection Tubes ..........................................................	6.16
Checking the Power Cord and Grounding Lead ....................................................	6.16
Checking the External Instrument Connection ....................................................	6.16
Checking the Power On .......................................................................................	6.17
Calibrating the Touch Screen .....................................................................	6.17
Checking the Date and Time ...............................................................................	6.17
Clock Accuracy .........................................................................................	6.18
Measuring Background Noise ..............................................................................	6.18
Measuring Hematology Control ...........................................................................	6.20
Checking Measurement Baths and Sub Baths ....................................................	6.21
Checking Pump Tube ...........................................................................................	6.21
Weekly/Every 300 Counts Maintenance Procedures .....................................................	6.21
Checking/Cleaning Filters ...................................................................................	6.21
Monthly/Every 1,000 Counts Maintenance Procedures .................................................	6.22
Replacing Filters .................................................................................................	6.22
Materials Required ....................................................................................	6.22
Procedure .................................................................................................	6.22
Checking and Cleaning Measurement Baths and Sub Baths ...............................	6.23
Materials Required ....................................................................................	6.23
Procedure .................................................................................................	6.24
Checking and Cleaning/Replacing the Rinse Unit and Cap Pierce Nozzle ...........	6.26
Materials Required ....................................................................................	6.26
Procedure .................................................................................................	6.27
Every Four Months/Every 3,000 Counts Maintenance Procedures ...............................	6.29
Checking and Cleaning/Replacing the Sampling Nozzles ....................................	6.29
Materials Required ....................................................................................	6.29
Procedure .................................................................................................	6.29
Replacing Pump Tube ..........................................................................................	6.32
Materials Required ....................................................................................	6.32
Procedure .................................................................................................	6.32
Every Six Months/As-Required Maintenance Procedures .............................................	6.35
Removing a Clog from the Aperture ....................................................................	6.35
Cleaning Aperture Caps ......................................................................................	6.36
Materials Required ....................................................................................	6.36
Procedures................................................................................................	6.36
Cleaning the External Electrodes on the Measurement Baths ............................	6.39
Checking the Sensor Monitor Screen ..................................................................	6.40
Checking the Circuit ............................................................................................	6.41
_
Checking the X-R Values .....................................................................................	6.43
Checking the Current Calibration Coefficients .....................................................	6.43
Checking the New Calibration Coefficients ..........................................................	6.43
Checking the Prime Function ..............................................................................	6.44
Checking the Drain Function ...............................................................................	6.44
Checking the Cleaning Function ..........................................................................	6.44
Checking the Dispensing Function ......................................................................	6.45
Checking the External Instruments Function ......................................................	6.45
Printers .....................................................................................................	6.45

C.6	Service Manual MEK-6400/6410/6420

	CONTENTS
Hand-held Bar Code Reader ......................................................................	6.45
PC .............................................................................................................	6.45
Checking the Power Cord ....................................................................................	6.45
Checking the Resistance of the Protective Ground Line .....................................	6.45
Checking the Earth Leakage Current ...................................................................	6.46

Section 7 Test Point, Variable Resistor, LED and Switch on Board 7.1

	AMP CONTROL Board ...................................................................................................	7.2
	POWER Board ...............................................................................................................	7.4
	MEASURING Board .......................................................................................................	7.5
	HGB AMP Board ............................................................................................................	7.6
	LIQUID SENSOR Board .................................................................................................	7.6
	MIXED PUMP Board ......................................................................................................	7.7
	KEY Board .....................................................................................................................	7.7
	PRINTER DRIVER Board ...............................................................................................	7.8
Section 8	Socket Pin Assignment......................................................	8.1
	ZK-820V Bar Code Reader Socket .......................................................................	8.2
	Serial Port 1/Serial Port 2 ....................................................................................	8.2
	Printer Socket ......................................................................................................	8.2
	USB Socket .........................................................................................................	8.2
Section 9	Installation............................................................................	9.1
	Connecting the Power Cord and Grounding the Analyzer ................................................	9.2
	Connecting the Power Cord ..................................................................................	9.2
	Equipotential Grounding .......................................................................................	9.2
	Connecting Tubes and Installing Reagents .....................................................................	9.3
	Materials Required ...............................................................................................	9.3
	Connecting Tubes .................................................................................................	9.4
	Diluent Tube ...............................................................................................	9.4
	Detergent Tube ...........................................................................................	9.5
	Lysing Reagent Tube ..................................................................................	9.6
	Waste Fluid Tube ........................................................................................	9.6
	Turning Power On/Off .....................................................................................................	9.7
	Turning On the Power ...........................................................................................	9.7
	Cleaning the Fluid Path After Turning the Power On (PRIME ON INSTALL) ..........	9.8
	Turning Off the Power ...........................................................................................	9.9
	Checking Accuracy .......................................................................................................	9.10
	Checking the Circuit ............................................................................................	9.10
	Measuring Background Noise..............................................................................	9.10
	Calibrating ...........................................................................................................	9.11

Service Manual MEK-6400/6410/6420

C.7

GENERAL HANDLING PRECAUTIONS

This device is intended for use only by qualified medical personnel.

Use only Nihon Kohden approved products with this device. Use of non-approved products or in a non-approved manner may affect the performance specifications of the device. This includes, but is not limited to, batteries, recording paper, pens, extension cables, electrode leads, input boxes and AC power.

Please read these precautions thoroughly before attempting to operate the instrument.

1.To safely and effectively use the instrument, its operation must be fully understood.

2.When installing or storing the instrument, take the following precautions:

(1)Avoid moisture or contact with water, extreme atmospheric pressure, excessive humidity and temperatures, poorly ventilated areas, and dust, saline or sulphuric air.

(2)Place the instrument on an even, level floor. Avoid vibration and mechanical shock, even during transport.

(3)Avoid placing in an area where chemicals are stored or where there is danger of gas leakage.

(4)The power line source to be applied to the instrument must correspond in frequency and voltage to product specifications, and have sufficient current capacity.

(5)Choose a room where a proper grounding facility is available.

3.Before Operation

(1)Check that the instrument is in perfect operating order.

(2)Check that the instrument is grounded properly.

(3)Check that all cords are connected properly.

(4)Pay extra attention when the instrument is in combination with other instruments to avoid misdiagnosis or other problems.

(5)All circuitry used for direct patient connection must be doubly checked.

(6)Check that battery level is acceptable and battery condition is good when using battery-operated models.

4.During Operation

(1)Both the instrument and the patient must receive continual, careful attention.

(2)Turn power off or remove electrodes and/or transducers when necessary to assure the patient’s safety.

(3)Avoid direct contact between the instrument housing and the patient.

5.To Shutdown After Use

(1)Turn power off with all controls returned to their original positions.

(2)Remove the cords gently; do not use force to remove them.

(3)Clean the instrument together with all accessories for their next use.

6.The instrument must receive expert, professional attention for maintenance and repairs. When the instrument is not functioning properly, it should be clearly marked to avoid operation while it is out of order.

7.The instrument must not be altered or modified in any way.

8.Maintenance and Inspection

(1)The instrument and parts must undergo regular maintenance inspection at least every 6 months.

(2)If stored for extended periods without being used, make sure prior to operation that the instrument is in perfect operating condition.

Service Manual MEK-6400/6410/6420

i

9.When the instrument is used with an electrosurgical instrument, pay careful attention to the application and/or location of electrodes and/or transducers to avoid possible burn to the patient.

10.When the instrument is used with a defibrillator, make sure that the instrument is protected against defibrillator discharge. If not, remove patient cables and/or transducers from the instrument to avoid possible damage.

WARRANTY POLICY

Nihon Kohden Corporation (NKC) shall warrant its products against all defects in materials and workmanship for one year from the date of delivery. However, consumable materials such as recording paper, ink, stylus and battery are excluded from the warranty.

NKC or its authorized agents will repair or replace any products which prove to be defective during the warranty period, provided these products are used as prescribed by the operating instructions given in the operator’s and service manuals.

No other party is authorized to make any warranty or assume liability for NKC’s products. NKC will not recognize any other warranty, either implied or in writing. In addition, service, technical modification or any other product change performed by someone other than NKC or its authorized agents without prior consent of NKC may be cause for voiding this warranty.

Defective products or parts must be returned to NKC or its authorized agents, along with an explanation of the failure. Shipping costs must be pre-paid.

This warranty does not apply to products that have been modified, disassembled, reinstalled or repaired without Nihon Kohden approval or which have been subjected to neglect or accident, damage due to accident, fire, lightning, vandalism, water or other casualty, improper installation or application, or on which the original identification marks have been removed.

In the USA and Canada other warranty policies may apply.

RESPONSIBILITIES – PROFESSIONAL USERS

This instrument must be used by a professional user with a full knowledge of operating this instrument, only for his/her intended use and according to the instructions for use. Instructions in the operator’s manual must be followed, especially the following points.

•Storage and stability of reagents

•Handling of reagents

•Instrument installation

•Connection of all tubes to inlets and outlets

•Connection of all tubes to reagents and waste container

•Checking the amount of reagents and waste fluid

•Calibration

•Quality control

•Maintaining and servicing

If deviating from the instructions, the professional user does it at the risk and liability of the laboratory and only after validation by the laboratory. Nihon Kohden has no responsibility over such deviations.

ii	Service Manual MEK-6400/6410/6420

EMC RELATED CAUTION

This equipment and/or system complies with the International Standard EN61326-1 for electromagnetic compatibility for electrical equipment and/or system for measurement, control and laboratory use. However, an electromagnetic environment that exceeds the limits or levels stipulated in the EN61326-1, can cause harmful interference to the equipment and/or system or cause the equipment and/or system to fail to perform its intended function or degrade its intended performance. Therefore, during the operation of the equipment and/or system, if there is any undesired deviation from its intended operational performance, you must avoid, identify and resolve the adverse electromagnetic effect before continuing to use the equipment and/or system.

The following describes some common interference sources and remedial actions:

1.Strong electromagnetic interference from a nearby emitter source such as an authorized radio station or cellular phone:

Install the equipment and/or system at another location if it is interfered with by an emitter source such as an authorized radio station. Keep the emitter source such as cellular phone away from the equipment and/or system.

2.Radio-frequency interference from other equipment through the AC power supply of the equipment and/or system:

Identify the cause of this interference and if possible remove this interference source. If this is not possible, use a different power supply.

3.Effect of direct or indirect electrostatic discharge:

Make sure all users and patients in contact with the equipment and/or system are free from direct or indirect electrostatic energy before using it. A humid room can help lessen this problem.

4.Electromagnetic interference with any radio wave receiver such as radio or television:

If the equipment and/or system interferes with any radio wave receiver, locate the equipment and/or system as far as possible from the radio wave receiver.

If the above suggested remedial actions do not solve the problem, consult your Nihon Kohden Corporation subsidiary or distributor for additional suggestions.

This equipment complies with International Standard EN55011 (1999) Group 1, Class B. Class B EQUIPMENT is equipment suitable for use in domestic establishments and in establishments directly connected to a low voltage power supply network which supplies buildings used for domestic purposes.

The CE mark is a protected conformity mark of the European Community. The products herewith comply with the requirements of the IVD Directive 98/79/EEC.

Service Manual MEK-6400/6410/6420

iii

NOTE about Waste Electrical and Electronic Equipment (WEEE) directive 2002/96/EEC For the member states of the European Union only:

The purpose of WEEE directive 2002/96/EEC is, as a first priority, the prevention of waste electrical and electronic equipment (WEEE), and in addition, the reuse, recycling and other forms of recovery of such wastes so as to reduce the disposal of waste.

Contact your Nihon Kohden representative for disposal at the end of its working life.

iv	Service Manual MEK-6400/6410/6420

Conventions Used in this Manual and Instrument

Warnings, Cautions and Notes

Warnings, cautions and notes are used in this manual to alert or signal the reader to specific information.

WARNING

A warning alerts the user to the possible injury or death associated with the use or misuse of the instrument.

CAUTION

A caution alerts the user to possible injury or problems with the instrument associated with its use or misuse such as instrument malfunction, instrument failure, damage to the instrument, or damage to other property.

NOTE

A note provides specific information, in the form of recommendations, prerequirements, alternative methods or supplemental information.

Service Manual MEK-6400/6410/6420

v

This page is intentionally left blank.

vi	Service Manual MEK-6400/6410/6420

Section 1 General 1

Introduction ........................................................................................................................................................	1.2
Service Policy ....................................................................................................................................................	1.3
Specifications ....................................................................................................................................................	1.4
Measured Parameters, Ranges and Reproducibility to Specimen from Venous Blood.....................	1.4
Standardization Analysis Method ...................................................................................................	1.4
Detection Method ...........................................................................................................................	1.5
Dilution Ratio .................................................................................................................................	1.5
Counting Time ................................................................................................................................	1.5
Display ..........................................................................................................................................	1.5
Data Storage ..................................................................................................................................	1.5
Environmental Conditions ..............................................................................................................	1.5
Power Requirements ......................................................................................................................	1.5
Dimensions and Weight .................................................................................................................	1.6
Electromagnetic Compatibility ........................................................................................................	1.6
Safety ............................................................................................................................................	1.6
Panel Description ...............................................................................................................................................	1.7
Front Panel ...............................................................................................................................................	1.7
Right Side Panel ......................................................................................................................................	1.8
Rear Panel ...............................................................................................................................................	1.9
Composition ......................................................................................................................................................	1.10
MEK-6400 ...............................................................................................................................................	1.10
MEK-6410 ...............................................................................................................................................	1.11
MEK-6420 ...............................................................................................................................................	1.12
Interference Substances ...................................................................................................................................	1.13

Service Manual MEK-6400/6410/6420

1.1

1. GENERAL

Introduction

CAUTION

To maintain the instrument in normal condition, the user must

perform the periodic maintenance. Refer to “Maintenance” of the

operator’s manual.

This service manual provides useful information to qualified service personnel to understand, troubleshoot, service, maintain and repair the MEK-6400/6410/ 6420 Automated Hematology Analyzer (referred to as “the instrument” in this service manual).

The maintenance must be periodically performed because the instrument has fluid paths and precision parts. Accordingly, the user is responsible for performing the periodic maintenance. The “Maintenance” section in this service manual describes the maintenance that should be performed by qualified service personnel. The “Maintenance” section in the operator’s manual describes the maintenance that can be performed by the user.

NOTE

If the instrument has a problem and there has been no periodic maintenance, the instrument will usually be normal again by cleaning the fluid paths or replacing a consumable with a new one.

The information in the operator’s manual is primarily for the user. However, it is important for service personnel to thoroughly read the operator’s manual and service manual before starting to troubleshoot, service, maintain or repair this instrument. This is because service personnel needs to understand the operation of the instrument in order to effectively use the information in the service manual.

For simplicity, the suffix C/J/K will be omitted in this manual. There is no difference in operation and servicing among models with different suffixes unless otherwise specified.

1.2	Service Manual MEK-6400/6410/6420

1. GENERAL

Service Policy

1

CAUTION

•Be careful not to directly touch any place where blood is or may spread to.

•Wear rubber gloves to protect yourself from infection before doing maintenance.

Nihon Kohden Corporation’s basic policy for technical service is to replace faulty units, printed circuit boards or parts. We do not support componentlevel repair of boards and units outside the factory.

NOTE

•When ordering parts or accessories from your nearest Nihon Kohden Corporation’s representative, please quote the NK code number and part name which is listed in this service manual, and the name or model of the unit in which the required part is located. This will help us to promptly attend to your needs.

•Always use parts and accessories recommended or supplied by Nihon Kohden Corporation to assure maximum performance from your instrument.

Service Manual MEK-6400/6410/6420

1.3

1. GENERAL

Specifications

Measured Parameters, Ranges and Reproducibility to Specimen from Venous Blood

Specifications except WBC population were determined using hematology control blood (MEK-3DN), counted 10 times

consecutively.

		Reproducibility to Specimen from
Measured Parameters	Measuring Range	Venous Blood
		(CV: Coefficient of Variation)
WBC: White blood cell count	MEK-6400/6410: 0 to 59 × 103/µL*	within 2.0%CV (4.0 to 9.0 × 10	3	/µL)
	MEK-6420: 0 to 99 × 103/µL
LY%: Lymphocyte percent	0 to 99.9%	within 5.0%CV (WBC: 4.0 to 9.0 × 103/µL,
		LY%: 20 to 45%)
MO%: Monocyte percent	0 to 99.9%	within 12.0%CV (WBC: 4.0 to 9.0 ×
		103/µL, MO%: 2 to 10%)
GR%: Granulocyte percent	0 to 99.9%	within 5.0%CV (WBC: 4.0 to 9.0 × 103/µL,
		GR%: 40 to 70%)
LY: Lymphocyte count	MEK-6400/6410: 0 to 59 × 103/µL*
	MEK-6420: 0 to 99 × 103/µL
MO: Monocyte count	MEK-6400/6410: 0 to 59 × 103/µL*
	MEK-6420: 0 to 99 × 103/µL
GR: Granulocyte count	MEK-6400/6410: 0 to 59 × 103/µL*
	MEK-6420: 0 to 99 × 103/µL
RBC: Red blood cell count	0 to 14.9 × 106/µL	within 1.5%CV (5.0 × 106/µL)
HGB: Hemoglobin concentration	0 to 29.9 g/dL	within 1.5%CV (16 g/dL)
HCT: Hematocrit	0 to 99.9%
MCV: Mean cell volume	20 to 199 fL	within 1.0%CV (70 to 120 fL)
MCH: Mean cell hemoglobin	10 to 50 pg
MCHC: Mean cell hemoglobin	10 to 50 g/dL
concentration
RDW: Red blood cell distribution	0 to 50%
width
PLT: Platelet count	0 to 1490 × 103/µL	within 4.0%CV (3.0 × 103/µL)
PCT: Platelet crit	0 to 2.9%
MPV: Mean platelet volume	0 to 20.0 fL
PDW: Platelet distribution width	0 to 50%

* In panic value recount: 0 to 599 × 103/µL (MEK-6400, MEK-6410 only)

Standardization Analysis Method

WBC: ICSH1988	ICSH: The assignment of values to fresh blood used for calibrating automated blood
	cell counters. Clin Lab Haematol, 10:203-212, 1988
RBC: ICSH1988	ICSH: The assignment of values to fresh blood used for calibrating automated blood
	cell counters. Clin Lab Haematol, 10:203-212, 1988
HGB: NCCLS H15-A2	H15-A2: Reference and Selected Procedures for the Quantitative Determination of
	Hemoglobin in Blood Second Edition; Approved Standard (1994)
HCT: NCCLS H7-A2	H7-A2: Procedure for Determining Packed Cell Volume by the Microhematocrit
	Method Second Edition; Approved Standard (1993)

PLT: Brecher & Cronkite Method: Morphology and enumeration of human blood platelets, J Appl Physiol 3 365 (Dec) 1950; Brecher G, Cronkite EP

1.4	Service Manual MEK-6400/6410/6420

	1. GENERAL
Detection Method	1
Blood cell count:	Electrical resistance detection
Hemoglobin:	Surfactant method (colorimetric method)
Hematocrit:	Histogram calculation
WBC population:	Histogram calculation
Platelet crit:	Histogram calculation
RBC distribution width:	Histogram calculation

Platelet distribution width: Histogram calculation

Dilution Ratio

•Venous blood

Sample volume: 30 µL in normal dilution mode, about 50 µL in low dilution mode, 10 µL in high dilution mode,

		5 µL in higher dilution mode (low and higher dilution modes are not available on the MEK-6420
		analyzer)
WBC/HGB:		200:1 (in normal dilution mode)
RBC/PLT:		40,000:1 (in normal dilution mode)
• Pre-dilution blood
Sample volume:		10 µL	20 µL
WBC/HGB:		1200:1	600:1
RBC/PLT:		240,000:1	120,000:1
Counting Time
Closed mode:	about 90 s/sample (from measurement start to data display, MEK-6400 only)
Open mode:	about 60 s/sample (from measurement start to data display)
Display
Display:		5.7 inch, color LCD with backlight and touch screen keys
Resolution:		240 × 320 dots
Screen size:		approx. 86 × 115 mm
Display contents:		Numerical data, histograms, measuring conditions, alarm message and other messages, touch screen
		keys

Data Storage

Numerical data for all counted parameters for up to 400 samples and histograms for up to 50 samples

Environmental Conditions
Storage temperature:	–20 to 60°C
Operating temperature:	15 to 30°C
Storage humidity:	10 to 95%
Operating humidity:	30 to 85% (Non-condensing)
Storage atmospheric pressure:	70 to 106 kPa
Operating atmospheric pressure: 70 to 106 kPa
Operating altitude:	less than 3000 m
Power Requirements
Power requirements: MEK-6400J, 6410J, 6420J:		110 to 127 V ±10% AC, 50/60 Hz

MEK-6400C/K, 6410C/K, 6420C/K: 220 to 240 V ±10% AC, 50/60 Hz

Power consumption:less than120 VA

Service Manual MEK-6400/6410/6420

1.5

1. GENERAL

Dimensions and Weight

Dimensions: 230 W × 450 D × 383 H (mm)

Net weight: approx. 18 kg

Electromagnetic Compatibility

IEC 61326-1 Edition 1.0: 2002 (Annex A)

EN 61326: 1997/ Amendment 3: 2003

CISPR11 Edition 4.1: 2004, Group 1, Class B

EN 55011: 1998 Amendment 1: 1999, Group 1, Class B

The power supply short interruption test is performed through the transformer which has at least three times the power capacity as the instrument.

Safety

Safety standards: IEC 61010-1 Edition 2.0: 2001

EN 61010-1: 2001

IEC 61010-2-101: 2002

EN 61010-2-101: 2002

IEC 61010-2-081: 2002

EN 61010-2-081: 2002

According to the type of protection against electrical shock:	CLASS I EQUIPMENT
According to the degree of protection against harmful ingress of water:	IPX0 (Ordinary EQUIPMENT)

According to the degree of safety of application in the presence of a FLAMMABLE ANAESTHETIC MIXTURE WITH

AIR, OR WITH OXYGEN OR NITROUS OXIDE:	EQUIPMENT not suitable for use in the presence of FLAMMABLE
	ANAESTHETIC MIXTURE WITH AIR, OR WITH OXYGEN OR
	NITROUSOXIDE
According to the mode of operation:	CONTINUOUS OPERATION
EQUIPMENT types (classification):	Indoor stationary EQUIPMENT
Pollution Degree:	2 EQUIPMENT

Requirements for marking of IN VITRO DIAGNOSTIC instruments: EN1658: 1996

1.6	Service Manual MEK-6400/6410/6420

1. GENERAL

Panel Description	1
Front Panel

	8		8
7	9	7	9
1	10	1	10
2	11	2	11
3		3
4		4
5		5
6	12	6
15	13	15	13
16	14	16	14

		MEK-6400		MEK-6410/6420
	No.	Name	Description
	1	Main power lamp	Lights when the [Main power] switch on the rear panel is tuned on.
	2	Power lamp	Lights when the [Main power] switch on the rear panel and [Power] key on the front panel
			are turned on.
			Turns the analyzer power on or off when the [Main power] switch on the rear panel is turned
	3	Power key	on. When the power is turned on, priming and self-check are automatically performed, and
			the READY screen appears.
	4	Auto print key	Switches the printing mode between automatic and manual for the printer.
	5	Feed key	Feeds paper of the printer while held down.
	6	Print key	Prints displayed data on the printer.
	7	Auto print mode	Lights when automatic printing mode is selected.
		lamp
	8	LCD display	Displays various messages, measured data and touch screen keys.
	9	Reset key	Stops operation when pressed during operation. Returns to the READY screen when pressed
			while changing settings. Use this key only when an error occurs.
			Cleans the fluid path, aperture and manometer with detergent. Automatically primes after
	10	Clean key	cleaning the fluid path. Press this key when clogging occurs, the manometer becomes dirty
			or bubbles occur in the manometer.
		Eject key	For closed mode only. Opens the tube holder to set the sample tube. (MEK-6400)
		Count key	Aspirates the sample and starts counting when <[Eject] key operation> is set to “Count” on
	11		the OPERATION screen of the SETTINGS screen. (MEK-6410, MEK-6420)
		Dispense key	Dispenses the diluent in pre-dilution blood mode when <[Eject] key operation > is set to
			“Dispense” on the OPERATION screen of the SETTINGS screen. (MEK-6410, MEK-6420)
	12	Tube holder	For closed mode only. Holds a sealed vacuum blood collecting tube. Press the [Eject] key to
			open. After measurement, the holder automatically opens. (MEK-6400)
	13	Sampling nozzle	Aspirates the sample. Dispenses the diluent when in the pre-dilution blood mode. (On MEK-
			6400, for open mode only)
	14	Count switch	Aspirates the sample and starts counting.
	15	Printer unit	Thermal array printer. Prints out measured data and sample ID no. (optional)
		(WA-640VK)
	16	Printer door	For the recording paper of the WA-640VK printer unit. To open, pull the upper left corner
			(optional).

Service Manual MEK-6400/6410/6420

1.7

1. GENERAL

Right Side Panel

1																					2	3	4

	No.	Name	Description
	1	ISO3	Inlet for the ISOTONAC•3 diluent.
		Diluent inlet
	2	CLN	Inlet for the CLEANAC detergent.
		Detergent inlet
	3	HEMO3N	Inlet for the Hemolynac•3N hemolysing reagent.
		Hemolysing reagent inlet
	4	WASTE	Outlet for waste such as used lyse, detergent and aspirated
		Waste outlet	samples.

1.8	Service Manual MEK-6400/6410/6420

1. GENERAL

Rear Panel

1

	5
	6
	7
	8
1	9
2	10
3
4	11

	No.	Name	Description
	1	Main power switch	Supplies the power to the analyzer when it is turned on. Under
			normal conditions keep this switch turned on.
	2	Fuse holder	Contains the time lag fuse. To replace the fuse, contact your
			Nihon Kohden representative.
	3	AC SOURCE	Connects the AC power cord to supply AC power to the analyzer.
		AC source socket
	4	Equipotential ground	Connects the ground lead to the equipotential ground terminal on
		terminal	the wall for earth grounding.
			Connects to the optional hand-held bar code reader.
	5	ZK-820V	Supplies the power to the bar code reader when connected.
		Bar code reader socket	Power supply voltage: 5 V DC (pin 9: 5 V, pin 5: GND)
			Rated current: 200 mA
	6	Serial port 1	Connects to the optional WA-460V/461V card printer or PC.
	7	Serial port 2	Connects to the optional WA-460V/461V card printer or PC.
	8	Printer socket	Connects to an external printer (WA-710V/712V or other).
	9	USB socket	Connects to a PC. The optional Data Management Software
			needs to be installed on the PC to receive data from the analyzer.
	10	Memory card socket	Insert a memory card when you upgrade the software.
	11	Memory card eject button	Press this button when you eject a memory card.

Service Manual MEK-6400/6410/6420

1.9

1. GENERAL

Composition

MEK-6400

Standard

MEK-6400J MEK-6400K MEK-6400C

		MC-640V				Measuring Unit
					UT-7198	MEASURING Board
					UT-7201	HGB AMP Board
					UT-7202	HGB LED Board
						2-Way Electromagnetic Value (× 10)
					XP-612V
						2-Way Electromagnetic Value (× 2)
					XP-602V
		MD-640V				Combination Syringe Pump Unit
					UT-7200	MIXED PUMP Board
						2-Way Electromagnetic Value (× 4)
					XP-612V
		MP-640V				Pump Unit
		PV-641VK				Front Panel Unit
					UT-7203	KEY Board
		JQ-640V				Inlet/Outlet Unit
					UT-7199	LIQUID SENSOR Board
						2-Way Electromagnetic Value (× 2)
					XP-612V
		JQ-641V				Valve Unit
						2-Way Electromagnetic Value (× 6)
					XP-612V
		MS-640V				Sampler Unit
		MS-641V				Cap Pierce Unit
		UT-7192				AMP CONTROL Board
		UT-7193				POWER Board

Option

WA-640VK				Printer Unit
			UT-7205	PRINTER DRIVER Board

1.10	Service Manual MEK-6400/6410/6420

1. GENERAL

MEK-6410

1

Standard

MEK-6410J MEK-6410K MEK-6410C

		MC-640V				Measuring Unit
					UT-7198	MEASURING Board
					UT-7201	HGB AMP Board
					UT-7202	HGB LED Board
						2-Way Electromagnetic Value (× 10)
					XP-612V
						2-Way Electromagnetic Value (× 2)
					XP-602V
		MD-640V				Combination Syringe Pump Unit
					UT-7200	MIXED PUMP Board
						2-Way Electromagnetic Value (× 4)
					XP-612V
		MP-640V				Pump Unit
		PV-641VK				Front Panel Unit
					UT-7203	KEY Board
		JQ-640V				Inlet/Outlet Unit
					UT-7199	LIQUID SENSOR Board
						2-Way Electromagnetic Value (× 2)
					XP-612V
		JQ-642V				Valve Unit
						2-Way Electromagnetic Value (× 4)
					XP-612V
		MS-640V				Sampler Unit
		MR-640V				Rinse Unit
		UT-71921				AMP CONTROL Board
		UT-7193				POWER Board

Option

WA-640VK	Printer Unit

UT-7205 PRINTER DRIVER Board

Service Manual MEK-6400/6410/6420

1.11

1. GENERAL

MEK-6420

Standard

MEK-6420J MEK-6420K MEK-6420C

	MC-640V				Measuring Unit
				UT-7198	MEASURING Board
				UT-7201	HGB AMP Board
				UT-7202	HGB LED Board
				XP-612V	2-Way Electromagnetic Value (x 10)
				XP-602V	2-Way Electromagnetic Value (x 2)
	MD-640V				Combination Syringe Pump Unit
				UT-7200	MIXED PUMP Board
				XP-612V	2-Way Electromagnetic Value (x 4)
	MP-640V				Pump Unit
	PV-641VK				Front Panel Unit
				UT-7203	KEY Board
	JQ-640V				Inlet/Outlet Unit
				UT-7199	LIQUID SENSOR Board
				XP-612V	2-Way Electromagnetic Value (x 2)
	JQ-642V				Valve Unit
				XP-612V	2-Way Electromagnetic Value (x 4)
	MS-640V				Sampler Unit
	MR-640V				Rinse Unit
	UT-71922				AMP CONTROL Board (MEK-6420J/K)
	UT-71923				AMP CONTROL Board (MEK-6420C)
	UT-7193				POWER Board

Option

WA-640VK				Printer Unit
			UT-7205	PRINTER DRIVER Board

1.12	Service Manual MEK-6400/6410/6420

Interference Substances

1. GENERAL

1

WBC: High WBC

When WBC count is outside the measurable range, measure the sample in high dilution mode. If the WBC count is still outside the range, dilute the sample further.

Nucleated erythrocyte

Nucleated erythrocyte is detected as WBC and causes increase in WBC count.

Unlysed red cells

In some rare occasions, the RBC in the blood sample may not completely lyse and these non-lysed RBC may be detected as WBC and cause increase in WBC count.

Multiple myeloma

The precipitation of proteins in multiple myeloma patients may increase the WBC count.

Leukemia

WBC is fragile in leukemia patients and WBC may be destroyed during measurement. These WBC fragments may also interfere with WBC differential measurement.

Chemotherapy

Cytotoxic and immunosuppressive drugs cause low WBC count.

Cryoglobulins

Cryoglobulin may be increased in patients who are pregnant or have myeloma, cancer, leukemia, macroglobulinemia, lymphoproliferative disorders, metastatic tumors, autoimmune disorders, infections, aneurysm, thromboembolic phenomena, diabetes, etc, which cause increase in WBC, RBC or PLT counts and HGB concentration. In such cases, warm the blood sample to 37°C in a water bath for 30 minutes and measure the sample immediately.

RBC: Leukemia

An increase in WBC in leukemia patient causes increase in RBC.

Agglutinated RBC

Agglutinated RBC may decrease RBC count. This can be checked by abnormal MCH and MCHC values and examination of the stained blood film.

Cold agglutinins

IgM immunoglobulins which are elevated in clod agglutinin disease may decrease RBC and PLT counts and increase MCV.

Hemolysis

When RBC is hemolyzed, RBC is decreased.

HGB: Turbidity of the blood sample

Any physiologic and/or therapeutic factors may increase HGB. In such a case, determine the cause of turbidity and follow the appropriate method below.

1.Increased WBC

An extreme increase in WBC causes excessive light scatter. In these cases, measure manually. Centrifuge the diluted sample and measure the supernatant fluid with a spectrophotometer.

2.Increased lipids

The blood sample may be milky when there is excessive lipids. This may occur with hyperlipidemia, hyperproteinemia and hyperbilirubinemia. Accurate HGB measurement can be achieved by manual methods and a plasma blank.

3.Increased turbidity

When RBC are resistant to lysing, turbidity may increase causing increase in HGB. Observe if MCH and MCHC values are abnormal. HGB result affects MCH and MCHC result.

4.Fetal bloods

The mixing of fetal and maternal blood may increase HGB value.

Service Manual MEK-6400/6410/6420

1.13

1.GENERAL

5.High WBC levels

Turbidity of blood increases and the hemoglobin concentration becomes high if WBC level of the blood sample is high. MCH and MCHC levels also become high.

HCT: Agglutinated RBC

RBC agglutination may cause erroneous HCT and MCV values. Observe if MCH and MCHC values are abnormal. In such a case, measure manually.

MCV: Agglutinated RBC

RBC agglutination may cause erroneous HCT and MCV values. Observe if MCH and MCHC values are abnormal. In such a case, measure manually.

Excessive number of large PLT

Excessive number of large PLT and/or excessively high WBC may affect the MCV value. Check by careful examination of the stained blood film.

MCH: MCH is determined from HGB and RBC values. Therefore, the limitations for HGB and RBC also affect MCH value.

MCHC: MCHC is determined from HGB and HCT values. Therefore, the limitations for HGB and HCT also affect

	MCHC value.
RDW:	RDW is determined from RBC value. Therefore, the limitations for RBC also affect RDW value.
	Agglutinated RBC
	Agglutinated RBC may decrease RBC count and erroneous RDW. This can be checked by abnormal MCH and
	MCHC values and examination of the stained blood film.
	Nutritional deficiency or blood transfusion
	Iron and/or cobalamin and/or folate deficiency may increase RDW.
PLT:	Very small fragments
	Very small RBC, RBC fragments and WBC fragments may be the cause in increased PLT count.
	Agglutinated RBC
	PLT may be trapped in the agglutinated RBC resulting in decrease in PLT. This can be checked by abnormal
	MCH and MCHC values and examination of the stained blood film.
	Very large PLT
	Large PLT may exceed the PLT threshold and might not be counted which results in low PLT count.
	Chemotherapy
	Cytotoxic and immunosuppressive drugs may increase the fragility of cells which may cause low PLT count.
	In such a case, measure manually.
	Hemolysis
	Hemolyzed specimens contain red cell stroma which may increase PLT count.
	Anticoagulated blood
	Blood anticoagulated with acid-citrate-dextrose may have clumped PLT which may cause decrease in PLT
	count.
	Agglutinated PLT
	Clumped PLT may decrease PLT count and/or increase WBC count. For such sample, collect the sample in
	sodium citrate anticoagulant and measure only PLT. The PLT result must be corrected for the sodium citrate
	dilution effect.
MPV:	Very large PLT
	Large PLT may exceed the PLT threshold and not be counted which results in low MPV.
	Very small fragments
	Very small RBC, RBC fragments and WBC fragments may interfere with MPV measurement.
	Agglutinated RBC
	PLT may be trapped in the agglutinated RBC resulting in erroneous MPV. This can be checked by abnormal
	MCH and MCHC values and examination of the stained blood film.

1.14	Service Manual MEK-6400/6410/6420

1. GENERAL

Chemotherapy

1

Cytotoxic and immunosuppressive drugs may affect MPV. In such a case, measure manually.

NOTE

Blood samples collected in EDTA do not maintain stable MPV because platelets swell

depending on the interval after collection and storage temperature.

WBC differential parameters are derived from the WBC count, therefore, the limitations for WBC also affect these parameters.

LY and LY%: Erythroblasts, certain parasites and RBC that are resistant to lysis may interfere with an accurate LY count.

MO and MO%: Large lymphocytes, atypical lymphocytes, blasts and excessive number of basophils may interfere with an accurate MO count.

GR and GR%: Excessive eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may interfere with an accurate GR count and GR%.

EO: Abnormal granules may interfere with an accurate EO count.

Service Manual MEK-6400/6410/6420

1.15

Section 2	Troubleshooting	2
Check Procedure Flowchart ...............................................................................................................................	2.4
Operating Condition and Blood Sample Checks .................................................................................................	2.5
Operating Condition Check ......................................................................................................................	2.5
Blood Sample Handling Check .................................................................................................................	2.5
Pre-dilution Sample Preparation Check ....................................................................................................	2.5
Checking the Hematology Analyzer ...................................................................................................................	2.7
Checking Background Noise ....................................................................................................................	2.7
Measurement .................................................................................................................................	2.7
Parameter Data Check with Diluent ................................................................................................	2.7
Reducing Background Noise ..........................................................................................................	2.8
Background Noise Problems ..........................................................................................................	2.9
Checking the Reproducibility ....................................................................................................................	2.9
Checking Procedure .......................................................................................................................	2.9
CV Values .....................................................................................................................................	2.10
Checking Accuracy ...........................................................................................................................................	2.11
Checking Procedure ................................................................................................................................	2.11
Poor WBC Reproducibility .......................................................................................................................	2.11
Poor HGB Reproducibility ........................................................................................................................	2.12
Poor RBC Reproducibility ........................................................................................................................	2.13
Poor PLT Reproducibility .........................................................................................................................	2.14
Poor HCT or MCV Reproducibility ...........................................................................................................	2.15
Solving Problems from Alarm Messages ..........................................................................................................	2.16
A001: No diluent ...........................................................................................................................	2.16
A005: No detergent .......................................................................................................................	2.16
A007: No lysing reagent ................................................................................................................	2.17
A009: WBC priming error ...............................................................................................................	2.17
A010: RBC PRIMING ERROR ......................................................................................................	2.17
A021: WBC level 1 ........................................................................................................................	2.17
A022: WBC level 2 ........................................................................................................................	2.18
A023: WBC level 3 ........................................................................................................................	2.18
A024: WBC bubble 1 .....................................................................................................................	2.18
A025: WBC bubble 2 .....................................................................................................................	2.18
A026: WBC bubble 3 .....................................................................................................................	2.19
A027: WBC bubble 4 .....................................................................................................................	2.19
A029: WBC clog ............................................................................................................................	2.19
A030: WBC sample error ...............................................................................................................	2.19
A031: WBC noise 2 .......................................................................................................................	2.20
A032: WBC noise 1 .......................................................................................................................	2.20
A036: WBC upper manometer dirty ...............................................................................................	2.20
A037: WBC lower manometer dirty ................................................................................................	2.20
A041: RBC level 1 ........................................................................................................................	2.21
A042: RBC level 2 ........................................................................................................................	2.21
A043: RBC level 3 ........................................................................................................................	2.21
A044: RBC bubble 1 ......................................................................................................................	2.22
A045: RBC bubble 2 ......................................................................................................................	2.22
A046: RBC bubble 3 ......................................................................................................................	2.22
Service Manual MEK-6400/6410/6420	2.1