Leica CV ULTRA, ST ULTRA User Manual

Replacement of Xylene by Leica ST Ultra and Leica CV Ultra

Leica ST Ultra and Leica CV Ultra have been developed to replace Xylene/Toluene and conventional mounting
media in histopathology/Cytopathology laboratories.

Leica ST Ultra is used for clearing; Leica CV Ultra is used for coverslipping (mouting)

Leica ST Ultra and Leica CV Ultra both are:

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 Leica ST Ultra
 Leica CV Ultra

- Non flammable

- Odourless

- No detectable aromatics

- Non carcinogenic

- Non poisonous

- Environmentally and human friendly

There are two typical processes in a histo/cytopathology laboratory:

- Tissue Processing

- Deparaffination, Staining and Coverslipping

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 Leica ST Ultra
 Leica CV Ultra

Change to Leica ST Ultra in Tissue Processing

The general procedure for tissue processing is documented
below, although slight variations exist from one laboratory to
another.

Sequence Time in minutes Temperature °C

Formaldehyde 10 % v/v 30 40
Formaldehyde 10 % v/v 60 40
Ethanol 50 % 30 40
Ethanol 70 % 30 40
Ethanol 100 % 30 40
Ethanol 100 % 60 40
Ethanol 100 % 60 40
Ethanol 100 %* 120 40
Xylene 40 40
Xylene 80 ** 40
Paraffin 60 60
Paraffin 60 60
Paraffin 60 60
Paraffin 120 60

If Xylene is replaced by Leica ST Ultra it is important to note the following:

* More frequent refreshment of 100% Ethanol for tissue dehydration might be

necessary since xylene is partially miscible with water. Leica ST Ultra is not.

** Time of clearing should be increased by 30 minutes. Xylene penetration of

tissue is faster than penetration with Leica ST Ultra.

Performance advantage of Leica ST Ultra

Leica ST Ultra hardens fragile tissue material to a lesser
degree than Xylene (e.g. lymph nodes and testes).