Metrohm 940 User Manual

940 Professional IC Vario

940 Professional IC Vario ONE/SeS/HPG

Manual – Short Instructions

8.940.8116EN

Metrohm AG
CH-9100 Herisau
Switzerland
Phone [phone]
Fax [phone]
[e-mail]
www.metrohm.com

940 Professional IC Vario

940 Professional IC Vario ONE/SeS/

HPG

2.940.1440

8.940.8116EN

Manual – Short Instructions

11.2013 zst

Teachware
Metrohm AG
CH-9100 Herisau
[e-mail]

This documentation is protected by copyright. All rights reserved.

Although all the information given in this documentation has been
checked with great care, errors cannot be entirely excluded. Should you
notice any mistakes please send us your comments using the address
given above.

Documentation in additional languages can be found on

http://documents.metrohm.com.

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Table of contents

1 About these Short Instructions 1

2 Introduction 2

2.1 Instrument description ......................................................... 2

2.2 Intended use ......................................................................... 4

2.3 Safety instructions ................................................................ 5

2.3.1 General notes on safety ........................................................... 5

2.3.2 Electrical safety ........................................................................ 5

2.3.3 Tubing and capillary connections ............................................. 6

2.3.4 Flammable solvents and chemicals ........................................... 6

2.3.5 Recycling and disposal ............................................................. 6

2.4 Symbols and conventions .................................................... 7

3 Overview of the instrument 8

Table of contents

3.1 Front ...................................................................................... 8

3.2 Rear ...................................................................................... 10

4 Installation 12

4.1 Setting up the instrument .................................................. 12

4.1.1 Packaging .............................................................................. 12

4.1.2 Checks .................................................................................. 12

4.1.3 Location ................................................................................ 12

4.2 Removing the handle ......................................................... 12

4.3 Removing transport locking screws ................................. 13

4.4 Connecting the drainage tubing and leak sensor ............ 15

4.4.1 Installing the drainage tubing ................................................ 15

4.4.2 Connecting the leak sensor .................................................... 16

4.5 Column thermostat ............................................................ 17

4.6 Connecting the eluent bottle ............................................. 17

4.7 Connecting the eluent degasser ........................................ 19

4.8 Installing the high-pressure pump .................................... 19

4.9 Installing an inline filter ..................................................... 19

4.10 Installing the pulsation absorber ...................................... 19

4.11 Injection valve ..................................................................... 19

4.12 Suppressor .......................................................................... 19

4.12.1 Inserting the rotors ................................................................ 20

4.12.2 Connecting the suppressor .................................................... 21

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Table of contents

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4.13 Metrohm CO2 Suppressor (MCS) ....................................... 24

4.13.1 Connecting the MCS ............................................................. 24

4.13.2 Installing adsorption cartridges .............................................. 24

4.14 Installing the detector ........................................................ 26

4.15 Connecting the sample degasser (optional) ..................... 27

4.16 Installing the high-pressure gradient module .................. 28

4.17 Connecting the instrument to a computer ....................... 28

4.18 Connecting the instrument to the power supply ............. 29

4.19 Initial start-up ..................................................................... 30

4.20 Connecting and rinsing the guard column ....................... 32

4.21 Connecting the separation column ................................... 34

4.22 Conditioning ........................................................................ 36

5 Operation 38

6 Warranty (guarantee) 39

7 Accessories 41

Index 43

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Table of figures

Figure 1 Front ................................................................................................. 8

Figure 2 Rear ................................................................................................ 10

Figure 3 Removing the transport locking screws ............................................ 14

Figure 4 Suppressor – connection capillaries ................................................. 22

Figure 5 Connecting the MCS ....................................................................... 24

Table of figures

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1 About these Short Instructions

This short instruction manual contains important chapters from the com­prehensive manual. In addition to an introduction, safety instructions and
an overview of the instrument, you will also find information about instal­ling and operating the 940 Professional IC Vario ONE/SeS/HPG as well as
information regarding the warranty. You can download the detailed man­ual as a PDF file from the Internet.

Downloading the manual

You can find the detailed manual on the Internet under http://docu-

ments.metrohm.com.

1. Click on Instruments ▶ Ion Chromatography.

2. Select the line of instruments, e.g. 940 Professional IC Vario.

3. Select the instrument.

All available manuals for the instrument will be displayed.

1 About these Short Instructions

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2.1 Instrument description

2 Introduction

2.1 Instrument description

The 940 Professional IC Vario is a professional ion chromatograph. It is
distinguished by:

￭ Its intelligence: All of the functions are monitored, optimized and

documented in an FDA-compatible manner. Intelligent components,
such as iColumns, save important data onto a chip.

￭ Its compact design: It has a small footprint.
￭ Its modularity: It provides flexibility for use in various applications. It

can hold up to three modules for different functions in its three draw­ers. Individual modules can be swapped or added as needed.

￭ Its transparency: All components are easily accessible and located for

simple visibility and can be monitored during operation through a large
window.

￭ Its safety: The design separates the wet end and the electronics,

thereby preventing liquids from coming into contact with the electron­ics to a large extent. A leak sensor is integrated into the wet end.

￭ Its environmental compatibility.
￭ Its low noise emissions.
￭ The intelligent MagIC Net software

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The 940 Professional IC Vario is operated solely using the MagIC Net soft­ware. A USB cable is used to connect the instrument to a computer with
MagIC Net installed. The intelligent software detects the instrument auto­matically and checks its functionality. The software controls and monitors
the instrument, evaluates the measured data and manages it in a data­base.

The 940 Professional IC Vario ONE/SeS/HPG consists of the following
modules:

Housing

The sturdy housing contains the instrument's electronic components,
including their interfaces and three connections for separation columns
(two of which are built into the installed column thermostat). In addition,
the housing provides space for two detectors and up to three plug-ins
with different functions. Capillaries and cables can be fed into and out of
the instrument through several openings.

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2 Introduction

Leak sensor

The leak sensor detects leaking liquid that collects in the instrument's base
tray. Liquid that leaks in the instrument is routed to the base tray using
drainage tubing and detected there.

Column thermostat

The column thermostat regulates the temperature for the separation col­umns and the eluent, thereby providing stable measuring conditions. The
interior of the column thermostat can be heated and cooled. There are
two column holders with chip readers in the column thermostat.

Eluent degasser

The eluent degasser removes gas bubbles and dissolved gases from the
eluent.

High-pressure pump

The intelligent and low-pulsation high-pressure pump pumps the eluent
through the system. It is equipped with a chip where its technical specifi­cations and "life history" (operating hours, service data, etc.) are saved.

Inline filter

Inline filters protect the separation column reliably from potential contami­nation from the eluent. The small filter pads with 2 µm pore size can be
replaced quickly and easily. They remove particles from the solutions, such
as bacteria and algae.

Pulsation absorber

The pulsation absorber protects the separation column from damage
caused by pressure fluctuations when switching the injection valve, and
reduces interfering pulsations during highly sensitive measurements.

Injection valve

The injection valve connects the eluent path to the sample path. By a
quick and precise switching of the valve a quantity of sample solution
defined by the size of the sample loop is injected and flushed to the sepa­ration column with the eluent.

Suppressor

The suppressor consists of a suppressor drive, a rotor and, where applica­ble, an adapter. The suppressor drive gives you the flexibility to use differ­ent rotors according to the principle "one drive – many rotors". With
appropriate adapters, the rotor for the sample preparation module (SPM
Rotor) or suppressor rotors with different capacities and construction can
be easily exchanged. The rotors are not included in the instrument's scope
of delivery. A rotor suitable for the application must be ordered separately.

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2.2 Intended use

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Metrohm CO2 Suppressor (MCS)

The Metrohm CO2 Suppressor (MCS) removes the CO2 from the eluent
flow. This lowers the background conductivity, improves detection sensi­tivity and minimizes the injection peak and carbonate peak.

Detector

Metrohm offers a series of different detectors for various analysis tasks. A
suitable detector type must be ordered as a separate accessory.

Sample degasser

The sample degasser removes gas bubbles and dissolved gases from the
sample.

High-pressure gradient module (HPG)

The second high-pressure pump in the instrument allows a gradient to be
created from two eluents. The software is used to control the amount of
eluent.

Separation column

The intelligent separation column separates different components accord­ing to their interactions with the column. Metrohm separation columns
are equipped with a chip where their technical specifications and history
(start-up, operating hours, etc) are stored.

2.2 Intended use

The 940 Professional IC Vario ONE/SeS/HPG is used for the determination
of anions or polar substances using sequential suppression using ion chro­matography when the complex separation problem requires the use of
gradients.

Sequential suppression consists of:

￭ Chemical suppression with a Metrohm Suppressor Module (MSM) and

subsequent

￭ CO2 suppression with the Metrohm CO2 Suppressor (MCS).

Background conductivity is reduced to a minimum with sequential sup­pression.

The second high-pressure pump in the lower plug-in allows for the con­trolled mixing of two eluents.

The instrument can also be used as needed for the determination of cati­ons or anions without chemical suppression.

The present instrument is suitable for processing chemicals and flammable
samples. Usage of the 940 Professional IC Vario therefore requires the

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user to have basic knowledge and experience in handling toxic and caustic
substances. Knowledge with respect to the application of the fire preven­tion measures prescribed for laboratories is also mandatory.

2.3 Safety instructions

2.3.1 General notes on safety

WARNING

This instrument may only be operated in accordance with the specifica­tions in this documentation.

This instrument has left the factory in a flawless state in terms of technical
safety. To maintain this state and ensure non-hazardous operation of the
instrument, the following instructions must be observed carefully.

2.3.2 Electrical safety

The electrical safety when working with the instrument is ensured as part
of the international standard IEC 61010.

2 Introduction

WARNING

Only personnel qualified by Metrohm are authorized to carry out service
work on electronic components.

WARNING

Never open the housing of the instrument. The instrument could be
damaged by this. There is also a risk of serious injury if live components
are touched.

There are no parts inside the housing which can be serviced or replaced
by the user.

Mains voltage

WARNING

An incorrect mains voltage can damage the instrument.

Only operate this instrument with a mains voltage specified for it (see
rear panel of the instrument).

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2.3 Safety instructions

Protection against electrostatic charges

WARNING

Electronic components are sensitive to electrostatic charges and can be
destroyed by discharges.

Do not fail to pull the mains cable out of the mains connection socket
before you set up or disconnect electrical plug connections at the rear
of the instrument.

2.3.3 Tubing and capillary connections

CAUTION

Leaks in tubing and capillary connections are a safety risk. Tighten all
connections well by hand. Avoid applying excessive force to tubing
connections. Damaged tubing ends lead to leakage. Appropriate tools
can be used to loosen connections.

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Check the connections regularly for leakage. If the instrument is used
mainly in unattended operation, then weekly inspections are manda­tory.

2.3.4 Flammable solvents and chemicals

WARNING

All relevant safety measures are to be observed when working with
flammable solvents and chemicals.

￭ Set up the instrument in a well-ventilated location (e.g. fume cup-

board).

￭ Keep all sources of flame far from the workplace.
￭ Clean up spilled liquids and solids immediately.
￭ Follow the safety instructions of the chemical manufacturer.

2.3.5 Recycling and disposal

This product is covered by European Directive 2002/96/EC, WEEE – Waste
from Electrical and Electronic Equipment.

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The correct disposal of your old equipment will help to prevent negative
effects on the environment and public health.

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More details about the disposal of your old equipment can be obtained
from your local authorities, from waste disposal companies or from your
local dealer.

2.4 Symbols and conventions

The following symbols and formatting may appear in this documentation:

Cross-reference to figure legend

The first number refers to the figure number, the sec­ond to the instrument part in the figure.

Instruction step

Carry out these steps in the sequence shown.

Method Dialog text, parameter in the software

File ▶ New Menu or menu item

[Next] Button or key

2 Introduction

WARNING

This symbol draws attention to a possible life-threat­ening hazard or risk of injury.

WARNING

This symbol draws attention to a possible hazard due
to electrical current.

WARNING

This symbol draws attention to a possible hazard due
to heat or hot instrument parts.

WARNING

This symbol draws attention to a possible biological
hazard.

CAUTION

This symbol draws attention to possible damage to
instruments or instrument parts.

NOTE

940 Professional IC Vario ONE/SeS/HPG (2.940.1440)

This symbol highlights additional information and
tips.

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3.1 Front

3 Overview of the instrument

3.1 Front

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1

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Figure 1 Front

Bottle holder

Offers space for the eluent bottle and addi­tional accessories.

Column holder

For a third separation column outside the
column thermostat.

Detector chamber

2

Offers space for two embedded detectors
and additional accessories.

Metrohm CO2 Suppressor (MCS)

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940 Professional IC Vario ONE/SeS/HPG (2.940.1440)