o
r useµPlate G0.5
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Eppendorf® μPlate G0.5
Instructions for use
Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.
Trademarks
Eppendorf
Hamburg, Germany.
KimWipe
Excel
Trademarks are not marked in all cases with ™ or
®
and the Eppendorf Logo are registered trademarks of Eppendorf AG,
®
is a registered trademark of Kimberly-Clark Corporation, Irving, TX, USA.
®
is a registered trademark of Microsoft Corporation, Redmond, WA, USA.
®
in this manual.
6144 900.209-01/062013
Table of contents
Eppendorf
®
µPlate G0.5
English (EN)
Table of contents
1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.1 Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.3 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.3.1 Personal injury. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.3.2 Damage to device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.2 System requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
5.1 Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
5.1.1 Nucleic acid quantification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
5.2 Preparing measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
5.2.1 Defining the blank value procedure . . . . . . . . . . . . . . . . . . . . . . . 10
5.2.2 Average blanking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
5.2.3 Individual Blanking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
5.2.4 Sample ID function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
5.2.5 Show Raw Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
5.2.6 Editing sample types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.2.7 Loading samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.2.8 Loading samples using the pipetting aid . . . . . . . . . . . . . . . . . . . 17
5.2.9 Pipetting with multi-channel pipettes . . . . . . . . . . . . . . . . . . . . . . 17
5.2.10 Pipetting with single-channel pipettes . . . . . . . . . . . . . . . . . . . . . 17
5.3 Performing a measurement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.4 Quality control of the µPlate G0.5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.4.1 Average blanking out of range (CV ≥ 10 %) . . . . . . . . . . . . . . . . . 21
5.4.2 Ending the blanking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Table of content s
Eppendorf
4
®
µPlate G0.5
English (EN)
6 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
6.1 Working with the µPlate G0.5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7.2 Disinfection/Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
8 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
8.1 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
8.2 Additional parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
9 Calculations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
9.1 Calculating the nucleic acid concentration . . . . . . . . . . . . . . . . . . . . . . . . . . 26
9.1.1 Example . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
9.2 Blank values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
9.2.1 Determining the average blank value . . . . . . . . . . . . . . . . . . . . . . 27
9.2.2 Determining the individual blank values. . . . . . . . . . . . . . . . . . . . 27
9.3 Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
9.3.1 Calculations based on an average blank value . . . . . . . . . . . . . . . 27
9.3.2 Calculations based on an individual blank value. . . . . . . . . . . . . . 28
10 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
11 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
11.1 Shipping the µPlate G0.5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Operating instructions
Eppendorf
®
µPlate G0.5
English (EN)
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time.
Please also note the operating instructions for the accessories, if applicable.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. For the current version, please refer
to our webpage www.eppendorf.com/worldwide
www.eppendorfna.com
(North America).
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and
danger levels:
1.2.1 Danger symbols
Biohazard Explosion
Electric shock Toxic substances
Hazard point Material damage
(international) or
5
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WAR NIN G Can lead to severe injuries or death.
CAUTION Can lead to light to moderate injuries.
ATTENTION May lead to material damage./Paragraph
1.3 Symbols used
Depiction Meaning
1.
2.
Actions in the specified order
Actions without a specified order
• List:
Text Display or software texts
Additional information
Product description
Eppendorf
6
®
µPlate G0.5
English (EN)
2 Product description
2.1 Introduction
The µPlate G0.5 is a microvolume plate for quantifying small nucleic acid volumes (2 µL)
in the absorbance mode of PlateReader AF2200.
In the µPlate G0.5, 16 different samples can be analyzed at the same time during one
measuring procedure. After the measurement, which is controlled by the PlateReader
AF2200 software, the nucleic acid content is calculated automatically and the purity is
checked using the 260/280 ratio. The results will be output in an Excel spreadsheet. A
blank measurement, as well as a reference wavelength ratio at 340 nm at the start of the
measuring procedure, acts as a quality control check for the entire plate and displays any
pipetting or cleaning errors which may be present. The plate was specially developed for
the unique requirements of research laboratories, where various types of small sample
volumes are quantified.
2.2 Delivery package
Quantity Description
1µPlate G0.5
1 Pipetting template
1 Instructions for use
1Storage case
The µPlate G0.5 is compatible with the Eppendorf PlateReader AF2200.
The µPlate G0.5 is not included in the Eppendorf PlateReader AF2200 delivery package.
Safety
Eppendorf
®
µPlate G0.5
English (EN)
3Safety
3.1 Intended use
The µPlate G0.5 is designed for use in molecular biology, biochemistry and cell biology
research laboratories. The µPlate G0.5 is exclusively intended for use indoors. The µPlate
G0.5 is designed as an auxiliary aid of the PlateReader AF2200 for laboratory
measurements for quantifying small nucleic acid volumes (2 L) in the absorbance mode.
3.2 User profile
The device and accessories may only be operated by trained and skilled personnel.
Before using the device, read the operating manual carefully and familiarize yourself with
the device's mode of operation.
3.3 Warnings for intended use
3.3.1 Personal injury
WARNING! Damage to health from toxic, radioactive or aggressive chemicals
as well as infectious liquids and pathogenic germs.
Observe the national regulations for handling these substances, the biological
security level of your laboratory, the material safety data sheets and the
manufacturer's application notes.
Wear personal protective equipment.
For comprehensive regulations about handling germs or biological materials
of the risk group II or higher, please refer to the "Laboratory Biosafety
Manual" (source: World Health Organisation, Laboratory Biosafety Manual, in
its current version).
7
WARNING! Damage to health due to contaminated device and accessories.
Decontaminate the device and the accessories before storage and shipping.
Safety
Eppendorf
8
English (EN)
®
µPlate G0.5
3.3.2 Damage to device
NOTICE! Damage from the use of aggressive chemicals.
Do not use any aggressive chemicals on the device or its accessories, such as
strong and weak bases, strong acids, acetone, formaldehyde, halogenated
hydrocarbons or phenol.
If the device has been contaminated by aggressive chemicals, clean it
immediately using a mild cleaning agent.
NOTICE! Corrosion due to aggressive cleaning agents and disinfectants.
Do not use corrosive cleaning agents, aggressive solvents or abrasive
polishes.
Do not incubate the accessories in aggressive cleaning agents or disinfectants
for longer periods.
Installation
Eppendorf
®
µPlate G0.5
English (EN)
4 Installation
4.1 Preparing installation
Store the transport packaging and packing material for future safe transport or
storage.
Use the details included in the delivery package to check that the delivery is complete .
Check all parts for any transport damage.
4.2 System requirements
The following items are required for a measurement with the µPlate G0.5:
• An Eppendorf PlateReader AF2200
• A computer with the PlateReader AF2200 software
Make sure that the following absorbance filters are installed in the filter slide of your
device:
• Position 1: 260 (5) nm
• Position 2: 280 (5) nm
• Position 3: 340 (10) nm
The PlateReader AF2200 and µPlate G0.5 may only be used at room temperature
under normal laboratory conditions.
9
10
Operation
Eppendorf
®
µPlate G0.5
English (EN)
5Operation
5.1 Applications
5.1.1 Nucleic acid quantification
For the quantification procedure in the µPlate G0.5, a sample volume of 2 L is sufficient
in order to achieve accurate results. The absorbance of the nucleic acid samples is
measured at 260 nm. The optical path length of the µPlate G0.5 is 0.5 mm. To assess the
purity of the nucleic acid, an additional measurement is conducted at 280 nm to display
any proteins that may be present in the sample. A 260/280 ratio between 1.8 and 1.9 is
acceptable for pure nucleic acids. A ratio < 1.8 may show that the sample contains
proteins or other impurities. If this is the case, additional purification steps are
recommended.
5.2 Preparing measurements
5.2.1 Defining the blank value procedure
The user can select from two options: determining the average blank value ("average
blanking") and determining the individual blank values ("individual blanking", set as
default).
5.2.2 Average blanking
To determine the average blank value, select the wells to be used for blanking. To do so,
drag a border around the corresponding sample positions in the plate preview. We
generally recommend carrying out the blank measurement with all 16 sample positions.
However, at least two wells are required to calculate an average value which will then be
used for the blank value correction of all measured samples.
To ensure reliable measuring results, the OD results measured must have a CV of < 10%
(CV= Coefficient of variation).
Abb. 5-1:"Start Blanking" button
Fig. 5-1: "Start Blanking" button
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