BIO RAD EP-1 Econo Pump Instruction Manual

Model EP-1

Econo Pump

Instruction

Manual

Catalog Numbers

731-8140
731-8142

For Technical Service

Call Your Local Bio-Rad Office or

i

n the U.S. Call 1-800-4BIORAD

(1-800-424-6723)

Table of Contents

Section 1



Safety ..............................................................................1

Section 2



Introduction ...................................................................2

Section 3



Unpacking and Setting Up............................................ 3

3.l



Unpacking Instructions.............................................................3

3.2



Voltage Conversion...................................................................4

Section 4



Physical Description and Control Features ................. 5

4.l



Front Panel Functions...............................................................5

4.2



Rear Panel Functions ................................................................7

4.3



Pump Base Features..................................................................8

Section 5



Tubing Selection and Installation ................................. 8

5.l



Tubing Selection .......................................................................8

5.2



Installation of Precut Bio-Rad Tubing ...................................... 9

5.3



Installation of Uncut Tubing ................................................... 11

Section 6



Operation......................................................................11

6.l



Start-Up Procedure .................................................................I1

6.2



Verification of Pump Software Version ..................................12

6.3



Flow Rate Calibration.............................................................13

6.4



Simple Time-Based Collection with the

Bio-Rad Model 2110 Fraction Collector ................................ 14

Section 7



Cleaning and Maintenance ......................................... 18

Section 8Troubleshooting ...........................................................18

Appendix ATechnical Specifications ...................................„„„„„,20

Appendix BOperation of the Econo Pump When

Connected to Other Instruments ...............................21

Appendix C



Ordering Information ...................................„„„,...„„22

Section 1
Safety

Disconnect supply before servicing. No user serviceable parts inside,
refer servicing to Bio-Rad service personnel.

1

Section 2
Introduction

Section 3

Unpacking and Setting Up

Fig. 2.1.

Model EP-1 Econo Pump.

The Model EP- l Econo Pump is a two-channel, bi-directional, variable

speed peristaltic pump for low-pressure chromatography and general labo
ratory use. It offers a full range of features to facilitate ease of use as a stand
alone pump or as an integral part of the Econo System. As a stand-alone
pump, the Model EP-1 Econo Pump delivers flow rates from 0.l to 40 mumin
with the ability to self-calibrate the flow rate for 0.8, l.6, and 3.2 mm ID
tubing, displaying pump output in ml/min. The pump can be programmed to
control fraction collector parameters such as fraction size, void volume, and
total run volume. A membrane key panel with graphic icon displays allows
easy user interface. When coupled with the Model ES-1 Econo System
Controller, the Model EP-1 Econo Pump will control the gradient run-time,
gradient shape, and peak collection capabilities of the Econo System. When
coupled with the Model ES-1 Econo System Controller and Model EV-

1

Econo Buffer Selector, the Model EP-1 Econo Pump will control up to 5

buffers, automated sample injection, and method cycling.

3.1 Unpacking Instructions

Carefully remove the unit from the shipping box, lifting from the handle
on the back of the pump or from the bottom of the instrument. Remove the
plastic bag and inspect the instrument for any external damage. Parts includ
ed with the Model EP- I Econo Pump are illustrated in Figure 3.l. Check
off all parts against the supplied packing list.

Your Model EP- l Econo Pump was carefully tested at the factory and was
shipped in good working order. If any part is missing or damaged, contact Bio­Rad Laboratories immediately. Refer to Figure 3.1 for the proper identifica­tion designation of any missing or damaged part(s).

Also includes: Instruction Manual

Fig. 3.1. Parts supplied with the Model EP-1 Econo Pump.

3

2

3.2 Voltage Conversion

Warning: The Model EP- I Econo Pump is shipped in its 120 V or 220 V
version. To operate at other voltages, refer to the procedure below. Failure
to follow this procedure may result in damage to the unit and invalida­tion of the warranty.

Prior to connecting the power cord to the power entry module and wall
outlet, verify that the voltage indicated on the power entry module matches
your line voltage. If it does not, use the following procedure to make the
conversion. Refer to Figure 3.2.

1.Disconnect the power cord from the unit.

2.Remove the fuse drawer with a small-blade screwdriver or similar tool.

3.Pull the fuse holder out of the fuse drawer and, if necessary, replace the

fuses with ones having the correct current rating. Use 0.50 A fuses for 100

V and 120 V operation; 0.25 A fuses for 220 V and 240 V operation.

Rotate the voltage selector through the window in the fuse drawer until

the proper voltage shows.

4.Reinsert the fuse drawer into the power entry module, with the locking
tab to the left. The voltage indicator will read right-side-up if the draw­er is oriented correctly. Press gently until it snaps into place.

5.Insert the power cord plug into the power entry module. Plug the powe
cord into a properly grounded

outlet.

Fig. 3.2. Voltage conversion.

Section 4

Physical Description and Control Features

4.1 Front Panel Functions

Fig. 4.1. Front panel controls.

Display



Function

Arrow Keys

The four-character display will show
various parameters during program­ming and operation of the Econo
Pump, Econo System, and Automated
Econo System. The indicator lights
l

ocated immediately to the right of the
display indicate the units displayed:
ml, minutes, ml/minute, or percent.

These keys are used for setting system
parameters when program ming the
system, and for selecting which para­meter to display when running the
system.

4

5

LED Display

Run/Stop Key

Purge Key

Direction Key

Tubing Calibration Key

Fraction Collector Key

These lights indicate the units for the
value displayed on the LED display
located to the left. A flashing light
indicates that a value can be set using
the Arrow keys.

This key is used to start or stop the
pump. It also has secondary func­tions such as holding or aborting a
method.

This key is used to prime and purge
tubing lines without disturbing the pri­mary speed setting of the pump. When
Purge is pressed, the pump will run at

maximum speed (25 rpm). The purge
key will not operate during a gradient
or fraction collection method.

This key changes the direction of
pump head rotation. The Econo Pump

must be stopped before the direction
can be changed.

This key is used to select pre-pro­grammed calibration settings for three
tubing sizes, or allows user calibration
for increased flow rate accuracy or
non-standard tubing sizes. The pump
must be stopped for this key to oper­ate.

This key is used to set fraction size,
void volume (to move a diverter valve
to "collect" or "waste" positions), and
total run volume. The total run vol­ume (V) may be used to program the
pump to automatically shut off after a
given volume has been delivered.

4.2 Rear Panel Functions

Fig. 4.2. Rear panel sockets.

Program Run Key

Note: When entering values for tubing calibration or fraction collection
procedures, only the flashing keys are active.

Display

Power Entry Module

Fraction Collector
Output

Diverter Valve Output

This key is used to control a pro­grammed gradient or fraction collec­tion method.

When the indicator light

is

flashing, pressing this key starts the

method.

When the indicator light is

lit,

pressing this key will interrupt the
program, which can be continued by
pressing again.

When the LED dis­plays "FAIL", (indicating a brief
power outage has occurred), pressing
this key continues the program.

Function
Grounded 3-pin receptacle for the

power cord. Also contained within is a
four-position (100 V/120 V/220 V/
240 V) line voltage selector. See
Section 3.2.

This 8-pin connector sends an
advance signal to the Model 2110
Fraction Collector via Econo System
Cable # l.

This 8-pin outlet sends a signal to the
Model SV-3 diverter valve, to divert
flow between collection devices.

Indicator Lights

6 7

5.2 Installation of Precut Bio-Rad Tubing

4.3 Pump Base Features

The base of the Model EP-1 Econo Pump contains a 40-pin connector for
connection to the Model ES-1 Econo System Controller via a ribbon cable
located on the Model ES-1 Econo System Controller. When connected, the
two provide added features such as gradient proportioning and mixing, peak
detection, and control of a waste/collect diverter valve. See Section 6 for
details.

Section 5
Tubing Selection and installation

5.1 Tubing Selection

The Model EP-1 Econo Pump may be used with most flexible tubing
having an inner diameter less than or equal to 3.2 mm (l/8"), and a wall
thickness of 1.0 mm or less, including silicone, Tygon, and PharMed. Silicone
tubing, the most inert of the three, is suitable for aqueous and polar solu­tions. Tygon is suitable for most aqueous solutions. It will generally have
the shortest lifetime of the three. PharMed is the longest lasting of the three

,
and will provide the most consistent flow rate over time. Table 5.l shows
approximate flow rate ranges with different tubing sizes. Flow rates above 20
ml/min may be obtained by plumbing two channels of 3.2 mm tubing through
the pump and joining them at the output.

Table 5.1. Approximate Flow Rate Ranges

Fig. 5.1. Tubing installation.

l.



Referring to Figure 5.l, pull the platen cam lever away from the pump
head to unlock the platen and slide the platen away from the pump head
frame assembly, exposing the rollers.

2.



Slip a lock-ring onto one end of the tubing. See below for the size and

color of each lock-ring. Insert a barbed female luer-fitting into the same
end until the tubing reaches the flange of the fitting. Clamp the luer-fit
ting into place by sliding the lock-ring along the tubing over the barbed
fitting (see Figure 5.2). Repeat on the other end of tubing.

Note: Use of lock-rings is required only when operating at pressures
above 10 psi.

Lock-Ring Color



Tubing ID

Red



0.8 mm (1/32")

Orange



1.6 mm (1/16")

Yellow



3.2 mm (1/8")

This 8-pin connector is for communi­cation with the Model EM- l Econo
UV Monitor.

Auxiliary Output

8

9

Fig. 5.2. Attachment of tubing fittings.

3.Insert one end of the tubing into the tubing retaining bracket of the pump
head. Lightly pull the tubing around the rollers to remove slack. Attach

the other end of tubing into the tubing retaining bracket on the opposite

side of the pump head.

4.Slide the platen back into the pump head frame assembly until it rests up
against the tubing. Press the cam lever in toward the pump head, lock­i

ng the platen up against the tubing and rollers. Note that the platen can

be inserted with the cam lever on the left or the right.

5.



Proper adjustment of platen pressure increases flow stability, minimizes
flow pulsation, and prolongs the life of the tubing.

6.Attach system tubing to the pump tubing using Bio-Rad's luer-type fit­tings or other suitable connectors. Lock-rings are not necessary for sys­tem tubing.

5.3 Installation of Uncut Tubing

Tubing length and the amount of tubing prestretch have a significant

effect on both flow rate calibration and reproducibility of flow. The PharMed
tubing supplied with the Model EP-1 Econo Pump has been cut to a prede
termined length to accommodate tubing prestretch. When using any tubing
that is not properly sized, the tubing must be cut to accommodate prestretch.
Tubing should be cut as follows:

Note: Overtightening the platen adjustment screw will reduce flow rate

and shorten tubing life. If the platen is too loose, flow rate will decrease
as backpressure increases.

Tubing



Length

Tygon, PharMed



1

79 mm +/- 1.5 (7.04" +/- 0.05)

Silicone



171 mm +/- 1.5 (6.75" +/- 0.05)

Install tubing onto the pump head as described in Section 5.2.

Warning: If using tubing other than the type supplied by Bio-Rad, make
sure the wall thickness is not greater than 1.0 mm. Using tubing with a
greater wall thickness can damage the pump and void your warranty.

a.Loosen the platen adjustment screw, located on the front of the plat-

en, counterclockwise until the stop is reached.

Section 6
Operation

b.

Cut tubing to proper length and install fittings and lock-rings as
described in Section 5.2. Install tubing in pump as described in Figure

5.l. Start the pump and set flow rate to the value you expect to use for
your application.

c.

Turn adjustment screw clockwise just until uniform flow is achieved.
This will be evident by observing the uniform motion of bubbles in the
line (if any) or a motion-free inlet line when operating with the expect­ed backpressure.

Typically, one piece of silicone tubing will perform well with two turns

clockwise from stop. One piece of Tygon or PharMed tubing will perform

well between three and four turns clockwise from stop. When using two
pieces of tubing, approximately 50% more turn clockwise from stop will
be required. A clockwise stop will occur at eight turns clockwise.

6.1 Start-Up Procedure

1.Plug the Model EP-1 Econo Pump into an appropriate
grounded power source. Turn on the power switch on
the bottom of the unit. The pump will power up in the
Stop mode, with the LED display showing 0% of maxi
mum speed.

2.



With tubing in place, set the desired pump speed by
pressing the Arrow keys.

3.

Press the Run/Stop key to initiate flow. Press once more

to stop the flow. Flow rate will be displayed in percent
of maximum speed. Change the flow to the desired
speed while the pump is running by pressing the Arrow

1

0

11

keys.

6.3 Flow Rate Calibration

4.To change the direction of flow, stop the pump by press­i

ng the Run/Stop key. Press the Direction key to choose

the new direction. Restart by pressing the Run/Stop key.

5.To purge the tubing, press the Purge key. The pump will

run at 100% maximum speed. When the tubing is
primed, press the Purge key again to stop the purge
function and return to the Run mode, or press the
Run/Stop key to turn the pump off.

An uncalibrated Model EP- I Econo Pump will display pump speed as per­cent of maximum rpm. The user may also choose to select one of three tub­ing sizes for which calibration has been programmed at the factory. The
Model EP-1 Econo Pump also features a user calibration mode, which is
used when improved flow rate accuracy is desired, or when using non-stan­dard size tubing. The pump must be calibrated using one of the methods

described above before a fraction collection program may be entered.

Flow Rate Calibration with Pre-Selected Tubing Sizes (0.8, 1.6,

and 3.2 mm ID)

6.2 Verification of Econo System Software Version

The Model ES-1 Econo System Controller and Model EP-1 Econo Pump

should already be set up.

The Model EP-1 Econo Pump

must

have software version 2.01 or

higher to control the Model EV-1 Econo Buffer Selector.
If you do not have a Model EV-1 Econo Buffer Selector, continue with

Section 6.3. Otherwise check the software version of your system:

Simultaneously press and hold the Direction Key and the "down"
Arrow key on the front panel of the Model EP-1 Econo Pump. The
four digit LED display on the front panel of the Pump should dis-

play: "v2.01" or higher.

If your pump displays a number lower than 2.01,and you will be
using the Model EV-1 Econo Buffer Selector, your pump software
should be upgraded. Contact your local Bio-Rad representative for

details on how to upgrade software.
If your pump currently features software version 2.01 or higher

,
insure that all connections between the Model EP-1 Econo Pump,
Model ES-1 Econo System Controller, (and Model EV-1 Econo
Buffer Selector, if used) have been completed and the system power
is on.

1

2

User Calibration Procedure

1.With the pump in Stop mode, install tubing as described
in Section 5.2.

2.Press the key in the lower left-front of the pump panel
corresponding to the pump calibration feature, and con­tinue pressing until the indicator light corresponding to

either 0.8, l.6, or 3.2 mm ID tubing is lit.

3.The LED display reads ml/min for any of these pre-pro-

grammed tubing settings. If improved flow rate accuracy
is desired, see User Calibration Procedure below.

4.The pump is now automatically calibrated for the specif­ic tubing size. Press the Run/Stop key to start flow.
Increase or decrease the pump speed with the Arrow
keys.

1.



Install tubing as described in Section 5.2. Connect pump
inlet to a container of water or buffer. Connect a length

of tubing to the pump outlet.

2.Run pump to purge air from tubing, then stop pump.

Place the end of the outlet tubing in an empty graduated
cylinder.

3.Press Calibration key until "CAL" is displayed. Press the

Run/Stop key. Pump will run for 5 minutes at 25% of
maximum speed, and the display will count down time.

13

4.



When pump stops, read the volume of fluid on the grad­uated cylinder. Using the Arrow keys, enter this amount
on the display. Alternatively, when a suitable volume of
liquid has been collected, pressing Run/Stop will turn
off the timer and stop the pump. Enter volume collected
as described.

5.



When the value on the display matches the volume col­lected in the graduated cylinder, press Calibration. The

"CAL" indicator light will glow steadily, indicating that
the pump is calibrated. DO NOT select a tubing size at
this point, even if you are using one of the standard
sizes. The pump will "remember" the calibration key
even if it is switched off or disconnected from the power
line.

6.



Set desired flow rate using arrow keys.
Note: Flow rate depends on condition of tubing, platen

adjustment, and back pressure. For maximum flow rate
accuracy it may be necessary to recalibrate as the tubing
ages, when the tubing is replaced, if the platen is read-

justed, or if back pressure changes significantly.

6.4 Simple Time-Based Collection with the Model
2110 Fraction Collector

In addition to its features as a stand-alone pump, the Model EP-1 Econo
Pump is capable of controlling the Model 2110 Fraction Collector as well as
other fraction collectors (for operation with collectors other than the Model
2110 Fraction Collector, see Appendix B). The pump must be calibrated in
order to run a fraction collection scheme. See Section 5.3 for calibration.
Use Econo System Cable #l to connect the I/O

socket on the rear panel of
the fraction collector to the 8-pin connector on the rear of the Model EP-1
Econo Pump (see Figure 4.2).

1.



Press the Fraction Collector key on the pump front panel
to enter the fraction collector Edit mode. Note that the
fraction size and ml indicator lights are flashing,
prompting entry of fraction size. Enter the fraction size
in 0.l ml increments using the Arrow keys. After frac­tion size is selected, press the Fraction Collector key
once more to confirm the fraction size.

14

2.

3.

*Note: The Econo Pump will automatically shut off upon

reaching the total run volume (Vt).

4.

5.

6.

Enter a void volume (V) in I ml increments using the
Arrow keys. This feature allows the user to begin col­lecting fractions only after a pre-set volume of liquid
(i.e., the void volume) has passed through the column.
Press the Fraction Collector key to confirm this value. If
a void volume is not desired, enter 0.

Note: The optional Model SV-3 Diverter Valve (catalog
number 731-8235) is required to use the void volume

feature of the Model EP-1 Econo Pump.

Select the total run volume (V) in 1 ml increments using
the Arrow keys.* If zero is entered and confirmed, the
fraction collection scheme is disabled. To resume data
entry, see Step 1 above. All values previously entered
remain in the memory until other values are entered to
replace them.

After selecting a V

value, press the flashing Fraction
Collection key to enter the value. The flashing LED dis­play shows the estimated number of fractions to be col­lected.

Press the flashing Fraction Collection key once more to
enable the fraction collector. To actuate the fraction col­lector, press the Program Run key after starting the
pump. (The fraction collector can be programmed with
the pump running or stopped.) After the fraction collec­tion program is initiated, the LED display shows the
progression of the fraction collection scheme in minutes,
to the nearest tenth of a minute.

While a program is running, the user can use the Arrow
keys to display the ml of output, the progression of the
collection in min, the flow rate in ml/min, or the percent
of total run-time. If the Econo Pump is connected to the
Econo System Controller and the Gradient Former is
enabled, % buffer B can also be viewed while a program
is running.

15

9.When the fraction collection program is complete, the
pump stops and the display reads "END." Press any key
to return to the ready mode.

Notes on Fraction Collection:

1.After a fraction collection program has begun, the pump cannot be turned
off without terminating the fraction collection program.

2.During the V, period of the fraction collection scheme (as indicated by
the V, indicator light), the fraction collector is not active. To collect frac­tions during this void volume period, cancel V, by pressing the Fraction
Collector key. The fraction collector will start, with the LED display
indicating the time-progression of the collection scheme.

3.When the fraction collector is disabled by pressing the Fraction Collector
key, the fraction collector carousel will advance one tube.

4.When the fraction collector indicator light on the pump is lit, the Model
2110 Fraction Collector will be put into remote mode. Only the manual
advance keys will be active, and the Model 2110 display reads ---.

5.The fraction collection control feature of the Model EP-1 Econo Pump

can be used to turn off the pump even if a fraction collector is not con­nected. Simply enter 0 for fraction size. After confirming the V value
entered (the V light will be lit solid), start the pump, if not already run­ning; then press the flashing Program Run key. The LED display will
show time elapsed. The pump will stop automatically when the total run
volume has been delivered.

1

6

7.



To abort the fraction collection scheme at any time dur­i

ng the program, press the Run/Stop key twice. The
pump will stop, and the LED display will read OFF,
i

ndicating abortion of the fraction collection program.

Note: When a program is aborted, it can only be restart­ed from the beginning.

8.To place a collection program that has begun on Hold,
press the lit Program Run key once. The pump will stop
and the program will be held. To resume the collection
program, press once again. Pressing the Run/Stop key
while the program is on hold will abort the fraction col­lection scheme.

Use of the Diverter Valve

The optional Model SV-3 diverter valve (catalog number 731-8235) pro­vides a means to divert the eluant stream from a fraction collector to a waste
receptacle or other collection device during a fraction collection program. It
fits conveniently to the Econo System Rack. If the void volume function
(V

o

) of the collection scheme is enabled with the diverter valve in place, the
eluant stream will be diverted away from the fraction collector during that peri­od.

When that period is over, the eluant stream will be diverted to the frac-

tion collector.

Whenever the diverter valve changes from waste to collect, or
vice versa, an event mark is generated. This will appear as a deflection on the
chart recorder. For connection of the diverter valve, see Figure 6.l.

Fig. 6.1. Connection of the Model SV-3 Diverter Valve.

17

Section 7

Cleaning and Maintenance

Section 8

Troubleshooting

18 19

The Model EP-1 Econo Pump requires very little maintenance to assure
reliable operation. The procedures outlined below will insure maximum
pump life:

•Check tubing regularly for signs of cracking and wear. If any exist,

replace the tubing.

•



For optimal tubing life, use a slow to medium pump speed. To increase

throughput, increase tubing size to obtain the desired output.

•When not in use, tubing should be removed from the pump to prevent

deformation of the tubing. Remove the platen and relax the tubing by
unhooking one end of the tubing from the tubing bracket.

•To prevent the formation of precipitates around the pump head and on the

membrane key panel, promptly remove any spills. Clean with deionized
water.

Problem

Possible Cause Solution

Pump displays

Power surge or

l.

Turn the power switch off.

"OUT"

brownout

2.

Press and hold down the
(up arrow) key and the (CAL)
key simultaneously while turning
the power switch on. This will
reset the pump. If the pump was
previously calibrated you will
have to recalibrate it.

Pump displays

Power outage

If you wish to continue a run in

"FAIL"

progress, press (program run)
key. Otherwise, press the
(run/stop) key.

Pump will not run

No power

Verify that powercord is plugged

(no LEDs lit)

in and the power switch is turned
on.

Fuses

Check that proper fuses are
installed and intact.

Problem

Possible Cause

Solution

Insure that the calibration indica­torlight is not flashing. For pump
to run, this light must either be
off or steadily lit.

Liquid remains

Platen not adjusted

Platen too loose. Turn platen

stationary in tubing

screw clockwise just until liquid
begins moving within tubing.

Clogged tubing, Check tubing, fittings, and valves
valves, or fittings

for obstructions.

Damaged tubing

Check pump tubing for damage.
Replace damaged tubing.

Flow rate not

Pump tubing

Replace tubing.

consistent

damaged or worn out

Platen adjustment

Adjust platen.

incorrect

Wrong tubing size Re-select tubing size on front
selected

panel of pump.

Large change in

Re-calibrate flow.

back pressure or
fluid viscosity

Restriction on inlet

Remedy

side

Excessive tubing

Platen too tight

Turn platen adjustment screw

wear

clockwise to stop flow, then
clockwise until flow starts.

Cannot calibrate

Pump must be Insure that pump is stopped.

pump

stopped in order to
calibrate pump

Error message

Contact Bio-Rad Instrument

displayed upon

Service Department

powering up (ER ##)

OIL

Appendix A

2

0

Appendix B

Operation of the Model EP-1 Econo Pump When
Connected to Other Instruments

The Model EP-1 Econo Pump provides a variety of output signals which
can be used to control the operation of non Bio-Rad fraction collectors, or to
communicate with non Bio-Rad UV monitors and recording devices.
Communication is accomplished through two standard 8-pin mini-DIN sock­ets on the rear panel of the pump. The third socket is for the operation of
the Model SV-3 Diverter Valve (see Figure 4.2). Bio-Rad offers an accesso­ry breakout cable (Econo System cable #7), which has an 8-pin mini-DIN con­nector at one end and loose wires on the other, for connection to non Econo
System instruments. Output signals are TTL compatible. To use these signals,
you must insure that the circuit external to the Model EP-1 Econo Pump
does not draw more than 15 milliamperes of current. The following table
describes the two rear panel mini-DIN connector pinouts.

Table B. Rear Panel Pinouts

21

Technical Specifications

Number of channels



2

Flow rate range (per channel)



0.1-20 ml/min (depending on tub­i

ng diameter)

Pump head speed



25 rpm maximum

Tubing diameter



0.4 mm ID to 3.2 mm ID

Speed adjustment



1

Speed stability



1

% of full scale

Maximum counterpressure



30 psi (2 kg/cm2 or bars)

Motor



DC speed controlled, 55 watts

Line voltage



90-132 VAC

180-265 VAC

47-63 Hz

Dimensions



143 x 202 x 222 mm

(

W x D x H) with pumphead

Weight



3.3 kg

Operating temperature



4 to 40 °C

Material of construction



polypropylene and other solvent
resistant plastics

Pinout

Signal Type

Pinout

Signal Type

Shield Shield

1

Fraction Advance

1

Start/Enable

(low-going output)

(output)

2

No Contact

2

No Contact

3

No Contact

3

Mark A (output)

4

No Contact 4 No Contact

5

Fraction Advance

5 No Contact

(high-going output)

6

No Contact 6

Pen (output)

7

Ground

7

Ground

8

Fraction Advanced Marks to

8

Mark B (output)

Recorder (low-going input)

Appendix C

Ordering Information

Catalog
Number



Product Description

Model EP-1
Econo Pump

731-8140Model EP-1 Econo Pump, 110 V (USA power cord), with

tubing set and starter fittings kit

731-8142



Model EP-1 Econo Pump, 220 V (no power cord), with

tubing set and starter fittings kit

731-8145Model EP-1 Pumphead Assembly

731-8235



Model SV-3 Diverter Valve
Tubing and Accessories
731-8210Silicone Tubing, 0.8 mm ID, 0.8 mm wall, 10 m
731-8211Silicone Tubing, 1.6 mm ID, 0.8 mm wall, 10 m
731-8212Silicone Tubing, 3.2 mm ID, 0.8 mm wall, 10 m

731-8214



Tygon®Tubing, 0.8 mm ID, 0.8 mm wall, 10 m

731-8215Tygon Tubing, 1.6 mm ID, 0.8 mm wall, 10 m

731-8207PharMed Tubing, 0.8 mm ID, 1.0 mm wall, 10 m
731-8208PharMed Tubing, 1.6 mm ID, 1.0 mm wall, 10 m
731-8219PharMed Tubing, 3.2 mm ID, 1.0 mm wall, 10 m

731-8240Silicone Tubing Kit, 0.8 mm ID, 20 precut lengths and 4

sets of fittings

731-8241Silicone Tubing Kit, 1.6 mm ID, 20 precut lengths and 4

sets of fittings

731-8242



Silicone Tubing Kit, 3.2 mm ID, 20 precut lengths and 4
sets of fittings

731-8247PharMed Tubing Kit, 0.8 mm ID, 20 precut lengths and 4

sets of fittings

731-8248PharMed Tubing Kit, 1.6 mm ID, 20 precut lengths and 4

sets of fittings

731-8249



PharMed Tubing Kit, 3.2 mm ID, 20 precut lengths and 4
sets of fittings

22

Catalog

Number



Product

Descri

ption

731-8228Low Pressure Fittings Kit, includes over 250 male and

female luer connectors, 2- and 3-way stopcocks, and tub­i

ng connectors

Cables
731-8261Econo System Cable 1, 8-pin mini-DIN to DB-9 connector.

For connection of Model 2110 Econo Fraction Collector to
the Econo Pump, Econo UV Monitor, or Econo System
Controller

731-8262Econo System Cable 2, 8-pin mini-DIN to 8-pin standard

DIN. For connection of Econo Recorders to the Econo UV
Monitor, Econo Pump, Econo Gradient Monitor, or Econo
System Controller

731-8263Econo System Cable 3, 8-pin mini-DIN to 8-pin mini DIN.

Connects the Econo Pump to the Econo UV Monitor in the
absence of the System Controller

731-8264Econo System Cable 4, 8-pin mini-DIN to banana cable.

To connect Econo UV Monitor to most non-Bio-Rad chart

recorders

731-8265Econo System Cable 5, DB-9 connector to bare wires. For

connection of Model 2110 Econo Fraction Collector to non­Econo System components

731-8266Econo System Cable 6, 8-pin standard DIN to bare wires.

To connect Econo Recorder to non-Econo System detec­tors

731-8267Econo System Cable 7, 8-pin mini-DIN to bare wires. To

connect Econo Pump, Econo UV Monitor, or Econo System
Controller to non-Econo System components

731-8268Econo System Cable 8, 8-pin standard DIN to DB-9 con-

nector. For connection of Econo Recorders to Econo
Fraction Collector

731-8269



Econo System Cable 9, 8-pin mini-DIN to Pharmacia Frac-

100. For connection of Pharmacia Frac-100 fraction collec-

tor to the Econo System

731-8281Econo System Cable 10, 8-pin mini-DIN to 11-pin connec-

tor . For connection of the Model EV-1 Econo Buffer
Selector to the Econo System Controller.

PharMed and Tygon are the registered trademarks of the Norton Company.

23