BIO RAD EP-1 Econo Pump Instruction Manual

Model EP-1

Econo Pump

Instruction

Manual

Catalog Numbers

731-8140
731-8142

For Technical Service

Call Your Local Bio-Rad Office or

i

n the U.S. Call 1-800-4BIORAD

(1-800-424-6723)

Table of Contents

Section 1



Safety ..............................................................................1

Section 2



Introduction ...................................................................2

Section 3



Unpacking and Setting Up............................................ 3

3.l



Unpacking Instructions.............................................................3

3.2



Voltage Conversion...................................................................4

Section 4



Physical Description and Control Features ................. 5

4.l



Front Panel Functions...............................................................5

4.2



Rear Panel Functions ................................................................7

4.3



Pump Base Features..................................................................8

Section 5



Tubing Selection and Installation ................................. 8

5.l



Tubing Selection .......................................................................8

5.2



Installation of Precut Bio-Rad Tubing ...................................... 9

5.3



Installation of Uncut Tubing ................................................... 11

Section 6



Operation......................................................................11

6.l



Start-Up Procedure .................................................................I1

6.2



Verification of Pump Software Version ..................................12

6.3



Flow Rate Calibration.............................................................13

6.4



Simple Time-Based Collection with the

Bio-Rad Model 2110 Fraction Collector ................................ 14

Section 7



Cleaning and Maintenance ......................................... 18

Section 8Troubleshooting ...........................................................18

Appendix ATechnical Specifications ...................................„„„„„,20

Appendix BOperation of the Econo Pump When

Connected to Other Instruments ...............................21

Appendix C



Ordering Information ...................................„„„,...„„22

Section 1
Safety

Disconnect supply before servicing. No user serviceable parts inside,
refer servicing to Bio-Rad service personnel.

1

Section 2
Introduction

Section 3

Unpacking and Setting Up

Fig. 2.1.

Model EP-1 Econo Pump.

The Model EP- l Econo Pump is a two-channel, bi-directional, variable

speed peristaltic pump for low-pressure chromatography and general labo
ratory use. It offers a full range of features to facilitate ease of use as a stand
alone pump or as an integral part of the Econo System. As a stand-alone
pump, the Model EP-1 Econo Pump delivers flow rates from 0.l to 40 mumin
with the ability to self-calibrate the flow rate for 0.8, l.6, and 3.2 mm ID
tubing, displaying pump output in ml/min. The pump can be programmed to
control fraction collector parameters such as fraction size, void volume, and
total run volume. A membrane key panel with graphic icon displays allows
easy user interface. When coupled with the Model ES-1 Econo System
Controller, the Model EP-1 Econo Pump will control the gradient run-time,
gradient shape, and peak collection capabilities of the Econo System. When
coupled with the Model ES-1 Econo System Controller and Model EV-

1

Econo Buffer Selector, the Model EP-1 Econo Pump will control up to 5

buffers, automated sample injection, and method cycling.

3.1 Unpacking Instructions

Carefully remove the unit from the shipping box, lifting from the handle
on the back of the pump or from the bottom of the instrument. Remove the
plastic bag and inspect the instrument for any external damage. Parts includ
ed with the Model EP- I Econo Pump are illustrated in Figure 3.l. Check
off all parts against the supplied packing list.

Your Model EP- l Econo Pump was carefully tested at the factory and was
shipped in good working order. If any part is missing or damaged, contact Bio­Rad Laboratories immediately. Refer to Figure 3.1 for the proper identifica­tion designation of any missing or damaged part(s).

Also includes: Instruction Manual

Fig. 3.1. Parts supplied with the Model EP-1 Econo Pump.

3

2

3.2 Voltage Conversion

Warning: The Model EP- I Econo Pump is shipped in its 120 V or 220 V
version. To operate at other voltages, refer to the procedure below. Failure
to follow this procedure may result in damage to the unit and invalida­tion of the warranty.

Prior to connecting the power cord to the power entry module and wall
outlet, verify that the voltage indicated on the power entry module matches
your line voltage. If it does not, use the following procedure to make the
conversion. Refer to Figure 3.2.

1.Disconnect the power cord from the unit.

2.Remove the fuse drawer with a small-blade screwdriver or similar tool.

3.Pull the fuse holder out of the fuse drawer and, if necessary, replace the

fuses with ones having the correct current rating. Use 0.50 A fuses for 100

V and 120 V operation; 0.25 A fuses for 220 V and 240 V operation.

Rotate the voltage selector through the window in the fuse drawer until

the proper voltage shows.

4.Reinsert the fuse drawer into the power entry module, with the locking
tab to the left. The voltage indicator will read right-side-up if the draw­er is oriented correctly. Press gently until it snaps into place.

5.Insert the power cord plug into the power entry module. Plug the powe
cord into a properly grounded

outlet.

Fig. 3.2. Voltage conversion.

Section 4

Physical Description and Control Features

4.1 Front Panel Functions

Fig. 4.1. Front panel controls.

Display



Function

Arrow Keys

The four-character display will show
various parameters during program­ming and operation of the Econo
Pump, Econo System, and Automated
Econo System. The indicator lights
l

ocated immediately to the right of the
display indicate the units displayed:
ml, minutes, ml/minute, or percent.

These keys are used for setting system
parameters when program ming the
system, and for selecting which para­meter to display when running the
system.

4

5

LED Display