Eppendorf New Brunswick -86 °C Freezers Premium User Manual

Operating manual

Draft

—

Premium -86 C Freezers

see

on p.Fig.Tab.p

.

Op

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erating manua

August 1, 2012

New Brunswick -86 °C Freezers

Premium

Operating manual

M1288-0053

Revision U

Copyright

Copyright© 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of
the copyright owner.

The company reserves the right to change information in this document without notice. Updates to information in this
document reflect our commitment to continuing product development and improvement.

Trademarks

Eppendorf® is a registered trademark of Eppendorf AG, Germany.

New Brunswick and the New Brunswick Logo™ are trademarks of Eppendorf AG, Germany.

BioCommand

®

is a registered trademark of Eppendorf AG, Germany.

Innova® is a registered trademark of Eppendorf AG, Germany.

S.M.A.R.T. Plus™ is a pending trademark of Eppendorf AG, Germany.

Trademarks are not marked in all cases with ™ or

®

in this manual.

Eppendorf has attempted to identify the ownership of all trademarks from public records. Any omissions or errors are
unintentional.

August 29, 2012
Revision U
M1288-0053

Premium -86 °C Freezers — Operating manual

Table of contents

1 Table of contents

1

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2.1 Manual conventions used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2.2 Health and safety at work act 1974. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

3 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3.2.1 Inspection of boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3.2.2 Packing list verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3.3 Product versions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.4 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.3 Mains/Power plugs and Receptacles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.4 Installing the shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.5 Lockable freezer handle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Table of contents

5 Operating controls and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5.1 Controls and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.1 Getting started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.1.1 Plug in . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.1.2 Turning the freezer On/Off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.1.3 Alarm/battery activation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6.1.4 Testing the alarm monitoring socket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6.1.5 Vacuum effect . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.2 Programming the freezer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.2.1 Setting operating temperature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.2.2 Setting high alarm setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.2.3 Setting low alarm setpoint. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

6.2.4 Checking temperature and alarm setpoint settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

6.2.5 Setting the alarm delay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.2.6 Changing lock codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.2.7 Setting the temperature offset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.3 Battery backup switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.4 Alarm monitoring socket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

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Table of contents

Premium -86 °C Freezers — Operating manual

7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

7.1.1 Painted surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

7.1.2 Panels and shelves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

7.1.3 Air intake grille and filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

7.1.4 Heated vent port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

7.1.5 Door or lid seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.2 Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.2.1 Lubrication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.2.2 Defrosting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.2.3 Removing the inner doors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.2.4 Replacing the inner door. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.2.5 Electrical components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

8 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8.3 After a mains/power failure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

8.4 Interior warming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

9.1 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

9.1.1 Upright freezers specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

9.1.2 Chest freezers specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

10 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1.1 TCA-3 independent temperature monitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1.2 Auto-dialers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1.3 Temperature probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1.4 Validation packages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1.5 External voltage stabilizers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1.6 Padlock adapter kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1.7 CO

10.1.8 Inventory racking systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1.9 Chart recorder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1.10 New Brunswick BioCommand SFI datalogging software (RS-485 interface) . . . . . . . . . . . . . . . . . . 38

11 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

11.1 Shut down. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

11.2 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

11.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

12 Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

and LN2 back-up systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

2

4

Premium -86 °C Freezers — Operating manual

1 Operating instructions

1 Operating instructions

1.1 Using this manual



Carefully read this operating manual before using the device for the first time.

 Also observe the operating manual enclosed with the accessories.

 The operating manual should be considered as part of the product and stored in a location

that is easily accessible.

 When passing the device on to third parties, be sure to include this operating manual.

 If this manual is lost, please request another one. The current version can be found on our

website http://www.nbsc.com

1.2 Danger symbols and danger levels

1.2.1 Hazard symbols

Hazard point Burns

Electric shock Material damage

.

1

Operating instructions

Crush

1.2.2 Degrees of danger

The following degree levels are used in safety messages throughout this manual. Acquaint
yourself with each item and the potential risk if you disregard the safety message.

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Symbols used

Example Meaning

 You are requested to perform an action.

1.

2.

Perform these actions in the sequence described.

• List.

References useful information.

5

1

Operating instructions

Premium -86 °C Freezers — Operating manual

1.4 Abbreviations used

A Amp

CFC Chlorofluorocarbons

°C Degree Celsius

HCFC Hydrochlorofluorocarbon

HFC Hydrofluorocarbon

Hz Hertz

kg Kilogram

lb Pound

m Meter

min Minute

mm Millimeter

N/A Not applicable

rpm Revolutions per Minute (min

ULT Ultra-Low Temperature

V Volt

-1

)

6

Premium -86 °C Freezers — Operating manual

2 Safety

2Safety

2.1 Intended use

New Brunswick Premium freezers are designed to provide precise, ultra-low temperature
environments for cold storage of scientific or medical materials. They are designed to provide
ultra-low temperature sample storage from -50 °C to -86 °C at 32 °C maximum ambient
operating temperature.

2.2 Warnings for intended use

2.2.1 Manual conventions used

Risk of personal injury

 BEFORE connecting the freezer to the mains/electrical supply, make sure that the mains/

WARNING!

power supply matches the requirements of the equipment. Check the specification plate
(located on the side of the freezer) for the electrical requirements. The equipment should be
connected to an earthed/grounded outlet socket.

Risk of personal injury

 Flammable warning messages alert you to possible risks of of personal injury and equipment

WARNING!

damage: protect the system from sparks and flames.

Risk of personal injury

 Do not use this equiment in a hazardous atmosphere or with hazardous materials for which

CAUTION!

the equipment was not designed.

 Please read the entire operating manual before attempting to use this equipment. If

operational guidelines are not followed, personal injury may occur.

Risk of personal injury

 Crush Warning messages alert you to specific procedures or practices regarding heavy

CAUTION!

objects which, if not followed correctly, could result in serious personal injury.

2

Safety

Risk of material damage

 This equipment must be operated as described in this manual.

NOTICE!

2.2.2 Health and safety at work act 1974

 Please read the entire operating manual before attempting to use this equipment. If

operational guidelines are not followed, equipment damage may occur.

(FOR THE UNITED KINGDOM)

New Brunswick Scientific, as manufacturers and suppliers of laboratory equipment, are obliged
under the terms of the above Act to provide our users with instructions on the safe installation,
operation and maintenance of our equipment.

Our equipment is designed to acceptable standards and does not entail any hazard if used, as
advised in the attached instructions.

The following safety precautions should be observed by all personnel using this equipment:

1. Read and understand this manual. If in doubt, contact your local New Brunswick or
Eppendorf sales office.

2. Do not remove any covers. There are no operable controls other than those referred to in this
manual. There are voltages in excess of 41.5 volts AC behind the covers.

7

2

Premium -86 °C Freezers — Operating manual

3. Use freezer gloves at all times when loading or unloading the equipment. The temperature of
operation is such that direct contact with the cold contents or inside the equipment can burn
unprotected skin.

4. Observe good housekeeping practices, at all times keeping the equipment and the adjacent
areas clean, dry and uncluttered.

5. Should any malfunctions occur or be suspected, immediately call a qualified service engineer
to investigate.

Safety

8

Premium -86 °C Freezers — Operating manual

3 Product description

3 Product description

3.1 Main illustration

Abb. 1: Upright freezer - Side an d front views

12 3

3

Product description

4

5

76891011

Fig. 1: Upright freezer - Side and front views

1 Heated vent port 2 Door handle (lockable)

3 Control panel/display 4 Model label

5 Battery switch behind lockable panel 6 On/Off circuit breaker behind lockable panel

7 Transport castors 8 Chart recorder (optional)

9 Air filter grille 10 Quarter turn fastener

11 Specification plate

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Premium -86 °C Freezers — Operating manual

3

Abb. 2: Chest freezer - Front view

23

1

4
5
6

7

8

9

Fig. 2: Chest freezer - Front view

1 Heated vent port (inside compressor housing) 2 Lockable handle

3 Control panel/display 4 Battery switch (alarm) behind lockable panel

5 On/Off circuit breaker behind lockable panel 6 Chart recorder (optional)

Product description

7 Quarter turn fasteners 8 Air filter grille

9 Braked castors (front)

3.2 Delivery package

3.2.1 Inspection of boxes

Inspect the boxes carefully for any damage that may have occurred during shipping. Report any
damage to the carrier and to your local New Brunswick Sales Order Department immediately.

3.2.2 Packing list verification

Unpack your order, saving the packing materials for possible future use. Save the operating
manual for instruction and reference. Verify against your New Brunswick packing list that you
have received the correct materials, and that nothing is missing. If any part of your order was
damaged during shipping, is missing, or fails to operate, fill out the "Customer Feedback" form,
available online at www.nbsc.com/CustomerFeedback.aspx

Risk of material damage

 Inspect the cabinet panels for punctures or other damage that compromises the integrity of

NOTICE!

the product.

 Any unauthorized punctures or other damage deliberately made to the cabinet walls will

invalidate the warranty.

.

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Premium -86 °C Freezers — Operating manual

3.3 Product versions

3.3.1 Introduction

This manual provides the user with the necessary information for installation and operation of
New Brunswick's Premium ultra-low temperature freezers. It also provides some preliminary user
maintenance information.

This manual covers all versions of the Premium freezer models:

Model (230 V, 50 Hz) Capacity

U410 410 liters (14.5 cubic feet)

U570 570 liters (20.0 cubic feet)

U700 700 liters (24.6 cubic feet)

C340 340 liters (12.0 cubic feet)

C660 660 liters (23.3 cubic feet)

3.4 Features

Model (208 - 230 V, 60 Hz) Capacity

U410 410 liters (14.5 cubic feet)

U570 570 liters (20.0 cubic feet)

U700 700 liters (24.6 cubic feet)

C660 660 liters (23.3 cubic feet)

Model (120 V, 60 Hz) Capacity

U410 410 liters (14.5 cubic feet)

U570 570 liters (20.0 cubic feet)

U700 700 liters (24.6 cubic feet)

C340 340 liters (12.0 cubic feet)

Premium freezers are totally free of CFCs (Chlorofluorocarbons) and HCFCs
(Hydrochlorofluorocarbons). They use HFCs (Hydrofluorocarbons) as refrigerants.

The freezers are manufactured using high quality steel and electronics for long life. The Premium
freezers have many features designed to provide ease of use and maintanence, security and
reliability to your ultra-low temperature storage needs. They are built to stringent regulatory
requirements for safety and environmental friendliness and disposability, and they are CE and UL
certified.

Features include:

• Designed to be exceptionally energy efficient and eco-friendly.

• A setpoint keypad and digital temperature readout are provided on a control panel, located on

the door of the upright freezers and on the console on the right side of chest freezers.
Controls for setting the freezer temperature and alarm warning setpoints are enabled by an
electronic lock. A unique code, which is selected by the user through the keypad, sets this
lock. The code can be changed at any time, provided the current setting of the lock is known.

On delivery, the lock code is "0000" (unlocked).

• Indicator lamps on the control panel provide warnings of power loss, system failure,

exceeding High/Low temperature setpoints, low battery voltage, and filter blockage. There is
also an LED to indicate when the freezer is under remote control via the RS-485 optional
computer interface.

• Freezers are totally free of CFCs (Chlorofluorocarbons) and HCFCs

(Hydrochlorofluorocarbons). They use industrial grade, commercially available HFCs
(Hydrofluorocarbons) as refrigerants.

• Heavy-duty commercially available compressors provide rapid temperature pull-down and

recovery after door opening.

3

Product description

11

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Premium -86 °C Freezers — Operating manual

• A heated port with ice-clearing plunger prevents vacuum formation enabling the outer door to

be easily opened at anytime.

• All interior panels and shelves are made of high grade corrosion-resistant stainless steel,

making them durable and easy to keep clean and sterilize.

• Heavy duty castors enable easy installation and relocation.

• Two access ports allow easy addition of sensors, or of back-up systems that can provide

cooling protection for your samples in the case of a power outage or other system failure.

• An alarm contact is provided for connection to an external monitoring device or system.

• The freezers can be fitted with an optional 7-day circular chart recorder to provide

independent temperature recording.

• An automatic reset restarts freezers at random 15 second intervals to protect the

microprocessor controller from damage by electrical spikes caused by multiple freezers
turning on at once.

• Lockable freezer handles on outer door provide added security against unauthorized users.

• Multiple accessories are offered, including CO

systems, external voltage stabilizer, inventory racking, and more.

/LN2 back-up systems, remote monitoring

2

Product description

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Premium -86 °C Freezers — Operating manual

4 Installation

4 Installation

4.1 Preparing installation

Risk of personal injury

 DO NOT attempt to lift any freezer by hand. Preferred lifting for loading and unloading is by

WARNING!

NOTICE!

4.2 Selecting the location

mechanical lifting equipment.

Risk of material damage

 Maintenance, adjustment and repair work should be carried out only by QUALIFIED,

EXPERIENCED personnel who have been AUTHORIZED to undertake such work by
Eppendorf or its authorized agents.

 Failure to use authorized service agents will invalidate the warranty.

All freezers are mounted on castors for ease of movement. Upright freezers and chest freezers
have locking front castors to stop the freezer from rolling once it is in place; locking front castors
do not provide leveling adjustment, so the site chosen for the freezer must have a flat, level floor.

Position the freezer to allow disconnection to be made in respect to removal of the plug or
appliance coupler, also the free air entry through the intake grille in the front and free air exit from
the back. Provide a clearance of at least 150 mm (6 in) on all sides.

For efficient temperature control, the freezer should be placed in a shaded area, away from
sources of excessive heat. For maximum cooling capability, the product should be located in an
air-conditioned room.

4

Installation

4.3 Mains/Power plugs and Receptacles

New Brunswick Freezers are offered with a variety of mains/power cords to accommodate local
voltage requirements. To determine the mains/power receptacle required in your lab, first identify
the plug we provide from the list below and check the freezer rating plate for mains/power
requirements, then see the table which follows.

• All 120 V, 60 Hz freezers use plug B, with the exception of Innova U101 freezers which use

plug A

• All 208 - 230 V, 60 Hz freezers use plug C

• All 230 V, 50 Hz freezers are provided with both plugs D and E

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Premium -86 °C Freezers — Operating manual

4.4 Installing the shelves

Model U410, U570, and U700 freezers are fitted with four adjustable shelves. These can be
positioned in 12.7 mm (½ in) steps anywhere throughout the freezer.

To effectively utilize racks within the freezer, be sure to position them so that each shelf is aligned
with the bottom of each inner door.

Perform the following steps to install the shelves:

1. Ensure that the freezer is turned off and unplugged.

2. Remove the protective plastic coating from the shelf.

3. Position the four shelf clips evenly within the freezer by squeezing the clip, then inserting it
into the shelf support within the freezer.

4. Place the shelf in the freezer, making sure all four shelf clips are supporting the weight of the
shelf.

To readjust the shelf or shelf clips, gently squeeze the shelf clip to release it from the side of the
freezer, then reposition it as needed.

4.5 Lockable freezer handle

Freezers are supplied with lockable handles.

The C340 and C660 are fitted with a quarter turn key lock.

The upright freezer handle is fitted with barrel locks (push in and turn key to lock, turn key to
unlock; the barrel will only lock when a key is turned to the lock position). The barrel lock may be
removed from the upright freezer handle if the lock feature is not required.

An optional padlock adaptor can provide extra security by allowing the addition of a
customer-supplied padlock to secure the freezer handle.

Installation

Removing the upright freezer handle barrel lock

Perform the following steps to remove the barrel lock from the upright freezer handle, if the lock
feature is not required:

1. Open the freezer door and place the freezer handle in closed position.

2. Remove the two screws from behind the lock barrel.

1

2

3

4

1 Freezer handle 2 Screw (1 of 2)

3 Lining plate 4 Freezer door wall

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Premium -86 °C Freezers — Operating manual

3. Remove the lining plate and lock barrel.

4. Insert the plastic blanking plug supplied.

5. Insert the lining plate and screw in the two screws.

It is important that the handle lock lining plate be installed at all times.

Hint!

Risk of material damage

 DO NOT SLAM THE DOOR WITH THE HANDLE IN THE CLOSED POSITION.

NOTICE!

6. Place freezer handle in open position and close freezer door.

The door handle has a cam action to pull the door closed and a reverse cam action to break the
seal so the door can be opened. When closing the outer door, ensure that the cam is engaged for
correct operation. The initial vacuum inside the cabinet may cause the door to appear closed, but
when the vacuum releases, the door will open. Always ensure the handle is properly engaged. It
is important that the heated vent port is kept clear. This will avoid putting undue stress on the
handle mechanism.

4

Installation

15

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Premium -86 °C Freezers — Operating manual

5 Operating controls and function

5 Operating controls and function

5.1 Controls and function

Operating controls are located on a control panel mounted in the door of the upright freezers, and
on the console on the right side of chest freezers.

Every New Brunswick freezer is equipped with S.M.A.R.T. Plus™ diagnostic software, to help
identify the cause of a fault or setpoint variance.

Abb. 3: Display panel & keypad

This section describes the controls and function of the control panel, (see Fig. 3 on p. 16).

1315

1

TEMPERATURE

23456

Operating controls and function

Fig. 3: Display panel & keypad

12

1

REMOTE-CONTROL

8

TEMP-ALARM

FILTER-CLEAN BATTERY-LOW

POWER-FAIL FAULT

C

78 90EC

ALARM

SET

TEMP

LOW

ALARM

HIGH

ALARM

LOCK

CODE

CHANGE

TEST/
MUTE

14

11

10927543 6

Temperature °C Display

Item Name Function

1 TEMPERATURE °CThe digital display normally shows the current internal

temperature of the freezer. Temperature is displayed in 1°C
increments.

High alarm/low alarm

Item Name Function

2 & 8 [HIGH/LOW]

TEMP-ALARM

The TEMP-ALARM illuminates and the audible alarm sounds if
the freezer’s internal temperature is above/below the
user-selected alarm setpoints. The LED illuminates as soon as
the setpoint is passed. The high temperature audible alarm has
a programmable delay (see Setting the alarm delay on p. 24).
After the temperature returns to the normal range, the
TEMP-ALARM switches off and the audible warning stops.

16

The audible alarm can be silenced by pressing the ALARM TEST/MUTE KEY. If the temperature

Hint!

has not returned to normal range after the programmed time period, the audible warning will
sound again. This pattern will continue to repeat until the temperature returns to normal.

Premium -86 °C Freezers — Operating manual

Temp-alarm light

Item Name Function

8 TEMP-ALARM Should a mains/power failure cause the temperature to surpass

the alarm setpoint, the TEMP-ALARM illuminates. (The audible
alarm will already be sounding due to the mains/power failure).
The TEMP-ALARM lamp will extinguish when the temperature
returns to normal set range. Cancel the TEMP-ALARM by
pressing the ALARM TEST/MUTE KEY.

Power fail light

Item Name Function

3 POWER-FAIL Illuminates if the mains/power supply fails, flashing at

approximately 10-second intervals, accompanied by an audible
alarm. When mains/power is restored, the indicator goes off
and the audible alarm stops. (The battery must be switched on
and charged for this indicator to operate.)

Fault light

Item Name Function

4 FAU LT Illuminates if there is a system failure within the freezer.

Interfacing with the S.M.A.R.T. Plus™ diagnostics via the
control panel, the fault can be determined (see Error messages
on p. 32). System failure is accompanied by an audible alarm.
Correction of the fault extinguishes the light and audible alarm.

5

Operating controls and function

Battery low light

Item Name Function

5 BATTERY-LOW With mains/power ON: illuminates if battery voltage is below 6

volts, flashes when voltage drops to 5 Volts. With mains/power
OFF: if battery voltage drop below 5.5 Volts, this fault indicator
will stop functioning.

Filter clean light

Item Name Function

6 FILTER-CLEAN Illuminates, accompanied by an audible alarm, to indicate a

blocked or dirty filter. Filter is located on the front at the bottom
of all freezers. Remove by turning the two thumbscrews on the
filter holder ¼ turn. Clean filter by washing in mildly soapy
water, then air dry. If filter warning light does not go out after
replacing the cleaned filter, contact your local New Brunswick or
Eppendorf service representative.

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5

Operating controls and function

Remote control light

Item Name Function

12 REMOTE

CONTROL

The keypad controls are locked when the freezer is under remote control.

Hint!

Set temp key

Operation in normal mode with LOCK lamp off.

Item Name Function

7 SET TEMP Displays current temperature setting. Used to change

High alarm/low alarm keys

Operation in normal mode with LOCK lamp off.

Indicates when freezer is operating under remote computer
control via the optional RS-485 interface port and New

Brunswick's BioCommand
data logging software.

temperature settings.

®

SFI software, or other laboratory

Item Name Function

2 HIGH-ALARM Displays current high alarm temperature setting.

2 LOW-ALARM Displays current low alarm temperature setting.

Lock key

Operation in normal mode with LOCK lamp off.

Item Name Function

9 LOCK Locks and unlocks the control panel for programming

sequence.

Code change key

Operation in normal mode with LOCK lamp off.

Item Name Function

10 CODE CHANGE Used to change freezer lock codes. Inactive in normal mode.

Alarm test/mute key

Operation in normal mode with LOCK lamp off.

Item Name Function

11 ALARM TEST/

MUTE

Sounds the audible alarm. If the audible alarm is on due to a
fault condition, press this key to silence the alarm. The lamp
LED lights can also be tested by pressing this key. The lights
should all illuminate and the display should read “8888”.

18

Using the TEST/MUTE button does NOT cancel remote alarm monitor socket switching.

Hint!

Premium -86 °C Freezers — Operating manual

"E" key

Operation in normal mode with LOCK lamp off.

Item Name Function

13 E Used to enter data when programming.

"C" key

Operation in normal mode with LOCK lamp off.

Item Name Function

14 C Used to cancel data when programming.

Numerical keys

Operation in normal mode with LOCK lamp off.

Item Name Function

15 NUMERICAL

KEYS (1-0)

Used to input data when programming. Keys 8 and 9 are also
used to program alarm delays (see Setting the alarm delay on
p. 24).

5

Operating controls and function

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Premium -86 °C Freezers — Operating manual

6 Operation

6 Operation

6.1 Getting started

6

Operation

Risk of personal injury

 BEFORE connecting the freezer to the mains/electrical supply, make sure that the mains/

WARNING!

6.1.1 Plug in

power supply matches the requirements of the equipment. Check the specification plate
(located on the side of the freezer) for the electrical requirements. The equipment should be
connected to an earthed/grounded outlet socket.

Once you have verified that the mains/power supply matches the electrical requirements of the
freezer, connect the product to the mains/power supply using the mains/power cord provided.

Risk of personal injury

 If the freezer’s voltage rating does not match your mains/electrical supply, or if the plug on the

WARNING!

mains/power cord does not fit the outlet, do not plug the freezer in.

 Contact your laboratory supervisor, safety officer, or qualified service or electrical engineer.

Risk of material damage

 Some freezers are supplied with more than one removable mains/power cord. Utilize the cord

NOTICE!

6.1.2 Turning the freezer On/Off

that matches your power receptacle. Check the voltage rating plate on the side of the freezer,
to confirm that the freezer is compatible with your laboratory mains/power supply.

The ON/OFF circuit breaker is located within the lockable panel at the bottom right-hand corner
of the upright freezer or to the left of the control panel on the chest model.

To remove the lockable panel and turn the circuit breaker and battery switch On/Off:

1. Insert and turn the key (provided) one quarter turn to the right.

The key can be removed to prevent access.

Hint!

2. Remove the panel.

3. Set the ON/OFF circuit breaker and battery switch to the I (ON) position.

The temperature display illuminates immediately.

Abb. 4: Upright freezers switch location

Fig. 4: Upright freezers switch location

1 Battery switch 2 On/Off circuit breaker switch

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Abb. 5: Chest freezers switch location

Fig. 5: Chest freezers switch location

1 Battery switch 2 On/Off circuit breaker switch

The compressors will not operate for approximately three minutes after connection of the mains/

Hint!

6.1.3 Alarm/battery activation

power supply, because there is an automatic delay device in the circuit. Temperature and alarm
settings can be adjusted immediately.

The equipment is delivered with the battery deactivated. The Power Fail alarm is activated by the
battery rocker switch within the lockable panel, which is located at the bottom right-hand corner.
The switch is labelled I (ON) and O (OFF) (see Fig. 4 on p. 20) and (Fig. 5 on p. 21).

 To activate the alarm, place the battery switch in the I position.

1

6

2

Operation

Failure to turn on the battery switch may lead to a discharged battery, low battery alarm

Hint!

indication, and/or a disabled alarm system.

 After activating the alarm, test its operation by pressing the ALARM TEST/MUTE key on the

display.

The audible alarm should sound.

The ALARM TEST/MUTE key also tests the LED lamps. All of the LEDs should light up together
when the button is pressed.

Pull down time to -86 °C will be dependent on the freezer size and model (see Specifications on

Hint!

p. 34). The alarm will sound every 30 minutes until the temperature setpoint is reached. Use the
ALARM TEST/MUTE key to mute the alarm during this initial pull-down period.

If the freezer is turned off during the initial pull-down period, the alarm will activate 30 minutes
after switching it back on.

The factory-set temperature is -80°C.

6.1.4 Testing the alarm monitoring socket

The freezer is fitted with a remote alarm socket for testing power-fail and low battery alarms, and
for connection to an external building monitoring system or optional auto-dialer, (see Alarm
monitoring socket on p. 26). To test the alarm monitoring socket.

 Tur n o f f (O) the ON/OFF circuit breaker.

This will test the POWER FAIL and ALARM output at the same time.

The battery must be switched on to test the POWER FAIL. The remote alarm facility provides
voltage-free contacts rated at 1 amp, 24 volts maximum.

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6.1.5 Vacuum effect

After closing the freezer door following access to the contents, a vacuum may be created. Before
the door can be opened again, it may be necessary to wait two or three minutes for the vacuum
to be released by the vent port. Do not try to force the door. During the release of the vacuum,
a slight hissing may be heard. In order to minimize vacuum formation, the vent heater assembly
has a spring-loaded plunger to clear ice from the inside of the vent.

Be careful not to place a rack directly against the vent, as this will inhibit the plunger’s ability to

Hint!

6.2 Programming the freezer

operate correctly (see Heated vent port on p. 28).

Operation

Set the freezer to any temperature within the range from -50 °C to -86 °C.

All temperature setpoints are automatically negative °C.

Hint!

6.2.1 Setting operating temperature

To set the operating temperature for the freezer:

1. Press the LOCK key.

The LOCK lamp will flash if a lock code (password protection) is required, (see Changing lock

Hint!

codes on p. 24).

The LOCK lamp will illuminate, indicating the system is unlocked and parameters can be
changed.

2. Press the SET TEMP key.

Its indicator will flash and the display will indicate 0.

3. Using the numerical keys, enter a new temperature (from -50 °C to -86 °C).

The temperature selected will appear in the TEMPERATURE display.

4. When the correct temperature is displayed, press the E key to enter the data. To set the high
alarm setpoint skip to (see Setting high alarm setpoint on p. 22), step 2.

The SET TEMP lamp will go off.

5. Press the LOCK key to exit programming.

The LOCK lamp will go off and the freezer will return to normal mode.

Press the C key to clear the display during programming.

Hint!

6.2.2 Setting high alarm setpoint

The high alarm setpoint may not be warmer than -10 °C and may not be less than within +5° of
the operating temperature. The default setting is +5° from the temperature setpoint.

1. Press the LOCK key.

The LOCK lamp will flash if a lock code (password protection) is required, (see Changing lock

Hint!

codes on p. 24).

2. Press the HIGH ALARM key.

22

The LOCK lamp will illuminate, indicating the system is unlocked and parameters can be
changed.

Its indicator will flash and the display will indicate 0.

Premium -86 °C Freezers — Operating manual

3. Using the numerical keys, enter a new alarm setpoint temperature.

The selected temperature will appear in the TEMPERATURE display.

4. When the correct temperature is displayed, press the E (Enter) key to enter the data. To set
the low alarm setpoint skip to (see Setting low alarm setpoint on p. 23), step 2.

The HIGH ALARM indicator will turn off.

5. Press the LOCK key to exit programming.

The LOCK lamp will go off and the freezer will return to normal mode.

Press the C key to clear the display during programming.

Hint!

6.2.3 Setting low alarm setpoint

The low alarm setpoint may not be colder than -91 °C and may not be more than within -5° of the
operating temperature. The default setting is -5 °C from the temperature setpoint.

1. Press the LOCK key.

The LOCK lamp will flash if a lock code (password protection) is required, (see Changing lock

Hint!

codes on p. 24).

6

Operation

The LOCK lamp will illuminate, indicating the system is unlocked and parameters can be
changed.

2. Press the LOW ALARM key.

Its indicator will flash and the display will indicate 0.

3. Using the numerical keys, enter a new alarm setpoint temperature.

The selected temperature will appear in the TEMPERATURE display.

4. When the correct temperature is displayed, press the E (Enter) key to enter the data.

The LOW ALARM indicator will turn off.

5. Press the LOCK key to exit programming.

The LOCK lamp will go off and the freezer will return to normal mode.

Press the C key to clear the display during programming.

Hint!

6.2.4 Checking temperature and alarm setpoint settings

To view the currently set operating temperature, high alarm setpoint, or low alarm setpoint for the
freezer.

 Press the SET TEMP key, HIGH ALARM key, or the LOW ALARM key and read the display.

If you press the SET TEMP, HIGH ALARM or LOW ALARM key while the LOCK key lamp is

Hint!

flashing, the display will read ---- , which indicates that the freezer is locked.

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6

Operation

Premium -86 °C Freezers — Operating manual

6.2.5 Setting the alarm delay

The HIGH ALARM audible alarm and the REMOTE ALARM monitoring socket can be
programmed to a time delay set between 0 and 40 minutes.

The default time delay is 30 minutes. If the time delay is set to 0 minutes, the system will program
it as 15 seconds.

Press KEY 8 to display the High Temperature audible alarm delay, and press KEY 9 to display

Hint!

the Remote Alarm Socket switching delay.

To set the audible HIGH ALARM delay (KEY 8):

1. Press the LOCK key.

The LOCK lamp illuminates, indicating the system is unlocked and parameters can be
changed.

2. Press keypad button 8.

pp flashes on the display.

3. Enter the desired value (e.g., press keypad buttons 1 and 0 to designate 10 minutes).

4. Press the E (Enter) key.

The LOCK lamp goes out.

To set the REMOTE ALARM socket time delay (KEY 9):

1. Press the LOCK key.

The LOCK lamp illuminate, indicating the system is unlocked and parameters can be
changed.

2. Press keypad button 9.

pp flashes on the display.

3. Enter the desired value (e.g., press keypad button 5 to designate 5 minutes).

4. Press the E (Enter) key.

The LOCK lamp goes out.

If the number entered is valid, --- flashes on the display, the value has been stored, and the
LOCK lamp goes out. (This is a one-shot operation.)

If the number entered is out of range, –EE– shows on the display and the operation will need to
be repeated using a valid number.

6.2.6 Changing lock codes

If you enter a lock code when there is none, or if you replace an existing lock code with a new

Hint!

one, take note of the new code before you enter it.

If the code is forgotten, you will need to contact Customer Service to regain access to the
programming mode of the freezer.

The freezer is delivered unlocked. To change the code, the freezer must be unlocked. If a lock
code has already been set (indicated by the LOCK lamp flashing when the LOCK key is
pressed), that same code must be entered to unlock the freezer. When the freezer is unlocked,
the LOCK lamp is on (not flashing).

Once the freezer is unlocked, follow these steps to set a new lock code:

24

1. Press the CODE CHANGE key.

The lamp will flash and the display will go blank.

2. Using the numerical keys, enter the new four-digit number. Check it on the display.

3. Press the C key to cancel the entry if the display shows it to be incorrect, then enter the
correct number.

Premium -86 °C Freezers — Operating manual

4. When the number is correct, record the new number somewhere secure. Then press the E
(Enter) key.

The CODE CHANGE indicator will turn off.

5. Press the LOCK key.

Its indicator lamp will turn off.

The freezer now has a new lock code. If at any time you wish to change this code, you will have
to enter this code to unlock the system before you can enter a new code.

Setting the lock code to 0000 disables the lock completely. With the 0000 code, you would need
only press the LOCK key to reprogram the freezer.

6.2.7 Setting the temperature offset

The temperature offset function enables to add a temperature offset to the factory defined
temperature settings.

1. Press the LOCK Key.

2. Press the C key to access the offset function.

3. Press 0, 1, 2, 3, or 4 key to set the offset in degrees.

4. Press the ENTER key to confirm selection.

Set temperature offset to "0" for no offset.

Hint!

6

Operation

6.3 Battery backup switch

This is a rocker switch labeled I/O behind the locked front panel. In the O position, the battery is
disconnected. This position should only be used while in transit, in storage, or to change the
battery.

At all other times the switch should be kept in the I position for the battery to be charged, and for
the alarm function to be available in the event of mains/power failure. (Failure to set the switch
may result in impaired battery life, and the alarm will not trigger if the mains/power fails.)

With the battery switch on, during a mains/power failure, the internal freezer temperature will be
displayed at ten-second intervals, and the audio alarm will also sound. The audible alarm may be
muted by pressing the ALARM TEST/MUTE key on the control panel, but will sound again after
30 minutes if the fault has not been corrected. Pressing the same button again will mute the
alarm for an additional 30 minutes; the pattern will continue to repeat until the initial problem is
corrected.

25

1

2

3

123

6

Operation

Premium -86 °C Freezers — Operating manual

6.4 Alarm monitoring socket

The freezers are provided with an alarm monitoring socket at the rear of the freezer and a
matching plug for external monitoring. This plug can be connected either to a central monitoring
system such as a building management system, or to a remote alarm via an auto-dialer.

Abb. 6: Upright freezers alarm monitoring socket

Fig. 6: Upright freezers alarm monitoring socket

1 RS-485 connector (optional) 2 Alarm monitoring socket

3 Mains/power socket

Abb. 7: Chest freezers alarm mo nitoring socket

Fig. 7: Chest freezers alarm monitoring socket

1 RS-485 connector (optional) 2 Alarm monitoring socket

3 Mains/power socket

NOTICE!

26

The configuration of the socket is shown in (Fig. 8 on p. 27) and (Fig. 9 on p. 27), as viewed from
the rear of the freezer. Within the freezer, the socket is connected to voltage-free contacts rated
at 24 volts, 1 amp. In normal operation, with the mains/power on, pin 1 is connected to pin 2 (N/
C), and in the alarm condition, with mains/power off, pin 1 is connected to pin 3.

The High Temperature Alarm output to the Remote Alarm Monitoring Socket can be programmed
to a set time delay (see Setting the alarm delay on p. 24).

Risk of material damage

 Hazardous voltages must not be connected to the remote alarm socket. Max Rating 24 V 1 A.

Premium -86 °C Freezers — Operating manual

Abb. 8: Remote alarm socket - upright f reezer

Fig. 8: Remote alarm socket - upright freezer

Abb. 9: Remote alarm socket - chest free zer

6

Operation

Fig. 9: Remote alarm socket - chest freezer

27

7

Premium -86 °C Freezers — Operating manual

7 Maintenance

7 Maintenance

7.1 Cleaning

Risk of material damage

 Maintenance, adjustment and repair work should be carried out only by QUALIFIED,

NOTICE!

7.1.1 Painted surfaces

7.1.2 Panels and shelves

EXPERIENCED personnel who have been AUTHORIZED to undertake such work by
Eppendorf or its authorized agents.

 Failure to use authorized service agents will invalidate the warranty.

All exterior paint work and inner doors should be cleaned using a solution of mild detergent in
water. Do not use abrasive cleaners or solvents.

The interior panels and shelves are made of stainless steel. They may be cleaned and sterilized.

Maintenance

7.1.3 Air intake grille and filter

7.1.4 Heated vent port

NOTICE!

Hint!

Risk of material damage

 Serious damage to the freezer may result if the air intake is blocked. Check that there is no

obstruction of the airflow to the freezer. The air intake filter must also be cleaned regularly.

 Remove the filter from behind the grille by turning the thumbscrews ¼ turn and opening grille

downward. The filter should be washed in warm soapy water and left to air dry before
replacing.

The air intake grille must be cleaned regularly to keep it free from dust and debris. Under normal
conditions, clean the grille once every three months. If the area around the freezer is very dusty
or dirty, clean the grille more often.

 Brush the grille with a soft brush and, if a vacuum cleaner is available, vacuum the dust from

the grille.

There is an electrically-heated vent port in the freezer which must not be allowed to become
blocked or sealed off.

Over a period of a few weeks, depending on how often the freezer is being used, a small
mushroom of ice will form around the end of the vent port. If the vent port is allowed to become
blocked, a vacuum will be created when the door is closed. It will not be possible to open the door
or lift the lid until the vacuum has leaked away through the seal, which can take up to two hours
due to the high quality of the seals.

The vent port is located on the left-hand side of the freezers.

 If the door cannot be opened, clear the vent port by pressing the manual plunger on the

outside of the air vent.

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Premium -86 °C Freezers — Operating manual

123

7

1 Plunger 2 Cover

3 Freezer outer wall

7.1.5 Door or lid seal

Be sure to treat the door or lid seal with care. Avoid damaging this seal in any way. The freezer
cannot operate properly with a defective seal.

 It is advisable to wipe both the seal and the surface against which it seals with a soft dry cloth

once a month.

7.2 Routine maintenance

7.2.1 Lubrication

Every 12 months the outer door hinges and the handle mechanism should be lightly lubricated
using general-purpose oil or spray grease.

7.2.2 Defrosting

After an extended period of operation, defrosting may become necessary:

Risk of material damage

 Do not attempt to chip or scrape the ice with a sharp instrument. Allow the ice to melt

NOTICE!

naturally.

1. De-activate the alarm by switching the battery (alarm) switch (located behind the lockable
panel on the front of the freezer) to off (O).

2. Unplug the freezer from the mains/electrical supply.

3. Leave the innner and outer doors or lids open.

4. Allow the accumulated ice to melt.

5. Mop up the resulting water.

6. Dry and decontaminate the interior of the freezer.

7. When defrosting is complete, reconnect the freezer to the mains/electrical supply.

8. Turn the mains/power switch on (I) and re-activate the battery (alarm) switch.

Maintenance

7.2.3 Removing the inner doors

The inner doors of the freezer can be removed for defrosting and cleaning.

1. Fully open the outer door of the freezer.

2. Unscrew the door hinges.

3. Remove the inner door and set aside.

29

1

2

7

Maintenance

Premium -86 °C Freezers — Operating manual

Abb. 10: Screw hinge inner door

Fig. 10: Screw hinge inner door

1 Inner door 2 Door hinge screw x4

Repeat procedure for each door.

7.2.4 Replacing the inner door

1. Fully open the outer door of the freezer.

2. Reposition inner door in closed position.

3. Screw on door hinges.

4. If required, adjust by loosening the screws.

5. Close outer door.

7.2.5 Electrical components

Risk of personal injury

 During routine maintenance, care must be taken to avoid any damage to the gaskets and

WARNING!

 Failure to observe this safety warning will invalidate the warranty and could result in a

Lamps

Regularly check the indicator lamps:

 Press the ALARM TEST/MUTE key.

sealing grommets of these enclosures; also check the gaskets and sealing grommets
routinely to ensure their integrity. Should any damage or deformity be detected, the gasket
and/or sealing grommet must be replaced immediately.

dangerous situation.

All of the indicator lamps should illuminate, and the display should read 8888.

30

Alarms

Regularly check the alarm:

 Press the ALARM TEST/MUTE key.

The TEMP indicator should illuminate and the audible alarm should sound.

Premium -86 °C Freezers — Operating manual

Battery replacement

Risk of material damage

 There are no user controls behind any panels. The removal of any other part or panels from

NOTICE!

NOTICE!

the freezer by anyone other than a qualified, authorized Service Engineer may invalidate the
warranty.

Risk of material damage

 Use only a replacement battery of the correct type and part number.

 The battery must be fitted so the terminals correspond to the polarity labels on the electrical

panel.

The YUASA–NP6 V 2.8 Ah battery is mounted on the electrical panel. This is located behind the
right-hand base cover.

To replace the battery:

1. Switch off the mains/power switch and disconnect the mains/power supply.

2. Remove the side cover and the battery clamp securing the battery to the electrical panel.

3. Disconnect the battery terminals.

4. Install the new battery, fixing screws, and the side cover.

7

Maintenance

Be certain, when reconnecting the battery, to respect the correct polarity (red is + positive and

Hint!

black is – negative).

5. Reconnect the freezer to the mains/power supply and turn the mains/power switch on (I).

Fuses

Fuses must be replaced by a New Brunswick or Eppendorf approved service engineer. Contact
New Brunswick or Eppendorf Service.

31

Premium -86 °C Freezers — Operating manual

8 Troubleshooting

8 Troubleshooting

8.1 General errors

If you are experiencing a problem with your freezer, check the following troubleshooting guides
before contacting your New Brunswick or Eppendorf authorized Service technician.

8

8.2 Error messages

Troubleshooting

Symptom/
message

Door will not
open

Cause Remedy

1. The door handle is locked.

2. The heated vent port is
blocked.

1. Unlock the door handle.

2. Break up the ice in the vent port using
the plunger, (see Heated vent port on
p. 28).

If the door will not open:

 Call New Brunswick or Eppendorf

Service.

FILTER-CLEAN
LED lights up

• Filter is contaminated.  Clean the filter, (see Air intake grille

and filter on p. 28).

If the LED remains lit:

 Call New Brunswick or Eppendorf

Service.

Your electronically-controlled New Brunswick freezer incorporates the unique Systems
Monitoring And Reporting Technology (S.M.A.R.T. Plus™) self-diagnostic software to diagnose
faults in its electronic systems, its probes and/or its refrigeration system.

This table interprets error codes that may appear in the control panel display:

Symptom/
message

E-01

E-02

E-03 • This probe monitors the

E-04 Air-cooled condenser

Cause Remedy

• PT100 Probe 1 failure. This

probe, located inside the
freezer cabinet, indicates
cabinet temperature.

• Probe 2 failure. This probe

monitors the cascade
condenser.

 Call New Brunswick or Eppendorf Service

department.

 Call New Brunswick or Eppendorf Service

department.

 Call New Brunswick or Eppendorf Service

air-cooled condenser.

temperature too high:

1. Filter may be blocked.



2. Ambient temperature may be
too high.

If alarm continues to sound:

• Fan may have failed.

department.





1. Clean filter according to the instructions
(see Air intake grille and filter on p. 28).

2. Cool the room.



 Call New Brunswick or Eppendorf Service

department.

• Water supply not turned on,

insufficient flow, regulating
valve not opening or
defective (water-cooled
version only).

32

Premium -86 °C Freezers — Operating manual

A fan is required to cool the compressors on the water-cooled models.

Hint!

8.3 After a mains/power failure

If mains/power is interrupted, the POWER-FAIL indicator lamp (see Fig. 3 on p. 16), Item 3, will
illuminate. In addition, the audible alarm will sound and the display will flash at approximately
10-second intervals.

When mains/power is restored, both alarm and light will automatically be cancelled.

If mains/power has been interrupted for only a short time, the internal temperature of the freezer
will not have risen above the temperature setpoint (the user-set alarm threshhold), so normal
operation will be resumed immediately.

If the interruption was long enough for the internal temperature to rise above the temperature
setpoint, the TEMP-ALARM indicator will illuminate. If the internal temperature does not fall
below the temperature setpoint within the programmed time after mains/power was restored, the
audible alarm will sound again. The TEMP-ALARM indicator will extinguish when the internal
temperature reaches the High Alarm temperature set point.

8.4 Interior warming

8

If the lid or door is left open long enough for the internal temperature to rise above the
temperature setpoint, the same effects will be observed as described above regarding power
failure.

To minimize the risk of this happening, the lid or door should only be opened when necessary, for
a short period of time.

The upright freezers are fitted with internal doors which latch shut, minimizing temperature rise
when the outer door is opened. Chest freezers are fitted with inner insulating lids to ensure
efficient running of the freezer. The lids should remain fitted at all times when the freezer is
running.

Troubleshooting

33

Premium -86 °C Freezers — Operating manual

9 Technical data

9 Technical data

9.1 Specifications

9.1.1 Upright freezers specifications

Part No. U9260-000X* U9270-000X*

Internal Dimensions:

Height x Width x Depth

External Dimensions:

Height x Width x Depth

Capacity 410 Liters

Net Weight 235 kg

Lock Standard Standard

No. Compartments 55

Interior Stainless steel grade 304L

9

Technical data

Alarms High/Low temperature, power fail, battery low, filter clean, fault

Insulation Material Urethane foam

Remote alarm port Standard Standard

RS-485 interface Optional Optional

Refrigerants: High Stage Refrigerant: R404A / Low Stage Refrigerant: R508B

‡Power Consumption:

• 120 V electrical supply 540 Watts 590 Watts

• 208 - 230 V electrical

• 230 V electrical supply 450 Watts 490 Watts

Mains/Power Source and Current Rating

120 V, 60 Hz 16.5 A 16.5 A

208 - 230 V, 60 Hz 8 A 9 A

230 V, 50 Hz 5 A 6 A

Pull Down Time: From +25°C to -85°C (freezer empty; 230 V, 50 Hz electrical supply)

Model No. U410 U570

1265 x 550 x 575 mm

49.8 x 21.6 x 22.6 in

1915 x 800 x 852 mm

75.3 x 31.5 x 33.5 in

14.5 cubic feet

517 lb

540 Watts 590 Watts

supply

5.3 hours 5.5 hours

1265 x 765 x 575 mm

49.8 x 30.1 x 22.6 in

1925 x 1025 x 852 mm

75.8 x 40.3 x 33.5 in

570 Liters

20.0 cubic feet

265 kg

583 lb

Performance -50 °C to -86 °C at 32 °C maximum ambient operating temperature

Environmental Conditions All freezers use components tested to CE/UL specifications listed below:

• Indoor use

• Altitude up to 2000 m

• Ambient temperature range 10 °C to 32 °C

• Maximum relative humidity 80 % for temperatures up to 31 °C, decreasing linearly to

50 % relative humidity at 40 °C

• Mains/power supply voltage fluctuations not to exceed ± 10 % of the nominal voltage

• Installation category II

• Pollution degree 2

* X = 0 for 120 V, 2 for 208 - 230 V, or 1 for 230 V

‡ Freezer set to -80 °C, ambient 20 - 25 °C at rated mains/electrical supply

34

Premium -86 °C Freezers — Operating manual

Model No. U700

Part No. U9280-000X*

Internal Dimensions:

Height x Width x Depth

External Dimensions:

Height x Width x Depth

Capacity 700 Liters

Net Weight 280.5 kg

Lock Standard

No. Compartments 5

Interior Stainless steel grade 304L

Alarms High/Low temperature, power fail, battery low, filter clean, fault

Insulation Material Urethane foam

Remote alarm port Standard

RS-485 interface Optional

Refrigerants: High Stage Refrigerant: R404A / Low Stage Refrigerant: R508B

‡Power Consumption:

• 120 V electrical supply 663 Watts

• 208 - 230 V electrical supply 663 Watts

• 230 V electrical supply 663 Watts

Mains/Power Source and Current Rating

120 V, 60 Hz 16.5 A

208 - 230 V, 60 Hz 10 A

230 V, 50 Hz 10 A

Pull Down Time: From +25°C to -85°C (freezer empty; 230 V, 50 Hz electrical supply)

Performance -50 °C to -86 °C at 32 °C maximum ambient operating temperature

Environmental Conditions All freezers use components tested to CE/UL specifications listed below:

• Indoor use

• Altitude up to 2000 m

• Ambient temperature range 10 °C to 32 °C

• Maximum relative humidity 80 % for temperatures up to 31 °C, decreasing

linearly to 50 % relative humidity at 40 °C

• Mains/power supply voltage fluctuations not to exceed ± 10 % of the nominal

voltage

• Installation category II

• Pollution degree 2

1315 x 865 x 610 mm

51.8 x 34.1 x 24.1 in

1955 x 1120 x 865 mm

77.1 x 44.1 x 34.2 in

24.6 cubic feet

615 lb

5.3 hours

9

Technical data

* X = 0 for 120 V, 2 for 208 - 230 V, or 3 for 230 V

‡ Freezer set to -80 °C, ambient 20 - 25 °C at rated mains/electrical supply

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Premium -86 °C Freezers — Operating manual

9.1.2 Chest freezers specifications

Part No. U9230-000X* U9250-000X*

Internal Dimensions:

Height x Width x Depth

External Dimensions:

Height x Width x Depth

Capacity 340 Liters

Net Weight 205 kg

Lock Standard Standard

No. Compartments N/A N/A

Interior Stainless steel grade 304L

Alarms High/Low temperature, power fail, battery low, filter clean, fault

Insulation Material Urethane foam

Remote alarm port Standard Standard

RS-485 interface Optional Optional

9

Technical data

Refrigerants: High Stage Refrigerant: R404A / Low Stage Refrigerant: R508B

‡Power Consumption:

• 120 V electrical supply 430 Watts N/A

• 208 - 230 V electrical

• 230 V electrical supply 368 Watts 595 Watts

Mains/Power Source and Current Rating

120 V, 60 Hz 16.5 A N/A

208 - 230 V, 60 Hz N/A 8 A

230 V, 50 Hz 4.5 A 6 A

Pull Down Time: From +25°C to -85°C (freezer empty; 230 V, 50 Hz electrical supply)

Performance -50 °C to -86 °C at 32 °C maximum ambient operating temperature

Environmental Conditions All freezers use components tested to CE/UL specifications listed below:

Model No. C340 C660

760 x 760 x 590 mm

30 x 30 x 23.2 in

1075 x 1340 x 840 mm

42.3 x 52.7 x 33 in

12.0 cubic feet

451 lb

N/A 625 Watts

supply

3.8 hours 5.8 hours

• Indoor use

• Altitude up to 2000 m

• Ambient temperature range 10 °C to 32 °C

• Maximum relative humidity 80 % for temperatures up to 31 °C, decreasing linearly to

50 % relative humidity at 40 °C

• Mains/power supply voltage fluctuations not to exceed ± 10 % of the nominal voltage

• Installation category II

• Pollution degree 2

760 x 1470 x 590 mm

30 x 57.8 x 23.2 in

1075 x 2050 x 840 mm

42.3 x 80.7 x 33 in

660 Liters

23.3 cubic feet

280 kg

616 lb

36

* X = 0 for 120 V, 2 for 208 - 230 V, or 1 for 230 V

‡ Freezer set to -80°C, ambient 20 - 25 °C at rated mains/electrical supply

Premium -86 °C Freezers — Operating manual

10 Ordering Information

10 Ordering Information

10.1 Accessories

A number of accessories are available for New Brunswick's range of ultra-low temperature
freezers. Contact your local Eppendorf representative or distributor for details.

10.1.1 TCA-3 independent temperature monitor

The TCA-3 monitoring system is an independent temperature monitor with alarm, electronic chart
recorder, and auto-dialer that communicates via the internet for remote monitoring from anywhere
in the world. Ask your New Brunswick or Eppendorf sales representative for availability.

10.1.2 Auto-dialers

Auto-dialers can call a set of preprogrammed telephone numbers in the event of an alarm
condition and connect right into the freezer's remote alarm port.

10.1.3 Temperature probes

Additional Temperature Probes (such as the New Brunswick TCA-3 monitoring system) can be
installed upon request for an external alarm system or for validation.

10.1.4 Validation packages

Installation and operational qualifications are available.

10.1.5 External voltage stabilizers

External voltage stabilizers are available for all Premium Freezers models to modulate incoming
line voltage.

10.1.6 Padlock adapter kits

Padlock adapter kits allow up to two user-supplied padlocks to be secured to outer door handle
for extra security.

10.1.7 CO

10.1.8 Inventory racking systems

and LN2 back-up systems

2

These systems are available to temporarily protect the contents of the freezer against the
consequences of freezer failure or power failure. In an emergency, the system can inject either
liquid carbon dioxide or liquid nitrogen from a storage bottle. Carbon dioxide back-up systems will
maintain temperatures between -50 °C and -70 °C (subject to environmental conditions) for a
period of up to 48 hours, during which time the freezer can be repaired. Liquid nitrogen back-up
systems will maintain the freezer temperature down to -85 °C.

and LN2 back up systems can be retrofitted by the user. Contact your local New Brunswick

CO

2

or Eppendorf distributor for options available. Instructions are included in the kit.

A very comprehensive set of anodized aluminium racks is available. These are designed to
accommodate various sizes of boxes neatly, while giving maximum packing density in the
freezer. Stainless-steel shelves and water proof boxes as well as custom racking are also
available.

10

Ordering Information

10.1.9 Chart recorder

A chart recorder is available to provide a continuous record of the temperature inside the freezer
over a period of seven days. The record is presented on a circular chart.

The following items are available for all freezer models:

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Premium -86 °C Freezers — Operating manual

10

10.1.10 New Brunswick BioCommand SFI datalogging software (RS-485 interface)

Ordering Information

New Brunswick's BioCommand® SFI Track and Trend software is available to trend and archive
data from as many as 32 shakers, CO2 incubators, and/or freezers, simultaneously. This
PC-based software works with any OPC-compliant equipment with an RS-232 or RS-485 port.

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Premium -86 °C Freezers — Operating manual

11 Transport, storage and disposal

11 Transport, storage and disposal

11

11.1 Shut down

11.2 Transport

WARNING!

CAUTION!

11.3 Disposal



Switch the battery switch off before transporting or storing the equipment, (see Battery
backup switch on p. 25).

Risk of personal injury

Lifting and transporting the freezer without suitable equipments can result in crushing and other
injuries.

 Use a mechanical lifting equipment to load and unload the freezer.

Risk of material damage

Vibrations and impacts can cause the hermetically sealed compressors to slip out of their
suspension system.

 Do not tilt the equipment.

 Avoid vibrations and impacts.

Perform the following steps if relocation is necessary:

1. Remove all shelves, racks, and boxes.

2. Carefully move the freezer.

In case the product is to be disposed of, the relevant legal regulations are to be observed.

Information on the disposal of electrical and electronic devices in the European
Community:

Within the European Community, the disposal of electrical devices is regulated by national
regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic
equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, in the
business-to-business sphere, to which this product is assigned, may no longer be disposed of in
municipal or domestic waste. To document this, they have been marked with the following
identification:

Transport, storage and disposal

Because disposal regulations may differ from one country to another within the EU, please
contact your supplier if necessary.

In Germany, this is mandatory from March 23, 2006. From this date, the manufacturer has to offer
a suitable method of return for all devices supplied after August 13, 2005. For all devices supplied
before August 13, 2005, the last user is responsible for the correct disposal.

39

12

Premium -86 °C Freezers — Operating manual

12 Certificates

12 Certificates

Certificates

40

Premium -86 °C Freezers — Operating manual

Index

Index

A

Accessories .....................................................................37

Adjusting the shelf ...........................................................14

Air intake..........................................................................28

Alarm ...............................................................................25

Alarm activation ...............................................................21

Alarm test/mute key .........................................................25

Audible alarm.............................................................16, 25

Auto-dialers......................................................................37

Auto-reset ........................................................................12

Automatic reset................................................................12

B

Battery activation .............................................................21

Battery backup switch......................................................25

Battery replacement.........................................................31

C

C760 Specifications .........................................................36

Caution, explanation of ......................................................5

CFC ...................................................................................6

Chart recorder..................................................................37

Chest freezer, front view ....................................................9

Cleaning.....................................................................28, 29

Clearance ........................................................................13

CO2 back-up system........................................................37

Control panel ...................................................................16

Copyright ...........................................................................2

Custom-made racks.........................................................37

D

Danger, explanation of .......................................................5

Defrosting ........................................................................29

Disposal ...........................................................................39

Door seal .........................................................................29

Door/lid stuck ...................................................................22

E

Electrical connection........................................................20

Electrical requirements ....................................................20

Error codes ......................................................................32

External monitoring..........................................................26

External voltage stabilizer................................................37

F

Factory-set temperature...................................................21

Fail, mains/power.............................................................33

H

Hazard symbols .................................................................5

HCFC................................................................................. 6

HFC ................................................................................... 6

I

Ice in vent .................................................................. 22, 28

Indicator lights ................................................................. 11

Inner door, installing ........................................................ 30

Inner door, removing........................................................ 29

Inspection of boxes.......................................................... 10

Installing inner door ......................................................... 30

Intended use...................................................................... 7

L

Lid/door stuck .................................................................. 22

LN2 back-up system ........................................................37

Lock key .......................................................................... 22

Lockable panel ................................................................ 21

M

Mains/power failure.......................................................... 33

Mains/power supply.........................................................20

Manual conventions........................................................... 5

min.....................................................................................6

Monitoring.................................................................. 26, 38

Moving freezer................................................................. 39

N

Notice, explanation of ........................................................ 5

P

Packing list verification ....................................................10

Panel, removing...............................................................20

Part numbers................................................................... 37

Plunger ......................................................................22, 28

Power cord....................................................................... 20

Power fail ......................................................................... 21

Power failure....................................................................25

Power supply/mains......................................................... 20

Programming................................................................... 22

R

Racks............................................................................... 37

Recorder pens................................................................. 37

Recorder, chart ................................................................37

Remote control .................................................................. 9

Remote monitoring ....................................................26, 38

Removing inner doors .....................................................29

Removing lockable panel ................................................20

Removing upright freezer lock barrel...............................14

Rise in temperature .........................................................33

rpm ....................................................................................6

RS-485 computer interface.............................................. 38

Index

41

Index

Premium -86 °C Freezers — Operating manual

S

S.M.A.R.T. Plus..........................................................16, 32

Safety.................................................................................7

Set temp key ....................................................................22

Setpoint, temperature ......................................................33

Setting the operating temperature ...................................22

Shelf, adjusting ................................................................14

Specifications, C585 ........................................................36

Specifications, U410 ........................................................34

Specifications, U700 ........................................................35

Symbols used ....................................................................5

T

Temp alarm ......................................................................33

Temperature alarms.........................................................16

Temperature probes.........................................................37

Temperature setpoint.................................................22, 33

Testing alarm ...................................................................30

Testing alarm monitoring socket ......................................21

Testing indicator lamps ....................................................30

Trademarks........................................................................2

Troubleshooting ...............................................................32

Turning on the equipment ................................................20

U

U570 Specifications .........................................................34

U700 Specifications .........................................................35

Unpacking boxes .............................................................10

Upright freezer, front view ..................................................9

Upright freezer, side view ..................................................9

Using this manual ..............................................................5

V

Vacuum effect ..................................................................22

Validation packages.........................................................37

Vent............................................................................22, 28

W

Warming...........................................................................33

Warning, explanation of .....................................................5

42

Your local distributor for New Brunswick products: www.nbsc.com/ContactUs

Eppendorf AG · 22331 Hamburg · Germany · Tel: [phone] · Fax: [phone] · E-mail: [e-mail]

New Brunswick Scientific Europe B.V. · Nijmegen · The Netherlands · Tel: [phone] · Email: [e-mail]

Eppendorf North America, Inc. · Hauppauge, N.Y. USA · Tel: [phone] · [phone] · E-mail: [e-mail]

Application Support Europe, International: Tel: [phone] · E-mail: [e-mail]

North America: Tel: [phone] menu option 2 · E-mail: [e-mail]

Asia Pacific: Tel: [phone] · E-mail: support_asiapacifi[e-mail]

New Brunswick Scientific · 175 Freshwater Blvd., Enfield, CT 06082-4444 · USA