Bio-Rad HRP and AP Conjugates User Manual

Blotting-Grade
Avidin-Horseradish
Peroxidase Conjugate

Catalog #

170-6528

Specifications

Contents 2 ml

Buffer 10 mM sodium phosphate,

Preparation Avidin is conjugated to horseradish
of conjugate peroxidase using a modification

Recommended 1:1,000 when used in the
working dilution Biotin-Blot

Shelf life 1 year at 4ºC from date thawed

150 mM sodium chloride,
pH 7.2. Contains 1.0%
bovine serum albumin and

0.01% thimerosal.

of the periodate oxidation procedure
described by Wilson and Nakane
(1978).

™

system; 1:3,000 when used
with biotinylated SDS-PAGE
standards. Different dilutions
may be optimal for other
applications.

3 years at –20ºC from date of
manufacture

protein detection

2