PatchMan NP 2
Bedienungsanleitung · Operating Manual · Mode d’emploi
Istruzioni d’impiego · Manual de Instrucciones
Inhalt / Contents / Sommaire / Indice / Indice
Bedienungsanleitung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Operating Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Mode d’emploi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
Istruzioni d’impiego . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Manual de Instrucciones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
EG-Konformitätserklärung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
EC Conformity Declaration
Déclaration de conformité
Dichiarazione di conformità CE
Declaratión de conformidad CEE
Inhalt / Contents / Sommaire / Indice / Indice
Nachdruck und Vervielfältigung – auch auszugsweise – nur mit Genehmigung.
No part of this publication may be reproduced without the prior permission of the copyright owner.
Toute reproduction, complète ou partielle et quel que soit le proèdè est interdiete, sauf autorisation
Ristampa e riproduzione – anche di estratti – solo con autorizzazione.
Reimpresión y copia – incluso parciales – sólo con autorización.
Copyright
2
expresse de notre part
©
2005 Eppendorf AG, Hamburg
Contents
1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
2 Safety precautions and application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
2.1 Hazards when unpacking and other information and instructions . . . . . . . . . . . . . . 53
3 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
3.1 Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
3.2 Module unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
3.3 Control board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
3.3.1 Tool movement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
3.3.2 Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Contents
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
4.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
4.2 Assembling the module unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
4.2.1 Components of the module unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
4.2.2 Straight-line guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
4.2.3 Assembling the X module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
4.2.4 Assembling the X head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
4.2.5 Assembling the hinged bracket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
4.3 Mounting the module on the microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
4.3.1 Right and left side mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
4.3.2 Assembling the capillary holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
4.3.3 Angle adjuster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
4.3.4 Assembling the preamplifier holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
4.3.5 Height adjustment of X module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
4.3.6 Position change in X direction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
4.3.7 Grounding of the motor module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
4.4 Connecting cables – Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
5 User control and mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
5.1 First steps. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
5.2 More information on the mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
5.2.1 Setting up the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
5.2.2 Custom adaptation of the speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
5.2.3 Storing and selecting position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
5.2.4 Capillary exchange. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
5.3 Use of the keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
5.3.1 Coarse/Fine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
5.3.2 Extra fine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
5.3.3 Setting the speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
5.3.4 Home . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
5.3.5 Pos 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
5.3.6 Axial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
5.3.7 Z only . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
5.3.8 Standby / Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
5.3.9 Joystick button / Joystick deactivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
48
Contents
5.3.10 Turn wheel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
5.4 Parameter setting via keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
5.4.1 General-Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
5.4.2 Software version polling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
5.4.3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
6 Cleaning and service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
6.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
6.2 Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
6.3 Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
8 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
9 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
9a Ordering information for USA and Canada . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
A Appendix – PC interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
A.1 Transfer parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
A.2 Command list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
A.3 Parameter list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
A.4 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Contents
49
1
Summary
1 Summary
The Eppendorf PatchMan NP 2 micromanipulator is specially designed for use in
electrophysiology, and is marked by extreme ease of operation.
This makes the PatchMan NP 2 the right device for anyone performing electrophysiological
measurements on cells and membrane pieces, as well as being suitable for use as a universal
micromanipulator in many other applications.
The PatchMan NP 2 micromanipulator combines the classic benefits of a mechanical system
(direct feeling for tool movements) with the advantages of a precise motorised system.
The direct adapter attachment of the module unit to all commonly used microscopes (optionally
positioned at the left or right) and the separate control board prevent oscillation and vibration.
The device is adapted to upright microscopes by means of the Eppendorf universal stand.
The tool can be attached to the module unit at any angle and in any orientation.
The PatchMan NP 2 micromanipulator is designed for non-damaging placement of
measurement electrodes on and in the cell. This is aided by the device's facility to approach the
cell membrane extremely slowly. In this, the virtually drift-free mechanism particularly supports
measurements which take longer than 30 minutes. Also, the joystick can be locked to prevent
accident movement. Special features include the ability to execute a purely downward
movement and the position memory.
The changing of pipettes frequently necessary in patch-clamp measurements is made much
faster and easier by the HOME function.
50
2 Safety precautions and application
It is essential to observe the following safety precautions!
• The device must be commissioned and subsequently used in compliance with the instruc-
tions set out in this Manual, in order to protect the user and prevent damage to the device!
• The device may only be used for the purpose for which it is intended!
• The device is intended for use in the research laboratory only!
• The device is to be used solely for micromanipulation in accordance with the appropriate
legislative requirements!
• Do not operate the device if there is visible damage to the device itself and/or to its
packaging.
• Before starting up the device, please compare your power supply with the voltage
requirements on the identification plate!
• Take care when transporting the control board!
Do not transport the device by carry the joystick!
• When installing and dismantling, make sure the weight of the motor module (1.4 kg) can be
safely carried! Otherwise injury may be caused, or the device may be damaged!
• A weight of max. 100 g may be applied to the motor module.
• The motor modules must not be moved against a mechanical obstacle, as this may damage
the drive units.
• Before transporting the micromanipulator, dismantle the module unit and, if possible,
transport it in its original box. The delivered transport guards are to be mounted according
to the red supplement sheet.
• Important!
The mains plug must not be plugged into the socket outlet until all connections
between the control console, power supply unit and motor module have been
made.
• Caution – risk of trapping/crushing!
Do not reach into areas of the motor modules in which your hand may become
trapped (the gap between the guide and the module or between the module
and any obstacles), and do not insert any other objects into such gaps!
• Caution when mounting tools!
Mounting must not be performed with pressure. Glass capillaries can be
damaged during mounting if they are not handled carefully. Wear eye
protection to prevent the risk of injury from glass splinters!.
• Caution when mounting tools!
When the tool is mounted, the motors are swung out forwards and the
capillaries face the front. Handle with care!
2
Safety precautions and application
51
2 Safety precautions and application
• Caution!
If the work is not carried out properly, for example if glass capillaries contact
a surface, they may smash and flying splinters may endanger eyes. You should
therefore always protect your eyes, such as by wearing goggles!
2
• Never point capillaries at persons!
Capillaries can suddenly be loosened from their capillary grips under high
pressure and become a missile.
• Electrical connections to devices not mentioned in this Manual are permitted only with the
consent of the manufacturer!
• Repairs must be carried out only by authorised Service personnel!
• The Manual, or any part of it, may be reproduced only with the written consent of
Eppendorf AG.
• Transfer
If the device is passed on to someone else, please include the instruction manual.
• Disposal
In case the product is to be disposed of, the relevant legal regulations are to be observed.
The disposal of electrical devices is regulated within the European Community by national
regulations based on EU Directive 2002/96/EC on waste electrical and electronic equipment
(WEEE).
Safety precautions and application
According to these regulations, any devices supplied after 13.08.05 in the business-tobusiness sphere, to which this product is assigned, may no longer be disposed of in
municipal or domestic waste.
They are marked with the following symbol to indicate this.
As disposal regulations within the EU may vary from country to country, please
contact your supplier if necessary.
• Liability for the functioning of the device shall in all cases be transferred to the owners or
operators if the device is serviced or repaired by persons not authorised by the Eppendorf
approved Service or if any action is performed which is not in conformance with the
designated use of the device. Eppendorf accepts no liability for damage arising from failure
to comply with the instructions set out in the Manual. Guarantee and conditions of liability of
the terms of sale and delivery of Eppendorf AG are not expanded by the above notes.
• Eppendorf AG reserves the right to make technical modifications to this device!
52
2 Safety precautions and application
2.1 Hazards when unpacking and other information and instructions
Before checking that the device has been delivered complete and installing it, please read the
following section thoroughly.
• The control board must not be carried or dragged by the joystick!
The joystick is a highly sensitive control element which transmits movement commands in
the 0.1 µm range! Always carry the unit by the housing only!
• Never place the control board upside down on the joystick
• Treat the motor modules with care!
• Never transport the motor modules while they are still assembled!
• Whenever possible, transport the motor modules in their original packaging!
• Do not tilt the assembled motor modules!
• After the warm-up phase (at least half an hour) the device is virtually drift-free.
• After switching on, wait for the initialisation process to complete before operating the joy-
stick. Completion of initialisation is indicated by an acoustic signal or by the LED returning
to its standby condition.
2
Safety precautions and application
53
The Eppendorf PatchMan NP 2 micromanipulator is made up of three components:
– Control board
3
– Module unit
– Mains power supply unit
Description
3 Description
3.1 Components
40°
20°
0
40°
20°
C
o
a
rs
F
e
in
e
E
x
tra
F
in
e
P
o
s
1
P
a
t
c
h
M
a
n
N
P
2
H
o
m
e
A
x
ia
l
Z
o
n
ly
S
ta
n
d
b
y
Fig. 1: PatchMan
NP 2 micromanipulator, without mains power supply unit
module unit for right side mounting
3.2 Module unit
The module unit can be mounted at the left or right on any commonly used microscopes by way
of an adapter.
The module unit is composed of a motor unit for Y and Z axis movement (Y/Z module) and the
motor unit for X axis movement. The height of the X axis is adjusted by way of the straight-line
guide. The pipetting angle is set manually by way of the X head with the angle graduations.
On the X module is the tool mount for the probe holder, the X head. Tools with a diameter, or
width, of 4 mm and weighing max. 100 g can be inserted, e.g. capillary holders for cell transfer.
54
3 Description
1
2
40°
20°
0
40°
20°
3
4
Fig. 2: Module unit (here right side mounted)
1 Motor unit for Y and Z axis movement (Y/Z module)
2 X module straight-line guide
3 Motor unit for X axis movement (X module)
4 X head with angle adjustment
3.3 Control board
The control board accommodates the keypad and the joystick with integral button, as well as
the turn wheel on the side. The movements performed on the joystick are transmitted to the
module unit. The movement modes, directions and speeds, as well as movement sequences,
are pre-set and triggered by way of the keypad, the turn wheel and the joystick.
Underside
The bay underneath the control board
accommodates the connector sockets for
the module unit and the mains power supply
as well as the serial port and the connection
for an optional foot switch.
Note: Do not place the control board down
resting on the joystick! When connecting
cables (section 4.4), place the control board
on its side on an even surface and secure it
against toppling over!
3
Description
55
3.3.1 Tool movement
3
Description
Note: When the Reset function is triggered, the joystick must not be moved until the reset is
complete. Completion of the reset is indicated by an acoustic signal or by the LED returning to
standby mode.
3.3.2 Keypad
The keypad can be used to make settings or initiate movements directly. Frequently used
settings can be entered by means of key combinations. The LEDs indicate the active condition
of a function.
Keys
To understand the key functions, users must have read the detailed Manual.
Some of the key functions are triggered when the keys are released.
3 Description
Moving the dynamic joysticks moves the capillaries in one plane.
The speed of the motion is determined by the speed of the
deflection movement on the joystick. Releasing the joystick stops
the movement, the joystick springs back to its neutral position.
Rotating the joystick causes the tool to move up and down.
Clockwise rotation: down
Counterclockwise rotation: up
Overlaid movements in all three coordinate directions are
possible.
Coarse/Fine
Speed selector key: Toggle between FINE and COARSE manipulation.
Display of selected mode by key LED.
56
Extra
Fine
Extra Fine
Speed mode for extremely fine movements.
3 Description
Home
Move out of working radius and return to the precise same spot.
Axial
Activate or disable axial movement.
Pos 1
Approach stored position.
Store current position by pressing and holding down the key.
Clear approached position by pressing and holding down the key.
Z only
Z only
Only up and down movement (Z direction).
Deactivates the X/Y movements.
Standby
Switches the device off or on.
The function is used as a reset function.
3
Description
Joystick button
Press once to deactivate joystick.
Prevents unwanted movement of the tool.
Press any key to reactivate.
Turn wheel
Setting the speed in the current speed mode.
Turn towards rear to increase speed.
Turn towards front to reduce speed.
Changes by one scale increment are signalled
acoustically.
57
– The packaging and the transport guards should be kept for use if the device is transported
4
– The control board must not be carried or dragged by the joystick!
– The joystick is a highly sensitive control element which transmits movement commands in
– Never place the control board upside down on the joystick!
–Treat the motor modules with care!
– Only remove the motor modules from their packaging together! Never tug on the module
Installation
– Never transport the motor modules while they are still assembled!
– Whenever possible, transport the motor modules with the transport guards in their original
– Do not tilt the assembled motor modules!
The packaging comprises two levels. The first level contains the accessories. The bottom level
contains the module unit and the control board.
Check that the standard accessories are present and complete.
The standard accessories are:
1 Control board
1 Motor module
1 Mains power supply unit
1 Mains power cable
1 X head with angle adjuster, incl. 1 screw M3x12
1 Hinged bracket
1 Set of Allen keys (metric)
2 Screws M5x16
2 Positioning aids for universal capillary holders
1 Headstage holder for preamplifiers HEKA EPC 7/EPC9 and AXON CV-4 HS-2
1 Operating manual
1 Set of spare screws
(2 x M5x16, 2 x M4x10, 2 x M3x16, 2 x M3x12)
4 Installation
4.1 Unpacking
again later!
the 0.1 µm range. Always carry the unit by the housing only!
connecting cable!
packaging!
Carefully lift the control board and the motor modules out of the packaging using both hands.
1. Loosen the
screws and
remove the
transport guard
(yellow).
58
2. Remove the colored
transport guard only
after completely
mounting the
device on the microscope!
4 Installation
4.2 Assembling the module unit
4.2.1 Components of the module unit
4
1
2
40°
20°
0
40°
20°
3
4
Fig. 3: Module unit (here right side mounted)
1 Motor unit for Y and Z axis movement (Y/Z module)
2 X module straight-line guide
3 Motor unit for X axis movement (X module)
4 X head with angle adjustment
4.2.2 Straight-line guide
The X module is assembled by bolting the straight-line guide onto the Y/Z module.
The height of the X module can be adjusted to the mounting point of the straight-line guide.
For the sake of clarity, the X module is not shown.
Example:
The straight-line guide is bolted to the Y/Z module through the bottom
hole. The X module can be height adjusted by turning the spindle.
Installation
59
4
Installation
4.2.3 Assembling the X module
4 Installation
Moving the mounting point of the straight-line guide upwards changes
the adjustable height.
Moving the straight-line guide downwards allows less steeply inclined
angles to be set for the tool.
Note: The orange fixing screw on the narrow front face of
the X module must be slackened prior to adjusting the
height and must then be re-tightened in order to ensure
reliable operation.
The straight-line guide is already bolted to the X module.
Turn the spindle as far as necessary to make the desired
mounting point accessible.
60
Set the straight-line guide with the
free hole (2) at the mount (1) of the
X/Y unit. The fitting pins must be
fitted into the holes provided. Fix
the straight-line guide to the Z/Y
module by the M4x10 cheese
head screw.
Note: Make sure you do not
1
mount the X module too low! If
you do, the motor module may
2
come to rest on the microscope
table as it moves down. This may
damage the drives!
4 Installation
4.2.4 Assembling the X head
Set the X head with the hole (2) at the
tool mount (1) of the X module. The
fitting pin must be fitted into the hole
provided. Fix the X head to the
X module by the M3x12 cheese head
screw.
4
Installation
40°
20°
0
40°
20°
4.2.5 Assembling the hinged bracket
1
2
1
1
2
Set up the hinged bracket according to the side on
which the module unit is to be mounted.
Example of left side mounting:
The hinge (2) is positioned at the left outer, aligned
upwards and to the rear.
For right side mounting, the hinge must be at the right
outer (not visible here, on the rear).
The module unit is mounted from the front onto the
hinged bracket (1).
61
Loading...