Bio-Rad ENrich High-Resolution Size Exclusion Columns User Manual

ENrich™ SEC 70
ENrich™ SEC 650
High-Resolution
Size Exclusion Columns

Instruction Manual

Catalog numbers

780-1070
780-1650

Please read these instructions before you use ENrich SEC
high-resolution size exclusion media. If you have any questions or
comments regarding these instructions, please contact your
Bio-Rad Laboratories representative.

Table of Contents

Section 1: Characteristics of the ENrich

Size Exclusion Columns .......................................... 1

1.1 Introduction .............................................................1

1.2 The ENrich SEC Separation Media ........................... 1

1.3 Connection to the NGC™ and Other

Chromatography Systems ....................................... 2

Section 2 : Use of the ENrich SEC Columns ............................ 3

2.1 Preparation for Initial Use ......................................... 3

2.2 Equilibrating and Running the Column ..................... 3

2.3 Sample Considerations ............................................ 4

2.4 Sample Elution ......................................................... 4

2.5 Column Calibration .................................................. 4

Section 3 : Care of the ENrich SEC Columns ........................... 6

3.1 Regular Maintenance ............................................... 6

3.2 Bed Height Adjustment ............................................ 6

3.3 Column Cleaning ..................................................... 7

3.4 Frit Replacement ..................................................... 7

3.5 Storage Conditions .................................................. 9

Section 4 : Ordering Information ..............................................10

Section 1: Characteristics of the
ENrich™ Size Exclusion Columns

1.1 Introduction

ENrich prepacked columns for size exclusion chromatography are
designed for rapid and reproducible high-resolution separation of
biomolecules. Each column contains a unique, high-performance
size exclusion media based on spherical polymer beads modified
to provide quick, differential size–based diffusion.

1.2 The ENrich SEC Separation Media

ENrich SEC media is available with different exclusion limits
and separation ranges. The prepacked columns contain 10 μm
beads, which produce excellent resolution of biomolecules at low
backpressures. SEC 70 is ideal for separating biomolecules up
to 70,000 Da and SEC 650 is used for larger biomolecules up to
650,000 Da.

The 10 μm particle size and narrow particle size distribution
provide excellent resolution of biomolecules at high flow rates and
with relatively low backpressures. The hydrophilic ENrich media
demonstrates extremely low nonspecific binding of biomolecules
accompanied by high recovery of biological activity.

Stability

The columns are stable over the pH range 2–12, allowing easy
cleaning and regeneration.

ENrich SEC Instruction Manual 1