Instructions for use
—
epT.I.P.S.®seeon p.Fig.Tab.p
Inst
.
ructions for use
0012 580.479
epT.I.P.S.®
Instructions for use
Version B
Copyright© 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.
®
, Eppendorf®, Eppendorf Research®, Eppendorf Reference®, Eppendorf Xplorer®,
Biopur
epT.I.P.S.® and ep Dualfilter T.I.P.S.® are registered trademarks of Eppendorf AG, Hamburg,
Germany.
Registered trademarks are not marked in all cases with ™ or ® in this manual.
0012 580.479-01/102012
Version B
epT.I.P.S.® — Instructions for use
Table of contents
1 Table of contents
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2 Instructions for use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
3 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
5 Handling reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
6 ep Dualfilter T.I.P.S. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
7 epT.I.P.S. LoRetention. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
8 Autoclave pipet tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
9 Purity grades and certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
9.1 Eppendorf purity grades . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
9.2 Certificates - verifiable safety for you . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
10 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
10.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
11 epT.I.P.S. combinations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
12 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
12.1 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
13 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
13.1 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3
epT.I.P.S.® — Instructions for use
1 Introduction
1 Introduction
epT.I.P.S. pipette tips are disposable plastic tips made of polypropylene (PP). epT.I.P.S. stands
for Eppendorf Totally Integrated Pipetting System which means that form, material properties and
fit are optimized for Eppendorf’s manual and electronic piston-stroke pipettes.
The tips are available in a variety of finishes and purity grades. Every tray for epT.I.P.S. pipette
tips is color-coded and corresponds to the color of the control buttons of Eppendorf pipettes.
Beside epT.I.P.S. pipette tips we offer epDualfilterT.I.P.S. pipette tips to minimize the risk of
contamination, epT.I.P.S. LoRetention pipette tips to dispense liquids containing detergents and
epDualfilterT.I.P.S.LoRetention pipette tips which combines both.
2 Instructions for use
2 Instructions for use
• The pipette tips epT.I.P.S., epDualfilterT.I.P.S., epT.I.P.S.LoRetention and
epDualfilterT.I.P.S.LoRetention and relevant pipettes must be used by trained laboratory
workers.
• Before using tips familiarize yourself with the operating manual of the relevant instrument.
• Before using the tips, check pack and contents carefully for any damage. For filter tips, check
whether there is a dualfilter in every tip and whether it is correctly positioned, undamaged
and not twisted. If you detect any fault, do not use the tip.
• The tips should always be emptied into a container so that no fluid sprays into the
environment. After use, dispose of tips in accordance with the relevant legal regulations.
• Tips should not be filled with fluids which attack PP, or in the case of filter tips also PE.
• If you are in any doubt as to the suitability of your product for its intended use or as to the
reagent, please contact our hotline. You will find phone number and address on the last
page.
3 Intended use
3 Intended use
epT.I.P.S., in combination with adequate pipettes, are intended for dispensing liquids in the
volume range of 0.1L–10mL. In vivo applications (applications in or on the human body) are
not allowed. epT.I.P.S. and the appropriate pipettes only must be used by correspondingly trained
specialists. The user must have read thoroughly the operating instructions of the pipette and the
instructions for use of the epT.I.P.S. and must have made himself familiar with the functioning of
the pipette with the pipette tips.
4
epT.I.P.S.® — Instructions for use
4 Operation
4 Operation
You will find recommendations for handling in the operating manual for the instrument you are
using.
The dispensing techniques used most frequently are forward pipetting, reverse pipetting,
dispensing, sequential dispensing and diluting. Mechanical pipettes are only suitable for forward
and reverse pipetting.
Irrespective of the dispensing technique use, the following items should be taken into
consideration during pipetting:
• When aspirating the liquid, the tip should only be immersed a few millimeters into the
medium.
• The filled tip should be moved up against the wall of the vessel to avoid residues of liquid on
the outside of the tip.
• Pre-wetting the tip up to three times will improve the accuracy and precision of the results.
• Liquid should be aspirated slowly and evenly.
• A waiting period of 3 seconds should be allowed for the liquid to rise in the tip.
• The pipette should be held vertically during aspiration.
5
epT.I.P.S.® — Instructions for use
5 Handling reloads
5 Handling reloads
"clic"
6
epT.I.P.S.® — Instructions for use
6 ep Dualfilter T.I.P.S.
6 ep Dualfilter T.I.P.S.
During pipetting liquid flows through a small opening. A possible outcome of this are aerosols
(dispersion of solid or liquid particles in gas). Aerosols, drops and splashes could contaminate
the pipette and contaminations could be transferred to other samples.
To avoid these unintended and unnoticed contaminations epDualfilterT.I.P.S. pipette tips should
be used. The dualfilter is made up of hydrophobic polyethylene(PE). The first white layer defends
from splashes and drops and the second blue layer builds an aerosol barrier.
epDualfilter T.I.P.S. pipette tips are sterilized by electron beams and are certified
(see Certificates - verifiable safety for you on p. 9). epDualfilter T.I.P.S. pipette tips can not be
sterilized.
7 epT.I.P.S. LoRetention
7 epT.I.P.S. LoRetention
epT.I.P.S. LoRetention pipette tips are particularly suited to the dispensing of liquids containing
detergents. The optimized surface of the epT.I.P.S. LoRetention pipette tips counteracts the
reduced surface tension caused by detergents and also with corresponding solvents, so that the
liquid runs off without forming a film. This increases sample recovery and pipetting accuracy.
Please note:
The small amounts of residual moisture that may occur with epT.I.P.S. LoRetention pipette tips
can appear to be higher than with standard epT.I.P.S. pipette tips as the residual moisture does
not remain in the tip as a film, but collects as droplets due to the physical properties.
Due to the physical properties of the surface of epT.I.P.S. LoRetention pipette tips, the dispensing
volume is slightly lower than the nominal volume in comparison to the corresponding standard
epT.I.P.S. pipette tips when dispensing purely aqueous solutions with epT.I.P.S. LoRetention
pipette tips 0.1–10LS and 0.5–20LL. Reproducibility still has the same high performance
as standard epT.I.P.S. pipette tips.
8 Autoclave pipet tips
8 Autoclave pipet tips
Don not use any additional desinfectants during autoclaving for box and rack.
Hint!
Product Temperature Pressure Time Number of
ep T.I.P.S. and ep T.I.P.S.
121°C 1 bar 20 min 5 times
LoRetention
ep T.I.P.S. Rack 121°C 1 bar 20 min 10 times
ep T.I.P.S. Box 121°C 1 bar 20 min 100 times
ep Dualfilter T.I.P.S. and ep
Can not be autoclaved.
Dualfilter T.I.P.S.
LoRetention
1. Remove the packaging foil.
2. Autoclave the pipet tips.
possible
cycles
7
epT.I.P.S.® — Instructions for use
9 Purity grades and certificates
9 Purity grades and certificates
9.1 Eppendorf purity grades
Sterile+PCR
clean
Eppendorf
Quality
Relevant quality criteria
Function, tightness,
precision
Low wettability
High chemical
resistance
Sterile PCR clean
Biopur
High thermal stability
High transparency
Precisely molded
Batch testing (certified) for the following purity criteria
Human DNA-free
DNA-free (free of
human + bacterial
DNA)
DNase-free
RNase-free
PCR inhibitor-free
ATP-free
Pyrogen-free
(endotoxin-free)
8
epT.I.P.S.® — Instructions for use
Eppendorf
Quality
Sterile PCR clean
Sterile+PCR
clean
Biopur
Sterile (European
Pharmacopoeia/USP)
epT.I.P.S.
epT.I.P.S.® Standard +
Box + Set
epT.I.P.S.
epT.I.P.S.
epT.I.P.S.
ep Dualfilter T.I.P.S.
epT.I.P.S.®
LoRetention
ep Dualfilter T.I.P.S.
LoRetention
®
®
Reloads
®
Racks
®
Singles
®
®
9.2 Certificates - verifiable safety for you
The number of standards, guidelines and specifications that apply to biological, diagnostic and
industrial laboratories is constantly on the rise. As a result, the need to provide proof of observing
these requirements has also increased.
Three certificate categories are available for Eppendorf liquid handling consumables:
1. Batch-specific certificates are prepared by externally commissioned and recognized
laboratories for the following purity grades:
– Biopur
– PCR clean
– PCR cleanandSterile
You can access the batch-specific certificate at www.eppendorf.com/consumables/
certificates after entering the batch number of your product.
9
epT.I.P.S.® — Instructions for use
2. General certificates of conformity such as:
– Certificate of conformity for products in the PCR clean purity grade
– Certificate of conformity for verifying metal trace concentrations
– Certificate of conformity on the absence of surface active additives
– Certificate of conformity for HEPA classification of ep Dualfilter T.I.P.S. filter tips
3. Quality management and ISO certificates which verify conformity to ISO
All certificates can be downloaded from our homepage www.eppendorf.com/certificates.
10 Troubleshooting
10 Troubleshooting
10.1 General errors
Symptom/
message
Drops form on the
inner wall of the
epT.I.P.S. pipette
tip.
Liquid is dripping
from the tip and/or
the dispensed
volume is incorrect.
Other possible reasons for faults associated with overall operation are described in the operating
instructions for the device being used.
These brief instructions for use do not replace the operating instructions for the individual
devices.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf
CAUTION!
may impair the safety, function and precision of the device. Eppendorf cannot be held
liable or accept any liability for damage resulting from the use of incorrect or
non-recommended accessories and spare parts or from the improper use of such
equipment.
Cause Remedy
Inner wall has not been uniformly
prewet.
The tip is loose or the pipette tip is
poorly fitted.
Pipetted too quickly. Move the control button slowly.
The tip is withdrawn from the liquid
too quickly.
Use new epT.I.P.S. pipette tip
or epT.I.P.S. LoRetention
pipette tip.
Firmly attach the pipette tips.
Use epT.I.P.S. pipette tips. For
5mL and 10mL
epDualfilterT.I.P.S. pipette
tips, work without a protection
filter in the pipette.
Slowly remove the tip from the
liquid with a time delay (approx.
3 seconds).
Only use accessories and original spare parts recommended by Eppendorf.
10
epT.I.P.S.® — Instructions for use
11 epT.I.P.S. combinations
11 epT.I.P.S. combinations
●●
GELoader
Microloader
1 mL – 10 mL L
●
Eppendorf Reference
0.1 μL – 10 μL S 0.1 μL – 10 μL M 0.1 μL – 20 μL M 0.5 μL – 20 μL L 2 μL – 20 μL 2 μL – 100 μL 2 μL – 200 μL 20 μL – 300 μL 50 μL – 1 000 μL 50 μL – 1 250 μL 50 μL – 1 250 μL L 500 μL – 2 500 μL 100 μL – 5 000 μL 1 mL – 10 mL
Reference
Color dark gray medium gray medium gray light gray yellow yellow yellow orange blue green dark green red violet turquoise light gray
●● ●
●●●●●
●●●● ●
ep Dualfi lter T.I.P.S.
LoRetention
Fixed volume
1 μL, 2 μL, 5 μL, 10 μL
medium gray
10 μL
epT.I.P.S. LoRetention ● ● ● ●
ep Dualfi lter T.I.P.S. ● ● ● ● ● ● ● ● ● ● ●
epT.I.P.S. ● ● ● ● ● ● ● ● ● ● ● ●
yellow
●● ● ●
20 μL
yellow
25 μL, 50 μL, 100 μL
●● ●
●● ●
yellow
200 μL, 250 μL,
500 μL, 1 000 μL
blue
1 500 μL, 2 000 μL,
2 500 μL
red●Variable volume
●●●
●●●● ●
0.1 μL – 2.5 μL
dark gray
0.5 μL – 10 μL
medium gray
○●● ●
2 μL – 20 μL
light gray
●● ●
2 μL – 20 μL
yellow
10 μL – 100 μL
○● ● ●
○○ ● ●
yellow
50 μL – 200 μL
yellow
50 μL – 250 μL
blue●100 μL – 1 000 μL
●● ●
blue
500 μL – 2 500 μL
red
● compatible
○ limited volume
11
epT.I.P.S.® — Instructions for use
●
GELoader
Microloader
1 mL – 10 mL L
●
0.1 μL – 10 μL S 0.1 μL – 10 μL M 0.1 μL – 20 μL M 0.5 μL – 20 μL L 2 μL – 20 μL 2 μL – 100 μL 2 μL – 200 μL 20 μL – 300 μL 50 μL – 1 000 μL 50 μL – 1 250 μL 50 μL – 1 250 μL L 500 μL – 2 500 μL 100 μL – 5 000 μL 1 mL – 10 mL
epT.I.P.S. LoRetention ● ● ● ●
Color dark gray medium gray medium gray light gray yellow yellow yellow orange blue green dark green red violet turquoise light gray
epT.I.P.S. ● ● ● ● ● ● ● ● ● ● ● ● ●
Research
Eppendorf Research
●● ●
●● ●
●● ●
●● ● ●
●●●●●
ep Dualfi lter T.I.P.S.
LoRetention
Fixed volume
10 μL
yellow
20 μL
yellow
25 μL, 50 μL, 100 μL
yellow
200 μL, 250 μL,
ep Dualfi lter T.I.P.S. ● ● ● ● ● ● ● ● ● ● ●
500 μL, 1 000 μL
blue
●●●
Variable volume
0.1 μL – 2.5 μL
dark gray
0.5 μL – 10 μL
●●●● ●
medium gray
●● ● ○
2 μL – 20 μL
yellow
○● ● ●
10 μL – 100 μL
yellow
20 μL – 200 μL
○○ ● ●
○○ ○ ●
yellow
30 μL – 300 μL
orange
●● ●
100 μL – 1 000 μL
blue
500 μL – 5 000 μL
violet●1 mL – 10 mL
turquoise
● compatible
○ limited volume
12
epT.I.P.S.® — Instructions for use
●
GELoader
Microloader
1 mL – 10 mL L
●
●●●●●
0.1 μL – 10 μL S 0.1 μL – 10 μL M 0.1 μL – 20 μL M 0.5 μL – 20 μL L 2 μL – 20 μL 2 μL – 100 μL 2 μL – 200 μL 20 μL – 300 μL 50 μL – 1 000 μL 50 μL – 1 250 μL 50 μL – 1 250 μL L 500 μL – 2 500 μL 100 μL – 5 000 μL 1 mL – 10 mL
ep Dualfi lter T.I.P.S.
LoRetention
Fixed volume
epT.I.P.S. LoRetention ● ● ● ●
ep Dualfi lter T.I.P.S. ● ● ● ● ● ● ● ● ● ● ●
Color dark gray medium gray medium gray light gray yellow yellow yellow orange blue green dark green red violet turquoise light gray
epT.I.P.S. ● ● ● ● ● ● ● ● ● ● ● ● ●
Research plus
Eppendorf Research plus
10 μL
●● ●
●●
●● ●
●● ●
●● ● ●
● ●
●●●● ●
medium gray
20 μL
light gray
10 μL
yellow
20 μL
yellow
25 μL, 50 μL, 100 μL
yellow
200 μL
yellow
200 μL, 250 μL,
500 μL, 1 000 μL
blue
Variable volume
●●●
0.1 μL – 2.5 μL
dark gray
0.5 μL – 10 μL
●● ● ○
○● ● ●
●●●● ●
○○●● ●
medium gray
2 μL – 20 μL
light gray
2 μL – 20 μL
yellow
10 μL – 100 μL
yellow
○○ ● ●
20 μL – 200 μL
yellow
○○ ○ ●
30 μL – 300 μL
orange
●● ●
100 μL – 1 000 μL
blue
500 μL – 5 000 μL
violet●1 mL – 10 mL
turquoise
● compatible
○ limited volume
13
epT.I.P.S.® — Instructions for use
GELoader
Microloader
1 mL – 10 mL L
○● ● ●
○○ ○ ●
●
●●
●● ●
GELoader
Microloader
1 mL – 10 mL L
○● ● ●
○○ ○ ●
●
●●
●● ●
14
Eppendorf Research pro
0.1 μL – 10 μL S 0.1 μL – 10 μL M 0.1 μL – 20 μL M 0.5 μL – 20 μL L 2 μL – 20 μL 2 μL – 100 μL 2 μL – 200 μL 20 μL – 300 μL 50 μL – 1 000 μL 50 μL – 1 250 μL 50 μL – 1 250 μL L 500 μL – 2 500 μL 100 μL – 5 000 μL 1 mL – 10 mL
Research pro
Color dark gray medium gray medium gray light gray yellow yellow yellow orange blue green dark green red violet turquoise light gray
●●●●●
●●●● ●
epT.I.P.S. LoRetention ● ● ● ●
ep Dualfi lter T.I.P.S. ● ● ● ● ● ● ● ● ● ● ●
ep Dualfi lter T.I.P.S.
LoRetention
0.5 μL – 10 μL
medium gray
5 μL – 100 μL
yellow
15/20 μL – 300 μL
orange
50 μL – 1 000 μL
●●●●●
●●●● ●
● compatible
○ limited volume
blue
50 μL – 1 200 μL
green
0.1/0.25 μL – 5 mL
violet
0.1 μL – 10 μL S 0.1 μL – 10 μL M 0.1 μL – 20 μL M 0.5 μL – 20 μL L 2 μL – 20 μL 2 μL – 100 μL 2 μL – 200 μL 20 μL – 300 μL 50 μL – 1 000 μL 50 μL – 1 250 μL 50 μL – 1 250 μL L 500 μL – 2 500 μL 100 μL – 5 000 μL 1 mL – 10 mL
Xplorer
Color dark gray medium gray medium gray light gray yellow yellow yellow orange blue green dark green red violet turquoise light gray
epT.I.P.S. LoRetention ● ● ● ●
Eppendorf Xplorer
ep Dualfi lter T.I.P.S. ● ● ● ● ● ● ● ● ● ● ●
ep Dualfi lter T.I.P.S.
LoRetention
0.5 μL – 10 μL
medium gray
5 μL – 100 μL
yellow
15/20 μL – 300 μL
orange
50 μL – 1 000 μL
blue
50 μL – 1 200 μL
green
0.1/0.25 μL – 5 mL
violet●0.5 mL – 10 mL
turquoise
● compatible
○ limited volume
epT.I.P.S.® — Instructions for use
12 Technical data
12 Technical data
12.1 Ambient conditions
Ambience Only for use in indoors.
Ambient temperature 5°C–40°C
Relative humidity 10%–95%, non-condensing
Atmospheric pressure 79.5kPa–106kPa
Usage up to a height of 2000m above sea level
13 Transport, storage and disposal
13 Transport, storage and disposal
13.1 Storage
Air temperature Relative humidity Atmospheric
In transport packaging -25°C– 55°C 10%– 95%70kPa– 106kPa
Without transport
packaging
-5°C– 45°C 10%– 95%70kPa– 106kPa
pressure
15
Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com
Application Support Europe: Tel: +49 1803 666 789 (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min)
Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA
Tel: +1 516 334 7500 · Toll free phone: +1 800-645-3050 · Fax: +1 516 334 7506 · E-mail: info@eppendorf.com
Your local distributor: www.eppendor f.com/worldwide
North America: Tel: +1 800 645 3050 · E-mail: techserv@eppendorf.com
Asia Pacific: Tel: +60 3 8023 6869 · E-mail: support_asiapacific@eppendorf.com
support@eppendorf.com
Loading...