Bio-Rad Immun-Blot PVDF Membrane User Manual
Immun-Blot®PVDF
Membrane for
Protein Blotting
Instruction
Manual
Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547
4006127 Rev A
Table of Contents
Section 1 Introduction..............................1
Section 2 Membrane Wetting..................3
Section 3 Dot Blotting ..............................5
Section 4 Electrophoretic Blotting..........6
Section 5 Optimization of Blotting
Section 6 References ..............................10
Section 7 Product Information .............11
Conditions.................................8
Section 1
Introduction
PVDF (polyvinylidene difluoride) membrane was originally introduced to protein use as
an ideal medium for the harsh chemical environment of N-terminal, or Edman degradation,
sequencing. The hydrophobicity of PVDF
makes it an ideal support for binding proteins in
electrophoretic and dot blotting applications.
Because of the hydrophobic nature of PVDF, it
does require a prewetting step in alcohol. The
high protein binding capacity, target retention,
and resistance to cracking has made PVDF an
appealing membrane for general lab techniques.
The two main applications for PVDF are NTerminal Sequencing and Immunoblotting, both
of which benefit from the qualities of PVDF but
in different aspects of the membrane. While
sequencing work is concerned with retaining as
1
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