Eppendorf Cell Imagine Plates User Manual
Instructions for use Eppendorf Cell Imaging Platesseeon p.Fig.Tab.p.
English (EN)
Instructions for use
Eppendorf Cell Imaging Plates
English (EN)
1 Product description
The Eppendorf Cell Imaging Plate with glass bottom and the Eppendorf Cell Imaging
Plate with film bottom are suitable for growth and microscopy of cells. The black plates
consist of 24 or 96 wells and are particularly suitable for the use of fluorescent dyes with
cells.
Due to the extremely flat bottom materials glass and film, the Eppendorf Cell Imaging
Plates allow the precise microscopy of cells. The film bottom plate is also suitable for
measurements in the UV wavelength range.
The field of application of Eppendorf Cell Imaging Consumables is training, routine and
research laboratories in the areas of life science, industry, clinics or chemistry. The
Eppendorf Cell Imaging Consumables may only be operated by skilled personnel who
have been trained in the areas mentioned above.
2 Notes for use
WARNING! Risk of contamination
Consumables are only sterile in sealed packaging.
Please check that the packaging is undamaged.
Observe the expiry date printed on the packaging.
Only open the packaging immediately before use.
Only use visually perfect and undamaged items.
WARNING! Risk of contamination from multiple use.
Consumables are intended for single-use only.
After use, dispose of consumables in accordance with the substances with
which they have come into contact.
WARNING! Contamination risk from damaged plates during centrifugation.
The plates withstand heavy loads during centrifugation. However, they can be
destroyed and release the substances they contain if used improperly.
Always observe the maximum permitted centrifugation forces.
Read the operating manual of the centrifuge used.
Centrifuge stacked plates at lower speed only.
Note that organic solvents may reduce the mechanical load capability of the
plates. If in doubt please contact the Eppendorf Application Support
(support@eppendorf.com).
Instructions for use
2
Eppendorf Cell Imaging Plates
English (EN)
CAUTION! Risk of injury from glass fragments!
Glass fragments can cause cuts.
Handle consumables made from glass with care. Avoid glass breakage.
Do not touch glass fragments with your hands. Use suitable tools to pick up
glass fragments.
Reagents, consumables contaminated by reagents and materials used for cleaning and
disinfecting must be disposed of in accordance with laboratory regulations.
The surface is tissue culture treated (TCT). The surface should be coated with an
appropriate biological coating (e.g. collagen) to support cell attachment and growth.
Organic solvents may affect the integrity of the adhesive. When using aggressive
chemicals (e.g., acetone) please contact the Eppendorf Application Support
support@eppendorf.com).
(
Extreme temperatures (e.g., when deepfreezing or autoclaving) may result in changes
of the material and can change the mechanical resistance and dimensions of the
Eppendorf Cell Imaging Plates.
3 Technical data
Glass bottom Film bottom
Thickness 170 µm 25 µm
Material Borosilicate Fluorocarbon
Hydrolytic class 1 -
Refraction index 1.52 1.34
Abbe coefficient 55 70
Recommended
working volume
Total volume 24-well: 1.88 mL
Growth area
Centrifugability Can be centrifugued up to 300 × g*
Operational
temperature
24-well: 0.5 mL to 1 mL
96-well: 0.1 mL to 0.2 mL
96-well: 0.428 mL
2
24-well: 1.4 cm
96-well: 0.3 cm
-20° C to +50° C
2
* The centrifugation stability of each plate type generally depends on the centrifuge and
its accessories, the ambient conditions and the liquid used.
The Eppendorf Cell Imaging Plate are packed individually and sterile.
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