Blotting-Grade
Avidin-Horseradish
Peroxidase Conjugate
Catalog #
170-6528
Specifications
Contents 2 ml
Buffer 10 mM sodium phosphate,
Preparation Avidin is conjugated to horseradish
of conjugate peroxidase using a modification
Recommended 1:1,000 when used in the
working dilution Biotin-Blot
Shelf life 1 year at 4ºC from date thawed
150 mM sodium chloride,
pH 7.2. Contains 1.0%
bovine serum albumin and
0.01% thimerosal.
of the periodate oxidation procedure
described by Wilson and Nakane
(1978).
™
system; 1:3,000 when used
with biotinylated SDS-PAGE
standards. Different dilutions
may be optimal for other
applications.
3 years at –20ºC from date of
manufacture
protein detection
2
Storage conditions Store at –20ºC until ready to use. After
Shipping conditions Product ships on dry ice at –20ºC.
Additional The instruction manuals provided with
instructions the biotinylated SDS-PAGE standards
thawing, store at 4ºC to avoid repeated
freeze-thaw cycles.
kit (catalog #161-0307) and Biotin-Blot
protein detection kit (catalog
#170-6512) contain complete details
for assay procedures. To biotinylate
proteins with the Biotin-Blot kit, use
Bio-Rad’s NHS-biotin (catalog
#170-6529).
Reference
Wilson MB and Nakane PK, Immunofluorescence and Related
Staining Techniques (Knapp W et al., eds.) Elsevier/North
Holland Biomedical Press, Amsterdam, 215–224 (1978)
3
Product Information
Catalog # Description
170-6528 Avidin-Horseradish Peroxidase
Bio-Rad Laboratories, Inc.
2000 Alfred Nobel Dr., Hercules, CA 94547 USA
510-741-1000 LIT423 Rev E
Conjugate, 2 ml
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