Eppendorf CellTram vario User Manual
Operating manual
CellTram® Air · Oil · varioseeon p.Fig.Tab.p
O
l
—
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perating manua
5176 900.018
CellTram® Air
CellTram® Oil
CellTram® vario
Operating manual
Copyright© 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.
CellTram, Eppendorf and TransferTip are registered trademarks of Eppendorf AG, Hamburg,
Germany.
Registered trademarks are not marked in all cases with ™ or ® in this manual.
5176 900.018-16/102012
CellTram® Air · Oil · vario — Operating manual
Table of contents
1 Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.1.1 CellTramAir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.1.2 CellTramOil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1.3 CellTramvario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1.4 Universal capillary holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.1.5 Grip head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.1.6 Adapter for Femtotips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2.1 CellTramAir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2.2 CellTramOil/CellTramvario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.3 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.3.1 CellTram Air . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.3.2 CellTram Oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.3.3 CellTram vario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.1 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.2 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.4 Safety instructions on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.2 Mounting the CellTram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.3 Filling oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.3.1 Heating the syringe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.3.2 Connecting the filling tube. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.3.3 Filling the piston chamber and universal capillary holder with oil . . . . . . . . . . 16
4.3.4 Sealing the filling bore. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.3.5 Removing air bubbles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.4 Mounting the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.5 Setting the angle of the CellTram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
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CellTram® Air · Oil · vario — Operating manual
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.1 Holding and transferringcells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.2 Suctioning liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.1 Replacing O-rings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.3 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7.1 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7.1.1 CellTram Air . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7.1.2 CellTram Oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7.1.3 CellTram vario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7.2 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.2.1 CellTram Air . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.2.2 CellTram Oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.2.3 CellTram vario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.2.4 Pressure tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.3 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
7.3.1 CellTram Air . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
7.3.2 CellTram Oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
7.3.3 CellTram vario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
8 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8.1 CellTram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8.3 Capillaries. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
9 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
9.1 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
9.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
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CellTram® Air · Oil · vario — Operating manual
1 Operating instructions
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time. Please also
note the operating instructions for the accessories, if applicable.
This operating manual is part of the product. Please keep it in a place that is easily
accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. You will find the current version on our
webpage www.eppendorf.com/worldwide.
1.2 Danger symbols and danger levels
The safety precautions in these instructions have the following danger symbols and danger
levels:
1.2.1 Danger symbols
Hazard point Cuts
Material damage
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WARNING Can lead to severe injuries or death.
CAUTION Can lead to light to moderate injuries.
ATTENTION May lead to material damage.
1.3 Symbols used
Symbol Meaning
Handling
1.
2.
Actions in the specified order
• List
Text Name of fields in the software
Useful information
5
CellTram® Air · Oil · vario — Operating manual
2 Product description
2 Product description
2.1 Main illustration
2.1.1 CellTramAir
Abb. 1:Side view
1 2 3 4 5 6 7 8
Fig. 1: Side view
1 Rotary knob 2 Scale (from 0 to 9)
3 Tilting joint 4 Piston window
5 Foot 6 Ventilation screw
7 Barb screw 8 Pressure tubing
Abb. 2:Top view
1 2
15
10
0
5
Fig. 2: Top view
1 Position indicator 2 Piston window with scale range
6
CellTram® Air · Oil · vario — Operating manual
2.1.2 CellTramOil
Abb. 3:Side view
1
2 3 4 5 6 7 8
9
10
Fig. 3: Side view
1 Pressure tubing 2 Barb screw
3 Cylinder body (transparent) 4 Filling bore with filling screw
5 Piston (in initial position) 6 Housing
7 Scale 8 Rotary knob
9 Tilting joint 10 Foot
2.1.3 CellTramvario
Abb. 4:Side view
1
2 3 4 5 6 7 8 9
10
11
Fig. 4: Side view
1 Pressure tubing 2 Barb screw
3 Cylinder body (transparent) 4 Filling bore with filling screw
5 Piston (in initial position) 6 Housing
7 Rotary knob, coarse setting 8 Scale
9 Rotary knob, fine setting 10 Tilting joint
11 Foot
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CellTram® Air · Oil · vario — Operating manual
2.1.4 Universal capillary holder
Abb. 5:Universal capillary holder
1 2 3 4 5
Fig. 5: Universal capillary holder
1 Barb 2 Rear knurled screw
3 Tension piece 4 Front knurled screw
5 Grip head0 for capillaries with an outer
diameter of 1.0 to 1.1mm
2.1.5 Grip head
Abb. 6:Grip head 0 with O-rings and sealing washer
Fig. 6: Grip head 0 with O-rings and sealing washer
The transparent grip head allows the user to visually monitor the position of the capillary.
The capillary is fixed in the universal capillary holder via the grip head.
2.1.6 Adapter for Femtotips
Abb. 7:Adapter for Femtotips with spare O-rings
Fig. 7: Adapter for Femtotips with spare O-rings
8
CellTram® Air · Oil · vario — Operating manual
2.2 Features
The CellTramAir, CellTramOil and CellTramvario are manual piston pumps. Using a movable
piston, the CellTram generates differences in pressure which are transferred directly to a capillary
via a pressure tube. The piston is moved in the CellTram using the dial. A clockwise rotation, i.e.
to the right, results in an increase in pressure, the capillary contents are pressed out. A
counterclockwise rotation, i.e. to the left, results in a decrease in pressure, aspiration into the
capillary takes place. To assist fine pressure adjustment, the dial has a scale.
The capillary is inserted into a capillary holder which in turn provides the connection to the
pressure tube. The universal capillary holder enables permanent mounting of various capillaries
in the laboratory.
The CellTramAir, CellTramOil and CellTramvario have a very ergonomic design which enables
easy handling, particularly in conjunction with all control units of Eppendorf micromanipulators.
2.2.1 CellTramAir
The CellTramAir uses air for pressure transmission. This guarantees clean and simple handling
of the piston pump.
A complete revolution of the dial corresponds to a change in volume of approx. 88L.
The position of the piston is displayed in the piston window on a scale from 0 to 15mm
corresponding to the strokes.
2.2.2 CellTramOil/CellTramvario
The CellTramOil and CellTramvario use oil for pressure transmission which, unlike air, can
hardly be compressed. This ensures its operating cycles are very controlled and constant. The
simple filling technique reduces the filling time and simplifies oil filling. To ensure controlled,
constant pressure transmission, the cylinder, pressure tube and part of the capillary are filled with
oil. A complete revolution of the dial (coarse drive) corresponds to a change in volume of approx.
9.6L.
The CellTramvario has an additional fine drive dial. The transmission ratio between fine drive
and coarse drive is 10:1. A revolution of the fine drive corresponds to a change in volume of
approx. 0.96L. This enables ultra-fine cell movements.
The position of the piston can be observed in the transparent cylinder. The filling bore with the
plug screw is at the top of the cylinder. The pressure tube is connected to the CellTramOil/
CellTramvario with the aid of the tube connection screw. The pressure tube has a joint at both
ends and thus connects the CellTramOil/CellTramvario and the universal capillary holder.
9
CellTram® Air · Oil · vario — Operating manual
2.3 Delivery package
2.3.1 CellTram Air
QuantityOrder no.
(international)
1 5176 000.017 CellTram Air
1 5176 114.004
1 5176 190.002
1- Allen key (4mm)
1 5176 900.018 Operating Manual CellTram Air/oil/vario
2.3.2 CellTram Oil
QuantityOrder no.
(international)
1 5176 000.025 CellTram Oil
1 5176 114.004
1 5176 190.002
1-
1-
1- Allen key (4mm)
1 5176 900.018 Operating Manual CellTram Air/oil/vario
2.3.3 CellTram vario
QuantityOrder no.
(international)
1 5176 000.033 CellTram vario
1 5176 114.004
1 5176 190.002
Description
Pressure tube
1m
Universal capillary holder
For holding Femtotips, Femtotips II or grip heads
Including grip head 0, adapter for Femtotips and spare O-rings
Description
Pressure tube
1m
Universal capillary holder
For holding Femtotips, Femtotips II or grip heads
Including grip head 0, adapter for Femtotips and spare O-rings
Quick valve system
For simple filling of the CellTram
Consists of a premounted filling screw, disposable syringe and
filling tube with a Luer Lock connector
Bottle with mineral oil
25mL
Description
Pressure tube
1m
Universal capillary holder
For holding Femtotips, Femtotips II or grip heads
Including grip head 0, adapter for Femtotips and spare O-rings
10
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