Bio-Rad ENrich High-Resolution Ion Exchange Columns User Manual
ENrich™ Q
ENrich
™
S
High-Resolution Ion
Exchange Columns
Instruction Manual
Catalog numbers
780-0001
780-0003
780-0021
780-0023
Please read these instructions before you use ENrich Q and S
high-resolution ion exchange media. If you have any questions or
comments regarding these instructions, please contact your
Bio-Rad Laboratories representative.
Table of Contents
Section 1: Characteristics of the ENrich Ion
Exchange (IEX) Columns ................................... 1
1.1 Introduction .......................................................1
1.2 The ENrich Separation Media ............................. 1
1.3 Connection to the NGC™ and Other
Chromatography Systems ................................. 2
Section 2 : Use of the ENrich IEX Columns ........................ 3
2.1 Preparation for Initial Use ................................... 3
2.2 Buffer Selection ................................................. 3
2.3 Sample Preparation ........................................... 5
2.4 Elution Conditions .............................................. 5
Section 3 : Care of the ENrich IEX Columns ....................... 7
3.1 Column Cleaning ............................................... 7
3.2 Bed Height Adjustment ...................................... 8
3.3 Frit Replacement ............................................... 9
3.4 Storage Conditions ...........................................12
Section 4 : Product Information .........................................13
Section 1: Characteristics of the
ENrich Ion Exchange Columns
1.1 Introduction
ENrich prepacked ion exchange columns meet the needs of the
biochromatographer for rapid and reproducible high-resolution
separations of proteins and other biomolecules, including
peptides and polynucleotides. Two column sizes (bed volumes
of 1 and 8 ml) provide predictable scale-up of high-resolution
separations without sacrificing capacity.
1.2 The ENrich Separation Media
Each column contains a spherical, rigid, and highly porous
polymeric support derivatized with the strongly basic –N+(CH3)3
quaternary ammonium group or the strongly acidic –SO
The 10 μm particle-size and narrow particle size distribution
provide excellent resolution of biomolecules at high flow rates
and with low backpressures. The hydrophilic ENrich media
demonstrate extremely low non-specific binding of biomolecules
accompanied by high recovery of biological activity.
Stability
The columns are stable over a 2–12 pH range, allowing easy
cleaning and regeneration.
The ENrich support is compatible with aqueous solutions of 6 M
guanidine-HCl and 8 M urea. Nonionic detergents and organic
solvents such as methanol, ethanol, and isopropanol may also
be used. Ionic detergents can be used only if the detergents
have the same charge as the support (that is, sodium dodecyl
sulfate (SDS), a negatively charged detergent, should not be
used with a positively charged anionic exchanger like ENrich Q).
–
group.
3
ENrich Instruction Manual 1
Table 1. Column characteristics.
Q S
Column dimensions
(diameter x height, mm)
Column volume, ml 1 8 1 8
Maximum protein capacity, mg
Bovine serum albumin 130 1000 – –
Human IgG – – 120 940
Average particle size, μm 10 10 10 10
Maximum operating pressure,
psi
Recommended flow
rate, ml/min*
* At room temperature. Viscosity may increase at lower temperatures, which will
reduce the recommended flow rates.
5 x 50 10 x 100 5 x 50 10 x 100
500 500 500 500
1 4 1 4
1.3 Connection to the NGC and Other
Chromatography Systems
The ENrich columns are fitted with 10-32 type female fittings on either
end. Standard 10-32 fittings can be used to plumb the column to
Bio-Rad’s NGC chromatography system or other vendors’ systems.
Adaptors are available for connection to systems that use 1/4-28
fittings (catalog # 750-0564).
2 ENrich Instruction Manual
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