Eppendorf FemtoJet User Manual

FemtoJet

®

Operating Manual

Your local distributor:
www.eppendorf.com/worldwide

Eppendorf AG
22331 Hamburg · Germany
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]

Eppendorf North America, Inc.
One Cantiague Road · P.O. Box 1019
Westbury, N.Y. 11590-0207 · USA
Tel. [phone]
Toll free phone [phone]
Fax [phone]
E-Mail: [e-mail]

Application Support

Europe, International:
Tel. [phone]
E-Mail: [e-mail]

North America:
Tel. [phone] ext. 2258
E-Mail: [e-mail]

Asia, Pacific:
Tel. [phone]
E-Mail:
support_AsiaPacifi[e-mail]

eppendorf® is a registered trademarkPrinted in Germany

Eppendorf Offices

AUSTRALIA / NEW ZEALAND

Eppendorf South Pacific Pty. Ltd.
Tel. [phone]
Fax [phone]
E-mail: [e-mail]
Internet: www.eppendorf.com.au

AUSTRIA

Eppendorf Austria
Tel. [phone]
Fax [phone]
E-Mail: offi[e-mail]
Internet: www.eppendorf.at

BRAZIL

Eppendorf do Brasil Ltda.
Tel. [phone]
Fax [phone]
E-Mail:
[e-mail]
Internet: www.eppendorf.com.br

CANADA

Eppendorf Canada Ltd.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorfna.com

CHINA

Eppendorf China Ltd.
Tel. [phone]
Fax [phone]

E-Mail: [e-mail]

Internet: www.eppendorf.cn

GERMANY

Eppendorf Vertrieb
Deutschland GmbH
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.de

INDIA

Eppendorf India Limited
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.co.in

ITALY

Eppendorf s.r.l.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.it

JAPAN

Eppendorf Co. Ltd.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.jp

NORDIC

Eppendorf Nordic Aps
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.dk

SPAIN

Eppendorf Ibérica S.L.U.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.es

SWITZERLAND

Vaudaux-Eppendorf AG
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.ch

UNITED KINGDOM

Eppendorf UK Limited
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.co.uk

USA

Eppendorf North America
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorfna.com

OTHER COUNTRIES

Internet:
www.eppendorf.com/worldwide

FRANCE

EPPENDORF FRANCE S.A.R.L.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.fr

SOUTH & SOUTHEAST ASIA

Eppendorf Asia Pacific Sdn. Bhd.
Tel. [phone]
Fax [phone]
E-Mail:

asiapacifi[e-mail]

Internet: www.eppendorf.com.my

Contents

1 Safety precautions and application

2 Device description

2.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.2 Short description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3 Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Mode of operation

3.1 Display, variable regulators and keypad . . . . . . . . . . . . . . . . 12

3.1.1 Setting the injection parameters . . . . . . . . . . . . . . . . . . . . . . 12

3.1.2 Variable regulators. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.1.3 Keypad. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.2 Hand control / foot control . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3.3 Connecting the capillary holder . . . . . . . . . . . . . . . . . . . . . . . 18

3.4 Information on working practices. . . . . . . . . . . . . . . . . . . . . . 19

3.4.1 Compensation pressure p

3.4.2 Injection pressure pi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

3.4.3 Injection time ti . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

3.4.4 Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

3.4.5 Manual or automatic injection . . . . . . . . . . . . . . . . . . . . . . . . 23

3.4.6 Constant pressure/constant working pressure . . . . . . . . . . . 23

3.4.7 General note . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

3.5 The first injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

3.6 Connecting an Eppendorf Micromanipulator . . . . . . . . . . . . . 25

3.7 Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

3.7.1 Changing the capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

3.7.2 Setting the pressure unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

3.7.3 Switching on/off the loudspeaker. . . . . . . . . . . . . . . . . . . . . . 27

3.7.4 Switching on/off the background illumination of the display . 27

3.7.5 Constant pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

3.7.6 Dewatering the pressure accumulator. . . . . . . . . . . . . . . . . . 28

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

. . . . . . . . . . . . . . . . . . . . . . . . . . 21

c

. . . . . . . . . . . . . . . . . . . 3

4 Cleaning and Service

4.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

4.2 Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

4.3 Decontamination prior to shipping . . . . . . . . . . . . . . . . . . . . . 29

4.3 Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

No part of this publication may be reproduced without the prior permission of the copyright owner.

Copyright© 2006 by Eppendorf AG, Hamburg

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

B 5247 900.011-12/0108

Contents

5 Troubleshooting

5.1 Potential errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.1.1 Self-test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.2 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.3 Error messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6 Technical data

7 Ordering information

7a Ordering information for North America

8 Index

Appendix A

A.1 Description of interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

A.2 Commands and queries . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

EC Conformity Declaration

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

Operation via a computer

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

. . . . . . . . . . . . . . .44

. . . . . . . . . . . . . . . . . . . . . . . . . . 49

2

1 Safety precautions and application

Observe operating instructions!

●

The device is only designed for use in the research lab!

●

●

The FemtoJet is a lab device. It may only be operated by appropriately
qualified lab personnel.

●

Only use the device for microinjection under observance of all relevant
legal regulations!

Before plugging in the device, please compare your voltage

●

requirements with the specifications on the identification plate!

The unit may not be connected to a socket that is not grounded!

●

Use the appropriate mains cable.

Electrical connections may be effected only when the device is

●

switched off!

●

The unit may be opened only by authorized service personnel!
Potentially lethal voltage inside the unit.

Do not use the unit if damaged. This applies in particular when the

●

mains cable is damaged.

If the unit is not used in accordance with purpose for which it is

●

intended, its protective function may be impaired.

●

●

In the case of independently-manufactured capillaries, ensure that the

●

outer diameter of the capillary corresponds to the specifications of the
capillary grip head.

●

In the case of extended periods of non-use, the unit should be
depressurized using the key and switched off before being
stored!

Caution!

Take care when mounting glass capillaries or tools!
Mounting must always take place without the use
of pressure! Capillaries which have been mounted
incorrectly may break loose under pressure. Never
point mounted capillaries at persons.

Caution!

Take care when using glass capillaries! During
mounting or when placed upon a hard surface, the
glass capillaries may break and glass splinters may
cause injury. It is therefore essential to wear eye
protection!

3

1 Safety precautions and application

Use the unit only for the described purpose! The unit and in particular

●

the high pressure of the Clean function may not be used for purposes
other than microinjection!

●

Transfer

If the device is passed on to someone else, please include the
instruction manual.

●

Disposal

In case the product is to be disposed of, the relevant legal regulations
are to be observed.

Information on the disposal of electrical and electronic devices in
the European Community

The disposal of electrical devices is regulated within the European
Community by national regulations based on EU Directive 2002/96/EC
on waste electrical and electronic equipment (WEEE).
According to these regulations, any devices supplied after 13.08.05 in
the business-to-business sphere, to which this product is assigned,
may no longer be disposed of in municipal or domestic waste.
They are marked with the following symbol to indicate this.

As disposal regulations within the EU may vary from country to
country, please contact your supplier if necessary.

●

Use only original Eppendorf accessories. Only original accessory
parts may be used. The only exceptions are auxiliary pieces of
equipment recommended by the manufacturer. Otherwise all claims
under guarantee and liability become void immediately. Use other
accessories only if you have a certificate from Eppendorf AG
confirming technical safety in use.

The responsibility for the correct functioning of the device passes

●

onto the owner or user if the device is maintained or serviced
improperly by persons not belonging to the Eppendorf service team or
if used for a purpose other than that intended. Eppendorf AG is not
liable for damage occurring from non-observance of these notes.
Guarantee and conditions of liability of the terms of sale and delivery
of Eppendorf AG are not expanded by the above notes.

●

The Eppendorf AG reserves the right to make technical alterations to
this device!

4

2 Device description

2 Device description

The FemtoJet enables minute quantities of liquid to be injected into cells.
The relevant parameters (injection pressure pi and injection time ti)

are set using the variable regulators. The key or connected hand or
foot control or the INJECT key of the connected micromanipulator are

used to trigger these two parameters. If no injections are being performed,
compensation pressure pc is applied permanently.

5

2 Device description

2.1 Overview

1

2

3

4

1

Display

– Displays relevant parameters during injection or setting

of parameters.

– Guides operator through functions.

2

Keypad

– To trigger actions.
– To call up the function menu.
– To switch to and from Standby mode.

3

Variable regulators

– To set parameters injection pressure pi, injection time ti and

compensation pressure pc.

– To select functions and parameters in Function mode.

4

Tube connection

– To connect the injection tube to the device via the bayonet joint.

6

2 Device description

Standard accessories:

1 Injection tube, 2 m
1 Universal capillary holder with grip head for capillary diameters
of 1.0 mm and adapter for Femtotips
1 Hand control for triggering injection and the Clean function
1 Femtotips, Femtotips II and Microloader
1 Mains cable
1 Operating manual

Optional accessories:

1 Foot control for triggering the Inject function
1 Connecting cable (Interface Cable 5171) to the Micromanipulator 5171
or InjectMan





7

2 Device description

2.2 Short description

Display

Display of all relevant parameters and information.

Function keys

Clean

To apply the maximum pressure for cleaning the capillary
to the tube outlet.

Auto / Manu

To switch between manual and automatic time-controlled
injection.

Count

To reset to zero the counter for injections which have been
actuated.

Inject

To execute automatic or manual injection.

Menu

To switch the device to menu control; selected functions
may be executed.

Standby

When the key is pressed briefly:
To switch the device between operating mode and standby
mode.
When the key is held down:
As above, but also deairs the pressure accumulator.

8

2 Device description

Variable regulator

In the normal operating mode, the parameters for injection are set by the
three variable regulators. The arrangement of the variable regulators
corresponds to the arrangement of the parameters in the two bottom lines
of the display:
From left to right: injection pressure pi, injection time ti and compensation
pressure p
In other operating modes, the variable regulators have either the
significance indicated in the display or no significance at all.

Tube connection

The injection tube is connected to the device via a bayonet joint.

.

c

9

2 Device description

2.3 Startup

Diagram of rear of device

– Electrical connection is effected via the mains socket with integrated

mains switch (1).

3
3

2

1

– Connection to an Eppendorf manipulator designed for injection is

effected via the 15-pin interface (2).

– Optional switches (e.g. foot control, hand control) or a computer can

be connected to the 9-pin interfaces.

Switching on the device

Set the mains switch on the rear of the device to "I". The unit is switched
on, the display lights up, the unit runs through a warm-up routine and is
then ready for operation. The injection mode is shown in the display.

The injection tube may not be connected when switching on and during
the initialization phase.

After being switched on, the unit runs through an initialization phase and
supply pressure is built up. This takes approximately three minutes.

10

2 Device description

The drainage valve of the pressure cylinder is switched several times at
the beginning of the initialization phase. The pressure cylinder is also drai­ned every 3 hours when switched on. This results in a certain noise level
following a series-connected reminder signal.

Switching off the device

If it is not going to be used for an extended period, the device should

be depressurized via the switch-off routine using the key. This
guarantees a long service life of the device (see Section 3.1.3).

The switch-off routine ensures that the system is dewatered and deaired
correctly. Do not disconnect the device from the mains supply until
dewatering and deairing have taken place. If only the mains switch is
actuated, the existing pressure remains in the system.

11

3 Mode of operation

3 Mode of operation

3.1 Display, variable regulators and keypad

Before operating the device for the first time, users should read this
section carefully. This section describes operation of the device only.
Handling of the injection capillaries is described in Section 3.3.

AUTO n= 0

pi= 150 hPa

pi[hPa] ti[s] pc[hPa]

150 0.2 50

AUTO n= 0

pi= 215 PSI

pi[PSI] ti[s] pc[PSI]

2.15 0.2 0.72

Display

The display shows the following:

1st line:
injection mode AUTO or MANU,
counter n for number of injections
performed

2nd line (bold):
The most-recently set parameter or
relevant parameter

Bottom lines:
Parameters which affect injection

Compensation pressure pc which is
applied permanently in the normal
mode is displayed as an actual
measured value.

The arrangement of the three
parameters shown, p
corresponds to the arrangement of
the variable regulators.

, ti, pc,

i

3.1.1 Setting the injection parameters

The injection parameters have to be adapted to the injection capillaries
used and the cell type and liquid which are to be injected.

The following injection parameters can be set using the variable
regulators:

Injection pressure pi 0; 5 – 6,000 hPa (0.00; 0.07 – 87.0 psi)
Injection time ti0.1 – 99.9 s
Compensation pressure pc0; 5 – 6,000 hPa (0,00; 0.07 – 87.0 psi)

The settings are in increments of 1 hPa/0.1 s. The display can show hPa
or psi, as required. The setting and internal pressure conversion are
always performed in hPa. Rounding for the display in psi can result in
increments of 0.01 to 0.02 psi.
The display of pressure in psi varies according to the size of the value
(e.g.: 9.99 may also appear in the display as 10.0).

12

3 Mode of operation

Slight deviations between the nominal value (specified value) and
displayed compensation pressure pc (actual value) may occur for
technical reasons. The relevant value is the actual value measured.

The following settings are used as a
basis for initial injection experiments
using Femtotip

pileft-hand variable regulator: 150 hPa
ti central variable regulator: 0.5 s
pcright-hand variable regulator: 50 hPa



/ Femtotip II:

Variable regulators:

Display:

AUTO n= 0

pc= 50 hPa

pi[hPa] ti[s] pc[hPa]

150 0.5 50

3.1.2 Variable regulators

The variable regulators are set so that when they are turned slowly, the
values are changed by 1 hPa / 0.1 second per notch (0.01 – 0.02 psi).
If the regulators are turned faster, the values are altered in larger
increments.

13

3 Mode of operation

3.1.3 Keypad

Inject

Performs automatic or manual injection.
Automatic injection is controlled via the
injection time ti, and the injection
pressure pi is used. In the second line,
injection time ti is counted down from the
nominal value.

Manual injection is performed for as long as the key is held down.
Injection pressure pi is used. In the second line, injection time ti is counted
upwards from 0.0.

If the FemtoJet is connected to the Eppendorf Micromanipulator
InjectMan NI 2, 5171 or Inject Man, the injection movement of the

micromanipulator is also started via the key. Both units then
combine as a system to perform semi-automatic microinjection.
Alternatively, the INJECT key of the connected micromanipulator or the

hand/foot control can be used.
If synchronization is set to "limit" (SYNC = LIMIT) in the INJECT function

of the micromanipulator, semi-automatic injection can only be triggered
via the micromanipulator.

Clean

The maximum available pressure for
cleaning is applied at the tube outlet for
as long as the key is held down. Once the
key is released, the pressure drops to
compensation pressure p
pressure curve is shown in the form of
a bar.

again. The

c

AUTO - INJECT n= 0

0.4 s

pi[hPa] ti[s] pc[hPa]

150 0.6 50

CLEAN n= 0

≡≡≡≡≡≡≡≡≡≡≡≡≡≡≡_____

pi[hPa] ti[s] pc[hPa]

150 0.2 50

14

3 Mode of operation

Auto / Manu

To switch between manual and
automatic, time-controlled injection.

In the case of manual injection, time
is counted upwards from 0.0 seconds.
The parameter t
bottom two lines in order to emphasize
that the time cannot be set via the
variable regulators in this case.

Count

To reset to zero the counter for injections
which have been actuated.

Menu

To switch the device to menu control;
selected functions may be executed.

is not displayed in the

i

MANU - INJECT n= 0

0.4 s

pi[hPa] pc[hPa]

150 50

In the first line, FUNC indicates that the
device is in the function menu and shows
which function number is active.
The second line contains the function
description (e.g. "Select pressure unit").

The third line requests an action (e.g.
press the key to change the
pressure unit). Current status: [hPa]

The two bottom lines show information
about which action is assigned to which
variable regulator (e.g. other functions
can be selected using left-hand variable
regulator p

).

i

FUNC 1
Select pressure unit
INJECT = PSI [hPa]

Tu r n pi to
select function

15

3 Mode of operation

Standby

in the case of a brief interruption

When key is pressed briefly:
The device switches to the Standby
mode

without

of the display switches off. "STANDBY"
appears in the display with the suffix
"Supply pressure = rxyz" as a code for
system pressure ps being maintained.

Application:
For temporary interruptions or use of

as a Reset function.

When key is pressed again:
The device is reactivated and runs
through a short warm-up routine.
This lasts approximately one minute.

Standby

with deairing of pressure accumulator

Hold down the key (approx. 2 seconds;
the display moves to "STANDBY"):

The device switches to the Standby
mode. The pressure accumulator is
completely deaired, the illumination of
the display switches off. "STANDBY"
appears in the display with the note
"Supply pressure = x" (x ~ 0).

A noise can be heard at this stage.

Application:
For correct shutdown, with deairing of the
entire pressure system in the event of
longer periods of non-use or standstill
(weekend or storage) with subsequent
switch-off using the mains switch.

Press the key again:
The device is reactivated and runs
through the warm-up routine.
This takes approximately three minutes.

deairing. The illumination

STANDBY

Supply pressure 6789

Hold standby

to exhaust

STANDBY

Supply pressure 0

16

3 Mode of operation

Reset

To reset the device to the initial mode by
pressing the key briefly or via the
mains switch.

The pressure accumulator is not deaired.

3.2 Hand control / foot control

The functions of the hand/foot control
correspond to the functions of the

and keys on the

FemtoJet



.

The function of the foot control
corresponds to the key.

17

3 Mode of operation

3.3 Connecting the capillary holder

Universal capillary holder

The universal capillary holder is used to locate the injection capillaries.
Prefabricated Eppendorf Femtotips / Femtotips II (6) are screwed into
the universal capillary holder using the adapter (5). To do so, the adapter
for Femtotips (5) is first screwed into the front rotatable knurled screw
(4) and then the Femtotip / Femtotip II is attached. If self-pulled
capillaries are to be attached, the grip head 0 (7) needs to be screwed
loosely into the front rotatable knurled screw (4). The self-pulled capillary
is pushed into the front opening of the grip head through the two O-rings
and fixed in position by tightening the grip head.

12 3 4 5 6

7

Universal capillary holder

1Tube connection
2 Rear knurled screw
3Tension piece
4Front knurled screw, rotatable
5 Adapter for Femtotips
6Femtotip / Femtotip II
7Grip head 0, plastic, with two O-rings and sealing washer



(microcapillaries for microinjection)

Connecting the universal capillary holder

The two-meter-long injection tube is connected to the switched on
FemtoJet (1) via the bayonet joint (2). The injection tube (3) is screwed
to the rear knurled screw (4) of the universal capillary holder. Femtotips
Femtotips II are fitted at the front end of the universal capillary holder (5)
or independently-manufactured capillaries are clamped with the aid of the
appropriate grip head.

12 3 4

18



/

5

3 Mode of operation

Other accessories

Grip head 0,
incl. 2 sets of O-rings and sealing washer.

Using grip head 0 supplied, capillaries with an outer
diameter of 1.0 mm to 1.1 mm can be used. In the
case of outer diameters which differ from these
measurements, the relevant grip heads can be orde­red as non-standard accessories for the universal
capillary holder (see Section 7, Ordering informa­tion). The transparent grip head allows the user to
visually monitor the position of the capillary.

3.4 Information on working practices

The following pressures can be used:

Name Pressure range Comments

Compensation
pressure pc

Injection pressure p

Clean pressure maximum

Constant working
pressure pw

System pressure ps
[supply pressure]

0; 5 – 6,000 hPa

0.00; 0.07 – 87.0 psi

0; 5 – 6,000 hPa

i

0.00; 0.07 – 87.0 psi

up to 7,000 hPa
maximum up to 102 psi

0; 5 – 6,000 hPa

0.00; 0.07 – 87.0 psi

maximum
up to 7,000 hPa
maximum up to 102 psi

Select Function 4 via
the key.

This is used directly for

.

In certain phases of
initialization, the
system pressure may
temporarily increase
to a maximum of
8,000 hPa (117 psi).

19

3 Mode of operation

The usual values for the permanent compensation pressure Pc are bet­ween 30 – 50 hPa (0,44 – 0,73 PSI) for Femtotips and similar capillaries.
The FemtoJet shows good, constant values within this range Immediately
after the device is switched on and maintains the balance between
undesired absorption of medium on the one hand and excessive loss of
the liquid to be injected on the other hand.

Quite low P
(e.g. capillaries with an interior diameter of 5 µm and greater)! In order to
be able to also work precisely and efficiently here, the FemtoJet should be
switched on app. 60 to 90 minutes before beginning the experiment to
guarantee the correct thermal settings. A pressure setting below 5 hPa is
not possible. You may merely also set 0 hPa.
An internal self-calibration of 3 seconds for more precise adjustment of
the pressure takes place every 10 minutes. No injection is carried out
during this time.
Injection can continue as usual following the self-calibration.
An already activated injection will be carried out by the FemtoJet. The
self-calibration takes place afterward.
Message during the self-calibration on the display of the FemtoJet:

values are sometimes advisable for special applications

c

WAIT

self calibration

If the FemtoJet is connected with the InjectMan NI 2, the following
message appears on the display of the micromanipulator:

Warning 001
Check Injector and cable
Press joystick button

Self-calibration intervals:

– In the first hour after the machine is switched on: every 10 minutes.

– Afterwards: Every 30 min at pc < 30 hPa or every 15 min at pc > 30 hPa

following the last activation.

20

A brief signal sounds at the beginning
and end of the calibration.

Press the joystick button again to repeat
the injection.

3 Mode of operation

3.4.1 Compensation pressure pc

The compensation pressure pc ensures that no medium flows into the
capillary. Capillary forces would make liquid flow out of the cell culture
dish into the injection capillary and thus dilute the injection material. To
prevent this, a permanent compensation pressure p
be selected so that there is a permanent slight flow-out of liquid from the
injection capillary. The individual pressure level can be determined in a
preliminary test. For example, a fluorescent dye can be added to the
capillary to enable outflow to be monitored directly. The pressure required
is heavily dependent on the surface tension and viscosity of the injection
material. Experience has shown that compensation pressures of between
30 hPa and 300 hPa (0.44 – 4.4 psi) are appropriate values for ready-to­use Eppendorf Femtotips



injection capillaries.

is set. This should

c

Capillary suction

Compensation pressure p

Hydrostatic pressure P

hyd

c

Pressure in the capillaries

3.4.2 Injection pressure p

i

Injection pressure pi is applied during the injection process. This pressure
is generally higher than the compensation pressure. In many cases, a
pressure between 50 hPa and 500 hPa (0.73 – 7.2 psi) is appropriate for
Femtotips. The volume injected is dependent on the injection pressure
and the injection time. The injection pressure must be greater than
the internal pressure of the cell into which injection should take place.
However, if the cell or the cell nucleus swell up during injection, the
injection pressure is too high.

If the compressor starts up occasionally during experiments, this does not
impair the function of the FemtoJet



. Pressure is always available up to
the maximum value of 6,000 hPa (approx. 87.0 psi), even when the
compressor is topping up the supply pressure.

21

3 Mode of operation

3.4.3 Injection time t

Injection time ti gives the period for which injection pressure pi is
maintained. t

1.5 s. The relevant factor for the injected volume is the product of the
injection pressure and the injection time.

Depending on the setting on the Eppendorf Micromanipulator which is
connected, the injection time ti is measured from the point at which the

is already present during the injection movement and during penetration
of the cell. Once the end of injection time has been reached, the device
switches to compensation pressure pc. At the same time, the micromani­pulator is moved back into the working level. When "Injection at Z limit" is
set, measurement of the injection time ti does not begin until the Z limit
has been reached. Once ti has elapsed, the FemtoJet switches back to
pc and the micromanipulator moves back into its starting position in the
working level.

is usually selected from a range between approx. 0.3 s and

i

key is pressed. The injection pressure is built up immediately, so it

i

3.4.4 Clean function

The Clean function is used to flush out blocked capillaries. The maximum
available pressure is applied to the outlet with the key. It is applied
for as long as the key is pressed. The maximum pressure of the
FemtoJet

changed.
The pressure curve up to maximum pressure appears in the display in the

form of a bar chart. By pressing the key briefly, a lower pressure
can also be obtained.



is in the range of 7,000 hPa (102 psi). The value cannot be

22

3 Mode of operation

3.4.5 Manual or automatic injection

In the Manu operating mode, injection is performed for as long as the

key or hand / foot control is held down. The duration (ti) of

injection is measured and shown in the display. In the case of
combination with an Eppendorf Micromanipulator, the injection movement
is triggered.

In the Auto operating mode, the built-in timer facilitates a reproducible
injection time. The injection runs automatically for the duration of set time

t

. It is triggered by the key or the hand / foot control being pressed

i

briefly. During injection, the Inject key is inactive, i.e. pressing it again has
no effect. In combination with an Eppendorf Micromanipulator, the auto­matic injection procedure can also be triggered from the operating panel
of the micromanipulator.

3.4.6 Constant pressure/constant working pressure

Using the Constant Pressure function, it is possible to select a higher
pressure permanently if required. This function is helpful when special
injection techniques require either a high pressure up to a maximum of
6,000 hPa (87 psi) or a permanent flow of injection solution
(see Section 3.7.5). Control accuracy drops in the pressure range from
2,000 – 6,000 hPa (29 – 87 psi).

3.4.7 General note

FemtoJet is fitted with an integrated compressor. For this reason, the
compressor may start up or excess pressure may be let off briefly before,
during and after injections or during periods when the FemtoJet
being used. This is not a malfunction of the device and does not impair
injection.

In the case of injections under high pressure at very short intervals
or in the case of the Clean function being carried out several times
in succession, the pressure in the system accumulator may drop
significantly. This is indicated by a warning or an error message. In this
case, wait a short time until the supply pressure has stabilized again.
It is then possible to continue working immediately.



is not

23

3 Mode of operation

3.5 The first injection

The following are available:

● Adherent cells in a Petri dish (possibly on CELLocates to observe

specific cells after injection),

● Injection liquid (purified, e.g. by centrifugation),

● Femtotips

● Microloader,

● 0.5 – 10 µl pipette to take the Microloader,

● A universal capillary holder on the micromanipulator which has been

preadjusted with a Femtotip



/ Femtotips II; other capillaries as an alternative



.

Filling the Femtotips



with the Microloader:

– Select function 0 "Capillary exchange" by pressing . Connect

the injection tube.

– Aspirate approximately 0.5 – 5.0 µl of centrifuged injection fluid with

the Microloader without touching the base of the vessel. Using the
Microloader, fill the Femtotip
tip approximately up to the centre of the Femtotips



through the rear opening, inserting the



and releasing the

fluid there.



– Hold the Femtotip

vertically, loosen the cap by turning it and allow it

to drop vertically.



– Screw the Femtotip

into the capillary holder.

– Complete Function 0 by pressing . Compensation pressure is

applied.

– Move the capillary into the cell medium (Petri dish) with the micro-

manipulator.

– Focus the cells with the microscope, position the capillary slightly

above the cells but do not allow it to come into contact with the base
of the Petri dish. Focus the capillary.

– Using the key, check that the capillary is not blocked. Either

the fluid can be seen flowing out or it is rendered visible by the
fluorescent substance it entrains. As a rule, any air bubbles present
are blown out.

– Perform a sample injection using the preset parameters

~ 150 hPa, ti ~ 0.5 s, pc ~ 50 hPa, corresponding to

(e.g. p

i

p

~ 2.18 psi, ti ~ 0.5 s, pc ~ 0.73 psi). Injection can be monitored

i

visually when the cell size changes by approx. 5 % to 10 % or via
fluorescence. If required, adapt parameters p

and/or ti.

i

24

3 Mode of operation

3.6 Connecting an Eppendorf Micromanipulator

Connection to an Eppendorf Micromanipulator designed for automated
injection is effected with the 15-pin interface.

1

2



FemtoJet

1 Foot control (optional) with Inject function
2 Hand control with Inject and Clean functions
3FemtoJet
4 Micromanipulator InjectMan NI 2
5 Connection socket of micromanipulator for Interface cable FemtoJet
6 Interface cable FemtoJet (accessories: InjectMan
7 Interface of FemtoJet

(rear) with all connection options, with InjectMan NI 2



3



to micromanipulator

67

45



NI 2)

25

3 Mode of operation

2

1

345

8

67

FemtoJet (rear) with all connection options, with power unit 5171/
InjectMan 5179

1 Foot control (optional) with Inject function
2 Hand control with Inject and Clean functions
3FemtoJet
4 Micromanipulator 5171 or InjectMan





5 Connection socket of micromanipulator for

Transmission cable 5246 ("Y cable")

6 Transmission cable 5246

(accessory for Micromanipulator 5171 and InjectMan

7 Interface cable 5171 (optional)

(Connection from FemtoJet

8 Interface of FemtoJet





to Transmission cable 5246 "Y cable")

to micromanipulator



)

3.7 Functions

The Function menu is called up using the key. One of seven
functions is selected using left-hand variable regulator p
functionality is assigned to the variable regulators in the bottom line of the
display.

Exit the Function menu by pressing the key again.

In cases of error, pressure control is switched off from within the Function
menu. Then exit the Menu using the key.

Function 6, self-test, is described in Section 5, "Troubleshooting".

26

. The relevant

i

3 Mode of operation

3.7.1 Changing the capillaries

FUNC 0
Change capillary

Capillary may be

changed now
Tu r n pi to
select function

FUNC 0

When this function is called up, the
injection tube is deaired and any
pressure present in the system is let out.
Once the capillary has been changed,

exit the function using the key or
variable regulator p

restart.

3.7.2 Setting the pressure unit

FUNC 1
Push Inject to select
Pressure Unit hPa PSI

Tu r n pi to
select function

FUNC 1

The other pressure unit is selected using
the key and all pressure values
are converted. The pressure unit

currently selected (in this case: hPa)
is underlined.

3.7.3 Switching on/off the loudspeaker

FUNC 2
Push Inject to select
Beeper ON

Tu r n pi to
select function

OFF

FUNC 2

Use the key to switch the
loudspeaker on or off. The currently
selected setting (in this case: ON) is
underlined. Acoustic error messages are
not emitted if the setting
is OFF.

; pressure control will

i

3.7.4 Switching on/off the background illumination of the display

FUNC 3
Push Inject to select
Illumination ON OFF

Tu r n pi to
select function

FUNC 3

Use the key to switch the
illumination on or off. The currently
selected setting (in this case: ON) is
underlined.

In the normal operating mode,
illumination switches off after three
minutes if no operating control has been
actuated. When an operating control is
actuated, the illumination lights up again.

27

3 Mode of operation

3.7.5 Constant pressure

FUNC 4
Continuous flow
INJECT = start

Tu r n pi to
select function

FUNC 4
Continuous flow

pw= 50 hPa

Tu r n pi to pw[hPa]
select function 50

FUNC 4

This function can be used to apply a
constant working pressure p
range from 5 to 6,000 hPa (87.0 psi) at
the pressure outlet. The pressure unit psi
can also be used.

The working pressure p
the right-hand variable regulator (p
the specified value is shown in large
characters. The actual value is shown
in the bottom right of the display.

The Clean function can be activated. The
pressure of the Clean function is shown
in the bottom right of the display in the
form of p

.

w

3.7.6 Dewatering the pressure accumulator

FUNC 5
Drain pressure supply
INJECT = start

Tu r n pi to
select function

FUNC 5

– Briefly opens the dewatering valve

of the pressure accumulator; any
condensed humidity present is blown
away.

– When this function is performed, the

noise of air being blown away can be
heard.

This function is automatically performed
every time the unit is switched on. It can
be performed manually as described if
condensation is suspected to be present.

in the

w

is set with

w

),

c

28

4 Cleaning and Service

4.1 Cleaning

Before cleaning the FemtoJet, disconnect the plug.

Ensure that no fluids enter into the inside of the injector in order to avoid
short-circuits in the electrical installation as well as corrosion.

Wipe painted parts and aluminum surfaces using a cloth and mild
detergent and then with a dry cloth.

Warning:

or polishes.

4.2 Disinfection

Clean the FemtoJet before disinfecting.

Wipe every part of the FemtoJet, including accessories and
connector cables, with disinfectant like alcohol (ethanol, isopropanol) or
alcoholic disinfection media.

Spray disinfection is not advisable, as disinfectants can enter into the
microinjector.

The disinfection method used must comply with current disinfection
regulations and guidelines.

Do not use any corrosive, solvent or abrasive detergents

4.3 Decontamination prior to shipping

If the device is to be inspected, repaired or calibrated by the

service partners of the Eppendorf AG, it must be free of
hazardous substances and clean!

A decontamination certificate must accompany the FemtoJet.

A printed form "Decontamination certificate for return of goods" and
general instructions for decontamination are available at our
www.eppendorf.com homepage.

The serial number of the device must be entered in the decontamination
certificate.

4.4 Service

Warranty or service is taken over by the supplier or the service partner of
the Eppendorf AG.

29

5 Troubleshooting

5 Troubleshooting

5.1 Potential errors

– Pressure loss due to capillary not fitted with capillary holder

connected.
Solution: Connect the capillary or select Function 0, Change capillary,

– Capillary is blocked.

Solution: Press the key for long enough.

– Compressor runs for a long time.

Solution: Take a short break.

–Valve rattles

Solution: Connect tube to filled capillary. Pressure control is designed

– No communication with the micromanipulator.

Solution: Check cables and connections.

via the key and variable regulator p

Change capillary.
Purify injection fluid (e.g. by means of centrifugation).

Do not use the key too often within a short period.
If possible, set a lower compensation pressure p

Perform self-test (Section 5.1.1).

for the tube connected.

.

i

c.

30

5.1.1 Self-test

FUNC 6
Self-Test
INJECT = Complete
CLEAN = Leak only

Tu r n pi to
select function

FUNC 6

The FemtoJet is checked for leaks in
the self-test.

This self-test can also be used if a leak is
suspected.

The test runs automatically.

The injection tube must be connected to
a closed capillary (e.g. melted closed or
filled) for the pressure control to work
properly.

5 Troubleshooting

FUNC 6
Self-Test
Compressor _____ s
Ps leak _____ _____ hPa
Pw leak _____ _____ hPa
Injection _____ _____ hPa

ps=7000 pw= 18 hPa

FUNC 6
Self-Test

Ps leak _____ _____ hPa
Pw leak _____ _____ hPa

ps=7000 pw= 18 hPa

key: Complete self-test

– Checks compressor run time up to

the point when a specific pressure
is reached and thus for leaks in the
area of the compressor.

– In the case of two different

pressures, checks for leaks in the
pressure accumulator (p
the internal tightness of the valves.

) and for

s

– Checks for leaks in the area of

working pressure (pw) in the case
of two different pressures.

– Checks behavior during injection

at different pressures (short
continuous test).

If certain values are outside the specified
range, an error message is emitted. If this
recurs, inform Service.

key: Self-test to search for leaks

– Checks for leaks in the pressure

accumulator (p
different pressures, as well as for

) in the case of two

s

inner tightness of the valves.

– Checks for leaks in the area of wor-

king pressure (pw) in the case of two
different pressures.

If certain values are outside the specified
range, an error message is emitted. If this
recurs, inform Service.

31

5 Troubleshooting

5.2 Warnings

As a rule, warnings are emitted if certain operating conditions are not (yet)
reached or if an action is not possible.

Acknowledge the warning with the key and the unit will return to
normal status.

WARNING 001

Please wait for

supply pressure

INJECT = Continue

WARNING 002

Check capillary

e.g. broken/missing ?

INJECT = Continue

Warning 1

Supply pressure has not yet been
reached.

The minimum supply pressure required
for an injection or the Clean function was
not present when the function was
triggered.

Possible cause:
Multiple triggering of Clean or very rapid
injection at a high injection pressure.

Solution:
Wait until the supply pressure has built up
and trigger again.

Warning 2

Compensation pressure cannot be
maintained.

Possible cause:
Capillary or capillary holder not attached
or only connected loosely.

Solution:
Check all connections and tighten.
Activate Function 0 to change the
capillary and when the capillary holder is
open.

32

5 Troubleshooting

WARNING 003

Switch on manipulator

or disconnect cable

INJECT = Continue

Warning 3

Micromanipulator does not react

A micromanipulator is connected but
does not react to a start pulse from the
FemtoJet.

Possible cause:
Micromanipulator is switched off.
INJECT function on manipulator is not
released.
No limit is set on the micromanipulator.
In the INJECT function on the micro­manipulator, synchronization is set to
limit (SYNC = LIMIT).

Solution:
Switch on the micromanipulator and
make it operational.
Disconnect the cable to micro­manipulator.
With SYNC = LIMIT, trigger semi-auto­matic injection via the micromanipulator
only.

5.3 Error messages

Error messages are emitted if certain operating conditions are not
reached or the device records a deviation from specified conditions.

Acknowledge the error message with the key and the unit will
return to normal status. The measures described under “Solution” should
be carried out. If the error message recurs after acknowledgement with
the key, inform Service.

33

5 Troubleshooting

ERROR 010

Supply pressure

cannot be reached

INJECT = Continue

ERROR 011

Supply pressure is

too high

INJECT = Continue

Error 10

Supply pressure cannot be reached

The compressor runs without the
maximum supply pressure being
reached.

Possible cause:
High compensation pressure when the
capillary holder is not attached or is
poorly attached.

Technical cause:
Compressor does not start up. Leak in
compressor or pressure accumulator.

Solution:
Check the capillary holder and tube for
leaks or tighten up the tube connections.
Service.

Error 11

Supply pressure is too high

Supply pressure has exceeded the
maximum permissible supply pressure.

Possible cause:
supply pressure is not relieved by the
relevant standby function.
Technical defect.

Solution:
Switch off using the key (hold
down for approximately two seconds).
Service.

34

ERROR 012

Supply pressure is

does not fall

INJECT = Continue

Error 12

Supply pressure does not drop far
enough

Possible cause: Technical defect

Solution: Service

5 Troubleshooting

ERROR 013

Supply pressure is

does not raise

INJECT = Continue

ERROR 014

Supply pressure

leak is too high

INJECT = Continue

ERROR 015

Supply pressure

leak is too high

INJECT = Continue

ERROR 016

Valve 1 pressure

leak is too high

INJECT = Continue

ERROR 020

Working pressure

cannot be reached

INJECT = Continue

Error 13

Supply pressure does not rise

Possible cause: Technical defect

Solution: Service

Error 14

Massive leak in pressure accumulator

Possible cause: Technical defect

Solution: Service

Error 15

Leak in pressure accumulator

Possible cause: Technical defect

Solution: Service

Error 16

Leak through Valve V1 is too large

Possible cause: Technical defect

Solution: Service

Error 20

Working pressure has not been reached

Compressor runs without supply
pressure being reached within a specific
time.

Possible cause:
Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.

Solution:
Check the capillary. Check the capillary
holder and tube for leaks or tighten up the
tube connections.
Service.

35

5 Troubleshooting

ERROR 021

Inject pressure

cannot be reached

INJECT = Continue

ERROR 022

Compensation pressure

cannot be reached

INJECT = Continue

Error 21

Injection pressure has not been reached

Possible cause:
Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.

Solution:
Check capillary. Check capillary holder
and tube for leaks or tighten up tube
connections.
Service.

Error 22

Compensation pressure has not been
reached

Possible cause:
Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.

Solution:
Check capillary. Check capillary holder
and tube for leaks or tighten up tube
connections.
Service.

36

ERROR 023

Inject n pressure

overflow too large

INJECT = Continue

ERROR 024

Working pressure

does not rise

INJECT = Continue

Error 23

Increase in working pressure is smaller
than required

Possible cause: Technical defect

Solution: Service

Error 24

No increase in working pressure

Possible cause: Technical defect

Solution: Service

5 Troubleshooting

ERROR 025

Working pressure

does not fall

INJECT = Continue

ERROR 026

Working pressure

leak is too high

INJECT = Continue

ERROR 027

Working pressure

leak is too high

INJECT = Continue

Error 25

No drop in working pressure

Possible cause: Technical defect

Solution: Service

Error 26

Working pressure leak rate is too high

Possible cause:
Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.

Solution:
Check capillary. Check capillary holder
and tube for leaks or tighten up tube
connections.
Service.

Error 27

Working pressure leak rate is too high

Possible cause:
Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.

Solution:
Check capillary. Check capillary holder
and tube for leaks or tighten up tube
connections.
Service.

37

5 Troubleshooting

ERROR 028

Working pressure

leak is too high

INJECT = Continue

ERROR 030

Sensoroffset supply

is too low

INJECT = Continue

ERROR 031

Sensoroffset supply

is too high

INJECT = Continue

ERROR 032

Sensoroffset work

is too low

INJECT = Continue

Error 28

Working pressure leak rate is too high

Possible cause:
Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.

Solution:
Check capillary. Check capillary holder
and tube for leaks or tighten up tube
connections.
Service.

Error 30

Sensor offset supply pressure is too low

Possible cause: Technical defect

Solution: Service

Error 31

Sensor offset supply pressure is too high

Possible cause: Technical defect

Solution: Service

Error 32

Sensor offset working pressure is too low

Possible cause: Technical defect

Solution: Service

38

ERROR 033

Sensoroffset work

is too high

INJECT = Continue

Error 33

Sensor offset working pressure is too
high

Possible cause: Technical defect

Solution: Service

5 Troubleshooting

ERROR 034

Val ve 1 offset

is too low

INJECT = Continue

ERROR 035

Val ve 1 offset

is too high

INJECT = Continue

ERROR 036

Val ve 2 offset

is too low

INJECT = Continue

ERROR 037

Val ve 2 offset

is too high

INJECT = Continue

ERROR 040

Self-Calibration

is missing

INJECT = Continue

Error 34

Valve 1 offset is too low

Possible cause: Technical defect

Solution: Service

Error 35

Valve 1 offset is too high

Possible cause: Technical defect

Solution: Service

Error 36

Valve 2 offset is too low

Possible cause: Technical defect

Solution: Service

Error 37

Valve 2 offset is too high

Possible cause: Technical defect

Solution: Service

Error 40

Self-calibration is missing

Possible cause: Technical defect

Solution: Service

ERROR 041

Fullscale Calibration

is missing

INJECT = Continue

Error 41

Full-scale calibration is missing

Possible cause: Technical defect

Solution: Service

39

6 Technical data

Voltage/Frequency: 100 V – 240 V ± 10 %, 50 – 60 Hz
Set voltage: adapts automatically
Fuse for 100 – 240 V: T1.0 A, 250 V (2 pcs.)
Power consumption: 50 W
Max. current consumption: < 0.5 A
Protection class:
Overvoltage category:
Pollution degree: 2 (IEC 664)
Ambient temperature: 15 °C – 35 °C
Ambient relative humidity: max. 70 %
Weight: 6.2 kg
Dimensions: Width: 220 mm

Injection time t

Injection pressure p

Compensation pressure p

Regulating accuracy: 1 % of value set in each case,

Clean rinsing pressure: max. approx. 7,000 hPa (102 psi)
RS 232 computer Baud rate: 9,600

interface: Start bit: 1

: 0.0 – 99.9 s

i

:0; 5 – 6,000 hPa (87 psi),

i

I

II

(IEC 61010-1)

Depth: 170 mm
Height: 280 mm

in 0.1 s increments

in 1 hPa (0.01 – 0.02 psi) increments

: 0; 5 – 6,000 hPa (87 psi),

c

in 1 hPa (0.01 – 0.02 psi) increments

at least ± 1 hPa

Data bits: 8
Parity: non
Stop bit: 2

The device is approved with the sign.

U.S. Pat.No. 4,836,244

Technical specifications subject to change!

40

7 Ordering information

7 Ordering information

Order no.

5247 000.013 FemtoJet



100 V – 240 V ± 10 %, 50 – 60 Hz
Fully programmable microinjector with integrated
pressure supply. For injection of smallest amounts
of liquid into living cells.
Delivery package:
1 FemtoJet



1 Power cable
1 Universal capillary holder
1 Injection tube, 2 m

5247 617.002 1 Hand control for remote control

10 Femtotips



/ Femtotips II (injection capillaries)
10 Microloader
1 Operating Manual

5247 000.013 Version Europe
5247 000.021 Version USA and Canada
5247 000.030 Version UK/Hongkong
5247 000.048 Version Japan
5247 000.056 Version South America
5247 000.072 Version Australia
5247 000.080 Version China

Accessories (not standard)

5247 623.002 Foot control FemtoJet



5325 620.007 Interface cable PC

for control and for program updates

5181 070.015 Interface cable FemtoJet



for connection to the

InjectMan NI 2

5247 622.006 Interface cable 5171

for connection to the micromanipulator 5171 or
InjectMan

5242 952.008 20 Femtotips



5179



5242 957.000 20 Femtotips II
5242 009.012 Filling stand for Femtotips



5242 956.003 200 Microloader
5246 164.004 1 Injection tube, 2 m, for universal capillary holder

41

7 Ordering information

Order no.

5176 190.002 Universal capillary holder

(incl. grip head 0, adapter for Femtotips



and spare O-rings)
4910 000.018 Eppendorf Reference
3111 000.122 Eppendorf Research



pipette, variable, 0.5 to 10 µl



pipette, variable, 0.5 to 10 µl

5176 210.003 Grip head 0 for universal capillary holder;

for microcapillaries with an outer diameter

of 1.0 to 1.1 mm
5176 212.006 Grip head 1 for universal capillary holder;

for microcapillaries with an outer diameter

of 1.2 to 1.3 mm
5176 214.009 Grip head 2 for universal capillary holder;

for microcapillaries with an outer diameter

of 1.4 to 1.5 mm
5176 207.002 Grip head 3 for universal capillary holder

for microcapillaries with an outer diameter

of 0.7 to 0.9 mm
5176 196.000 O-ring set

for grip heads 0 to 3 with extraction tool
5176 195.004 Service kit

for grip head and capillary holder,

consisting of O-ring set, adapter for Femtotip

and clamping piece (for 5171, 5179)

Micromanipulation

InjectMan NI 2

Micromanipulator for automatic injection in adherent

cells in combination with FemtoJet



5181 000.017 Version Europe
5181 000.025 Version USA and Canada
5181 000.033 Version UK/Hongkong
5181 000.041 Version Japan
5181 000.076 Version Australia
5181 000.084 Version China



42

7 Ordering information

Order no.

Tr ansferMan NK 2
Proportional micromanipulator for microinjection

in suspension cells
5188 000.012 Version Europe
5188 000.020 Version USA and Canada
5188 000.039 Version UK/Hongkong
5188 000.047 Version Japan
5188 000.071 Version Australia
5188 000.080 Version China

PatchMan NP 2

Micromanipulator for electrophysiology or other

applications
5183 000.014 Version Europe
5183 000.022 Version USA and Canada
5183 000.030 Version UK/Hongkong
5183 000.049 Version Japan
5183 000.073 Version Australia
5183 000.081 Version China

5246 621.006 Transmission cable 5246

"Y cable" for connection with the

interface cable 5171
5211 058.007 Foot control for microinjection,

connectable to transmission cable 5246 "Y cable"

43

7a Ordering information for North America

Order no.

FemtoJet



100 V – 240 V ± 10 %, 50 – 60 Hz
Fully programmable microinjector with integrated
pressure supply. For injection of smallest amounts
of liquid into living cells.
Delivery package:
1 FemtoJet



1 Power cable
1 Universal capillary holder
1 Injection tube, 2 m

920010512 1 Hand control for remote control

10 Femtotips



/ Femtotips II (injection capillaries)
10 Microloader
1 Operating Manual

920010504 Version USA and Canada
5247 000.013 Version Europe
5247 000.030 Version UK
5247 000.048 Version Japan
5247 000.056 Version South America
5247 000.072 Version Australia
5247 000.080 Version China

Accessories (not standard)

920005098 Foot control FemtoJet



015160501 Interface cable PC

for control and for program updates

920005845 Interface cable FemtoJet



for connection to the

InjectMan NI 2

5247 622.006 Interface cable 5171

for connection to the micromanipulator 5171 or
InjectMan

930000035 20 Femtotips



5179



930000043 20 Femtotips II
930000051 Filling stand for Femtotips



930001007 200 Microloader
920007431 1 Injection tube, 2 m, for universal capillary holder

44

7a Ordering information for North America

Order no.

920007392 Universal capillary holder

920007414 Grip head 0 for universal capillary holder;

910012509 Grip head 1 for universal capillary holder;

910012517 Grip head 2 for universal capillary holder;

920007406 Grip head 3 for universal capillary holder

920005870 O-ring set

920005888 Service kit

(incl. grip head 0, adapter for Femtotips
and spare O-rings)

for microcapillaries with an outer diameter
of 1.0 to 1.1 mm

for microcapillaries with an outer diameter
of 1.2 to 1.3 mm

for microcapillaries with an outer diameter
of 1.4 to 1.5 mm

for microcapillaries with an outer diameter
of 0.7 to 0.9 mm

for grip heads 0 to 3 with extraction tool

for grip head and capillary holder,
consisting of O-ring set, adapter for Femtotip
and clamping piece (for 5171, 5179)



Micromanipulation

InjectMan NI 2
Micromanipulator for automatic injection in adherent

cells in combination with FemtoJet
920000029 Version USA and Canada
5181 000.017 Version Europe
5181 000.033 Version UK/Hongkong
5181 000.041 Version Japan
5181 000.076 Version Australia
5181 000.084 Version China





45

7a Ordering information for North America

Order no.

Tr ansferMan NK 2
Proportional micromanipulator for microinjection

in suspension cells
920000011 Version Europe
5188 000.020 Version USA and Canada
5188 000.039 Version UK/Hongkong
5188 000.047 Version Japan
5188 000.071 Version Australia
5188 000.080 Version China

PatchMan NP 2

Micromanipulator for electrophysiology or other

applications
920000037 Version USA and Canada
5183 000.014 Version Europe
5183 000.030 Version UK/Hongkong
5183 000.049 Version Japan
5183 000.073 Version Australia
5183 000.081 Version China

920012043 Transmission cable 5246

920005047 Foot control for microinjection,

46

"Y cable" for connection with the

interface cable 5171

connectable to transmission cable 5246 "Y cable"

8

Index

A

Adapter for Femtotips 18
Application 3, 4

C

Capillary change 27
Clean 8

pressure 20

function 22
Cleaning 29
Commands 46
Compressor 21, 23
Compensation pressure pc 5, 20,

21
Connection

capillary holder 18

micromanipulator 25

universal capillary holder 18
Constant

pressure 23, 27

working pressure 23

working pressure p

20

w

D

Disinfection 29
Display 6, 8, 12

in hPa 12

in psi 12

switch on/off 27

E

Error

messages 33

potential 30

F

Foot control 7, 17
Functions 26

keys 8

menu 15

G

Grip head 18, 19

H

Hand control 17

I

Information on working practices

20

Injection

automatic 14, 23
first 24
manual 14, 23
movement 14, 23
parameters 12
pressure pi 5, 14, 20, 21
time t

5, 14, 22

i

trial 24
tube 9, 18

Interface 10, 25

description 45

K

Key

Auto/Manu 8, 14
Clean 8, 14, 22
Count 8, 15
Inject 5, 8, 14, 32
Menu 8, 26
Standby 8, 16

Keypad 6, 14

L

Loudspeaker, switch on/off 27

M

Mains switch 10
Menu 15
Mode of operation 12

O

Operation 12

via computer 44, 46
Ordering information 41
Overview 6

47

8 Index

P

Pressure

– dewatering
pressure accumulator 28
– set pressure unit 26

Q

Query 45

R

Reset 9, 17

function 16

S

Safety precautions 3, 4
Self-test 30

complete 31

to find leaks 31
Servicing 29
Short description 8
Startup 10
Standby without deairing 16
Switch

on 10

off 11
System pressure ps 20

T

Technical data 40
Tension piece 19
Troubleshooting 30
Tube connection 6, 9

U

Unit

description 5

rear of 10
Universal capillary holder 18

V

Variable regulator 5, 6, 9, 13, 26

W

Warnings 32

48

Appendix A

Operation via a computer

Certain commands and queries (Section 6.2) can be transmitted to the

unit using the optional cable/PC interface.

A.1 Description of interface

A computer can be connected to the remote control of the FemtoJet via

one of the two RS 232 interfaces. Parameters can be entered or read out

via the computer.

The computer is connected by the special cable/PC interface cable.

The computer to be connected must comply with EN 60950/UL 1950

standards.

The serial interface of the computer should be set to the following

transmission parameters:

Baud rate: 9,600

Start bit: 1

Dat bits: 8

Parity: non

Stop bit: 2

Only ASCII symbols (incl. CR/LF) which can be represented are

transmitted.Therefore a terminal program, for example, is suitable for

remote control.

A.2 Commands and queries

The commands to be sent are defined with the following structure:

Commands to enter parameters: "Cxxx=ssssssssss"

Commands to execute and to read out parameters: "Cxxx"

C: Command

xxx: Code for the executing command

=: Separation for the following input parameters

ssssssssss: Input parameters

In response to any command, the device provides a symbol string of a

similar structure:

"Axxx" for answers without output parameter

"Axxx=rrrrrrrrrr" for responses with output parameter

A: Answer

49

Appendix A

If the command is faulty, an error message is returned instead of the
answer:

"ERR=eee"
ERR: Error
=: Separation
eee: Error number

Lower and upper case letters can be used.

Command to enter
parameters

Short acoustic signal 003 1 – 254 Number of signals

Long acoustic signal 004 1 – 254 Number of signals

Automatic injection
(inject at t

Set injection counter n
to 0

Perform Clean 013 1 – 100 Time in 1/10 seconds

Execute Standby 014 0 or 1 0 = short

Switch acoustic signal
on/off

Set pressure unit 041 0 or 1 Unit:

Set injection time ti 042 1 – 999 Injection time

)

i

Code

Input
parameter

[C]

[xxx]

[ssssssssss]

010 0; 1 – 999 Injection time

012 – – – No parameter input

040 0 or 1 Status:

Meaning of
input parameters

in 1/10 seconds
0: injection time set on
the FemtoJet

1 = long

0 = off / 1 = on

0 = hPa / 1 = psi

in 1/10 seconds



50

Appendix A

Command to enter
parameters

Set compensation
pressure pc

Set injection pressure pi 044 0; 5 – 6,000 /

Set background
illumination

Code

Input
parameter

[C]

[xxx]

[ssssssssss]

043 0; 5 – 6,000 /

87

87

045 0 or 1 0 = off

Meaning of
input parameter

Pressure in hPa/psi

Pressure in hPa/psi

1 = on

Dewater 046 – – – No parameter input

Command to read out
parameters

[C]

Code

Significance of
output parameter

[xxx]

[A]

Output
parameter
[rrrrrrrrrr]

Query program version 101 Versions number x.xx

Query bleeper 150 Status:

0 or 1

0 = off / 1 = on

Query pressure unit 151 Unit:

0 or 1

0 = hPa / 1 = psi

Query injection time t

152 Injection time

i

in 1/10 seconds

1 – 999

Query compensation
pressure p

c

Query injection
pressure p

i

Query background
illumination

153 Pressure in hPa/psi 0 – 6,000

154 Pressure in hPa/psi 0 – 6,000

155 Status:

0 or 1

0 = off / 1 = on

/ 87

/ 87

51

Appendix A

Error no.
for incorrect
operating
construction
ERR = [eee]

060 The first character is not

061 The 2nd to 4th character

062 The 5th character is not =. Check entry parameters.

063 The parameter is not

064 The command is not

Cause Solution

a c or C.

is not a digit.

within the permitted range.

known.

Check entry parameters.
Change entry.

Check entry parameters.
Change entry.

Change entry.

Check entry parameters.
Change entry.

Check entry parameters.
Change entry.

52

Appendix A

Error no.

Cause Solution
during
execution ERR
= [eee]

070 FemtoJet is not in routine

mode.

Check FemtoJet.
Bring FemtoJet



into
routine operation.
Eliminate error.

071 FemtoJet



is not ready. Check FemtoJet or

micromanipulator.
As necessary, wait or
eliminate error.

072 The command is not finished

within the scheduled time.

Check FemtoJet
micromanipulator.



or

Eliminate error.

073 This command cannot be

executed at the moment,

Check FemtoJet
Eliminate error.



.

because of insufficient storage
pressure.
See: Warning 001

074 The connected micro-

manipulator did not respond.
See: Warning 003

Check micro­manipulator.
Eliminate error.

Other error numbers as well as the error cause and solution are described
in Section 5, "Troubleshooting". During remote control, no error texts
appear in the FemtoJet



display.

53

Appendix A

54