Eppendorf New Brunswick Excella E-24 Shaker User Manual

nualck™ Excella® E-24 Shaker

EN) manual

New Brunswick Shaker

Operating manual

™

Excella® E-24

The company reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.

Trademarks

New Brunswick and the New Brunswick Logo™ are trademarks of Eppendorf AG, Germany.

Excella

Trademarks are not marked in all cases with ™ or

is a registered trademark of Eppendorf, Inc., USA.

in this manual.

Eppendorf has attempted to identify the ownership of all trademarks from public records. Any omissions or errors are unintentional.

M1352-0051-A/022014

Table of contents

™

New Brunswick

Excella® E-24 Shaker

English (EN)

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2 Inspection of boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3.1 Operating modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3.2 Visible/audible user alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3.3 Data logging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3.4 Setpoint retention . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3.5 Automatic restart. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3.6 Drive interrupt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.2 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3.1 Personal injury and damage to device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.2 Unpacking the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.3 Utility requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.4 Installation of platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.5 Flask clamp installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

4.6 Electrical connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.1 Start/stop. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.2 Continuous (untimed) run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.3 Check setpoint. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.4 Timed functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.4.1 Setting the timer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.4.2 If the shaker is stopped: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.5 Alarm functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.5.1 Deactivating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.5.2 Reactivating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.6 Temperature setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.7 Temperature offset calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.8 Factory Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.9 Speed calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.9.1 Calibration adjustments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Table of contents

™

New Brunswick

Excella® E-24 Shaker

English (EN)

5.10 Power failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6 Operating controls and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

6.1 Control panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

6.1.1 User interface keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

6.1.2 Status indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.1.3 LED display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.1.4 Function indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

8 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

8.1 Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

8.2 Opening the service compartment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

8.2.1 Fuse replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

8.3 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

8.4 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

9 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

9.1 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

10 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.1 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.1.1 Device dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.1.2 Platform dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.1.3 Chamber dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.1.4 Required space/footprint. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.2 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.2.1 Speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.2.2 Capacity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

10.2.3 Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

10.3 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

11 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

11.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

12 Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Operating instructions

™

New Brunswick

Excella® E-24 Shaker

English (EN)

1 Operating instructions

1.1 Using this manual

 Carefully read this operating manual before using the device for the first time.

 Also observe the operating manual enclosed with the accessories.

 The operating manual should be considered as part of the product and stored in a location that is easily

accessible.

 When passing the device on to third parties, be sure to include this operating manual.

 If this manual is lost, please request another one. The current version can be found on our website http:/

/www.eppendorf.com.

1.2 Danger symbols and danger levels

1.2.1 Hazard symbols

Hazard point Material damage

Electric shock

1.2.2 Degrees of danger

The following degree levels are used in safety messages throughout this manual. Acquaint yourself with each item and the potential risk if you disregard the safety message.

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Symbols used

Example Meaning

 You are requested to perform an action.

• List.

Perform these actions in the sequence described.

References useful information.

Operating instructions

New Brunswick

English (EN)

™

Excella® E-24 Shaker

1.4 Abbreviations used

kWh

Kilowatt hours

Proportional/Integral

PPE

Personal Protective Equipment

RMA

Return Material Authorization

rpm

Revolutions Per Minute

UEL

Upper Explosion Limit

Volt Amps

VAC

Voltage in Alternating Current

2 Product description

2.1 Main illustration

Abb. 2-1: New Brunswick™ Excella® E-24 incubator shaker

New Brunswick

Product description

™

Excella® E-24 Shaker

English (EN)

Fig. 2-1: New Brunswick™ Excella® E-24 incubator shaker

1 Lid handle

2 User interface

3 On/off switch

Product description

New Brunswick

English (EN)

™

Excella® E-24 Shaker

2.2 Inspection of boxes

After you have received your order, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage to the carrier and to your local Eppendorf distributor immediately.

2.3 Features

The Excella E-24 Benchtop Incubator Shakers uses a unicentric counter-balanced drive mechanism. It provides horizontal plane rotary motion in a 1.91 cm (¾ in) diameter circular orbit. A Proportional/Integral (PI) microprocessor controls the speed and temperature over the entire range.

The E-24 operates from 7 °C above ambient to 60 °C. This range depends on relative humidity and other ambient factors. Ambient temperature is measured one meter from the front of the unit.

Erlenmeyer flasks, 2.8-liter Fernbach flasks, and a wide variety of tubes and plates can be accommodated using New Brunswick shaker accessories.

A wide variety of platforms can be used with the Excella E-24:

• Universal platforms are the most flexible, providing hole patterns for flask clamps, test tube racks and other accessories.

• Dedicated platforms are supplied with flask clamps attached; they are designed solely and expressly for this purpose.

• Test tube racks, microplate holders, and test tube rack holders are also available (a universal platform is needed for all test tube racks and holders).

For further information on these accessories (see Accessories on p. 35).

2.3.1 Operating modes

The E-24 may be operated in the following modes:

• Continuously: at a set speed and temperature, until user intervention.

• In a timed mode: run at a set speed, time and temperature for a period of up to 99.9 hours, after which the incubator shaker automatically shuts off, while the temperature is maintained at its setpoint.

2.3.2 Visible/audible user alarms

The E-24 is equipped with visual and audible alarms for the following conditions:

• The end of a timed run

• Deviations from speed setpoint (5 minutes after lid is closed)

• Deviations from temperature setpoint (5 minutes after lid is closed)

• Power failure

• Lid open

Product description

™

New Brunswick

Excella® E-24 Shaker

English (EN)

2.3.3 Data logging

RS-232 connection provided for data logging.

2.3.4 Setpoint retention

All setpoints and operating status are retained in non-volatile memory.

2.3.5 Automatic restart

Automatic restart after power is restored, indicated by flashing display.

2.3.6 Drive interrupt

Drive interrupt shuts off power to the incubator shaker when the lid opens. Acceleration/deceleration circuit prevents sudden starts and stops, minimizing both splashing and mechanical damage.

Product description

™

New Brunswick

Excella® E-24 Shaker

English (EN)

Safety

English (EN)

New Brunswick

™

Excella® E-24 Shaker

3 Safety

3.1 Intended use

The New Brunswick Excella E-24 is half incubator and half biological shaker, combining the best features of New Brunswick incubators and shakers into one versatile instrument. It provides gentle shaking and accurate control of temperature, enabling growth of hybridomas, insect cultures, mammalian and stem cells as well as both aerobic and anaerobic bacteria and yeast.

The New Brunswick Excella E-24 was designed for controlled incubation and shaking of biological samples. Do not use any heat-generating device inside the chamber.

CAUTION! Lack of safety due to incorrect accessories or spare parts

Accessories and spare parts that are not recommended by Eppendorf compromise the safety, function and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of non-recommended accessories and spare parts.

 Only use accessories and original spare parts recommended by Eppendorf.

3.2 Information on product liability

In the following cases, the designated protection of the device may be compromised.

The liability for the function of the device passes to the operator if:

• The device is not used in accordance with this operating manual.

• The device is used outside of the range of application described in the succeding chapters.

• The device is used with accessories or consumables that were not approved by Eppendorf.

• Service or maintenance is completed on the device by people who are not authorized by Eppendorf.

• The owner has made unauthorized modifications to the device.

3.3 Warnings for intended use

Before using the incubator shaker, read the operating manual and observe the following general safety instructions.

Safety

New Brunswick English (EN)

™

Excella® E-24 Shaker

3.3.1 Personal injury and damage to device

WARNING! Health risk due to poisonous, radioactive or aggressive chemicals

 Observe the national regulations for handling these substances as well as the material

safety data sheets and manufacturer's application notes.

 Wear personal protective equipment (PPE).

WARNING! Burns due to hot metal on the device and hot pistons

 Only touch the device and pistons when wearing protective gloves.

WARNING! Risk of crushing fingers with lid

 Do not reach between the lid and device, or into the lid locking mechanism, when opening

and closing the device.

 Always fully open the lid so it cannot fall and close.

CAUTION! Risk to health due to lifting heavy loads

 Only lift the device with another person or using a suitable aid.  Make sure to use a transport aid for transportation over long distances.

NOTICE! Damage due to overheating

 Do not place the device close to sources of heat (e.g., radiator, drying cabinet).  Do not expose the device to direct sunlight.  Ensure there is adequate distance to the wall and adjacent devices, on all sides of the

device, in order to guarantee unobstructed air circulation.

™

New Brunswick

Excella® E-24 Shaker

4 Installation

4.1 Selecting the location

NOTICE! Damage due to overheating

device, in order to guarantee unobstructed air circulation.

Select the location according to the following criteria:

• Suitable mains power connection according to the ID plate

• Stable, even and resonance-free base

• Well ventilated area and no direct sunlight to prevent additional temperature increases

• Ambient conditions of 10 °C to 35 °C, 20 % to 80 % non condensing

• Able to accommodate 200 lb

Installation

English (EN)

4.2 Unpacking the device

Keep the packing material and transport securing device for later transport or storage.

1. Remove the packing material.

2. Remove the transport securing device.

3. Use the details included in the scope of delivery to check that the delivery is complete.

4. Check all parts for damage in transit. Contact Eppendorf Service if parts are missing or transport damage is present.

4.3 Utility requirements

The following utility requirements are needed for operation:

Utility Requirement

Electricity • 230 V, 60 Hz, 1500 VA maximum

In all cases, voltage variations must not exceed ±10 %.

Installation

™

New Brunswick

Excella® E-24 Shaker

English (EN)

4.4 Installation of platform

A platform must be installed prior to use.

The shaker is shipped with 4 Allen head platform screws (circled in red, (see Fig. 4-1 on p. 14)) installed in the 4 bearing housing uprights. The screws must be removed before a platform can be installed.

Abb. 4-1: Platform screw locations

Fig. 4-1: Platform screw locations

1 Subplatform

1. Remove the 2 small plastic straps that secure the bearing housing in place for shipping.

2. Using the 7/32 in hex key provided, remove the 4 Allen head platform screws (circled in red, (see Fig. 4-1 on p. 14)) from the subplatform.

Save the screws for later use.

3. Place the selected platform on the subplatform. Align the mounting holes of the platform with the platform screw locations in the subplatform.

4. Insert the 4 Allen head platform screws removed in step 2. Tighten them with the 7/32 in hex key to secure the platform.

+ 30 hidden pages