Operation Manual
Fraction Collector
C�660
096969
en
Table of contents
Table of contents
1 About this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
1.1 Reference documents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
1.2 Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Abbreviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
2.1 User qualification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.2 Proper use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.3 Improper use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.4 Warning notices used in this manual . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.5 Product safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
2.5.1 Instrument-related hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.5.2 Other hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
2.5.3 Safety measures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.6 General safety rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1 Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1.1 Standard instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
3.1.2 Standard accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1.3 Optional accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.2 Materials used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3 Technical data overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4 Description of function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.1 Overview over the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.2 Function prinicple . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
5 Putting into operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.1 Installation site. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.2 Electrical connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.3 Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.3.1 Power connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.3.2 Fitting and ferrule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.3.3 Optional waste diverter valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Read this manual carefully before installing and running your system and note the safety precautions
in chapter 2 in particular. Store the manual in the immediate vicinity of the instrument, so that it can be
consulted at any time.
No technical modifications may be made to the instrument without the prior written agreement of
BUCHI. Unauthorized modifications may affect the system safety or result in accidents.
This manual is copyright. Information from it may not be reproduced, distributed, or used for competitive purposes, nor made available to third parties. The manufacture of any component with the aid of
this manual without prior written agreement is also prohibited.
The English manual is the original language version and serves as basis for all translations
into other languages. Other language versions can be downloaded at www.buchi.com.
3 C-660 Operation Manual, Version F
Table of contents
6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.1 Control elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.2 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
6.3 Operating the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6.3.1 Remote Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6.3.2 Manual control / manual mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.3.3 Setting the height of the collector arm . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.3.4 Interface RS 232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.3.5 Peak detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
7.1 Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
8.1 Malfunctions and their remedy. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
8.2 Customer service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9 Shutdown, storage, transport and disposal . . . . . . . . . . . . . . . . . . . . . . . . 31
9.1 Storage and transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
9.2 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
10 Spare parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
11 Declarations and requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
11.1 FCC requirements (for USA and Canada) . . . . . . . . . . . . . . . . . . . . . . . 33
11.2 Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
4 C-660 Operation Manual, Version F
1 About this manual
This manual describes the Fraction Collector C-660 and provides all information required for its safe
operation and to maintain it in good working order.
It is addressed in particular to laboratory personnel and operators.
NOTE
The symbols pertaining to safety (WARNINGS and ATTENTIONS) are explained in chapter 2.
1.1 Reference documents
• Installation Guide SepacoreControl
• Pump Modules C-601 & C-605, Pump Controller C-610 / Pump Manager C-615,
Operation Manual numbers 96961 – 96965
• UV-Monitor C-630, Operation Manual numbers 96950 – 96953 and 96966
• UV-Photometer C-635, Operation Manual numbers 96957 – 96960 and 96967
• Cartridger C-670, Operation Manual numbers 96945 – 96949
1 About this manual
1.2 Trademarks
The following product names and any registered and unregistered trademarks mentioned in this
manual are used for identification purposes only and remain the exclusive property of their respective
owners:
• Sepacore® is a registered trademark of BÜCHI Labortechnik AG
• Syncore® is a registered trademark of BÜCHI Labortechnik AG
• Cartridger® is a registered trademark of BÜCHI Labortechnik AG
1.3 Abbreviations
ETFE: Ethylene/Tetrafluoroethylene Copolymer
FEP: Fluorinated Ethylene Propylene
POM: Polyoxymethylene
PUR: Polyurethane
5 C-660 Operation Manual, Version F
2 Safety
This chapter highlights the safety concept of the fraction collector and contains general rules of
behavior and warnings from hazards concerning the use of the product.
The safety of users and personnel can only be ensured if these safety instructions and the safetyrelated warnings in the individual chapters are strictly observed and followed, therefore, the manual
must always be available to all persons performing the tasks described herein.
2.1 User qualification
The instrument may only be used by laboratory personnel or other persons who on account of training
or professional experience have an overview of the dangers which can develop when operating the
instrument.
Personnel without this training or persons who are currently being trained require careful supervision.
The present Operation Manual serves as a basis for training.
2.2 Proper use
2 Safety
The Fraction Collector C-660 has been conceived of and constructed as a laboratory instrument. Its
correct usage is the optimal and reliable collection of individual aqueous and/or organic fractions in
predetermined or customized racks (e.g. chromatography or Syncore racks).
Correct usage of the Fraction Collector C-660 also includes its maintenance and careful handling of
the instrument on the basis of the specifications given in the Operation Manual.
2.3 Improper use
Applications beyond those described above are improper. Furthermore, applications which do not
comply with the technical data are also considered improper. The operator bears the sole risk for any
damages caused by such improper use.
The following applications are expressly forbidden:
• Use of the instrument in rooms which require ex-protected instruments.
• Collection of samples which can explode or inflame due to shock, friction, heat or spark formation
or that are combustible (e.g. explosive substances, etc.)
• Use in human medicine diagnostics.
• Preparation of food.
6 C-660 Operation Manual, Version F
2.4 Warning notices used in this manual
WARNING
Generally, the triangular warning symbol indicates the possibility of personal injury or even loss of life
if the instructions are not followed.
WARNING
Electrical hazard.
WARNING
Harmful to life forms.
WARNING
Inhalation of harmful substances.
ATTENTION
With the “Read this” symbol, ATTENTION indicates the possibility of equipment damage, malfunctions
or incorrect process results if the instructions are not followed.
2 Safety
NOTE
Useful tips for the optimum operation of the instrument.
2.5 Product safety
The Pump System is designed and built in accordance with current state-of-the-art technology,
however, risks to users, property, and the environment can arise when the instrument is used carelessly or improperly.
The manufacturer has determined residual dangers emanating from the instrument
• if the instrument is operated by insufficiently trained personnel.
• if the instrument is not operated according to its proper use.
Appropriate warnings in this manual serve to make the user alert to these residual dangers.
2.5.1 Instrument-related hazards
Pay attention to the following safety notices:
WARNING
Potential risk in case of damaged glassware.
• Beware of shivering glass parts.
• Beware of defective FEP tubing.
• Beware of leaky connections and insufficiently long connection tubings.
• Beware of defective valves.
• Beware of a false positioning of the collector arm
7 C-660 Operation Manual, Version F
WARNING
Potential risk if solvent vapors accumulate within the instrument housing.
• Beware of damaged or cracked glass parts.
• Beware of dangerous, flammable or explosive samples.
• Operate the instrument in a fume hood or in an adequately ventilated work area.
2.5.2 Other hazards
WARNING
Certain solvents within or in the vicinity of the Pump Systems can form peroxides and/or are highly
inflammable.
• Always be aware of the explosion risk when working with hazardous substances or with
substances of unknown composition.
• Always use the instrument in an adequately ventilated work area.
2 Safety
2.5.3 Safety measures
Always wear personal protective equipment such as protective goggles, protective clothing and gloves
when working with the instrument.
2.6 General safety rules
Responsibility of the operator
The head of laboratory is responsible for training his personnel.
The operator shall inform the manufacturer without delay of any safety-related incidents which might
occur during the operation of the instrument. Legal regulations, such as local, state and federal laws
applying to the instrument must be strictly followed.
Duty of maintenance and care
The operator is responsible for ensuring that the instrument is only operated in proper manner and
that maintenance, service, and repairs are performed with care, on schedule and by authorized
personnel only.
Spare parts to be used
Use only recommended consumables and spare parts for maintenance to ensure continued optimum
system performance and reliability. Any modifications to the spare parts used are only allowed with the
prior written permission of the manufacturer.
Modifications
Modifications to the instrument are only permitted after prior consultation with and written approval
obtained from the manufacturer. Modifications and upgrades should only be carried out by an authorized BUCHI technical engineer. The manufacturer reserves the right to decline any claim resulting from
unauthorized modifications.
8 C-660 Operation Manual, Version F
3 Technical data
This chapter introduces the reader to the fraction collector and its main components. It contains technical data, requirements and performance data.
3.1 Scope of delivery
Check the scope of delivery according to the order number.
NOTE
For detailed information on the listed products, see www.buchi.com or contact your local dealer.
3.1.1 Standard instrument
3 Technical data
Table 3-1: Standard instrument
Product Order number
Fraction Collector C-660 44900
3.1.2 Standard accessories
Table 3-2: Standard accessories
4
1
2
3
Product Order number
a FEP tubing, 1/8”×1/16”, 5 m, green
FEP tubing thin, 1/16”×1/32”, 5 m,
gray
b 25 fittings, 1/8”, green
25 fittings, 1/16”, gray
c 25 ferrules, 1/8” green
25 ferrules, 1/16” gray
d Tubing guide 20780
44354
44357
40956
44816
40961
44269
9 C-660 Operation Manual, Version F
3.1.3 Optional accessories
3 Technical data
Table 3-2: Standard accessories (cont.)
Product Order number
Regional power cord, 1.5 m:
Type CH 10010
Type Schuko (D,F) 10016
Type GB 17835
Type USA 10020
Type AUS 17836
Operation Manual:
German 96968
English 96969
Spanish 96970
French 96971
Italian 96972
Table 3-3: Optional accessories
Product Order number
Waste valve, valve for the Fraction
51114
Collector. Prevents dripping during the
fraction change.
Tubing fitting set for connecting the
44021
waste valve.
Waste level sensor, optimal accessory for
45288
the Fraction Collector. Mounted at the top
of the waste bottle the float type switch
prevents the running over of the waste
reservoir. Connector for GL 45 glass
threads.
10 C-660 Operation Manual, Version F
3 Technical data
Table 3-3: Optional accessories (cont.)
Product Order number
e 4 racks FC 12, set of 4 racks FC 12
44957
– each for 12 tubes at 250 mL with a
9
8
10
6
diameter of 40 mm (without tubes).
f 4 racks FC 30, set of 4 racks FC 30
– each for 30 tubes at 50 mL with
diameter of 26 mm (without tubes).
g 4 racks FC 60, Set of 4 racks FC 60
44958
44959
– each for 60 tubes at 20 mL with
5
7
diameter of 17 mm (without tubes).
h 10×250 mL tubes for rack FC 12,
44960
diameter of 39 mm; height of 300 mm
i 20×50 mL tubes for rack FC 30,
44961
diameter of 25 mm; height of 185 mm
j 100×20 mL tubes for rack FC 60,
44962
diameter of 16 mm; height of 160 mm
Chromatography table
54290
Pivoting table for the integration of
two pump modules, detectors, fraction
11
collector including support rod holder.
Chromatography table with monitor
54292
holder
Pivoting table for the compact integra-
12
tion of the Control Unit C-620, two pump
modules, detectors, fraction collector and
PC including monitor.
Easy access and safe working due to
clearly visible tubing guides. PC and
monitor are not included.
11 C-660 Operation Manual, Version F
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