Eppendorf PatchMan NP 2 User Manual

PatchMan NP 2

Bedienungsanleitung · Operating Manual · Mode d’emploi

Istruzioni d’impiego · Manual de Instrucciones

Inhalt / Contents / Sommaire / Indice / Indice

Bedienungsanleitung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Operating Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Mode d’emploi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

Istruzioni d’impiego . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

Manual de Instrucciones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

EG-Konformitätserklärung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120

EC Conformity Declaration
Déclaration de conformité
Dichiarazione di conformità CE
Declaratión de conformidad CEE

Inhalt / Contents / Sommaire / Indice / Indice

Nachdruck und Vervielfältigung – auch auszugsweise – nur mit Genehmigung.

No part of this publication may be reproduced without the prior permission of the copyright owner.

Toute reproduction, complète ou partielle et quel que soit le proèdè est interdiete, sauf autorisation

Ristampa e riproduzione – anche di estratti – solo con autorizzazione.

Reimpresión y copia – incluso parciales – sólo con autorización.

Copyright

2

expresse de notre part

©

2005 Eppendorf AG, Hamburg

Contents

1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

2 Safety precautions and application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

2.1 Hazards when unpacking and other information and instructions . . . . . . . . . . . . . . 53

3 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

3.1 Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

3.2 Module unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

3.3 Control board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

3.3.1 Tool movement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

3.3.2 Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Contents

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

4.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

4.2 Assembling the module unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

4.2.1 Components of the module unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

4.2.2 Straight-line guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

4.2.3 Assembling the X module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

4.2.4 Assembling the X head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

4.2.5 Assembling the hinged bracket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

4.3 Mounting the module on the microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

4.3.1 Right and left side mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

4.3.2 Assembling the capillary holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

4.3.3 Angle adjuster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

4.3.4 Assembling the preamplifier holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

4.3.5 Height adjustment of X module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

4.3.6 Position change in X direction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

4.3.7 Grounding of the motor module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

4.4 Connecting cables – Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

5 User control and mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

5.1 First steps. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

5.2 More information on the mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

5.2.1 Setting up the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

5.2.2 Custom adaptation of the speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

5.2.3 Storing and selecting position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

5.2.4 Capillary exchange. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

5.3 Use of the keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

5.3.1 Coarse/Fine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

5.3.2 Extra fine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

5.3.3 Setting the speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

5.3.4 Home . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

5.3.5 Pos 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

5.3.6 Axial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

5.3.7 Z only . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

5.3.8 Standby / Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

5.3.9 Joystick button / Joystick deactivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

48

Contents

5.3.10 Turn wheel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

5.4 Parameter setting via keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

5.4.1 General-Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

5.4.2 Software version polling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

5.4.3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

6 Cleaning and service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

6.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

6.2 Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

6.3 Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

8 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

9 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

9a Ordering information for USA and Canada . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

A Appendix – PC interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

A.1 Transfer parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

A.2 Command list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

A.3 Parameter list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

A.4 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Contents

49

1

Summary

1 Summary

The Eppendorf PatchMan NP 2 micromanipulator is specially designed for use in
electrophysiology, and is marked by extreme ease of operation.

This makes the PatchMan NP 2 the right device for anyone performing electrophysiological
measurements on cells and membrane pieces, as well as being suitable for use as a universal
micromanipulator in many other applications.

The PatchMan NP 2 micromanipulator combines the classic benefits of a mechanical system
(direct feeling for tool movements) with the advantages of a precise motorised system.

The direct adapter attachment of the module unit to all commonly used microscopes (optionally
positioned at the left or right) and the separate control board prevent oscillation and vibration.
The device is adapted to upright microscopes by means of the Eppendorf universal stand.

The tool can be attached to the module unit at any angle and in any orientation.
The PatchMan NP 2 micromanipulator is designed for non-damaging placement of

measurement electrodes on and in the cell. This is aided by the device's facility to approach the
cell membrane extremely slowly. In this, the virtually drift-free mechanism particularly supports
measurements which take longer than 30 minutes. Also, the joystick can be locked to prevent
accident movement. Special features include the ability to execute a purely downward
movement and the position memory.

The changing of pipettes frequently necessary in patch-clamp measurements is made much
faster and easier by the HOME function.

50

2 Safety precautions and application

It is essential to observe the following safety precautions!

• The device must be commissioned and subsequently used in compliance with the instruc-

tions set out in this Manual, in order to protect the user and prevent damage to the device!

• The device may only be used for the purpose for which it is intended!

• The device is intended for use in the research laboratory only!

• The device is to be used solely for micromanipulation in accordance with the appropriate

legislative requirements!

• Do not operate the device if there is visible damage to the device itself and/or to its

packaging.

• Before starting up the device, please compare your power supply with the voltage

requirements on the identification plate!

• Take care when transporting the control board!

Do not transport the device by carry the joystick!

• When installing and dismantling, make sure the weight of the motor module (1.4 kg) can be

safely carried! Otherwise injury may be caused, or the device may be damaged!

• A weight of max. 100 g may be applied to the motor module.

• The motor modules must not be moved against a mechanical obstacle, as this may damage

the drive units.

• Before transporting the micromanipulator, dismantle the module unit and, if possible,

transport it in its original box. The delivered transport guards are to be mounted according
to the red supplement sheet.

• Important!

The mains plug must not be plugged into the socket outlet until all connections
between the control console, power supply unit and motor module have been
made.

• Caution – risk of trapping/crushing!

Do not reach into areas of the motor modules in which your hand may become
trapped (the gap between the guide and the module or between the module
and any obstacles), and do not insert any other objects into such gaps!

• Caution when mounting tools!

Mounting must not be performed with pressure. Glass capillaries can be
damaged during mounting if they are not handled carefully. Wear eye
protection to prevent the risk of injury from glass splinters!.

• Caution when mounting tools!

When the tool is mounted, the motors are swung out forwards and the
capillaries face the front. Handle with care!

2

Safety precautions and application

51

2 Safety precautions and application

• Caution!

If the work is not carried out properly, for example if glass capillaries contact
a surface, they may smash and flying splinters may endanger eyes. You should
therefore always protect your eyes, such as by wearing goggles!

2

• Never point capillaries at persons!

Capillaries can suddenly be loosened from their capillary grips under high
pressure and become a missile.

• Electrical connections to devices not mentioned in this Manual are permitted only with the

consent of the manufacturer!

• Repairs must be carried out only by authorised Service personnel!

• The Manual, or any part of it, may be reproduced only with the written consent of

Eppendorf AG.

• Transfer

If the device is passed on to someone else, please include the instruction manual.

• Disposal

In case the product is to be disposed of, the relevant legal regulations are to be observed.
The disposal of electrical devices is regulated within the European Community by national
regulations based on EU Directive 2002/96/EC on waste electrical and electronic equipment
(WEEE).

Safety precautions and application

According to these regulations, any devices supplied after 13.08.05 in the business-to­business sphere, to which this product is assigned, may no longer be disposed of in
municipal or domestic waste.

They are marked with the following symbol to indicate this.
As disposal regulations within the EU may vary from country to country, please

contact your supplier if necessary.

• Liability for the functioning of the device shall in all cases be transferred to the owners or

operators if the device is serviced or repaired by persons not authorised by the Eppendorf
approved Service or if any action is performed which is not in conformance with the
designated use of the device. Eppendorf accepts no liability for damage arising from failure
to comply with the instructions set out in the Manual. Guarantee and conditions of liability of
the terms of sale and delivery of Eppendorf AG are not expanded by the above notes.

• Eppendorf AG reserves the right to make technical modifications to this device!

52

2 Safety precautions and application

2.1 Hazards when unpacking and other information and instructions

Before checking that the device has been delivered complete and installing it, please read the
following section thoroughly.

• The control board must not be carried or dragged by the joystick!

The joystick is a highly sensitive control element which transmits movement commands in
the 0.1 µm range! Always carry the unit by the housing only!

• Never place the control board upside down on the joystick

• Treat the motor modules with care!

• Never transport the motor modules while they are still assembled!

• Whenever possible, transport the motor modules in their original packaging!

• Do not tilt the assembled motor modules!

• After the warm-up phase (at least half an hour) the device is virtually drift-free.

• After switching on, wait for the initialisation process to complete before operating the joy-

stick. Completion of initialisation is indicated by an acoustic signal or by the LED returning
to its standby condition.

2

Safety precautions and application

53

The Eppendorf PatchMan NP 2 micromanipulator is made up of three components:
– Control board

3

– Module unit
– Mains power supply unit

Description

3 Description

3.1 Components

40°
20°
0

40°

20°

C

o

a

rs

F

e

in

e

E

x

tra

F

in

e

P

o

s

1

P

a

t

c

h

M

a

n

N

P

2

H

o

m

e

A

x

ia

l

Z

o

n

ly

S

ta

n

d

b

y

Fig. 1: PatchMan

NP 2 micromanipulator, without mains power supply unit

module unit for right side mounting

3.2 Module unit

The module unit can be mounted at the left or right on any commonly used microscopes by way
of an adapter.

The module unit is composed of a motor unit for Y and Z axis movement (Y/Z module) and the
motor unit for X axis movement. The height of the X axis is adjusted by way of the straight-line
guide. The pipetting angle is set manually by way of the X head with the angle graduations.

On the X module is the tool mount for the probe holder, the X head. Tools with a diameter, or
width, of 4 mm and weighing max. 100 g can be inserted, e.g. capillary holders for cell transfer.

54

3 Description

1

2

40°
20°
0

40°

20°

3

4

Fig. 2: Module unit (here right side mounted)
1 Motor unit for Y and Z axis movement (Y/Z module)

2 X module straight-line guide
3 Motor unit for X axis movement (X module)
4 X head with angle adjustment

3.3 Control board

The control board accommodates the keypad and the joystick with integral button, as well as
the turn wheel on the side. The movements performed on the joystick are transmitted to the
module unit. The movement modes, directions and speeds, as well as movement sequences,
are pre-set and triggered by way of the keypad, the turn wheel and the joystick.

Underside

The bay underneath the control board
accommodates the connector sockets for
the module unit and the mains power supply
as well as the serial port and the connection
for an optional foot switch.

Note: Do not place the control board down
resting on the joystick! When connecting
cables (section 4.4), place the control board
on its side on an even surface and secure it
against toppling over!

3

Description

55

3.3.1 Tool movement

3

Description

Note: When the Reset function is triggered, the joystick must not be moved until the reset is
complete. Completion of the reset is indicated by an acoustic signal or by the LED returning to
standby mode.

3.3.2 Keypad

The keypad can be used to make settings or initiate movements directly. Frequently used
settings can be entered by means of key combinations. The LEDs indicate the active condition
of a function.

Keys

To understand the key functions, users must have read the detailed Manual.
Some of the key functions are triggered when the keys are released.

3 Description

Moving the dynamic joysticks moves the capillaries in one plane.
The speed of the motion is determined by the speed of the
deflection movement on the joystick. Releasing the joystick stops
the movement, the joystick springs back to its neutral position.

Rotating the joystick causes the tool to move up and down.
Clockwise rotation: down

Counterclockwise rotation: up

Overlaid movements in all three coordinate directions are
possible.

Coarse/Fine
Speed selector key: Toggle between FINE and COARSE manipulation.

Display of selected mode by key LED.

56

Extra

Fine

Extra Fine

Speed mode for extremely fine movements.

3 Description

Home

Move out of working radius and return to the precise same spot.

Axial

Activate or disable axial movement.

Pos 1

Approach stored position.
Store current position by pressing and holding down the key.
Clear approached position by pressing and holding down the key.

Z only

Z only

Only up and down movement (Z direction).
Deactivates the X/Y movements.

Standby

Switches the device off or on.
The function is used as a reset function.

3

Description

Joystick button

Press once to deactivate joystick.
Prevents unwanted movement of the tool.
Press any key to reactivate.

Turn wheel

Setting the speed in the current speed mode.
Turn towards rear to increase speed.
Turn towards front to reduce speed.
Changes by one scale increment are signalled
acoustically.

57

– The packaging and the transport guards should be kept for use if the device is transported

4

– The control board must not be carried or dragged by the joystick!
– The joystick is a highly sensitive control element which transmits movement commands in

– Never place the control board upside down on the joystick!
–Treat the motor modules with care!
– Only remove the motor modules from their packaging together! Never tug on the module

Installation

– Never transport the motor modules while they are still assembled!
– Whenever possible, transport the motor modules with the transport guards in their original

– Do not tilt the assembled motor modules!
The packaging comprises two levels. The first level contains the accessories. The bottom level

contains the module unit and the control board.
Check that the standard accessories are present and complete.
The standard accessories are:

1 Control board
1 Motor module
1 Mains power supply unit
1 Mains power cable
1 X head with angle adjuster, incl. 1 screw M3x12
1 Hinged bracket
1 Set of Allen keys (metric)
2 Screws M5x16
2 Positioning aids for universal capillary holders
1 Headstage holder for preamplifiers HEKA EPC 7/EPC9 and AXON CV-4 HS-2
1 Operating manual
1 Set of spare screws
(2 x M5x16, 2 x M4x10, 2 x M3x16, 2 x M3x12)

4 Installation

4.1 Unpacking

again later!

the 0.1 µm range. Always carry the unit by the housing only!

connecting cable!

packaging!

Carefully lift the control board and the motor modules out of the packaging using both hands.

1. Loosen the
screws and
remove the
transport guard
(yellow).

58

2. Remove the colored
transport guard only
after completely
mounting the
device on the micro­scope!

4 Installation

4.2 Assembling the module unit

4.2.1 Components of the module unit

4

1

2

40°
20°
0

40°

20°

3

4

Fig. 3: Module unit (here right side mounted)
1 Motor unit for Y and Z axis movement (Y/Z module)

2 X module straight-line guide
3 Motor unit for X axis movement (X module)
4 X head with angle adjustment

4.2.2 Straight-line guide

The X module is assembled by bolting the straight-line guide onto the Y/Z module.
The height of the X module can be adjusted to the mounting point of the straight-line guide.

For the sake of clarity, the X module is not shown.
Example:

The straight-line guide is bolted to the Y/Z module through the bottom
hole. The X module can be height adjusted by turning the spindle.

Installation

59

4

Installation

4.2.3 Assembling the X module

4 Installation

Moving the mounting point of the straight-line guide upwards changes
the adjustable height.

Moving the straight-line guide downwards allows less steeply inclined
angles to be set for the tool.

Note: The orange fixing screw on the narrow front face of
the X module must be slackened prior to adjusting the
height and must then be re-tightened in order to ensure
reliable operation.

The straight-line guide is already bolted to the X module.
Turn the spindle as far as necessary to make the desired
mounting point accessible.

60

Set the straight-line guide with the
free hole (2) at the mount (1) of the
X/Y unit. The fitting pins must be
fitted into the holes provided. Fix
the straight-line guide to the Z/Y
module by the M4x10 cheese
head screw.

Note: Make sure you do not

1

mount the X module too low! If
you do, the motor module may

2

come to rest on the microscope
table as it moves down. This may
damage the drives!

4 Installation

4.2.4 Assembling the X head

Set the X head with the hole (2) at the
tool mount (1) of the X module. The
fitting pin must be fitted into the hole
provided. Fix the X head to the
X module by the M3x12 cheese head
screw.

4

Installation

40°
20°
0

40°

20°

4.2.5 Assembling the hinged bracket

1

2

1

1

2

Set up the hinged bracket according to the side on
which the module unit is to be mounted.

Example of left side mounting:
The hinge (2) is positioned at the left outer, aligned
upwards and to the rear.

For right side mounting, the hinge must be at the right
outer (not visible here, on the rear).

The module unit is mounted from the front onto the
hinged bracket (1).

61

4 Installation

Opening the hinged bracket

0° – 45°

4

Installation

To open the hinged bracket beyond its normal
opening angle between 0° and 45° for mounting
purposes, push the pin on the hinge side up. The
slider is purposely hard to move. The unit is usually
only mounted once. The pin can be moved with the
4 mm Allen key.

0° – 45°

45° – 90°

The hinged bracket can then be opened to 90°. The
mounting holes on the module are then accessible.

90°

62

4 Installation

Example:
Motor module for left side mounting, rear.

The hinged bracket is opened up and joined to the
motor module by its inner inclined surface by means
of the M5x16 screw.

Note: The X module is not shown.

Close the hinged bracket.

4

Installation

Side view:
The module unit for right side mounting is mounted
on the hinge bracket. The hinge is not visible.

Left side mounting

The hinge is visible, the mounting point of the
X module faces downwards.

63

4.3.1 Right and left side mounting

4

Installation

4 Installation

4.3 Mounting the module on the microscope

The module unit mounting is dependent on the
chosen side of the microscope.

The module unit can be mounted on the most
commonly used microscopes (usually inverted
microscopes). The unit is mounted on the
microscope tripod by way of a separately
ordered microscope-specific adapter.
Mounting of the adapter is described in the
relevant mounting instructions. If a different
type of mounting is required, special adapters
will be needed.

The hinged bracket is mounted on the adapter
by one of the two longer rollers as shown in
illustration (here right side mounting; for the
sake of clarity the mounted X module is not
shown).

The M5x16 screw is supplied as part of the
PatchMan NP 2 accessory set; the roller is
a component of the microscope-specific
adapter.
The rollers are adapter-specific.

64

The module unit (here on the right) is joined to
the adapter by way of the roller with two
M5x16 screws. The PatchMan NP 2 is
mounted at the front position.

The screws are component parts of the
microscope-specific adapter.

Note: The installation is carried out according
to the planned mounting (section 5.4.3).

4 Installation

4.3.2 Assembling the capillary holder

40°
20°
0

40°

20°

1

According to the mounting mode on the left
side of the adapter.

Note: The installation is carried out according
to the planned mounting (section 5.4.3).

On the X module is the X head (1) for tool
mounting.

It is attached to the X module by one M3x12
screws as shown in the illustration.

The X head can accommodate tools with a
diameter of 4 mm, such as the universal
capillary holder (accessory FemtoJet

®

or

CellTram).

4

Installation

The universal capillary holder is inserted into
the head after the knurled screw has been
slackened, and then fixed in place by re­tightening the knurled screw.

40°

20°

0

40°
20°

65

4 Installation

To avoid having to reposition every time a capillary is changed, the
positioning aid can be mounted on the universal capillary holder so it fits
in the centre hole in the X head.

4

Alternatively, the universal capillary holder can also
be inserted in the lower mount on the X head.

Installation

4.3.3 Angle adjuster

40°
20°
0

40°

20°

The desired angle can be set by turning one of the
two knurled screws on the X head.

Note: The orange fixing screw on the narrow front
face of the X module must be slackened prior to

40°
20°
0

40°

20°

adjusting the height and must then be re-tightened in
order to ensure reliable operation.

Depending on the angle, it may be necessary to
adjust the height of the X module and/or adjust the
position in X direction.

66

4 Installation

4.3.4 Assembling the preamplifier holder

The holder for the preamplifier is attached by
two Phillips screws. The holder is mounted
by way of the holes in its centre row.

The corresponding holes on the X head are
revealed once the black knurled screw and
the pressure disc have been removed.

To vary the position of the holder, it can be
adjusted in its mounting holes.

The preamplifier is bolted onto the mounted holder. The various holes can be made accessible
by angling the X head.

For details on correct positioning of the capillary and the preamplifier refer to the relevant
sections concerning height adjustment of the X module and/or position changing in X direction.

A position change in Y direction is made by adjusting the module on the adapter (one hole
back).

Note: A grounding screw for the motor module is included with the preamplifier holder.

4.3.5 Height adjustment of X module

Note: The orange fixing screw on the narrow front

face of the X module must be slackened prior to
adjusting the height and must then be re-tightened
in order to ensure reliable operation.

The desired X module height can be set by turning

40°
20°
0

40°

20°

40°
20°
0

40°

20°

one of the two knurled screws on the straight-line
guide.
This height adjustment is applied in connection with
the angle adjustment and the capillary position.
(See sections 4.3.2 and 4.3.3).

4

Installation

67

4.3.6 Position change in X direction

Depending on the desired angle and/or the length of the tool, it may be necessary to remove the
motor module further away from the working point.

This change of position in X direction can be effected in two ways:

4

Installation

4 Installation

20°

40°

0
20°
40°

1. Change by way of mounting point
at hinged bracket:

The hinged bracket can be mounted such
that the motor module is positioned further
out or further in relative to the microscope.

Example:
The hinged bracket is mounted such that the
left side mounting is further out in this case.

2. Rotate X head on X module:
Dismantle X head and mount it at front hole

as shown in illustration.

4.3.7 Grounding of the motor module

The motor module can be grounded over the tapholes of the silver rod on the reverse side of the
motor module. A M 5 x 8 screw and a toothed disc, for example, can be used for this purpose.

68

4 Installation

4.4 Connecting cables – Startup

Note: The connections between the control board, the mains power supply unit and the motor

module must only be plugged and unplugged with the device switched off.
Note: Do not place the control board down resting on the joystick! When connecting cables,

place the control board on its side on an even surface and secure it against toppling over!
– The connection to the motor module is made via the corresponding connectors, as shown

in Figure 4.

– Check the voltage and frequency against the rating plate before connecting the mains

power!
– Use only the mains power supply unit supplied by Eppendorf!
– The cable is plugged into the mains power supply unit before being connected to the mains.

Important: The main power connection is the last one to be connected.

– Check that the connectors are firmly attached.

1

2

3

4
5

4

Installation

Fig. 4: Underside of control board
1 Connection for optional foot switch (position change) (SER2; SubD9, male)

2 Connection (SER1; SubD9, male) (not used on the PatchMan
3 Mains power supply connection
(Important: Connect only when all the other components have been connected.)
4 Connection for motor module (SubD25, female)
5 Serial port (SER0; SubD9, male) for computer (RS 232)

NP 2)

69

Coarse

Fine

Coarse

Fine

Extra

Fine

5 User control and mode of operation

Section 5.1 provides a brief summary of how the commands can be used for
micromanipulation. Section 5.2 provides additional information on the device's mode of
operation. Sections 5.3 and 5.4 set out detailed descriptions of all the commands.

5

5.1 First steps

Precondition

Prior to performing the first micromanipulation, it is assumed that there is a capillary in the
correctly positioned capillary holder (see section 4.3.2) or a correctly positioned preamplifier
with capillary (see section 4.3.4).
Other requirements: The device is properly initialised (mounting side defined, etc.).

The PatchMan NP 2 micromanipulator is switched on and is in the following "start mode": The
capillary is at the top position and to the side of the sample. The LED of the "Speed" key is lit in
FINE mode.
On the microscope table is a focussed sample, e.g. cells in a petri dish, coated with medium.

Movement towards sample

Toggle "Speed" key to COARSE mode.
The capillary is moved to the surface of the medium by pushing/rotating the

joystick in the direction of the sample. Visual monitoring of the movement.

User control and mode of operation

If the capillary touches the medium surface for example (visually monitored),
switch to FINE mode.

The capillary is moved into the beam path of the microscope by changing the
plane of the joystick. Carefully lowering the capillary by rotating the joystick to the
right brings the tip of the capillary into the focal plane (microscopic control). Adjust
the speed as necessary by way of the turn wheel.

The speed can be increased by turning the wheel on the right
hand side of the control board.
The change of speed is indicated acoustically.

For extra fine movements with extreme enlargement, the speed can be reduced
further by means of the Extra Fine mode. In this mode, the turn wheel can be used
to set five different speeds.

70

Z only

5 User control and mode of operation

By storing a position, recurring operations at the same position can be made easier.

To do this, move the capillary to the desired position and store the position by
pressing and holding down the position key.
Press and hold it down again to clear the position.

For some procedures it may be useful, for example, to prevent disturbing lateral
movements in order to ensure a controlled lowering of the capillary onto the cell
membrane. Pressing the "Z only" key disables movement in the X and Y
directions.

Home

To change capillaries, the Home function can be used. Move the capillary up
slightly by rotating the joystick to the left. Press and hold down the "Home" key.

Caution when mounting the tools!
When mounting the tool, the motors are swivelled out forwards and the capillaries
protrude forwards. Injury may result if care is not taken when handling them!

Caution!
If the work is not carried out properly, for example if glass capillaries contact a
surface, they may smash and flying splinters may endanger eyes. You should
therefore always protect your eyes, such as by wearing goggles!

Swivel out the motor module and change the capillary. Swivel the motor module back into place
and press the "Home" key . If it is the same length and in the same mounting mode, the capillary
is returned to its home position.

If necessary, the capillary can also be moved manually to the sample by moving the joystick in
the desired direction.

5

User control and mode of operation

Standby

When the operation is complete, the PatchMan
switched off by the “Standby“ key.

NP 2 micromanipulator can be

71

Extra

Fine

5 User control and mode of operation

5.2 More information on the mode of operation

5.2.1 Setting up the capillary

5

– Move the motor module to its top, outermost position.
– Mount the capillary with the capillary holder such that the tip of the capillary is

approximately 20 mm above and 20 mm outside the working point.
– Adjust the height or the position in X direction as necessary.

By means of the positioning aid (section 4.2.3) the universal capillary holder can be dismantled
and refitted in the tool mount as necessary. This does not affect the alignment.

5.2.2 Custom adaptation of the speed

(Coarse, Fine, Extra fine)

Press and hold down the key of the selected speed mode to reduce or increase
the speed at maximum joystick deflection. This is done in five stages, halving
each time. The lowest level reached is the largest, signalled by a long tone.

Alternatively, the turn wheel can be turned towards the rear (increase speed) or
front (reduce speed).

User control and mode of operation

5.2.3 Storing and selecting position

Press the "Pos 1" key to store and select a tool position. Storing and selecting the
position makes relocating the tool much easier.

To store the current tool position, press and hold down the "Pos 1" key. Any
existing stored position is overwritten. The store operation is acknowledged by an
acoustic signal.

Tapping the "Pos 1" key causes the tool to move to the stored position. The default speed of
this movement is 5 000 µm/s. If no position is stored a short acoustic warning signal is
delivered.

To clear the stored position, move to the position and press and hold down the position key.
Alternatively, the position can also be cleared by way of the reset function.

If the capillary is not at the stored position, press and hold down the key to overwrite the
position.

72

5 User control and mode of operation

5.2.4 Capillary exchange

Before swivelling it out, make sure the capillary cannot be impeded by any obstacles!
Wear goggles to protect against possible glass splinters! Swivel out module unit on
Y/Z module!

Swivel module unit for capillary exchange out of working radius. The capillary swivels out
forwards and upwards with the module unit.

– Before changing the capillary, bring the capillary to a position over the petri dish either

manually or by way of the Home function.
– Grip module unit at rear motor unit (Y/Z module) and swivel out.
– Do not use the X module!
– Exchange capillaries and swivel module unit back into place.
– Bring the new capillary back into the focal plane.

40°
20°
0

40°

20°

5

BUser control and mode of operation

Fig. 5: Module unit in working position and swivelled out

(here without capillary holder/preamplifier)

73

Extra

Fine

5 User control and mode of operation

5.3 Use of the keypad

The keypad can be used to make settings or initiate movements directly. Frequently used
settings can be entered by means of key combinations. The LEDs indicate the active condition

5

of a function.

Coarse

Fine

Extra
Fine

Pos 1 Z only

PatchMan NP 2

Some of the key functions are triggered when the keys are released.

User control and mode of operation

5.3.1 Coarse/Fine

Speed selector key:
Toggle between FINE and COARSE manipulation.

Display of selected mode by key LED.

5.3.2 Extra fine

Activates Extra Fine speed mode.
Can be selected irrespective of Coarse or Fine. Quit Extra Fine mode by pressing
the "Speed" key. The program switches to Fine mode.

Home

Axial

Standby

74

5 User control and mode of operation

5.3.3 Setting the speed

The speed can be set in two ways:
Directly using the turn wheel or by a long key press.

Turn wheel

The turn wheel can be used to make speed settings directly.
Turn wheel backwards: increase speed to maximum possible.

Turn wheel backwards: reduce speed to minimum possible.

The setting is made in stages.

Press and hold down the "Speed" key

The speed setting is activated by pressing and holding down the "Speed" or "Extra fine" key in
the selected speed mode (Coarse/Fine/Extra Fine). The speed is reduced in stages.

Each stage is indicated acoustically. If you switch directly from the minimum to the maximum
radius a long acoustic signal sounds. There are five different levels, each roughly halving the
speed.

The Coarse levels begin at maximum speed. Fine at 1,000 µm/s, and Extra Fine at 100 µm/s.

5

User control and mode of operation

75

5 User control and mode of operation

5.3.4 Home

The tools moves axially out of the working radius.
The function is intended for changing capillaries, or for quick removal of capillaries
out of the working radius.

5

HOME: Press (and hold down as necessary) to move out as far as motors' end position.
The red LED flashes during the movement, and is then lit steadily when the end position is
reached.
Press the key again to move the tool back to its starting point. To return the tool manually the
joystick can be moved directly. This terminates the Home function.

Clean moves the capillary out of the medium. In the process, cell particles adhering to the
medium boundary layer are removed from the capillary.

When Clean is active:
CLEAN: Tap to move out a pre-set distance.
The red LED flashes during the movement and at the end point.
Press the key again to move the tool back precisely to its starting position, or move the tool
manually.

5.3.5 Pos 1

Approach stored position.

User control and mode of operation

The current position of the tool is stored in the three spatial coordinates. Storing new positions
overwrites existing ones. Positions can also be cleared by performing a reset ("Standby" key).

Store current position by pressing and holding down the key (acoustic
acknowledgement).

Clear current position by pressing and holding down the key again (acoustic
acknowledgement).

5.3.6 Axial

Activate or disable axial movement.
Rotating the joystick causes the tool to move axially up or down. The active
function is shown by the lit LED.

Rotate clockwise: move diagonally downwards
Rotate counterclockwise: move diagonally upwards

Setting the axial angle

After pressing the key for a long time, the angle of the axial movement can be changed.
The angle can be decreased at steps of 15°.
The following values can be set: 75°, 60°, 45°, 30°, 15°.
The acoustic signal for 75° is long whereas a double beep signals the factory preset value
of 45°. The other values are acknowledged by a normal beep.

76

Z only

5 User control and mode of operation

5.3.7 Z only

Only up and down movement (Z direction).
Deactivates movement on the X and Y axes (right, left, forward and back),
only movements in Z direction (up, down) are possible.
When active the LED is lit. Deactivating enables the axes once again. The LED
goes out.

5

5.3.8 Standby / Reset

Switches the device off or on.
To switch on, press the "Standby" key again. The device runs through the

power-up routine.
This functionality is also used as a reset function.

5.3.9 Joystick button / Joystick deactivation

Pressing the joystick deactivates the joystick, so unintentional moving of the
joystick does not move the tool.
The joystick is reactivated by pressing any key.

5.3.10 Turn wheel

Setting the speed in the current speed mode.
Acoustic signalling of change of speed.

User control and mode of operation

77

5 User control and mode of operation

5.4 Parameter setting via keys

In addition to the speed setting (press and hold down "Speed" key) and position storage key
functions, there are also other special functions operated via the keypad.

5

5.4.1 General-Reset

The general reset resets the device to its delivery defaults. All the parameters, including the
installation parameters, are reset to their factory defaults.

Execution:
– Switch device to Standby.
–Press "Standby" to switch device on again and then immediately press and release "Home"

key.
The device runs through a lengthy initialisation phase.

5.4.2 Software version polling

As a software version polling facility, the version number can be indicated acoustically.
The version number is given in three signal sequences, with the digit 0 represented as 10.

Execution:
– Switch device to Standby

User control and mode of operation

–Press "Standby" to switch device on again and then immediately press and release "Axial"

key.
The device runs through the initialisation phase and the acoustic output begins.

78

5 User control and mode of operation

5.4.3 Installation

The installation is carried out by means of keypad inputs.
Execution:

– Device is on.
–Press "Speed" (Coarse/Fine) and "Axial" keys simultaneously.

The Installation subfunction is activated and is indicated by the two LEDs on the "Speed"

key being lit.

Coarse

Fine

– The "Home" key is used to enter right or left side mounting. A RIGHT setting is

acknowledged by a short acoustic signal and a LEFT setting by a long one.
–Press the "Coarse/Fine" key to terminate installation and save the parameters directly.

The device switches to routine mode and only the LED of the selected speed range is lit.
Additional user control options which should only be utilised under certain preconditions by way

of the subfunction:
– Inverting the Y axis: The Invert Y parameter can be used to invert the direction of movement

of the Y axis. This is useful for microscopes that show a mirrored image, e.g. upright micro-

scopes. Press the "Extra fine" key . The "Normal" setting is acknowledged with a short beep

or the "Inverted" setting with a long beep.
– The "Pos 1" key is used to enter horizontal or upright mounting of the Y/Z module. A UP

(upright) setting is indicated by a short acoustic signal and a FLAT (horizontal) setting by a

long one. At present the PatchMan NP 2 can only be mounted in the UP setting.
–Pressing and holding down the "Axial" key enters the mounting angle. The change of angle

per increment of 5 is indicated by a short acoustic signal, a 90 setting by a long acoustic

signal and a 45 setting by a double signal. At present only the default 0° is factory set.
–Pressing and holding down the "Pos 1" key initiates loading of a software update. To be

undertaken only by Service!

Coarse

Fine

Axial

5

User control and mode of operation

79

6 Cleaning and service

6.1 Cleaning

Before cleaning the PatchMan NP 2, disconnect the plug.

6

Ensure that no fluids enter into the inside of the micromanipulator in order to avoid short-circuits
in the electrical installation as well as corrosion.

Wipe painted parts and aluminum surfaces using a cloth and mild detergent and then with a dry
cloth.

Warning

Clean the PatchMan

Wipe every part of the PatchMan NP 2, including accessories and connector cables, with an
alcohol-based disinfectant (Isopropanol, Alcohol).

Care and maintenance

Spray disinfection is not advisable, as disinfectants can enter into the micromanipulator.

The disinfection method used must comply with current disinfection regulations and guidelines.

A decontamination certificate must accompany the PatchMan NP2.

A printed form ("Decontamination certificate for return of goods" and general instructions for
decontamination are available at our www.eppendorf.com homepage.

The serial number of the device must be entered in the decontamination certificate.

: Do not use any corrosive, solvent or abrasive detergents or polishes.

6.2 Disinfection

6.3 Decontamination prior to shipping

If the device is to be inspected or repaired by the service partners of the Eppendorf AG,
it must be free of hazardous substances and clean!

NP 2 before disinfecting.

6.4 Service

The dealer is responsible for warranty and service.

80

7 Troubleshooting

The following error situations are conceivable:

Remedy

– Motor axes move in wrong direction or do not

match joystick movement.

– Some user actions are not possible. – Perform a reset or general reset and

– Device not responding to key presses,

Home function still active.

–Tool does not move forwards or back or

forward/back (in X and Y direction) when
joystick is moved, only to side.

– When connected, no LED is lit on the device

and the device will not switch on

– An error message or warning in the form of

an 8-tone acoustic signal is delivered.

– Check installation matches motor

module setup, e.g. left side mounting
etc.

then reinstall the device (section

5.4.3).

– Move joystick or press "Home" key

again,
tool moves downwards.

– Deactivate Z only function.

– Reset, General-Reset,

Unplug mains power cable.
Check mains cable and plug.
If mains power supply unit defective:
Service

– Check all cable connections.

Rest, General-Reset,
Unplug mains power cable.
If the problem has not been
eliminated, or if it recurs: Service

7

Troubleshooting

81

8 Technical data

Power supply

Input: 120 – 240 V AC; 50 – 60 Hz; < 50 W
Output: 15 – 18 V DC; 25 W

8

Control board 15 – 18 V DC; 25 W
Contamination level: II
Overvoltage category: II (IEC 61010-1)
Pollution degree: 2 (IEC 664)
Ambient temperature: max. 40 °C
Ambient relative humidity: max. 80 %

Weights

Control board with power supply: 2.7 kg

Technical data

Module unit: 1.4 kg, incl. guide

Dimensions

Y/Z module: 120 x 100 x 68 mm
X module: 120 x 60 x 50 mm
Control board: 270 x 205 x 160 mm

Max. travel: > 18 mm (each axis)
Resolution per microstep: approx. 40 nm
Max. tool travel speed: 7,500 µm/s

The device carries the mark.

Technical specifications subject to change!

82

9 Ordering information

Order no.

PatchMan NP 2, Europe 5183 000.014
PatchMan NP 2, USA and Canada 5183 000.022
PatchMan NP 2, UK/Hongkong 5183 000.030
PatchMan NP 2, Japan 5183 000.049
PatchMan NP 2, Australia 5183 000.073
PatchMan NP 2, China 5183 000.081

Delivery package

1 Control board
1 Motor module
1 Mains power supply unit
1 Mains power cable
1 X head with angle adjuster, incl. 1 screw M3x12
1 Hinged bracket
1 Set of Allen keys (metric)
2 Screws M5x16
2 Positioning aids for universal capillary holders 5171 130.001
1 Headstage holder for preamplifiers HEKA EPC 7/EPC9

and AXON CV-4 HS-2
1 Operating manual 5183 900.015
1 Set of spare screws 2 x M5x16, 2 x M4x10, 2 x M3x16, 2 x M3x12

5173 071 002

9

Ordering information

Adapters

Adapter for Leica DMIL 5181 201.004
Adapter for Leica DMIRE2 5181 202.000
Adapter for Leica DMI 6000 5181 203.007
Adapter for Nikon TMD 5181 210.003
Adapter for Nikon TS-100 5181 212.006
Adapter for Nikon TE 200/300/2000 5181 214.009
Adapter for Olympus IX 50/70 IX 51/71/81 5181 220.009
Adapter for Olympus IMT-2 5181 221.005
Adapter for Olympus CK 30/40 / CKX 31/41 5181 222.001
Adapter for Zeiss 100/135/100 S 5181 230.004
Adapter for Zeiss 10/35 5181 233.003
Adapter für Zeiss 200, incl. CO
Adapter for Zeiss 25/25C / 40C/40CFL 5181 237.009
Hund Wilovert 30 5181 234.000
Universal stand 5181 250.005
Spacer for usage of Universal Stand on stereo microscopes

with extended dimensions
Extension plate 5181 236.002

chamber 5181 235.006

2

5181 255.007

83

9 Ordering information

Accessories (non-standard) Order no.

Universal capillary holder for accommodating Femtotips®,
Femtotips® II or grip heads

9

Grip head 0 for universal capillary holder
for microcapillaries with an outer diameter of 1.0 to 1.1 mm

Grip head 1 for universal capillary holder
for microcapillaries with an outer diameter of 1.2 to 1.3 mm

Grip head 2 for universal capillary holder
for microcapillaries with an outer diameter of 1.4 to 1.5 mm

Grip head 3 for universal capillary holder
for microcapillaries with an outer diameter of 0.7 to 0.9 mm

O-Ring Set
für grip heads 0 – 3 with removal tool

Service kit for grip heads and capillary holder,

Ordering information

consisting of O-Ring Set, adapter for Femtotips® and clamping piece

20 Femtotips

20 Femtotips® II 5242 957.000

200 Microloader 5242 956.003

Filling stand for Femtotips

Interface cable 5246/5242 5181 150 060

Foot control 5181 5181 150.051

Arc guide 5181 150.019

"A" head (used with arc guide) 5181 150.027

PC cable update 5181 150.094

Service kit
(Screws M3x8, M3x16, M4x10, M4x14, M5x16 and war. M4, M5, M8)

Straight-line guide to connect X module to Y/Z module 5181 150.035

"X" head with angle adjuster 5181 150.043

5176 190.002

5176 210.003

5176 212.006

5176 214.009

5176 207.002

5176 196.000

5176 195.004

®

®

5242 952.008

5242 009.012

5181 150.310

Cell transfer

CellTram Oil
manual piston pump for transferring cells (sperms, ES cells) and
as manual injector with universal capillary holder for
capillaries with an outer diameter of 1 mm

CellTram vario
manual piston pump with variable transmission (1:1, 1:10)
to transfer cells (sperms, ES cells) and as manual injector with
universal capillary holder for capillaries with an outer diameter of 1 mm

CellTram Air
manual piston pump for holding cells (e.g. oocytes) with
universal capillary holder for capillaries with an outer diameter of 1 mm

84

5176 000.025

5176 000.033

5176 000.017

9 Ordering information

25 VacuTips
sterile glass capillaries for holding cells, inner diameter 15 µm,
outer diameter 100 µm, 35° angle

25 TransferTips®-F (ICSI)
sterile glass capillaries for transferring sperm in the ICSI technique,
inner diameter 4 µm, outer diameter 7 µm, with spike, 35° angle,
flexible flange

25 TransferTips®-R (ICSI)
sterile glass capillaries for transferring sperm, inner diameter 4 µm,
outer diameter 7 µm, with spike, 35° angle, rigid flange

25 TransferTips®-RP (ICSI)
sterile glass capillaries for transferring sperm, inner diameter 4 µm,
outer diameter 7 µm, with spike, 35° angle, rigid, parallel flange

25 TransferTips® (ES)
sterile glass capillaries for transferring ES cells, inner diameter 15 µm,
outer diameter 20 µm, with spike, 20° angle

Order no.

5175 108.000

9

5175 106.008

Ordering information

5175 113.004

5175 114.007

5175 107.004

Microinjection

FemtoJet
Programmable microinjector with integrated pressure supply

Version Europe 5247 000.013

Version USA and Canada 5247 000.056

Version UK 5247 000.030

Version Japan 100 V, 50 – 60 Hz 5247 000.048

Version Australia 5247 000.072

Version China 5247 000.080

FemtoJet® express
Programmable microinjector incl. connection for external pressure supply

Version Europe 5248 000.017

Version USA and Canada 5248 000.050

Version UK/Hongkong 5248 000.033

Version Japan 5248 000.041

Version Australia 5248 000.076

Version China 5248 000.084

®

85

9a Ordering information for North America

PatchMan NP 2, USA and Canada 920000037
PatchMan NP 2, Europe 5183 000.014
PatchMan NP 2, UK/Hongkong 5183 000.030

9a

PatchMan NP 2, Japan 5183 000.049
PatchMan NP 2, Australia 5183 000.073
PatchMan NP 2, China 5183 000.081

Delivery package

1 Control board
1 Motor module
1 Mains power supply unit
1 Mains power cable
1 X head with angle adjuster, incl. 1 screw M3x12
1 Hinged bracket
1 Set of Allen keys (metric)
2 Screws M5x16
2 Positioning aids for universal capillary holders 920005829
1 Headstage holder for preamplifiers HEKA EPC 7/EPC9

and AXON CV-4 HS-2
1 Operating manual 5183 900.015
1 Set of spare screws 2 x M5x16, 2 x M4x10, 2 x M3x16, 2 x M3x12

Ordering information for USA and Canada

Adapters

Adapter for Leica DMIL 920008500
Adapter for Leica DMIRE2 920008526
Adapter for Leica DMI 6000 920008496
Adapter for Nikon TMD 920008534
Adapter for Nikon TS-100 920008551
Adapter for Nikon TE 200/300/2000 920008658
Adapter for Olympus IX 50/70 IX 51/71/81 920008585
Adapter for Olympus IMT-2 920008577
Adapter for Olympus CK 30/40 / CKX 31/41 920008569
Adapter for Zeiss 100/135/100 S 920008607
Adapter for Zeiss 10/35 920008593
Adapter für Zeiss 200, incl. CO
Adapter for Zeiss 25/25C / 40C/40CFL 920008666
Hund Wilovert 30 5181 234.000
Universal stand 920008631
Spacer for usage of Universal Stand on stereo microscopes

with extended dimensions
Extension plate 920008674

86

chamber 920008615

2

Order no.

920003168

920008666

9a Ordering information for North America

Accessories (non-standard) Order no.

Universal capillary holder for accommodating Femtotips®,
Femtotips® II or grip heads

Grip head 0 for universal capillary holder
for microcapillaries with an outer diameter of 1.0 to 1.1 mm

Grip head 1 for universal capillary holder
for microcapillaries with an outer diameter of 1.2 to 1.3 mm

Grip head 2 for universal capillary holder
for microcapillaries with an outer diameter of 1.4 to 1.5 mm

Grip head 3 for universal capillary holder
for microcapillaries with an outer diameter of 0.7 to 0.9 mm

O-Ring Set
für grip heads 0 – 3 with removal tool

Service kit for grip heads and capillary holder,
consisting of O-Ring Set, adapter for Femtotips® and clamping piece

20 Femtotips

20 Femtotips® II 930000043

200 Microloader 930001007

Filling stand for Femtotips

Interface cable 5246/5242 920005853

Foot control 5181 920005799

Curved guide 920005802

"A" head (used with curved guide) 920005748

PC cable update 920005837

Service kit
(Screws M3x8, M3x16, M4x10, M4x14, M5x16 and war. M4, M5, M8)

Straight-line guide to connect X module to Y/Z module 920005756

"X" head with angle adjuster 920005764

®

®

920007392

920007414

910012509

910012517

920007406

920005870

920005888

930000035

930000051

920005781

9a

Ordering information for USA and Canada

Cell transfer

CellTram Oil
manual piston pump for transferring cells (sperms, ES cells) and
as manual injector with universal capillary holder for
capillaries with an outer diameter of 1 mm

CellTram vario
manual piston pump with variable transmission (1:1, 1:10)
to transfer cells (sperms, ES cells) and as manual injector with
universal capillary holder for capillaries with an outer diameter of 1 mm

CellTram Air
manual piston pump for holding cells (e.g. oocytes) with
universal capillary holder for capillaries with an outer diameter of 1 mm

920002030

920002111

920002021

87

9a Ordering information for North America

25 VacuTips
sterile glass capillaries for holding cells, inner diameter 15 µm,
outer diameter 100 µm, 35° angle

9a

25 TransferTips®-F (ICSI)
sterile glass capillaries for transferring sperm in the ICSI technique,
inner diameter 4 µm, outer diameter 7 µm, with spike, 35° angle,
flexible flange

25 TransferTips®-R (ICSI)
sterile glass capillaries for transferring sperm, inner diameter 4 µm,
outer diameter 7 µm, with spike, 35° angle, rigid flange

25 TransferTips®-RP (ICSI)
sterile glass capillaries for transferring sperm, inner diameter 4 µm,
outer diameter 7 µm, with spike, 35° angle, rigid, parallel flange

25 TransferTips® (ES)
sterile glass capillaries for transferring ES cells, inner diameter 15 µm,
outer diameter 20 µm, with spike, 20° angle

Order no.

930001015

930001031

930001066

930001074

930001040

Microinjection

FemtoJet
Programmable microinjector with integrated pressure supply

Version USA and Canada 920010504

Version Europe 5247 000.013

Ordering information for USA and Canada

Version UK 5247 000.030

Version Japan 100 V, 50 – 60 Hz 5247 000.048

Version Australia 5247 000.072

Version China 5247 000.080

FemtoJet® express
Programmable microinjector incl. connection for external pressure supply

Version USA and Canada 5248 000.050

Version Europe 5248 000.017

Version UK/Hongkong 5248 000.033

Version Japan 5248 000.041

Version Australia 5248 000.076

Version China 5248 000.084

88

®

A Appendix – PC interface

A1 Overview

It is easy to control the PatchMan NP 2 from a Windows®* computer if the following points are
observed:

• Connection of the RS232 interfaces on the PC and the control panel using a zero modem

cable.

• The RS 232 data port must only be connected to devices conforming to the IEC 950/EN

60950 (UL1950) standards.

• Serial communication with the following parameters

Data rate: 19,200 Bits/s,

ASCII asynchronous,

8 data bits,

1 stop bit,

No parity

Local echo on
External control is possible from any program that can send ASCII characters to an interface

configured in this way . The "Hyper Terminal" program supplied with Windows®* can be used for
this purpose.

* Windows® (registered trademark of Microsoft Corporation)

A2 Installation

2.1 Hardware and cables

An RS232 interface is used to connect a PC. There a 9-pin SUBMIN D plug on the manipulator.
The PC can be connected with any zero modem cable. Mechanically, the best cable is a cable
with an angle plug housing, e.g. Eppendorf cable 5181 150.094 or 5325 620.007. At least the
TXD, RXD and GND wires must be connected. TXD and RXD (pin 2 and pin 3) are reversed in
the cable. The handshake wires are not connected in the manipulator and can therefore be
wired in any manner in the cable.

A

PC interface

2.2 Command structure

Every command consists of the actual command itself and any necessary parameters.
(See Operating Manual, Appendix B or A: PC interface)

Examples:
C001 = version request → response from the micro-manipulator: A001 5181 V1.08

C007 = GOTO position in micrometers,
The parameters for the x = 100, y = -200, z = +300 positions and for the speeds (with reference
to the axis) are thus added:
C007 100-200+300 1000 1000 1000

Note on coordinate directions:
X to the right = positive, Y: backwards = positive, Z: downwards = positive

89

A Appendix – PC interface

The command and the parameters must be separated by separators. These can be either
spaces or signs. Commas, etc. are not permissible as separators. If a space is used as a
separator, the subsequent number is regarded as positive.

A

With the exception of the block end marks, only displayable ASCII characters are used.
The block end marks can be received as CR or LF or CR/LF. CR is always sent.
All command/response codes (e.g. C010) consist of a string without any internal space.

Typing errors should be avoided in the command. If typing errors are corrected during typing,
these corrections are also transmitted, thus triggering an error message.

2.3 Communication using Hyper Terminal from Windows

Settings for parameters in Hyper Terminal:

PC interface

19200 bits/s
Databits: 8
Parity: None.
Stop bits: 1
Set flow control to "none"

ASCII set-up:
Activate line feed and local echo
Line delay: Can remain set to 0 for manual input of single commands
To process stack files, enter appropriate value (e.g. 2500 milliseconds)
Activate "Append line feeds"

Communication can be tested once the PC and the manipulator have been connected as
described above and the Hyper Terminal has been appropriately configured.

Type "C001"<CR>(Enter key) to test data transfer.
Response from the micro-manipulator: A001 5181 V1.04<CR>.
This response comes immediately. There is no change in the routine display of the micro­manipulator.

Further commands can be entered using the keyboard. For external control, remote control
should first be switched on using C004.
Command sequences can also be combined using a text editor.
To ensure that the manipulator has enough time to process one command before the next one
is received, a "line delay" should be set in Hyper Terminal.

2.4 Command list

All axis specifications relate directly to the three drive motors, regar dless of the drive's mounting
form. Coordinate transformations based on differing modes of mounting are not listed here.

®

C0xx: Command to PatchMan
A0xx: Checkback from PatchMan® NP 2

NP 2

®*

C001 Version polling Parameter: None
A001 Version reply Parameter: Version

90

A Appendix – PC interface

The control board device type and the version number of the software installed in the control
board are returned as the reply. This command can be executed at any time.

C002 General reset Parameter: None
A002 Reset about to be performed Parameter: None
The command is acknowledged and 100 ms later a general reset is performed.
This command can only be executed when the remote control is operative.

C003 Reset motor control Parameter: None
A003 Reset motor control executed Parameter: None
The motor control is reset to its default condition. This aborts the motor movement and resets
the current coordinates to 0,0,0. Each drive may have an offset of up to 10 µm. This command
can be executed at any time.

C004 Switch to remote control Parameter: None
A004 Ready for remote control commands Parameter: None
All current movements are stopped and local joystick and keypad operation is disabled. The
checkback is sent as soon as all motors are stopped and positioning is possible via the serial
port. This command can be executed at any time.

C005 Switch to manual control Parameter: None
A005 Ready for manual control Parameter: None
All current movements are stopped and local joystick and keypad operation is enabled. The
checkback is sent as soon as all motors are stopped and positioning is possible via the joystick.
This command can be executed at any time.

A

PC interface

C006 GOTO position in microsteps Parameter: px py pz vx vy vz
A006 All motors stopped Parameter: End position
The manipulator moves on all three axes at the pre-set speed to the pre-set position. To specify
no movement on one axis, the speed 0 is transmitted. The checkback is sent as soon as all
motors are stopped because either the target position has been reached or a limit switch has
been tripped. The reply indicates the status of the limit switches. If the positioning was
successful this value is 0. All entries are in microsteps. This command can only be executed
when the remote control is operative.

C007 GOTO position in micrometres Parameter: Px Py Pz Vx Vy Vz
A007 All motors stopped Parameter: End position
The manipulator moves on all three axes at the pre-set speed to the pre-set position. To specify
no movement on one axis, the speed 0 is transmitted. The checkback is sent as soon as all
motors are stopped because either the destination position has been reached or a limit switch
has been tripped. The reply indicates the status of the limit switches. If the positioning was
successful this value is 0. All entries are in micrometres.
This command can only be executed when the remote control is operative.

91

C008 STOP Parameter: None
A008 All motors stopped Parameter: None
A current movement is stopped. The checkback is sent as soon as all motors are stopped.
This command can only be executed when the remote control is operative.

A

C009 Position polling in microsteps Parameter: None
A009 Position reply in microsteps with limit switches Parameter: px py pz End
position
Regardless of any current movement, the current position on all three axes in microsteps is
returned in the reply, as is the position of the six limit switches. A current movement is not
stopped.
This command can be executed at any time.

PC interface

C010 Position polling in micrometres Parameter: None
A010 Position reply in micrometres with limit switches Parameter: Px Py Pz End
position
Regardless of any current movement, the current position on all three axes in micrometres is
returned in the reply, as is the position of the six limit switches. A current movement is not
stopped.
This command can be executed at any time.

C011 GOTO position in micro-steps without reached message Parameters: px py pz vx vy vz
A011 Ready for next command Parameters: None.
The manipulator moves in all three axes at the specified speed to the specified position. If no

movement is required in one of the axes, then its speed is sent as 0. An acknowledgement is
sent as soon as the command has been fully evaluated and the next one can be sent. The end
position has however not yet been reached. All information is given in micro-steps.
This command can only be executed when remote control is active.

A Appendix – PC interface

C012 GOTO position in micrometers without reached

message
A012 Ready for next command Parameters: None.
The manipulator moves in all three axes at the specified speed to the specified position. If no

movement is required in one of the axes, then its speed is sent as 0. An acknowledgement is
sent as soon as the command has been fully evaluated and the next one can be sent. The end
position has however not yet been reached. All information is given in micrometers.
This command can only be executed when remote control is active.

C013 Wait until all motors stop Parameters: None.
A013 All motors stop Parameters: None.
The manipulator waits with its response until all motors stop, i.e. until the last final position has

been reached. If this command is sent when the motors have already stopped, the response is
sent immediately.

92

Parameters:
Px Py Pz Vx Vy Vz

A Appendix – PC interface

C014 Trigger short beep tones Parameters:

Number of beep tones
A014 All beep tones have been produced Parameters: None.
The number of short (100 ms) beep tones that has been set in the parameter is produced.

C015 Trigger long beep tones Parameters:

Number of beep tones
A015 All beep tones have been produced Parameters: None.
The number of long (1 second) beep tones that has been set in the parameter is produced.

C016 STORE memory position Parameters: Memory position
A016 Ready for next command Parameters: None.
The current position of the manipulator is stored under the memory position number stated in

the parameter. This storage is lost when the device is switched off or set to standby. Memory
positions 1, 2 and 3 correspond to the memory positions that can be accessed from the key­board.

C017 GOTO memory position Parameters: Memory position
A017 Ready for next command Parameters:

Px Py Pz end position
The manipulator moves in all three axes at the GOTO speed stored internally to the first position

stored at this memory position. Acknowledgement is sent as soon as all motors stop because
either the final position has been reached or because a limit switch has been activated. The sta­tus of the limit switches is included in the response. If positioning was successful the value is 0.

This command can only be executed when remote control is active.

A

PC interface

All command/reply names (e.g. C010) are composed of a string with no internal spaces.

93

A micrometer is equivalent to 25.6 micro-steps. One micro-step is equivalent to approx. 40
nanometers.

A

px py pz Position of the x/y/z axes in micro-steps "–640000" ... "+640000"
Px Py Pz Position of the x/y/z axes in micrometers "–25000" ... "+25000"
vx vy vz Speed of the x/y/z axes in micro-steps per second "0" ... "192000"
Vx Vy Vz Speed of the x/y/z axes in micrometers per second "0" ... "7500"
Version String with model/version e.g.: "5181 V1.00"
End

position

PC interface

Memory
position

A Appendix – PC interface

A3 List of parameters

Position of the limit switch in bit code "0" ... "64"

0 = all switches opened
1 = lower stop on X axis
2 = upper stop on X axis
4 = lower stop on Y axis
8 = upper stop on Y axis
16 = lower stop on Z axis
32 = upper stop on Z axis
64 = no position stored

Number under which a position is stored "1" ... "50"

A4 Error messages

E070 Wrong number of parameters
E071 Parameter 1 is not within the permissible range
E072 Parameter 2 is not within the permissible range
E073 Parameter 3 is not within the permissible range
E074 Parameter 4 is not within the permissible range
E075 Parameter 5 is not within the permissible range
E076 Parameter 6 is not within the permissible range
E077 The first character is not c or C
E078 The 2nd to 4th characters are not numbers
E079 The Opcode is unknown
E086 Remote control via the serial interface is not switched on
E087 The device is not in the routine state

94

A Appendix – PC interface

Note: Error messages for software 1.00 – 1.04

E060 The first character is not a c or C
E061 The 2nd to 4th characters are not digits
E062 The 5th character is not =
E064 The command is not known
E065 The command contains a wrong number of parameters
E066 A parameter has an inadmissible value
E070 The remote control is not operative

A

PC interface

95

Eppendorf Offices

SPAIN

SWITZERLAND

UNITED KINGDOM

USA

OTHER COUNTRIES

ASEAN

Eppendorf AG
Regional Office in Malaysia
Tel. [phone]
Fax [phone]

E-Mail: [e-mail]

Internet: www.eppendorf.com.my

AUSTRALIA / NEW ZEALAND

Eppendorf South Pacific Pty. Ltd.
Tel. [phone]
Fax [phone]
E-mail: [e-mail]
Internet: www.eppendorf.com.au

AUSTRIA

Eppendorf AG
c/o Schott Austria
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com

BRAZIL

Eppendorf do Brasil Ltda.
Tel. [phone]
Fax [phone]
E-Mail:
[e-mail]
Internet: www.eppendorf.com.br

CANADA

Eppendorf Canada Ltd.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com

CHINA

Eppendorf AG
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.cn

FRANCE

EPPENDORF FRANCE S.A.R.L.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.fr

GERMANY

Eppendorf Vertrieb
Deutschland GmbH
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.de

INDIA

Eppendorf India Limited
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.co.in

ITALY

Eppendorf s.r.l.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.it

JAPAN

Eppendorf Japan Co. Ltd.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.jp

NORDIC

Eppendorf Nordic Aps
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.dk

Eppendorf Ibérica S.L.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.es

Vaudaux-Eppendorf AG
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com

Eppendorf UK Limited
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.co.uk

Eppendorf North America
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorfna.com

see:
www.eppendorf.com/worldwide

In touch with life

Your local distributor: www.eppendorf.com/worldwide

Eppendorf AG · 22331 Hamburg · Germany · Tel. [phone] · Fax [phone]

E-Mail: [e-mail]

Eppendorf North America, Inc. · One Cantiague Road, P.O. Box 1019 · Westbury, N.Y. 11590-0207 USA

Tel. [phone] · Toll free phone [phone] · Fax [phone] · E-Mail: [e-mail]

Application Support

Europe, International: Tel. [phone] · E-Mail: [e-mail]

North America: Tel. [phone] ext. 2258 · E-Mail: [e-mail]

Asia, Pacific: Tel. [phone] · E-Mail: [e-mail]

eppendorf is a registered trademark.

B 5183 900.015-06/0807 · Printed in Germany