Xylem E-Line 80 User Manual

E-Line 80 Refractometer

User Guide

E-Line 80 Refractometer User Guide (Eng)

B+S Code : 44-893

Issue 1C

January 2010

Table 1
Order Code Range Scale Division Scale No. Scale Ranges

© Copyright Bellingham + Stanley Ltd 2010

Every effort has been made to ensure the accuracy of the contents of this manual. However, Bellingham +
Stanley Ltd can assume no responsibility for errors contained in the manual or their consequences.

Bellingham + Stanley pursues a policy of continuous product development and improvement and as such,
information given in this manual may be updated or withdrawn without notice. Some publicity materials prior
to January 2010 will not reflect such product improvements, noticeably the change in colour of the instrument
barrel from black to bright silver. This change does not affect the performance of the instrument under normal
operating conditions.

Printed in United Kingdom.

Bellingham+Stanley Ltd. Bellingham+Stanley Inc.
Longfield Road, Tunbridge Wells, 1000 Hurricane Shoals Road, Building D,
Kent, TN2 3EY, United Kingdom Suite 300, Lawrenceville, GA 30043, USA
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]
[e-mail] [e-mail]

1 0-50 °Brix 44-807 E-Line 80 0.5 °Brix minimum
2 50-80 °Brix

Product Description

The E-Line 80 is a dual scale Brix refractometer ideal for use in the food industry and other
applications requiring concentration measurements over a wide measurement range.

Applying the sample to the refractometer:
Lift the illuminating prism
view the borderline (light/dark demarcation line) through the eyepiece

Eyepiece

Optical glass is relatively soft and care should be taken not to scratch the prism surface.
Do not use metal spatulas or glass rods to apply samples but instead use softer materials
such as plastic.

Always check sample Health & Safety Data before applying to the refractometer.

When applying samples to the prism which are likely to cause harm to skin or eyes, wear
appropriate protective clothing and glasses.

Taking a reading:
Depending on the concentration of the sample, select the range of measurement
the scale selection control
ranges until a clear borderline is found.

The concentration of the sample can be measured at the point the borderline crosses the scale.
Take care to ensure that the reading is taken from the correct scale range.

Refractive Index values can be obtained by using a conversion table available from Bellingham +
Stanley Ltd, see Table 3.

1

and apply the sample to the measurement prism2. Close the prism and

3

Scale Selection Cotrol

Zero Calibration 5Measurement

4

Prism

4

. If the concentration is unknown, apply the sample and step through the

3

.

2

Illuminating

1

Prism

(Table 1)

by adjusting

Page 1

Cleaning the prism:
Thoroughly clean the prism after use with water or other suitable solvent and dry with clean tissue.
The prism surface could be damaged by strong alkalis or acids if left in contact for long periods of
time. Clean samples from the prism as soon as practicable. Wiping the prism surface occasionally
with alcohol will remove any build-up of oils left from the samples.

Correcting the reading for temperature:
The refractometer is calibrated to read correctly at 20˚C. The refractive index of the measured
sample will vary with temperature and the reading must be corrected to take account of this.
Please use the Table 2 below to adjust your reading accordingly.

Temperature Correction Table (Table 2)
This table gives mass fraction corrections to refractometric tables for sucrose solutions at 589nm for
temperature different from 20˚C. Taken from ICUMSA, Appendix 2, SPS-3 (1998) page 8.

Temp.

(°C)

Measured Sucrose ˚Brix (Mass Fraction)

0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85

15 -0.29 -0.30 -0.32 -0.33 -0.34 -0.35 -0.36 -0.37 -0.37 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.37 -0.37

16 -0.24 -0.25 -0.26 -0.27 -0.28 -0.28 -0.29 -0.30 -0.30 -0.30 -0.31 -0.31 -0.31 -0.31 -0.31 -0.30 -0.30 -0.30

17 -0.18 -0.19 -0.20 -0.20 -0.21 -0.21 -0.22 -0.22 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.22

18 -0.12 -0.13 -0.13 -0.14 -0.14 -0.14 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15

19 -0.06 -0.06 -0.07 -0.07 -0.07 -0.07 -0.07 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.07

20 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

21 0.06 0.07 0.07 0.07 0.07 0.07 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.07

22 0.13 0.14 0.14 0.14 0.15 0.15 0.15 0.15 0.16 0.16 0.16 0.16 0.16 0.16 0.15 0.15 0.15 0.15

23 0.20 0.21 0.21 0.22 0.22 0.23 0.23 0.23 0.23 0.24 0.24 0.24 0.24 0.23 0.23 0.23 0.23 0.22

24 0.27 0.28 0.29 0.29 0.30 0.30 0.31 0.31 0.31 0.32 0.32 0.32 0.32 0.31 0.31 0.31 0.30 0.30

25 0.34 0.35 0.36 0.37 0.38 0.38 0.39 0.39 0.40 0.40 0.40 0.40 0.40 0.39 0.39 0.38 0.38 0.37

26 0.42 0.43 0.44 0.45 0.46 0.46 0.47 0.47 0.48 0.48 0.48 0.48 0.48 0.47 0.47 0.46 0.46 0.45

27 0.50 0.51 0.52 0.53 0.54 0.55 0.55 0.56 0.56 0.56 0.56 0.56 0.56 0.55 0.55 0.54 0.53 0.52

28 0.58 0.59 0.60 0.61 0.62 0.63 0.64 0.64 0.64 0.65 0.65 0.64 0.64 0.63 0.63 0.62 0.61 0.60

29 0.66 0.67 0.68 0.70 0.71 0.71 0.72 0.73 0.73 0.73 0.73 0.73 0.72 0.72 0.71 0.70 0.69 0.67

30 0.74 0.76 0.77 0.78 0.79 0.80 0.81 0.81 0.82 0.82 0.81 0.81 0.80 0.80 0.79 0.78 0.76 0.75

31 0.83 0.84 0.85 0.87 0.88 0.89 0.89 0.90 0.90 0.90 0.90 0.89 0.89 0.88 0.87 0.86 0.84 0.82

32 0.92 0.93 0.94 0.96 0.97 0.98 0.98 0.99 0.99 0.99 0.99 0.98 0.97 0.96 0.95 0.93 0.92 0.90

33 1.01 1.02 1.03 1.05 1.06 1.07 1.07 1.08 1.08 1.08 1.07 1.07 1.06 1.04 1.03 1.01 1.00 0.98

34 1.10 1.11 1.13 1.14 1.15 1.16 1.16 1.17 1.17 1.16 1.16 1.15 1.14 1.13 1.11 1.09 1.07 1.05

35 1.19 1.21 1.22 1.23 1.24 1.25 1.25 1.26 1.26 1.25 1.25 1.24 1.23 1.21 1.19 1.17 1.15 1.13

36 1.29 1.30 1.31 1.33 1.34 1.34 1.35 1.35 1.35 1.34 1.34 1.33 1.31 1.29 1.28 1.25 1.23 1.20

37 1.39 1.40 1.41 1.42 1.43 1.44 1.44 1.44 1.44 1.43 1.43 1.41 1.40 1.38 1.36 1.33 1.31 1.28

38 1.49 1.50 1.51 1.52 1.53 1.53 1.54 1.54 1.53 1.53 1.52 1.50 1.48 1.46 1.44 1.42 1.39 1.36

39 1.59 1.60 1.61 1.62 1.63 1.63 1.63 1.63 1.63 1.62 1.61 1.59 1.57 1.55 1.52 1.50 1.47 1.43

40 1.69 1.70 1.71 1.72 1.73 1.73 1.73 1.73 1.72 1.71 1.70 1.68 1.66 1.63 1.61 1.58 1.54 1.51

Page 2

Calibration:
The E-line 80 is calibrated to read accurately at 20˚C. Readings may be taken at any other
temperature by applying a correction factor (Table 2).

Alternatively the E-line 80 has the facility to correct for temperature by manual offset. Prior to
taking a reading, ensure the temperature of the instrument is stable. Apply distilled water and check
that the reading is zero (Scale 1). If the reading is not zero, use the screwdriver supplied to adjust
the zero calibration screw

5

so that the borderline crosses the zero mark on the scale. No
temperature correction is required for this method but as the relationship between refractive index
and temperature is not linear, small errors may occur at the top end of the scale compared to
readings near water.

Care Warning:

This refractometer is a precision optical instrument and should be handled with care. Do
not drop or subject it to sharp knocks.

Page 3

°Brix to Refractive Index Conversion Table (Table 3)
The table below can be used to convert °Brix readings taken from the instrument scale into
refractive index.

°Brix

Refractive Index at

589.3nm and 20.0°C

0 1.33299 30 1.38115 60 1.44193

1 1.33442 31 1.38296 61 1.44420

2 1.33586 32 1.38478 62 1.44650

3 1.33732 33 1.38661 63 1.44881

4 1.33879 34 1.38846 64 1.45113

5 1.34026 35 1.39032 65 1.45348

6 1.34175 36 1.39220 66 1.45584

7 1.34325 37 1.39409 67 1.45822

8 1.34477 38 1.39600 68 1.46061

9 1.34629 39 1.39792 69 1.46303

10 1.34782 40 1.39986 70 1.46546

11 1.34937 41 1.40181 71 1.46790

12 1.35093 42 1.40378 72 1.47037

13 1.35250 43 1.40576 73 1.47285

14 1.35408 44 1.40776 74 1.47535

15 1.35568 45 1.40978 75 1.47787

16 1.35729 46 1.41181 76 1.48040

17 1.35891 47 1.41385 77 1.48295

18 1.36054 48 1.41592 78 1.48552

19 1.36218 49 1.41799 79 1.48811

20 1.36384 50 1.42009 80 1.49071

21 1.36551 51 1.42220

22 1.36720 52 1.42432

23 1.36889 53 1.42647

24 1.37060 54 1.42862

25 1.37233 55 1.43080

26 1.37406 56 1.43299

27 1.37582 57 1.43520

28 1.37758 58 1.43743

29 1.37936 59 1.43967

°Brix

Refractive Index at

589.3nm and 20.0°C

°Brix

Refractive Index at

589.3nm and 20.0°C

Data Source: ICUMSA Methods Book, Specification and Standard SPS-3 (2000), Refractometry

and Tables - (Official)

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