Bio-Rad IEF and 2-D Standards User Manual

IEF Standards
Instruction Manual
Catalog Number 161-0310
Broad Range pl 4.45-9.6
Product shipped at room temperature.
For research use only.
Store at -20 °C.
Ordering Information
Catalog Number Product Description
Specialty Standards
161-0310 IEF Standards, pI range 4.45-9.6, 250 µl 161-0320 2-D SDS-PAGE Standards, 500 µl
Prestained Standards
161-0324 Kaleidoscope Prestained Standards, 500 µl 161-0325 Kaleidoscope Polypeptide Standards, 500 µl 161-0305 Prestained SDS-PAGE Standards,
low range, 500 µl
161-0309 Prestained SDS-PAGE Standards,
high range, 500 µl
161-0318 Prestained SDS-PAGE Standards,
broad range, 500 µl
SDS-PAGE Molecular Weight Standards
161-0304 SDS-PAGE Standards, low range, 200 µl 161-0303 SDS-PAGE Standards, high range, 200 µl 161-0317 SDS-PAGE Standards, broad range, 200 µl 161-0326 Polypeptide Standards, 200 µl 161-0314 Silver Stain Standards, low range, 200 µl 161-0315 Silver Stain Standards, high range, 200 µl 161-0306 Biotinylated Standards, low range, 250 µl 161-0311 Biotinylated Standards, high range, 250 µl 161-0319 Biotinylated Standards, broad range, 250 µl
Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547
Phone (510) 741-1000
LIT396 Rev F
Cytochrome c
Lentil lectin
Human hemoglobin C
Human hemoglobin A
Equine myoglobin
Equine myoglobin minor band
Human carbonic anhydrase
Bovine carbonic anhydrase
β-Lactoglobulin B
Phycocyanin
Fig. 1. IEF Standards for accurate pI calibration of native IEF gels. A. 5 µl of the standards were run on a horizontal
polyacrylamide IEF gel and stained with Coomassie blue R-250 dye and crocein scarlet. B. 5 µl of the standards were run on a horizontal polyacrylamide IEF gel and left unstained.
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