Bio-Rad EP-1 User Manual
Model EP-1
Econo Pump
Instruction
Manual
Catalog Numbers
731-8140
731-8142
For Technical Service
Call Your Local Bio-Rad Office or
in the U.S. Call 1-800-4BIORAD
(1-800-424-6723)
Warranty
Model _________________________________________
Serial Number ___________________________________
Date of Delivery _________________________________
Warranty Period _________________________________
The Model EP-1 Econo Pump is warranted for 1 year against defects in
materials and workmanship.
If any defects should occur during this warranty period, Bio-Rad will
replace the defective parts without charge. However, the following defects
are specifically excluded:
1. Defects caused by improper operation.
2. Repair or modification done by anyone other than Bio-Rad
Laboratories or their authorized agent.
3. Use with tubings or fittings not specified by Bio-Rad Laboratories for
use with this pump.
4. Deliberate or accidental misuse.
5. Damage caused by disaster.
6. Damage due to use of improper solvent or sample.
This warranty does not apply to tubing, fittings, and fuses.
For inquiry or request for repair service, contact Bio-Rad Laboratories
after confirming the model and serial number of your instrument.
For Technical Service Call Your Local Bio-Rad Office or in the U.S.
Call 1-800-4BIORAD (1-800-424-6723).
Table of Contents
Section 1 Safety ..............................................................................1
Section 2 Introduction ...................................................................2
Section 3 Unpacking and Setting Up............................................3
3.1 Unpacking Instructions.............................................................3
3.2 Voltage Conversion...................................................................4
Section 4 Physical Description and Control Features ................5
4.1 Front Panel Functions...............................................................5
4.2 Rear Panel Functions................................................................7
4.3 Pump Base Features .................................................................8
Section 5 Tubing Selection and Installation ................................8
5.1 Tubing Selection.......................................................................8
5.2 Installation of Precut Bio-Rad Tubing .....................................9
5.3 Installation of Uncut Tubing...................................................10
Section 6 Stand Alone Operation ................................................11
Section 7 Operation with other Instruments.............................12
7.1 Use of the Pump with the Model 2110
Fraction Collector ...................................................................13
7.2 Use of the Econo Pump with the Model 2128
Fraction Collector ...................................................................16
7.3 Use of the Econo Pump with the
BioLogic System ....................................................................17
Section 8 Cleaning and Maintenance.........................................17
Section 9 Troubleshooting ...........................................................18
Section 10 Embedded Functions...................................................19
Appendix A Technical Specifications ..............................................20
Appendix B Operation of the Econo Pump When
Connected to Other Instruments ...............................21
Appendix C Ordering Information .................................................22
Section 1
Safety
Disconnect supply before servicing. No user serviceable parts inside,
refer servicing to Bio-Rad service personnel.
This instrument is intended for laboratory use only.
This product conforms to the “Class A” standards for electromagnetic
emissions intended for laboratory equipment applications. It is possible
that emissions from this product may interfere with some sensitive
appliances when placed nearby or in the same circuit as those
appliances. The user should be aware of this potential and take
appropriate measures to avoid interference.
1
!
Section 2
Introduction
Fig. 2.1. Model EP-1 Econo Pump.
The Model EP-1 Econo Pump is a two-channel, bi-directional, variable
speed peristaltic pump for low-pressure chromatography and general
laboratory use. It offers a full range of features to facilitate ease of use as a
stand-alone pump or as a part of the BioLogic System. As a stand-alone
pump, the Model EP-1 Econo Pump delivers flow rates from 0.1 to 40
ml/min with the ability to self-calibrate the flow rate for 0.8, 1.6, and 3.2
mm ID tubing, displaying pump output in ml/min. The pump can be
programmed to control fraction collector parameters such as fraction size,
void volume, and total run volume. A membrane key panel with graphic
icon displays allows easy user interface.
2
Section 3
Unpacking and Setting Up
3.1 Unpacking Instructions
Carefully remove the unit from the shipping box, lifting from the
handle on the back of the pump or from the bottom of the instrument.
Remove the plastic bag and inspect the instrument for any external damage.
Parts included with the Model EP-1 Econo Pump are illustrated in Figure
3.1. Check off all parts against the supplied packing list.
Your Model EP-1 Econo Pump was carefully tested at the factory and
was shipped in good working order. If any part is missing or damaged,
contact Bio-Rad Laboratories immediately. Refer to Figure 3.1 for the
proper identification designation of any missing or damaged part(s).
Also includes: Instruction Manual
Fig. 3.1. Parts supplied with the Model EP-1 Econo Pump.
3
Tubing and Fitting
Starter Kit
Power Cord
Model EP-1 Econo Pump
3.2 V oltage Conversion
Warning: The Model EP-1 Econo Pump is shipped in its 120 V or
220 V version. To operate at other voltages, refer to the procedure
below. Failure to follow this procedure may result in damage to the unit
and invalidation of the warranty.
Prior to connecting the power cord to the power entry module and wall
outlet, verify that the voltage indicated on the power entry module matches
your line voltage. If it does not, use the following procedure to make the
conversion. Refer to Figure 3.2.
1. Disconnect the power cord from the unit.
2. Remove the fuse drawer with a small-blade screwdriver or similar tool.
3. Pull the fuse holder out of the fuse drawer and, if necessary, replace the
fuses with ones having the correct current rating. Use 0.50 A fuses for
100 V and 120 V operation; 0.25 A fuses for 220 V and 240 V operation.
Rotate the voltage selector through the window in the fuse drawer until
the proper voltage shows.
4. Reinsert the fuse drawer into the power entry module, with the locking
tab to the left. The voltage indicator will read right-side-up if the drawer
is oriented correctly. Press gently until it snaps into place.
5. Insert the power cord plug into the power entry module. Plug the power
cord into a properly grounded outlet.
Fig. 3.2.Voltag e conversion.
4
Fuse Drawer
Voltage Selector
Power
Entry Module
Window
Section 4
Physical Description and Control Features
4.1 Front Panel Functions
Fig. 4.1. Front panel controls.
Display Function
LED Display The four-character display will show
various parameters during programming
and operation of the Econo Pump. The
indicator lights located immediately to
the right of the display indicate the units
displayed: ml, minutes, ml/minute, or
percent.
Arrow Keys These keys are used for setting system
parameters when programming the
system, and for selecting which
parameter to display when running the
pump.
ml
min
ml/min
%
0.8
1.6
3.2
CAL
V
V
t
o
ECONO PUMP
5
Indicator Lights These lights indicate the units for the
value displayed on the LED display
located to the left. A flashing light
indicates that a value can be set using
the Arrow keys.
Run/Stop Key This key is used to start or stop the
pump. It also has secondary functions
such as holding or aborting a method.
Purge Key This key is used to prime and purge
tubing lines without disturbing the
primary speed setting of the pump.
When Purge is pressed, the pump will
run at maximum speed (25 rpm). The
purge key will not operate during a
fraction collection method.
Direction Key This key changes the direction of
pump head rotation. The Econo Pump
must be stopped before the direction
can be changed.
Tubing Calibration Key This key is used to select pre-pro-
grammed calibration settings for three
tubing sizes, or allows user calibration
for increased flow rate accuracy or
non-standard tubing sizes. The pump
must be stopped for this key to operate.
Fraction Collector Key This key is used to set fraction size,
void volume (to move a diverter valve
to "collect" or "waste" positions), and
total run volume. The total run volume (Vt) may be used to program the
pump to automatically shut off after a
given volume has been delivered.
ml
6
min
ml/min
%
0.8
1.6
3.2
CAL
V
V
o
t
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