Eppendorf HPLC adapter User Manual

HPLC adapter — Instructions for use

This document supplements the instructions for use of the Centrifuge, but does not replace them.
Therefore, also read the instructions for use. The current version can be found online at
www.eppendorf.com

Instructions for use

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Product description

The HPLC adapters are designed for centrifugation of HPLC vials with max. 1.5 mL filling volume
in the F-45-18-17-Cryo rotor for Centrifuge 5430/5430 R and Centrifuge 5702/5702 R/5702 RH.

Safety notes

 Prior to using HPLC vials, observe manufacturer specifications on resistance to

centrifugation (max. g-force).

 Do not exceed the maximum tube length of 33 mm (without cap) so the tubes can be

optimally supported by the adapters.

 Do not exceed a g-force of 3,500 x g at a max. load of 9.5 g per rotor bore (adapter + tube +

contents). At a load of up to 8.7 g per rotor bore, the adapters can be centrifuged at max.
g-force (rcf)/rotational speed (rpm).

 Load the rotor symmetrically. When doing so, follow the loading instructions included in the

instructions for use of the centrifuge.

Cleaning and Disinfection

Use a mild cleaning agent for cleaning.
Use alcohol (ethanol, isopropanol) or disinfectants containing alcohol for the disinfection.

The HPLC adapter can be autoclaved (121°C, 20 min).

Hint!

Technical data

For the Centrifuge 5430/5430 R, ensure that the g-force displayed during switch-over is
standardized to the corresponding rotor without adapter and, for the Centrifuge 5702/5702 R/
5702 RH, standardized to the A-4-38 rotor. You can achieve the following maximum g-forces (rcf)
at maximum speed (rpm) when adapters are used:

Rotor, centrifuge Adapters Max. centrifugation

F-45-18-17-Cryo,
Centrifuge 5430/5430 R

F-45-18-17-Cryo,
Centrifuge 5702/5702 R/5702 RH

without adapter
HPLC adapter

without adapter
HPLC adapter

radius (cm)

9.4

8.3

9.4
8,3

Max. g-force (rcf)
/ speed (rpm)

8,320 / 8,900
7,350 / 8,900

2,030 / 4,400
1,800 / 4,400

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HPLC adapter — Instructions for use

Max. allowed tube lengths without cap 33 mm

Adapter bore (Ø) 11.8 mm

Material Polypropylene

You can calculate the g-force (rcf) for a special adapter using the following formula according to
DIN 58 970:

rcf = 1.118 * 10
Example: The max. centrifugation radius of the HPCL adapter is 8.3 cm in the F-45-18-17-Cryo

rotor. A max. g-force of 3,500 x g is reached at 6142 rpm.

Ordering information

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* n2 * r

, n: rotational speed (rpm), r

max

: max. centrifugation radius (cm)

max

Order No.
(International)

Order No.
(North America)

Description

HPLC adapter

5427 708.006 5427708006

18 pcs.

5427 708.960-00/0210

Your local distributor: www.eppendorf.com/worldwide

Eppendorf AG · 22331 Hamburg · Germany · Tel: [phone] · Fax: [phone]

Eppendorf North America, Inc. · 102 Motor Parkway, Suite 410 · Hauppauge, NY 11788-5178 · USA

Tel: [phone] · Toll free phone: [phone] · Fax: [phone] · E-Mail: [e-mail]

Europe, International: Tel: [phone] · E-Mail: [e-mail]

North America: Tel: [phone] ext. 2258 · E-Mail: [e-mail]

Asia Pacific: Tel: [phone] · E-Mail: [e-mail]

E-Mail: [e-mail]

Application Support

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