Leica CV MOUNT User Manual

Leica CV Mount is a mounting medium based on
polymers of butylmethacrylate in xylene (no
monomers are present) with optimum viscosity for
automated coverslipping. Leica CV Mount has
been extensively tested on the Leica CV5030
Coverslipper and has proven to be the optimal
choice. Leica CV Mount has excellent viscosity
properties and a short hardening time and is
suitable for coverslipping both histology and
cytology slides.

Leica CV Mount

Mounting medium

Use the following suggested settings:

• Histology coverslipping: Mountant Setting - 2
Mountant Volume - 2.5

2. The dispensing nozzle is at correct height above
a slide (1.5 mm - use a slide and a nozzle tool as a
starting height gauge).

• Cytology coverslipping: Mountant Setting - 2/3
Mountant Volume - 4.5*

* This setting may need increasing when coverslipping thick

smears (> 50 µm thickness) or smears of irregular thickness.

Leica CV Mount is optimized for use in the Leica
CV5030 Coverslipper with minimum to no air voids
produced. There is no programming required for
using the Leica CV5030 Coverslipper with Leica CV
Mount, simply refer to the mountant type setting and
recommended volume as stated above.

Once the settings have been selected, prime the
mountant dispensing nozzle, place input and output
racks in position and press the „rack“ button on the
user keypad. Slides are withdrawn from the input rack
with precision and placed onto a moving platform
where Leica CV Mount is applied undiluted from an
integral mountant reservoir via an adjustable nozzle.

„Good Laboratory Practice“ will allow the user to
achieve optimum coverslipping performance. Below
is a list of hints and tips to assist the laboratory to
achieve this.

3. If the Leica CV5030 Coverslipper is to be left
unused for several hours remove the nozzle and
soak it in xylene. Otherwise clean the nozzle
externally using xylene soaked towelling, and
internally by soaking the nozzle in xylene.

4. Ensure there are no air graps or bubbles in the
tubing before running Leica CV5030 Coverslipper.
These bubbles can be removed by priming the
instrument automatically.

5. Xylene is the recommended clearing agent for
Leica CV Mount. It’s use will prevent mountant and
solvent incompatibilities. This would be observed
by appearance of cloudy or milkylooking mountant
or swirly patterns observed under the coverslip.

6. The walking beam is clear of any type of debris
and/or mountant.

7. Good quality coverslips have been checked for
broken glass fragments and sticking coverslips
removed.
Ensure coverslips are pushed to the rear of the
magazine and there are adequate coverslips in
the magazine.

1. Make sure that when first using Leica CV Mount,
or when changing over from another mountant,
the tubing system is first thoroughly flushed with
xylene. This will prevent cross-contamination
between mountants, i.e. microbubbles may be
detected on the slides if two incompatible
mountants are allowed to mix. We suggest
flushing the tubing system six times with xylene
(6 long primes), waiting 30 minutes and repeating
this procedure at least once, until solvent flows
freely from the nozzle. Then priming the tubing
system with Leica CV Mount using same
procedure as for xylene.

Published by:
Leica Microsystems Nussloch GmbH

Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany

Phone: [phone]
Fax: [phone]
eMail: [e-mail]
Internet: http://www.histo-solutions.com

8. Good quality slides are selected. Preferabely
ground glass slides from a reputable manufac­turer.

9. Slide racks must be stored correctly. Rough
handling can lead to distortion which will effect
the ability of the gripper arm to remove slides from
a rack.

“Good Laboratory Practice” and a safe working
environment is very imortant for coverslipping with
all mountants. If the above hints and tips are followed
the coverslipping results will be excellent.