Eppendorf CellTram vario User Manual

Operating manual

CellTram® Air · Oil · varioseeon p.Fig.Tab.p

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perating manua

5176 900.018

CellTram® Air
CellTram® Oil

CellTram® vario

Operating manual

Copyright© 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.

CellTram, Eppendorf and TransferTip are registered trademarks of Eppendorf AG, Hamburg,
Germany.

Registered trademarks are not marked in all cases with ™ or ® in this manual.

5176 900.018-16/102012

CellTram® Air · Oil · vario — Operating manual

Table of contents

1 Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3 Symbols used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.1.1 CellTramAir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.1.2 CellTramOil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1.3 CellTramvario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1.4 Universal capillary holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.1.5 Grip head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.1.6 Adapter for Femtotips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.2 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2.1 CellTramAir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2.2 CellTramOil/CellTramvario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.3.1 CellTram Air . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.3.2 CellTram Oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.3.3 CellTram vario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.1 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.2 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.3 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.4 Safety instructions on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.2 Mounting the CellTram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.3 Filling oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

4.3.1 Heating the syringe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

4.3.2 Connecting the filling tube. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

4.3.3 Filling the piston chamber and universal capillary holder with oil . . . . . . . . . . 16

4.3.4 Sealing the filling bore. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

4.3.5 Removing air bubbles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.4 Mounting the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.5 Setting the angle of the CellTram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3

CellTram® Air · Oil · vario — Operating manual

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.1 Holding and transferringcells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.2 Suctioning liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.1 Replacing O-rings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.3 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

7 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

7.1 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

7.1.1 CellTram Air . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

7.1.2 CellTram Oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

7.1.3 CellTram vario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

7.2 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.2.1 CellTram Air . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.2.2 CellTram Oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.2.3 CellTram vario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.2.4 Pressure tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.3 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

7.3.1 CellTram Air . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

7.3.2 CellTram Oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

7.3.3 CellTram vario . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

8 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

8.1 CellTram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

8.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

8.3 Capillaries. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

9 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

9.1 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

9.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

4

CellTram® Air · Oil · vario — Operating manual

1 Operating instructions

1 Operating instructions

1.1 Using this manual

 Read this operating manual completely before using the device for the first time. Please also

note the operating instructions for the accessories, if applicable.

 This operating manual is part of the product. Please keep it in a place that is easily

accessible.

 Enclose this operating manual when transferring the device to third parties.
 If this manual is lost, please request another one. You will find the current version on our

webpage www.eppendorf.com/worldwide.

1.2 Danger symbols and danger levels

The safety precautions in these instructions have the following danger symbols and danger
levels:

1.2.1 Danger symbols

Hazard point Cuts

Material damage

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WARNING Can lead to severe injuries or death.

CAUTION Can lead to light to moderate injuries.

ATTENTION May lead to material damage.

1.3 Symbols used

Symbol Meaning

 Handling

1.

2.

Actions in the specified order

• List

Text Name of fields in the software

Useful information

5

CellTram® Air · Oil · vario — Operating manual

2 Product description

2 Product description

2.1 Main illustration

2.1.1 CellTramAir

Abb. 1:Side view

1 2 3 4 5 6 7 8

Fig. 1: Side view

1 Rotary knob 2 Scale (from 0 to 9)

3 Tilting joint 4 Piston window

5 Foot 6 Ventilation screw

7 Barb screw 8 Pressure tubing

Abb. 2:Top view

1 2

15

10

0

5

Fig. 2: Top view

1 Position indicator 2 Piston window with scale range

6

CellTram® Air · Oil · vario — Operating manual

2.1.2 CellTramOil

Abb. 3:Side view

1

2 3 4 5 6 7 8

9

10

Fig. 3: Side view

1 Pressure tubing 2 Barb screw

3 Cylinder body (transparent) 4 Filling bore with filling screw

5 Piston (in initial position) 6 Housing

7 Scale 8 Rotary knob

9 Tilting joint 10 Foot

2.1.3 CellTramvario

Abb. 4:Side view

1

2 3 4 5 6 7 8 9

10

11

Fig. 4: Side view

1 Pressure tubing 2 Barb screw

3 Cylinder body (transparent) 4 Filling bore with filling screw

5 Piston (in initial position) 6 Housing

7 Rotary knob, coarse setting 8 Scale

9 Rotary knob, fine setting 10 Tilting joint

11 Foot

7

CellTram® Air · Oil · vario — Operating manual

2.1.4 Universal capillary holder

Abb. 5:Universal capillary holder

1 2 3 4 5

Fig. 5: Universal capillary holder

1 Barb 2 Rear knurled screw

3 Tension piece 4 Front knurled screw

5 Grip head0 for capillaries with an outer

diameter of 1.0 to 1.1mm

2.1.5 Grip head

Abb. 6:Grip head 0 with O-rings and sealing washer

Fig. 6: Grip head 0 with O-rings and sealing washer

The transparent grip head allows the user to visually monitor the position of the capillary.
The capillary is fixed in the universal capillary holder via the grip head.

2.1.6 Adapter for Femtotips

Abb. 7:Adapter for Femtotips with spare O-rings

Fig. 7: Adapter for Femtotips with spare O-rings

8

CellTram® Air · Oil · vario — Operating manual

2.2 Features

The CellTramAir, CellTramOil and CellTramvario are manual piston pumps. Using a movable
piston, the CellTram generates differences in pressure which are transferred directly to a capillary
via a pressure tube. The piston is moved in the CellTram using the dial. A clockwise rotation, i.e.
to the right, results in an increase in pressure, the capillary contents are pressed out. A
counterclockwise rotation, i.e. to the left, results in a decrease in pressure, aspiration into the
capillary takes place. To assist fine pressure adjustment, the dial has a scale.

The capillary is inserted into a capillary holder which in turn provides the connection to the
pressure tube. The universal capillary holder enables permanent mounting of various capillaries
in the laboratory.

The CellTramAir, CellTramOil and CellTramvario have a very ergonomic design which enables
easy handling, particularly in conjunction with all control units of Eppendorf micromanipulators.

2.2.1 CellTramAir

The CellTramAir uses air for pressure transmission. This guarantees clean and simple handling
of the piston pump.

A complete revolution of the dial corresponds to a change in volume of approx. 88L.
The position of the piston is displayed in the piston window on a scale from 0 to 15mm

corresponding to the strokes.

2.2.2 CellTramOil/CellTramvario

The CellTramOil and CellTramvario use oil for pressure transmission which, unlike air, can
hardly be compressed. This ensures its operating cycles are very controlled and constant. The
simple filling technique reduces the filling time and simplifies oil filling. To ensure controlled,
constant pressure transmission, the cylinder, pressure tube and part of the capillary are filled with
oil. A complete revolution of the dial (coarse drive) corresponds to a change in volume of approx.

9.6L.
The CellTramvario has an additional fine drive dial. The transmission ratio between fine drive

and coarse drive is 10:1. A revolution of the fine drive corresponds to a change in volume of
approx. 0.96L. This enables ultra-fine cell movements.

The position of the piston can be observed in the transparent cylinder. The filling bore with the
plug screw is at the top of the cylinder. The pressure tube is connected to the CellTramOil/
CellTramvario with the aid of the tube connection screw. The pressure tube has a joint at both
ends and thus connects the CellTramOil/CellTramvario and the universal capillary holder.

9

CellTram® Air · Oil · vario — Operating manual

2.3 Delivery package

2.3.1 CellTram Air

QuantityOrder no.

(international)

1 5176 000.017 CellTram Air

1 5176 114.004

1 5176 190.002

1- Allen key (4mm)

1 5176 900.018 Operating Manual CellTram Air/oil/vario

2.3.2 CellTram Oil

QuantityOrder no.

(international)

1 5176 000.025 CellTram Oil

1 5176 114.004

1 5176 190.002

1-

1-

1- Allen key (4mm)

1 5176 900.018 Operating Manual CellTram Air/oil/vario

2.3.3 CellTram vario

QuantityOrder no.

(international)

1 5176 000.033 CellTram vario

1 5176 114.004

1 5176 190.002

Description

Pressure tube

1m

Universal capillary holder

For holding Femtotips, Femtotips II or grip heads
Including grip head 0, adapter for Femtotips and spare O-rings

Description

Pressure tube

1m

Universal capillary holder

For holding Femtotips, Femtotips II or grip heads
Including grip head 0, adapter for Femtotips and spare O-rings

Quick valve system

For simple filling of the CellTram
Consists of a premounted filling screw, disposable syringe and
filling tube with a Luer Lock connector

Bottle with mineral oil

25mL

Description

Pressure tube

1m

Universal capillary holder

For holding Femtotips, Femtotips II or grip heads
Including grip head 0, adapter for Femtotips and spare O-rings

10

CellTram® Air · Oil · vario — Operating manual

QuantityOrder no.

(international)

1-

1-

1- Allen key (4mm)

1 5176 900.018 Operating Manual CellTram Air/oil/vario

Description

Quick valve system

For simple filling of the CellTram
Consists of a premounted filling screw, disposable syringe and
filling tube with a Luer Lock connector

Bottle with mineral oil

25mL

11

CellTram® Air · Oil · vario — Operating manual

3 Safety

3 Safety

3.1 User profile

The device and accessories may only be operated by trained and skilled personnel.
Before using the device, read the operating manual carefully and familiarize yourself with the

device's mode of operation.

3.2 Intended use

The CellTram has been designed and manufactured for use in the field of Intracytoplasmic sperm
injection (ICSI) in conjunction with Eppendorf VacuTips and Transfertips. The CellTram is thus
considered a medical device within the scope of Directive 93/42/EEC.

The CellTram is intended exclusively for indoor use, and for operation by qualified staff.

3.3 Warnings for intended use

WARNING! Risk of injury due to flying capillaries and glass splinters.

If exposed to high pressures, capillaries can detach themselves from the grip heads and

WARNING!

become projectiles.
Capillaries can crack as a result of incorrect handling.

 Wear protective goggles.
 Never aim capillaries at people.
 Use capillaries with an outer diameter that matches the grip head specifications.
 Always mount/dismount capillaries when they are depressurized.
 Mount the capillary correctly in the grip head.
 Do not touch the capillary with the Petri dish or other objects.

12

WARNING! Damage to health from infectious liquids and pathogenic germs.

 When handling infectious liquids and pathogenic germs, observe the national

WARNING!

regulations, the biological security level of your laboratory, the material safety data
sheets, and the manufacturer's application notes.

 Wear personal protective equipment.
 Comprehensive regulations on handling germs or biological material in risk group II

or higher can be found in the "Laboratory Biosafety Manual" (source: World Health
Organization, Laboratory Biosafety Manual, in the currently valid version).

WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.

 Wear personal protective equipment.

WARNING!

 Observe the national regulations for handling these substances.
 Observe the material safety data sheets and manufacturer's application notes.

CellTram® Air · Oil · vario — Operating manual

CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf

CAUTION!

may impair the safety, functioning and precision of the device. Eppendorf cannot be
held liable or accept any liability for damage resulting from the use of incorrect or
non-recommended accessories and spare parts, or from the improper use of such
equipment.

 Only use accessories and original spare parts recommended by Eppendorf.

3.4 Safety instructions on the device

Depiction Meaning

WARNING

General hazard point. Follow the operating manual.

13

CellTram® Air · Oil · vario — Operating manual

4 Installation

4 Installation

4.1 Selecting the location

Please select the location according to the following criteria:

• Stable

• Level

• Horizontal

• Vibration-damped

4.2 Mounting the CellTram

Valid for:

• CellTram Air

• CellTram Oil

• CellTram vario

Abb. 8:ExampleCellTram Air

1 2 3

Fig. 8: ExampleCellTram Air

1 Pressure tubing 2 Universal capillary holder

For holding Eppendorf capillaries (e.g.,
TransferTips, VacuTips)

3 Grip head0

For capillaries with an outer diameter of

1.0mm to 1.1mm

1. Screw the pressure tubing on to the CellTram.

2. Screw the pressure tubing on to the universal capillary holder.

14

CellTram® Air · Oil · vario — Operating manual

4.3 Filling oil

Valid for:

• CellTram Oil

• CellTram vario

Work with an air cushion in the visible area of the capillary in order to avoid contact

Hint!

between the oil and the medium.

Observe the legal regulations for purity of the oil. The supplied oil is generally used in

Hint!

the ICSI area to coat drops containing embryos/ egg cells or sperm. The supplied oil is
not sterile. Additional instructions can be found in the safety data sheet of the
manufacturer.

Aspirating oil with the syringe may lead to the formation of tiny air bubbles. This should

Hint!

be avoided as these air bubbles may be transferred to the CellTram, which may impair
or slow down the filling process. If the syringe contains air bubbles, these should be
collected by long-term storage. Large air bubbles can be pressed out during filling.

Abb. 9:CellTram OilandCellTram vario

1 2 3 4 5 6

Fig. 9: CellTram OilandCellTram vario

1 Initial position of piston 2 Filling bore with plug screw

3 Final position of piston 4 Barb screw

5 Pressure tubing connection to the rear

6 Grip head 0 for capillary

knurled screw

15

CellTram® Air · Oil · vario — Operating manual

4.3.1 Heating the syringe

1. Screw the filling hose on to the tip.

2. Aspirate at least 3mL of oil into the syringe.

4.3.2 Connecting the filling tube

1. Turn the rotary knob to move the piston to
the initial position.

2. Unscrew the blind screw.

3. Screw the filling tube in the filling bore.

4.3.3 Filling the piston chamber and universal capillary holder with oil

Prerequisites

The piston is in the starting position.

1. Hold the CellTram vertically.

2. Push some oil into the piston chamber.
During the first filling, air bubbles may be

present between the piston and the cylinder.

16

3. Allow these bubbles to rise by moving the
rotary knob or coarse drive back and forth.

CellTram® Air · Oil · vario — Operating manual

4. Completely fill the piston chamber with oil.
Make sure that there are no air bubbles in
the tip of the piston chamber.

5. Completely fill the universal capillary holder
with oil.

6. Visually check that no air bubbles remain in
the universal capillary holder.

4.3.4 Sealing the filling bore

1. Position the CellTram horizontally.

2. Unscrew the filling tube.

3. Fill the filling bore with oil.

17

CellTram® Air · Oil · vario — Operating manual

4. Screw on the blind screw. When doing so,
make sure that no new air bubbles enter the
bore.

5. Soak up any excess oil using a soft
cellulose cloth.

4.3.5 Removing air bubbles

1. If there are still air bubbles in the piston
chamber: slant the CellTram to allow air
bubbles to escape to the filling bore.
Remove the air bubbles.

4.4 Mounting the capillary

WARNING! Risk of injury due to flying capillaries and glass splinters.

If exposed to high pressures, capillaries can detach themselves from the grip heads and

WARNING!

become projectiles.
Capillaries can crack as a result of incorrect handling.

 Wear protective goggles.
 Never aim capillaries at people.
 Use capillaries with an outer diameter that matches the grip head specifications.
 Always mount/dismount capillaries when they are depressurized.
 Mount the capillary correctly in the grip head.
 Do not touch the capillary with the Petri dish or other objects.

18

CAUTION! Risk of cuts when unpacking the capillaries

Capillaries can break as a result of incorrect unpacking.

CAUTION!

 Do not reach into the capillary transport protection.

Grip head 0 is only for capillaries with an outer diameter of 1.0mm and 1.1mm. Other

Hint!

capillaries may only be used with the corresponding grip head (see Ordering
information) (see Accessories on p. 27).

CellTram® Air · Oil · vario — Operating manual

1. Slightly screw the grip head into the
universal capillary holder.

2. Slide the capillary through the inner O-rings up to the stop into the universal capillary holder.

No stop is felt with capillaries with an inner diameter <0.8mm.

Hint!

3. Tighten the grip head.

4. In the case of CellTram Oil and CellTram vario, carefully wipe any excess oil off of the
universal capillary holder and the capillary using a soft cellulose cloth if necessary.

When using Eppendorf Femtotips

1. Screw the Femtotip adapter into the universal capillary holder.

2. Screw the Femtotip into the Femtotip adapter.

4.5 Setting the angle of the CellTram

1. Loosen the fastening screw in the tilting joint with an Allen key (4mm).

2. Set the CellTram via the tilting joint.

3. Tighten the fastening screw in the tilting joint with an Allen key (4mm).

19

CellTram® Air · Oil · vario — Operating manual

5 Operation

5 Operation

5.1 Holding and transferringcells

CellTramAir:

Hint!

An overpressure or vacuum can be built up in the capillary using the volume of the
piston chamber (cylinder) and the pressure tube. Therefore, the pressure behavior
varies depending on the position of the piston. If the piston is located, e.g., in position
10to 15, the differences in pressure are greater than, e.g., in position 0 to 5. By
carefully selecting the position of the piston, the sensitivity of the CellTramAir piston
pump can be adapted to your requirements.

1. Fix the universal capillary holder into the tool holder of the Eppendorfmicromanipulator.

2. Determine the correct length of the capillary by moving the universal capillary holder in the
tool holder.

3. Determine the correct rotation of the capillary by turning the front knurled screw.

4. Move the micromanipulator upwards (see Operating Manual of the micromanipulator).

5. Position the petri dish.

6. Move the capillary into the medium and ensure its correct positioning by observing it under a
microscope.

Establishing pressure equilibrium in the capillary

7. You have three options:

• Wait until equilibrium has been established.

• Turn the piston clockwise (i.e. to the right) to establish a counter pressure which

compensates the active capillary force.

• Only CellTramAir: loosen the ventilation screw. Tighten the screw again after pressure

compensation.

8. Move the capillary near to the desired cell.

9. Generate a vacuum by turning the dial counterclockwise (to the left).

In hold mode:

Hint!

Excessive constant pressure can damage the cell. After the cell has reached the
capillary tip, an optimal constant pressure should be set.

In transfer mode:

Hint!

Ensure that pressure is equilibrated sensitively and evenly during cell aspiration into the
capillary.

10. While the cell moves towards the capillary: it may be necessary to turn the dial clockwise (to
the right) to slightly equilibrate the vacuum generated in order that the cell is not fixed too
firmly to the capillary tip.

20

CellTram® Air · Oil · vario — Operating manual

5.2 Suctioning liquid

Valid for:

• CellTramAir

1. Dip the capillary into the liquid that is to be aspirated.

2. Turn the rotary knob counterclockwise to the left (by approx. 2revolutions) to aspirate the
liquid by creating a low pressure.

3. Wait for a short period and then loosen the ventilation screw.

4. Retighten the ventilation screw after pressure compensation.

21

CellTram® Air · Oil · vario — Operating manual

6 Maintenance

6 Maintenance

6.1 Replacing O-rings

If the O-rings on the universal capillary holder or on the grip head are damaged, they can be
replaced.

1. Remove the O-rings and the sealing washer with the extraction tool.

2. Insert the 2 new O-rings and a new sealing washer in the correct order.

3. Make sure that the 2 sealing washers and 2 matching O-rings are correctly arranged in the
grip head.

6.2 Cleaning

NOTICE! Damage from the use of aggressive chemicals.

 Do not use any aggressive chemicals on the device or its accessories, such as

NOTICE!

Hint!

strong and weak bases, strong acids, acetone, formaldehyde, halogenated
hydrocarbons or phenol.

 If the device becomes contaminated with aggressive chemicals, clean it

immediately using a mild cleaning agent.

Choose the disinfection method which corresponds to the legal regulations and
guidelines in place for your range of application.

If you have any questions on cleaning, disinfection, decontamination, or the cleaning
agents to be used, please contact Eppendorf AG Application Support. The relevant
contact details are provided on the back of this operating manual.

1. Clean the CellTram, pressure tube and universal capillary holder with water or a mild lab
detergent.

2. To remove residual liquid, blow out the pressure tube and universal capillary holder with
compressed air after cleaning.

There must not be any residual liquid remaining in the pressure tube or in the universal

Hint!

capillary holder after cleaning as otherwise steady pressure behavior will be
compromised.

6.3 Disinfection/decontamination

NOTICE! Damage from UV and other high-energy radiation.

 Do not use UV, beta, gamma, or any other high-energy radiation for disinfecting.

NOTICE!

 Avoid storage in areas with strong UV radiation

NOTICE! Damage to the device due to autoclaving.

 Do not autoclave the CellTram.

NOTICE!

22

CellTram® Air · Oil · vario — Operating manual

Required equipment

• Alcohol (ethanol, isopropanol) or disinfectants containing alcohol

Proceed as follows:

1. Choose the disinfection method which corresponds to the legal regulations and guidelines in
place for your range of application.

2. Wipe all surfaces of the CellTram and accessories using the disinfectant.

3. Flush the pressure tube and universal capillary holder with the disinfectant if required.

4. To remove residual liquid, blow out the pressure tube and universal capillary holder with
compressed air after cleaning.

There must not be any residual liquid remaining in the pressure tube or in the universal

Hint!

capillary holder after cleaning as otherwise steady pressure behavior will be
compromised.

23

CellTram® Air · Oil · vario — Operating manual

7 Technical data

7 Technical data

7.1 Ambient conditions

7.1.1 CellTram Air

Ambience For indoor use only

Ambient temperature max. 40°C

Max. relative humidity Max. 80%

Maximum pressure 2900hPa

7.1.2 CellTram Oil

Ambience For indoor use only

Ambient temperature max. 40°C

Max. relative humidity Max. 80%

Maximum pressure Approx. 20000hPa

7.1.3 CellTram vario

Ambience For indoor use only

Ambient temperature max. 40°C

Max. relative humidity Max. 80%

Maximum pressure Approx. 20000hPa

24

CellTram® Air · Oil · vario — Operating manual

7.2 Weight/dimensions

7.2.1 CellTram Air

Footprint (w/o pressure tubing) 175mm×60mm

Weight Approx. 1.4kg

Rotary knob diameter 40mm

7.2.2 CellTram Oil

Footprint (w/o pressure tubing) 275mm×60mm

Weight Approx. 1.7kg

Rotary knob diameter 40mm

7.2.3 CellTram vario

Footprint (w/o pressure tubing) 29.5x6cm

Weight Approx. 1.7kg

Rotary knob diameter (coarse) 42mm

Rotary knob diameter (fine) 34mm

7.2.4 Pressure tubing

Material Polyethylene

Length 1m

Inner diameter 1mm

25

CellTram® Air · Oil · vario — Operating manual

7.3 Application parameters

7.3.1 CellTram Air

Change in volume
(per rotary knob revolution):

Minimum adjustment volume < 0.2L

Adjustable total volume 2.640L

Entire stroke of the piston 15mm (corresponds to 30 revolutions)

Generation of pressure Using piston and cylinder

7.3.2 CellTram Oil

Change in volume
(per rotary knob revolution):

Minimum adjustment volume: <0.02L

Adjustable total volume 960L

Total filling volume
(incl. 1m tubing and universal

capillary holder)

Entire stroke of the piston 50mm (corresponds to 100 revolutions)

Generation of pressure Using piston and cylinder

7.3.3 CellTram vario

Change in volume (per rotary knob
revolution)

Minimum adjustment volume Coarse: <0.02L

Adjustable total volume 960L

Total filling volume
(incl. 1m tubing and universal

capillary holder)

Entire stroke of the piston 50mm (corresponds to 100 revolutions)

88L

9,6L

<2mL

Coarse: 9.6L
Fine: 0.96L

Fine: <0.002L

<2mL

Generation of pressure Using piston and cylinder

26

CellTram® Air · Oil · vario — Operating manual

8 Ordering information

8 Ordering information

8.1 CellTram

Order no.
(international)

5176 000.017 CellTram Air

5176 000.025 CellTram Oil

5176 000.033 CellTram vario

Description

8.2 Accessories

Order no.
(international)

5176 114.004

5176 190.002

5176 210.003

5176 212.006

5176 214.009

5176 207.002

5176 196.000

5176 195.004

5176 120.004

5176 220.009

Description

Pressure tube

1m

Universal capillary holder

For holding Femtotips, Femtotips II or grip heads
Including grip head 0, adapter for Femtotips and spare O-rings

Grip head 0

For microcapillaries with an outer diameter of 1.0mm to 1.1mm, setof2

Grip head 1

For microcapillaries with an outer diameter of 1.2mm to 1.3mm, setof2

Grip head 2

For microcapillaries with an outer diameter of 1.4mm to 1.5mm, setof2

Grip head 3

For microcapillaries with an outer diameter of 0.7mm to 0.9mm, setof2

O-ring set

For grip heads 0-3 with removal tool

Service kit

For grip heads and capillary holder
Consisting of O-ring set, adapter for Femtotips

Filling hose for Quick Valve-system

With a Luer Lock connector (female), 12cm tube coupling for pressure tube
for extending/connecting 2pressure tubes

Tube coupling

For extending or connecting pressure tubes

27

CellTram® Air · Oil · vario — Operating manual

8.3 Capillaries

Various capillaries for sperm injection and the containment of cells are available. VacuTips have
been optimized for holding suspension cells (e.g., oocytes or blastocysts). TransferTips have
been especially developed for transferring sperm in ICSI applications.

Microcapillary Technical data

VacuTip

Microcapillary for holding suspension cells.

TransferTip RP (ICSI)

Microcapillary for injecting human sperm
(ICSI).

TransferTip F (ICSI)

Microcapillary for injecting human sperm
(ICSI).

TransferTip R (ICSI)

Microcapillary for injecting human sperm
(ICSI).

• Sterile

• 15m inner diameter

• 100m outer diameter

• 35° capillary angle

• 1mm flange

• Sterile

• 4m inner diameter

• 7m outer diameter

• 35° capillary angle

• Rigid 0.5mm parallel flange

• Sterile

• 4m inner diameter

• 7m outer diameter

• 35° capillary angle

• Flexible 0.4mm flange

• Sterile

• 4m inner diameter

• 7m outer diameter

• 35° capillary angle

• Rigid 1mm flange

Order no.
(international)

5175 108.000

5175 114.000

5175 106.008

5175 113.004

28

Description

VacuTip

25pieces, sterile

TransferTip-RP (ICSI)

25pieces, sterile

TransferTip-F (ICSI)

25pieces, sterile

TransferTip-R (ICSI)

25pieces, sterile

CellTram® Air · Oil · vario — Operating manual

9 Transport, storage and disposal

9 Transport, storage and disposal

9.1 Storage

Air temperature Relative humidity Atmospheric

In transport packaging -25°C– 55°C 10%– 95%70kPa– 106kPa

Without transport
packaging

-5°C– 45°C 10%– 95%70kPa– 106kPa

pressure

9.2 Decontamination before shipment

If you are shipping the device to the authorized Technical Service for repairs or to your authorized
dealer for disposal please note the following:

WARNING! Risk to health from contaminated device

WARNING!

1. Please observe the information in the decontamination certificate, which is available
on our webpage as a PDF (www.eppendorf.com/decontamination).

2. Decontaminate all the parts you would like to dispatch.

3. Include the fully completed decontamination certificate in the package.

29

Eppendorf AG · 22331 Hamburg · Germany · Tel: [phone] · Fax: [phone] · E-mail: [e-mail]

Application Support Europe: Tel: [phone] (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min)

Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA

Tel: [phone] · Toll free phone: [phone] · Fax: [phone] · E-mail: [e-mail]

Your local distributor: www.eppendor f.com/worldwide

North America: Tel: [phone] · E-mail: [e-mail]

Asia Pacific: Tel: [phone] · E-mail: [e-mail]

[e-mail]