Bio-Rad Microbial Culturing Module User Manual

Microbial Culturing Kit

Catalog #166-5020EDU

explorer.bio-rad.com

Store components of this kit at room temperature.

For technical support, call your local Bio-Rad office or, in the U.S., call 1-800-424-6723

Duplication of any part of this document permitted for classroom use only.

Please visit explorer.bio-rad.com to access our selection of language

translations for Biotechnology Explorer™ kit curricula.

Biotechnology Explorer

™

Table of Contents

Page

Introduction........................................................................................................................i

Kit Inventory Checklist ......................................................................................................1

Safety Issues....................................................................................................................1

Preparation of Liquid Media..............................................................................................2

Preparation of Ampicillin ............................................................................................2

Preparation of LB Broth..............................................................................................2

Preparation of Ampicillin Broth ..................................................................................3

Starting Minipreps ......................................................................................................3

Preparation of Solid Media ..............................................................................................4

Preparation of Ampicillin ............................................................................................4

Preparation of LB Agar ..............................................................................................4

Preparation of LB-Ampicillin Agar ..............................................................................5

Preparation of LB-Ampicillin IPTG Agar ....................................................................5

Procedure for Pouring Plates............................................................................................5

Streaking Plates with Bacteria ..........................................................................................6

Glossary ..........................................................................................................................7

Introduction

The microbial culturing kit provides the materials and methods for a wide variety of
microbiology activities. The kit can be used to make both liquid and solid media suitable for
the growth of small or large cultures of either environmental microbes or laboratory strains
of

E.coli

The microbial culturing kit is also part of the Bio-Rad Cloning and Sequencing Explorer
Series (catalog #166-5000EDU) and contains the materials used in the Bio-Rad Microbes
and Health kit (catalog #166-5030EDU).

For more microbiology curricula visit explorer.bio-rad.com and look for the pGLO™Bacterial
Transformation kit (catalog #166-0006EDU) or the Microbes and Health kit
(catalog #166-5030EDU).

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