Page 1
Econo-Column
®
Product Guide
For Technical Service
Call Your Local Bio-Rad Office, or
in the US Call 1-800-4BIORAD
(1-800-424-6723)
Page 2
Page 3
Econo-Column Chemical Resistance Guide
This chart is intended as a guide only. Many factors (concentration, temperature,
intermittent or continuous exposure, etc.) will affect the stability of the plastic
parts. Because of the wide diversity of possible reagents and service conditions,
there is no effective substitute for a practical tri
al.
Chemical Resistance Classification
E Excellent: 30 days of constant exposure causes no damage.
G Good: little or no damage after 30 days of constant exposure to the reagent.
F Fair: some effect after 7 days of constant exposure to the reagent.
N Not recommended for continuous use. Immediate damage may occur.
– No data available.
The first letter of each pair applies to conditions at 20°C; the second to those at 50°C.
Econo-Column Flow Adaptor
Reagent 20°C 50°C 20°C 50°C
Acetaldehyde G N G N
Acetic acid, 5% E E E E
Acetic acid, 50 % E E E E
Acetone N N N N
Acetonitrile F N F N
Aluminum salts E E E E
Ammonia E E E E
Amyl acetate G F E E
Aniline G F G F
Benzaldehyde E G E G
Benzene G F G F
Benzyl alcohol N N N N
n-Butyl acetate G F G F
n-Butyl alcohol E E E E
sec-Butyl alcohol E G E G
tert-Butyl alcohol E G E G
Carbon tetrachloride G F G F
Chlorine, 10% (moist) F N F N
Chloroacetic acid E – E –
Chlorobenzene N N N N
Chloroform G F G F
Chomic acid, 10% E E E E
Chromic acid, 50% G F G F
1
Page 4
Econo-Column Flow Adaptor
Reagent 20°C 50°C 20°C 50°C
Citric acid E E E E
Cresol G F G F
Cyclohexane F N F N
Cyclohexanone F N F N
Dimethyl formamide N N N N
Dimethylsulfoxide - - - Ethyl acetate N N N N
Ethyl alcohol E G E G
Ethyl benzene - - - Ethyl chloride, liquid F N F N
Formaldehyde, 40% E G E G
Formic acid, 98–100% E G E G
Glycerine E E E E
Hydrochloric acid, 35% E G E G
Hydrofluoric acid, 48% E E E E
Hydrogen peroxide, 90% E - E Isobutyl alcohol E E E E
Isopropyl alcohol E E E E
Lactic acid, 85% G F G F
Methylene chloride N N N N
Nitric acid, 10% E E E E
Nitric acid, 50% F N F N
Nitrobenzene N N N N
Perchloric acid G N G N
Phenol, liquid N N N N
Phosphoric acid, 5% E E E E
Phosphoric acid, 85% E G E G
Propionic acid E G E G
Sodium acetate, saturated E E E E
Sodium hydroxide, 1% E E E E
Sodium hydroxide, 50% to saturated E G E G
Sodium hypochlorite G F G F
Sulfuric acid, 6% E E E E
Sulfuric acid, 60% E G E G
Sulfuric acid, 98% F N F N
Toluene G F G F
Trichloroacetic acid F N F N
Trichloroethane N N N N
Trichloroethylene N N N N
Tris buffer, solution E G E G
Urea E E E E
Xylene F N F N
Reprinted by permission of Nalge Company
2
Pressure Stability
Econo-Column chromatography columns are designed for low-pressure applica-
tions, i.e., less than 1.0 atm. Use caution when operating the column with a
pump. A disruption in flow may cause a rapid rise in backpressure. If this occurs,
immediately shut off the pump and check the gel bed for compression.
Temperature Stability
Econo-Column chromatography columns and flow adaptors can be operated at
temperatures up to 50°C. For applications requiring temperature control, jacket-
ed columns are available in various sizes and formats. Refer to the Bio-Rad Life
Sciences Research Products catalog for more information.
Sterilization
Econo-Column chromatography columns and accessories can be sterilized with
2 N NaOH or 100% ethanol. The columns can also be autoclaved, wet or dry, at
a maximum of 15 min at 121°C.
Column Cleaning
Econo-Column chromatography columns can be cleaned with 70% ethanol. The
bed support pore size is 20 µm and may require intermittent cleaning. To clean
the bed support, backwash with 1 N NaOH.
Accessories
A full range of Econo-Column accessories are also available. For more detailed
ordering information, refer to the Bio-Rad Life Science Research Products cata-
log.
Flow Adaptors
Flow adaptors improve resolution and reduce mechanical disruption by deliver-
ing sample and buffer directly to the top of the gel bed.
Catalog# Description
738-0014 Flow Adaptor, 1 cm ID, 7 cm long
738-0015 Flow Adaptor, 1 cm ID, 14 cm long
738-0016 Flow Adaptor, 1.5 cm ID, 14 cm long
738-0017 Flow Adaptor, 2.5 cm ID, 14 cm long
738-0018 Flow Adaptor, 5.0 cm ID, 14 cm long
738-0019 Econo-Pac®Flow Adaptor, 1.5 cm ID, 7 cm long
3
Page 5
Pressure Stability
Econo-Column chromatography columns are designed for low-pressure applications, i.e., less than 1.0 atm. Use caution when operating the column with a
pump. A disruption in flow may cause a rapid rise in backpressure. If this occurs,
immediately shut off the pump and check the gel bed for compression.
Temperature Stability
Econo-Column chromatography columns and flow adaptors can be operated at
temperatures up to 50°C. For applications requiring temperature control, jacketed columns are available in various sizes and formats. Refer to the Bio-Rad Life
Sciences Research Products catalog for more information.
Sterilization
Econo-Column chromatography columns and accessories can be sterilized with
2 N NaOH or 100% ethanol. The columns can also be autoclaved, wet or dry, at
a maximum of 15 min at 121°C.
Column Cleaning
Econo-Column chromatography columns can be cleaned with 70% ethanol. The
bed support pore size is 20 µm and may require intermittent cleaning. To clean
the bed support, backwash with 1 N NaOH.
Accessories
A full range of Econo-Column accessories are also available. For more detailed
ordering information, refer to the Bio-Rad Life Science Research Products catalog.
Flow Adaptors
Flow adaptors improve resolution and reduce mechanical disruption by delivering sample and buffer directly to the top of the gel bed.
Catalog# Description
738-0014 Flow Adaptor, 1 cm ID, 7 cm long
738-0015 Flow Adaptor, 1 cm ID, 14 cm long
738-0016 Flow Adaptor, 1.5 cm ID, 14 cm long
738-0017 Flow Adaptor, 2.5 cm ID, 14 cm long
738-0018 Flow Adaptor, 5.0 cm ID, 14 cm long
738-0019 Econo-Pac®Flow Adaptor, 1.5 cm ID, 7 cm long
3
Page 6
Reservoirs
Reservoirs are ideal for column packing, loading dilute samples, or delivering large quantities of buffer.
Catalog # Description
731-0003 Econo-Column Funnel, 5
737-9112 Econo-Column Glass Reservoir, 500 ml
737-9113 Econo-Column Glass Reservoir, 1,000 ml
Tubing
Bio-Rad offers an extensive line of low-pressure tubing to meet fluid
handling needs.
Catalog # Description
731-8210 Silicone Tubing, 0.8 mm ID/0.8 mm wall, 10 m
731-8211 Silicone Tubing, 1.6 mm ID/0.8 mm wall, 10 m
731-8212 Silicone Tubing, 3.2 mm ID/0.8 mm wall, 10 m
731-8213 Tygon Tubing, 0.51 mm ID/0.8 mm wall, 10 m
731-8214 Tygon Tubing, 0.8 mm ID/0.8 mm wall, 10 m
731-8215 Tygon Tubing, 1.6 mm ID/0.8 mm wall, 10 m
731-8207 PharMed Tubing, 0.8 mm ID/1.0 mm wall, 10 m
731-8208 PharMed Tubing, 1.6 mm ID/1.0 mm wall, 10 m
731-8209 PharMed Tubing, 3.2 mm ID/1.0 mm wall, 10 m
Econo-Column Chemical Resistance Guide
This chart is intended as a guide only. Many factors (concentration, temperature,
intermittent or continuous exposure, etc.) will affect the stability of the plastic
parts. Because of the wide diversity of possible reagents and service conditions,
there is no effective substitute for a practical tri
al.
Chemical Resistance Classification
E Excellent: 30 days of constant exposure causes no damage.
G Good: little or no damage after 30 days of constant exposure to the reagent.
F Fair: some effect after 7 days of constant exposure to the reagent.
N Not recommended for continuous use. Immediate damage may occur.
– No data available.
The first letter of each pair applies to conditions at 20°C; the second to those at 50°C.
Econo-Column Flow Adaptor
Reagent 20°C 50°C 20°C 50°C
Acetaldehyde G N G N
Acetic acid, 5% E E E E
Acetic acid, 50 % E E E E
Acetone N N N N
Acetonitrile F N F N
Aluminum salts E E E E
Ammonia E E E E
Amyl acetate G F E E
Aniline G F G F
Benzaldehyde E G E G
Benzene G F G F
Benzyl alcohol N N N N
n-Butyl acetate G F G F
n-Butyl alcohol E E E E
sec-Butyl alcohol E G E G
tert-Butyl alcohol E G E G
Carbon tetrachloride G F G F
Chlorine, 10% (moist) F N F N
Chloroacetic acid E – E –
Chlorobenzene N N N N
Chloroform G F G F
Chomic acid, 10% E E E E
Chromic acid, 50% G F G F
1
750-0603 FEP Tubing, 1.6 mm ID/1.6 mm wall, 4.6 m
Fittings and Valves
Catalog # Description
731-8220 Econo System Fittings Kit, 250 pieces
Tygon and PharMed are trademarks of Norton Company.
4
Page 7
Page 8
Bio-Rad
Laboratories, Inc.
Econo-Column
®
Product Guide
For Technical Service
Call Your Local Bio-Rad Office, or
in the US Call 1-800-4BIORAD
(1-800-424-6723)
Life Science
Group
Web site www.bio-rad.com USA (800) 4BIORAD Australia 02 9914 2800
Austria (01) 877 89 01 Belgium 09-385 55 11 Brazil 55 21 507 6191
Canada (905) 712-2771 China (86-21) 63052255
Czech Republic (420) 2-4141 0532 Denmark 45 44 52-1000
Finland 358 (0)9 804 2200 France 01 47 95 69 65 Germany 089 318 84-177
Hong Kong 852-2789-3300 India (91-124) 6398112/113/114, 6450092/93
Israel 03 951 4127 Italy 39 02 216091 Japan 03-5811-6270
Korea 82-2-3473-4460 Latin America 305-894-5950
Mexico 52 5 534 2552 to 54 The Netherlands 0318-540666
New Zealand 64-9-4152280 Norway 47-23-38-41-30 Poland (48) 22-8126 672
Portugal 351-21-472-7700 Russia 7 095 721 1404 Singapore 65-2729877
South Africa 00 27 11 4428508 Spain 34 91 590 5200
Sweden 46 (0)8-55 51 27 00 Switzerland 061 717-9555 United Kingdom 0800-181134
00-000 0000 Sig 1001Bulletin 0000 US/EG Rev A
LIT80 Rev E
Loading...