Metrohm 940 User Manual

940 Professional IC Vario
940 Professional IC Vario ONE/SeS/HPG
Manual – Short Instructions
8.940.8116EN
Metrohm AG CH-9100 Herisau Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 info@metrohm.com www.metrohm.com
940 Professional IC Vario
940 Professional IC Vario ONE/SeS/
HPG
2.940.1440
8.940.8116EN
Manual – Short Instructions
11.2013 zst
Teachware Metrohm AG CH-9100 Herisau teachware@metrohm.com
This documentation is protected by copyright. All rights reserved.
Although all the information given in this documentation has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please send us your comments using the address given above.
Documentation in additional languages can be found on
http://documents.metrohm.com.
■■■■■■■■■■■■■■■■■■■■■■

Table of contents

1 About these Short Instructions 1
2 Introduction 2
2.1 Instrument description ......................................................... 2
2.2 Intended use ......................................................................... 4
2.3 Safety instructions ................................................................ 5
2.3.1 General notes on safety ........................................................... 5
2.3.2 Electrical safety ........................................................................ 5
2.3.3 Tubing and capillary connections ............................................. 6
2.3.4 Flammable solvents and chemicals ........................................... 6
2.3.5 Recycling and disposal ............................................................. 6
2.4 Symbols and conventions .................................................... 7
3 Overview of the instrument 8
Table of contents
3.1 Front ...................................................................................... 8
3.2 Rear ...................................................................................... 10
4 Installation 12
4.1 Setting up the instrument .................................................. 12
4.1.1 Packaging .............................................................................. 12
4.1.2 Checks .................................................................................. 12
4.1.3 Location ................................................................................ 12
4.2 Removing the handle ......................................................... 12
4.3 Removing transport locking screws ................................. 13
4.4 Connecting the drainage tubing and leak sensor ............ 15
4.4.1 Installing the drainage tubing ................................................ 15
4.4.2 Connecting the leak sensor .................................................... 16
4.5 Column thermostat ............................................................ 17
4.6 Connecting the eluent bottle ............................................. 17
4.7 Connecting the eluent degasser ........................................ 19
4.8 Installing the high-pressure pump .................................... 19
4.9 Installing an inline filter ..................................................... 19
4.10 Installing the pulsation absorber ...................................... 19
4.11 Injection valve ..................................................................... 19
4.12 Suppressor .......................................................................... 19
4.12.1 Inserting the rotors ................................................................ 20
4.12.2 Connecting the suppressor .................................................... 21
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
III
Table of contents
■■■■■■■■■■■■■■■■■■■■■■
4.13 Metrohm CO2 Suppressor (MCS) ....................................... 24
4.13.1 Connecting the MCS ............................................................. 24
4.13.2 Installing adsorption cartridges .............................................. 24
4.14 Installing the detector ........................................................ 26
4.15 Connecting the sample degasser (optional) ..................... 27
4.16 Installing the high-pressure gradient module .................. 28
4.17 Connecting the instrument to a computer ....................... 28
4.18 Connecting the instrument to the power supply ............. 29
4.19 Initial start-up ..................................................................... 30
4.20 Connecting and rinsing the guard column ....................... 32
4.21 Connecting the separation column ................................... 34
4.22 Conditioning ........................................................................ 36
5 Operation 38
6 Warranty (guarantee) 39
7 Accessories 41
Index 43
■■■■■■■■
IV
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■

Table of figures

Figure 1 Front ................................................................................................. 8
Figure 2 Rear ................................................................................................ 10
Figure 3 Removing the transport locking screws ............................................ 14
Figure 4 Suppressor – connection capillaries ................................................. 22
Figure 5 Connecting the MCS ....................................................................... 24
Table of figures
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
V
■■■■■■■■■■■■■■■■■■■■■■
1 About these Short Instructions
This short instruction manual contains important chapters from the com­prehensive manual. In addition to an introduction, safety instructions and an overview of the instrument, you will also find information about instal­ling and operating the 940 Professional IC Vario ONE/SeS/HPG as well as information regarding the warranty. You can download the detailed man­ual as a PDF file from the Internet.
Downloading the manual
You can find the detailed manual on the Internet under http://docu-
ments.metrohm.com.
1. Click on Instruments Ion Chromatography.
2. Select the line of instruments, e.g. 940 Professional IC Vario.
3. Select the instrument.
All available manuals for the instrument will be displayed.

1 About these Short Instructions

940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
1

2.1 Instrument description

2 Introduction

2.1 Instrument description
The 940 Professional IC Vario is a professional ion chromatograph. It is distinguished by:
Its intelligence: All of the functions are monitored, optimized and
documented in an FDA-compatible manner. Intelligent components, such as iColumns, save important data onto a chip.
Its compact design: It has a small footprint. Its modularity: It provides flexibility for use in various applications. It
can hold up to three modules for different functions in its three draw­ers. Individual modules can be swapped or added as needed.
Its transparency: All components are easily accessible and located for
simple visibility and can be monitored during operation through a large window.
Its safety: The design separates the wet end and the electronics,
thereby preventing liquids from coming into contact with the electron­ics to a large extent. A leak sensor is integrated into the wet end.
Its environmental compatibility. Its low noise emissions. The intelligent MagIC Net software
■■■■■■■■■■■■■■■■■■■■■■
The 940 Professional IC Vario is operated solely using the MagIC Net soft­ware. A USB cable is used to connect the instrument to a computer with MagIC Net installed. The intelligent software detects the instrument auto­matically and checks its functionality. The software controls and monitors the instrument, evaluates the measured data and manages it in a data­base.
The 940 Professional IC Vario ONE/SeS/HPG consists of the following modules:
Housing
The sturdy housing contains the instrument's electronic components, including their interfaces and three connections for separation columns (two of which are built into the installed column thermostat). In addition, the housing provides space for two detectors and up to three plug-ins with different functions. Capillaries and cables can be fed into and out of the instrument through several openings.
■■■■■■■■
2
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
2 Introduction
Leak sensor
The leak sensor detects leaking liquid that collects in the instrument's base tray. Liquid that leaks in the instrument is routed to the base tray using drainage tubing and detected there.
Column thermostat
The column thermostat regulates the temperature for the separation col­umns and the eluent, thereby providing stable measuring conditions. The interior of the column thermostat can be heated and cooled. There are two column holders with chip readers in the column thermostat.
Eluent degasser
The eluent degasser removes gas bubbles and dissolved gases from the eluent.
High-pressure pump
The intelligent and low-pulsation high-pressure pump pumps the eluent through the system. It is equipped with a chip where its technical specifi­cations and "life history" (operating hours, service data, etc.) are saved.
Inline filter
Inline filters protect the separation column reliably from potential contami­nation from the eluent. The small filter pads with 2 µm pore size can be replaced quickly and easily. They remove particles from the solutions, such as bacteria and algae.
Pulsation absorber
The pulsation absorber protects the separation column from damage caused by pressure fluctuations when switching the injection valve, and reduces interfering pulsations during highly sensitive measurements.
Injection valve
The injection valve connects the eluent path to the sample path. By a quick and precise switching of the valve a quantity of sample solution defined by the size of the sample loop is injected and flushed to the sepa­ration column with the eluent.
Suppressor
The suppressor consists of a suppressor drive, a rotor and, where applica­ble, an adapter. The suppressor drive gives you the flexibility to use differ­ent rotors according to the principle "one drive – many rotors". With appropriate adapters, the rotor for the sample preparation module (SPM Rotor) or suppressor rotors with different capacities and construction can be easily exchanged. The rotors are not included in the instrument's scope of delivery. A rotor suitable for the application must be ordered separately.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
3

2.2 Intended use

■■■■■■■■■■■■■■■■■■■■■■
Metrohm CO2 Suppressor (MCS)
The Metrohm CO2 Suppressor (MCS) removes the CO2 from the eluent flow. This lowers the background conductivity, improves detection sensi­tivity and minimizes the injection peak and carbonate peak.
Detector
Metrohm offers a series of different detectors for various analysis tasks. A suitable detector type must be ordered as a separate accessory.
Sample degasser
The sample degasser removes gas bubbles and dissolved gases from the sample.
High-pressure gradient module (HPG)
The second high-pressure pump in the instrument allows a gradient to be created from two eluents. The software is used to control the amount of eluent.
Separation column
The intelligent separation column separates different components accord­ing to their interactions with the column. Metrohm separation columns are equipped with a chip where their technical specifications and history (start-up, operating hours, etc) are stored.
2.2 Intended use
The 940 Professional IC Vario ONE/SeS/HPG is used for the determination of anions or polar substances using sequential suppression using ion chro­matography when the complex separation problem requires the use of gradients.
Sequential suppression consists of:
Chemical suppression with a Metrohm Suppressor Module (MSM) and
subsequent
CO2 suppression with the Metrohm CO2 Suppressor (MCS).
Background conductivity is reduced to a minimum with sequential sup­pression.
The second high-pressure pump in the lower plug-in allows for the con­trolled mixing of two eluents.
The instrument can also be used as needed for the determination of cati­ons or anions without chemical suppression.
The present instrument is suitable for processing chemicals and flammable samples. Usage of the 940 Professional IC Vario therefore requires the
■■■■■■■■
4
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
user to have basic knowledge and experience in handling toxic and caustic substances. Knowledge with respect to the application of the fire preven­tion measures prescribed for laboratories is also mandatory.

2.3 Safety instructions

2.3.1 General notes on safety

WARNING
This instrument may only be operated in accordance with the specifica­tions in this documentation.
This instrument has left the factory in a flawless state in terms of technical safety. To maintain this state and ensure non-hazardous operation of the instrument, the following instructions must be observed carefully.

2.3.2 Electrical safety

The electrical safety when working with the instrument is ensured as part of the international standard IEC 61010.
2 Introduction
WARNING
Only personnel qualified by Metrohm are authorized to carry out service work on electronic components.
WARNING
Never open the housing of the instrument. The instrument could be damaged by this. There is also a risk of serious injury if live components are touched.
There are no parts inside the housing which can be serviced or replaced by the user.
Mains voltage
WARNING
An incorrect mains voltage can damage the instrument.
Only operate this instrument with a mains voltage specified for it (see rear panel of the instrument).
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
5
2.3 Safety instructions
Protection against electrostatic charges
WARNING
Electronic components are sensitive to electrostatic charges and can be destroyed by discharges.
Do not fail to pull the mains cable out of the mains connection socket before you set up or disconnect electrical plug connections at the rear of the instrument.

2.3.3 Tubing and capillary connections

CAUTION
Leaks in tubing and capillary connections are a safety risk. Tighten all connections well by hand. Avoid applying excessive force to tubing connections. Damaged tubing ends lead to leakage. Appropriate tools can be used to loosen connections.
■■■■■■■■■■■■■■■■■■■■■■
Check the connections regularly for leakage. If the instrument is used mainly in unattended operation, then weekly inspections are manda­tory.

2.3.4 Flammable solvents and chemicals

WARNING
All relevant safety measures are to be observed when working with flammable solvents and chemicals.
Set up the instrument in a well-ventilated location (e.g. fume cup-
board).
Keep all sources of flame far from the workplace. Clean up spilled liquids and solids immediately. Follow the safety instructions of the chemical manufacturer.

2.3.5 Recycling and disposal

This product is covered by European Directive 2002/96/EC, WEEE – Waste from Electrical and Electronic Equipment.
■■■■■■■■
6
The correct disposal of your old equipment will help to prevent negative effects on the environment and public health.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
More details about the disposal of your old equipment can be obtained from your local authorities, from waste disposal companies or from your local dealer.

2.4 Symbols and conventions

The following symbols and formatting may appear in this documentation:
Cross-reference to figure legend
The first number refers to the figure number, the sec­ond to the instrument part in the figure.
Instruction step
Carry out these steps in the sequence shown.
Method Dialog text, parameter in the software
File New Menu or menu item
[Next] Button or key
2 Introduction
WARNING
This symbol draws attention to a possible life-threat­ening hazard or risk of injury.
WARNING
This symbol draws attention to a possible hazard due to electrical current.
WARNING
This symbol draws attention to a possible hazard due to heat or hot instrument parts.
WARNING
This symbol draws attention to a possible biological hazard.
CAUTION
This symbol draws attention to possible damage to instruments or instrument parts.
NOTE
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
This symbol highlights additional information and tips.
■■■■■■■■
7

3.1 Front

3 Overview of the instrument

3.1 Front
■■■■■■■■■■■■■■■■■■■■■■
1
3
■■■■■■■■
8
Figure 1 Front
Bottle holder
Offers space for the eluent bottle and addi­tional accessories.
Column holder
For a third separation column outside the column thermostat.
Detector chamber
2
Offers space for two embedded detectors and additional accessories.
Metrohm CO2 Suppressor (MCS)
4
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
3 Overview of the instrument
Suppressor
5
Inline filter
7
Injection valve
9
Inline filter
11
Pulsation absorber
13
Purge valve
15
For deaerating the high-pressure pump.
Eluent degasser
17
Removes air bubbles from the eluent.
Purge valve
19
For deaerating the high-pressure pump.
Eluent degasser
21
Removes air bubbles from the eluent.
Column thermostat
6
With two column holders for two separation columns.
Sample degasser
8
Removes air bubbles from the sample.
Pulsation absorber
10
Mixing valve
12
Base tray
14
With leak sensor.
High-pressure pump
16
Pumps the eluent through the IC system.
Mixing coil
18
High-pressure pump
20
Pumps the eluent through the IC system.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
9

3.2 Rear

3.2 Rear
■■■■■■■■■■■■■■■■■■■■■■
■■■■■■■■
10
Figure 2 Rear
Bottle holder
1
Offers space for the eluent bottle and addi­tional accessories.
Drainage tubing connection
3
For connecting the drainage tubing, which guides escaped liquids away from the detec­tor chamber.
Back panel
2
Removable. Enables access to the detector chamber.
Transport locking screws
4
For securing the vacuum pump(s) when transporting the instrument. Up to two vac­uum pumps can be installed in an instru­ment. Only two transport locking screws are used if just one vacuum pump is installed.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
3 Overview of the instrument
Vacuum connection
5
For connecting an Extension Module that has a degasser but not its own vacuum pump. This connection has to be firmly sealed with a stopper when not in use.
PC connection socket
7
For connecting the instrument to the com­puter with the USB cable (6.2151.020).
Transport locking screws
9
For securing the high-pressure pump (in the middle plug-in) when transporting the instrument.
Transport locking screws
11
For securing the high-pressure pump (in the bottom plug-in) when transporting the instrument. These screws are only installed if a plug-in with a high-pressure pump is used in the bottom slot.
Leak sensor connection socket
13
Labeled Leak Sensor. For connecting the leak sensor connection cable coiled up in the base tray.
Exhaust opening
6
Labeled Exhaust. For extracting the air from the vacuum chamber.
USB connection sockets
8
Labeled USB 1 and USB 2. For connecting USB devices.
Extension Module connection socket
10
Labeled Extension Module. For connecting the cable (6.2156.060) used for connecting the instrument to the Extension Module.
Base tray
12
With leak sensor and leak sensor cable.
MSB connection sockets
14
Labeled MSB 1 and MSB 2. For connecting MSB devices.
Cooler
15
For cooling the power supply unit. May become hot!
Knurled screws
17
For fastening the removable back panel.
Power socket
16
Power socket for connecting the power cable and power switch for switching the instrument on and off.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
11

4.1 Setting up the instrument

4 Installation

4.1 Setting up the instrument

4.1.1 Packaging

The instrument is supplied in highly protective special packaging together with the separately packed accessories. Keep this packaging, as only this ensures safe transportation of the instrument.

4.1.2 Checks

Immediately after receipt, check whether the shipment has arrived com­plete and without damage by comparing it with the delivery note.

4.1.3 Location

The instrument has been developed for operation indoors and may not be used in explosive environments.
■■■■■■■■■■■■■■■■■■■■■■
Place the instrument in a location of the laboratory which is suitable for operation, free of vibrations, protected from corrosive atmosphere, and contamination by chemicals.
The instrument should be protected against excessive temperature fluctu­ations and direct sunlight.

4.2 Removing the handle

The instrument is equipped with a handle in order to make it easier to transport. The handle can be removed once the instrument is in place in the lab.
Accessories
You do not need any accessories for the following work steps.
■■■■■■■■
12
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
4 Installation
Removing the handle

4.3 Removing transport locking screws

To avoid damage to the drives for the high-pressure pump and the vac­uum pump during transport, the pumps are secured with transport lock­ing screws. These are located at the rear of the instrument and labeled with Transport security screws.
Remove these transport locking screws before the initial start-up.
Accessories
For this step you need:
Hex key 4 mm (6.2621.030)
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
13
4.3 Removing transport locking screws
Remove the transport locking screws
■■■■■■■■■■■■■■■■■■■■■■
Figure 3 Removing the transport locking screws
Transport locking screws
1
For the vacuum pump.
Transport locking screws
3
For an additional high-pressure pump in the bottom drawer.
Store the transport locking screws in a safe place. Reinsert the transport locking screws each time you transport the instrument a significant dis­tance.
The pumps may be damaged if you transport the instrument without inserting the transport locking screws.
CAUTION
Transport locking screws
2
For the high-pressure pump.
■■■■■■■■
14
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
4 Installation

4.4 Connecting the drainage tubing and leak sensor

The leak sensor detects leaking liquid that collects in the instrument's base tray. Liquid that leaks in the bottle holder or in the detector chamber is routed to the base tray using drainage tubing and is detected there.
If the leak sensor detects a leak in the IC system, the IC instrument is switched off and a warning is output in the software.
The leak sensor functions properly only if the following preconditions are met:
The drainage tubing is connected. The leak sensor connection cable is inserted into the leak sensor con-
nection socket.
The 940 Professional IC Vario is switched on. The leak sensor is switched to active in the software.

4.4.1 Installing the drainage tubing

Liquid that leaks in the bottle holder or detector chamber flows to the rear of the instrument and is drained through openings on the bottle holder and in the detector chamber. The drainage tubing has to be mounted at these openings. This drainage tubing guides the leaking liquid to the base tray where the leak sensor is located.
Accessories
For this step you need the following parts from the accessory kit: Vario/ Flex Basic (6.5000.000):
2 × silicone tubing (6.1816.020) Y connector (6.1807.010)
You also need scissors.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
15
4.4 Connecting the drainage tubing and leak sensor
Connecting the drainage tubing
■■■■■■■■■■■■■■■■■■■■■■

4.4.2 Connecting the leak sensor

Plugging in the leak sensor connection cable
The leak sensor connection cable is coiled up in the base tray.
■■■■■■■■
16
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■

4.5 Column thermostat

The column thermostat is completely connected. No installation work is required.

4.6 Connecting the eluent bottle

The eluent is aspirated out of the eluent bottle via the eluent aspiration tubing . The eluent aspiration tubing is installed on the input for the elu­ent degasser.
4 Installation
Accessories
For this step you need the following accessories:
These parts are part of the accessory kit Vario/Flex ONE (6.5000.010).
Eluent bottle (6.1608.070) The eluent bottle cap GL 45 accessory set (6.1602.160)
This accessory set contains the bottle cap, an M6 tubing nipple, an M8 tubing nipple, two O-rings and an M6 and M8 threaded stopper.
The tubing adapter for aspiration filter accessory set (6.2744.210)
This accessory set contains a filter holder, a clamping screw and tubing weighting.
An aspiration filter (6.2821.090)
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
17
4.6 Connecting the eluent bottle
■■■■■■■■■■■■■■■■■■■■■■
Connecting the eluent aspiration tubing
■■■■■■■■
18
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■

4.7 Connecting the eluent degasser

The eluent degasser is completely connected. No installation work is required.

4.8 Installing the high-pressure pump

The high-pressure pump is completely connected. No installation work is required.

4.9 Installing an inline filter

The inline filter is completely connected. No installation work is required.

4.10 Installing the pulsation absorber

4 Installation
The pulsation absorber is installed between the high-pressure pump and the injection valve. It protects the separation column from damage caused by pressure fluctuations such as those when switching the injection valve, and reduces interfering pulsations during highly sensitive measurements.
The pulsation absorber is completely connected. No installation work is required.

4.11 Injection valve

The injection valve is completely connected. No installation work is required.

4.12 Suppressor

The suppressor drive of the 940 Professional IC Vario can hold various rotors. The large rotors such as the SPM Rotor A (6.2835.000) and the MSM-HC Rotor A (6.2842.000) can be inserted directly.
Small rotors like the MSM Rotor A (6.2832.000) and the MSM-LC Rotor A (6.2844.000) must first be fitted into the adapter (6.2842.020) which can then be inserted into the suppressor housing.
A connecting piece (6.2835.010) is used for all rotors for connecting the suppressor to the IC system.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
19
4.12 Suppressor
■■■■■■■■■■■■■■■■■■■■■■
NOTE
The instruments are supplied without rotor and without adapter.
The suitable rotor and the adapter, if required, must be ordered sepa­rately.

4.12.1 Inserting the rotors

Accessories
For this step you need the following accessories:
For the suppression: MSM Rotor A (6.2832.000) or MSM-HC Rotor A
(6.2842.000) or MSM-LC Rotor A (6.2844.000)
Optional: Adapter (6.2842.020) Connecting piece (6.2835.010)
Large rotors can be directly inserted into the rotor housing.
The rotor may be destroyed during start-up if not inserted correctly.
Therefore, follow the following instructions exactly.
CAUTION
■■■■■■■■
20
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
4 Installation
Inserting large rotors
Inserting small rotors
You need the adapter (6.2842.020) in order to insert a small rotor into the suppressor drive.

4.12.2 Connecting the suppressor

The three inputs and outputs of the suppressor units, numbered with 1, 2 and 3 on the connecting piece, each have 2 fixed mounted PTFE capilla­ries.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
21
4.12 Suppressor
■■■■■■■■■■■■■■■■■■■■■■
Figure 4 Suppressor – connection capillaries
out
1
Output capillary for the eluent.
regenerant
3
Input capillary for the regeneration solution.
waste rins.
5
Output capillary for the rinsing solution; to the waste container.
Recommended installation
in
2
Input capillary for the eluent.
waste reg.
4
Output capillary for the regeneration solu­tion; to the waste container.
rinsing solution
6
Input capillary for the rinsing solution.
■■■■■■■■
22
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
Accessories
4 Installation
Alternative installation
Installing bottles with auxiliary solutions
To connect the bottles of the auxiliary solutions, you will need the follow­ing accessories:
Accessories from IC equipment: Dosino Regeneration (6.5330.190)
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
23

4.13 Metrohm CO2 Suppressor (MCS)

4.13 Metrohm CO2 Suppressor (MCS)

4.13.1 Connecting the MCS

The MCS is connected between the suppressor and the detector.
Connecting the MCS
■■■■■■■■■■■■■■■■■■■■■■
Figure 5 Connecting the MCS
Air aspiration capillary
1
For drawing in air with low CO2 content (via the CO2 adsorption cartridge).
Luer coupling (6.2744.120)
3
Mounted on the air aspiration capillary with a pressure screw (6.2744.070).
CAUTION
If the MCS is not used, the input and output must be sealed with the threaded stoppers (6.2744.220).

4.13.2 Installing adsorption cartridges

The suctioned air has to have the lowest CO2 content possible in order for the CO2 to be removed from the eluent as efficiently as possible. In order to achieve this, the air is aspirated through a CO2 adsorption cartridge (6.2837.000).
Pressure screw, short (6.2744.070)
2
Installed on the air aspiration capillary.
■■■■■■■■
24
However, the CO2 adsorption cartridge is sensitive to humidity; the CO
2
adsorption cartridge becomes clogged when wet. In order to protect the
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
CO2 adsorption cartridge, an H2O adsorption cartridge (6.2837.010) is also installed.
Accessories For this step you need the following accessories:
Adsorption cartridge holder (6.2057.080) with 4 clips, an adapter
(6.1808.190) and a piece of PVC tubing.
CO H
adsorption cartridge (6.2837.000)
2
O adsorption cartridge (6.2837.010)
2
All of the parts are located in one accessory kit: Vario/Flex SeS (6.5000.020).
Installing the adsorption cartridge
CAUTION
4 Installation
The following preparatory steps absolutely must be carried out for CO suppression to operate correctly.
Preparing the adsorption cartridges:
Prepare the H
O adsorption cartridge for use as specified in the provi-
2
ded leaflet.
Remove the protective cap on the tip of the CO
adsorption cartridge.
2
2
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
25

4.14 Installing the detector

■■■■■■■■■■■■■■■■■■■■■■
4.14 Installing the detector
The 940 Professional IC Vario provides enough space for two detectors and additional accessories in the detector chamber. The detectors are available as accessories and are supplied with separate manuals.
Placing the detector in the instrument
Follow the instructions in the chapter Inserting the detector in the manual for the detector.
Connecting the detector to the eluent path
Accessories
For this step you need the following accessories:
Pressure screw, long (6.2744.090)
■■■■■■■■
26
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■

4.15 Connecting the sample degasser (optional)

Gas bubbles in the sample lead to poor reproducibility, as the quantity of sample in the sample loop is not always the same. Therefore, we recom­mend degassing samples that contain gas before injection.
NOTE
The sample degasser does not have to be connected. We recommend only using the sample degasser if the application requires it.
The rinsing time increases by at least 2 minutes when the sample degasser is connected.
4 Installation
Accessories
For this step you need the following accessories:
2 × pressure screw, long (6.2744.090) PTFE capillary (6.1803.040)
Connecting the sample degasser
If the sample degasser is not used, the input and output must be sealed with threaded stoppers (6.2744.220).
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
CAUTION
■■■■■■■■
27

4.16 Installing the high-pressure gradient module

■■■■■■■■■■■■■■■■■■■■■■
4.16 Installing the high-pressure gradient module
The outputs of the two high-pressure pumps are connected to the injec­tion valve's eluent input using the mixing coil for high-pressure gradients (6.2758.000).
Connect the two pieces of eluent aspiration tubing to an eluent bottle (see Chapter 4.6, page 17).

4.17 Connecting the instrument to a computer

NOTE
The instrument must be switched off when being connected to a com­puter.
Accessories
For this step you need the following accessories:
USB connecting cable (6.2151.020)
■■■■■■■■
28
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
4 Installation
Connecting the USB cable

4.18 Connecting the instrument to the power supply

WARNING
The power supply unit must not get wet. Protect it from liquids.
Accessories
For this step you need the following accessories:
For Switzerland, …: Power supply cable with IEC 60320 line socket,
type C13, with SEV 1011 plug, type 12 (6.2122.020), 1.5 m
For Germany, …: Power supply cable with IEC 60320 line socket, type
C13, with CEE 7 plug, type VII (6.2122.040), 1.5 m
For the USA, …: Power supply cable with IEC 60320 line socket, type
C13, with NEMA 5-15 plug, type 498 (6.2122.070), 1.5 m
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
29

4.19 Initial start-up

■■■■■■■■■■■■■■■■■■■■■■
Connecting the power supply cable
1
Inserting the power supply cable
Insert the power supply cable into the instrument's power socket. Connect the power supply cable to the power supply.
2
Switching on the instrument
Switch on the instrument using the power switch.
After being switched on, the LED on the front of the instrument flashes. The instrument conducts a system test and establishes a con­nection to the software. Once the system test is complete and the connection to the software has been established, the LED lights up continuously.
4.19 Initial start-up
Even before the guard column and separation column are installed, the entire system has to be completely rinsed with eluent for the first time.
■■■■■■■■
30
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
4 Installation
Rinsing the IC system
CAUTION
The guard column and separation column must not be installed for the initial start-up.
Make sure that a coupling (6.2744.040) is being used instead of the columns.
1
Preparing the software
Start the MagIC Net computer program. Open the Equilibration tab in MagIC Net: Work-
place Run Equilibration.
Select (or create) a suitable method.
Also see: MagIC Net tutorial and online help.
2
Preparing the instrument
Ensure that the eluent aspiration tubing is immersed in the eluent
and there is enough eluent in the eluent bottle.
STREAM method (recommended): Ensure that the aspiration tub-
ing for the regeneration solution is immersed in the solution and that there is enough solution; also check that the detector output capillary is connected to the suppressor's input capillary for rinsing solution (labeled rinsing solution). Alternatively, if two bottles are being used for rinsing and regen­eration: Ensure that the aspiration tubings for the auxiliary solu­tions (regeneration solution and rinsing solution) are immersed in the respective solutions and that there is enough solution in both bottles.
Switch on the instrument.
MagIC Net detects the instrument and all of its modules.
3
Starting equilibration
Start the equilibration in MagIC Net: Workplace Run Equi-
libration Start HW.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
31

4.20 Connecting and rinsing the guard column

4
Deaerating the high-pressure pump
■■■■■■■■■■■■■■■■■■■■■■
Use the syringe to aspirate eluent until there are no more air
bubbles in the eluent.
5
Rinsing the instrument without columns
Rinse the instrument (without columns) with eluent for 5 minutes.
4.20 Connecting and rinsing the guard column
CAUTION
New guard columns are filled with solution and sealed with stoppers or caps on both sides.
Before inserting the guard column, ensure that this solution can be mixed with the eluent being used (follow information from the manu­facturer).
■■■■■■■■
32
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
NOTE
The guard column may not be connected until after the instrument has already been put into operation once (see Chapter 4.19, page 30). The guard column and separation column have to be replaced by a coupling (6.2744.040) until then.
Accessories For this step you need the following accessories:
Guard column (suitable for the separation column)
Connecting the guard column
4 Installation
Rinsing the guard column
1
Rinsing the guard column
Place a beaker under the guard column's output. Start manual control in MagIC Net and select the high-pressure
pump: Manual Manual control Pump
Flow: in accordance with column leafletOn
Rinse the guard column with eluent for approx. 5 minutes. Use manual control in MagIC Net to stop the high-pressure pump
again: Off.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
33

4.21 Connecting the separation column

4.21 Connecting the separation column
CAUTION
New separation columns are filled with solution and sealed with stop­pers on both sides. Before inserting the column, ensure that this solu­tion can be mixed with the eluent being used (follow information from the manufacturer).
NOTE
The separation column may not be connected until after the instrument has already been put into operation once (see Chapter 4.19, page 30). The guard column and separation column have to be replaced by a coupling (6.2744.040) until then.
■■■■■■■■■■■■■■■■■■■■■■
■■■■■■■■
34
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
4 Installation
Rinse the separation column as follows.
Connecting the separation column
1
Rinsing the separation column
Place a beaker under the separation column's outlet. Start manual control in MagIC Net and select the high-pressure
pump: Manual Manual control Pump
Flow: in accordance with column leafletOn
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
35

4.22 Conditioning

Rinse the separation column with eluent for approximately 10
Use manual control in MagIC Net to stop the high-pressure pump
4.22 Conditioning
After installation, after each time the instrument is switched on and after each time the eluent is changed, the system has to be conditioned with eluent long enough that a stable baseline is attained.
The conditioning time can lengthen considerably after changing the eluent.
Conditioning the system
■■■■■■■■■■■■■■■■■■■■■■
minutes.
again: Off.
NOTE
1
Preparing the software
CAUTION
Ensure that the configured flow is not higher than the flow permit­ted for the corresponding column (refer to the column leaflet and the chip data record).
Start the MagIC Net computer program. Open the Equilibration tab in MagIC Net: Work-
place Run Equilibration.
Select (or create) a suitable method.
Also see: MagIC Net tutorial and online help.
2
Preparing the instrument
Ensure that the column is inserted correctly in relation to the flow
direction marked on the sticker (arrow has to point in the direc­tion of flow).
Ensure that the eluent aspiration tubing is immersed in the eluent
and there is enough eluent in the eluent bottle.
3
Starting equilibration
Start the equilibration in MagIC Net: Workplace Run Equi-
libration Start HW.
■■■■■■■■
36
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
Visually inspect whether all capillaries and their connections from
the high-pressure pump to the detector are leak-tight. If eluent is leaking out anywhere, tighten the corresponding pressure screw further, or loosen the pressure screw, check the end of the capil­lary and shorten it using the capillary cutter if necessary and retighten the pressure screw.
4
Conditioning the system
Rinse the system with eluent until the required stability of the base­line is attained (normally 30 minutes).
The instrument is now ready for measuring samples.
4 Installation
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
37

5 Operation

■■■■■■■■■■■■■■■■■■■■■■
The 940 Professional IC Vario – ONE/SeS/HPG is operated solely using the MagIC Net software. You can find information on operating the software in the tutorial for MagIC Net or in the online help.
■■■■■■■■
38
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
6 Warranty (guarantee)
Metrohm guarantees that the deliveries and services it provides are free of defects in materials, design or manufacturing.
The general warranty period is 36 months (exclusions below) from the date of delivery, or 18 months in the event of continuous operation. The warranty remains valid on the condition that the servicing is provided by a service organization authorized by Metrohm at defined intervals and with a defined scope.
The warranty period for anion suppressors of the type "MSM" is 120 months from the date of delivery or 60 months in the case of continuous operation.
The warranty period for IC separation columns is 90 days after start-up.
For third-party components that are recognizable as such, the manufac­turer's warranty regulations apply.

6 Warranty (guarantee)

For instruments sold under the Metrohm NIRSystems brand, a full 16­month warranty is applicable. In the event of continuous operation, the warranty period is reduced by half.
Consumables and materials with limited storage life and glass breakage in the case of electrodes or other glass parts are excluded from the warranty.
Warranty claims cannot be asserted if the ordering party has failed to meet its payment obligations according to schedule.
During the warranty period, Metrohm undertakes either to replace free of charge or to credit the purchaser for any modules or components that can be shown to be faulty. Any transport or customs fees that may apply are the responsibility of the ordering party.
The precondition for this is that the ordering party has to specify the arti­cle number, the article designation, an adequate error description, the delivery date and (if applicable) the serial number or chip data in the Sup­port Tracker. Metrohm then decides whether a replacement or a credit note is to be issued or whether the faulty part has to be returned using the Return Material Authorization (RMA). If a replacement or credit note is issued, the ordering party undertakes to store the faulty part for at least 24 months in accordance with the current storage directives (in compli­ance with ESD guidelines) and to hold it in readiness for onsite inspection or for return shipment to Metrohm. Metrohm reserves the right to invoice the ordering party for these articles, including retroactively, in the event of noncompliance with these preconditions.
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
39
■■■■■■■■■■■■■■■■■■■■■■
The same warranty periods that are specified for a corresponding new part apply to parts that are replaced or repaired within the above-men­tioned warranty periods. However, replacement or repair of a part does not extend the warranty period of the entire system.
Deficiencies arising from circumstances that are not the responsibility of Metrohm, such as improper storage or improper use, etc., are expressly excluded from the warranty.
Metrohm also offers a 120-month spare parts availability guarantee and a 60-month PC software support warranty, calculated from the date on which the product is withdrawn from the market. The content of this war­ranty is the ability of the customer to obtain functioning spare parts or appropriate software support at market prices during the time of the war­ranty period. This does not apply for software products sold under the Metrohm NIRSystems brand.
If Metrohm AG is unable to meet this obligation due to circumstances beyond the control of Metrohm AG, then the ordering party shall be offered alternative solutions at preferential conditions.
■■■■■■■■
40
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■
7 Accessories
Up-to-date information on the scope of delivery and optional accessories for your instrument can be found on the Internet.
When you receive your new instrument, we recommend downloading the accessories list from the Internet, printing it out and keeping it together with the manual for reference purposes.
Instruments currently sold
If you do not know the article number of your instrument, proceed as fol­lows:
Downloading the accessories list

7 Accessories

NOTE
Go to the Metrohm website http://www.metrohm.com/com.
1
2
Click on .
The Search webpage will be displayed.
Enter a search term relating to the instrument into the search field
3
and click on Find.
The search results will be displayed.
In the search results, select the Devices tab (if it is not already
4
selected) and then click on the Metrohm article number of the required instrument (e.g. 2.852.0050).
The page with information pertaining to the searched article is dis­played.
Select the Parts tab.
5
The complete list of accessories with the scope of delivery and the optional accessories will be displayed.
6
Click on .
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
41
■■■■■■■■■■■■■■■■■■■■■■
The Partslists webpage will be displayed.
Select the desired output language.
7
With the article number entered, click on the command Generate
8
PDF.
The PDF file with the accessories data will be created in the language selected.
Direct access for all instruments
If you are unable to find your instrument using the search as described above, this may be due to the instrument not being sold anymore. Using the article number, you can download accessories lists for all instruments as follows:
Downloading the accessories list
Type http://partslists.metrohm.com into your Internet browser.
1
The Partslists webpage will be displayed.
Select the desired output language.
2
Enter the article number and click on the Generate PDF command.
3
The PDF file with the accessories data will be created in the language selected.
■■■■■■■■
42
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■■■■■■■■■■■■■■■

Index

Index
A
Adsorption cartridges
Connection ........................ 24
Aspiration tubing for eluent ...... 18
B
Baseline
Conditioning ...................... 37
C
Cartridges
Connection ........................ 24
Column
See also "Separation column"
........................................... 34
Column thermostat
Installation ......................... 17
Computer connection ............... 28
Conditioning ............................ 37
Connect
To computer ...................... 28
To power supply ................. 29
D
Degasser
Sample degasser ................. 27
Drainage tubing
Installation ......................... 15
E
Electrostatic charge .................... 6
Eluent
Aspirate .............................. 18
Eluent bottle
Installation ......................... 17
Equilibration ....................... 31, 36
F
Filter
See also "Inline filter" .......... 19
G
Grip
Also see "Handle" ............... 12
Guarantee ................................ 39
Guard column
Installation ......................... 32
Rinse .................................. 33
H
Handle ..................................... 12
Heating
Also see "Column thermostat"
........................................... 17
High-pressure pump
Protection .......................... 14
I
IC column
See also "Separation column"
........................................... 34
Injection valve ............................ 3
Installation ......................... 19
Inline filter ................................ 19
Installation
Column thermostat ............ 17
Drainage tubing ................. 15
Eluent bottle ....................... 17
Guard column .................... 32
Injection valve .................... 19
Leak sensor ........................ 16
MCS ................................... 24
Pulsation absorber .............. 19
Sample degasser ................. 27
Separation column ............. 34
Suppressor ......................... 19
L
Leak sensor
Installation ......................... 16
Leak-tightness .......................... 32
M
Mains voltage ............................. 5
MCS
Capillary connection ........... 24
Connection for cartridges ... 24
Installation ......................... 24
P
Power connection .............. 29, 30
Pulsation absorber
Installation ......................... 19
R
Rinse
Guard column .................... 33
Separation column ............. 35
S
Safety instructions ...................... 5
Sample degasser
Installation ......................... 27
Separation column
Installation ......................... 34
Protection ...................... 3, 19
Rinse .................................. 35
Service ....................................... 5
Suppressor
Install rotor ......................... 19
Installation ......................... 19
Suppressor drive
See also: Suppressor ........... 19
Switch on ................................. 30
T
Thermostat
Also see "Column thermostat"
........................................... 17
Transport
Grip .................................... 12
Transport locking screws .......... 13
V
Vacuum pump
Protection .......................... 14
Valve
See also "Injection valve" .... 19
W
Warranty .................................. 39
940 Professional IC Vario ONE/SeS/HPG (2.940.1440)
■■■■■■■■
43
Loading...