Bio-Rad Macro-Prep 25 S Media User Manual

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Macro-Prep® 25
Ion Exchange Supports

Instruction Manual

Table of Contents

Section 1 Introduction ......................................................1

Section 2 Intended Use ....................................................1

Section 3 Technical Specifications and

Characteristics .................................................1

Section 4 Preparation for Use ..........................................4

Section 5 Column Packing................................................4

Section 6 Operation and Maintenance .............................5

Section 7 Technical Assistance........................................9

Section 8 References .......................................................9

Section 9 Ordering Information ......................................10

Section 1
Introduction

Macro-Prep 25 S strong cation exchange support and Macro-Prep 25 Q strong
anion exchange support are designed to provide high resolution and high capacity
for both analytical and preparative separations at high flow rates. Both supports
possess the same rigid, macroporous structure of 50 µm Macro-Prep High Q and
High S supports, but in a 25 µm bead that offers higher resolution. They are easy
to use, allow quick and reliable packing, and can be used for multiple cycles.

Bio-Rad's chromatography supports are manufactured in an ISO 9001 registered
manufacturing facility. Manufacturing processes are audited and registered by
National Quality Assurance Limited under the provisions of ANSI/ISO/ASQ
9001:2000. Bio-Rad Laboratories guarantees quality of product in unopened
containers for 1 year from date of shipment.

Section 2
Intended Use

Macro-Prep 25 ion exchange supports are appropriate for capture, intermediate,
and polishing purification steps that remove contaminants or impurities from
partially purified targets. Due to their rigidity and unique surface chemistry, these
supports are particularly suited for flow-through operations requiring high
throughput and high recovery of target molecules. The slightly hydrophobic base
bead also provides selectivity for removal of trace contaminants such as residual
host cell proteins, endotoxins, and viruses.

All Macro-Prep ion-exchange supports contain carboxyl groups. Depending on the
support, pH conditions, and samples, the supports may act in mixed mode. With
proper planning, this property can be exploited to give unique selectivity, which
may increase yield and purity.

Section 3
Technical Specifications and Characteristics

Macro-Prep 25 ion exchange supports are rigid methacrylate supports, which
exhibit little shrinkage and swelling, making them suitable for medium pressure
applications with a peristaltic or higher pressure pump (see Figure 1). The
Macro-Prep 25 ion exchange supports are not suitable for gravity flow. The
macroporous nature of the support allows both small and large molecules to
access the exchange sites located throughout the support. This results in high
resolution separations with analytical or preparative sample loads.

1

Pressure (psi)

Pressure (bar)

Linear Velocity (cm/hr)

Fig. 1. Linear flow rate vs. operating pressure. The Macro-Prep 25 S support was evaluated for

linear flow rate vs. operating pressure by measuring the pressure at a series of flow rates using 1 M NaCl
on a 1.6 cm (ID) x 12.5 cm (H) column. The pressure plotted was determined by subtracting the pressure
generated by the column when empty from the pressure generated from the column when packed.

The Macro-Prep 25 ion exchange supports are available as the Macro-Prep 25 S
strong cation exchanger and the Macro-Prep 25 Q strong anion exchanger.
Table 1 lists the properties of these supports.

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