Eppendorf Repeater plus User Manual

4981 900.095
Multipette® plus
Repeater® plus

Operating manual

Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner.
Trademarks
Eppendorf Combitip, Biopur are registered trademarks of Eppendorf AG,
and the Eppendorf logo, Multipette and Repeater,
Hamburg, Germany.
Registered trademarks are not marked in all cases with ™ or manual.
in this
U.S. Patent No.
5,591 408
5,620,660
5,620,661
U.S. Design Patent No. Combitip plus
384, 163
384, 162
387, 426
4981 900.095-08/012013
2
Multipette® plus · Repeater® plus — Operating manual
Operating manual — Multipette® plus · Repeater® plusseeon p.Fig.Tab.p.
Operating manual

Table of contents

1Table of contents
1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
2.1 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
4.1 Choose Combitip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
4.2 Insert Combitip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
4.3 Select volume. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
4.3.1 Filling Combitip and reverse stroke . . . . . . . . . . . . . . . . . . . . 8
4.3.2 Discard first dispensing step (reverse stroke). . . . . . . . . . . . .9
4.4 Dispense . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
4.5 Release Combitip. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.6 Quick Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
4.7 Empty Battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
5 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
5.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
6.1 Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
6.2 Change battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
6.3 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . 12
7 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
8 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
9 Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
10 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
10.1 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16

1 User instructions

1User instructions

1.1 Using this manual

Please read this operating manual completely before using the
device for the first time.
Please view this operating manual as part of the product and keep
it somewhere easily accessible.
When passing the device on to third parties, be sure to include this
operating manual.

2 Product description

2Product description

2.1 Features

In combination with Eppendorf Combitips advanced, the Multipette plus/the Repeater plus forms a dispensing system for repeat dispensing operations.
3
Multipette® plus · Repeater® plus — Operating manual
Fig. 1: Multipette plus/Repeater plus
1 Volume selection dial 2 Position indicator
3 Battery compartment 4 Digital display
5 Dispensing lever 6 Filling lever
7 Ejection keys 8 Serial number

2.2 Combitips advanced

A supply of liquid is aspirated into the Combitip advanced and then dispensed step by step.
The built-in electronics automatically recognize
the Combitip advanced inserted and
the position of the volume selection dial,
and the current dispensing volume appears in the display. Nine Combitips advanced with a variety of filling volumes are
available for single use (see Choose Combitip on p. 7). The Combitips advanced 25 mL and 50 mL can only be used in combination with an adapter. The adapter is a wear part.
For each Combitip advanced, you can use the volume selection dial to set 20 different volumes.
The smallest volume can be dispensed up to a max. of 100 times and the largest volume a max. of 5 times.
^
1=100µL
1
2
US-PAT.No. 5.620,660
34
5 mL
5
67
Fig. 2: Combitip advanced
1 Dispensing volume with the
manual dispenser at selection dial position 1
2 Graduation
3 US patent numbers 4 Maximum volume
5 Color code 6 Coding
7 Piston

2.3 Combitips plus

Multipette plus/Repeater plus can also be used with Combitips plus (predecessor model).
4
Multipette® plus · Repeater® plus — Operating manual

3Safety

3Safety

3.1 Intended use

The Multipette plus/Repeater plus is a lab device and, in combination with a Combitip plus/Combitip advanced, it is intended for dispensing aqueous solutions in the volume range between 1 μL and 10.00 mL. In vivo applications (applications in or on the human body) are not permitted.
The Multipette plus/Repeater plus may only be operated by trained specialist staff. All users must have read the operating manual carefully and familiarized themselves with the device's mode of operation.
CAUTION! Danger to persons from careless use.
Never point the opening of a Multipette plus / Repeater
CAUTION!
CAUTION!
NOTICE!
plus equipped with a Combitip at yourself or anyone else.
Only perform dispensing operations when you do not
endanger yourself or anyone else.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, func­tion and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts or from the improper use of such equipment.
Only use accessories and original spare parts
recommended by Eppendorf.
NOTICE! Damage to device from opening the housing.
Do not remove the housing of the Multipette plus/
Repeater plus.
Do not allow the housing to be opened by anyone
except service staff authorized by Eppendorf.
NOTICE! Damage to sensor from damaged or incorrectly inserted Adapter plus.
When using 25 mL and 50 mL Combitips, make sure
NOTICE!
that the adapter is in a perfect condition. The adapter is a wear part.
Do not use the Adapter if it is damaged or visibly worn. Connect the Adapter and the Combitip always outside
of the Multipette plus/Repeater plus.
Do not turn the inserted Combitip. Always hold the Multipette plus/Repeater plus by the
housing.
NOTICE! Damage to device from penetration of liquids.
Do not allow any liquids to penetrate the inside of the
NOTICE!
housing.
If liquid has penetrated the inside of the housing, have
the inner parts of the Multipette plus / Repeater plus repaired only by service partners of Eppendorf AG. Contact your responsible sales office before returning a product.
5
Multipette® plus · Repeater® plus — Operating manual
NOTICE! Material damage from incorrect use.
Only use the product for its intended purpose as
NOTICE!
described in the operating manual.
Ensure adequate material resistance when using
chemical substances.
Do not use the Multipette plus/Repeater plus with
liquids which attack the materials ABS, ASA, PBT, PC, POM and PPS.
Do not use the Combitip with liquids which attack the
materials PP, PE or LCP.
In case of doubt, contact your local Eppendorf partner.
6
Multipette® plus · Repeater® plus — Operating manual

4Operation

4Operation

4.1 Choose Combitip

Use the volume table to select volumes and Compitip.
See the safety instructions and the general illustration(see Fig. on p. 4).
Hint!
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Multipette® plus · Repeater® plus — Operating manual

4.2 Insert Combitip

NOTICE! Damage to property due to incorrect insertion of the Combitip.
For Multipette plus/Repeater plus manufactured before
NOTICE!
1. If using 25 mL or 50 mL Combitips, connect the adapter to the Combitip outside the Multipette plus/Repeater plus.
12/15/2009: Do not press the ejection keys when a Combitip is inserted.
3
2
1
®
plus
Multipette
2. Slide the filling lever right to the bottom and hold it there.
3. Push the Combitip from below into the Multipette plus / Repeater plus and, in doing so, hold the filling lever slid downwards. Or insert the Combitip and then push the filling lever downward.

4.3 Select volume

Turn the volume selection wheel until it locks into the desired
position.
The volume selection wheel has 20 positions. They are indicated with the figures 1 to 10. Positions between these numbers, and position 0.5, are indicated with a spot.
When setting small volumes (position 5 and below), the operating path of the dosage lever is reduced automati-
Hint!
cally. The dosage lever changes its position when the vol­ume selection wheel is turned.
You cannot turn the volume selection wheel beyond position 10.
Do not turn the volume selection wheel beyond 10 or 0.5. Turn the volume wheel back to its initial position.

4.3.1 Filling Combitip and reverse stroke

1. Immerse the tip of the Combitip in the liquid.
2. Slowly and uniformly push the filling lever up to the stop. The display is blinking, the Multipette plus/Repeater plus is not yet
ready for operation.
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Multipette® plus · Repeater® plus — Operating manual

4.3.2 Discard first dispensing step (reverse stroke).

1. Wipe the Combitip tip using lint-free cell tissue.
2. Place the Combitip tip on the tube wall.
3. Actuate the dispensing lever once and discard the liquid (reverse stroke).
This step, which brings the system to the basic position, should be completed independent of the volume setting.
The display is no longer blinking, and displays the dispensing volume. The Multipette plus/Repeater is ready for operation, the full range of dispensing steps are available.
Comments
If you do not fully fill the Combitip, the display will not blink. Press
the dispensing lever fully downward as often as necessary until liquid escapes from the Combitip plus. Discard the liquid. Then, you can dispense the set volume.
Between dispensing steps, you can change the volume using the
volume selection dial.
Pressing the filling lever too quickly may create a too high vacuum.
The liquid is enriched with air bubbles which could cause dispensing inaccuracies.
With a volume selection dial setting larger 5, the aspiration of a large air bubble can lead to drip formation after each dispensing step, particularly with 10, 25 and 50 mL Combitips.
A small air bubble in the upper cylinder chamber has no effect on
dispensing.
The Combilong/Combitube is an aspiration aid for the Multipette
plus/Repeater plus. The Combilong/Combitube enables direct liquid aspiration from bottles.

4.4 Dispense

1. Place the tip of the Combitip against the wall of the tube.
2. Push the dispensing lever up to the stop.
If the remaining liquid is not enough for complete dispens­ing of the set volume for the last dispensing step, the resid-
Hint!
ual stroke lock automatically prevents an insufficient amount from being dispensed.
After the Combitip has been refilled, discard the first dispensing step.

4.5 Release Combitip

1. Hold the Combitip in a tube and push the filling lever right to the bottom.
The residual liquid is emptied.
2. Hold the Multipette plus/the Repeater over a waste container.
3. Press both ejection keys at the same time. The Combitip (in the case of 25 mL and 50 mL including adapter)
is released from the Multipette plus/the Repeater plus. The display goes out.
4. On the Combitip 25 mL and 50 mL: unscrew the reusable adapter from the Combitips.
The adapter is a wear part. If significant wear is apparent, do not reuse the adapter.
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Multipette® plus · Repeater® plus — Operating manual

4.6 Quick Start

Only trained staff already familiar with the operating man­ual and the Multipette plus/the Repeater plus may work to
Hint!
these short instructions. Follow the safety instructions.
1. Select Combitip.
2. With Combitip 25, 50 mL: connect adapter to the Combitip.
3. Insert Combitip (see p. 8).
4. Fill Combitip:
a Immerse the tip of the Combitip in the liquid. b Slowly draw up the filling lever.
5. Reverse stroke:
a Press the dispensing lever. b Discard the first dispensing step (reverse stroke).
6. Dispense:
a Place the tip of the Combitip against the wall of the tube. b Press the dispensing lever.
7. Discard the Combitip:
a Push the filling lever right to the bottom. b Press both ejection keys at the same time.

4.7 Empty Battery

The Multipette plus/Repeater plus can still function if the electronics fail or the battery is empty.
To set volumes, use the volume table (see p. 7) or proceed as follows in order to determine the position required on the volume selection wheel:
1. Read the volume for position 1 on the volume wheel from the Combitip (fig. 2-1).
2. Divide the desired volume by the volume with position 1.
Examples for Combitip
Volume for position 1 Desired volume of dosage Calculation Position to be set
100 μL 200 μL 200 / 100 = 2
2
Desired volume of dosage Calculation Position to be set
Desired volume of dosage Calculation Position to be set
10
750 μL 750 / 100 = 7.5 between 7 and 8
50 μL 50 / 100 = 0.5 before 1
Multipette® plus · Repeater® plus — Operating manual
e dispensing l

5 Troubleshooting

5Troubleshooting

5.1 General errors

Should you require service, contact your official dealer for Eppendorf products or our sales office. You can find the
Hint!
addresses of our dealers on our website www.eppendorf.com are listed on the penultimate page of these Instructions for Use.
Read the note on decontamination (see Decontamination before shipment on p. 12).
. The addresses of our sales offices
11
Multipette® plus · Repeater® plus — Operating manual

6 Maintenance

6Maintenance

6.1 Cleaning

Any servicing activities above and beyond cleaning or changing the battery must be performed only by Eppen-
Hint!
dorf AG or an Eppendorf AG service partner.
NOTICE! Damage to device from penetration of liquids.
Do not allow any liquids to penetrate the inside of the
NOTICE!
Clean any dirt off the Multipette plus / Repeater plus as follows:
housing.
If liquid has penetrated the inside of the housing, have
the inner parts of the Multipette plus / Repeater plus repaired only by service partners of Eppendorf AG. Contact your responsible sales office before returning a product.
Moisten a cloth with soap solution and remove the dirt. To disinfect the Multipette plus/Repeater plus, use isopropanol
(70%).

6.2 Change battery

When the battery symbol appears in the digital display, you can use the battery for approximately another two weeks.
1. Push the lid in the direction of the arrow to open the battery compartment.
2. Remove the old battery.
3. Wipe over the new battery with a soft dry cloth.
4. Insert the battery with the plus symbol and the labeling facing upwards.
5. Close the battery compartment.
You can also use the Multipette plus/the Repeater plus without a battery (see Empty Battery on p. 10).
Hint!

6.3 Decontamination before shipment

If you wish to return the pipette to Eppendorf AG or an Eppendorf AG service partner to be checked or repaired, please note the following:
CAUTION! Use of a contaminated device may result in personal injuries and damage to the device.
Clean and decontaminate the Multipette plus/Repeater
CAUTION!
Hazardous substances are:
solutions presenting a hazard to health
potentially infectious agents
organic solvents and reagents
radioactive substances
proteins presenting a hazard to health
DNA
1. Please note the information in the document "Decontamination certificate for product returns".
You can find it as a PDF file on our homepage www.eppendorf.com
2. Enter the serial number of the Multipette plus/Repeater plus in the decontamination certificate.
3. Enclose the fully-completed decontamination certificate for returned goods with the Multipette plus / Repeater plus.
12
plus before shipping or storage according to the cleaning instructions.
.
Multipette® plus · Repeater® plus — Operating manual

7 Technical data

7Technical data
Combitip advanced
Testing volume
Error limits
Error
Systematic error
± % ± μ % ± μL
0.1 mL white
0.2 mL light blue
0.5 mL violet
1 mL yellow
2.5 mL green
5 mL blue
10 mL orange
25 mL red
50 mL light grey
2 μL ±1.6 ±0.032 ±3.0 ±0.06
20 μL ±1.0 ±0.2 ±2.0 ±0.4
4 μL ±1.3 ±0.052 ±2.0 ±0.08
40 μL ±0.8 ±0.32 ±1.5 ±0.6
10 μL ±0.9 ±0.09 ±1.5 ±0.15
100 μL ±0.8 ±0.8 ±0.6 ±0.6
20 μL ±0.9 ±0.18 ±0.9 ±0.18
200 μL ±0.6 ±1.2 ±0.4 ±0.8
50 μL ±0.8 ±0.4 ±0.8 ±0.4
500 μL ±0.5 ±2.5 ±0.3 ±1.5
100 μL ±0.6 ±0.6 ±0.6 ±0.6
1 000 μL ±0.5 ±5.0 ±0.25 ±2.5
200 μL ±0.5 ±1.0 ±0.6 ±1.2
2 000 μL ±0.5 ±10 ±0.25 ±5.0
500 μL ±0.4 ±2.0 ±0.6 ±3.0
5 000 μL ±0.3 ±15 ±0.25 ±12.5
1 000 μL ±0.3 ±3.0 ±0.5 ±5.0
10 000 μL ±0.3 ±30 ±0.3 ±30
Random error
The data is valid for the following test conditions, in accordance with ISO 8655:
Liquid Water, double distilled, degased Test temperature 20 to 25 °C, constant to ±0.5 °C
with original Eppendorf Combitips
Number of determinations 10, before dispensing, Combitip com-
pletely filled Operating temperature +4 °C to +40 °C Battery Lithium button cell 3 V
210 mAh
Service life about 2 years

8 Ordering Information

8Ordering Information
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those
CAUTION!
recommended by Eppendorf may impair the safety, func­tion and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts or from the improper use of such equipment.
Only use accessories and original spare parts
recommended by Eppendorf.
13
Multipette® plus · Repeater® plus — Operating manual

8.1 Multipette plus/Repeater plus

Order No. (International)
4981 000.019

8.1.1 Accessories Multipette plus/Repeater plus

Order No. (International)
3115 000.003 022444905
4981 201.006 4980 210.001
4981 203.009 022260596
4980 215.003 022269119

8.1.2 Combitips advanced

Order No. (International)
0030 089.405
0030 089.618 0030 089.766
0030 089.413
0030 089.626 0030 089.774
0030 089.421
0030 089.634 0030 089.782
0030 089.430
0030 089.642 0030 089.790
0030 089.448
0030 089.650 0030 089.804
0030 089.456
0030 089.669 0030 089.812
14
Order No. (North America)
022260201
Order No. (North America)
022260588 022261592
Order No. (North America)
0030089405 0030089510 0030089618
0030089413 0030089529 0030089626
0030089421 0030089537 0030089634
0030089430 0030089545 0030089642
0030089448 0030089553 0030089650
0030089456 0030089561 0030089669
Description
Multipette plus Repeater plus
Description
Pipette carousel 3115
with 6 pipette holder for Research and Reference Pipettes
Multipette plus, Repeater plus, Varipette - adapter
for pipette carousel 3115 for pipette carousel 4807 (old)
Wall mounting device for Multipette plus, Repeater plus, Vari pette
with adhesive surface
Battery
3 V
Description
Combitips advanced 0.1 mL
100 pieces Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean
Combitips advanced 0.2 mL
100 pieces Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean
Combitips advanced 0.5 mL
100 pieces Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean
Combitips advanced 1.0 mL
100 pieces Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean
Combitips advanced 2.5 mL
100 pieces Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean
Combitips advanced 5.0 mL
100 pieces Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean
Multipette® plus · Repeater® plus — Operating manual
Order No. (International)
0030 089.464
0030 089.677 0030 089.820
0030 089.472
0030 089.685 0030 089.839
0030 089.480
0030 089.693 0030 089.847
Order No. (North America)
0030089464 0030089570 0030089677
0030089472 0030089588 0030089685
0030089480 0030089596 0030089693

8.1.3 Adapter advanced

Order No. (International)
Order No. (North America)
0030 089.715 0030089715
0030 089.723 0030089723
0030 089.731 0030089731
0030 089.740 0030089740
Description
Combitips advanced 10 mL
100 pieces Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean
Combitips advanced 25 mL
100 pieces Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean
Combitips advanced 50 mL
100 pieces Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean
Description
Adapter advanced 25 mL
1 piece Eppendorf Quality
Adapter advanced 50 mL
1 piece Eppendorf Quality
Adapter advanced 25 mL
7 pieces Biopur, individually wrapped
Adapter advanced 50 mL
7 pieces Biopur, individually wrapped

8.1.4 Accessories Combitips advanced

Combitips advanced Rack
®
Combitips advanced
Fig. 3: Combitips advanced Rack
A Combitips advanced Rack is available for storing Combitips advanced ( 10 mL).
Order No. (International)
0030 059.506
Order No. (North America)
Description
Combilong
Aspirating aid for removing liquids from volumetric flasks and tall bottles 2 pieces
15
Multipette® plus · Repeater® plus — Operating manual
Order No. (International)
022261550
0030 089.758 0030089758
Eppendorf Biopur® disposable articles are guaranteed to be sterile and free from pyrogen, RNase, DNA and ATP.

9Warranty

9Warranty
In the event of claims under warranty, please contact your Eppendorf distributor.
Order No. (North America)
Description
Combitube
Aspirating aid for removing liquids from volumetric flasks and tall bottles 2 pieces
Combitips advanced Rack
1 piece Eppendorf Quality
Wear parts are excluded from the warranty. When returning the Multipette plus/Repeater plus, please follow the
instructions relating to decontamination (see Decontamination before shipment on p. 12).

10 Disposal

10Disposal

10.1 Disposal

In case the product is to be disposed of, the relevant legal regulations are to be observed.
Information on the disposal of electrical and electronic devices in the European Community:
Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).
According to these regulations, any devices supplied after August 13, 2005, in the business-to-business sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. They are marked with the following symbol to indicate this:
As disposal regulations may differ from country to country within the EU, please contact your supplier if necessary.
WARNING! Risk of explosion and fire due to overheated accumulators and batteries.
Do not heat accumulators and batteries to over 80 °C
WARNING!
and do not throw them into fires.
Disposing of accumulators and batteries
Do not dispose of accumulators and batteries as household waste. Dispose of accumulators and batter­ies according to the locally applicable legal regula­tions.
16
Multipette® plus · Repeater® plus — Operating manual
17
Your local distributor:
www.eppendorf.com/worldwide
Eppendorf AG · 22331 Hamburg · Germany
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